82,097 results match your criteria real-time quantitative


MiR-339 is a potential biomarker of coronary heart disease to aggravate oxidative stress through Nrf2/FOXO3 targeting Sirt2.

Ann Palliat Med 2021 Mar;10(3):2596-2609

Department of Cardiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Background: MicroRNA (miRNA) is a class of small-molecule RNA that can regulate gene expression at post-transcription level. It is involved in the genesis and development of multiple diseases. The aim of this paper was to explore the mechanisms of miR-339 in coronary heart disease (CHD). Read More

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Towards quantitative and intuitive percutaneous tumor puncture via augmented virtual reality.

Comput Med Imaging Graph 2021 Mar 31;90:101905. Epub 2021 Mar 31.

Department of Computer Science and Engineering, Chinese University of Hong Kong, Hong Kong SAR, China.

In recent years, the radiofrequency ablation (RFA) therapy has become a widely accepted minimal invasive treatment for liver tumor patients. However, it is challenging for doctors to precisely and efficiently perform the percutaneous tumor punctures under free-breathing conditions. This is because the traditional RFA is based on the 2D CT Image information, the missing spatial and dynamic information is dependent on surgeons' experience. Read More

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Bone marrow-derived mesenchymal stem cells attenuate silica-induced pulmonary fibrosis by inhibiting apoptosis and pyroptosis but not autophagy in rats.

Ecotoxicol Environ Saf 2021 Apr 10;216:112181. Epub 2021 Apr 10.

Department of Occupational Health and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address:

This study aimed to investigate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on silica-induced lung fibrosis in a rat model. Thirty SD rats were randomly divided into three groups: control group, silica group, and BMSC group (n = 10 rats per group). BMSCs were injected successively into rats on the 14th, 28th, and 42nd days after silica exposure. Read More

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Prevalence and molecular characterization of C. pecorum detected in Swiss fattening pigs.

Vet Microbiol 2021 Mar 31;256:109062. Epub 2021 Mar 31.

Institute of Veterinary Pathology, Vetsuisse-Faculty University Zurich, Zurich, 8057, Switzerland; Center for Clinical Studies, Vetsuisse-Faculty, University of Zurich, Zurich, 8057, Switzerland.

Chlamydia (C.) pecorum, an obligate intracellular bacterial species commonly found in ruminants, can also occur in pigs. However, its significance as a potential porcine pathogen, or commensal, is still unclear. Read More

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Characterization of mandarin fish (Siniperca chuatsi) IL-6 and IL-6 signal transducer and the association between their SNPs and resistance to ISKNV disease.

Fish Shellfish Immunol 2021 Apr 10. Epub 2021 Apr 10.

Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou, 510380, China.

In fish, interleukin-6 (IL-6) is a very important immune-regulatory cytokine that plays a polyfunctional role in inflammation, metabolism, regeneration, and neural processes. IL-6 signal transducer (IL-6ST) is a specific receptor for IL-6 and expressed mainly in immune cells and hepatocytes. In this study, the complete cDNA and genomic DNA sequences of mandarin fish (Siniperca chuatsi) IL-6 and IL-6ST genes were identified and analyzed. Read More

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Development and Application of SYBR Green Ⅰ Real-time Quantitative Reverse Transcription PCR Assay for Detection of Swine Getah Virus.

Mol Cell Probes 2021 Apr 10:101730. Epub 2021 Apr 10.

College of Veterinary Medicine, Henan Agricultural University. Electronic address:

Getah virus (GETV), a mosquito-borne virus belonging to the Alphavirus genus of family Togaviridae, has become increasingly problematic, which poses a huge threat to the safety of animals and public health. In order to detect GETV quickly and accurately, we have developed a SYBR Green I real-time quantitative reverse transcription PCR (RT-qPCR) assay for GETV with the detection limit of 66 copies/μL, excellent correlation coefficient (R) of 0.9975, and amplification efficiency (E) of 98. Read More

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Synergistic effects of green tea extract and Paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines.

Gene 2021 Apr 10:145638. Epub 2021 Apr 10.

Department of Medical Radiation, Faculty of Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran. Electronic address:

Background: Green tea is a natural compound with anti-neoplastic properties. Paclitaxel (PTX) is a natural anti-tumor medication used to manage patients with advanced ovarian cancer. This manuscript evaluated the cytotoxic effects of green tea extract combined with PTX drug in two human ovarian cancer cell lines (p53-negative cell line, SKOV-3; and mutant type p53 cell line, OVCAR-3) and underlying mechanisms. Read More

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Genome-wide characterization and expression profiling of the PDR gene family in N. tabacum.

Gene 2021 Apr 10:145637. Epub 2021 Apr 10.

China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China. Electronic address:

The pleiotropic drug resistance (PDR) proteins of the ATP-binding cassette (ABC) family play essential roles in physiological processes and have been characterized in many plant species. However, no comprehensive investigation of tobacco (Nicotiana tabacum), an important economic crop and a useful model plant for scientific research, has been presented. We identified 32 PDR genes in the tobacco genome and explored their domain organization, chromosomal distribution and evolution, promoter cis-elements, and expression profiles. Read More

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Anti-thrombotic activity of phenolic acids obtained from Salvia miltiorrhiza f. alba in TNF-α-stimulated endothelial cells via the NF-κB/JNK/p38 MAPK signaling pathway.

Arch Pharm Res 2021 Apr 13. Epub 2021 Apr 13.

Department of Natural Product Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, No. 44 West Wenhua Road, Jinan, 250012, People's Republic of China.

Over the past 100 years, Salvia miltiorrhiza f. alba (Lamiaceae) (RSMA) roots have been used to cure thromboangiitis obliterans (TAO) in local clinics. This study aimed to confirm the anti-thrombotic efficacy of 12 phenolic acids obtained from RSMA and to clarify the possible underlying mechanisms. Read More

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LncRNA LOC729178 acts as a sponge of miR-144-3p to mitigate cigarette smoke extract-induced inflammatory injury via regulating PHLPP2 in 16HBE cells.

J Mol Histol 2021 Apr 13. Epub 2021 Apr 13.

First People's Hospital of Jining City Affiliated to Jining Medical College, Jining City, Shandong Province, China.

Chronic obstructive pulmonary disease (COPD) is an inflammatory respiratory disease. Long non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of COPD. In the present study, we set to investigate the role and mechanism of LOC729178 on cigarette smoke extract (CSE)-induced inflammatory damage in 16HBE cells. Read More

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Circulating miR-449a predicts survival outcome for colorectal cancer following curative resection: An observational study.

Medicine (Baltimore) 2021 Apr;100(15):e25022

Department of VIP Medical Services, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.

Abstract: Previous studies showed that microRNA (miR)-449a may function as a tumor suppressor. However, the expression pattern and value of circulating miR-449a in colorectal cancer (CRC) remain unclear. Therefore, the purpose of this study was to measure circulating miR-449a level of CRC patients and evaluate its value for predicting prognosis. Read More

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Diagnostic testing for feline panleukopenia in a shelter setting: a prospective, observational study.

J Feline Med Surg 2021 Apr 13:1098612X211005301. Epub 2021 Apr 13.

Ontario Veterinary College, Guelph, Canada.

Objectives: The aim of this study was to optimize the diagnosis of feline panleukopenia virus (FPV) in a shelter setting by: (1) comparing the results of the canine parvovirus IDEXX SNAP Parvo (SNAP) point-of-care ELISA with a commercial FPV quantitative real-time PCR (qPCR) test; (2) assessing whether vomit and anal/rectal swabs could be used for early diagnosis; and (3) clarifying the interpretation of weak-positive SNAP test results.

Methods: The study included shelter cats and kittens with incomplete or unknown vaccination history that had clinical signs suspicious for feline panleukopenia and fecal SNAP and PCR tests performed within 24 h of onset. Feces, anal/rectal swabs and vomit were tested using SNAP and PCR, with fecal PCR utilized as the reference standard. Read More

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High glucose promotes the aging of human dental pulp cells through Wnt/beta-catenin signaling.

Dent Med Probl 2021 Jan-Mar;58(1):39-46

Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Iran.

Background: Diabetes is one of the most common metabolic diseases that disrupt the functioning of different body organs, including oral tissue. Some diabetic complications remain even after the control of the hyperglycemic condition. The adverse effect of hyperglycemia on the pulp structure and function has been shown previously. Read More

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