13,493 results match your criteria recorded vivo

In Vivo Comparison of Radiation Exposure in Third Generation versus Second Generation Dual-Source Dual-Energy CT for Imaging Urinary Calculi.

J Endourol 2021 Apr 15. Epub 2021 Apr 15.

Mayo Clinic Florida, 23389, Radiology, Jacksonville, Florida, United States;

Purpose: To investigate the potential for decreasing radiation dose when utilizing a third generation versus second generation dual-source dual-energy CT scanner, while maintaining diagnostic image quality and acceptable image noise.

Materials And Methods: Retrospective analysis of patients who underwent dual-source dual-energy CT (dsDECT) for clinical suspicion of urolithiasis from 10/2/2017 - 9/5/2018. Patient demographics, body mass index, abdominal diameter, scanning parameters, and CT dose index volume (CTDIvol) were recorded. Read More

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Shengma Biejia Decoction Inhibits Cell Growth in Multiple Myeloma by Inducing Autophagy-Mediated Apoptosis Through the ERK/mTOR Pathway.

Front Pharmacol 2021 29;12:585286. Epub 2021 Mar 29.

Department of Hematology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Shengma Biejia decoction (SMBJD), a traditional Chinese formula recorded in the , has been widely used for the treatment of malignant tumors. However, its underlying molecular targets and mechanisms are still unclear. This study showed that SMBJD inhibited tumor growth and stimulated hemogram recovery significantly in a multiple myeloma xenograft model. Read More

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Attenuation of Hippocampal Evoked Potentials by Activation of GtACR2, an Optogenetic Chloride Channel.

Front Neurosci 2021 29;15:653844. Epub 2021 Mar 29.

4BRAIN Team, Department of Head and Skin, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Aim: GtACR2, a light-activated chloride channel, is an attractive tool for neural inhibition as it can shunt membrane depolarizations. In this study, we assessed the effect of activating GtACR2 on hippocampal CA1 activity evoked by Schaffer collateral (SC) stimulation.

Methods: Adult male Wistar rats were unilaterally injected with 0. Read More

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Heliconia rostrata rhizomes mitigate chemical-induced liver injury by debilitating oxidative stress in HepG2 cells and rats.

J Ethnopharmacol 2021 Apr 11:114113. Epub 2021 Apr 11.

Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Siksha O Anusnadhan Deemed to Be University, Kalinga Nagar, Bhubaneswar, Odisha, 751003, India. Electronic address:

Ethnopharmacological Relevance: Heliconia rostrata Ruiz and Pav. belongs to the family Heliconiaceae. Plant was traditionally used to cure jaundice, intestinal pain, diabetes and hypertension. Read More

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Comparative study on the diagnostic value of intravenous/peritumoral injection of indocyanine green for metastatic lymph node location in patients with head and neck squamous cell carcinoma (HNSCC).

Ann Transl Med 2021 Mar;9(6):507

Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.

Background: Accurate assessment of regional lymph node (LN) status is essential for the treatment of head and neck squamous cell carcinoma (HNSCC) patients. In this study, we aimed to compare the difference between intravenous injection of indocyanine green (ICG) and peritumoral injection of ICG in the location of metastatic LNs.

Methods: Twenty-nine patients were enrolled in this study with 13 patients receiving intravenous injection of ICG and 16 patients receiving peritumoral injection of ICG. Read More

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Increased beat-to-beat variability of cerebral microcirculatory perfusion during atrial fibrillation: a near-infrared spectroscopy study.

Europace 2021 Apr 13. Epub 2021 Apr 13.

Division of Cardiology, Department of Medical Sciences, 'Città della Salute e della Scienza di Torino' Hospital, University of Turin, Turin, Italy.

Aims: Atrial fibrillation (AFib) is associated with cognitive decline/dementia, independently from clinical strokes or transient ischaemic attacks (TIA). Recent in silico data suggested that AFib may induce transient critical haemodynamic events in the cerebral microcirculation. The aim of this study is to use non-invasive spatially resolved cerebral near-infrared spectroscopy (SRS-NIRS) to investigate in vivo beat-to-beat microcirculatory perfusion during AFib and after sinus rhythm (SR) restoration. Read More

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Interferon-γ Response of subsp. Infected Goats to Recombinant and Synthetic Mycobacterial Antigens.

Front Vet Sci 2021 26;8:645251. Epub 2021 Mar 26.

Animal and Plant Health Agency, Addlestone, United Kingdom.

Despite its potential for early diagnosis of subsp. (MAP) infection, the IFN-γ release assay is not used routinely, because of low specificity of the established crude antigen preparation Johnin (PPDj). Limited data are available assessing the potential of MAP-derived protein and lipopeptide antigens to replace PPDj in assays for goats, while cattle and sheep have been studied more extensively. Read More

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An integrative review on the toxicity of Bisphenol A (BPA) released from resin composites used in dentistry.

J Biomed Mater Res B Appl Biomater 2021 Apr 8. Epub 2021 Apr 8.

Department of Dental Sciences, University Institute of Health Sciences (IUCS), CESPU, Gandra, PRD, Portugal.

The main aim of this study was to perform an integrative review on the release of bisphenol A (BPA) from resin-matrix composites and potential toxic effects. A bibliographic search was performed on the PubMed platform using the following keywords: "Bisphenol A" OR "BPA" AND "resin composite" OR "composite resin" AND "toxicity" OR "cytotoxicity" OR "release". Inclusion criteria involved in vitro and in vivo studies on the release and toxicity of BPA. Read More

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Pterocephin A, a novel Triterpenoid Saponin from Pterocephalus hookeri induced liver injury by activation of necroptosis.

Phytomedicine 2021 Mar 19;85:153548. Epub 2021 Mar 19.

College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China. Electronic address:

Background: Pterocephalus hookeri (C. B. Clarke) Höeck, a Tibetan medicine widely used for treatment of rheumatoid arthritis, was recorded in Chinese Pharmacopoeia (2020 version) with slight toxicity. Read More

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In vitro and in vivo relaxation and anti-inflammation of Natural Flavonoids from Elaeagnus pungens leaf via L-type Calcium channel and Targeting MAPK signal pathway.

J Ethnopharmacol 2021 Apr 5:114071. Epub 2021 Apr 5.

School of Pharmaceutical Science, South-Central University for Nationalities, Wuhan, 430074, P.R. China. Electronic address:

Ethnopharmacological Relevance: In traditional Chinese medicine (TCM), the leaf of Elaeagnus pungens Thunb. (Family Elaeagnaceae) is an herb documented as an antiasthmatic remedy to treat the severe asthma, bronchitis and other respiratory diseases in the early material medica "Bencao Gangmu" (Ming dynasty, about 442 years ago).

Aim Of The Study: This work is purposed to investigate the pharmacological effects and mechanism of total flavonoids from Elaeagnus pungens leaves (FLA) on asthma in vivo and vitro. Read More

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Pendimethalin-based herbicide impairs cellular immune response and haemocyte morphology in a beneficial ground beetle.

J Insect Physiol 2021 Apr 6;131:104236. Epub 2021 Apr 6.

Department of Biology, Ecology and Earth Science, University of Calabria, Italy. Electronic address:

Herbicides have become the most commonly applied agrochemicals in agroecosystems. Thus, basic knowledge of their physiological effects on insects is needed, especially for understanding their impact on beneficial insect species. In this study, we evaluated the effect of a pendimethalin-based herbicide (PND) on the cellular immune response of the carabid beetle Harpalus (Pseudoophonus) rufipes (De Geer 1774) (Coleoptera, Carabidae), acting as biocontrol agent in agroecosystems. Read More

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Clinical symptoms of limited exercise capacity linked to aai-ddd functionality: an in silico and in vivo approach.

Pacing Clin Electrophysiol 2021 Apr 8. Epub 2021 Apr 8.

Cardiovascular Research Center, Onze-Lieve-Vrouw Hospital Aalst, Moorselbaan 164, Aalst, B-9300, Belgium.

Background: Exercise capacity is an important aspect of quality of life in patients undergoing pacemaker implantation. Device algorithms for ventricular pacing avoidance have been developed to avoid unnecessary and potentially harmful effects of right ventricular pacing. However, little data exists on the immediate response of these algorithms to sudden AV block during exercise. Read More

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Ex Vivo Brain Preparation to Analyze Vocal Pathways of Frogs.

Ayako Yamaguchi

Cold Spring Harb Protoc 2021 Apr 7. Epub 2021 Apr 7.

School of Biological Sciences, University of Utah, Salt Lake City, Utah 84112-0840, USA

Understanding the neural basis of behavior is a challenging task for technical reasons. Most methods of recording neural activity require animals to be immobilized, but neural activity associated with most behavior cannot be recorded from an anesthetized, immobilized animal. Using amphibians, however, there has been some success in developing in vitro brain preparations that can be used for electrophysiological and anatomical studies. Read More

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J Zoo Wildl Med 2021 Apr;52(1):300-305

Center For Wildlife Health, Joe Johnson Boulevard, University of Tennessee, Knoxville, TN 37996, USA.

() is an important fungal pathogen present in wild hellbender () populations that appears to cause disease during novel exposure and acute stress. Hellbender repatriation efforts are ongoing to combat declining populations, but mortality by chytridiomycosis (disease from ) after release has been reported. The goal was to determine whether a safe antifungal agent could be administered and provide prolonged plasma concentrations without repeated handling. Read More

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Subconjunctival administration of low-dose murine allogeneic mesenchymal stromal cells promotes corneal allograft survival in mice.

Stem Cell Res Ther 2021 Apr 6;12(1):227. Epub 2021 Apr 6.

College of Medicine, Nursing and Health Sciences, Biomedical Sciences, Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland.

Background: Systemic administration of mesenchymal stromal cells (MSCs) has been efficacious in many inflammatory disease settings; however, little data are available on the potential immunomodulatory effects following local MSC administration in the context of corneal transplantation. The purpose of this study was to assess the potential of subconjunctival injection of MSCs to promote corneal allograft survival.

Methods: MSCs were isolated from female C57BL/6 (H-2) or Balb/c (H-2) mice and extensively characterized. Read More

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Conformal Radiotherapy in Patients with Cardiovascular Implantable Electronic Devices: Proposed Practical Implementation of the TG-203 Risk-Stratified Interrogation Schedule.

Pract Radiat Oncol 2021 Apr 3. Epub 2021 Apr 3.

Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD. Electronic address:

Introduction: Radiotherapy (RT) in patients with cardiac implantable electronic devices (CIED) carries a risk of device malfunction from radiation exposure. We sought to evaluate the incidence of CIED malfunction in a cohort of patients treated with modern RT techniques.

Material And Methods: A retrospective analysis of 193 CIED patients treated with RT between 2000 and 2018 was conducted. Read More

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Development and validation of a novel microfluidic device for the manipulation of skeletal muscle microvascular blood flow in vivo.

Microcirculation 2021 Apr 4:e12698. Epub 2021 Apr 4.

Division of BioMedical Sciences, Memorial University of Newfoundland, 300 Prince Philip Drive HSC 4311, St. John's, NL, A1B 3V6, Canada.

Objective: To develop and validate a novel liquid microfluidic approach to deliver drugs to microscale regions of tissue while simultaneously allowing for visualization and quantification of microvascular blood flow.

Methods: Microfluidic devices were fabricated using soft lithographic techniques, molded in polydimethylsiloxane, and bound to a coverslip with a 600x300μm micro-outlet. Sprague-Dawley rats, anaesthetized with pentobarbital, were instrumented to monitor systemic parameters. Read More

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Value of endoscopy with narrow-band imaging and probe-based confocal laser endomicroscopy in the diagnosis of preneoplastic lesions of gastrointestinal tract.

Rom J Morphol Embryol 2020 Jul-Sep;61(3):759-767

Department of Scientific Research Methodology and Department of Pulmonology, University of Medicine and Pharmacy of Craiova, Romania;

Introduction: Amongst all malignant tumors, cancers of the digestive tract rank first in terms of yearly deaths. Patients above 60 years of age are the most affected, as the diagnosis is frequently made in advanced stages of the disease when therapy is less effective. Our study aimed to evaluate the efficiency of narrow-band imaging (NBI) endoscopy and probe-based confocal laser endomicroscopy (pCLE) in the correct diagnosis of preneoplastic lesions in the upper and lower digestive tract. Read More

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Mucoadhesive In Situ Rectal Gel Loaded with Rifampicin: Strategy to Improve Bioavailability and Alleviate Liver Toxicity.

Pharmaceutics 2021 Mar 5;13(3). Epub 2021 Mar 5.

Department of Chemistry, College of Science, Jouf University, Sakaka P.O. Box 2014, Saudi Arabia.

Although it is a front-line in tuberculosis treatment, rifampicin (RF) exhibits poor oral bioavailability and hepatotoxicity. Rectal mucoadhesive and in situ rectal gels were developed to overcome drug drawbacks. A RF/polyethylene glycol 6000 co-precipitate was first prepared in different ratios. Read More

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Hypoxic Conditions Promote Rhythmic Contractile Oscillations Mediated by Voltage-Gated Sodium Channels Activation in Human Arteries.

Int J Mol Sci 2021 Mar 4;22(5). Epub 2021 Mar 4.

PhyMedExp, Université de Montpellier, INSERM, CNRS, 34495 Montpellier, France.

Arterial smooth muscle exhibits rhythmic oscillatory contractions called vasomotion and believed to be a protective mechanism against tissue hypoperfusion or hypoxia. Oscillations of vascular tone depend on voltage and follow oscillations of the membrane potential. Voltage-gated sodium channels (Na), responsible for the initiation and propagation of action potentials in excitable cells, have also been evidenced both in animal and human vascular smooth muscle cells (SMCs). Read More

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Subnormothermic Ex Vivo Lung Perfusion Temperature Improves Graft Preservation in Lung Transplantation.

Cells 2021 Mar 29;10(4). Epub 2021 Mar 29.

Department of Thoracic Surgery, University Hospital Zurich, Rämistrasse 100, 8091 Zurich, Switzerland.

Normothermic machine perfusion is clinically used to assess the quality of marginal donor lungs. Although subnormothermic temperatures have proven beneficial for other solid organ transplants, subnormothermia-related benefits of ex vivo lung perfusion (EVLP) still need to be investigated. In a rat model, we evaluated the effects of 28 °C temperature on 4-h EVLPs with subsequent left lung transplantation. Read More

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In vivo measurement of distance between scapular neck and polyethylene insert during active external rotation in shoulders with Grammont type reverse prosthesis.

Clin Biomech (Bristol, Avon) 2021 Mar 26;84:105341. Epub 2021 Mar 26.

Department of Mechanical & Aerospace Engineering, University of Florida, 330 MAE-A P.O. Box 116250, Gainesville, FL 32611-6250, USA.

Background: Scapular notching is a frequently observed complication after reverse shoulder arthroplasty. Impingement of the humeral plastic insert against the scapular neck is believed to be the cause of notching. There have been no in vivo studies that analyzed the positional relationship between the scapular neck and humeral insert. Read More

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A novel investigation method for axonal damage in neuromyelitis optica spectrum disorder: In vivo corneal confocal microscopy.

Mult Scler J Exp Transl Clin 2021 Jan-Mar;7(1):2055217321998060. Epub 2021 Mar 19.

Koc University Research Center for Translational Medicine (KUTTAM), Istanbul, Turkey.

Background: Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory autoimmune disorder that damages optic nerves, brainstem, and spinal cord. In vivo corneal confocal microscopy (IVCM) is a noninvasive technique that provides corneal images with dendritic cells (DCs) and corneal subbasal nerve plexus (SBP), which arises from the trigeminal nerve.

Objective: We investigated corneal SBP changes in NMOSD and proposed IVCM as a potential new disease severity biomarker for NMOSD. Read More

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Chloroplast-derived photo-oxidative stress causes changes in H2O2 and EGSH in other subcellular compartments.

Plant Physiol 2021 Jan 6. Epub 2021 Jan 6.

Institute of Crop Science and Resource Conservation (INRES), University of Bonn, D-53113 Bonn, Germany.

Metabolic fluctuations in chloroplasts and mitochondria can trigger retrograde signals to modify nuclear gene expression. Mobile signals likely to be involved are reactive oxygen species (ROS), which can operate protein redox switches by oxidation of specific cysteine residues. Redox buffers, such as the highly reduced glutathione pool, serve as reservoirs of reducing power for several ROS-scavenging and ROS-induced damage repair pathways. Read More

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January 2021

High data rate communications in vivo using ultrasound.

IEEE Trans Biomed Eng 2021 Apr 1;PP. Epub 2021 Apr 1.

The emergence of in-body medical devices has provided a means of capturing physiological or diagnostic information and streaming this information outside of the body. Currently, electromagnetic-based communications make up the bulk of in-body medical device communication protocols. Traditional electromagnetic-based solutions are limited in their data rates and available power. Read More

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Investigations on the effects of anti-Leishmania major serum on the progression of Leishmania infantum infection in vivo and in vitro - implications of heterologous exposure to Leishmania spp.

Parasitol Res 2021 Apr 1. Epub 2021 Apr 1.

Laboratory of Cellular Immunology & Parasitology, Department of Biological Sciences, Central Washington University, 400E, University Way, Ellensburg, WA, 98926, USA.

Leishmaniasis is a vector-borne parasitic disease caused by protozoa of the genus Leishmania. Twenty different species are known to cause disease in humans with varying degrees of pathology. These diseases are transmitted throughout the geographic range of phlebotomine sandflies, found between the latitudes 50°N and 40°S. Read More

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Transcriptomic and metabonomic profiling reveal the anti-obesity effects of Chikusetsusaponin V, a compound extracted from Panax japonicus.

J Pharm Pharmacol 2021 Mar;73(1):60-69

TCM and Ethnomedicine Innovation & Development International Laboratory, Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, PR China.

Objectives: To explore the in vivo anti-obesity effect of chikusetsusaponin V and explore the underlying mechanism by transcriptomic and metabonomic methods.

Methods: The physiological parameters of high-fat-diet induced obese mice administered with or without 25 mg/kg and 100 mg/kg of chikusetsusaponin V by gavage for 16 weeks were recorded. In addition, the RNA-sequencing and UHPLC-Q-TOF techniques were applied to obtain the transcriptomic and metabolomic profiling, respectively. Read More

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Patterns of Limb and Epaxial Muscle Activity During Walking in the Fire Salamander, .

Integr Org Biol 2020 27;2(1):obaa015. Epub 2020 May 27.

Structure and Motion Laboratory, Department of Comparative Biomedical Sciences, The Royal Veterinary College, Hawkshead Lane, Hatfield, AL9 7TA, UK.

Salamanders and newts (urodeles) are often used as a model system to elucidate the evolution of tetrapod locomotion. Studies range from detailed descriptions of musculoskeletal anatomy and segment kinematics, to bone loading mechanics and inferring central pattern generators. A further area of interest has been muscle activity patterns, measured through electromyography (EMG). Read More

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The feasibility of multimodal fiber optic spectroscopy analysis in bladder cancer detection, grading, and staging.

Urologia 2021 Mar 31:3915603211007018. Epub 2021 Mar 31.

Unit of Minimally Invasive and Robotic Urologic Surgery and Kidney Transplantation, Careggi University Hospital, Florence, Italy.

Objective: To prove the feasibility of Multimodal Fiber Optic Spectroscopy (MFOS) analysis in bladder cancer (BCa) detection, grading, and staging.

Materials And Methods: Bladder specimens from patients underwent TURBT or TURP were recorded and analyzed with MFOS within 30 min from excision. In detail, our MFOS combined fluorescence, Raman spectroscopy, and diffuse reflectance. Read More

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[Guiding mechanism of platycodin D in treatment of mouse lung cancer with doxorubicin].

Zhongguo Zhong Yao Za Zhi 2021 Mar;46(6):1480-1489

Zhengzhou University of Industrial Technology Xinzheng 451150,China Pharmaceutic College of Henan University Kaifeng 475000,China.

This study is to observe whether platycodin D has the guiding role in treatment of mouse lung cancer with doxorubicin and explore its guiding mechanism. In vitro, platycodin D and doxorubicin(alone or in combination) were added into Lewis lung cancer(LLC) cells to detect the cell proliferation and doxorubicin uptake. Cell morphological changes were analyzed by cell holographic analysis system; cell gap junctional intercellular communication(GJIC) was tested by fluorescent yellow tracer; lyso-tracker red was used to examine lysosomal function; LC-3 B(Light chain 3 beta)and P62(heat shock 90-like protein)staining were used to test auto-phagy and autophagic degradation respectively; and P-glycoprotein(P-gp) expression was examined by Western blot. Read More

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