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Current Strategies for Modulating Aβ Aggregation with Multifunctional Agents.

Acc Chem Res 2021 Apr 21. Epub 2021 Apr 21.

Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China.

ConspectusAlzheimer's disease (AD), as the primary cause of dementia, has seriously affected millions of people worldwide and brought a very heavy financial and social burden. With the growth of population and aging, the situation will worsen unless efficacious drugs are found to reverse, stop, or even slow down disease progression. More and more evidence has demonstrated that amyloid-β (Aβ) aggregation is an upstream causative factor in AD pathogenesis and then triggers a slew of pathological events. Read More

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Measurement of Nucleotide Hydrolysis Using Fluorescent Biosensors for Phosphate.

Methods Mol Biol 2021 ;2263:289-318

Structural Biology Science Technology Platform, The Francis Crick Institute, London, UK.

Assays for the detection of inorganic phosphate (P) are widely used to measure the activity of nucleotide hydrolyzing enzymes, such as ATPases and GTPases. The fluorescent biosensors for P, described here, are based on fluorescently labeled versions of E. coli phosphate-binding protein (PBP), which translates P binding into a large change in fluorescence intensity. Read More

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January 2021

SIRT2 and SIRT3 expression correlates with redox imbalance and advanced clinical stage in patients with multiple myeloma.

Clin Biochem 2021 Apr 13. Epub 2021 Apr 13.

Department of Clinical and Experimental Medicine, University of Messina, Messina Italy.

Objectives: Sirtuins comprise seven family elements (SIRT1-7) involved in various cell signalling pathways comprising cancer inhibition and tumorigenesis. The present study aims to evaluate SIRT2 and SIRT3 gene expression and potential redox reactions in patients with multiple myeloma (MM) at onset and its correlation with disease status, extent and presence of organ damage secondary to myeloma.

Design & Methods: Total RNA was extracted from 17 MM patients and 10 controls to assess gene expression using real-time PCR. Read More

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Multimodal Analysis of the Tissue Response to a Bone-Anchored Hearing Implant: Presentation of a Two-Year Case Report of a Patient With Recurrent Pain, Inflammation, and Infection, Including a Systematic Literature Review.

Front Cell Infect Microbiol 2021 30;11:640899. Epub 2021 Mar 30.

Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Osseointegration is a well-established concept used in applications including the percutaneous Bone-Anchored Hearing System (BAHS) and auricular rehabilitation. To date, few retrieved implants have been described. A systematic review including cases where percutaneous bone-anchored implants inserted in the temporal bone were retrieved and analyzed was performed. Read More

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Photocatalytic Surface Restructuring in Individual Silver Nanoparticles.

ACS Catal 2021 Mar 3;11(6):3478-3486. Epub 2021 Mar 3.

DIFFER-Dutch Institute for Fundamental Energy Research, De Zaale 20, 5612 AJ Eindhoven, The Netherlands.

Light absorption and scattering by metal nanoparticles can drive catalytic reactions at their surface via the generation of hot charge carriers, elevated temperatures, and focused electromagnetic fields. These photoinduced processes can substantially alter the shape, surface structure, and oxidation state of surface atoms of the nanoparticles and therefore significantly modify their catalytic properties. Information on such local structural and chemical change in plasmonic nanoparticles is however blurred in ensemble experiments, due to the typical large heterogeneity in sample size and shape distributions. Read More

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Colorectal cancer screening using a stool DNA-based SDC2 methylation test: a multicenter, prospective trial.

BMC Gastroenterol 2021 Apr 15;21(1):173. Epub 2021 Apr 15.

Department of Surgery, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, 892 Dongnam-ro, Seoul, Gangdong-gu, 05278, Korea.

Background: Prevention and early detection of colorectal cancer (CRC) is a global priority, with many countries conducting population-based CRC screening programs. Although colonoscopy is the most accurate diagnostic method for early CRC detection, adherence remains low because of its invasiveness and the need for extensive bowel preparation. Non-invasive fecal occult blood tests or fecal immunochemical tests are available; however, their sensitivity is relatively low. Read More

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Pre-coated interface proximity extension reaction assay enables trace protein detection with single-digit accuracy.

Biosens Bioelectron 2021 Apr 6;183:113211. Epub 2021 Apr 6.

State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China. Electronic address:

Advances in trace protein detection contribute to the early diagnosis of diseases and exploration of stem cell development. The pre-coated interface proximity extension reaction (PIPER) assay enables target protein detection at trace levels and was developed based on protein biomarker recognition using sets of three specific antibodies and the extension of antibody-bound nucleic acid chains in proximity, accompanied by amplification and reading of protein signals via real-time quantitative polymerase chain reaction (qPCR). Noise generated in binding reactions and enzymatic steps was decreased by transferring the liquid-liquid reactions onto a liquid-solid interface in glutaraldehyde-treated tubes pre-coated with antibodies. Read More

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Versatile and flexible microfluidic qPCR test for high-throughput SARS-CoV-2 and cellular response detection in nasopharyngeal swab samples.

PLoS One 2021 14;16(4):e0243333. Epub 2021 Apr 14.

Université Côte d'Azur, CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, FHU-OncoAge, Valbonne, France.

The emergence and quick spread of SARS-CoV-2 has pointed at a low capacity response for testing large populations in many countries, in line of material, technical and staff limitations. The traditional RT-qPCR diagnostic test remains the reference method and is by far the most widely used test. These assays are limited to a few probe sets, require large sample PCR reaction volumes, along with an expensive and time-consuming RNA extraction step. Read More

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The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis.

Sci Rep 2021 Apr 13;11(1):8088. Epub 2021 Apr 13.

Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Harvard Medical School, 16th Street Building (114-3503), Charlestown, MA, 02129, USA.

An excessive intestinal inflammatory response may have a role in the pathogenesis of necrotizing enterocolitis (NEC) in very preterm infants. Indole-3-lactic acid (ILA) of breastmilk tryptophan was identified as the anti-inflammatory metabolite involved in probiotic conditioned media from Bifidobacteria longum subsp infantis. This study aimed to explore the molecular endocytic pathways involved in the protective ILA effect against inflammation. Read More

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Bone marrow-derived mesenchymal stem cells attenuate silica-induced pulmonary fibrosis by inhibiting apoptosis and pyroptosis but not autophagy in rats.

Ecotoxicol Environ Saf 2021 Apr 10;216:112181. Epub 2021 Apr 10.

Department of Occupational Health and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address:

This study aimed to investigate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on silica-induced lung fibrosis in a rat model. Thirty SD rats were randomly divided into three groups: control group, silica group, and BMSC group (n = 10 rats per group). BMSCs were injected successively into rats on the 14th, 28th, and 42nd days after silica exposure. Read More

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Single-Molecule Tethered Particle Motion Studies on the DNA Recombinase Filament Assembly and Disassembly.

Methods Mol Biol 2021 ;2281:135-149

Department of Chemistry, National Taiwan University, Taipei, Taiwan.

Bacterial RecA and eukaryotic Rad51 are recombinases indispensable for DNA homologous recombination and repair of double-stranded DNA breaks. Understanding the functions and biophysical properties of the DNA recombinases benefits the research in human medicine such as cancer biology. Single-molecule techniques provide the mechanistic details of complex biological reactions. Read More

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January 2021

Circulating miR-449a predicts survival outcome for colorectal cancer following curative resection: An observational study.

Medicine (Baltimore) 2021 Apr;100(15):e25022

Department of VIP Medical Services, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.

Abstract: Previous studies showed that microRNA (miR)-449a may function as a tumor suppressor. However, the expression pattern and value of circulating miR-449a in colorectal cancer (CRC) remain unclear. Therefore, the purpose of this study was to measure circulating miR-449a level of CRC patients and evaluate its value for predicting prognosis. Read More

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Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7.

Pol J Vet Sci 2021 Mar;24(1):43-49

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.

In this study, we developed a SYBR Green I real-time PCR method for the rapid and sensitive detection of novel porcine parvovirus 7 (PPV7). Specific primers were designed based on the highly conserved region within the Capsid gene of PPV7. The established method was 1,000 times more sensitive than the conventional PCR method and had a detection limit of 35. Read More

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Social Network Analysis of the Effects of a Social Media-Based Weight Loss Intervention Targeting Adults of Low Socioeconomic Status: Single-Arm Intervention Trial.

J Med Internet Res 2021 Apr 9;23(4):e24690. Epub 2021 Apr 9.

Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH, United States.

Background: Obesity is a known risk factor for cardiovascular disease risk factors, including hypertension and type II diabetes. Although numerous weight loss interventions have demonstrated efficacy, there is considerably less evidence about the theoretical mechanisms through which they work. Delivering lifestyle behavior change interventions via social media provides unique opportunities for understanding mechanisms of intervention effects. Read More

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A mixing microfluidic chip for real-time NMR monitoring of macromolecular reaction.

J Biochem 2021 Apr 8. Epub 2021 Apr 8.

JEOL RESONANCE Inc., 3-1-2 Musashino, Akishima, Tokyo, 1968558, Japan.

NMR spectroscopy permits real-time monitoring of reactions that involve changes in the spectra of reactants. MICCS (MIcro Channeled Cell for Synthesis monitoring) is a microfluidic chip for such purposes, which is used to rapidly activate reactions by mixing the reactant solutions in the chip inserted into the typical NMR tube. Although it allows monitoring of chemical reactions of small compounds, its simple mixing system dependent on diffusion in the microchannel was not suitable for macromolecules such as proteins with low diffusion rates. Read More

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SARS-CoV-2 show no infectivity at later stages in a prolonged COVID-19 patient despite positivity in RNA testing.

J Med Virol 2021 Apr 8. Epub 2021 Apr 8.

Department of Pathology, University of Missouri, Columbia, Missouri, USA.

Inpatient COVID-19 cases present enormous costs to patients and health systems in the United States. Many hospitalized patients may continue testing COVID-19 positive even after resolution of symptoms. Thus, a pressing concern for clinicians is the safety of discharging these asymptomatic patients if they have any remaining infectivity. Read More

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Interleukin-6 via Toll-Like Receptor 3 Signaling Attenuates the Expression of Proinflammatory Chemokines in Human Podocytes.

Kidney Blood Press Res 2021 7;46(2):207-218. Epub 2021 Apr 7.

Department of Pediatrics, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.

Background: Although toll-like receptor 3 (TLR3) signaling is involved in the development of certain chronic kidney diseases, the specific molecular mechanisms underlying inflammatory reactions via activation of TLR3 signaling in human podocytes remain unclear. Interleukin (IL)-6 is a pleiotropic cytokine associated with innate and adaptive immune responses; however, little is known about the implication of IL-6 via the activation of regional TLR3 signaling in the inflammatory reactions in human podocytes.

Methods: We treated immortalized human podocytes with polyinosinic-polycytidylic acid (poly IC), an authentic viral double-stranded RNA, and assessed the expression of IL-6, monocyte chemoattractant protein-1 (MCP-1), and C-C motif chemokine ligand 5 (CCL5) using quantitative real-time reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. Read More

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Bipolar electrochemiluminescence imaging: a way to investigate the passivation of silicon surfaces.

Chemphyschem 2021 Apr 7. Epub 2021 Apr 7.

Commissariat à l'Energie Atomique Grenoble, CREAB, FRANCE.

This work depicts the original combination of electrochemiluminescence (ECL) and bipolar electrochemistry (BPE) to map in real-time the oxidation of silicon in microchannels. We fabricated model silicon-PDMS microfluidic chips, optionally containing a restriction, and monitored the evolution of the surface reactivity using ECL. BPE was used to remotely promote ECL at the silicon surface inside microfluidic channels. Read More

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In situ and operando electron microscopy in heterogeneous catalysis-insights into multi-scale chemical dynamics.

J Phys Condens Matter 2021 Apr 7;33(15):153001. Epub 2021 Apr 7.

Department of Interface Science, Fritz Haber Institute of the Max Planck Society, 14195 Berlin, Germany.

This review features state-of-the-art in situ and operando electron microscopy (EM) studies of heterogeneous catalysts in gas and liquid environments during reaction. Heterogeneous catalysts are important materials for the efficient production of chemicals/fuels on an industrial scale and for energy conversion applications. They also play a central role in various emerging technologies that are needed to ensure a sustainable future for our society. Read More

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Effect of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis.

Evid Based Complement Alternat Med 2021 20;2021:6686815. Epub 2021 Mar 20.

People's Hospital, Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.

Fyn-STAT5 is considered to be the frontier signaling pathway of IgE-mediated allergic reactions related to mast cell activation, but research on allergic rhinitis (AR) has been rarely reported. Xingbi gel nasal drops (XGND) are a compound preparation of traditional Chinese medicine, which has the exact therapeutic efficacy on AR. The current study aimed to observe the effects of XGND on Fyn-STAT5 pathway in AR guinea pig nasal mucosal fibroblasts and further illuminate the possible therapeutic mechanism of XGND on AR. Read More

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Newly developed SYBR Green-based quantitative real-time PCRs revealed coinfection evidence of and in existing in Bangkok Metropolitan, Thailand.

Food Waterborne Parasitol 2021 Jun 15;23:e00119. Epub 2021 Mar 15.

Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Thailand Mahidol University, Thailand.

is a well-known pathogen causing eosinophilic meningitis associated with angiostrongyliasis. Humans, as accidental hosts, are infected by consuming undercooked snails containing third-stage larvae. is closely related to and has been described as a potential human pathogen. Read More

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polymorphism in clopidogrel-treated Montenegrin patients.

Open Life Sci 2021 18;16(1):142-149. Epub 2021 Feb 18.

Department of Pharmacology and Toxicology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia.

Clopidogrel is an antiplatelet drug that displays significant interindividual variability in treatment response. Its bioavailability depends on the function of P-glycoprotein (P-gp), which is coded by a highly polymorphic gene. Thus, the aim of this study was to investigate the effect of genetic polymorphism on clopidogrel efficacy and safety and to determine the frequency distribution of its most common single nucleotide polymorphisms (SNPs) in 106 Montenegrin cardiology patients. Read More

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February 2021

Comparison of High-Resolution Computed Tomography and Real-Time Reverse Transcriptase Polymerase Chain Reaction in Diagnosis of COVID-19 Pneumonia in Intensive Care Unit Population.

Cureus 2021 Feb 28;13(2):e13602. Epub 2021 Feb 28.

Medicine, Jinnah Postgraduate Medical Centre, Karachi, PAK.

Introduction The efficacy of high-resolution computed tomography (HRCT) chest in common respiratory infections is well-established; however, its use in the diagnosis of COVID-19 pneumonia is less popular. The previous studies have failed to establish the efficacy of HRCT in the diagnosis of COVID-19 pneumonia. Objective The current study aimed to assess the efficacy of HRCT as compared to a polymerase chain reaction (PCR) in diagnosing COVID-19 pneumonia in patients in our setting. Read More

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February 2021

Two Zn(II)-based Coordination Polymers: Treatment Activity on Chronic Periodontitis by Inhibiting the Relative Expression of the Porphyria gingivalis Survival Gene.

J Oleo Sci 2021 ;70(4):541-548

Department of Periodontology, Qingdao Stomatological Hospital.

Two mixed-ligand complexes on the basis of L ligand [L = 3,6-bis(imidazol-1-yl)pyridazine] have been prepared under the solvothermal reaction conditions via the Zn(II) salts reacting with the ligands of L in the existence of two positional isomerous carboxylic acid ligands and their chemical formula respectively are [Zn(L)(1,2-BDC)(μ-OH)] (1, 1,2-HBDC = 1,2-benzenedicarboxylic acid ) and {[Zn(L)(1,3-HBDC) (1,3-BDC)(μ-OH)]·ClO·3HO} (2, 1,3-HBDC = 1,3-benzenedicarboxylic acid). The inhibitory influence of the two compounds against the inflammatory response in periodontium was evaluated by measuring the inflammatory cytokines releasing with ELISA detection kit. The results of ELISA assay indicated that compound 1 showed much stronger inhibitory influences than compound 2 against the inflammatory cytokines releasing. Read More

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January 2021

Real-Time Musculoskeletal Kinematics and Dynamics Analysis Using Marker- and IMU-Based Solutions in Rehabilitation.

Sensors (Basel) 2021 Mar 5;21(5). Epub 2021 Mar 5.

Department of Electrical and Computer Engineering, University of Patras, 26504 Patras, Greece.

This study aims to explore the possibility of estimating a multitude of kinematic and dynamic quantities using subject-specific musculoskeletal models in real-time. The framework was designed to operate with marker-based and inertial measurement units enabling extensions far beyond dedicated motion capture laboratories. We present the technical details for calculating the kinematics, generalized forces, muscle forces, joint reaction loads, and predicting ground reaction wrenches during walking. Read More

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Multiplex Real-Time PCR Assay for Six Major Carbapenemase Genes.

Pathogens 2021 Mar 1;10(3). Epub 2021 Mar 1.

Division of Infection Control and Prevention, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka 565-0871, Japan.

The global dissemination of carbapenemase-producing (CPE) is a major concern in public health. Due to the existence of the diversity of carbapenemases, development of an easily available, cost-effective multiplex detection assay for CPE is required worldwide. Using clinically available and reliable equipment, COBAS z480 (Roche Diagnostics K. Read More

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Crystallization Kinetics in BaTiO Synthesis from Hydrate Precursors via Microwave-Assisted Heat Treatment.

Nanomaterials (Basel) 2021 Mar 17;11(3). Epub 2021 Mar 17.

Department of Materials Science and Engineering, Inha University, Nam-gu, Incheon 22212, Korea.

The crystallization kinetics in BaTiO synthesis from hydrate precursors via microwave-assisted heating (MWH) were investigated. The structural and chemical features of powders synthesized via MWH and conventional heating (CH) were compared. The charged radicals generated under microwave irradiation were identified by chemical analysis and real-time charge flux measurements. Read More

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Quantification of the Tradeoff between Test Sensitivity and Test Frequency in a COVID-19 Epidemic-A Multi-Scale Modeling Approach.

Viruses 2021 03 11;13(3). Epub 2021 Mar 11.

Department of Mathematics, Virginia Tech, Blacksburg, VA 24060, USA.

Control strategies that employ real time polymerase chain reaction (RT-PCR) tests for the diagnosis and surveillance of COVID-19 epidemic are inefficient in fighting the epidemic due to high cost, delays in obtaining results, and the need of specialized personnel and equipment for laboratory processing. Cheaper and faster alternatives, such as antigen and paper-strip tests, have been proposed. They return results rapidly, but have lower sensitivity thresholds for detecting virus. Read More

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Real Time Observation of Lithium Insertion into Pre-Cycled Conversion-Type Materials.

Nanomaterials (Basel) 2021 Mar 14;11(3). Epub 2021 Mar 14.

Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, NY 11973, USA.

Conversion-type electrode materials for lithium-ion batteries experience significant structural changes during the first discharge-charge cycle, where a single particle is taken apart into a number of nanoparticles. This structural evolution may affect the following lithium insertion reactions; however, how lithiation occurs in pre-cycled electrode materials is elusive. In this work, in situ transmission electron microscopy was employed to see the lithium-induced structural and chemical evolutions in pre-cycled nickel oxide as a model system. Read More

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Atg16L1 as a Novel Biomarker and Autophagy Gene for Diabetic Retinopathy.

J Diabetes Res 2021 18;2021:5398645. Epub 2021 Mar 18.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.

Objective: Accumulating evidence suggests the critical role of autophagy in the pathogenesis of diabetic retinopathy (DR). In the current study, we aim to identify autophagy genes involved in DR via microarray analyses.

Methods: Gene microarrays were performed to identify differentially expressed lncRNAs/mRNAs between normal and DR retinas. Read More

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