42,594 results match your criteria quantitative phase

Long Noncoding RNA GAS5 Targeting miR-221-3p/Cyclin-Dependent Kinase Inhibitor 2B Axis Regulates Follicular Thyroid Carcinoma Cell Cycle and Proliferation.

Pathobiology 2021 Jun 15:1-12. Epub 2021 Jun 15.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, China.

Introduction: Follicular thyroid carcinoma (FTC) is more aggressive than the most common papillary thyroid carcinoma (PTC). However, the current research on FTC is less than PTC. Here, we investigated the effects of long noncoding RNA (lncRNA) GAS5 and miR-221-3p in FTC. Read More

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Raman and quantitative phase imaging allow morpho-molecular recognition of malignancy and stages of B-cell acute lymphoblastic leukemia.

Biosens Bioelectron 2021 Jun 12;190:113403. Epub 2021 Jun 12.

Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; Department of Oncology, Johns Hopkins University, Baltimore, MD, 21287, USA. Electronic address:

Acute lymphoblastic leukemia (ALL) is one of the most common malignancies that account for nearly one-third of all pediatric cancers. The current diagnostic assays are time-consuming, labor-intensive, and require expensive reagents. Here, we report a label-free approach featuring diffraction phase imaging and Raman microscopy that can retrieve both morphological and molecular attributes for label-free optical phenotyping of individual B cells. Read More

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Quantitative analysis of the biomechanical response of semicircular canals and nystagmus under different head positions.

Hear Res 2021 Jun 6;407:108282. Epub 2021 Jun 6.

School of Information and Communication Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address:

The semicircular canals (SCCs) in the vestibular system can sense angular motion of the head, which performs a crucial role in maintaining the human's sense of balance. The different spatial orientations of the head affect the response of human SCCs to rotational movement. In this study, we combined the numerical model of bilateral human SCCs with vestibulo-ocular reflex experiments, and quantitatively investigated the responses of SCCs to constant angular acceleration when the head was in different left-leaning positions, including the head tilted 0°, 15°, 30°, 45°, 60°, 70°, 80°, and 90° to the left. Read More

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Development and psychometric properties of the Nursing Student Academic Resilience Inventory (NSARI): A mixed-method study.

PLoS One 2021 15;16(6):e0252473. Epub 2021 Jun 15.

Nursing Care Research Center, Semnan University of Medical Sciences, Semnan, Iran.

Introduction: Resilience has been proposed as a suitable solution to better deal with nursing students in cases of challenges but the complex and multidimensional nature of resilience has made its measurement challenging. This study aimed to develop and validate a new inventory theory-driven labeled Nursing Student Academic Resilience Inventory.

Methods: This study was performed with an exploratory sequential mixed-method design. Read More

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Paradoxical paramagnetic calcifications in the globus pallidus: An ex vivo MR investigation and histological validation study.

NMR Biomed 2021 Jun 15:e4571. Epub 2021 Jun 15.

Department of Anatomy, Catholic Institute for Applied Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea.

MR images based on phase contrast images have gained clinical interest as an in vivo tool for assessing anatomical and histological findings. The globus pallidus is an area of major iron metabolism and storage in the brain tissue. Calcium, another important metal in the body, is frequently deposited in the globus pallidus as well. Read More

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RNA-binding protein Hfq plays a vital role in cellulose decomposition throughout affecting cellulase gene expression.

Biotechnol Lett 2021 Jun 15. Epub 2021 Jun 15.

The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan Province, China.

Objective: To study the function of the RNA-binding protein Hfq in Bacillus subtilis cellulose decomposition.

Results: In the medium with sodium carboxymethylcellulose (Na-CMC) as the sole carbon source, the knockout of Hfq resulted in a 38.0% ± 2. Read More

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The Fluorescent Dye 1,6-Diphenyl-1,3,5-hexatriene Binds to Amyloid Fibrils Formed by Human Amylin and Provides a New Probe of Amylin Amyloid Kinetics.

Biochemistry 2021 Jun 15. Epub 2021 Jun 15.

Graduate Program in Biochemistry and Structural Biology, Stony Brook University, Stony Brook, New York 11794, United States.

The fluorescent dye 1,6-diphenyl-1,3,5-hexatriene (DPH) is widely used as a probe of membrane order. We show that DPH also interacts with amyloid fibrils formed by human amylin (h-amylin, also known as islet amyloid polypeptide) in solution, and this results in a 100-fold increase in DPH fluorescence for a sample of 20 μM h-amylin and 0.25 μM DPH. Read More

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Overcoming stochastic variations in culture variables to quantify and compare growth curve data.

Bioessays 2021 Jun 14:e2100108. Epub 2021 Jun 14.

Molecular & Cellular Biochemistry Department, Indiana University, Bloomington, Indiana, USA.

The comparison of growth, whether it is between different strains or under different growth conditions, is a classic microbiological technique that can provide genetic, epigenetic, cell biological, and chemical biological information depending on how the assay is used. When employing solid growth media, this technique is limited by being largely qualitative and low throughput. Collecting data in the form of growth curves, especially automated data collection in multi-well plates, circumvents these issues. Read More

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Accuracy comparison of various quantitative [Tc]Tc-DPD SPECT/CT reconstruction techniques in patients with symptomatic hip and knee joint prostheses.

EJNMMI Res 2021 Jun 14;11(1):60. Epub 2021 Jun 14.

Division of Nuclear Medicine, University Hospital Basel, Basel, Switzerland.

Background: There is a need for better diagnostic tools that identify loose total hip and knee arthroplasties. Here, we present the accuracy of different Tc-dicarboxypropandiphosphate ([Tc]Tc-DPD) SPECT/CT quantification tools for the detection of loose prostheses in patients with painful hip and knee arthroplasties.

Methods: Quantitative reconstruction of mineral phase SPECT data was performed using Siemens xSPECT-Quant and xSPECT-Bone, with and without metal artefact reduction (iMAR) of CT-data. Read More

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CircASXL1 knockdown represses the progression of colorectal cancer by downregulating GRIK3 expression by sponging miR-1205.

World J Surg Oncol 2021 Jun 14;19(1):176. Epub 2021 Jun 14.

Department of General Surgery, Shanghai Fengxian Central Hospital, No. 6600, Nanfeng Road, Nanqiao New Town, Fengxian District, Shanghai, 201400, China.

Background: Colorectal cancer (CRC) is a common aggressive tumor that poses a heavy burden to human health. An increasing number of studies have reported that circular RNA (circRNA) is involved in the progression of CRC. In this study, the special profiles of circASXL1 (circ_0001136) in CRC progression were revealed. Read More

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A novel external beam radiotherapy method for cervical cancer patients using virtual straight or bending boost areas; an in-silico feasibility study.

Radiat Oncol 2021 Jun 14;16(1):110. Epub 2021 Jun 14.

Varian Medical Systems, Palo Alto, USA.

Aim: To investigate the potential role of a novel spatially fractionated radiation therapy (SFRT) method where heterogeneous dose patterns are created in target areas with virtual rods, straight or curving, of variable position, diameter, separation and alignment personalised to a patient's anatomy. The images chosen for this study were CT scans acquired for the external beam part of radiotherapy.

Methods: Ten patients with locally advanced cervical cancer were retrospectively investigated with SFRT. Read More

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Biodegradation of an essential oil UVCB - Whole substance testing and constituent specific analytics yield biodegradation kinetics of mixture constituents.

Chemosphere 2021 Sep 1;278:130409. Epub 2021 Apr 1.

Technical University of Denmark, Department of Environmental Engineering, Building 115, 2800 Kgs, Lyngby, Denmark. Electronic address:

Testing and assessing the persistency, bioaccumulative and toxic properties of UVCBs (substances of Unknown or Variable composition, Complex reaction products or Biological materials) pose major technical and analytical challenges. The main aim of this study was to combine whole substance biodegradation testing with constituent specific analytics for determining primary biodegradation kinetics of the main UVCB constituents. An additional aim was to link the primary biodegradation kinetics of the main constituents to the bioaccumulation potential and baseline toxicity potential of the UVCB. Read More

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September 2021

A side-chain engineering strategy for constructing fluorescent dyes with direct and ultrafast self-delivery to living cells.

Chem Sci 2019 Dec 4;11(3):661-670. Epub 2019 Dec 4.

Center of Bio & Micro/Nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University Jinan 250100 P. R. China

Organic fluorescent dyes with excellent self-delivery to living cells are always difficult to find due to the limitation of the plasma membrane having rigorous selectivity. Herein, in order to improve the permeability of dyes, we utilize a side-chain engineering strategy (SCES): adjusting the side-chain length of dyes to fine-tune the adsorption and desorption processes on the membrane-aqueous phase interfaces of the outer and inner leaflets of the plasma membrane. For this, a family of fluorescent derivatives () was prepared by functionalizing a styryl-pyridinium fluorophore with alkyl side-chains containing a different carbon number from 1 to 22. Read More

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December 2019

Propensity-score-based meta-analytic predictive prior for incorporating real-world and historical data.

Stat Med 2021 Jun 14. Epub 2021 Jun 14.

Statistical and Quantitative Sciences, Takeda Pharmaceuticals, Cambridge, Massachusetts, USA.

As the availability of real-world data sources (eg, EHRs, claims data, registries) and historical data has rapidly surged in recent years, there is an increasing interest and need from investigators and health authorities to leverage all available information to reduce patient burden and accelerate both drug development and regulatory decision making. Bayesian meta-analytic approaches are a popular historical borrowing method that has been developed to leverage such data using robust hierarchical models. The model structure accounts for various degrees of between-trial heterogeneity, resulting in adaptively discounting the external information in the case of data conflict. Read More

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Significant difference in response of malignant tumor cells of individual patients to photodynamic treatment as revealed by digital holographic microscopy.

J Photochem Photobiol B 2021 Jun 3;221:112235. Epub 2021 Jun 3.

Ioffe Institute, Russian Academy of Sciences, St. Petersburg, Russia.

The investigation of in-vitro response of cell cultures derived from tumor material of individual patients with similar tumor localizations to photodynamic treatment is presented. Tumor types included in the research were renal cell carcinoma, melanoma and alveolar, synovial, lypo- and osteo- sarcomas. Long-term observations of treatment-induced morphological changes in cells were performed by means of digital holographic microscopy. Read More

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Proteomic analysis of pikeperch seminal plasma provides novel insight into the testicular development of domesticated fish stocks.

Animal 2021 Jun 11;15(7):100279. Epub 2021 Jun 11.

Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.

Control of the reproduction of domesticated stocks is considered a prerequisite for aquaculture development of pikeperch. However, knowledge about the physiology of the captive pikeperch male reproductive system and the biology of semen is very limited, especially regarding protein characteristics. The aims of our study were to characterize pikeperch sperm quantity and quality parameters and to analyze changes in the proteome of the same males spawned for the first and second times. Read More

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Population Cellular Kinetics of Lisocabtagene Maraleucel, an Autologous CD19-Directed Chimeric Antigen Receptor T-Cell Product, in Patients with Relapsed/Refractory Large B-Cell Lymphoma.

Clin Pharmacokinet 2021 Jun 14. Epub 2021 Jun 14.

Bristol Myers Squibb, Seattle, WA, USA.

Background And Objectives: Lisocabtagene maraleucel (liso-cel) is a CD19-directed, defined composition, 4-1BB chimeric antigen receptor (CAR) T-cell product administered at equal target doses of CD8 and CD4 CAR T cells. Large between-subject variability has been noted with CAR T-cell therapies; patient characteristics might contribute to CAR T-cell expansion variability. We developed a population cellular kinetic model to characterize the kinetics of the liso-cel transgene, via quantitative polymerase chain reaction assessment after intravenous infusion of liso-cel, and to understand covariates that might influence liso-cel kinetics in individual patients. Read More

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Proteomic Analysis Reveals That Metformin Suppresses PSMD2, STIP1, and CAP1 for Preventing Gastric Cancer AGS Cell Proliferation and Migration.

ACS Omega 2021 Jun 26;6(22):14208-14219. Epub 2021 May 26.

Genome and Systems Biology Degree Program, Academia Sinica and National Taiwan University, Taipei 10617, Taiwan.

Metformin is one of the most widely used anti-diabetic drugs in type-II diabetes treatment. The mechanism of decreasing blood glucose is believed to suppress hepatic gluconeogenesis by increasing muscular glucose uptake and insulin sensitivity. Recent studies suggest that metformin may reduce cancer risk; however, its anticancer mechanism in gastric cancers remains unclear. Read More

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Perspectives and Emotional Experiences of Patients With Chronic Myeloid Leukemia During ENESTPath Clinical Trial and Treatment-Free Remission: Rationale and Protocol of the Italian Substudy.

Front Oncol 2021 26;11:638689. Epub 2021 May 26.

Department of Health Sciences, University of Milan, Milan, Italy.

Achievement of deep molecular response following treatment with a tyrosine kinase inhibitor (TKI) allows for treatment-free remission (TFR) in many patients with chronic myeloid leukemia (CML). Successful TFR is defined as the achievement of a sustained molecular response after cessation of ongoing TKI therapy. The phase 3 ENESTPath study was designed to determine the required optimal duration of consolidation treatment with the second-generation TKI, nilotinib 300 mg twice-daily, to remain in successful TFR without relapse after entering TFR for 12 months. Read More

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The double life of conductive nanopipette: a nanopore and an electrochemical nanosensor.

Chem Sci 2020 Aug 5;11(34):9056-9066. Epub 2020 Aug 5.

Department of Chemistry and Biochemistry, Queens College-CUNY Flushing NY 11367 USA

The continuing interest in nanoscale research has spurred the development of nanosensors for liquid phase measurements. These include nanopore-based sensors typically employed for detecting nanoscale objects, such as nanoparticles, vesicles and biomolecules, and electrochemical nanosensors suitable for identification and quantitative analysis of redox active molecules. In this Perspective, we discuss conductive nanopipettes (CNP) that can combine the advantages of single entity sensitivity of nanopore detection with high selectivity and capacity for quantitative analysis offered by electrochemical sensors. Read More

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si-MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR-145-5p/HMGA2 pathway.

Sheng Tan Jili Chen

Oncol Lett 2021 Aug 3;22(2):585. Epub 2021 Jun 3.

Department of Ophthalmology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China.

Metastasis-associated-lung-adenocarcinoma-transcript-1 (MALAT1) is a long non-coding RNA that is considered a potential tumor marker. The present study aimed to investigate the effect and mechanism of MALAT1 on cell proliferation and apoptosis in thymic cancer cells. IU-TAB-1, A549, HCT-116 and 293T cells were screened by reverse transcription-quantitative PCR to assess high-mobility group AT-hook 2 (HMGA2) expression in various types of cancer cells and were transfected with small interfering (si)RNA targeting MALAT1 (si-MALAT1). Read More

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miR-497 inhibits proliferation and invasion in triple-negative breast cancer cells via YAP1.

Oncol Lett 2021 Aug 2;22(2):580. Epub 2021 Jun 2.

Department of Breast and Thyroid Surgery, Shanghai No. 10 People's Hospital, Clinical College of Nanjing Medical University, Shanghai 200072, P.R. China.

MicroRNA (miR)-497 has been reported as a tumor suppressor in various cancer types. Nonetheless, the regulation of triple-negative breast cancer (TNBC) by miR-497 remains poorly understood. The present study aimed to investigate the potential function and mechanism of miR-497 in TNBC. Read More

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MEDI6012: Recombinant Human Lecithin Cholesterol Acyltransferase, High-Density Lipoprotein, and Low-Density Lipoprotein Receptor-Mediated Reverse Cholesterol Transport.

J Am Heart Assoc 2021 Jun 14:e014572. Epub 2021 Jun 14.

Early Clinical Development Research and Early Development Cardiovascular, Renal and Metabolism BioPharmaceuticals R&D AstraZeneca Gaithersburg MD.

Background MEDI6012 is recombinant human lecithin cholesterol acyltransferase, the rate-limiting enzyme in reverse cholesterol transport. Infusions of lecithin cholesterol acyltransferase have the potential to enhance reverse cholesterol transport and benefit patients with coronary heart disease. The purpose of this study was to test the safety, pharmacokinetic, and pharmacodynamic profile of MEDI6012. Read More

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N-acetylcysteine, xCT and suppression of Maxi-chloride channel activity in human placenta.

Placenta 2021 Jun 5;110:46-55. Epub 2021 Jun 5.

Faculty of Medicine, University of Southampton, UK; Institute for Life Sciences, University of Southampton, UK.

Introduction: Placental oxidative stress features in pregnancy pathologies but in clinical trials antioxidant supplementation has not improved outcomes. N-acetylcysteine (NAC) stimulates glutathione production and is proposed as a therapeutic agent in pregnancy. However, key elements of N-acetylcysteine biology, including its cellular uptake mechanism, remains unclear. Read More

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Predicting the relationship between PFAS component signatures in water and non-water phases through mathematical transformation: Application to machine learning classification.

Chemosphere 2021 Jun 5;282:131097. Epub 2021 Jun 5.

School of Civil and Environmental Engineering UNSW Sydney Manly Vale, NSW, 2093, Australia.

Per- and polyfluoroalkyl substances (PFAS) are widespread in the environment, as a result of decades of use across a range of applications. While PFAS contamination often enters the environment in the aqueous phase, PFAS is regularly detected in a range of different phases, including soils, sediments and biota. Although PFAS at a given site may originate from the same sources, the compositions observed in different phases are nearly always different, a fact that can complicate source allocation efforts. Read More

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Quantitative analysis and spatial and temporal distribution of volatile organic compounds in atmospheric air by utilizing drone with miniaturized samplers.

Chemosphere 2021 Jun 7;282:131024. Epub 2021 Jun 7.

Department of Chemistry and Institute for Atmospheric and Earth System Research, P.O. Box 55, FI-00014, University of Helsinki, Finland. Electronic address:

Our second generation air sampling drone system, allowing the simultaneous use of four solid phase microextraction (SPME) Arrow and four in-tube extraction (ITEX) units, was employed for collection of atmospheric air samples at different spatial and temporal dimensions. SPME Arrow coated with two types of materials and ITEX with 10% polyacrylonitrile as sorbent were used to give a more comprehensive chemical characterization of the collected air samples. Before field sampling, miniaturized samplers went through quality control and assurance in terms of reproducibility (RSD ≤14. Read More

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Validation of "Care of the Dying Evaluation" in Emergency Medicine (CODE-EM): pilot phase of end-of-life management protocol offered within emergency room (EMPOWER) study.

Ann Palliat Med 2021 May 25. Epub 2021 May 25.

Emergency Medicine Department, National University Hospital, National University Health System, Singapore; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Background: An increasing number of patients who present to emergency departments are at their endof-life phase and have significant palliative care needs such as in symptom control for pain and dyspnoea. Evaluating quality of care provided is imperative, yet there is no suitable tool validated in the emergency and Asian settings. We aim to examine the face and construct validity, and reliability of a newly developed questionnaire, Care of the Dying Evaluation - Emergency Medicine, for measuring the quality of end-of-life care in an Asian emergency context. Read More

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MiR-133a-3p inhibits the malignant progression of esophageal cancer by targeting CDCA8.

J Biochem 2021 Jun 12. Epub 2021 Jun 12.

Department of Thoracic Surgery, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou City, Fujian Province, 350001, China.

Objective: To explore the interaction between miR-133a-3p and CDCA8 in esophageal cancer (EC) and their effect on malignant behavior of EC cells.

Methods: Differential miRNAs and mRNAs were obtained from The Cancer Genome Atlas (TCGA) database. Quantitative real-time PCR (qRT-PCR) was used to detect the expression levels of miR-133a-3p and CDCA8 mRNA in EC cells. Read More

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Observation of varicella zoster virus-induced acute retinal necrosis: viral load detection and visual outcome.

Eye (Lond) 2021 Jun 11. Epub 2021 Jun 11.

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.

Objectives: To observe the changes of viral load in aqueous humour samples and visual outcomes in varicella zoster virus (VZV)-induced acute retinal necrosis (ARN).

Methods: Observational retrospective study. Medical records and viral load measured by real-time quantitative polymerase chain reaction (qPCR) of 20 eyes with VZV-induced ARN were reviewed. Read More

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Downregulation of decidual SKP2 is associated with human recurrent miscarriage.

Reprod Biol Endocrinol 2021 Jun 11;19(1):88. Epub 2021 Jun 11.

Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200135, China.

Background: Recurrent miscarriage (RM) is a very frustrating problem for both couples and clinicians. To date, the etiology of RM remains poorly understood. Decidualization plays a critical role in implantation and the maintenance of pregnancy, and its deficiency is closely correlated with RM. Read More

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