462 results match your criteria rapidly a549

Repurposing the Ebola and Marburg Virus Inhibitors Tilorone, Quinacrine, and Pyronaridine: Activity against SARS-CoV-2 and Potential Mechanisms.

ACS Omega 2021 Mar 10;6(11):7454-7468. Epub 2021 Mar 10.

Collaborations Pharmaceuticals, Inc., 840 Main Campus Drive, Lab 3510, Raleigh, North Carolina 27606, United States.

Severe acute respiratory coronavirus 2 (SARS-CoV-2) is a newly identified virus that has resulted in over 2.5 million deaths globally and over 116 million cases globally in March, 2021. Small-molecule inhibitors that reverse disease severity have proven difficult to discover. Read More

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Chitosan nano-vehicles as biocompatible delivering tools for a new Ag(I)curcuminoid-Gboxin analog complex in cancer and inflammation therapy.

Int J Biol Macromol 2020 Dec 24;165(Pt B):2750-2764. Epub 2020 Oct 24.

Faculty of Science, Zoology Department, Suez University, 43533 Suez, Egypt. Electronic address:

A novel anticancer and anti-inflammatory agent based on hybrid curcuminoid-Gboxin analog (FLLL49-GbA) and its macromolecular silver(I) complex (Ag(I)FLLL49-GbA) have successfully synthesized. In addition, chitosan nanoparticles (CNPs) were used to encapsulate this macromolecular complex, targeting enhancing its therapeutic effect and minimizing its side impacts. The encapsulated Ag(I) complex was significantly triggered apoptosis (P < 0. Read More

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December 2020

A CD4+CD161+ T-Cell Subset Present in Unexposed Humans, Not Tb Patients, Are Fast Acting Cells That Inhibit the Growth of Intracellular Mycobacteria Involving CD161 Pathway, Perforin, and IFN-γ/Autophagy.

Front Immunol 2021 26;12:599641. Epub 2021 Feb 26.

Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, IL, United States.

It remains undefined whether a subset of CD4+ T cells can function as fast-acting cells to control (Mtb) infection. Here we show that the primary CD4+CD161+ T-cell subset, not CD4+CD161-, in unexposed healthy humans fast acted as unconventional T cells capable of inhibiting intracellular Mtb and BCG growth upon exposure to infected autologous and allogeneic macrophages or lung epithelial A549 cells. Such inhibition coincided with the ability of primary CD4+CD161+ T cells to rapidly express/secrete anti-TB cytokines including IFN-γ, TNF-α, IL-17, and perforin upon exposure to Mtb. Read More

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February 2021

Acoustic Droplet Vitrification Method for High-Efficiency Preservation of Rare Cells.

ACS Appl Mater Interfaces 2021 Mar 11;13(11):12950-12959. Epub 2021 Mar 11.

Key Laboratory of Artificial Micro/Nano-Structures, Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China.

Cryopreservation is a key step for current translational medicine including reproductive medicine, regenerative medicine, and cell therapy. However, it is challenging to preserve rare cells for practical applications due to the difficulty in handling low numbers of cells as well as the lack of highly efficient and biocompatible preservation protocols. Here, we developed an acoustic droplet vitrification method for high-efficiency handling and preservation of rare cells. Read More

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A Carrier-Free Folate Receptor-Targeted Ursolic Acid/Methotrexate Nanodelivery System for Synergetic Anticancer Therapy.

Int J Nanomedicine 2021 3;16:1775-1787. Epub 2021 Mar 3.

Department of Pharmaceutics, University of Washington, Seattle, WA, 98195, USA.

Purpose: To avoid undefined metabolic mechanisms and to eliminate potential side effects of traditional nanocarriers, new green carriers are urgently needed in cancer treatment. Carrier-free nanoparticles (NPs) based on ursolic acid (UA) have attracted significant attention, but the UA NPs targeting the folate receptor have never been explored. We designed a novel self-assembled UA-Methotrexate (MTX) NPs targeting the folate-receptor and its synergetic anticancer activity was studied in vitro and in vivo. Read More

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MicroRNA-147b alleviates inflammation and apoptosis in acute lung injury via inhibition of p38 MAPK signaling pathway.

Eur Rev Med Pharmacol Sci 2021 Feb;25(4):1974-1981

Department of Emergency Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Objective: The pathogenesis of acute lung injury (ALI) is complicated, the condition is developing rapidly, and the mortality rate is high. It is a common acute and critical illness in clinic. Here, we aimed to demonstrate the function and molecular mechanism of microRNA-147b (miR-147b) in ALI. Read More

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February 2021

Design, synthesis, and evaluation of a novel prodrug, a S-trityl--cysteine derivative targeting kinesin spindle protein.

Eur J Med Chem 2021 Apr 16;215:113288. Epub 2021 Feb 16.

Center for Drug Discovery, Graduate Division of Pharmaceutical Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka, 422-8526, Japan. Electronic address:

Kinesin spindle protein (KSP) is expressed only in cells undergoing cell division, and hence represents an attractive target for the treatment of cancer. Several KSP inhibitors have been developed and undergone clinical trial, but their clinical use is limited by their toxicity to rapidly proliferating non-cancerous cells. To create new KSP inhibitors that are highly selective for cancer cells, we optimized the amino acid moiety of S-trityl--cysteine (STLC) derivative 1 using in silico modeling. Read More

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Causal network models of SARS-CoV-2 expression and aging to identify candidates for drug repurposing.

Nat Commun 2021 02 15;12(1):1024. Epub 2021 Feb 15.

Massachusetts Institute of Technology, Cambridge, MA, USA.

Given the severity of the SARS-CoV-2 pandemic, a major challenge is to rapidly repurpose existing approved drugs for clinical interventions. While a number of data-driven and experimental approaches have been suggested in the context of drug repurposing, a platform that systematically integrates available transcriptomic, proteomic and structural data is missing. More importantly, given that SARS-CoV-2 pathogenicity is highly age-dependent, it is critical to integrate aging signatures into drug discovery platforms. Read More

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February 2021

The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types.

Elife 2021 02 11;10. Epub 2021 Feb 11.

New York Genome Center, New York, United States.

A novel variant of the SARS-CoV-2 virus carrying a point mutation in the Spike protein (D614G) has recently emerged and rapidly surpassed others in prevalence. This mutation is in linkage disequilibrium with an ORF1b protein variant (P314L), making it difficult to discern the functional significance of the Spike D614G mutation from population genetics alone. Here, we perform site-directed mutagenesis on wild-type human-codon-optimized Spike to introduce the D614G variant. Read More

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February 2021

Polyhexamethylene Guanidine Phosphate Induces Apoptosis through Endoplasmic Reticulum Stress in Lung Epithelial Cells.

Int J Mol Sci 2021 Jan 26;22(3). Epub 2021 Jan 26.

College of Pharmacy, Daegu Catholic University, Gyeongsan 38430, Korea.

Airway epithelial cell death contributes to the pathogenesis of lung fibrosis. Polyhexamethylene guanidine phosphate (PHMG-p), commonly used as a disinfectant, has been shown to be strongly associated with lung fibrosis in epidemiological and toxicological studies. However, the molecular mechanism underlying PHMG-p-induced epithelial cell death is currently unclear. Read More

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January 2021

Design, synthesis and biological evaluation of water soluble and non-aggregated silicon phthalocyanines, naphthalocyanines against A549, SNU-398, SK-MEL128, DU-145, BT-20 and HFC cell lines as potential anticancer agents.

Bioorg Chem 2021 Feb 9;107:104637. Epub 2021 Jan 9.

Karadeniz Technical University, Department of Chemistry, Trabzon, Turkey. Electronic address:

Cancer has become an important public problem in worldwide since cancer incidence and mortality are growing rapidly. In this study, water soluble and non-aggregated silicon (IV) phthalocyanines and naphthalocyanines containing (3,5-bis{3-[3-(diethylamino)phenoxy]propoxy}phenyl)methoxy groups have been synthesized and characterized to investigate their anticancer potential. Their DNA binding/nuclease, topoisomerases inhibition were investigated using UV-Vis absorption, thermal denaturation and agarose gel electrophoresis. Read More

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February 2021

ERK Activation-Mediated Autophagy Induction Resists Licochalcone A-Induced Anticancer Activities in Lung Cancer Cells .

Onco Targets Ther 2020 8;13:13437-13450. Epub 2021 Jan 8.

Department of Cardiology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan, People's Republic of China.

Introduction: The incidence and mortality rates of lung cancer rank top in the different types of cancers in China. Licochalcone A (LA) is a flavonoid extracted from the roots of licorice with antitumor effects in various cancers and . However, the role of LA in non-small cell lung cancer (NSCLC) remains largely unclear. Read More

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January 2021

Mapping the SARS-CoV-2-Host Protein-Protein Interactome by Affinity Purification Mass Spectrometry and Proximity-Dependent Biotin Labeling: A Rational and Straightforward Route to Discover Host-Directed Anti-SARS-CoV-2 Therapeutics.

Int J Mol Sci 2021 Jan 7;22(2). Epub 2021 Jan 7.

Department of Medical and Surgical Sciences, University "Magna Græcia", 88100 Catanzaro, Italy.

Protein-protein interactions (PPIs) are the vital engine of cellular machinery. After virus entry in host cells the global organization of the viral life cycle is strongly regulated by the formation of virus-host protein interactions. With the advent of high-throughput -omics platforms, the mirage to obtain a "high resolution" view of virus-host interactions has come true. Read More

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January 2021

Size-Dependent Bioactivity of Silver Nanoparticles: Antibacterial Properties, Influence on Copper Status in Mice, and Whole-Body Turnover.

Nanotechnol Sci Appl 2020 31;13:137-157. Epub 2020 Dec 31.

International Research Center of Functional Materials and Devices of Optoelectronics, ITMO University, St. Petersburg, Russia.

Purpose: The ability of silver nanoparticles (AgNPs) of different sizes to influence copper metabolism in mice is assessed.

Materials And Methods: AgNPs with diameters of 10, 20, and 75 nm were fabricated through a chemical reduction of silver nitrate and characterized by UV/Vis spectrometry, transmission and scanning electronic microscopy, and laser diffractometry. To test their bioactivity, cells, cultured A549 cells, and C57Bl/6 mice were used. Read More

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December 2020

Chemical Adducts of Reactive Flavor Aldehydes Formed in E-Cigarette Liquids Are Cytotoxic and Inhibit Mitochondrial Function in Respiratory Epithelial Cells.

Nicotine Tob Res 2020 12;22(Suppl 1):S25-S34

Department of Anesthesiology, Duke University School of Medicine, Durham, NC.

Introduction: Flavor aldehydes in e-cigarettes, including vanillin, ethyl vanillin (vanilla), and benzaldehyde (berry/fruit), rapidly undergo chemical reactions with the e-liquid solvents, propylene glycol, and vegetable glycerol (PG/VG), to form chemical adducts named flavor aldehyde PG/VG acetals that can efficiently transfer to e-cigarette aerosol. The objective of this study was to compare the cytotoxic and metabolic toxic effects of acetals and their parent aldehydes in respiratory epithelial cells.

Aims And Methods: Cell metabolic assays were carried out in bronchial (BEAS-2B) and alveolar (A549) epithelial cells assessing the effects of benzaldehyde, vanillin, ethyl vanillin, and their corresponding PG acetals on key bioenergetic parameters of mitochondrial function. Read More

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December 2020

A dual-mode strategy for sensing and bio-imaging of endogenous alkaline phosphatase based on the combination of photoinduced electron transfer and hyperchromic effect.

Anal Chim Acta 2021 Jan 30;1142:65-72. Epub 2020 Sep 30.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel Farming and Processing, Fuzhou University, Fuzhou, Fujian, 350108, PR China. Electronic address:

Benefit from the additional correction of the output signal in dual-mode detection, traditional dual signal readout strategies are performed by constructing the ratiometric fluorescent probe through excitation energy transfer (EET) or fluorescence resonance energy transfer (FRET). To avoid the complicated modification process and obtain the results rapidly, a simple dual-mode sensing strategy based on the electronic effects of p-nitrophenol (PNP) is described to monitor the activities of alkaline phosphatase (ALP). In the sensing platform, p-nitrophenylphosphate was used as a substrate to produce the PNP using ALP as the catalyst. Read More

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January 2021

JUUL e-liquid exposure elicits cytoplasmic Ca responses and leads to cytotoxicity in cultured airway epithelial cells.

Toxicol Lett 2021 Feb 27;337:46-56. Epub 2020 Nov 27.

Biomanufacturing Research Institute and Technology Enterprise (BRITE), Durham, NC, United States; Department of Pharmaceutical Sciences, North Carolina Central University, Durham, NC, United States. Electronic address:

Rationale: The popularity of new and emerging tobacco products such as E-cigarettes (E-cigs) is rapidly expanding worldwide. However, uncertainties surrounding the potential health consequences due to the use of such products exist and warrant further study.

Methods: Cultured A549 and Calu-3 airway epithelia were exposed to three out of the eight types of JUUL brand e-liquids ("Mint", "Virginia Tobacco" and "Menthol", all containing 3% nicotine at 1% and 3% (vol/vol) dilutions) and assessed for viability using a resazurin-based assay. Read More

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February 2021

The solvent and treatment regimen of sodium selenite cause its effects to vary on the radiation response of human bronchial cells from tumour and normal tissues.

Med Oncol 2020 Nov 18;37(12):115. Epub 2020 Nov 18.

Department of Radiotherapy and Radiation Oncology, University Medical Center Rostock, Suedring 75, 18059, Rostock, Germany.

Sodium selenite is often given to moderate the side effects of cancer therapy to enhance the cellular defence of non-cancerous cells. To determine whether sodium selenite during radiotherapy protects not only normal cells but also cancer cells, which would imply a reduction of the desired effect of irradiation on tumour during radiotherapy, the effect of the combined treatment of irradiation and sodium selenite was investigated. Human bronchial cells from carcinoma (A549) and normal tissue (BEAS-2B) were treated with sodium selenite and effects on growth and in combination with radiation on metabolic activity and cell cycle distribution were studied. Read More

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November 2020

Mechanoadaptive organization of stress fiber subtypes in epithelial cells under cyclic stretches and stretch release.

Sci Rep 2020 10 29;10(1):18684. Epub 2020 Oct 29.

School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju, Republic of Korea.

Cyclic stretch applied to cells induces the reorganization of stress fibers. However, the correlation between the reorganization of stress fiber subtypes and strain-dependent responses of the cytoplasm and nucleus has remained unclear. Here, we investigated the dynamic involvement of stress fiber subtypes in the orientation and elongation of cyclically stretched epithelial cells. Read More

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October 2020

Identification and characterization of two bacteriophages with lytic activity against multidrug-resistant Escherichia coli.

Virus Res 2021 01 21;291:198196. Epub 2020 Oct 21.

Center for Translational Medicine, Hubei Clinical Research Center of Parkinson's disease, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang, 441000, China. Electronic address:

Escherichia coli is an opportunistic bacterial pathogen that causes a wide range of nosocomial infections. The emergence of multidrug resistance in E. coli poses a severe threat to global health. Read More

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January 2021

Structure, Antioxidant and Anti-inflammatory Activities of the (4)- and (4)-epimers of -Carboxymethyl-L-cysteine Sulfoxide.

Pharmaceuticals (Basel) 2020 Sep 25;13(10). Epub 2020 Sep 25.

Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA.

-Carboxymethyl-L-cysteine (CMC) is an antioxidant and mucolytic commonly prescribed to patients with chronic obstructive pulmonary disease. In humans, CMC is rapidly metabolized to -carboxymethyl-L-cysteine sulfoxide (CMCO). In this study, we assessed structural and functional similarities between CMC and CMCO. Read More

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September 2020

Nucleic Acid Delivery with α-Tocopherol-Polyethyleneimine-Polyethylene Glycol Nanocarrier System.

Int J Nanomedicine 2020 11;15:6689-6703. Epub 2020 Sep 11.

School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA, USA.

Purpose: Nucleic acid-based therapies are a promising therapeutic tool. The major obstacle in their clinical translation is their efficient delivery to the desired tissue. We developed a novel nanosized delivery system composed of conjugates of α-tocopherol, polyethyleneimine, and polyethylene glycol (TPP) to deliver nucleic acids. Read More

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November 2020

Therapeutic efficacy and safety of a human fusion construct targeting the TWEAK receptor Fn14 and containing a modified granzyme B.

J Immunother Cancer 2020 09;8(2)

Experimental Therapeutics, University of Texas MD Anderson Cancer Center Division of Cancer Medicine, Houston, Texas, USA.

Background: Antibody-drug conjugates are an exceptional and useful therapeutic tool for multiple diseases, particularly for cancer treatment. We previously showed that the fusion of the serine protease granzyme B (GrB), the effector molecule or T and B cells, to a binding domain allows the controlled and effective delivery of the cytotoxic payload into the target cell. The production of these constructs induced the formation of high molecular aggregates with a potential impact on the efficacy and safety of the protein. Read More

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September 2020

Cytotoxic alkyl-quinolones mediate surface-induced virulence in Pseudomonas aeruginosa.

PLoS Pathog 2020 09 14;16(9):e1008867. Epub 2020 Sep 14.

Department of Molecular Biology, Princeton University, Princeton, NJ, Unites States of America.

Surface attachment, an early step in the colonization of multiple host environments, activates the virulence of the human pathogen P. aeruginosa. However, the downstream toxins that mediate surface-dependent P. Read More

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September 2020

Project IDentif.AI: Harnessing Artificial Intelligence to Rapidly Optimize Combination Therapy Development for Infectious Disease Intervention.

Adv Ther (Weinh) 2020 Apr 16:2000034. Epub 2020 Apr 16.

Institute for Personalized Medicine School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 China.

In 2019/2020, the emergence of coronavirus disease 2019 (COVID-19) resulted in rapid increases in infection rates as well as patient mortality. Treatment options addressing COVID-19 included drug repurposing, investigational therapies such as remdesivir, and vaccine development. Combination therapy based on drug repurposing is among the most widely pursued of these efforts. Read More

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Broad Anti-coronavirus Activity of Food and Drug Administration-Approved Drugs against SARS-CoV-2 and SARS-CoV .

J Virol 2020 10 14;94(21). Epub 2020 Oct 14.

Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China at the end of 2019 and has rapidly caused a pandemic, with over 20 million recorded COVID-19 cases in August 2020 (https://covid19.who.int/). Read More

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October 2020

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet.

Oxid Med Cell Longev 2020 30;2020:4205640. Epub 2020 Jul 30.

Department of Biological Sciences, Dong-A University, Busan 49315, Republic of Korea.

Cold atmospheric plasma (CAP) has been reported to have strong anticancer effects and . CAP has been known to induce apoptosis in most cancer cells by treatment to cells using direct and indirect treatment methods. There are many reports of apoptosis pathways induced by CAP, but for indirect treatment, there is still a lack of fundamental research on how CAP can cause apoptosis in cancer cells. Read More

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Differential adaptability between reference strains and clinical isolates of into the lung epithelium intracellular lifestyle.

Virulence 2020 12;11(1):862-876

Bacterial Infections and Antimicrobial Therapies Group, Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Science and Technology , Barcelona, Spain.

Intracellular invasion is an advantageous mechanism used by pathogens to evade host defense and antimicrobial therapy. In patients, the intracellular microbial lifestyle can lead to infection persistence and recurrence, thus worsening outcomes. Lung infections caused by , especially in cystic fibrosis (CF) patients, are often aggravated by intracellular invasion and persistence of the pathogen. Read More

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December 2020

Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells.

Front Mol Biosci 2020 30;7:116. Epub 2020 Jun 30.

GL Sciences Inc., Saitama, Japan.

Cellular volatile organic compounds (VOCs) are unique compounds whose metabolic pathways remain enigmatic. To elucidate their metabolism, we investigated the VOCs of lung cancer A549 and 2 non-cancer lung cells (HLB; HBEpC). Neutral sugars and lactate in the medium were measured by colorimetric assay. Read More

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