14,160 results match your criteria quantified flow

Quantifying effects of reverse linear perspective as a visual cue on vehicle and platoon crash risk variations in car-following using path analysis.

Accid Anal Prev 2021 Jun 12;159:106215. Epub 2021 Jun 12.

School of Civil Engineering and Architecture, Wuhan Institute of Technology, Wuhan, 430205, China. Electronic address:

Road markings are prevalent in practice as perceptual countermeasures to crashes, and a great deal of them have been used for speed reduction. However, there is rare seen any equivalent measures especially for distance control. More importantly, the visual perceptual mechanism of road markings on driving behaviors and crash risk is still blur. Read More

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Differential Effect of Proinflammatory Cytokines on Corneal and Conjunctival Epithelial Cell Mucins and Glycocalyx.

Transl Vis Sci Technol 2021 Jun;10(7):17

Chapman University School of Pharmacy, Chapman University, Irvine, CA, USA.

Purpose: Ocular surface mucins and glycocalyx are critical for providing ocular hydration as well lubrication and repelling pathogens or allergens. Elevated levels of tear proinflammatory cytokines in dry eye may have detrimental effect on mucins and glycocalyx. The present study tested the effect of proinflammatory cytokines IL-6, TNF-α, and IFN-γ on membrane-tethered mucins expression, glycocalyx, and viability of ocular surface epithelial cells. Read More

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Brain networks in patients with isolated or recurrent transient global amnesia.

Acta Neurol Scand 2021 Jun 15. Epub 2021 Jun 15.

Department of Neurology, Haeundae Paik Hospital, Inje University College of Medicine, Busan, Korea.

Objectives: The aim of this study was to investigate differences in cerebral blood flow (CBF) and functional networks between transient global amnesia (TGA) patients with a single event and those with recurrent events using arterial spin labeling (ASL) MRI.

Methods: We enrolled patients with TGA and classified them into two groups according to the number of TGA events: TGA patients with a single event and those with recurrent events. MRI scans were performed within 24 h after TGA ictal onset in all patients. Read More

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SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells.

Respir Res 2021 Jun 14;22(1):178. Epub 2021 Jun 14.

Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, 200032, China.

Background: Serine peptidase inhibitor, clade B, member 10 (SERPINB10) contributes to allergic inflammation in asthma. However, its role in the T-helper type 2 (Th2) response of allergic asthma is not known. The goal of this study was to unveil the function of SERPINB10 in the Th2 response of allergic asthma and the mechanism by which SERPINB10 affects the viability of Th2 cells. Read More

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Effect of Surface Nanopatterning on Slip: The Case of Couette Flow of Long-Chain Polyethylene Melt Flowing Past Gold Surfaces.

J Phys Chem B 2021 Jun 15. Epub 2021 Jun 15.

School of Chemical Engineering, National Technical University of Athens (NTUA), Athens, GR-15780, Greece.

The manifestation of slip during flow of a polymer melt past a solid surface depends on several parameters, such as film thickness, the strength of polymer-solid interactions compared to the cohesive energy of the polymer, and the roughness of the surface. Understanding the role of these molecular aspects for slip is crucial in microfluidics, friction-tuning, polymer extrusion, and nanocomposites applications. The present article investigates the effect of surface nanopatterning on slip, via Couette-flow simulations of long chain polyethylene melts past nanopatterned gold surfaces. Read More

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Wall teichoic acids govern cationic gold nanoparticle interaction with Gram-positive bacterial cell walls.

Chem Sci 2020 Mar 23;11(16):4106-4118. Epub 2020 Mar 23.

Department of Chemistry, University of Wisconsin-Madison 1101 University Avenue Madison WI 53706 USA

Molecular-level understanding of nanomaterial interactions with bacterial cell surfaces can facilitate design of antimicrobial and antifouling surfaces and inform assessment of potential consequences of nanomaterial release into the environment. Here, we investigate the interaction of cationic nanoparticles with the main surface components of Gram-positive bacteria: peptidoglycan and teichoic acids. We employed intact cells and isolated cell walls from wild type and two mutant strains differing in wall teichoic acid composition to investigate interaction with gold nanoparticles functionalized with cationic, branched polyethylenimine. Read More

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Transit Time Measurement in Indicator Dilution Curves: Overcoming the Missing Ground Truth and Quantifying the Error.

Front Physiol 2021 28;12:588120. Epub 2021 May 28.

Institute of Biomedical Engineering, Karlsruhe Institute of Technology, Karlsruhe, Germany.

The vascular function of a vessel can be qualitatively and intraoperatively checked by recording the blood dynamics inside the vessel via fluorescence angiography (FA). Although FA is the state of the art in proving the existence of blood flow during interventions such as bypass surgery, it still lacks a quantitative blood flow measurement that could decrease the recurrence rate and postsurgical mortality. Previous approaches show that the measured flow has a significant deviation compared to the gold standard reference (ultrasonic flow meter). Read More

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Application of ICH Guidelines for Studying the Degradation Behavior of Rocuronium Bromide Coupled with Stability-Indicating RP-LC Method.

J Chromatogr Sci 2021 Jun 12. Epub 2021 Jun 12.

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo 11562, Egypt.

Non-depolarizing neuromuscular blocking agent Rocuronium bromide was quantified in drug substance and drug product using reversed-phase liquid chromatographic method. Forced degradation studies were conducted for Rocuronium bromide in drug substance under acidic (2MHCl), basic (2MNaOH), oxidative (3% H2O2), thermal (135°C) and photolytic (254 nm) stress conditions. An Agilent H12 C18 column was used for separation using diammonium hydrogen phosphate buffer (pH 8; 0. Read More

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L-Arginine reduces downstream vascular contractility after flow-diverting device deployment: A preliminary study in a rabbit model.

Interv Neuroradiol 2021 Jun 12:15910199211025107. Epub 2021 Jun 12.

Department of Radiology, Mayo Clinic Rochester, Rochester, MN, USA.

Background: Flow diverters (FDs) are an effective treatment for intracranial aneurysms, though not free from hemorrhagic complications. A previous study demonstrated increased vascular contractility after FD-implantation as a potential mechanism of distal complications. Our study aimed to investigate whether L-arginine medication affects vascular contractility following FD deployment in a rabbit model. Read More

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Immunophenotypic characterization of double positive T lymphocytes in Cuban older adults.

Exp Gerontol 2021 Jun 10:111450. Epub 2021 Jun 10.

Subdirection for Medical Assistance, Longevity, Anging and Health Research Center (CITED), Vedado, Havana, Cuba. Electronic address:

Introduction: Immunosenescence is the functional deterioration of the immune system during natural aging that mainly affects T lymphocytes, within which is the subpopulation of peripheral double positive T lymphocytes that are immunomodulators of the immune response. They are increased in older adults and play a fundamental role in the immunopathogenesis of autoimmune diseases, viral infections and neoplasms.

Objective: To characterize the immunophenotype of peripheral double positive T lymphocytes in Cuban older adults. Read More

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Metabolic maturation of differentiating cardiosphere-derived cells.

Stem Cell Res 2021 Jun 5;54:102422. Epub 2021 Jun 5.

Department of Physiology, Anatomy & Genetics, University of Oxford, UK. Electronic address:

Cardiosphere-derived cells (CDCs) can be expanded in vitro and induced to differentiate along the cardiac lineage. To recapitulate the phenotype of an adult cardiomyocyte, differentiating progenitors need to upregulate mitochondrial glucose and fatty acid oxidation. Here we cultured and differentiated CDCs using protocols aimed to maintain stemness or to promote differentiation, including triggering fatty acid oxidation using an agonist of peroxisome proliferator-activated receptor alpha (PPARα). Read More

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Evaluating CO emissions from continuous flow and batch growth systems under autotrophic mode: Implications for GHG accounting of biological nutrient removal.

J Environ Manage 2021 Jun 8;294:112928. Epub 2021 Jun 8.

Department of Chemistry and Biology, Ryerson University, 350 Victoria St., Toronto, ON, M5B 2K3, Canada; Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address:

The oxidation of ammonia by autotrophic bacteria is a central part of the nitrogen cycle and a fundamental aspect of biological nutrient removal (BNR) during wastewater treatment. Autotrophic ammonia oxidation produces protons and results in net-CO production due to the neutralizing effect of bicarbonate alkalinity. Attention must be paid to the propensity for this produced CO to be transferred to the atmosphere where it can act as a greenhouse gas (GHG). Read More

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Oxidative stress-induced senescence mediates inflammatory and fibrotic phenotypes in fibroblasts from systemic sclerosis patients.

Rheumatology (Oxford) 2021 Jun 11. Epub 2021 Jun 11.

Faculty of Pharmacy, Université de Montréal, Québec, Canada.

Objective: Systemic sclerosis (SSc) is an autoimmune connective tissue disorder characterized by inflammation and fibrosis. Although constitutive activation of fibroblasts is proposed to be responsible for the fibrotic and inflammatory features of the disease, the underlying mechanism remains elusive and, effective therapeutic targets are still lacking. The aim of this study was to evaluate the role of oxidative stress-induced senescence and its contribution to the pro-fibrotic and pro-inflammatory phenotypes of fibroblasts from SSc patients. Read More

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MicroRNA-146b-3p regulates the dysfunction of vascular smooth muscle cells via repressing phosphoinositide-3 kinase catalytic subunit gamma.

Bioengineered 2021 Dec;12(1):2627-2638

Department of Cardiac Surgery, Dalian Municipal Center Hospital, Dalian Liaoning, China.

MicroRNAs are crucial regulators in the phenotype switch of vascular smooth muscle cells (VSMCs). Nonetheless, the role of miR-146b-3p in VSMCs remains unclear. In the present study, platelet-derived growth factor-BB (PDGF-BB) at different concentrations was employed to stimulate VSMCs for different times, to establish the model of VSMC dysfunction. Read More

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December 2021

MiR-155 regulates neutrophil extracellular trap formation and lung injury in abdominal sepsis.

J Leukoc Biol 2021 Jun 11. Epub 2021 Jun 11.

Department of Surgery, Clinical Sciences, Malmö, Skåne University Hospital, Lund University, Malmö, Sweden.

Neutrophil extracellular traps (NETs)-mediated tissue damage is a hallmark in abdominal sepsis. Under certain conditions, microRNAs (miRs) can regulate protein expression and cellular functions. The aim of this study was to investigate the role of miR-155 in sepsis-induced NET formation, lung inflammation, and tissue damage. Read More

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Association Between Aneurysmal Haemodynamics and Device Microstructural Characteristics After Flow-Diversion Treatments With Dual Stents of Different Sizes: A Numerical Study.

Front Physiol 2021 25;12:663668. Epub 2021 May 25.

Biomedical Flow Dynamics Laboratory, Institute of Fluid Science, Tohoku University, Sendai, Japan.

Objectives: Treating intracranial aneurysms with flow-diverting stents sometimes requires deployment of a second device. Herein we quantify the sizing effects of devices in dual-stent treatments upon the final stent microstructure and the post-treatment aneurysmal haemodynamics.

Methods: Fifteen sidewall ICA aneurysm geometries were included. Read More

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Topical NSAIDs for acute local pain relief: characterization of drug delivery profiles into and through human skin.

Drug Dev Ind Pharm 2021 Jun 10:1-30. Epub 2021 Jun 10.

Reckitt Healthcare Ltd, Dansom Lane, Hull, HU8 7DS, UK.

The efficacy of topical nonsteroidal anti-inflammatory drugs (NSAIDs) relates not only to the individual NSAID used but also to differences in formulation design. The aim of this study was to investigate the fundamental differences in ibuprofen and diclofenac drug delivery vehicles, specifically gels and plasters, compared to a recently launched 200 mg ibuprofen medicated plaster and characterize the resulting dermatologic-pharmacokinetic profiles into and through human skin layers. skin permeation testing over 24 hours and sacrificial timepoint penetration experiments (at 1, 4, 8, 12, and 24 hours) were conducted using an automated flow-through diffusion cell system. Read More

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Optical Flow Ratio for Assessing Stenting Result and Physiological Significance of Residual Disease.

EuroIntervention 2021 Jun 8. Epub 2021 Jun 8.

Biomedical Instrument Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.

Background: Optical flow ratio (OFR) is a novel method for fast computation of fractional flow reserve (FFR) from optical coherence tomography (OCT) images.

Aims: We aimed to evaluate the accuracy of OFR in predicting post-percutaneous coronary intervention (PCI) FFR and to evaluate the impact of stent expansion on within-stent OFR pressure drop (In-stent OFR).

Methods: Post-PCI OFR was computed in patients with both OCT and FFR interrogation immediately after PCI. Read More

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The dynamics of stem water storage in the tops of Earth's largest trees-Sequoiadendron giganteum.

Tree Physiol 2021 Jun 8. Epub 2021 Jun 8.

Department of Integrative Biology, University of California, Berkeley, California 94720, USA.

Water stored in tree stems (i.e., trunks and branches) is an important contributor to transpiration that can improve photosynthetic carbon gain and reduce the probability of cavitation. Read More

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Aortic flow dynamics and stiffness in Loeys-Dietz syndrome patients: a comparison with healthy volunteers and Marfan syndrome patients.

Eur Heart J Cardiovasc Imaging 2021 Jun 9. Epub 2021 Jun 9.

Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain.

Aims: To assess aortic flow and stiffness in patients with Loeys-Dietz syndrome (LDS) by 4D flow and cine cardiovascular magnetic resonance (CMR) and compare the results with those of healthy volunteers (HV) and Marfan syndrome (MFS) patients.

Methods And Results: Twenty-one LDS and 44 MFS patients with no previous aortic dissection or surgery and 35 HV underwent non-contrast-enhanced 4D flow CMR. In-plane rotational flow (IRF), systolic flow reversal ratio (SFRR), and aortic diameters were obtained at 20 planes from the ascending (AAo) to the proximal descending aorta (DAo). Read More

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Dynamics of HIV reservoir decay and naïve CD4 T-cell recovery between immune non-responders and complete responders on long-term antiretroviral treatment.

Clin Immunol 2021 Jun 6;229:108773. Epub 2021 Jun 6.

Treatment and Research Center for Infectious Diseases, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China. Electronic address:

Background: The dynamics of viral reservoir decay and naïve CD4 T-cell recovery between immunological non-responders (INR) and complete responders (CR) during long-term antiretroviral treatment (ART) are not fully known.

Methods: Twenty-eight chronic HIV-infected individuals on 5-year ART were divided into two groups: INR (CD4 counts ≤350 cells/μL, n = 13) and CR (CD4 counts ≥500 cells/μL, n = 15). The levels of HIV DNA and cell-associated HIV RNA (CA-RNA), CD4 counts, naïve CD4 counts and their correlations were analyzed at baseline, years 1, 3 and 5 of ART between the two groups. Read More

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Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis.

Immun Inflamm Dis 2021 Jun 8. Epub 2021 Jun 8.

Department of Neurosurgery of the First Affiliated Hospital, Jiamusi University, Jiamusi, China.

Background: Severe carotid stenosis is a common cause of stroke. In addition, previous clinical studies revealed that patients symptomatic of carotid stenosis suffer from increased episodes of stroke compared with their asymptomatic counterparts. However, the mechanism underlying these differences in the recurrence of stroke remains unclear. Read More

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Solvent Vapor Annealing, Defect Analysis, and Optimization of Self-Assembly of Block Copolymers Using Machine Learning Approaches.

ACS Appl Mater Interfaces 2021 Jun 8. Epub 2021 Jun 8.

Department of Chemistry, University of Alberta, 11227-Saskatchewan Drive, Edmonton, Alberta T6G 2G2, Canada.

Self-assembly of block copolymers (BCPs) is an alternative patterning technique that promises high resolution and density multiplication with lower costs. The defectivity of the resulting nanopatterns remains too high for many applications in microelectronics and is exacerbated by small variations of processing parameters, such as film thickness, and fluctuations of solvent vapor pressure and temperature, among others. In this work, a solvent vapor annealing (SVA) flow-controlled system is combined with design of experiments (DOE) and machine learning (ML) approaches. Read More

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Flow Index: a novel, non-invasive, continuous, quantitative method to evaluate patient inspiratory effort during pressure support ventilation.

Crit Care 2021 06 7;25(1):196. Epub 2021 Jun 7.

Department of Anesthesia and Intensive Care, Fondazione Poliambulanza, Brescia, Italy.

Background: The evaluation of patient effort is pivotal during pressure support ventilation, but a non-invasive, continuous, quantitative method to assess patient inspiratory effort is still lacking. We hypothesized that the concavity of the inspiratory flow-time waveform could be useful to estimate patient's inspiratory effort. The purpose of this study was to assess whether the shape of the inspiratory flow, as quantified by a numeric indicator, could be associated with inspiratory effort during pressure support ventilation. Read More

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Circular RNA circLDB2 functions as a competing endogenous RNA to suppress development and promote cisplatin sensitivity in non-squamous non-small cell lung cancer.

Thorac Cancer 2021 Jun 6. Epub 2021 Jun 6.

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Circular RNAs (circRNAs) are covalently closed RNAs and are implicated in the development of non-small cell lung cancer (NSCLC). Here, we identified the precise actions of circRNA LIM domain binding 2 (circLDB2, hsa_circ_0069244) in non-squamous NSCLC development and drug sensitivity.

Methods: CircLDB2, microRNA (miR)-346, and LIM and calponin-homology domains 1 (LIMCH1) were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Read More

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The Alteration of Salivary Immunoglobulin A in Autism Spectrum Disorders.

Front Psychiatry 2021 21;12:669193. Epub 2021 May 21.

Department of Orthodontics, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology, Tongji University, Shanghai, China.

Autism spectrum disorders (ASD) are neurodevelopmental disorders with changes in the gut and oral microbiota. Based on the intimate relationship between the oral microbiota and oral mucosal immunity, this study aimed to investigate changes in salivary immunoglobulin A (IgA) level in ASD and the underlying mechanism for any such changes. We recruited 36 children diagnosed with ASD and 35 normally developing children and measured their salivary IgA content using enzyme-linked immunosorbent assay (ELISA). Read More

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A procedure for in vitro evaluation of the immunosuppressive effect of mouse mesenchymal stem cells on activated T cell proliferation.

Stem Cell Res Ther 2021 Jun 5;12(1):319. Epub 2021 Jun 5.

Laboratory of Stem Cell Biology, Institute of Cellular Biology and Pathology "Nicolae Simionescu", 8 B.P. Hasdeu Street, 050568, Bucharest, Romania.

Background: Mesenchymal stem/stromal cells (MSC) represent adult cells with multipotent capacity. Besides their capacity to differentiate into multiple lineages in vitro and in vivo, increasing evidence points towards the immunomodulatory capacity of these cells, as an important feature for their therapeutic power. Although not included in the minimal criteria established by the International Society for Cellular Therapy as a defining MSC attribute, demonstration of the immunomodulatory capacity of MSC can be useful for the characterization of these cells before being considered MSC. Read More

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Effects of Obesity-Associated Chronic Inflammation on Peripheral Blood Immunophenotype Are Not Mediated by TNF in Female C57BL/6J Mice.

Immunohorizons 2021 Jun 4;5(6):370-383. Epub 2021 Jun 4.

McMaster Immunology Research Centre, McMaster University, Hamilton, Ontario, Canada;

Chronic low-grade systemic inflammation in obesity contributes to the development and progression of aspects of metabolic syndrome. In obese male mice, expanded adipose tissue releases proinflammatory cytokines, including TNF, which promotes an increase in immature, proinflammatory, peripheral blood Ly-6C monocytes. The aim of this study was to characterize how TNF alters circulating cellular immunity in female mice with diet-induced obesity. Read More

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Let it flow: Modeling ecological benefits and hydropower production impacts of banning zero-flow events in a large regulated river system.

Sci Total Environ 2021 Aug 10;783:147010. Epub 2021 Apr 10.

Department of Ecology and Environmental Science, Umeå University, 901 87 Umeå, Sweden. Electronic address:

Hydropeaking, defined as rapid and frequent changes in flow to optimize hydropower production, is an increasingly common procedure negatively affecting lotic habitats in riverine ecosystems. An important aspect of hydropeaking is zero-flow events, occurring when hydropower stations are stopped due to low energy demand or low electricity prices. We quantified the ecological benefits and consequences for hydropower production of restricting zero-flow events. Read More

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Quantitative circular flow immunoassays with trained object recognition to detect antibodies to SARS-CoV-2 membrane glycoprotein.

Biochem Biophys Res Commun 2021 Aug 29;565:8-13. Epub 2021 May 29.

Department of Biology, State University, San Diego, USA. Electronic address:

Amidst infectious disease outbreaks, a practical tool that can quantitatively monitor individuals' antibodies to pathogens is vital for disease control. The currently used serological lateral flow immunoassays (LFIAs) can only detect the presence of antibodies for a single antigen. Here, we fabricated a multiplexed circular flow immunoassay (CFIA) test strip with YOLO v4-based object recognition that can quickly quantify and differentiate antibodies that bind membrane glycoprotein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or hemagglutinin of influenza A (H1N1) virus in the sera of immunized mice in one assay using one sample. Read More

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