209,148 results match your criteria quantitative pcr


Structure and cytotoxicity of trichothecenes produced by the potato-associated fungus Trichothecium crotocinigenum.

Bioorg Chem 2021 Apr 5;111:104874. Epub 2021 Apr 5.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, People's Republic of China; The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, People's Republic of China. Electronic address:

Seven previously undescribed trichothecenes, named trichothecrotocins M-S (1-7), along with five known compounds, were isolated from rice cultures of the potato-associated fungus Trichothecium crotocinigenum. Their structures and absolute configurations were determined through spectroscopic methods, single-crystal X-ray diffraction, and quantum chemistry calculations on ECD. Compound 1 possesses a rare 6,11-epoxy moiety in the trichothecene family. Read More

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SLC6A3 as a potential circulating biomarker for gastric cancer detection and progression monitoring.

Pathol Res Pract 2021 Apr 14;221:153446. Epub 2021 Apr 14.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China. Electronic address:

Background: Gastric cancer is a malignant tumor originating from the gastric mucosal epithelium, with no obvious symptoms at the early stage. The dopamine transporter gene (SLC6A3) is involved in the metabolism of dopamine and catecholamine and is a potential gene for Parkinson's disease and alcoholism. But the role of SLC6A3 in gastric cancer is still unknown. Read More

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Chemerin causes lipid metabolic imbalance and induces passive lipid accumulation in human hepatoma cell line via the receptor GPR1.

Life Sci 2021 Apr 19:119530. Epub 2021 Apr 19.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology & College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:

Aims: Chemerin is abundant in patients with high body mass index and metabolic syndrome possibly due to its activation in adipogenesis and glucose intolerance. It has reported that sera chemerin is positively associated with fatty liver with little known underlying mechanisms. Our aim is to study the role of chemerin in hepatic lipid metabolism. Read More

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Establishing a gold standard method for the detection of Cherax reovirus using reverse transcriptase, quantitative, polymerase chain reaction.

J Virol Methods 2021 Apr 19:114169. Epub 2021 Apr 19.

College of Public Health, Medical and Veterinary Sciences, James Cook University, 1 Solander Road, Townsville, QLD, 4811, Australia. Electronic address:

Cherax reovirus infects redclaw crayfish (Cherax quadricarinatus) and it may be involved in mortalities between 5-20% and stunting of up to 40% of survivors. The sequence of the RNA-dependent RNA polymerase was used to develop a reverse transcription, quantitative, PCR (RT-qPCR) which was specific against seven other crustacean viruses (Athtab bunyavirus, Chequa iflavirus, Macrobrachium rosenbergii nodavirus, Gill-associated virus, Taura syndrome virus, White spot syndrome virus, and Penaeus stylirostris Penstylhamaparvovirus) although GAV produced a reaction that was easily separated by melt curve analysis. A strong linear correlation (r = 0. Read More

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PcG Proteins MSI1 and BMI1 Function Upstream of miR156 to Regulate Aerial Tuber Formation in Potato1[OPEN].

Plant Physiol 2020 Jan;182(1):185-203

Biology Division, Indian Institute of Science Education and Research, Pune 411008, Maharashtra, India.

Polycomb Repressive Complexes (PRC1 and PRC2) regulate developmental transitions in plants. AtBMI1, a PRC1 member, represses micro RNA156 (miR156) to trigger the onset of adult phase in Arabidopsis (Arabidopsis thaliana). miR156 overexpression (OE) reduces below-ground tuber yield, but stimulates aerial tubers in potato (Solanum tuberosum ssp andigena) under short-day (SD) photoperiodic conditions. Read More

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January 2020

Octreotide inhibits secretion of IGF-1 from orbital fibroblasts in patients with thyroid-associated ophthalmopathy via inhibition of the NF-κB pathway.

PLoS One 2021 22;16(4):e0249988. Epub 2021 Apr 22.

Department of Ophthalmology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Purpose: We investigated the effect of octreotide, a long-acting somatostatin (SST) analogue, on IGF-1 secretion and its possible mechanism of action in orbital fibroblasts (OFs) from patients with thyroid-associated ophthalmopathy (TAO).

Materials And Methods: OFs were isolated from the orbital fat of patients with TAO or healthy individuals. The expression level of insulin-like growth factor (IGF)-1, at the protein and mRNA level, was determined with ELISA and quantitative RT-PCR, respectively. Read More

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Prevalence, clinical features, and outcomes of SARS-CoV-2 infection in pregnant women with or without mild/moderate symptoms: Results from universal screening in a tertiary care center in Mexico City, Mexico.

PLoS One 2021 22;16(4):e0249584. Epub 2021 Apr 22.

Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.

The perinatal consequences of SARS-CoV-2 infection are still largely unknown. This study aimed to describe the features and outcomes of pregnant women with or without SARS-CoV-2 infection after the universal screening was established in a large tertiary care center admitting only obstetric related conditions without severe COVID-19 in Mexico City. This retrospective case-control study integrates data between April 22 and May 25, 2020, during active community transmission in Mexico, with one of the highest COVID-19 test positivity percentages worldwide. Read More

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Surveillance of densoviruses and mesomycetozoans inhabiting grossly normal tissues of three Aotearoa New Zealand asteroid species.

PLoS One 2021 22;16(4):e0241026. Epub 2021 Apr 22.

School of Biological Sciences, University of Auckland, Auckland, New Zealand.

Asteroid wasting events and mass mortality have occurred for over a century. We currently lack a fundamental understanding of the microbial ecology of asteroid disease, with disease investigations hindered by sparse information about the microorganisms associated with grossly normal specimens. We surveilled viruses and protists associated with grossly normal specimens of three asteroid species (Patiriella regularis, Stichaster australis, Coscinasterias muricata) on the North Island / Te Ika-a-Māui, Aotearoa New Zealand, using metagenomes prepared from virus and ribosome-sized material. Read More

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Resistin induces chemokine and matrix metalloproteinase production via CAP1 receptor and activation of p38-MAPK and NF-κB signalling pathways in human chondrocytes.

Clin Exp Rheumatol 2021 Apr 14. Epub 2021 Apr 14.

Department of Orthopaedic Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.

Objectives: Adipokine resistin is highly expressed in the serum and synovial uid (SF) of patients with knee osteoarthritis (KOA) but its pathogenic role in KOA remains unclear. We aimed to explore the mechanism of resistin/CAP1 in human KOA chondrocytes.

Methods: We enrolled 103 patients with radiographic KOA and 86 healthy participants as controls. Read More

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BZW1 promotes cell proliferation in prostate cancer by regulating TGF-β1/Smad pathway.

Cell Cycle 2021 Apr 22:1-9. Epub 2021 Apr 22.

Department of Urology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Recently, basic leucine zipper and the W2 domain-containing protein 1 (BZW1) are reported to be implicated in tumor progression. However, the role of BZW1 in prostate cancer remains unknown. This study is aimed to investigate the expression of BZW1 and its influence on cell proliferation in prostate cancer. Read More

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Quantification of P-Glycoprotein in the Gastrointestinal Tract of Humans and Rodents: Methodology, Gut Region, Sex, and Species Matter.

Mol Pharm 2021 Apr 22. Epub 2021 Apr 22.

UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, U.K.

Intestinal efflux transporters affect the gastrointestinal processing of many drugs but further data on their intestinal expression levels are required. Relative mRNA expression and relative and absolute protein expression data of transporters are commonly measured by real-time polymerase chain reaction (RT-PCR), Western blot and mass spectrometry-based targeted proteomics techniques. All of these methods, however, have their own strengths and limitations, and therefore, validation for optimized quantification methods is needed. Read More

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LINC00943 knockdown exerts neuroprotective effects in Parkinson's disease through regulates CXCL12 expression by sponging miR-7-5p.

Genes Genomics 2021 Apr 22. Epub 2021 Apr 22.

Department of Neurology, Jingmen No. 1 People's Hospital, No. 168, Xiangshan Avenue, Duodao District, Jingmen, 448000, Hubei, China.

Background: Parkinson's disease (PD) is a common neurodegenerative movement disorder, but the pathogenesis is still unclear. Long non-coding RNAs (lncRNAs) have been reported to play a prominent role in PD.

Objective: This study is designed to explore the role and mechanism of long intergenic non-coding RNA 00943 (LINC00943) in the N-methyl-4-phenylpyridine (MPP)-inducted PD model. Read More

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Polymorphism of TUSC7 associated with gastric cancer susceptibility and binding with miR-133a-3p: a population-based case-control study.

Int J Clin Oncol 2021 Apr 22. Epub 2021 Apr 22.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, 450001, Henan, China.

Background: Previous studies have demonstrated that gene polymorphism is associated with cancer susceptibility. Most of these genes are involved in neoplastic processes. Previous studies demonstrated that tumor-suppressor candidate 7 (TUSC7) was a tumor-suppressor gene in various tumors. Read More

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MicroRNA-25 Exerts an Oncogenic Function by Regulating the Ubiquitin Ligase Fbxw7 in Hepatocellular Carcinoma.

Ann Surg Oncol 2021 Apr 22. Epub 2021 Apr 22.

Department of Gastroenterological Surgery, Graduate School of Life Sciences, Kumamoto University, Kumamoto City, Kumamoto, Japan.

Background: MicroRNA (miRNA) expression abnormalities are implicated in tumor progression. Previous reports have indicated that microRNA-25 (miR-25) acts as a tumor suppressor or oncogene in diverse cancers. However, its molecular mechanisms in hepatocellular carcinoma (HCC) are still unclear. Read More

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Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced injury in human tubule epithelial cells by regulating miR-93-5p/TXNIP axis.

Med Microbiol Immunol 2021 Apr 22. Epub 2021 Apr 22.

Department of Respiratory and Critical Care Medicine, The Fourth People's Hospital of Shaanxi Province, No. 512, Xianning East Road, Xincheng District, Xi'an City, 710043, Shanxi Province, China.

Many long non-coding RNAs (lncRNAs) have been found to play crucial roles in sepsis-induced acute kidney injury (AKI), including lncRNA nuclear-enriched abundant transcript 1 (NEAT1). We aimed to further elucidate the functions and molecular mechanism of NEAT1 in sepsis-induced AKI. Sepsis-induced AKI cell model was established by treatment with lipopolysaccharide (LPS) in human tubule epithelial (HK2) cells. Read More

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The Soybean Sugar Transporter GmSWEET15 Mediates Sucrose Export from Endosperm to Early Embryo.

Plant Physiol 2019 Jul;180(4):2133-2141

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, People's Republic of China.

Soybean (Glycine max) seed is primarily composed of a mature embryo that provides a major source of protein and oil for humans and other animals. Early in development, the tiny embryos grow rapidly and acquire large quantities of sugars from the liquid endosperm of developing seeds. An insufficient supply of nutrients from the endosperm to the embryo results in severe seed abortion and yield reduction. Read More

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Maize Dek44 Encodes Mitochondrial Ribosomal Protein L9 and Is Required for Seed Development.

Plant Physiol 2019 Jul;180(4):2106-2119

Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China.

Mitochondrial respiration depends on proteins encoded by the nuclear and mitochondrial genomes. Many respiratory chain-related proteins are encoded by the mitochondrial genome and undergo translation by mitochondrial ribosomes. The newly identified maize (Zea mays) defective kernel44 (dek44) mutant produces small kernels showing embryo-lethal phenotypes. Read More

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Maternal and Neonatal Morbidity and Mortality Among Pregnant Women With and Without COVID-19 Infection: The INTERCOVID Multinational Cohort Study.

JAMA Pediatr 2021 Apr 22. Epub 2021 Apr 22.

Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, United Kingdom.

Importance: Detailed information about the association of COVID-19 with outcomes in pregnant individuals compared with not-infected pregnant individuals is much needed.

Objective: To evaluate the risks associated with COVID-19 in pregnancy on maternal and neonatal outcomes compared with not-infected, concomitant pregnant individuals.

Design, Setting, And Participants: In this cohort study that took place from March to October 2020, involving 43 institutions in 18 countries, 2 unmatched, consecutive, not-infected women were concomitantly enrolled immediately after each infected woman was identified, at any stage of pregnancy or delivery, and at the same level of care to minimize bias. Read More

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miR-498/DNMT3b Axis Mediates Resistance to Radiotherapy in Esophageal Cancer Cells.

Cancer Biother Radiopharm 2021 Apr 20. Epub 2021 Apr 20.

Department of Gastroenterology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, P.R. China.

To explore the role of miR-498 in the radiotherapy resistance of esophageal cancer (EC) and its underlying mechanism. models of EC tissues with radioresistance or radiosensitivity were isolated from 72 EC patients who received radiotherapy. models were established after irradiation of KYSE30 cells. Read More

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Glucose deprivation affects the expression of genes encoding cAMP-activated protein kinase and related proteins in U87 glioma cells in ERN1 dependent manner.

Endocr Regul 2020 Nov 24;54(4):244-254. Epub 2020 Nov 24.

Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine.

The aim of this investigation was to study the expression of genes encoding cAMP-activated protein kinase catalytic and regulatory A subunits (PRKACA and PRKAR1A) and related proteins such as cAMP-dependent protein kinase inhibitors A and G (PKIA and PKIG), catalytic subunit A of protein phosphatase 3 (PPP3CA), A-kinase anchoring protein 12 (AKAP12), and praja ring finger ubiquitin ligase 2 (PJA2) in U87 glioma cells in response to glucose deprivation in both control U87 glioma cells and cells with ERN1 (endoplasmic reticulum to nucleus signaling 1) knockdown, the major pathway of the endoplasmic reticulum stress signaling, for evaluation of possible significance of glucose deprivation in ERN1 dependent regulation of glioma growth. The expression level of PRKA related genes was studied in control (transfected by vector) and ERN1 knockdown U87 glioma cells under glucose deprivation by real-time quantitative polymerase chain reaction. It was shown that the expression level of and genes was down-regulated in control glioma cells treated by glucose deprivation, but gene was up-regulated. Read More

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November 2020

Detection of RGNNV genotype betanodavirus in the Black Sea and monitoring studies.

Dis Aquat Organ 2021 Apr 22;144:117-121. Epub 2021 Apr 22.

Izmir/Bornova Veterinary Control Institute, Virology Department, 35010, Izmir, Turkey.

Viral nervous necrosis (VNN), caused by betanodavirus, is a significant viral infection that threatens marine aquaculture. Freshwater and marine fish farms in Turkey are subjected to annual pathogen screenings. In 2016, during the Nervous Necrosis Virus screening program conducted in the Black Sea, betanodavirus was unexpectedly detected using real-time reverse transcription-polymerase chain reaction in apparently healthy sea bass. Read More

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Expression of Tmem41b and MMP13 associated with poor outcome in osteosarcomas.

Neoplasma 2021 Apr 23. Epub 2021 Apr 23.

Department of Surgical Medicine, Hebei Medical University, Shijiazhuang, Hebei, China.

Osteosarcoma (OS) is a malignant bone sarcoma characterized by a propensity for metastatic spread. Tmem41b is a multi-spanning membrane protein that acts as a novel autophagy-related (ATG) gene; however, its effect on the malignant phenotypes of tumor cells and the corresponding molecular details remains unknown. In the current study, RNA-sequencing, quantitative PCR (qPCR), and immunohistochemical analysis were conducted to prove Tmem41b upregulation in 103 OS tissue specimens and three OS cell lines (U-2OS, U87, and MG63). Read More

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[Effects of Activated Carbon on the Fate of Antibiotic Resistance Genes During Anaerobic Digestion of the Organic Fraction of Municipal Solid Waste].

Huan Jing Ke Xue 2021 May;42(5):2413-2421

Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.

The organic fraction of municipal solid waste (OFMSW) has become one of the sources and reservoirs of antibiotic resistance genes (ARGs). It is essential to explore the fate of ARGs during biological treatment of OFMSW. Therefore, the changes in several types of ARGs and integron genes during anaerobic digestion of the OFMSW were analyzed by quantitative PCR. Read More

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[High-Throughput Sequencing Analysis of Microbial Communities in Summertime Atmospheric Particulate Matter in Hefei City].

Huan Jing Ke Xue 2021 May;42(5):2169-2178

Institute of Polar Environment & Anhui Key Laboratory of Polar Environment and Global Change, School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China.

The composition, physical and chemical properties, sources, and temporal and spatial changes in airborne particulate matter have been extensively investigated in previous studies. However, less is known about bioaerosols, which are mainly composed of bacteria and fungi and constitute up to 25% of the total airborne particulate matter. In this study, we used inductively coupled plasma mass spectrometry and ion chromatography to determine the concentrations of trace elements and water-soluble ions in atmospheric particulates, respectively. Read More

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Expression profiles of genes involved in fatty acid and lipid biosynthesis in developing seeds of Paeonia ostii.

Genes Genomics 2021 Apr 21. Epub 2021 Apr 21.

Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou, 466001, Henan, China.

Background: Paeonia ostii seeds were identified as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefits. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds.

Objectives: This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development. Read More

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Correlation between the immune checkpoints and EMT genes proposes potential prognostic and therapeutic targets in ESCC.

J Mol Histol 2021 Apr 21. Epub 2021 Apr 21.

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

PD-1, PD-L1, CTLA-4, TIM-3, and LAG-3, crucial immune checkpoint molecules in the tumor microenvironment, identify as key targets for cancer immunotherapy. There is a correlation between immune cells and epithelial-mesenchymal transition (EMT)-related genes expression in varies human cancers. In this study, we aimed to investigate the probable association between expression of immune checkpoints and EMT in esophageal squamous cell carcinoma (ESCC) with clinical treats for providing the new therapeutic targets and prognostic value for the disease. Read More

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Is Point-of-Care testing feasible and safe in care homes in England? An exploratory usability and accuracy evaluation of a Point-of-Care Polymerase Chain Reaction test for SARS-COV-2.

Age Ageing 2021 Apr 21. Epub 2021 Apr 21.

NIHR London In Vitro Diagnostics Co-operative, Dept of Surgery and Cancer, Faculty of Medicine, Imperial College London St. Mary's Hospital London.

Introduction: Reliable rapid testing for COVID-19 is needed in care homes to reduce the risk of outbreaks and enable timely care. This study aimed to examine the usability and test performance of a point of care polymerase chain reaction (PCR) test for detection of SARS-COV2 (POCKITTM Central) in care homes.

Methods: POCKITTM Central was evaluated in a purposeful sample of four UK care homes. Read More

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COMPARATIVE ANALYSIS OF qPCR MEASUREMENT OF HIV VIRAL LOAD AND ELISA DETECTION OF p24 ANTIGEN AFTER HYPERBARIC OXYGEN EXPOSURE.

Afr J Infect Dis 2020 31;14(2):53-59. Epub 2020 Jul 31.

Department of HIV, Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia.

Background: A decrease in the number of viruses or viral nucleic acid components will determine whether a therapy successfully eradicates the virus. Sensitivity and specificity are needed to enable easy, precise and efficient diagnosis and evaluation of therapy. This study examined the sensitivity of quantitative PCR (qPCR) for detecting viral nucleic acids as compared with enzyme-linked immunosorbent assay (ELISA) for detecting the human immunodeficiency virus 1 (HIV-1) p24 antigen after hyperbaric oxygen therapy. Read More

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Circular DNA enrichment sequencing reveals the viral/satellites genetic diversity associated with the third epidemic of cotton leaf curl disease.

Biol Methods Protoc 2021 25;6(1):bpab005. Epub 2021 Mar 25.

Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad 38000, Pakistan.

Cotton leaf curl disease (CLCuD) is the most important limiting factor for cotton production in Pakistan. The CLCuD passed through two major epidemics in this region with distinct begomoviruses/satellites complexes. Since 2015 the disease has again started to appear in epidemic form, causing heavy losses to cotton crop, which we termed as the "third epidemic". Read More

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Facilitating Test Status Information Communication in the Time of COVID-19.

Acad Pathol 2021 Jan-Dec;8:23742895211006822. Epub 2021 Apr 9.

Department of Pathology, Children's Hospital of Philadelphia, PA, USA.

The Coronavirus 2019 pandemic has strained nearly every aspect of pathology practice, including preanalytic, analytic, and postanalytic processes. Much of the challenges result from high demand for limited severe acute respiratory syndrome coronavirus 2 testing capacity, a resource required to facilitate patient flow throughout the hospital system and society at large. At our institution, this led to unprecedented increases in inquiries from providers to laboratory staff relating to the expected time to result for their patients. Read More

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