J Trauma Acute Care Surg 2021 Sep 10. Epub 2021 Sep 10.
Center for Noninvasive Diagnostics, Translational Genomics Research Institute, Phoenix, AZ, USA. Department of Surgery, University of Arizona, Tucson, AZ, USA. Department of Surgery, Brigham and Women's Hospital, Boston, MA, USA. Northern Arizona University, Flagstaff, AZ, USA.
Introduction: Timely recognition of sepsis and identification of pathogens can improve outcomes in critical care patients but microbial cultures have low accuracy and long turnaround times. In this proof-of-principle study, we describe metagenomic sequencing and analysis of non-human DNA in plasma. We hypothesized that quantitative analysis of bacterial DNA (bDNA) levels in plasma can enable detection and monitoring of pathogens. Read More