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Long non-coding RNA LINC01003 suppresses the development of multiple myeloma by targeting miR-33a-5p/PIM1 axis.

Leuk Res 2021 Mar 31;106:106565. Epub 2021 Mar 31.

Department of Hematology, The First Affiliated Hospital of Anhui Medical University, No. 218, Jixi Road, Shushan District, Hefei City, Anhui Province, 230032, China. Electronic address:

Background: Numerous long non-coding RNAs (lncRNAs) are reported to affect the progression of multiple myeloma (MM). This study is aimed to explore the role and downstream mechanism of lncRNA LINC01003 in MM.

Materials And Methods: Xenograft tumor assay was used to assess the function of LINC01003 in MM in vivo. Read More

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Differences in growth of Trypanoplasma borreli in carp serum is dependent on transferrin genotype.

Fish Shellfish Immunol 2021 Apr 14. Epub 2021 Apr 14.

Polish Academy of Sciences, Institute of Ichthyobiology and Aquaculture in Gołysz, Zaborze, 43-520, Chybie, Poland. Electronic address:

Kinetoplastid parasites require transferrin (Tf), being the main source of iron, for growth and multiplication. This group of parasites developed a unique receptor-mediated system for acquiring host Tf which bears no structural homology with the host transferrin receptor. Trypanoplasma borreli, a blood parasite of common carp, probably uses a similar mechanism to sequester iron from host transferrin. Read More

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MOL-PCR and xMAP technology - a multiplex system for fast detection of food- and water-borne viruses.

J Mol Diagn 2021 Apr 14. Epub 2021 Apr 14.

Department of Animal Origin Food and Gastronomic Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1946/1, CZ61242 Brno, Czech Republic.

Viruses are common causes of food- and water-borne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy or real-time polymerase chain reaction. Since recent technological advancements in detection methods are focused on fast and robust analysis, a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination, was utilized. Read More

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Melatonin indirectly decreases gastric cancer cell proliferation and invasion via effects on cancer-associated fibroblasts.

Life Sci 2021 Apr 14:119497. Epub 2021 Apr 14.

Division of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, PR China. Electronic address:

Aims: Gastric cancer is a malignant tumor with a poor prognosis, and the interaction between tumor cells and cancer-associated fibroblasts (CAFs) further contributes to progression and treatment failure. Recent studies have revealed the potential value of melatonin in cancer therapy, but its role in gastric cancer and CAFs requires further exploration.

Main Methods: CAFs were isolated using the tissue block method. Read More

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GAS5 regulates viability and apoptosis in TGF-β1-stimulated bronchial epithelial cells by regulating miR-217/HDAC4 axis.

Genes Genomics 2021 Apr 17. Epub 2021 Apr 17.

Department of Pediatrics, Heping Hospital Affiliated to Changzhi Medical College, No. 110, Yanan Sourth Road, Changzhi, 046000, Shanxi Province, China.

Background: Asthma is a serious respiratory disease that affects the physical and mental health of children. Airway epithelial apoptosis concomitantly mediated by transforming growth factor-β1 (TGF-β1) is a crucial component of asthma pathogenesis. LncRNA growth Arrest Specific 5 (GAS5), microRNA-217 (miR-217) and Histone deacetylase 4 (HDAC4) shown a close relationship with TGF-β1-induced injury of airway epithelial. Read More

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RNA-Seq Transcriptome Analysis of the Liver and Brain of the Black Carp (Mylopharyngodon piceus) During Fasting.

Mar Biotechnol (NY) 2021 Apr 17. Epub 2021 Apr 17.

College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, P. R. China.

The black carp (Mylopharyngodon piceus) is an important carnivorous freshwater-cultured species. To understand the molecular basis underlying the response of black carp to fasting, we used RNA-Seq to analyze the liver and brain transcriptome of fasting fish. Annotation to the NCBI database identified 66,609 unigenes, of which 22,841 were classified into the Gene Ontology database and 15,925 were identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Read More

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Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1 mediated deacetylation of GLI1 and hepatic stellate cell activation.

Br J Pharmacol 2021 Apr 17. Epub 2021 Apr 17.

State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Bioactive Natural Product Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.

Background And Purpose: Liver fibrosis is one of the leading causes of morbidity and mortality worldwide of which no acceptable therapy exists. Accumulating evidence supports that glioma-associated oncogene homologue 1 (GLI1) is a potentially important therapeutic target for liver fibrosis. This study investigates the antifibrotic activities and potential mechanisms of Physalin B (PB), a natural Solanaceae compound. Read More

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Evaluation of the relationship between laboratory parameters and pulmonary function tests in COVID-19 patients.

Int J Clin Pract 2021 Apr 17:e14237. Epub 2021 Apr 17.

Depertmant of Infection Diseases and Clinical Microbiology, Health Sciences University Erzurum Regional Education and Research Hospital, Yakutiye Erzurum, Turkey.

Objective: The novel coronavirus SARS-CoV-2 (COVID-19) rapidly escalated from its origin in an animal market in Wuhan, China in December 2019 to a global pandemic, and the lungs are the most frequently affected organ. The aim of this study was to investigate the relationship between pulmonary function test parameters and laboratory parameters in COVID-19.

Method: A total of 60 patients who were admitted to the chest diseases department and intensive care unit of our hospital and were diagnosed with COVID-19 by real-time PCR analysis of nasopharyngeal swabs were evaluated. Read More

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Limited Impact of 6-Mercaptopurine on Inflammation-Induced Chemokines Expression Profile in Primary Cultures of Enteric Nervous System.

Neurochem Res 2021 Apr 16. Epub 2021 Apr 16.

Institute of Anatomy, Kiel University, Kiel, Germany.

Increasing evidences indicate that the enteric nervous system (ENS) and enteric glial cells (EGC) play important regulatory roles in intestinal inflammation. Mercaptopurine (6-MP) is a cytostatic compound clinically used for the treatment of inflammatory bowel diseases (IBD), such as ulcerative colitis and Crohn's disease. However, potential impacts of 6-MP on ENS response to inflammation have not been evaluated yet. Read More

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Cloprostenol, a synthetic analogue of prostaglandin F2α induces functional regression in cultured luteal cells of felids†.

Biol Reprod 2021 Apr 16. Epub 2021 Apr 16.

Leibniz Institute for Zoo and Wildlife Research, Department of Reproduction Biology, 10315 Berlin, Germany.

In the present study, we investigated the effect of the synthetic analogue of prostaglandin F2α (PGF2α)-cloprostenol-on cultured steroidogenic luteal cells of selected felid species over a two day culture period. The changes induced by cloprostenol were measured based on progesterone concentration and mRNA expression analysis of selected genes. Cloprostenol significantly reduced concentration of progesterone in cell culture medium of small luteal cells isolated from domestic cat corpora lutea (CL) at the development/maintenance stage (p < 0. Read More

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Multiple internal controls enhance reliability for PCR and real time PCR detection of Rathayibacter toxicus.

Sci Rep 2021 Apr 16;11(1):8365. Epub 2021 Apr 16.

Department of Plant Pathology, Great Plains Diagnostic Network, Kansas State University, 4024 Throckmorton Plant Sciences Center, Manhattan, KS, 66506-5502, USA.

Rathayibacter toxicus is a toxigenic bacterial plant pathogen indigenous to Australia and South Africa. A threat to livestock industries globally, the bacterium was designated a U.S. Read More

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The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification.

Sci Rep 2021 Apr 16;11(1):8402. Epub 2021 Apr 16.

Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, China.

The Chinese herbal formula TiaoGanYiPi (TGYP) showed effective against chronic hepatitis B (CHB) caused by hepatitis B virus (HBV) infection. Hence, we aimed to clarify the mechanisms and potential targets between TGYP and CHB. The active compounds and related putative targets of TGYP, and disease targets of CHB were obtained from the public databases. Read More

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Interleukin-35 regulates peripheral T cell activity in patients with Kawasaki disease.

Int Immunopharmacol 2021 Apr 13;96:107642. Epub 2021 Apr 13.

Department of General Internal Medicine, The Children's Hospital Affiliated to Xi'an Jiaotong University (Xi'an Children's Hospital), Xi'an, Shaanxi Province 710003, China. Electronic address:

Interleukin-35 (IL-35) regulates immune cell function in inflammation, infection, cancer, and autoimmune diseases. However, the modulatory activity of IL-35 exerted on T cells is not fully understood in Kawasaki disease. For this purpose, the present study included 28 patients with Kawasaki disease and 16 healthy controls. Read More

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Berberine, a potential prebiotic to indirectly promote Akkermansia growth through stimulating gut mucin secretion.

Biomed Pharmacother 2021 Apr 14;139:111595. Epub 2021 Apr 14.

Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. Electronic address:

Background: Akkermansia spp. plays important roles in maintenance of host health. Increasing evidence reveals that berberine (BBR) may exert its pharmacological effects via, at least partially, promotion of Akkermansia spp. Read More

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Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection.

Int J Infect Dis 2021 Apr 13. Epub 2021 Apr 13.

Department of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, Japan; Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated for diagnosis of several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. The aim of this study was to determine the diagnostic sensitivity and specificity of RT-LAMP compared to reverse transcription-quantitative polymerase chain reaction (RT-qPCR) over the disease course of COVID-19. Read More

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Corilagin alleviates hypertrophic scars via inhibiting the transforming growth factor (TGF)-β/Smad signal pathway.

Life Sci 2021 Apr 13:119483. Epub 2021 Apr 13.

Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Aims: Exploring the effects of corilagin on hypertrophic scar (HS) and its underlying mechanisms.

Main Methods: Human HS-derived fibroblasts (HSFs) were isolated and treated with corilagin. To investigate the effects of corilagin on HSFs, quantitative real time polymerase chain reaction (qRT-PCR), western blotting, wound healing, and immunofluorescence assays were performed. Read More

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A tumorigenic cell line derived from a hamster cholangiocarcinoma associated with Opisthorchis felineus liver fluke infection.

Life Sci 2021 Apr 13:119494. Epub 2021 Apr 13.

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG SB RAS), 10 Lavrentiev Ave., Novosibirsk 630090, Russia; Department of Natural Sciences, Novosibirsk State University, 2 Pirogova Str., Novosibirsk 630090, Russia.

Aims: The food-born trematode Opisthorchis felineus colonizes bile ducts of the liver of fish-eating mammals including humans. There is growing evidence that this liver fluke is a risk factor for cholangiocarcinoma (CCA). Cancer cell lines are necessary for drug screening and for identifying protein markers of CCA. Read More

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Molecular cloning and characterization of CD63 in common carp infected with koi herpesvirus.

Dev Comp Immunol 2021 Apr 13:104102. Epub 2021 Apr 13.

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China. Electronic address:

CD63 is a member of the four-transmembrane-domain protein superfamily and is the first characterized tetraspanin protein. In the present study, we cloned the common carp (Cyprinus Carpio) CD63 (ccCD63) sequence and found that the ccCD63 ORF contained 711 bp and encoded a protein of 236 amino acids. Homology analysis revealed that the complete ccCD63 sequence had 84. Read More

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Pancreastatin induces islet amyloid peptide aggregation in the pancreas, liver, and skeletal muscle: An implication for type 2 diabetes.

Int J Biol Macromol 2021 Apr 13. Epub 2021 Apr 13.

Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226031, India; Pharmacology Division, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow 226031, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, Uttar Pradesh, India. Electronic address:

Recent findings suggest that the accumulation of misfolded aggregates of islet amyloid peptide (IAPP) plays an essential role in pancreatic damage and type 2 diabetes (T2D). Pancreastatin (PST), a chromogranin derived peptide, instigates insulin resistance (IR) and promotes T2D. Here, we aimed to investigate whether PST induces IAPP aggregation in the pancreas, liver, and skeletal muscles. Read More

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Validation of a host blood transcriptomic biomarker for pulmonary tuberculosis in people living with HIV: a prospective diagnostic and prognostic accuracy study.

Lancet Glob Health 2021 Apr 13. Epub 2021 Apr 13.

South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town, Cape Town, South Africa. Electronic address:

Background: A rapid, blood-based triage test that allows targeted investigation for tuberculosis at the point of care could shorten the time to tuberculosis treatment and reduce mortality. We aimed to test the performance of a host blood transcriptomic signature (RISK11) in diagnosing tuberculosis and predicting progression to active pulmonary disease (prognosis) in people with HIV in a community setting.

Methods: In this prospective diagnostic and prognostic accuracy study, adults (aged 18-59 years) with HIV were recruited from five communities in South Africa. Read More

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SIRT2 and SIRT3 expression correlates with redox imbalance and advanced clinical stage in patients with multiple myeloma.

Clin Biochem 2021 Apr 13. Epub 2021 Apr 13.

Department of Clinical and Experimental Medicine, University of Messina, Messina Italy.

Objectives: Sirtuins comprise seven family elements (SIRT1-7) involved in various cell signalling pathways comprising cancer inhibition and tumorigenesis. The present study aims to evaluate SIRT2 and SIRT3 gene expression and potential redox reactions in patients with multiple myeloma (MM) at onset and its correlation with disease status, extent and presence of organ damage secondary to myeloma.

Design & Methods: Total RNA was extracted from 17 MM patients and 10 controls to assess gene expression using real-time PCR. Read More

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The Effect of Inhibition through DZNep on Epithelial-Mesenchymal Transition Mechanism.

Cell Reprogram 2021 Apr;23(2):139-148

Department of Biochemistry, Erciyes University School of Medicine, Kayseri, Turkey.

Although the molecular pathogenesis of hepatocellular carcinoma (HCC) is uncertain, it is known that the epithelial-mesenchymal transition (EMT) mechanism and epigenetic changes have an important role. This study was focused on evaluating the relationship of 3-Deazaneplanocin A (DZNep) with the EMT mechanism, which is a histone methyltransferase inhibitor on HCC and is also known as an enhancer of zeste homolog 2 (EZH2) inhibitor. Cell viability of HepG2 cells (HCC cell line) assessed for DZNep over 72 hours with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Read More

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M2a macrophage can rescue proliferation and gene expression of benign prostate hyperplasia epithelial and stroma cells from insulin-like growth factor 1 knockdown.

Prostate 2021 Apr 16. Epub 2021 Apr 16.

Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Background: Benign prostatic hyperplasia (BPH) is a common disease in elderly men and is often accompanied by chronic inflammation. Macrophages (several subtypes) are the main inflammatory cells that infiltrate the hyperplastic prostate and are found to secrete cytokines and growth factors. The current study aims to explore the effect of M2a macrophages on the development of BPH via insulin-like growth factor 1 (IGF-1). Read More

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Transcriptomic analysis of diapause-associated genes in Exorista civilis Rondani (Diptera:Tachinidae).

Arch Insect Biochem Physiol 2021 Apr 16:e21789. Epub 2021 Apr 16.

Research Center for Quality, Safety and Risk Assessment of Grass and Livestock Products, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.

Exorista civilis Rondani (Diptera:Tachinidae) is an excellent dominant parasitic enemy all over the world. But there has been a lack of research on the molecular regulation of diapause in E. civilis. Read More

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Analysis of current SARS-CoV-2 infection in a large population of blood donors evidenced that RNAemia is rare in plasma.

Transfusion 2021 Apr 16. Epub 2021 Apr 16.

Serviço de Pesquisa, Fundação Hemominas, Belo Horizonte, Minas Gerais, Brazil.

Background: Transmission of SARS-CoV-2 by asymptomatic individuals and by blood transfusion are important issues to understand in order to control the viral spread. In this work, we estimated the current SARS-CoV-2 infection rate in blood donors from Belo Horizonte, Brazil.

Study Design And Methods: Saliva and blood samples were collected from 4103 blood donors from June 15 to September 30, 2020. Read More

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Identification of miR-17, miR-21, miR-27a, miR-106b and miR-222 as endoplasmic reticulum stress-related potential biomarkers in circulation of patients with atherosclerosis.

Mol Biol Rep 2021 Apr 15. Epub 2021 Apr 15.

Department of Medical Biology, Faculty of Medicine, Yuksek Ihtisas University, Ankara, Turkey.

Atherosclerosis and related cardiovascular diseases are among the most common causes of death worldwide. Unfolded protein response, also known as Endoplasmic reticulum stress, has a critical role in many diseases including atherosclerosis. Small non-coding microRNAs (miRNA), which generally suppress gene expression, regulate UPR signalling and they may also be involved in the progression of atherosclerosis. Read More

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Identification and functional characterization of a new flavonoid synthase gene MdFLS1 from apple.

Planta 2021 Apr 15;253(5):105. Epub 2021 Apr 15.

School of Pharmacy, Liaocheng University, Liaocheng, Shandong, 250000, People's Republic of China.

Main Conclusion: The flavonoid synthase gene MdFLS1 from apple, which possibly plays an important role in anthocyanin synthesis, accumulates in the purple-red branches of Malus 'Pink spire'. Flavonoid metabolism serves an important function in plant growth and development. In this study, we selected 20 varieties of apple lines, 10 green and ten red branches, from the plant nursery of Qingdao Agriculture Academy. Read More

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Transcriptional Enhancer Factor Domain Family member 4 Exerts an Oncogenic Role in Hepatocellular Carcinoma by Hippo-Independent Regulation of Heat Shock Protein 70 Family Members.

Hepatol Commun 2021 Apr 20;5(4):661-674. Epub 2021 Jan 20.

Institute of Medical Genetics and Pathology University Hospital Basel Basel Switzerland.

Transcriptional enhancer factor domain family member 4 (TEAD4) is a downstream effector of the conserved Hippo signaling pathway, regulating the expression of genes involved in cell proliferation and differentiation. It is up-regulated in several cancer types and is associated with metastasis and poor prognosis. However, its role in hepatocellular carcinoma (HCC) remains largely unexplored. Read More

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Potential risk factors of avian influenza virus infection in asymptomatic commercial chicken flocks in selected areas of Bangladesh during 2019.

J Adv Vet Anim Res 2021 Mar 5;8(1):51-57. Epub 2021 Mar 5.

National Reference Laboratory for Avian Influenza, Animal Health Research Division, Bangladesh Livestock Research Institute, Dhaka, Bangladesh.

Objective: Avian influenza is a zoonotic disease with a pandemic potential that can infect avian and mammalian species, including humans. Studies aimed at investigating avian influenza virus (AIV) status in asymptomatic chickens and their shedding are uncommon in Bangladesh. Therefore, the current study aimed to examine the distribution of AIV subtypes in asymptomatic commercial chicken flocks and to identify the possible risk factors associated with this infection in two selected sub-districts of Bangladesh. Read More

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SYVN1-MTR4-MAT2A Signaling Axis Regulates Methionine Metabolism in Glioma Cells.

Front Cell Dev Biol 2021 30;9:633259. Epub 2021 Mar 30.

Department of Neurosurgery, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, China.

Methionine is one of the essential amino acids. How tumor cells adapt and adjust their signal transduction networks to avoid apoptosis in a methionine-restricted environment is worthy of further exploration. In this study, we investigated the molecular mechanism of glioma response to methionine restriction, providing a theoretical basis for new treatment strategies for glioma. Read More

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