2,232 results match your criteria recipient chimeric

Developmental potential of cryopreserved gonadal germ cells from 7-day-old chick embryos recovered using the PBS(-) method.

Br Poult Sci 2021 Jul 28. Epub 2021 Jul 28.

Faculty of Life and Environmental Sciences, University of Tsukuba, Ten-noh Dai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.

A series of experiments were conducted to examine the developmental potential of cryopreserved gonadal germ cells (GGCs) recovered from both males and females on embryo day 7 (7 d-GGCs) using the PBS(-) method. Germline chimeras were produced by transferring 200 frozen/unfrozen 7 d-GGCs recovered from female/male Rhode Island Red (RIR) embryos into the dorsal aorta of 2-day-old female and male white leghorn (WL) embryos.Germ-cell recipient embryos were hatched and raised to sexual maturity and progeny testing was conducted by mating with RIR of the opposite sex. Read More

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Lethal hyperammonemia in a CAR-T cell recipient due to pneumonia: a case report of a unique severe complication.

BMJ Case Rep 2021 Jul 8;14(7). Epub 2021 Jul 8.

Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Minnesota Medical School Twin Cities, Minneapolis, Minnesota, USA.

We report the first incidence of infection causing lethal hyperammonemia in a chimeric receptor antigen T cell (CAR-T) recipient. A 53-year-old woman, after receiving CAR-T therapy, suffered sepsis and encephalopathy. She was found to have hyperammonemia up to 643 µmol/L. Read More

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Burkholderia multivorans requires species-specific GltJK for entry of a contact-dependent growth inhibition system protein.

Mol Microbiol 2021 Jul 8. Epub 2021 Jul 8.

Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA.

Interbacterial antagonism and communication are driving forces behind microbial community development. In many Gram-negative bacteria, contact-dependent growth inhibition (CDI) systems contribute to these microbial interactions. CDI systems deliver the toxic C-terminus of a large surface exposed protein to the cytoplasm of neighboring bacteria upon cell-contact. Read More

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Clonal hematopoiesis and its emerging effects on cellular therapies.

Leukemia 2021 Jul 2. Epub 2021 Jul 2.

Medizinische Klinik und Poliklinik 1, Universitätsklinikum Carl Gustav Carus an der TU Dresden, Dresden, Germany.

The accumulation of somatic mutations in hematopoietic stem cells during aging, leading to clonal expansion, is linked to a higher risk of cardiovascular mortality and hematologic malignancies. Clinically, clonal hematopoiesis is associated with a pro-inflammatory phenotype of hematopoietic cells and their progeny, inflammatory conditions and a poor outcome for patients with hematologic neoplasms and solid tumors. Here, we review the relevance and complications of clonal hematopoiesis for the treatment of hematologic malignancies with cell therapeutic approaches. Read More

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Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.

STAR Protoc 2021 Jun 15;2(2):100494. Epub 2021 Jun 15.

Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

Interspecies chimerism is a useful tool to study interactions between cells of different genetic makeup in order to elucidate the mechanisms underlying non-cell-autonomous processes, including evolutionary events. However, generating interspecies chimeras with high efficiency and chimerism level remains challenging. Here, we describe a protocol for generating chimeras between mouse and rat. Read More

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Reconstruction of Large Area of Deep Wound in the Foot and Ankle with Chimeric Anterolateral Thigh Perforator Flap.

Orthop Surg 2021 Jul 17;13(5):1609-1617. Epub 2021 Jun 17.

Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Objective: To evaluate the clinical application and surgical efficacy of the chimeric perforator flap pedicled with the descending branch of the lateral circumflex femoral artery and the lateral thigh muscle flap for the reconstruction of the large area of deep wound in foot and ankle.

Methods: Clinical data of 32 cases who underwent chimeric anterolateral thigh perforator flap to repair the large area of deep wound of the foot and ankle from January 2015 to December 2018 were retrospectively analyzed. The sizes of the defects ranged from 18 cm × 10 cm to 35 cm × 20 cm, with exposed tendon and bone and/or partial defects and necrosis, contaminations, accompanied by different degrees of infection. Read More

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Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells.

Nat Commun 2021 06 11;12(1):3568. Epub 2021 Jun 11.

Division of Stem Cell Biology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Bone marrow (BM) chimeric mice are a valuable tool in the field of immunology, with the genetic manipulation of donor cells widely used to study gene function under physiological and pathological settings. To date, however, BM chimera protocols require myeloablative conditioning of recipient mice, which dramatically alters steady-state hematopoiesis. Additionally, most protocols use fluorescence-activated cell sorting (FACS) of hematopoietic stem/progenitor cells (HSPCs) for ex vivo genetic manipulation. Read More

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[Regulation of RNA Binding Protein Mbnl1 on Development of Mouse Embryonic Hematopoietic Stem Cells].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):924-930

School of Medicine, Jinan University, Guangzhou 510000, Guangdong Province, China,E-mail:

Objective: To analyze the dynamic molecular expression characteristics of single cell RNA binding proteins (RBPs) in the development of mouse embryonic hematopoitic stem cells (HSCs), and obtain the functional research target RNA splicing factor--Mbnl1, to clarify the function of Mbnl1 involved in regulating mouse embryonic HSC development.

Methods: Bioinformatics was used to analyze the single-cell transcriptome data of mouse embryos during HSC development, and the single-cell RBP dynamic molecular expression maps in HSC development was obtained. Mbnl1 was obtained by combining differential analysis and literature research screening. Read More

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CD40 Activity on Mesenchymal Cells Negatively Regulates OX40L to Maintain Bone Marrow Immune Homeostasis Under Stress Conditions.

Front Immunol 2021 18;12:662048. Epub 2021 May 18.

Department of Research, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy.

Background: Within the bone marrow (BM), mature T cells are maintained under homeostatic conditions to facilitate proper hematopoietic development. This homeostasis depends upon a peculiar elevated frequency of regulatory T cells (Tregs) and immune regulatory activities from BM-mesenchymal stem cells (BM-MSCs). In response to BM transplantation (BMT), the conditioning regimen exposes the BM to a dramatic induction of inflammatory cytokines and causes an unbalanced T-effector (Teff) and Treg ratio. Read More

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An extended superficial circumflex iliac artery perforator flap transfer for a relatively-small breast reconstruction after total mastectomy.

Microsurgery 2021 Jun 2. Epub 2021 Jun 2.

Department of Plastic and Reconstructive Surgery, National Center for Global Health and Medicine (NCGM), Tokyo, Japan.

Superficial circumflex iliac artery (SCIA) perforator (SCIP) flap has been applied in various reconstructions. Unlike traditional groin flap, SCIP flap has a longer pedicle and can be used as a chimeric flap for complex reconstruction. By utilizing both the superficial and the deep branches of the SCIA, a SCIP flap can be raised as an extended large bulky flap. Read More

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Graft-versus-host disease risk after chimeric antigen receptor T-cell therapy: the diametric opposition of T cells.

Br J Haematol 2021 May 25. Epub 2021 May 25.

Division of Hematological Malignancies and Bone Marrow Transplantation, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.

Chimeric antigen receptor (CAR) T-cell therapy has brought a paradigm shift in the management of haematological malignancies and has opened novel avenues of investigational therapeutic strategies. Given these encouraging responses, it has become imperative to understand the full spectrum of biology and potential toxicities that can arise from these novel agents, as well as those under investigation. With the increasing use of CAR T-cell therapy for relapse following allogeneic haematopoietic cell transplantation (HCT) and the imminence of allogeneic CAR T cells, risks from T cell-based therapy, such as the previously well-recognised graft-versus-host disease (GVHD), have gained prominence and warrant explanation. Read More

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Running the full human developmental clock in interspecies chimeras using alternative human stem cells with expanded embryonic potential.

NPJ Regen Med 2021 May 17;6(1):25. Epub 2021 May 17.

Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Human pluripotent stem cells (hPSCs) can generate specialized cell lineages that have great potential for regenerative therapies and disease modeling. However, the developmental stage of the lineages generated from conventional hPSC cultures in vitro are embryonic in phenotype, and may not possess the cellular maturity necessary for corrective regenerative function in vivo in adult recipients. Here, we present the scientific evidence for how adult human tissues could generate human-animal interspecific chimeras to solve this problem. Read More

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The chimeric medial sural artery perforator flap as the ideal for partial tongue reconstruction: A case series and technical refinements.

J Plast Reconstr Aesthet Surg 2021 Mar 29. Epub 2021 Mar 29.

Section of Plastic Surgery, The University of Chicago Medicine & Biological Sciences, Chicago, IL, USA.

Background: The medial sural artery perforator free flap (MSAP) has gained increasing popularity in head and neck reconstruction. Its slightly bulkier nature than the radial forearm flap, combined with negligible donor site morbidity, makes it an ideal candidate for the reconstruction of partial glossectomy defects. The ability to harvest the MSAP as a chimeric flap with a portion of the medial gastrocnemius muscle gives it greater flexibility in soft tissue reconstruction. Read More

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Derivation and characterization of putative embryonic stem cells isolated from blastoderms of Taiwan Country chicken for the production of chimeric chickens.

Anim Biotechnol 2020 Dec 2:1-10. Epub 2020 Dec 2.

Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.

The conservation of Taiwan Country chicken (TCC) is important due to concerns for the local breed's adaptability to the area and disease resistance. Furthermore, the genetic resource base of native chickens can be used to improve egg and meat production efficiency in commercial TCC. As the embryonic stem cells (ESCs) hold great potential for regenerative medicine and species conservation, the aims of this study were to isolate and characterize ESCs of TCC. Read More

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December 2020

Donor Recipient Chimeric Cells Induce Chimerism and Extend Survival of Vascularized Composite Allografts.

Arch Immunol Ther Exp (Warsz) 2021 May 10;69(1):13. Epub 2021 May 10.

Department of Orthopaedics, University of Illinois At Chicago, Molecular Biology Research Building, 900 S. Ashland Ave. Room# 3356, Chicago, IL, 60607, USA.

This study evaluated the efficacy of donor recipient chimeric cell (DRCC) therapy created by fusion of donor and recipient derived bone marrow cells (BMC) in chimerism and tolerance induction in a rat vascularized composite allograft (VCA) model. Twenty-four VCA (groin flaps) from MHC-mismatched ACI (RT1) donors were transplanted to Lewis (RT1) recipients. Rats were randomly divided into (n = 6/group): Group 1-untreated controls, Groups 2-7-day immunosuppression controls, Group 3-DRCC, and Group 4-DRCC with 7-day anti-αβTCR monoclonal antibody and cyclosporine A protocol. Read More

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Development of Donor Recipient Chimeric Cells of bone marrow origin as a novel approach for tolerance induction in transplantation.

Stem Cell Investig 2021 19;8. Epub 2021 Apr 19.

Department of Orthopaedics, University of Illinois at Chicago, Chicago, IL, USA.

Background: Cell therapies and chimerism-based strategies are currently the most successful approach for tolerance induction in transplantation. This study aimed to establish and characterize novel Donor Recipient Chimeric Ccell (DRCC) therapy of bone marrow (BM) origin presenting donor-recipient phenotype to support tolerance induction.

Methods: fusions of fully MHC-mismatched BM cells from ACI (RT1) and Lewis (RT1) rats were performed using polyethylene-glycol (PEG). Read More

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[Psychological issues of allogeneic bone marrow transplantation in adults].

Aurélie Berr

Soins 2021 Apr;66(854):43-45

Institut de cancérologie Strasbourg-Europe, Pôle évaluation, soins de support et accompagnement, 17 rue Albert-Calmette, 67200 Strasbourg, France. Electronic address:

A bone marrow transplant is often the only hope for a cure for some patients with blood disease. Beyond its medical aspects, it underlies psychological issues for both the recipient and the donor. The transplant brings together the issues of donation, debt, the family romance and the incorporation of a "foreign body", evoking the classic notion of chimera. Read More

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Serum sickness induced by alemtuzumab in a kidney-pancreas transplant recipient.

Clin Nephrol 2021 Jul;96(1):47-50

Serum sickness is an immune-complex-mediated hypersensitivity reaction that was first noted in the early 1900s in patients receiving heterologous antisera, such as horse antitetanus or antidiphtheria serum. This condition is primarily self-limited; however, in its acute state, it can cause severe symptoms of fever, rash, polyarthritis, or polyarthralgias. In solid organ transplantation, this condition is frequently reported in association with the use of rabbit anti-thymocyte globulin and chimeric murine monoclonal antibodies such as rituximab. Read More

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Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia.

Cell Death Dis 2021 05 1;12(5):435. Epub 2021 May 1.

Laboratory of Molecular Cardiology, IRCCS Policlinico San Donato, 20097, San Donato Milanese, Milan, Italy.

Hypoxia-induced miR-210 is a crucial component of the tissue response to ischemia, stimulating angiogenesis and improving tissue regeneration. Previous analysis of miR-210 impact on the transcriptome in a mouse model of hindlimb ischemia showed that miR-210 regulated not only vascular regeneration functions, but also inflammation. To investigate this event, doxycycline-inducible miR-210 transgenic mice (Tg-210) and anti-miR-210 LNA-oligonucleotides were used. Read More

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In vivo enrichment of busulfan-resistant germ cells for efficient production of transgenic avian models.

Sci Rep 2021 Apr 28;11(1):9127. Epub 2021 Apr 28.

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Korea.

Most transgenic animals are generated using a genome-modified stem cell system and genome modification directly in embryos. Although this system is well-established in the development of transgenic animals, donor cell-derived transgenic animal production is inefficient in some cases. Especially in avian models such as chickens, the efficiency of transgenic animal production through primordial germ cells (PGCs) is highly variable compared with embryonic manipulation of mammalian species. Read More

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Efficiency comparison of B6(Cg)-Tyr /J and C57BL/6NTac embryos as hosts for the generation of knockout mice.

Transgenic Res 2021 06 12;30(3):275-281. Epub 2021 Apr 12.

CAM-SU Genomic Resource Center, Soochow University, Suzhou, 215123, Jiang Su, China.

Careful selection of the host embryo is critical to the efficient production of knockout (KO) mice when injecting mouse embryonic stem (mES) cells into blastocysts. B6(Cg)-Tyr/J (B6 albino) and C57BL/6NTac (B6NTac) strains of mice are widely used to produce host blastocysts for such procedures. Here, we tested these two strains to identify an appropriate match for modified agouti C57BL/6N (JM8A3. Read More

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Generation of Murine Bone Marrow and Fetal Liver Chimeras.

Curr Protoc 2021 Apr;1(4):e79

Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, Australia.

The generation of radiation chimeras allows researchers to substitute the hematopoietic system of a mouse with that of one or more donors. A suspension of hematopoietic stem cells (HSCs) is prepared from the bone marrow (BM) or the fetal liver (FL) of a donor mouse and adoptively transferred into an irradiated recipient. Within days, the donor's HSCs will engraft, and their progeny will quickly replace the blood cells of the recipient. Read More

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The CD28-Transmembrane Domain Mediates Chimeric Antigen Receptor Heterodimerization With CD28.

Front Immunol 2021 23;12:639818. Epub 2021 Mar 23.

Department of Surgery, University of California, San Francisco, San Francisco, CA, United States.

Anti-CD19 chimeric antigen receptor (CD19-CAR)-engineered T cells are approved therapeutics for malignancies. The impact of the hinge domain (HD) and the transmembrane domain (TMD) between the extracellular antigen-targeting CARs and the intracellular signaling modalities of CARs has not been systemically studied. In this study, a series of 19-CARs differing only by their HD (CD8, CD28, or IgG) and TMD (CD8 or CD28) was generated. Read More

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Proceedings From the First International Workshop at Sidra Medicine: "Engineered Immune Cells in Cancer Immunotherapy (EICCI): From Discovery to Off-the-Shelf Development", 15-16 February 2019, Doha, Qatar.

Front Immunol 2020 14;11:589381. Epub 2021 Jan 14.

Research Department, Sidra Medicine, Doha, Qatar.

The progress in the isolation and characterization of tumor antigen (TA)-specific T lymphocytes and in the genetic modification of immune cells allowed the clinical development of adoptive cell therapy (ACT). Several clinical studies highlighted the striking clinical activity of T cells engineered to express either Chimeric Antigen (CAR) or T Cell (TCR) Receptors to target molecularly defined antigens expressed on tumor cells. The breakthrough of immunotherapy is represented by the approval of CAR-T cells specific for advanced or refractory CD19 B cell malignancies by both the Food and Drug Administration (FDA) and the European Medicinal Agency (EMA). Read More

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Endothelial TLR4 Expression Mediates Vaso-Occlusive Crisis in Sickle Cell Disease.

Front Immunol 2020 19;11:613278. Epub 2021 Jan 19.

Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota, Minneapolis, MN, United States.

Heme, released from red blood cells in sickle cell disease (SCD), interacts with toll-like receptor 4 (TLR4) to activate NF-κB leading to the production of cytokines and adhesion molecules which promote inflammation, pain, and vaso-occlusion. In SCD, TLR4 inhibition has been shown to modulate heme-induced microvascular stasis and lung injury. We sought to delineate the role of endothelial verses hematopoietic TLR4 in SCD by developing a TLR4 null transgenic sickle mouse. Read More

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Novel Design of the Chimeric Deep Inferior Epigastric Artery Perforator Flap that Provides for Three-Dimensional Reconstruction of Composite Tissue Defects of the Heel in Children.

Orthop Surg 2021 Feb 15;13(1):216-224. Epub 2021 Jan 15.

Department of Hand and Microsurgery, Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, China.

Objective: The aim of the present study was to report a novel design of the chimeric deep inferior epigastric artery perforator flap (DIEP) to achieve dead space filling, Achilles tendon bridging, and skin resurfacing simultaneously with minimal donor-site morbidity.

Methods: From September 2012 to May 2016, a retrospective study was carried out on six pediatric patients with composite soft tissue defects of the heel that were repaired with the chimeric DIEP flap. The chimeric flap design included a flap of the anterior sheath of the rectus, a block of rectus muscle, and a large skin paddle. Read More

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February 2021

Short-term therapy with anti-ICAM-1 monoclonal antibody induced long-term liver allograft survival in nonhuman primates.

Am J Transplant 2021 Jan 10. Epub 2021 Jan 10.

Department of Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea.

Tolerance induction remains challenging following liver transplantation and the long-term use of immunosuppressants, especially calcineurin inhibitors, leads to serious complications. We aimed to test an alternative immunosuppressant, a chimeric anti-ICAM-1 monoclonal antibody, MD-3, for improving the outcomes of liver transplantation. We used a rhesus macaque liver transplantation model and monkeys were divided into three groups: no immunosuppression (n = 2), conventional immunosuppression (n = 4), and MD-3 (n = 5). Read More

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January 2021

Pathology of macrophage activation syndrome in humanized NSGS mice.

Res Vet Sci 2021 Jan 16;134:137-146. Epub 2020 Dec 16.

Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA.

"Humanized" immunodeficient mice generated via the transplantation of CD34+ human hematopoietic stem cells (hHSC) are an important preclinical model system. The triple transgenic NOD.Cg-PrkdcIl2rg Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ (NSGS) mouse line is increasingly used as recipient for CD34+ hHSC engraftment. Read More

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January 2021