4,798 results match your criteria quantitative principle


Two-dimensional transition metal borides as high activity and selectivity catalysts for ammonia synthesis.

Nanoscale 2021 Oct 19. Epub 2021 Oct 19.

School of Physics, State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.

In comparison to defect/doping induced activity in materials, transition metal borides with exposed metal atoms, large specific surface area, and high active site density show advantages as durable and efficient catalysts for specific electrochemical reactions. In this work, ReB for N reduction reaction (NRR) for ammonia (NH) with a record-low limiting potential of = -0.05 V and high Faraday efficiency (FE) of 100% is screened out from a new class of TMB. Read More

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October 2021

Uncertainty Quantification of Regional Cardiac Tissue Properties in Arrhythmogenic Cardiomyopathy Using Adaptive Multiple Importance Sampling.

Front Physiol 2021 30;12:738926. Epub 2021 Sep 30.

Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, Netherlands.

Computational models of the cardiovascular system are widely used to simulate cardiac (dys)function. Personalization of such models for patient-specific simulation of cardiac function remains challenging. Measurement uncertainty affects accuracy of parameter estimations. Read More

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September 2021

Toward Predicting Impact of Common Genetic Variants on Schizophrenia Clinical Responses With Antipsychotics: A Quantitative System Pharmacology Study.

Front Neurosci 2021 29;15:738903. Epub 2021 Sep 29.

In Silico Biosciences, Berwyn, PA, United States.

CNS disorders are lagging behind other indications in implementing genotype-dependent treatment algorithms for personalized medicine. This report uses a biophysically realistic computer model of an associative and dorsal motor cortico-striatal-thalamo-cortical loop and a working memory cortical model to investigate the pharmacodynamic effects of COMTVal158Met rs4680, 5-HTTLPR rs 25531 s/L and D2DRTaq1A1 genotypes on the clinical response of 7 antipsychotics. The effect of the genotypes on dopamine and serotonin dynamics and the level of target exposure for the drugs was calibrated from PET displacement studies. Read More

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September 2021

Use of Peptide Microarrays for Fast and Informative Profiling of Therapeutic Antibody Formulation Conditions.

Mol Pharm 2021 Oct 18. Epub 2021 Oct 18.

School of Biological Sciences, Faculty of Biology, Medicine, and Health, University of Manchester, Manchester M13 9PT, United Kingdom.

Methods to optimize the solution behavior of therapeutic proteins are frequently time-consuming, provide limited information, and often use milligram quantities of material. Here, we present a simple, versatile method that provides valuable information to guide the identification and comparison of formulation conditions for, in principle, any biopharmaceutical drug. The subject protein is incubated with a designed synthetic peptide microarray; the extent of binding to each peptide is dependent on the solution conditions. Read More

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October 2021

Making a science out of preanalytics: An analytical method to determine optimal tissue fixation in real-time.

PLoS One 2021 14;16(10):e0258495. Epub 2021 Oct 14.

Roche Tissue Diagnostics (Ventana Medical Systems, Inc.), Tucson, Arizona, United States of America.

Modern histopathology is built on the cornerstone principle of tissue fixation, however there are currently no analytical methods of detecting fixation and as a result, in clinical practice fixation is highly variable and a persistent source of error. We have previously shown that immersion in cold formalin followed by heated formalin is beneficial for preservation of histomorphology and have combined two-temperature fixation with ultra-sensitive acoustic monitoring technology that can actively detect formalin diffusing into a tissue. Here we expand on our previous work by developing a predictive statistical model to determine when a tissue is properly diffused based on the real-time acoustic signal. Read More

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October 2021

Understanding Inflammation-associated Ophthalmic Pathologies: A Novel 3D Co-culture Model of Monocyte-myofibroblast Immunomodulation.

Ocul Immunol Inflamm 2021 Oct 14:1-12. Epub 2021 Oct 14.

Department of Biochemistry, Western University, London, Canada.

Inflammation is associated with, and may be causal of, a variety of ophthalmic pathologies. These pathologies are currently difficult to model because they involve complex interactions between the innate immune system, stromal cells, and other cells that normally maintain ocular tissue homeostasis. Using transscleral drainage channel fibrosis after glaucoma surgery as an example of inflammation-associated ocular fibrosis, we have assessed a simple but novel 3D cell culture system designed to reveal the immunomodulatory impacts of ocular connective tissue cells on monocytes, a major cellular component of the circulating immune system. Read More

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October 2021

Morphometric study of the testis in sheep embryos using unbiased design-based stereology.

Anat Histol Embryol 2021 Oct 13. Epub 2021 Oct 13.

Department of Veterinary Medical Sciences (UNI EN ISO 9001:2008), University of Bologna, Bologna, Italy.

Sheep have been used as translational models of human postnatal testicular development. However, the morphometric features of the normal developing testis in sheep embryos have not been previously investigated using stereology. The objective of the present work was to establish normal quantitative parameters for fetal testicular tissue components in sheep, using unbiased design-based stereological methods. Read More

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October 2021

A simple molecular method to identify and quantify genera of gastrointestinal nematodes of cattle.

Parasitol Res 2021 Oct 14. Epub 2021 Oct 14.

Technical Services, Merck Animal Health, Madison, NJ, USA.

Classic approaches for antemortem identification of gastrointestinal nematodes (GIN) require coproculture of eggs and morphological examination. While adequate for diagnosis, many PCR techniques cannot easily quantify mixed infections without controls and/or standard curves. Herein, we developed a simple and rapid test for differentiating and quantifying mixed infections of GIN using PCR products separated by capillary electrophoresis. Read More

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October 2021

High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array.

Genomics Inform 2021 Sep 30;19(3):e34. Epub 2021 Sep 30.

Department of Bioconvergence Engineering, Dankook University, Yongin 16890, Korea.

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. Read More

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September 2021

Elucidating the estrogen-like effects and biocompatibility of the herbal components in the Qing' E formula.

J Ethnopharmacol 2021 Oct 9:114735. Epub 2021 Oct 9.

The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China.

Ethnopharmacological Relevance: The Qing' E Formula (QEF) is a compound preparation that was originally recorded in the 'Prescriptions of the Bureau of Taiping People's Welfare Pharmacy' during the Song Dynasty (10th century CE). It consists of four Chinese medicinal herbs, Eucommiae Cortex (Eucommia ulmoides), Psoraleae Fructus (Psoralea corylifolium), Juglandis Semen (Juglans regia), and Garlic Rhizoma. According to traditional Chinese medicine (TCM), QEF has the ability to tonify the kidney and strengthen muscle and bone. Read More

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October 2021

A high-throughput, open-space and reusable microfluidic chip for combinational drug screening on tumor spheroids.

Lab Chip 2021 10 12;21(20):3924-3932. Epub 2021 Oct 12.

Department of Biology, Southern University of Science and Technology, Shenzhen, 518055, Guangdong, China.

Screening drug combinations using tumor spheroids can play a vital role in the development of disease treatment and personalized medicine. However, current studies focus on drug gradients or combinations of two drugs in most cases, and it is difficult to find complex therapeutic combinations involving more drugs. The use of design-of-experiment (DOE) microfluidics is a potential strategy to study this area systematically. Read More

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October 2021

Predictably Engineering the Viscoelastic Behavior of Dynamic Hydrogels via Correlation with Molecular Parameters.

Adv Mater 2021 Oct 11:e2104460. Epub 2021 Oct 11.

Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.

Rational design of dynamic hydrogels with desirable viscoelastic behaviors relies on an in-depth understanding of the principles correlating molecular parameters and macroscopic properties. To quantitatively elucidate such principles, a series of dynamic covalent hydrogels crosslinked via hydrazone bonds is designed. The exchange rate of the hydrazone bond is tuned by varying the concentration of an organic catalyst, while maintaining the crosslinking density unchanged. Read More

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October 2021

The breath print represents a novel biomarker of malnutrition in pulmonary arterial hypertension: a proof-of-concept study.

JPEN J Parenter Enteral Nutr 2021 Oct 11. Epub 2021 Oct 11.

Respiratory Institute, Cleveland Clinic, Cleveland, OH, United States.

Background: The breath print is a quantitative measurement of molecules in exhaled breath and represents a new frontier for biomarker identification. It is unknown whether this state-of-the-art, noninvasive method can detect malnutrition. We hypothesize that individuals with malnutrition will present with a distinguishable breath print. Read More

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October 2021

Single-shot higher-order transport-of-intensity quantitative phase imaging using deep learning.

Appl Opt 2021 Oct;60(28):8802-8808

Single-shot higher-order transport-of-intensity quantitative phase imaging (SHOT-QPI) is proposed to realize simple, in-line, scanless, and single-shot QPI. However, the light-use efficiency of SHOT-QPI is low because of the use of an amplitude-type computer-generated hologram (CGH). Although a phase-type CGH overcomes the problem, the accuracy of the measured phase is degraded owing to distortion of the defocused intensity distributions, which is caused by a quantization error of the CGH. Read More

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October 2021

Propargylic -adenosyl-l-selenomethionine: A Chemical Tool for Methylome Analysis.

Acc Chem Res 2021 Oct 6;54(20):3818-3827. Epub 2021 Oct 6.

Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

ConspectusDevising synthetic strategies to construct a covalent bond is a common research topic among synthetic chemists. A key driver of success is the high tunability of the conditions, including catalysts, reagents, solvents, and reaction temperature. Such flexibility of synthetic operations has allowed for the rapid exploration of a myriad of artificial synthetic transformations in recent decades. Read More

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October 2021

Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.

Cell Rep 2021 Oct;37(1):109797

Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., 44227 Dortmund, Germany; Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, 1090 Wien, Austria. Electronic address:

Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Read More

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October 2021

Quantitative determination of new anti-inflammatory drug indomenthyl and its metabolite in rabbit plasma by HPLC-MS/MS.

Anal Methods 2021 Oct 5. Epub 2021 Oct 5.

National Research Tomsk State University, FSAEI HE, 36, Lenin Ave., Tomsk, Russia, 634050.

: Indomenthyl is an innovative anti-inflammatory drug with a high analgesic activity. Indomenthyl releases indomethacin under the influence of neutrophil esterases in the inflammation focus. /: This research is aimed at developing a highly sensitive method for the quantitative determination of indomenthyl and its active metabolite indomethacin in rabbit plasma by HPLC-MS/MS. Read More

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October 2021

Gold nanoparticle based plasmonic sensing for the detection of SARS-CoV-2 nucleocapsid proteins.

Biosens Bioelectron 2021 Sep 28;195:113669. Epub 2021 Sep 28.

Department of Mechanical Engineering, University of California, Berkeley, CA, USA; Berkeley Sensor and Actuation Center (BSAC), Berkeley, CA, USA.

An inexpensive virus detection scheme with high sensitivity and specificity is desirable for broad applications such as the COVID-19 virus. In this article, we introduce the localized surface plasmon resonance (LSPR) principle on the aggregation of antigen-coated gold nanoparticles (GNPs) to detect SARS-CoV-2 Nucleocapsid (N) proteins. Experiments show this technique can produce results observable by the naked eye in 5 min with a LOD (Limits of Detection) of 150 ng/ml for the N proteins. Read More

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September 2021

Main Descriptors to correlate structures with performances of electrocatalysts.

Angew Chem Int Ed Engl 2021 Sep 29. Epub 2021 Sep 29.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Zhongshan Road 457, 116023, Dalian, CHINA.

Hydrogen/oxygen redox catalysis, which involves the hydrogen evolution reaction (HER) and hydrogen oxidation reaction (HOR) and oxygen reduction reaction (ORR) and oxygen evolution reaction (OER), respectively, is crucial in determining the electrochemical performance of energy conversion and storage devices, such as water electrolysis, fuel cells and metal-air batteries. Traditional trial and error approaches to search for catalysts with high activity and stability are typically tedious and inefficient. The progress from these approaches to the rational design principle of catalysts is of great significance. Read More

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September 2021

HPV Sequencing Facilitates Ultrasensitive Detection of HPV Circulating Tumor DNA.

Clin Cancer Res 2021 Sep 27. Epub 2021 Sep 27.

Department of Radiation Oncology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.

Purpose: Human papillomavirus (HPV) DNA offers a convenient circulating tumor DNA (ctDNA) marker for HPV-associated malignancies, but current methods, such as digital PCR (dPCR), provide insufficient accuracy for clinical applications in patients with low disease burden. We asked whether a next-generation sequencing approach, HPV sequencing (HPV-seq), could provide quantitative and qualitative assessment of HPV ctDNA in low disease burden settings.

Experimental Design: We conducted preclinical technical validation studies on HPV-seq and applied it retrospectively to a prospective multicenter cohort of patients with locally advanced cervix cancer (NCT02388698) and a cohort of patients with oropharynx cancer. Read More

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September 2021

Effects of Interval Exercise Training on Serum Biochemistry and Bone Mineral Density in Dogs.

Animals (Basel) 2021 Aug 28;11(9). Epub 2021 Aug 28.

Department of Physical Education, College of Performing Arts and Sport, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea.

Exercise has been suggested as a powerful intervention for health care and fitness management in humans; however, few studies have demonstrated the benefits of exercise training in dogs. The purpose of this study was to examine the effects of exercise training on heart rate (HR), bone mineral density (BMD), muscle volume (MV), and hematological and serum biomarkers in dogs. Six healthy beagles completed the interval treadmill exercise, developed on the basis of the FITT principle, two times a week for 12 weeks. Read More

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A microfluidic system for precisely reproducing physiological blood pressure and wall shear stress to endothelial cells.

Analyst 2021 Sep 27;146(19):5913-5922. Epub 2021 Sep 27.

School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, No. 2, Linggong Rd., Dalian 116024, Liaoning Province, China.

To reproduce hemodynamic stress microenvironments of endothelial cells is of vital significance, by which one could exploit the quantitative impact of hemodynamic stresses on endothelial function and seek innovative approaches to prevent circulatory system diseases. Although microfluidic technology has been regarded as an effective method to create physiological microenvironments, a microfluidic system to precisely reproduce physiological arterial hemodynamic stress microenvironments has not been reported yet. In this paper, a novel microfluidic chip consisting of a cell culture chamber with on-chip afterload components designed by the principle of input impedance to mimic the global hemodynamic behaviors is proposed. Read More

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September 2021

Polymer solubility in ionic liquids: dominated by hydrogen bonding.

Phys Chem Chem Phys 2021 Oct 6;23(38):21893-21900. Epub 2021 Oct 6.

CAS Key Laboratory of Engineering Plastics, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100190, China.

Polymer solubility in ionic liquids (ILs) cannot be predicted by the solubility parameter approach based on the "like dissolves like" principle. According to the Kamlet-Abraham-Taft (KAT) multi-parameter polarity scale, ILs can be categorized on the basis of hydrogen-bond acidity or basicity ones. The experimental observations, that acidic ILs easily dissolve basic polymers and basic ILs dissolve acidic polymers, reflect the complementary nature of hydrogen-bonding interactions. Read More

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October 2021

Looking for more reliable biomarkers in breast cancer: Comparison between routine methods and RT-qPCR.

PLoS One 2021 23;16(9):e0255580. Epub 2021 Sep 23.

Department of Medicine and Surgery, Section of Anatomic Pathology and Histology, Medical School, University of Perugia, Perugia, Italy.

Purpose: Decades of quality control efforts have raised the standards of immunohistochemistry (IHC), the principle method used for biomarker testing in breast cancer; however, computational pathology and reverse transcription quantitative PCR (RT-qPCR) may also hold promise for additional substantial improvements.

Methods: Herein, we investigated discrepancies in the assessment of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and marker of proliferation Ki67 comparing routinely obtained IHC (and FISH) data (ORI) with the results of manual (REV) and semi-automated (DIA) re-evaluation of the original IHC slides and then with RNA expression data from the same tissue block using the MammaTyper® (MT) gene expression assay.

Results: Correlation for ER and PR was high between ORI IHC and the other three study methods (REV, DIA and RT-qPCR). Read More

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September 2021

Educational Response to a Student with Psychosis at the Secondary Level: A Non-Experimental Single-Case Study.

Eur J Investig Health Psychol Educ 2020 Nov 24;10(4):1080-1094. Epub 2020 Nov 24.

Department of Evolutionary and Educational Psychology, University of Murcia, 30100 Murcia, Spain.

Students with psychosis in school within the ordinary education system are a reality in the classroom. To study their correct adaptation at school, it is necessary to consider numerous factors such as the personal characteristics of the student, environmental variables, educational measures put in place as well as emotional and cognitive aspects. The aim of this research was to monitor the teaching-learning process of a student diagnosed with psychosis and enrolled in a public school at the secondary level in the ordinary modality with support during an academic year, with the usual resources provided by a guidance department to assess the impact of the educational measures and plans on his emotional and academic fields. Read More

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November 2020

Mass Spectrometric Biosensing: A Powerful Approach for Multiplexed Analysis of Clinical Biomolecules.

ACS Sens 2021 Sep 16. Epub 2021 Sep 16.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Rapid and sensitive detection of clinical biomolecules in a multiplexed fashion is of great importance for accurate diagnosis of diseases. Mass spectrometric (MS) approaches are exceptionally suitable for clinical analysis due to its high throughput, high sensitivity, and reliable qualitative and quantitative capabilities. To break through the bottleneck of MS technique for detecting high-molecular-weight substances with low ionization efficiency, the concept of mass spectrometric biosensing has been put forward by adopting mass spectrometric chips to recognize the targets and mass spectrometry to detect the signals switched by the recognition. Read More

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September 2021

NHS-Ester Tandem Labeling in One Pot Enables 48-Plex Quantitative Proteomics.

Anal Chem 2021 09 16;93(38):12827-12832. Epub 2021 Sep 16.

Department of Biochemistry and Biomedical Sciences, McMaster University, Michael G. DeGroote Centre for Learning and Centre, Room 5033, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada.

Stable-isotope labeling strategies are extensively used for multiplex quantitative proteomics. Hybrid-isotope labeling strategies that combine the use of isotopic mass difference labeling and isobaric tags can greatly increase sample multiplexity. In this work, we present a novel hybrid-isotope labeling approach that we termed NHS-ester tandem labeling in one pot (NETLOP). Read More

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September 2021

Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT.

Elife 2021 09 16;10. Epub 2021 Sep 16.

Division of Experimental Pathology, Department of Pathology, Pennsylvania State University College of Medicine, Hershey, United States.

We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. Read More

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September 2021

A functional peptide-mediated colorimetric assay for mercury ion based on dual-modified gold nanoparticles.

Anal Biochem 2021 Oct 10;631:114369. Epub 2021 Sep 10.

The Centre of Analysis and Measurement of Wuhan University, Wuhan University, Wuhan, 430072, PR China. Electronic address:

In the work, a rapid and accurate biosensor for mercury ions (Hg) was constructed, with which aggregation of dual-modified (DGPFHR- and CALNN-) gold nanoparticles (D/C-AuNPs) could be triggered by the high specificity of peptides to Hg. The given peptide DGPFHR possesses great capability of capturing Hg, accompanied by the conformational folding. Under the circumstances, D/C-AuNPs were employed as the detection probes to accomplish the quantitative analysis of Hg. Read More

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October 2021

Plasma metagenomic sequencing to detect and quantify bacterial DNA in ICU patients suspected of sepsis: a proof-of-principle study.

J Trauma Acute Care Surg 2021 Sep 10. Epub 2021 Sep 10.

Center for Noninvasive Diagnostics, Translational Genomics Research Institute, Phoenix, AZ, USA. Department of Surgery, University of Arizona, Tucson, AZ, USA. Department of Surgery, Brigham and Women's Hospital, Boston, MA, USA. Northern Arizona University, Flagstaff, AZ, USA.

Introduction: Timely recognition of sepsis and identification of pathogens can improve outcomes in critical care patients but microbial cultures have low accuracy and long turnaround times. In this proof-of-principle study, we describe metagenomic sequencing and analysis of non-human DNA in plasma. We hypothesized that quantitative analysis of bacterial DNA (bDNA) levels in plasma can enable detection and monitoring of pathogens. Read More

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September 2021