J Virol Methods 2021 Apr 19:114169. Epub 2021 Apr 19.
College of Public Health, Medical and Veterinary Sciences, James Cook University, 1 Solander Road, Townsville, QLD, 4811, Australia. Electronic address:
Cherax reovirus infects redclaw crayﬁsh (Cherax quadricarinatus) and it may be involved in mortalities between 5-20% and stunting of up to 40% of survivors. The sequence of the RNA-dependent RNA polymerase was used to develop a reverse transcription, quantitative, PCR (RT-qPCR) which was speciﬁc against seven other crustacean viruses (Athtab bunyavirus, Chequa iflavirus, Macrobrachium rosenbergii nodavirus, Gill-associated virus, Taura syndrome virus, White spot syndrome virus, and Penaeus stylirostris Penstylhamaparvovirus) although GAV produced a reaction that was easily separated by melt curve analysis. A strong linear correlation (r = 0. Read More