3,472 results match your criteria recipient strain

Indole Inhibits IncP-1 Conjugation System Mainly Through Promoting and Expression.

Front Microbiol 2021 19;12:628133. Epub 2021 Mar 19.

Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, China.

Indole works as an interspecies signal molecule to regulate multiple physiological activities, like antibiotic resistance, acid resistance, and virulence. However, the effect of indole on conjugation is unknown. Here, with λπ as a donor strain that carries a chromosomally integrated conjugative RP4 plasmid, we explored the effect of indole on conjugation of a mobilizable pUCP24T plasmid imparting gentamycin resistance. Read More

View Article and Full-Text PDF

Genetic Characterization of AmpC and Extended-Spectrum Beta-Lactamase Phenotypes in and From Alberta Broiler Chickens.

Front Cell Infect Microbiol 2021 12;11:622195. Epub 2021 Mar 12.

Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.

Horizontal gene transfer is an important mechanism which facilitates bacterial populations in overcoming antimicrobial treatment. In this study, a total of 120 and 62 subsp. isolates were isolated from broiler chicken farms in Alberta. Read More

View Article and Full-Text PDF

Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi.

Sci Rep 2021 Mar 9;11(1):5462. Epub 2021 Mar 9.

Department of Bacteriology-I, National Institute of Infectious Disease, Toyama 1-23-1, Shinjuku, Tokyo, 162-8640, Japan.

Borrelia miyamotoi, a member of the tick-borne relapsing fever spirochetes, shows a serum-resistant phenotype in vitro. This ability of B. miyamotoi may contribute to bacterial evasion of the host innate immune system. Read More

View Article and Full-Text PDF

Extent of Cytomegalovirus Replication in the Human Host Depends on Variations of the HLA-E/UL40 Axis.

mBio 2021 03 16;12(2). Epub 2021 Mar 16.

Center for Virology, Medical University of Vienna, Vienna, Austria

Human cytomegalovirus (HCMV) may cause severe infections in lung transplant recipients (LTRs). In response to HCMV infections, a subset of NKG2C NK cells expands, which limits HCMV replication and is characterized by high expression of the activating NKG2C/CD94 and absence of the inhibitory NKG2A/CD94 receptor. Both receptors bind to HLA-E, which is stabilized by HCMV-encoded UL40 peptides. Read More

View Article and Full-Text PDF

Modifications of the mechanical properties of in vivo tissue-engineered vascular grafts by chemical treatments for a short duration.

PLoS One 2021 12;16(3):e0248346. Epub 2021 Mar 12.

Department of Cardiovascular Surgery, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.

In vivo tissue-engineered vascular grafts constructed in the subcutaneous spaces of graft recipients have functioned well clinically. Because the formation of vascular graft tissues depends on several recipient conditions, chemical pretreatments, such as dehydration by ethanol (ET) or crosslinking by glutaraldehyde (GA), have been attempted to improve the initial mechanical durability of the tissues. Here, we compared the effects of short-duration (10 min) chemical treatments on the mechanical properties of tissues. Read More

View Article and Full-Text PDF

Mobile Colistin Resistance Gene - Detected on an IncI2 Plasmid in Typhimurium Sequence Type 19 from a Healthy Pig in South Korea.

Microorganisms 2021 Feb 15;9(2). Epub 2021 Feb 15.

Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Korea.

Colistin is considered the last resort for the treatment of multi-drug resistant Gram-negative bacterial infections. We studied colistin resistance and the gene carriage in isolates recovered from food animals in South Korea between 2010 and 2018. Colistin resistance was found in 277 isolates, predominantly in Enteritidis (57. Read More

View Article and Full-Text PDF
February 2021

Conjugation Protocol Optimised for and .

Bio Protoc 2020 Apr 5;10(7):e3575. Epub 2020 Apr 5.

Rowett Institute of Nutrition and Health, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.

and species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of genetic manipulation techniques. Conjugative transposons previously introduced into species could not be easily modified, greatly limiting their applicability as genetic modification platforms. Read More

View Article and Full-Text PDF

Conjugation dynamics depend on both the plasmid acquisition cost and the fitness cost.

Mol Syst Biol 2021 03;17(3):e9913

Department of Biology, Barnard College, New York, NY, USA.

Plasmid conjugation is a major mechanism responsible for the spread of antibiotic resistance. Plasmid fitness costs are known to impact long-term growth dynamics of microbial populations by providing plasmid-carrying cells a relative (dis)advantage compared to plasmid-free counterparts. Separately, plasmid acquisition introduces an immediate, but transient, metabolic perturbation. Read More

View Article and Full-Text PDF

Conjugative DNA Transfer From to Transformation-Resistant Lactobacilli.

Front Microbiol 2021 11;12:606629. Epub 2021 Feb 11.

Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Universidad de Cantabria-CSIC-SODERCAN, Santander, Spain.

Lactic acid bacteria (LAB) belonging to the genus classically known as , recently split into 25 different genera, include many relevant species for the food industry. The well-known properties of lactobacilli as probiotics make them an attractive model also for vaccines and therapeutic proteins delivery in humans. However, scarce tools are available to accomplish genetic modification of these organisms, and most are only suitable for laboratory strains. Read More

View Article and Full-Text PDF
February 2021

Nonconservative integration and diversity of a new family of integrative and conjugative elements associated with antibiotic resistance in zoonotic pathogen Streptococcus suis.

Vet Microbiol 2021 Mar 19;254:109009. Epub 2021 Feb 19.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Macrolide and tetracycline resistance in streptococci is mainly caused by acquisition of integrative and conjugative elements (ICEs) of the ICESa2603 family carrying erm(B) and tet(O). But the characteristics about the transferability and physiological consequences of ICEs with triplet serine integrases are still rare. This study tested the transferability of ICESsuYZDH1_SSU0877, a novel erm(B)- and tet(O)-carrying ICESa2603 family-like ICE with triplet serine integrases, and evaluated the physiological consequences after ICE transferred and integrated into recipient. Read More

View Article and Full-Text PDF

Investigating the effects of municipal and hospital wastewaters on horizontal gene transfer.

Environ Pollut 2021 May 13;276:116733. Epub 2021 Feb 13.

Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden; Department of Infectious Diseases, Institute of Biomedicine, the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Electronic address:

Horizontal gene transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes. In sewer systems, human-associated and environmental bacteria are mixed together and exposed to many substances known to increase HGT, including various antibacterial compounds. In wastewaters, those substances are most often detected below concentrations known to induce HGT individually. Read More

View Article and Full-Text PDF

Carbapenem-resistant Enterobacteriaceae among kidney transplant recipients - insights on the risk of acquisition and CRE infection.

Infect Dis (Lond) 2021 Feb 20:1-10. Epub 2021 Feb 20.

Department of Infectious Diseases, University of São Paulo School of Medicine Hospital das Clínicas, São Paulo, Brazil.

Background: Kidney transplant recipients are a risk group for carbapenem-resistant Enterobacteriaceae infection.

Objectives: This study aimed to identify risk factors for CRE acquisition and infection among kidney transplant recipients.

Methods: We conducted a case-control study; we defined the case as kidney transplant recipient with positive culture for carbapenem-resistant Enterobacteriaceae identified between January 2010 and February 2019. Read More

View Article and Full-Text PDF
February 2021

Knowledge, Responsibilities, and Peer Advice From Care Partners of Patients With Parkinson Disease Psychosis.

Front Neurol 2021 1;12:633645. Epub 2021 Feb 1.

Department of Neurology, University of Pittsburgh, Pittsburgh, PA, United States.

Care partners (CPs) of individuals with Parkinson disease psychosis (PDP) experience increased strain and rely on informal support networks. The objective of this study was to characterize CP responsibilities, sources of support, and peer advice. This was a mixed-methods cross-sectional study. Read More

View Article and Full-Text PDF
February 2021

Cytomegalovirus antiviral stewardship in the COVID-19 Era: Increasing complexity of prophylaxis and treatment and potential mitigation strategies.

Transpl Infect Dis 2021 Feb 17:e13586. Epub 2021 Feb 17.

Department of Medicine, University of Wisconsin-Madison School of Medicine and Public Health, University of Wisconsin Hospital and Clinics, Madison, WI, USA.

Cytomegalovirus (CMV) infection is one of the most common and significant complications after solid organ transplant (SOT). Severe acute respiratory coronavirus 2 (SARS-CoV-2), which causes the novel betacoronavirus 2019 disease (COVID-19), has become the first global pandemic in 100 years. The world's attention has turned to address this unanticipated development; however, the viral infection that has long plagued outcomes after solid organ transplantation still requires vigilance. Read More

View Article and Full-Text PDF
February 2021

Characterization of an NDM-5-producing hypervirulent sequence type 65 clone from a lung transplant recipient.

Emerg Microbes Infect 2021 Dec;10(1):396-399

Laboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Diseases, Beijing, People's Republic of China.

The emergence of New Delhi Metallo-β-lactamase (NDM)-producing has aroused critical concern worldwide. Herein, we reported the first emergence of NDM-5-producing isolates in a 68-year-old lung transplant recipient, who died of septic shock 13 days after surgery. The strain KP22937 isolated from the bloodstream of the patient was analyzed for phenotypes and genotypes. Read More

View Article and Full-Text PDF
December 2021

Identification and characterization of a spreadable IncI1 plasmid harbouring a bla gene in an Italian human isolate of Salmonella serovar Napoli.

Plasmid 2021 Mar 11;114:102566. Epub 2021 Feb 11.

OIE and National Reference Laboratory for Salmonellosis, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro (PD), Italy.

Salmonella enterica subsp. enterica serovar Napoli (S. Napoli) ranks among the top serovars causing human infections in Italy, although not common in other European countries. Read More

View Article and Full-Text PDF

Oxygen concentration affects frequency and range of transconjugants for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1.

Biosci Biotechnol Biochem 2021 Mar;85(4):1005-1015

Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka, Japan.

The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. Read More

View Article and Full-Text PDF

Post-HSCT graft failure due to refractory human cytomegalovirus successfully treated with haploidentical donor-derived immunoglobulins and stem cell graft infusion: A case report.

Antiviral Res 2021 Apr 9;188:105024. Epub 2021 Feb 9.

Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy. Electronic address:

Background: Human cytomegalovirus (HCMV) remains an important cause of transplant-related morbidity and mortality. The incidence of HCMV recurrence in the donor seronegative (D-)/recipient seropositive (R+) group is significantly higher than in other serostatus combinations as a result of a lack of pre-existing HCMV-specific memory T-lymphocytes in the donor, coupled with the eradication of the recipient's cellular immunity due to the conditioning regimen.

Case Presentation: We describe the case of an 8-year-old βE-thalassemic girl from Bangladesh who was seropositive for human cytomegalovirus (HCMV) and underwent hematopoietic stem cell transplantation from a HLA-matched, unrelated, HCMV-seronegative donor. Read More

View Article and Full-Text PDF

Case Report: Management of a Multidrug-Resistant CMV-Strain in a Renal Transplant Recipient by High-Dose CMV-Specific Immunoglobulins, Modulation in Immunosuppression, and Induction of CMV-Specific Cellular Immunity.

Front Immunol 2020 25;11:623178. Epub 2021 Jan 25.

Transplant Nephrology/Department of Internal Medicine D, University Hospital Münster, Westphalian Wilhelm's University Münster, Münster, Germany.

The management of multidrug-resistant strains of cytomegalovirus after solid organ transplantation is challenging. This case report demonstrates the successful treatment of a multidrug-resistant strain of cytomegalovirus that may represent a valuable option for problematic cases. This report illustrates the emergence of a multidrug-resistant cytomegalovirus (CMV) UL54 mutant strain in a renal transplant recipient with severe lymphopenia and thrombocytopenia. Read More

View Article and Full-Text PDF
January 2021

A single strain of Bacteroides fragilis protects gut integrity and reduces GVHD.

JCI Insight 2021 Feb 8;6(3). Epub 2021 Feb 8.

Department of Microbiology and Immunology, Hollings Cancer Center, College of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA.

Graft-versus-host disease (GVHD) is a pathological process caused by an exaggerated donor lymphocyte response to host antigens after allogeneic hematopoietic cell transplantation (allo-HCT). Donor T cells undergo extensive clonal expansion and differentiation, which culminate in damage to recipient target organs. Damage to the gastrointestinal tract is a main contributor to morbidity and mortality. Read More

View Article and Full-Text PDF
February 2021

In-home dementia caregiving is associated with greater psychological burden and poorer mental health than out-of-home caregiving: a cross-sectional study.

Aging Ment Health 2021 Feb 8:1-7. Epub 2021 Feb 8.

School of Health Sciences, City, University of London, London, UK.

Introduction: Caregivers who live with a person with dementia who receives care, compared with those who live elsewhere, are often considered to experience greater levels of psychological and affective burden. The evidence for this is, however, only limited to studies employing small sample sizes and that failed to examine caregivers' psychological wellbeing. We address these issues in a large cohort of dementia caregivers. Read More

View Article and Full-Text PDF
February 2021

Klebsiella pneumoniae carbapenemase (KPC) in urinary infection isolates.

Arch Microbiol 2021 Jan 28. Epub 2021 Jan 28.

School of Medicine, University of Zagreb, Zagreb, Croatia.

Recently, emergence of carbapenem-resistance, in particular due to Klebsiella pneumoniae carbapenemase (KPC), was observed among K. pneumoniae causing urinary tract infections in Croatia. The aim of the study was to characterize, antimicrobial susceptibility, carbapenem resistance, virulence traits and plasmid types of the urinary KPC positive isolates of K. Read More

View Article and Full-Text PDF
January 2021

Comparison of Genetically Engineered Immunodeficient Animal Models for Nonclinical Testing of Stem Cell Therapies.

Pharmaceutics 2021 Jan 20;13(2). Epub 2021 Jan 20.

Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon 14662, Korea.

For the recovery or replacement of dysfunctional cells and tissue-the goal of stem cell research-successful engraftment of transplanted cells and tissues are essential events. The event is largely dependent on the immune rejection of the recipient; therefore, the immunogenic evaluation of candidate cells or tissues in immunodeficient animals is important. Understanding the immunodeficient system can provide insights into the generation and use of immunodeficient animal models, presenting a unique system to explore the capabilities of the innate immune system. Read More

View Article and Full-Text PDF
January 2021

Whole genome sequence of a New Delhi metallo-β-lactamase 1-producing Proteus mirabilis isolate SNYG35 from broiler chicken in China.

J Glob Antimicrob Resist 2021 Mar 18;24:266-269. Epub 2021 Jan 18.

College of Life Sciences, Sichuan University, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, China. Electronic address:

Objectives: The global spread of the New Delhi metallo-β-lactamase 1 (NDM-1) gene poses a significant challenge to worldwide public health. Here, we characterize the whole genome of NDM-1-producing Proteus mirabilis isolate SNYG35 of broiler chicken origin in China.

Methods: The genome of SNYG35 was sequenced using a PacBio RS II sequencing instrument and Illumina HiSeq platform. Read More

View Article and Full-Text PDF

Predictive Parameters of Decreased Left Ventricular Global Longitudinal Strain at 1 Month After Pediatric Heart Transplantation.

Pediatr Cardiol 2021 Jan 19. Epub 2021 Jan 19.

Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-Gil, Songpa-Gu, Seoul, 138-736, Republic of Korea.

Previous reports indicate that the decreased left ventricular global longitudinal strain (LVGLS) seen in the early postoperative period of pediatric heart transplant patients generally recovers over the course of 1-2 years. In this study, we investigate the predictive capacity of preoperative parameters on the LVGLS decline seen at 1 month post transplant. Forty-six transplant subjects with 2D echocardiographic images sufficient for speckle tracking echocardiography were enrolled. Read More

View Article and Full-Text PDF
January 2021

Editing the immune system in vivo in mice using CRISPR/Cas9 ribonucleoprotein (RNP)-mediated gene editing of transplanted hematopoietic stem cells.

Methods 2021 Jan 7. Epub 2021 Jan 7.

AbbVie, Cambridge Research Center, 200 Sidney Street Cambridge, Massachusetts 02139, USA.

CRISPR/Cas9-based genome editing has been widely used to evaluate target gene function in biomedical research. The CRISPR/Cas9 system can introduce gene knockout, knock-in and mutations with more ease than earlier generations of genome editing tools. Using CRISPR/Cas9-based genome editing, researchers have successfully modified the DNA of different immune components, including primary T cells, B cells, macrophages, and immune system progenitors, i. Read More

View Article and Full-Text PDF
January 2021

Rapid Increase in the IS-Mediated Gene in Isolates with IncP and IncX4 Plasmids and Co-Existing and Genes in a Swine Farm.

Pathogens 2021 Jan 3;10(1). Epub 2021 Jan 3.

Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China.

This paper aimed to investigate the molecular epidemiological features of the gene in isolates in a typical swine farm during 2014-2017. A total of 617 isolates were screened for the gene using PCR amplification. A susceptibility test, pulsed-field gel electrophoresis (PFGE), S1-PFGE, southern blotting hybridization, and the genetic context of the gene were all used for analyzing all -positive isolates. Read More

View Article and Full-Text PDF
January 2021

Cardiac Responses to Prenatal Resistance Exercise with and without the Valsalva Maneuver.

Med Sci Sports Exerc 2020 Dec 23;Publish Ahead of Print. Epub 2020 Dec 23.

Program for Pregnancy and Postpartum Health, Physical Activity and Diabetes Laboratory, Faculty of Kinesiology, Sport and Recreation, Women and Children's Health Research Institute, Alberta Diabetes Institute, University of Alberta, Edmonton, Alberta, Canada Neurovascular Health Lab, Faculty of Kinesiology, Sport, and Recreation, Neuroscience and Mental Health Institute, Women and Children's Health Research Institute, Alberta Diabetes Institute, University of Alberta, Canada.

Purpose: Exercise guidelines recommend incorporating resistance exercise (RE) into a regular aerobic training program during pregnancy. Yet, few women do so due to uncertainties about the safety of prenatal RE, particularly regarding the Valsalva maneuver (VM). The aim of this study was to determine the acute cardiovascular responses to prenatal RE at different intensities, with and without VM. Read More

View Article and Full-Text PDF
December 2020

Whole-genome sequencing of strains of Vibrio spp. from China reveals different genetic contexts of blaCTX-M-14 among diverse lineages.

J Antimicrob Chemother 2021 Mar;76(4):950-956

Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong.

Objectives: To investigate the prevalence and genetic contexts of the blaCTX-M-14 gene harboured by foodborne isolates of Vibrio spp. in China.

Methods: A total of 1856 Vibrio spp. Read More

View Article and Full-Text PDF