155 results match your criteria recognized lactococcus


Rapid Isolation and Purification of Secreted Bacteriocins from and Other Lactic Acid Bacteria.

Bio Protoc 2020 Nov 20;10(22):e3824. Epub 2020 Nov 20.

Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.

Bacteriocins are small ribosomally synthesized antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for low pH. Bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. Bacteriocins produced by LAB are primarily active against closely related bacterial species. Read More

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November 2020

Plasmid Replicons for the Production of Pharmaceutical-Grade pDNA, Proteins and Antigens by Cell Factories.

Int J Mol Sci 2021 Jan 30;22(3). Epub 2021 Jan 30.

iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.

The bacterium found in different natural environments is traditionally associated with the fermented food industry. But recently, its applications have been spreading to the pharmaceutical industry, which has exploited its probiotic characteristics and is moving towards its use as cell factories for the production of added-value recombinant proteins and plasmid DNA (pDNA) for DNA vaccination, as a safer and industrially profitable alternative to the traditional host. Additionally, due to its food-grade and generally recognized safe status, there have been an increasing number of studies about its use in live mucosal vaccination. Read More

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January 2021

TLR-Mediated Cytokine Gene Expression in Chicken Peripheral Blood Mononuclear Cells as a Measure to Characterize Immunobiotics.

Genes (Basel) 2021 Jan 29;12(2). Epub 2021 Jan 29.

Department of Animal Biotechnology and Genetics, UTP University of Science and Technology, 85-084 Bydgoszcz, Poland.

Immunobiotics are probiotics that promote intestinal health by modulating immune responses. Immunobiotics are recognized by Toll-like receptors (TLRs) and activate cytokine gene expression. This study aimed to characterize cytokine gene expression in the chicken peripheral blood mononuclear cells (PBMC) stimulated with purified TLR ligands and live probiotics. Read More

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January 2021

Global transcriptomic analysis of functional oligosaccharide metabolism in Pediococcus pentosaceus.

Appl Microbiol Biotechnol 2021 Feb 29;105(4):1601-1614. Epub 2021 Jan 29.

Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

Lactic acid bacteria (LAB) are important in food fermentation and may enhance overall host health. Previous studies to explore LAB metabolism mainly focused on the genera Lacticaseibacillus and Lactococcus. Pediococcus pentosaceus, historically recognized as an important food fermentation bacterial strain, can produce bacteriocins and occasionally demonstrated probiotic functionalities. Read More

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February 2021

Lectin-Mediated Binding of Engineered to Cancer Cells.

Microorganisms 2021 Jan 22;9(2). Epub 2021 Jan 22.

Department of Biotechnology, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.

Lectins have been increasingly utilized as carriers for targeted drug delivery based on their specific binding to glycans located on mammalian cells. This study employed two lectins, B subunit of bacterial Shiga holotoxin (Stx1B) and fungal lectin (CNL), for surface display on the lactic acid bacterium . The specific adhesion of these engineered, lectin-displaying to cancer cells was evaluated. Read More

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January 2021

Novel Pathway for Vanadium(V) Bio-Detoxification by Gram-Positive .

Environ Sci Technol 2021 Feb 25;55(3):2121-2131. Epub 2021 Jan 25.

School of Water Resources and Environment, MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, China University of Geosciences (Beijing), Beijing 100083, P. R. China.

Whereas prospects of bioremediation for a vanadium(V) [V(V)]-contaminated environment are widely recognized, reported functional species are extremely limited, with the vast majority of Gram-negative bacteria in Proteobacteria. Herein, the effectiveness of V(V) reduction is proved for the first time by , a Gram-positive bacterium in Firmicutes. The V(V) removal efficiency was 86. Read More

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February 2021

Novel Strategies for Efficient Production and Delivery of Live Biotherapeutics and Biotechnological Uses of : The Lactic Acid Bacterium Model.

Front Bioeng Biotechnol 2020 4;8:517166. Epub 2020 Nov 4.

Laboratory of Cellular and Molecular Genetics, Federal University of Minas Gerais, Belo Horizonte, Brazil.

Lactic acid bacteria (LAB) are traditionally used in fermentation and food preservation processes and are recognized as safe for consumption. Recently, they have attracted attention due to their health-promoting properties; many species are already widely used as probiotics for treatment or prevention of various medical conditions, including inflammatory bowel diseases, infections, and autoimmune disorders. Some LAB, especially , have been engineered as live vehicles for delivery of DNA vaccines and for production of therapeutic biomolecules. Read More

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November 2020

Probiotics-Derived Peptides and Their Immunomodulatory Molecules Can Play a Preventive Role Against Viral Diseases Including COVID-19.

Probiotics Antimicrob Proteins 2020 Nov 23. Epub 2020 Nov 23.

Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.

As of recent, the pandemic episode of COVID-19, a severe acute respiratory syndrome brought about by a novel coronavirus (SARS-CoV-2) expanding the pace of mortality, has affected the disease rate profoundly. Invulnerability is the fundamental choice to prevent the ruining event of COVID-19, as the drugs and antibodies are in the phase of preliminary clinical trials. Within this brief period, a few strains of SARS-CoV-2 have been recognized by the vaccine manufacturers, which could be an incorrect guess about the strain that will end up spreading. Read More

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November 2020

Complete Genome Sequence of Lactococcus lactis subsp. G121, an Isolate with Allergy-Protective Features Derived from a Farming Environment.

Microbiol Resour Announc 2020 Oct 8;9(41). Epub 2020 Oct 8.

Research Unit for Comparative Microbiome Analysis, Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany

Early childhood exposure to a farming environment has been found to be protective against asthma and other atopic disorders. Here, we report the complete genome sequence of subsp. G121, which was isolated from the kitchen of a farm in Bavaria (Germany) and is recognized for its allergy-protective properties. Read More

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October 2020

Participation of the intestinal microbiota in the mechanism of beneficial effect of treatment with synbiotic Syngut on experimental colitis under stress conditions.

J Physiol Pharmacol 2020 Jun 26;71(3). Epub 2020 Sep 26.

Department of Medicine 1, Hector-Center for Nutrition, Exercise and Sports, Erlangen-Nurnberg (FAU), Germany.

Gut-brain axis plays a central role in the regulation of stress related diseases such as irritable bowel syndrome (IBS) or inflammatory bowel disease (IBD). It is increasingly recognized that stress modulates gut microbiota community structure and activity and represents an important causal factor in dysbiosis. This study was designed to determine the effect of daily treatment with synbiotic (Syngut) containing inulin, Lactobacillus acidophilus, Bifidobacterium lactis W51, Lactobacillus plantarum W21 and Lactococcus lactis applied i. Read More

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Longitudinal Metatranscriptomic Analysis of a Meat Spoilage Microbiome Detects Abundant Continued Fermentation and Environmental Stress Responses during Shelf Life and Beyond.

Appl Environ Microbiol 2020 11 24;86(24). Epub 2020 Nov 24.

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland

Microbial food spoilage is a complex phenomenon associated with the succession of the specific spoilage organisms (SSO) over the course of time. We performed a longitudinal metatranscriptomic study on one modified-atmosphere-packaged (MAP) beef product to increase understanding of the longitudinal behavior of a spoilage microbiome during shelf life and beyond. Based on the annotation of the mRNA reads, we recognized three stages related to the active microbiome that were descriptive of the sensory quality of the beef: acceptable product (AP), early spoilage (ES), and late spoilage (LS). Read More

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November 2020

Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources.

Methods Mol Biol 2021 ;2220:243-257

School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia.

This chapter describes methods used to isolate, identify, and partially characterize lactic acid bacteria (LAB) which exhibit inhibitory activity against Listeria monocytogenes from foods. Vegetal (plant based) sources are rich in naturally occurring LAB and therefore provide an easily accessible source of strains with potential antimicrobial activity for use in food-processing applications. From our previous work, the majority of LAB with inhibitory activity against L. Read More

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Overview of exopolysaccharides produced by Weissella genus - A review.

Int J Biol Macromol 2020 Dec 25;164:2964-2973. Epub 2020 Aug 25.

Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India. Electronic address:

Exopolysaccharides (EPS) from lactic acid bacteria (LAB) are much diversed in structure, composition and applications which also adding a great commercial potential due to its generally recognized as safe (GRAS) status. LAB genus such as Lactobacillus, Leuconostoc, Streptococcus, Weissella, Lactococcus are known to produce EPS. Among this genus, Weissella is enormously reported for diversity and high production of EPS with wide range of industrial applications and bio-functional properties. Read More

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December 2020

Angiotensin-I-converting enzyme inhibitory peptides in milk fermented by indigenous lactic acid bacteria.

Vet World 2020 Feb 21;13(2):345-353. Epub 2020 Feb 21.

Department of Food Science and Technology, Food Science Study Program, IPB University (Bogor Agricultural University), Bogor, Indonesia.

Background And Aim: Fermented milk can be used to produce antihypertensive peptides. Lactic acid bacteria (LAB) with its proteolytic system hydrolyze milk protein during fermentation to produce several peptides, which include antihypertensive bioactive peptides. This study aimed to investigate the ability of indigenous LAB for the production of angiotensin-I-converting enzyme inhibitory (ACE-I) peptides in fermented milk and to characterize the ACEI peptides. Read More

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February 2020

Targeting Melanoma Hypoxia with the Food-Grade Lactic Acid Bacterium .

Cancers (Basel) 2020 Feb 13;12(2). Epub 2020 Feb 13.

Department of Surgery, School of Medicine, University of Louisville, Louisville, KY 40202, USA.

Melanoma is the most aggressive form of skin cancer. Hypoxia is a feature of the tumor microenvironment that reduces efficacy of immuno- and chemotherapies, resulting in poor clinical outcomes. is a facultative anaerobic gram-positive lactic acid bacterium (LAB) that is Generally Recognized as Safe (GRAS). Read More

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February 2020

Aggregation-prone peptides modulate activity of bovine interferon gamma released from naturally occurring protein nanoparticles.

N Biotechnol 2020 Jul 3;57:11-19. Epub 2020 Feb 3.

Institute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain; Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain; Bioengineering, Biomaterials and Nanomedicine Networking Biomedical Research Centre (CIBER-BBN), Bellaterra, Barcelona, Spain. Electronic address:

Efficient protocols for the production of recombinant proteins are indispensable for the development of the biopharmaceutical sector. Accumulation of recombinant proteins in naturally-occurring protein aggregates is detrimental to biopharmaceutical development. In recent years, the view of protein aggregates has changed with the recognition that they are a valuable source of functional recombinant proteins. Read More

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Surface display of uropathogenic Escherichia coli FimH in Lactococcus lactis: In vitro characterization of recombinant bacteria and its protectivity in animal model.

Microb Pathog 2020 Apr 8;141:103974. Epub 2020 Jan 8.

Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave, Tehran, 13164, Iran. Electronic address:

Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are very common, leading to high patient morbidity and substantial medical costs. The development of non-antibiotic strategies such as food-grade lactic acid bacterium can be recognized as an attractive and safe alternative way against UTI. Here, we report the construction of Lactococcus lactis (L. Read More

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A food-grade engineered Lactococcus lactis strain delivering Helicobacter pylori Lpp20 alleviates bacterial infection in H. pylori-challenged mice.

Biotechnol Lett 2019 Dec 16;41(12):1415-1421. Epub 2019 Oct 16.

Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, China.

Objective: To construct a food-grade bacterium producing and delivering H. pylori Lpp20 antigen and evaluate its immune efficacy against H. pylori challenges with aim to develop anti-H. Read More

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December 2019

Taking insight into the gut microbiota of three spider species: No characteristic symbiont was found corresponding to the special feeding style of spiders.

Ecol Evol 2019 Jul 23;9(14):8146-8156. Epub 2019 Jun 23.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, School of Life Sciences Hubei University Wuhan China.

Microorganisms in insect guts have been recognized as having a great impact on their hosts' nutrition, health, and behavior. Spiders are important natural enemies of pests, and the composition of the gut microbiota of spiders remains unclear. Will the bacterial taxa in spiders be same as the bacterial taxa in insects, and what are the potential functions of the gut bacteria in spiders? To gain insight into the composition of the gut bacteria in spiders and their potential function, we collected three spider species, , and , in the field, and high-throughput sequencing of the 16S rRNA V3 and V4 regions was used to investigate the diversity of gut microbiota across the three spider species. Read More

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Secretion and functional expression of Mycobacterium bovis antigens MPB70 and MPB83 in lactic acid bacteria.

Tuberculosis (Edinb) 2019 07 29;117:24-30. Epub 2019 May 29.

School of Biosciences and Medicine, University of Surrey, Guildford, GU2 7XH, UK. Electronic address:

The aim of this study was to determine the reliability of lactic acid bacteria (LAB) as heterologous hosts for the expression of MPB70 and MPB83, two Mycobacterium bovis antigens that possess diagnostics and immunogenic properties, respectively. We therefore generated recombinant cells of Lactococcus lactis and Lactobacillus plantarum that carried hybrid genes encoding MPB70 and MPB83 fused to signal peptides that are specifically recognized by LAB. Only L. Read More

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A Specific Sugar Moiety in the Lactococcus lactis Cell Wall Pellicle Is Required for Infection by CHPC971, a Member of the Rare 1706 Phage Species.

Appl Environ Microbiol 2019 10 17;85(19). Epub 2019 Sep 17.

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands

is a Gram-positive bacterium widely used as a starter culture for the production of different dairy products, especially a large variety of cheeses. Infection of lactococcal starter cultures by bacteriophages is one of the major causes of fermentation failure and often leads to production halt. Lactococcal bacteriophages belonging to the c2, 936, and P335 species are the most commonly isolated in dairy plants and have been extensively investigated in the past three decades. Read More

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October 2019

Enhanced gastrointestinal survivability of recombinant Lactococcus lactis using a double coated mucoadhesive film approach.

PLoS One 2019 23;14(7):e0219912. Epub 2019 Jul 23.

Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia.

Vaccine administration via the oral route is preferable to parenteral routes due to ease of administration. To date, most available oral vaccines comprises of live attenuated pathogens as oppose to peptide-based vaccines due to its low bioavailability within the gastrointestinal (GI) tract. Over the years, probiotic-based peptide delivery vehicles comprising of lactic acid bacteria such as Lactococcus lactis has emerged as an interesting alternative due to its generally recognized as safe (GRAS) status, a fully sequenced genome, transient gut colonization time, and is an efficient cellular factory for heterologous protein production. Read More

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February 2020

Combining Modules for Versatile and Optimal Labeling of Lactic Acid Bacteria: Two pMV158-Family Promiscuous Replicons, a Pneumococcal System for Constitutive or Inducible Gene Expression, and Two Fluorescent Proteins.

Front Microbiol 2019 26;10:1431. Epub 2019 Jun 26.

Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Labeling of bacterial cells with fluorescent proteins allows tracking the bacteria in competition and interactomic and studies. During the last years, a few plasmid vectors have been developed aimed at the fluorescent labeling of specific members of the lactic acid bacteria (LAB), a heterogeneous group that includes microorganisms used in the food industry, as probiotics, or as live vectors for mucosal vaccines. Successful and versatile labeling of a broad range of LAB not only requires a vector containing a promiscuous replicon and a widely recognized expression system for the constitutive or regulated expression of the fluorescence determinant, but also the knowledge of the main features of the entire plasmid/host/fluorescent protein ensemble. Read More

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Bypassing lantibiotic resistance by an effective nisin derivative.

Bioorg Med Chem 2019 08 20;27(15):3454-3462. Epub 2019 Jun 20.

Institute of Biochemistry, Heinrich-Heine-University Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany; Center for Structural Studies, Heinrich-Heine-University Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany. Electronic address:

The need for new antibiotic compounds is rising and antimicrobial peptides are excellent candidates to fulfill this object. The bacteriocin subgroup lantibiotics, for example, are active in the nanomolar range and target the membranes of mainly Gram-positive bacteria. They bind to lipid II, inhibit cell growth and in some cases form pores within the bacterial membrane, inducing rapid cell death. Read More

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Production of clovamide and its analogues in Saccharomyces cerevisiae and Lactococcus lactis.

Lett Appl Microbiol 2019 Sep 16;69(3):181-189. Epub 2019 Jul 16.

Joint BioEnergy Institute, Emeryville, CA, USA.

Clovamide and its analogues are N-hydroxycinnamoyl-L-amino acids (HAA) that exhibit antioxidant activities. For environmental and economic reasons, biological synthesis of these plant-derived metabolites has garnered interest. In this study, we exploited HDT1, a BAHD acyltransferase recently isolated from red clover, for the production of clovamide and derivatives in S. Read More

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September 2019

Advanced Strategies for Food-Grade Protein Production: A New /Lactic Acid Bacteria Shuttle Vector for Improved Cloning and Food-Grade Expression.

Microorganisms 2019 Apr 27;7(5). Epub 2019 Apr 27.

National Research Council-Institute for the Study of Anthropic Impacts and Sustainability in the Marine Environment (IAS-CNR), Capo Granitola, Via del mare, Campobello di Mazara (TP), 91021 Sicily, Italy.

Food-grade production of recombinant proteins in Gram-positive bacteria, especially in LAB (i.e., , and ), is of great interest in the areas of recombinant enzyme production, industrial food fermentation, gene and metabolic engineering, as well as antigen delivery for oral vaccination. Read More

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Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site.

Biotechnol J 2019 Aug 20;14(8):e1800587. Epub 2019 May 20.

iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001, Lisboa, Portugal.

Plasmids for DNA vaccination are exclusively produced in the Gram-negative Escherichia coli. One important drawback of this system is the presence of lipopolysaccharides. The generally recognized as safe Lactococcus lactis (L. Read More

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GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier .

Front Microbiol 2019 18;10:527. Epub 2019 Mar 18.

Laboratory for Molecular Microbiology, The Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.

Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial health-promoting effects of GABA, the presence of the gene, encoding glutamate decarboxylase that converts L-glutamate to GABA, was analyzed in Lactic Acid Bacteria (LAB) natural isolates from Zlatar cheese. The results revealed that 52% of tested spp. Read More

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A New Bifidobacteria Expression SysTem (BEST) to Produce and Deliver Interleukin-10 in .

Front Microbiol 2018 21;9:3075. Epub 2018 Dec 21.

EA 4065, Ecosystème Intestinal, Probiotiques, Antibiotiques, Faculté de Pharmacie de Paris, DHU Risques et Grossesse, Université Paris Descartes, Paris, France.

In the last years there has been a growing interest in the use of genetically modified bacteria to deliver molecules of therapeutic interest at mucosal surfaces. Due to the well-recognized probiotic properties of some strains, bifidobacteria represent excellent candidates for the development of live vehicles to produce and deliver heterologous proteins at mucosal surfaces. However, very few studies have considered this genus because of its complexity to be genetically manipulated. Read More

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December 2018

Influence of the early bacterial biofilms developed on vats made with seven wood types on PDO Vastedda della valle del Belìce cheese characteristics.

Int J Food Microbiol 2019 Feb 19;291:91-103. Epub 2018 Nov 19.

Dipartimento Scienze Agrarie, Alimentari e Forestali, Università di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy. Electronic address:

Early vat bacterial biofilms developed spontaneously through contact with whey have been characterized on seven wood types (Castanea sativa Miller, Cedrus libani, A. Rich., Prunus avium L. Read More

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February 2019