1,717,347 results match your criteria rats


HEPATOPROTECTIVE EFFICIENCY OF G10 SUBSTANCE FROM ZHUZGUN PLANT IN EXPERIMENTAL TOXIC HEPATITIS.

Georgian Med News 2021 Mar(312):138-145

NJCC"Astana Medical University", Department of General Pharmacology, Nur-Sultan, Kazakhstan.

The article presents the results of biochemical studies of blood and morphological characteristics of liver changes in laboratory animals (rats) under experimental conditions of paracetamol hepatitis and intragastric administration of a new substance G10 from the «Zhuzgun» plant in various doses. The obtained data open up prospects for further studies of the pharmacological properties of the substance G10, the possibility of including it as a phytotherapeutic agent in the complex of preventive and therapeutic measures for acute toxic hepatitis. The study of the hepatoprotective properties was conducted in the "Educational and Research Pharmacological Laboratory" of the Department of General Pharmacology of the Astana Medical University. Read More

View Article and Full-Text PDF

ANTIHYPERTENSIVE AND CARDIOPROTECTIVE EFFECTS OF EPOXYEICOSATRIENOIC ACID ANALOGS AND SOLUBLE EPOXIDE HYDROLASE INHIBITORS (REVIEW).

Georgian Med News 2021 Mar(312):125-132

Tbilisi State Medical University, Departments of Pharmaceutical Technology and Medical Pharmacology, Georgia.

In the present article is considered antihypertensive and cardioprotective action of epoxieicosatrienoic acids (EETs) analogues and soluble epoxide hydrolase (sEH) inhibitors. Being epoxygenase products of arachidonic acid metabolism EETs provide a wide spectrum of biological activity in different organs implicated in the regulation of arterial pressure, including vascular endothelium (VE), heart and kidney. EETs are acting as autocrine or paracrine agents producing vasodilation, reduction in salt and fluid retention. Read More

View Article and Full-Text PDF

NORMALIZING EFFECT OF ELECTROMAGNETIC STIMULATION ON BLOOD QUANTITATIVE INDICES IN DEPRESSED RATS ON THE BACKGROUND OF OXYTOCIN.

Georgian Med News 2021 Mar(312):100-104

3European University, Tbilisi, Georgia.

Depression is linked to systemic inflammation. There is an association between blood cell count and/or hematological inflammatory markers with depression symptoms. Oxytocin reduces the activity of brain regions that produce anxiety and might involve in the rehabilitation of depressive-like behavior. Read More

View Article and Full-Text PDF

Evaluation of the chemopreventive effects of Hypericum perforatum L on DMBA-applied rat oral mucosa.

Arch Oral Biol 2021 Apr 30;127:105139. Epub 2021 Apr 30.

Ankara University, Faculty of Medicine, Department of Medical Biochemistry, Ankara, Turkey.

Objective: Hypericum perforatum L also known as St. John's wort is known to have many beneficial properties for the organism including its antioxidant and anticancer activities. It is also known to have shown antiproliferative and cytotoxic effects against various cancer cell lines. Read More

View Article and Full-Text PDF

Neuroprotective effect of 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline mediated via regulation of antioxidant system and inhibition of inflammation and apoptosis in a rat model of cerebral ischemia/reperfusion.

Biochimie 2021 May 5. Epub 2021 May 5.

Department of Pathological Anatomy, Voronezh State Medical University named after N.N. Burdenko, Russia.

The aim of the study was the assessment of the neuroprotective potential of 6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline (DHQ) and its effect on inflammation, apoptosis, and transcriptional regulation of the antioxidant system in cerebral ischemia/reperfusion (CIR) in rats. The CIR rat model was constructed using the bilateral common carotid artery occlusion followed by reoxygenation. DHQ was administered at a dose of 50 mg/kg for three days. Read More

View Article and Full-Text PDF

Metabolomics Based Comprehensive Investigation of Gardeniae Fructus Induced Hepatotoxicity.

Food Chem Toxicol 2021 May 5:112250. Epub 2021 May 5.

School of Pharmacy, Second Military Medical University, Shanghai, 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China. Electronic address:

Gardeniae Fructus (Zhizi in Chinese, ZZ in brief), a commonly used herbal medicine, has aroused wide concern for hepatotoxicity, but the mechanism remains to be investigated. This study was aimed at investigating the mechanism of ZZ-induced liver injury in vivo and in vitro based on metabolomics and evaluating the hepatotoxicity prediction ability of the in vitro model. SD rats were administered with extracted ZZ and HepG2 cells were treated with genipin, the major hepatotoxic metabolite of ZZ. Read More

View Article and Full-Text PDF

Genotoxicity of Asiasari Radix et Rhizoma (Aristolochiaceae) ethanolic extract in vitro and in vivo.

J Ethnopharmacol 2021 May 5:114122. Epub 2021 May 5.

Korea Institute of Oriental Medicine, 1672 Expo-ro, Yuseong-gu, Daejeon 34054, Republic of Korea. Electronic address:

Ethnopharmacological Relevance: Traditional herbal medicines have diverse efficacy and are increasingly used worldwide. However, some of these herbal medicines have toxicities or side effects, but the scientific understanding of traditional herbal medicine toxicity has not yet been established. Asiasari Radix et Rhizoma (ARE) is known as a herbal medicine used to relieve pain, and recent studies have shown that ARE has anticancer and antimelanogenesis efficacy. Read More

View Article and Full-Text PDF

Resurgence of Alcohol Seeking Following Abstinence Induced by Punishment in Male and Female Rats.

Behav Brain Res 2021 May 5:113345. Epub 2021 May 5.

Utah State University, United States. Electronic address:

To better approximate the human condition, animal models of relapse to drug and alcohol seeking have increasingly employed negative consequences to generate abstinence. Here we report the first demonstration of relapse to punishment-suppressed alcohol seeking induced by loss of non-drug reward (i.e. Read More

View Article and Full-Text PDF

Transplantation of Rat Dental Pulp Stem Cells Facilities Post-Lesion Recovery in the Somatosensory Whisker Cortex of Male Wistar Rats.

Brain Res Bull 2021 May 5. Epub 2021 May 5.

Neuroscience Research Center, Institute of Neuropharmachology, Kerman University of Medical Sciences, Kerman, Iran; Cognitive Neuroscience Research Center, Institute of Neuropharmachology, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

Damage to somatosensory "barrel" cortex reduces the rats' behavioral sensitivity in discrimination of tactile stimuli. Here, we examined how transplantation of stem cells into the lesioned barrel cortex can help in recovery of sensory capacities. We induced mechanical lesions in the right barrel cortex area of male rats. Read More

View Article and Full-Text PDF

Voluntary wheel-running exercise attenuates brain aging of rats through activating miR-130a-mediated autophagy.

Brain Res Bull 2021 May 5. Epub 2021 May 5.

Hubei Key Laboratory of Sport Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan, 430079, China. Electronic address:

Autophagy is a highly regulated intracellular process for the degradation of protein aggregates and damaged organelles. Recently, autophagy has been implicated in Alzheimer's disease (AD) and aging. Autophagy process is regulated by the recruitment and assembly of several autophagy-related genes (Atgs) such as, Atg7 and LC3, as the highly conserved and important markers involved in the regulation of autophagy. Read More

View Article and Full-Text PDF

Enhanced neuroprotective and antidepressant activity of curcumin-loaded nanostructured lipid carriers in lipopolysaccharide-induced depression and anxiety rat model.

Int J Pharm 2021 May 5:120670. Epub 2021 May 5.

Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan. Electronic address:

The present study aims to develop curcumin-loaded nanostructured lipid carriers (CUR-NLCs) and investigate their neuroprotective effects in lipopolysaccharide (LPS)-induced depression and anxiety model. Nanotemplate engineering technique was used to prepare CUR-NLCs with Compritol 888 ATO and oleic acid as solid and liquid lipid, respectively. Poloxamer 188, Tween 80 and Span 80 were used as stabilizing agents for solid-liquid lipid core. Read More

View Article and Full-Text PDF

Optogenetic stimulation of entorhinal cortex reveals the implication of insulin signaling in adult rat's hippocampal neurogenesis.

Prog Neuropsychopharmacol Biol Psychiatry 2021 May 5:110344. Epub 2021 May 5.

Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Adult neurogenesis in the hippocampal dentate gyrus plays a critical role in learning and memory. Projections originating from entorhinal cortex, known as the perforant pathway, provide the main input to the dentate gyrus and promote neurogenesis. However, neuromodulators and molecular changes mediating neurogenic effects of this pathway are not yet fully understood. Read More

View Article and Full-Text PDF

Trichinella spiralis excretory-secretory products downregulate MMP-9 in Dark Agouti rats affected by experimental autoimmune encephalomyelitis.

Exp Parasitol 2021 May 5:108112. Epub 2021 May 5.

Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31b, 11080, Belgrade, Serbia.

Matrix metalloproteinases (MMPs), are implicated in the pathogenesis of multiple sclerosis (MS) and in its animal model, experimental autoimmune encephalomyelitis (EAE). Our aim was to investigate whether amelioration of EAE in Dark Agouti (DA) rats, induced by Trichinella spiralis muscle larvae excretory-secretory products (ES L1), could be related to the level and activity of gelatinases, MMP-9 and MMP-2. Serum levels of MMP-9, MMP-2, NGAL/MMP-9, TIMP-1, and cytokines, evaluated by gel-zymography or ELISA, as well as gelatinases and TIMP-1 expression in the spinal cord (SC), were determined in: i) EAE induced, ii) ES L1-treated EAE induced animals. Read More

View Article and Full-Text PDF

Ergothioneine mitigates cisplatin-evoked nephrotoxicity via targeting Nrf2, NF-κB, and apoptotic signaling and inhibiting γ-glutamyl transpeptidase.

Life Sci 2021 May 5:119572. Epub 2021 May 5.

Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Cairo 11751, Egypt.

Aim: Cisplatin is a potent chemotherapeutic agent whose therapeutic application is hindered by the associated nephrotoxicity. Cisplatin-evoked nephrotoxicity has been largely attributed to the induction of oxidative stress and inflammatory responses. The current study aimed at investigating the ability of ergothioneine to mitigate cisplatin-evoked nephrotoxicity and to elucidate the underlining molecular mechanisms. Read More

View Article and Full-Text PDF

Energy management and mitochondrial dynamics in cerebral cortex during endotoxemia.

Arch Biochem Biophys 2021 May 5:108900. Epub 2021 May 5.

Universidad de Buenos Aires, CONICET, Instituto de Bioquímica y Medicina Molecular (IBIMOL), Facultad de Farmacia y Bioquímica, Junín 946, C1113AAD, CABA, Argentina; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Química Analítica y Fisicoquímica, Cátedra de Fisicoquímica, Junín 946, C1113AAD, CABA, Argentina. Electronic address:

Mitochondria play an essential role in inflammatory processes such as sepsis or endotoxemia, contributing to organ-cellular redox metabolism, emerging as the energy hub of the cell, and as an important center of action of second messengers. In this work, we aimed to elucidate the energy state, redox balance, and mitochondrial remodeling status in cerebral cortex in an experimental model of endotoxemia. Female Sprague-Dawley rats were subjected to a single dose of LPS (ip 8 mg kg body weight) for 6 h. Read More

View Article and Full-Text PDF

Uterine contractile activity and fetal outcome in rats treated with vitamin C during late gestational variable stress exposure.

J Complement Integr Med 2021 May 10. Epub 2021 May 10.

Department of Physiology, Lagos State University College of Medicine, Ikeja, Lagos State, Nigeria.

Objectives: Stress responses vary throughout pregnancy and impact of late gestational variable stress (LGVS) with vitamin C supplementation on uterine contractility is barely explored. This study investigates fetal weight outcome and uterine contractile responses to pharmacological agents during LGVS exposure.

Methods: Twenty four nulliparous pregnant rats were divided into four groups of six. Read More

View Article and Full-Text PDF

Effects of co-administration of pharmacopoeia formulations and with CAT-I antitubercular drugs in rats.

J Complement Integr Med 2021 May 10. Epub 2021 May 10.

ICMR-National Institute for Research in Tuberculosis (NIRT), Chennai, Tamil Nadu, India.

Objectives: Tuberculosis continues to be a major public health problem globally, despite incredible advancements in healthcare system. In system of medicine, (QTS) and (AHB) have been traditionally used for tuberculosis like conditions. The study was aimed to investigate the effects of co-administration of QTS and AHB with category I first line antitubercular drugs (CAT-I) on the indices of liver and kidney function in rats. Read More

View Article and Full-Text PDF

Intrahippocampal co-administration of nicotine and O-acetyl-L-carnitine prevents the H-89-induced spatial learning deficits in Morris water maze.

J Complement Integr Med 2021 May 10. Epub 2021 May 10.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.

Objectives: H-89 (a protein kinase AII [PKA II] inhibitor) impairs the spatial memory in the Morris water maze task in rats. In the present study, we aimed to study the protective effects of nicotine and O-acetyl-L-carnitine against H-89-induced spatial memory deficits.

Methods: Spatial memory impairment was induced by the bilateral intrahippocampal administration of 10 µM H-89 (dissolved in dimethyl sulfoxide, DMSO) to rats. Read More

View Article and Full-Text PDF

Hippocampal subfield-specific Homer1a expression is triggered by learning-facilitated long-term potentiation and long-term depression at medial perforant path synapses.

Hippocampus 2021 May 8. Epub 2021 May 8.

Medical Faculty, Department of Neurophysiology, Ruhr University Bochum, Bochum, Germany.

Learning about general aspects, or content details, of space results in differentiated neuronal information encoding within the proximodistal axis of the hippocampus. These processes are tightly linked to long-term potentiation (LTP) and long-term depression (LTD). Here, we explored the precise sites of encoding of synaptic plasticity in the hippocampus that are mediated by information throughput from the perforant path. Read More

View Article and Full-Text PDF

Conscious rat PET imaging with soft immobilization for quantitation of brain functions: comprehensive assessment of anesthesia effects on cerebral blood flow and metabolism.

EJNMMI Res 2021 May 8;11(1):46. Epub 2021 May 8.

Preeminent Medical Photonics Education and Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.

Background: Animal brain functions evaluated by in vivo imaging under anesthesia can be affected by anesthetic agents, resulting in incorrect assessment of physiological brain function. We therefore performed dynamic positron emission tomography (PET) imaging of conscious rats using recently reported soft immobilization to validate the efficacy of the immobilization for brain function assessments. We also determined the effects of six anesthetic agents-a mixed anesthetic agent (MMB), ketamine + xylazine (KX), chloral hydrate (Chloral), pentobarbital (PTB), propofol (PF), and isoflurane (IFL)-on brain function by comparison with conscious rats. Read More

View Article and Full-Text PDF

Preparation of loratadine nanocrystal tablets to improve the solubility and dissolution for enhanced oral bioavailability.

J Pharm Pharmacol 2021 May 8. Epub 2021 May 8.

State Key Laboratory of Natural Medicines, Department of Pharmaceutics, China Pharmaceutical University, Nanjing, China.

Objectives: Loratadine is a selective H1 receptor inhibitor that has been widely used in the clinical treatment of allergic diseases. Here we aimed to develop a novel solid loratadine nanocrystal to increase the low and pH-dependent water solubility for bioavailability enhancement.

Methods: Loratadine solid nanocrystal was developed through high-speed shear-high pressure homogenization followed by freeze-drying, which was further prepared into tablets through direct compression. Read More

View Article and Full-Text PDF

[Calpain activation promotes dialysis-associated peritoneal fibrosis in rats].

Authors:
F Li X Hong J Jiang

Nan Fang Yi Ke Da Xue Xue Bao 2021 Apr;41(4):593-599

Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To explore the role of calpain activation in the progression of peritoneal fibrosis.

Objective: Twenty-four male Sprague-Dawley rats were randomized equally into control group, MDL28170 (a calpain inhibitor)+normal saline group, peritoneal dialysis (PD) model group and PD + MDL28170 group. In the latter two groups, the rats received daily intraperitoneal injections of 100 mL/kg of 4. Read More

View Article and Full-Text PDF

[CRISPR/Cas9-mediated TEAD1 knockout induces phenotypic modulation of corpus cavernosum smooth muscle cells in diabetic rats with erectile dysfunction].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Apr;41(4):567-573

Department of Urology, Second Guangdong Provincial People's Hospital, Guangzhou 510317, China.

Objective: To construct a corpus cavemosum smooth muscle cell (CCSMCs) line with TEAD1 knockout from diabetic rats with erectile dysfunction (ED) using CRISPR/Cas9 technology and explore the role of TEAD1 in phenotypic modulation of CCSMCs in diabetic rats with ED.

Objective: Models of diabetic ED were established in male Sprague-Dawley rats by intraperitoneal injection of streptozotocin. CCSMCs from the rat models were primarily cultured and identified with immunofluorescence assay. Read More

View Article and Full-Text PDF

[Effect of protein C activator from venom on early adaptive immune response of septic rats].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Apr;41(4):514-520

School of Pharmacy, Wannan Medical College, Wuhu 241002, China.

Objective: To investigate the effect of protein C activator (PCA) from venom (AAV) in modulating early adaptive immune response of septic rats.

Objective: Rat models of sepsis were established by intraperitoneal injection of lipopolysaccharide (LPS; 10 mg/kg) in 36 SD rats, which were divided into 6 groups (=6) for sample collection at 4, 6, 8, 12, 16 and 24 h after LPS injection, with 6 rats injected with saline as the control group. Another 36 rats were divided into two groups, and 30 min after LPS injection, the rats were treated with SEW2871 (a sphingosine-1-phosphate receptor 1 agonist; 0. Read More

View Article and Full-Text PDF

Correction to: The Reproductive Injury and Oxidative Testicular Toxicity Induced by Chlorpyrifos Can Be Restored by Zinc in Male Rats.

Biol Trace Elem Res 2021 May 8. Epub 2021 May 8.

Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

View Article and Full-Text PDF

Alteration of twinfilin1 expression underlies opioid withdrawal-induced remodeling of actin cytoskeleton at synapses and formation of aversive memory.

Mol Psychiatry 2021 May 7. Epub 2021 May 7.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Exposure to drugs of abuse induces alterations of dendritic spine morphology and density that has been proposed to be a cellular basis of long-lasting addictive memory and heavily depend on remodeling of its underlying actin cytoskeleton by the actin cytoskeleton regulators. However, the actin cytoskeleton regulators involved and the specific mechanisms whereby drugs of abuse alter their expression or function are largely unknown. Twinfilin (Twf1) is a highly conserved actin-depolymerizing factor that regulates actin dynamics in organisms from yeast to mammals. Read More

View Article and Full-Text PDF

Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease.

Sci Rep 2021 May 7;11(1):9788. Epub 2021 May 7.

Department of Otolaryngology, Head and Neck Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.

Chronic kidney disease (CKD) leads to musculoskeletal impairments that are impacted by muscle metabolism. We tested the hypothesis that 10-weeks of voluntary wheel running can improve skeletal muscle mitochondria activity and function in a rat model of CKD. Groups included (n = 12-14/group): (1) normal littermates (NL); (2) CKD, and; (3) CKD-10 weeks of voluntary wheel running (CKD-W). Read More

View Article and Full-Text PDF

Author Correction: Direct measurement of vagal tone in rats does not show correlation to HRV.

Sci Rep 2021 May 7;11(1):10233. Epub 2021 May 7.

Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA.

View Article and Full-Text PDF

Repeated infusion of mesenchymal stem cells maintain the condition to inhibit deteriorated motor function, leading to an extended lifespan in the SOD1G93A rat model of amyotrophic lateral sclerosis.

Mol Brain 2021 May 7;14(1):76. Epub 2021 May 7.

Department of Neural Regenerative Medicine, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, 060-8556, Japan.

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative fatal disorder in which motor neurons within the brain and spinal cord degenerate. A single infusion of mesenchymal stem cells (MSCs) delays disease progression by protecting motor neurons and restoring the blood-spinal cord barrier in the SOD1G93A transgenic ALS rat model. However, the therapeutic effect of a single infusion of MSCs is transient and does not block disease progression. Read More

View Article and Full-Text PDF

Tributyltin perturbs femoral cortical architecture and polar moment of inertia in rat.

BMC Musculoskelet Disord 2021 May 7;22(1):427. Epub 2021 May 7.

Department of Environmental Health, School of Public Health, Cheeloo College of Medicine, Shandong University, 44 Wenhua Xi Lu, Jinan, 250012, Shandong, China.

Background: Tributyltin, a well-known endocrine disruptor, is widely used in agriculture and industry. Previous studies have shown that tributyltin could cause deleterious effects on bone health by impairing the adipo-osteogenic balance in bone marrow.

Methods: To investigate further the effects of tributyltin on bone, weaned male SD rats were treated with tributyltin (0. Read More

View Article and Full-Text PDF