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A novel rosamine-based fluorescent probe for the rapid and selective detection of cysteine in BSA, water, milk, cabbage, radish, apple, and pear.

Food Chem 2021 Mar 23;356:129658. Epub 2021 Mar 23.

Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin 300387, China. Electronic address:

A novel fluorescent probe (RA), based on the rosamine skeleton bearing acrylate group, has been reasonably designed and prepared, which employed an addition-cyclization-elimination sequence reaction mechanism to detect cysteine. RA displayed rapid response to cysteine within 1.5 min, and exhibited satisfactory selectivity for cysteine over HS, glutathione (Glu), and homocysteine (Hcy), due to the formation of seven-membered lactam favored kinetically. Read More

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Design, synthesis and biological evaluation of anilide (dicarboxylic acid) shikonin esters as antitumor agents through targeting PI3K/Akt/mTOR signaling pathway.

Bioorg Chem 2021 Mar 29;111:104872. Epub 2021 Mar 29.

State Key Laboratory of Pharmaceutical Biotechnology, Institute of Plant Molecular Biology, School of Life Sciences, Nanjing University, Nanjing 210023, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

Triple-negative breast cancer (TNBC) has an unfavorable prognosis attribute to its low differentiation, rapid proliferation and high distant metastasis rate. PI3K/Akt/mTOR as an intracellular signaling pathway plays a key role in the cell proliferation, migration, invasion, metabolism and regeneration. In this work, we designed and synthesized a series of anilide (dicarboxylic acid) shikonin esters targeting PI3K/Akt/mTOR signaling pathway, and assessed their antitumor effects. Read More

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Novel 1,2,4-oxadiazole-chalcone/oxime hybrids as potential antibacterial DNA gyrase inhibitors: Design, synthesis, ADMET prediction and molecular docking study.

Bioorg Chem 2021 Apr 1;111:104885. Epub 2021 Apr 1.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sohag University, 82524 Sohag, Egypt. Electronic address:

New antibacterial drugs are urgently needed to tackle the rapid rise in multi-drug resistant bacteria. DNA gyrase is a validated target for the development of new antibacterial drugs. Thus, in the present investigation, a novel series of 1,2,4-oxadiazole-chalcone/oxime (6a-f) and (7a-f) were synthesized and characterized by IR, NMR (H and C) and elemental analyses. Read More

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Optimized BMSC-derived osteoinductive exosomes immobilized in hierarchical scaffold via lyophilization for bone repair through Bmpr2/Acvr2b competitive receptor-activated Smad pathway.

Biomaterials 2021 Mar 27;272:120718. Epub 2021 Mar 27.

Department of Oral & Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, 200011, PR China; Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China. Electronic address:

Mesenchymal stem cell-derived exosomes (MSC-exos), with its inherent capacity to modulate cellular behavior, are emerging as a novel cell-free therapy for bone regeneration. Herein, focusing on practical applying problems, the osteoinductivity of MSC-exos produced by different stem cell sources (rBMSCs/rASCs) and culture conditions (osteoinductive/common) were systematically compared to screen out an optimized osteogenic exosome (BMSC-OI-exo). Via bioinformatic analyses by miRNA microarray and in vitro pathway verification by gene silencing and miRNA transfection, we first revealed that the osteoinductivity of BMSC-OI-exo was attributed to multi-component exosomal miRNAs (let-7a-5p, let-7c-5p, miR-328a-5p and miR-31a-5p). Read More

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Microwave-assisted assembly of AgO-ZnO composite nanocones for electrochemical detection of 4-Nitrophenol and assessment of their photocatalytic activity towards degradation of 4-Nitrophenol and Methylene blue dye.

J Hazard Mater 2021 Mar 27;416:125771. Epub 2021 Mar 27.

Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University Chandigarh, 160014, India; SAIF/CIL, Panjab University Chandigarh, 160014, India. Electronic address:

4-Nitrophenol (4-NP) is an extensively utilized industrial chemical and one of major toxic water pollutant. Therefore, there is an urgent need to monitor the levels of 4-NP from environmental samples as well as its eradication are extremely important. Keeping this as a motivation, this research for the first-time reports microwave-assisted cost-effective synthesis of silver oxide (AgO)-zinc oxide (ZnO) composite nanocones (CNCs, 80-100 nm) for simultaneous electrochemical detection and photodegradation of 4-NP from aqueous solutions. Read More

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Electronic waste generation, recycling and resource recovery: Technological perspectives and trends.

J Hazard Mater 2021 Mar 16;416:125664. Epub 2021 Mar 16.

Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam; College of Medical and Health Science, Asia University, Taichung, Taiwan. Electronic address:

The growing population and increased disposal of end-of-life (EoL) electrical and electronic products have caused serious concerns to the environment and human health. Electronic waste (e-waste) is a growing problem because the quantity and the rate at which it is generated has increased exponentially in the last 5 years. The rapid changes or upgradation in technologies, IT requirements for working or learning from home during COVID-19, manufacturers releasing new electronic gadgets and devices that serves the consumers comfort and a declension in services has contributed to an increase in the e-waste or waste of electrical and electronic equipment (WEEE) generation rates. Read More

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Injury Severity, Arrival Physiology, Coagulopathy, and Outcomes Among the Youngest Trauma Patients.

J Surg Res 2021 Apr 7;264:236-241. Epub 2021 Apr 7.

Department of Pediatric Surgery, Houston, Texas; Center for Surgical Trials and Evidence-based Practice, the McGovern Medical School at The University of Texas-Houston, Houston, Texas. Electronic address:

Background: Although physiologic differences exist between younger and older children, pediatric trauma analyses are weighted toward older patients. Trauma-induced coagulopathy, determined by rapid thrombelastography (rTEG), is a predictor of outcome in trauma patients, but the significance of rTEG values among very young trauma patients remains unknown. Our objective was to identify the prehospital or physiologic factors, including rTEG values, that were associated with mortality in trauma patients younger than 5 y old. Read More

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Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces.

J Colloid Interface Sci 2021 Mar 11;596:64-74. Epub 2021 Mar 11.

Department of Optics, Complutense University of Madrid, E-28037 Madrid, Spain. Electronic address:

In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: (i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and (ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. Read More

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Development and clinical validation of loop-mediated isothermal amplification (LAMP) assay to diagnose high HBV DNA levels in resource-limited settings.

Clin Microbiol Infect 2021 Apr 7. Epub 2021 Apr 7.

Unité d'Epidémiologie des Maladies Émergentes, Institut Pasteur, Paris, France. Electronic address:

Objectives: A massive scale-up of testing and treatment is indicated to globally eliminate hepatitis B virus (HBV) infection. However, access to a polymerase chain reaction (PCR), a key test to quantify HBV DNA levels and determine treatment eligibility, is limited in resource-limited countries. We developed and evaluated loop-mediated isothermal amplification (LAMP) assay to diagnose clinically important HBV DNA thresholds defined by WHO (≥20,000 and ≥200,000 IU/mL). Read More

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Challenges of Automation and Scale: Bioinformatics and the Evaluation of Proteins to Support Genetically Modified Product Safety Assessments.

J Invertebr Pathol 2021 Apr 7:107587. Epub 2021 Apr 7.

Bayer Crop Science, 700 Chesterfield Pkwy West, Chesterfield, MO 63017.

Bioinformatic analyses of protein sequences play an important role in the discovery and subsequent safety assessment of insect control proteins in Genetically Modified (GM) crops. Due to the rapid adoption of high-throughput sequencing methods over the last decade, the number of protein sequences in GenBank and other public databases has increased dramatically. Many of these protein sequences are the product of whole genome sequencing efforts, coupled with automated protein sequence prediction and annotation pipelines. Read More

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A Diagnostic randomised controlled trial to study the impact of Rapid diagnostic tests in patients with Acute febrile illness when compared to Conventional diagnostics (DRACO study).

J Infect 2021 Apr 7. Epub 2021 Apr 7.

Department of Infectious Diseases, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104; Manipal Center for Infectious Diseases, Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104.

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Voxelotor in adolescents and adults with sickle cell disease (HOPE): long-term follow-up results of an international, randomised, double-blind, placebo-controlled, phase 3 trial.

Lancet Haematol 2021 Apr 7. Epub 2021 Apr 7.

UCSF Benioff Children's Hospital Oakland, Oakland, CA, USA.

Background: For decades, patients with sickle cell disease have had only a limited number of therapies available. In 2019, voxelotor (1500 mg), an oral once-daily sickle haemoglobin polymerisation inhibitor, was approved in the USA for the treatment of sickle cell disease in patients aged 12 years and older on the basis of HOPE trial data. To further describe the applicability of voxelotor as a treatment for this chronic illness, we report the long-term efficacy and safety of this drug at 72 weeks of treatment; the conclusion of the placebo-controlled HOPE trial. Read More

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Heart rate detection by Fitbit ChargeHR : A validation study versus portable polysomnography.

J Sleep Res 2021 Apr 10:e13346. Epub 2021 Apr 10.

Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Pisa, Italy.

Consumer "Smartbands" can collect physiological parameters, such as heart rate (HR), continuously across the sleep-wake cycle. Nevertheless, the quality of HR data detected by such devices and their place in the research and clinical field is debatable, as they are rarely rigorously validated. The objective of the present study was to investigate the reliability of pulse photoplethysmographic detection by the Fitbit ChargeHR (FBCHR, Fitbit Inc. Read More

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The association of rocuronium dosing and first-attempt intubation success in adult emergency department patients.

CJEM 2021 Apr 10. Epub 2021 Apr 10.

Department of Emergency Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Background: The recommended rocuronium dose for rapid sequence intubation is 1.0 mg/kg; however, the optimal dose for emergency airway management is not clear. We assessed the relationship between rocuronium dose and first-attempt success among emergency department (ED) patients undergoing rapid sequence intubation. Read More

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Towards a green economic policy framework in China: role of green investment in fostering clean energy consumption and environmental sustainability.

Environ Sci Pollut Res Int 2021 Apr 10. Epub 2021 Apr 10.

School of Economics & Management, China University of Geosciences, Wuhan, China.

Recently, China has relished rapid green investment, and its influence on clean energy consumption and environment is substantial. Therefore, this study scrutinizes the effects of green investment on clean energy consumption and CO2 emissions in China by using autoregressive distributed lag model (ARDL) approach over time from 1998 to 2019. The results show that green investment tends to have a positive effect on clean energy consumption in China in the long run. Read More

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Associations between sleep duration patterns and cognitive decline trajectories in older Chinese adults.

Aging Clin Exp Res 2021 Apr 10. Epub 2021 Apr 10.

Department of Preventive Medicine, North Sichuan Medical College, Fujiang Road, Shunqing, Nanchong, 234, Sichuan, China.

Background: Abnormal sleep duration has been identified as a risk factor for cognitive decline. However, most studies have measured sleep duration and cognitive function at a single time point, while both tend to decrease with age.

Aims: This study aimed to investigate the association between changes in sleep duration patterns and cognitive trajectories in older adults. Read More

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Does Inhaled Methoxyflurane Implement Fast and Efficient Pain Management in Trauma Patients? A Systematic Review and Meta-Analysis.

Pain Ther 2021 Apr 10. Epub 2021 Apr 10.

Hospital of Chengdu University of Traditional Chinese Medicine, No. 39 Shi-er-qiao Road, Chengdu, 610072, Sichuan Province, China.

Introduction: Evidence on the use of inhaled methoxyflurane in the management of trauma pain is conflicting and obfuscated. This study aimed to determine the efficacy and safety of inhaled methoxyflurane for trauma pain on the basis of published randomized controlled trials (RCTs).

Methods: RCTs assessing the efficacy of methoxyflurane in adults or adolescents with acute trauma pain published in PubMed, Web of Science, Embase, Cochrane Library, and Google Scholar were searched. Read More

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Multicenter evaluation of the VITEK MS matrix-assisted laser desorption/ionization-time of flight mass spectrometry system for identification of bacteria, including Brucella, and yeasts.

Eur J Clin Microbiol Infect Dis 2021 Apr 10. Epub 2021 Apr 10.

R&D Microbiology, bioMérieux, Inc., St. Louis, MO, USA.

The use of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry has proven to be rapid and accurate for the majority of clinical isolates. Some gaps remain concerning rare, emerging, or highly pathogenic species, showing the need to continuously expand the databases. In this multicenter study, we evaluated the accuracy of the VITEK MS v3. Read More

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Deciphering Genetic Variants of Warfarin Metabolism in Children With Ventricular Assist Devices.

Pediatr Cardiol 2021 Apr 10. Epub 2021 Apr 10.

Heart Failure, Transplant and Mechanical Assistance Program Unit, Bambino Gesù Children Hospital, Rome, Italy.

Warfarin is prescribed in patients with ventricular assist devices (VADs). Dosage depends on several factors including the underlying genotype. These include polymorphisms of genes encoding cytochrome P450 enzymes, the main ones being CYP2C9, VKORC1, and CYP4F2. Read More

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Vestiges of the bacterial signal recognition particle-based protein targeting in mitochondria.

Mol Biol Evol 2021 Apr 10. Epub 2021 Apr 10.

Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.

The main bacterial pathway for inserting proteins into the plasma membrane relies on the signal recognition particle (SRP), composed of the Ffh protein and an associated RNA component, and the SRP-docking protein FtsY. Eukaryotes use an equivalent system of archaeal origin to deliver proteins into the endoplasmic reticulum, whereas a bacteria-derived SRP and FtsY function in the plastid. Here we report on the presence of homologs of the bacterial Ffh and FtsY proteins in various unrelated plastid-lacking unicellular eukaryotes, namely Heterolobosea, Alveida, Goniomonas, and Hemimastigophora. Read More

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Efficacy of one-dose intramuscular rabies vaccine as pre-exposure prophylaxis in travellers.

J Travel Med 2021 Apr 10. Epub 2021 Apr 10.

Research School of Population Health, Australian National University, Canberra, Australia.

Background: Current guidelines for rabies pre-exposure prophylaxis (PrEP) recommend multiple vaccine doses. Travellers sometimes present for pre-travel consultation with insufficient time to complete standard PrEP schedules. We investigated the efficacy of one-dose intramuscular (IM) vaccine in priming the immune system (as PrEP) by measuring antibody response to simulated post-exposure prophylaxis (PEP). Read More

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Cell membrane mechanics and mechanosensory transduction.

Curr Top Membr 2020 ;86:83-141

Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Sydney, Australia; St. Vincent's Clinical School, University of New South Wales, Sydney, Australia.

The rapid progress in mechanobiology has brought together many scientific and engineering disciplines to work hand in hand toward better understanding of the role that mechanical force plays in functioning and evolution of different forms of life. New tools designed by engineers helped to develop new methods and techniques for investigation of mechanical properties of biological cells and tissues. This multidisciplinary approach made it clear that cell mechanics is tightly linked to intracellular signaling pathways, which directly regulate gene expression in response to mechanical stimuli originating outside or inside the cells. Read More

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January 2020

COVID-19-Related Occupational Burnout and Moral Distress among Nurses: A Rapid Scoping Review.

Nurs Leadersh (Tor Ont) 2021 Mar;34(1):7-19

Policy Advisor, Policy and Government Relations, Canadian Nurses Association, Ottawa, ON.

Background: The COVID-19 pandemic is placing unprecedented pressure on a nursing workforce that is already under considerable mental strain due to an overloaded system. Convergent evidence from the current and previous pandemics indicates that nurses experience the highest levels of psychological distress compared with other health professionals. Nurse leaders face particular challenges in mitigating risk and supporting nursing staff to negotiate moral distress and fatigue during large-scale, sustained crises. Read More

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Externally added cystatin C reduces growth of A375 melanoma cells by increasing cell cycle time.

FEBS Open Bio 2021 Apr 10. Epub 2021 Apr 10.

Division of Clinical Chemistry & Pharmacology, Department of Laboratory Medicine, Lund University, Lund, Sweden.

Some secreted cysteine protease inhibitors of the cystatin family appear to affect intracellular proteolysis and growth of human cells, as a result of internalization. Here, we studied the effects of external addition of the most abundant human cystatin, cystatin C, on viability and proliferation of cancer cells in culture. A dose-dependent decrease of viable cells was seen for A375 melanoma, MCF-7 breast cancer and PC-3 prostate cancer cells cultured in 1-5 μM cystatin C after 24 h. Read More

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Remodeling of Arctic char (Salvelinus alpinus) lipidome under a stimulated scenario of Arctic warming.

Glob Chang Biol 2021 Apr 10. Epub 2021 Apr 10.

Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada.

Arctic warming associated with global climate change poses a significant threat to populations of wildlife in the Arctic. Since lipids play a vital role in adaptation of organisms to variations in temperature, high-resolution mass spectrometry based lipidomics can provide insights into adaptive responses of organisms to a warmer environment in the Arctic and help to illustrate potential novel roles of lipids in the process of thermal adaption. In this study, we studied an ecologically and economically important species-Arctic char (Salvelinus alpinus), with a detailed multi-tissue analysis of the lipidome in response to chronic shifts in temperature by use of a validated lipidomics workflow. Read More

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Impact of analysis technique on our understanding of the natural history of labour: a simulation study.

BJOG 2021 Apr 9. Epub 2021 Apr 9.

The University of Sydney, School of Public Health, Faculty of Medicine and Health, Sydney, NSW, Australia.

Objective: To evaluate the discrepancy between historical and more recent descriptions of the first stage of labour by testing whether the statistical techniques used recently (repeated measures polynomial and interval censored regression) were appropriate for detection of periods of rapid acceleration of cervical dilatation as might occur at the time of transition from a latent to an active phase of labour.

Design And Setting: A simulation study using regression techniques.

Sample: We created a simulated dataset for 500,000 labours with clearly defined latent and active phases using the parameters described by Friedman. Read More

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Development and validation of a simultaneous analytical method for non-steroidal therapeutic compounds in cosmetics using LC-MS/MS.

J Sep Sci 2021 Apr 9. Epub 2021 Apr 9.

Center for Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Cheongju-si, Chungcheongbuk-do, Republic of Korea.

Atopic dermatitis is a typical chronic inflammatory skin disease that affects all age groups and requires basic skin care for treatment. Anti-inflammatory and antiallergy steroids are the most frequently used treatments but they are limited due to their side effects caused by a weakening of the immune system. Many consumers focus on performance as a criterion for selecting cosmetics. Read More

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Simple and sensitive detection of doxyribonucleic acid using RecA- GFP fusion protein-DNA filament as probe.

Luminescence 2021 Apr 10. Epub 2021 Apr 10.

Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, China.

A simple, rapid and highly sensitive method for the detection of double-stranded DNA (dsDNA) was developed using a novel fluorescence probe which was composed of RecA-GFP fusion protein and specific recognition of ssDNA complexes (RecA-GFP-DNA filament). The RecA-GFP fusion protein not only has strong fluorescence, but also can occur to homologous recombination. In the presence of the target dsDNA, the complementary ssDNA of RecA-GFP-DNA filaments invaded into one end of the dsDNA chain, meanwhile, the other end of ssDNA dissociated RecA-GFP filaments. Read More

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Hollow Electrode Capillary Plasma Ionization Source for Rapid On-line Detection of Gaseous Samples.

Rapid Commun Mass Spectrom 2021 Apr 9:e9099. Epub 2021 Apr 9.

Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, Ningbo University, Ningbo, 315211, China.

Rationale: Gas Chromatography Mass Spectrometry (GC-MS) with electron ionization (EI)is the most widely used analysis technique of gaseous samples, but it may be time-consuming for on-line monitoring of mixtures whose concentrations relatively change rapidly. On the other hand, current ionization methods, such as chemical ionization (CI) and proton transfer reaction (PTR), also have some disadvantages such as selectivity. Appropriate soft ionization sources therefore are searched for rapid on-line detection. Read More

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An ultrafast and flexible LC-MS/MS system paves the way for machine learning driven in vivo sample processing and data evaluation in early drug discovery.

Rapid Commun Mass Spectrom 2021 Apr 9:e9096. Epub 2021 Apr 9.

Boehringer Ingelheim Pharma GmbH & Co. KG, Drug Discovery Sciences, Birkendorfer Strasse 65, 88397, Biberach an der Riss, Germany.

Rationale: Low speed and low flexibility of most LC-MS/MS approaches in early drug discovery delay sample analysis from routine in vivo studies within the same day. A high-throughput platform for the rapid quantification of drug compounds in various in vivo assays was developed and established in routine bioanalysis.

Methods: Automated selection of an efficient and adequate LC method was realized by autonomous sample qualification for ultrafast batch gradients (9 s/sample) or for fast linear gradients (45 s/sample) if samples required chromatography. Read More

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