87 results match your criteria quantified ergosterol

Whole genome sequencing of a clinical drug resistant isolate reveals known and novel mutations in genes involved in resistance acquisition mechanisms.

J Med Microbiol 2021 Apr;70(4)

Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic.

is an opportunistic pathogen accounting for the majority of cases of infections. Currently, are developing resistance towards different classes of antifungal drugs and this has become a global health burden that does not spare Lebanon. This study aims at determining point mutations in genes known to be involved in resistance acquisition and correlating resistance to virulence and ergosterol content in the azole resistant isolate CA77 from Lebanon. Read More

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Potentiality of Native Ascomycete Strains in Bioremediation of Highly Polychlorinated Biphenyl Contaminated Soils.

Microorganisms 2021 Mar 16;9(3). Epub 2021 Mar 16.

Laboratoire d'Ecologie Alpine, UMR 5553 CNRS/USMB Université Grenoble Alpes, CEDEX 9, 38058 Grenoble, France.

Polychlorinated biphenyls (PCBs) are organic pollutants that are harmful to environment and toxic to humans. Numerous studies, based on basidiomycete strains, have reported unsatisfactory results in the mycoremediation of PCB-contaminated soils mainly due to the non-telluric origin of these strains. The abilities of a five-Ascomycete-strain consortium in the mycoremediation of PCB-polluted soils and its performance to restore their sound functioning were investigated using mesocosm experiments associated with chromatography gas analysis and enzymatic activity assays. Read More

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Production and turnover of mycorrhizal soil mycelium relate to variation in drought conditions in Mediterranean Pinus pinaster, Pinus sylvestris and Quercus ilex forests.

New Phytol 2021 05 29;230(4):1609-1622. Epub 2020 Nov 29.

Joint Research Unit CTFC - AGROTECNIO, Av. Alcalde Rovira Roure 191, Lleida, 25198, Spain.

In forests, ectomycorrhizal mycelium is pivotal for driving soil carbon and nutrient cycles, but how ectomycorrhizal mycelial dynamics vary in ecosystems with drought periods is unknown. We quantified the production and turnover of mycorrhizal mycelium in Mediterranean Pinus pinaster, Pinus sylvestris and Quercus ilex forests and related the estimates to standardised precipitation index (SPI), to study how mycelial dynamics relates to tree species and drought-moisture conditions. Production and turnover of mycelium was estimated between July and February, by quantifying the fungal biomass (ergosterol) in ingrowth mesh bags and using statistical modelling. Read More

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Characterization of seven sterols in five different types of cattle feedstuffs.

Food Chem 2021 Mar 27;340:127926. Epub 2020 Aug 27.

Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro, 5, 00185 Rome, Italy.

This paper provides a method for the quantification of sterols in different types of calf feedstuffs based on soy, sunflower, hay, calf feed and a mixture of all of them. The free fraction and the total sterolic fraction, after saponification and acidic hydrolysis of the samples, are extracted by solvent and the sterols are identified/quantified by reversed phase HPLC coupled to tandem mass spectrometry by atmospheric pressure chemical ionization. After the recovery evaluation, the method is validated in terms of linearity (coefficient of determination R), repeatability (coefficient of variation RSD), limit of detection and quantification. Read More

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The Fungicide Tebuconazole Confounds Concentrations of Molecular Biomarkers Estimating Fungal Biomass.

Bull Environ Contam Toxicol 2020 Oct 28;105(4):620-625. Epub 2020 Aug 28.

iES Landau, Institute for Environmental Sciences, University of Koblenz-Landau, Fortstraße 7, 76829, Landau, Germany.

Due to their ecological importance, fungi are suitable indicator organisms for anthropogenic stress. To estimate fungal biomass, the fungal membrane molecule ergosterol is often quantified as a proxy. Estimates based on ergosterol may, however, be distorted by exposure to demethylase inhibiting (DMI) fungicides, interfering with sterol synthesis. Read More

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October 2020

Three-Dimensional Study of Colonisation of Stored Wheat: Post-Harvest Growth Patterns, Dry Matter Losses and Mycotoxin Contamination.

Microorganisms 2020 Aug 1;8(8). Epub 2020 Aug 1.

Applied Mycology Group, School of Water, Energy and Environment, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK.

causes significant post-harvest quality losses and mycotoxin contamination in stored wheat but the colonisation dynamics of the grain and how this may be affected by the initial inoculum position in the grain mass is poorly understood. This study examined the 3D growth kinetics and mycotoxin production (deoxynivalenol and zearalenone) by during hyphal colonisation from different initial inoculum positions in wheat microcosms (top-centre, bottom-centre, and bottom-side) maintained at two water activities (a; 0.95 and 0. Read More

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Antifungal In Vitro Activity of Pilosulin- and Ponericin-Like Peptides from the Giant Ant and Synergistic Effects with Antimycotic Drugs.

Antibiotics (Basel) 2020 Jun 23;9(6). Epub 2020 Jun 23.

Postgraduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza 60416-030, Brazil.

Venoms from ants comprise a rich source of bioactive peptides, including antimicrobial peptides. From the proteome and peptidome of the giant ant venom, members of five known classes of antimicrobial peptides were disclosed (e.g. Read More

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Changes in soil fungal community composition depend on functional group and forest disturbance type.

New Phytol 2021 01 16;229(2):1105-1117. Epub 2020 Jul 16.

Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2E3, Canada.

Disturbances have altered community dynamics in boreal forests with unknown consequences for belowground ecological processes. Soil fungi are particularly sensitive to such disturbances; however, the individual response of fungal guilds to different disturbance types is poorly understood. Here, we profiled soil fungal communities in lodgepole pine forests following a bark beetle outbreak, wildfire, clear-cut logging, and salvage-logging. Read More

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January 2021

UPLC-MS/MS Identification and Quantification of Withanolides from Six Parts of the Medicinal Plant L.

Molecules 2020 Mar 11;25(6). Epub 2020 Mar 11.

Key Laboratory of Chinese Materia, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.

Withanolides from six parts (flower, leaf, stem, root, seed, and peel) of L. ( L.) obtained from ten production areas in China were identified and quantified by UPLC-MS/MS. Read More

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Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage.

Microb Biotechnol 2020 07 10;13(4):1054-1065. Epub 2020 Mar 10.

Rothamsted Research, North Wyke, Okehampton, Devon, EX20 2SB, UK.

Proliferation of filamentous fungi following ingress of oxygen to silage is an important cause of dry matter losses, resulting in significant waste. In addition, the production of mycotoxins by some filamentous fungi poses a risk to animal health through mycotoxicosis. Quantitative assessment of fungal growth in silage, through measurement of ergosterol content, colony-forming units or temperature increase is limiting in representing fungal growth dynamics during aerobic spoilage due to being deficient in either representing fungal biomass or being able to identify specific genera. Read More

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Sertraline as a promising antifungal agent: inhibition of growth and biofilm of Candida auris with special focus on the mechanism of action in vitro.

J Appl Microbiol 2020 Feb 13;128(2):426-437. Epub 2019 Nov 13.

Department of Physics, Periyar University, Salem, Tamil Nadu, India.

Aim: The aim of this present study was to investigate the antifungal mechanism of sertraline against Candida auris (C. auris) and its effect on biofilm formation.

Methods And Results: Sertraline, a repurposing drug with a history of human use for the treatment of depression was screened against three different isolates of C. Read More

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February 2020

Assessment of the and activity of atorvastatin against .

J Med Microbiol 2019 Oct 28;68(10):1497-1506. Epub 2019 Aug 28.

SSPC Research Centre, Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland.

. The aim of this work was to characterize the response of to atorvastatin, and to assess its antifungal capability.. Read More

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October 2019

Intake of ergosterol increases the vitamin D concentrations in serum and liver of mice.

J Steroid Biochem Mol Biol 2019 11 25;194:105435. Epub 2019 Jul 25.

Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Von-Danckelmann-Platz 2, 06120, Halle (Saale), Germany; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, Germany. Electronic address:

Factors that can modify the bioavailability of orally administered vitamin D are not yet widely known. Ergosterol is a common fungal sterol found in food which has a chemical structure comparable to that of vitamin D. This study aimed to investigate the effect of ergosterol on vitamin D metabolism. Read More

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November 2019

Subcritical water extraction of withanosides and withanolides from ashwagandha (Withania somnifera L) and their biological activities.

Food Chem Toxicol 2019 Oct 2;132:110659. Epub 2019 Jul 2.

Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China. Electronic address:

Subcritical water extraction (SWE) applied to analyses the bioactives from ashwagandha (W. somnifera) at varying temperature (100-200 °C) and extraction time (10-30 min). The effect of temperature and time has been investigated in terms of extraction yield (EY), total phenolic content (TPC), cytotoxicity, antioxidant, and enzyme inhibitory activities. Read More

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October 2019

The antifungal activity and mechanisms of action of quantified extracts from berries, leaves and roots of Phytolacca tetramera.

Phytomedicine 2019 Jul 16;60:152884. Epub 2019 Mar 16.

Área Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Suipacha 531, 2000 Rosario, Argentina. Electronic address:

Background: Phytolacca tetramera is an endemic plant from Argentina that is currently at serious risk because its environment is subjected to a high anthropic impact. A previous study has shown that berry extracts obtained from this plant display antifungal activity against multiple human-pathogenic fungi when tested with a non-standardized method. Further evidences of the antifungal properties of other parts of the plant and studies of mechanism of antifungal action of the antifungal chemically characterized extracts are required. Read More

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Innate endophytic fungus, Aspergillus terreus as biotic elicitor of withanolide A in root cell suspension cultures of Withania somnifera.

Mol Biol Rep 2019 Apr 31;46(2):1895-1908. Epub 2019 Jan 31.

Microbial Technology Laboratory, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Allalasandra, GKVK Post, Bangalore, 560 065, India.

In the present study, root cell suspension cultures of W. somnifera were elicited with mycelial extract (1% w/v) and culture filtrate (5% v/v) of their native endophytic fungus Aspergillus terreus 2aWF in shake flask. Culture filtrate of A. Read More

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Fusarium Species and Mycotoxins Contaminating Veterinary Diets for Dogs and Cats.

Microorganisms 2019 Jan 21;7(1). Epub 2019 Jan 21.

Department of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, Poznań 60-625, Poland.

Veterinary diets are intended for diseased animals and may contain cereal grains, mainly maize and/or wheat. These, in turn, are often infected with pathogens of the genus, which are able to produce numerous harmful mycotoxins. Forty-two samples of veterinary diets for dogs and cats were analyzed for the presence of species and mycotoxins. Read More

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January 2019

Approaches to the mechanism of antifungal activity of Zuccagnia punctata-Larrea nitida bi-herbal combination.

Phytomedicine 2019 Feb 6;54:291-301. Epub 2018 Jul 6.

Área Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Argentina. Electronic address:

Background: In our previous study the synergism of four combinations of Zuccagnia punctata (ZpE) and Larrea nitida (LnE) exudates with the reliable statistical-based MixLow method was assessed, and the markers of the most anti-C. albicans synergistic ZpE-LnE bi-herbal combination were quantified according to European Medicines Agency (EMA).

Purpose: To study the mechanisms of action as well as the cytotoxic properties of the ZpE-LnE most synergistic combination found in the previous work. Read More

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February 2019

Efficacy of Novel Schiff base Derivatives as Antifungal Compounds in Combination with Approved Drugs Against Candida Albicans.

Med Chem 2019 ;15(6):648-658

Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi-110025, India.

Background: The increasing incidence of fungal infections, especially caused by Candida albicans, and their increasing drug resistance has drastically increased in recent years. Therefore, not only new drugs but also alternative treatment strategies are promptly required.

Methods: We previously reported on the synergistic interaction of some azole and non-azole compounds with fluconazole for combination antifungal therapy. Read More

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October 2019

Withaferin-A attenuates multiple low doses of Streptozotocin (MLD-STZ) induced type 1 diabetes.

Biomed Pharmacother 2018 Oct 24;106:1428-1440. Epub 2018 Jul 24.

Department of Regulatory Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, Telangana, India. Electronic address:

Type 1 diabetes mellitus (T1DM) is one of the major metabolic disorders with life-long dependence on insulin. The present study was designed to evaluate the antioxidant and anti-diabetic potential of Withaferin A (WA), the active constituent of Withania somnifera in multiple low doses of Streptozotocin (MLD-STZ) induced T1DM. STZ (40 mg/Kg) was administered intraperitoneally (i. Read More

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October 2018

Cocoa and chocolate are sources of vitamin D.

Food Chem 2018 Dec 20;269:318-320. Epub 2018 Jun 20.

Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Germany; Competence Cluster of Cardiovascular Health and Nutrition (nutriCARD), Halle-Jena-Leipzig, Germany. Electronic address:

Cocoa beans are susceptible to fungal contamination and often contain substantial amounts of ergosterol, the precursor to vitamin D. We hypothesized that sun-drying the fermented cocoa beans might lead to the conversion of ergosterol to vitamin D. We quantified vitamin D in cocoa and cocoa-based foods by liquid chromatography-tandem mass spectrometry. Read More

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December 2018

Sterol uptake and sterol biosynthesis act coordinately to mediate antifungal resistance in Candida glabrata under azole and hypoxic stress.

Mol Med Rep 2018 May 9;17(5):6585-6597. Epub 2018 Mar 9.

Clinical Mycology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Pathogenic fungi, including Candida glabrata, develop strategies to grow and survive both in vitro and in vivo under azole stress. However, the mechanisms by which yeast cells counteract the inhibitory effects of azoles are not completely understood. In the current study, it was demonstrated that the expression of the ergosterol biosynthetic genes ERG2, ERG3, ERG4, ERG10, and ERG11 was significantly upregulated in C. Read More

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Yeast membrane lipid imbalance leads to trafficking defects toward the Golgi.

Cell Biol Int 2018 Jul 19;42(7):890-902. Epub 2018 Apr 19.

Missouri State University, 901 S National Ave., Springfield, Missouri.

Protein recycling is an essential cellular process involving endocytosis, intracellular trafficking, and exocytosis. In mammalian systems membrane lipids, including cholesterol, sphingolipids, and phospholipids, play a pivotal role in protein recycling. To address this role in budding yeast, Saccharomyces cerevisiae, we utilized GFP-Snc1, a v-SNARE protein serving as a fluorescent marker for faithfully reporting the recycling pathway. Read More

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Anti-tumor and Anti-angiogenic Ergosterols from .

Front Chem 2017 30;5:85. Epub 2017 Oct 30.

Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, China.

This study was carried out to isolate chemical constituents from the lipid enriched fraction of extract and to evaluate their anti-proliferative effect on tumor cells and human umbilical vein endothelial cells (HUVECs). Ergosterol derivatives (-) were isolated and purified from the lipid enriched fraction of . Their chemical structures were established by spectroscopic analyses or by comparison of mass and NMR spectral data with those reported previously. Read More

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October 2017

UV-based evaluation of ergosterol for monitoring the fungal exposure in Italian buffalo farms.

FEMS Microbiol Lett 2017 Dec;364(22)

Istituto di Biologia Agro-ambientale e Forestale (IBAF), Consiglio Nazionale delle Ricerche (CNR), via P. Castellino 111, 80128, Napoli, Italy.

This study provided a reliable way to identify and estimate the ergosterol in farm environments, since it is renowned that it is a specific indicator for the occurrence of molds and yeasts. The quick valuation of exposure to airborne microorganisms is essential to assess the risk to which the health of employees is subjected in working places characterized by great humidity. From this view, it is worth estimating the total biomass of molds and yeast, including viable and non-viable forms, which may cause respiratory concerns to human. Read More

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December 2017

Withanolide production by fungal endophyte isolated from Withania somnifera.

Nat Prod Res 2018 Jul 16;32(13):1573-1577. Epub 2017 Oct 16.

a Department of Botany , Bharathidasan University , Tiruchirappalli , India.

Withanolides, the secondary metabolite from Withania species is used in Ayurvedic medicine and now proved to have potential use in treating cardiovascular, Alzheimer's disease, etc. Its production in plants varies between genotypes with very low yield. For improved industrial commercialisation, there is a need to increase its production. Read More

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The Effectiveness of Voriconazole in Therapy of Candida glabrata's Biofilms Oral Infections and Its Influence on the Matrix Composition and Gene Expression.

Mycopathologia 2017 Aug 24;182(7-8):653-664. Epub 2017 Apr 24.

CEB, Centre of Biological Engineering, LIBRO - Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, 4710-057, Braga, Portugal.

Candida glabrata is one of most prevalent yeast in fungal infections, especially in immunocompromised patients. Its azole resistance results in a low therapeutic response, particularly when associated with biofilms. The main goal of this work was to study the effectiveness of voriconazole (Vcz) against C. Read More

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A UHPLC method for the rapid separation and quantification of phytosterols using tandem UV/Charged aerosol detection - A comparison of both detection techniques.

J Pharm Biomed Anal 2017 Jun 30;140:274-280. Epub 2017 Mar 30.

The Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, Hradec Králové 500 05, Czech Republic.

The presented work describes the development and validation of a rapid UHPLC-UV/CAD method using a core-shell particle column for the separation and quantitative analysis of seven plant sterols and stanols. The phytosterols (ergosterol, brassicasterol, campesterol, fucosterol, stigmasterol, and β-sitosterol) and the phytostanol stigmastanol were separated and analyzed in 8.5min. Read More

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Positive and negative aspects of green coffee consumption - antioxidant activity versus mycotoxins.

J Sci Food Agric 2017 Sep 10;97(12):4022-4028. Epub 2017 Mar 10.

Institute of Chemistry and Technical Electrochemistry, Poznan University of Technology, Poznań, Poland.

Background: The quality of coffee depends not only on the contents of healthy compounds but also on its contamination with microorganisms that can produce mycotoxins during development, harvesting, preparation, transport and storage.

Results: The antioxidant activity of green coffee brews measured in this study by ABTS, DPPH and Folin-Ciocalteu assays showed that coffee extracts from Robusta beans possessed higher activity in all assays than extracts from Arabica beans. The occurrence of ochratoxin A and aflatoxins (B1, B2, G1 and G2) in green coffee beans was studied using liquid chromatography/mass spectrometry. Read More

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September 2017

Changes in turnover rather than production regulate biomass of ectomycorrhizal fungal mycelium across a Pinus sylvestris chronosequence.

New Phytol 2017 Apr 20;214(1):424-431. Epub 2016 Dec 20.

School of Science and Technology, Örebro University, Örebro, SE-701 82, Sweden.

In boreal forest soils, ectomycorrhizal fungi are fundamentally important for carbon (C) dynamics and nutrient cycling. Although their extraradical mycelium (ERM) is pivotal for processes such as soil organic matter build-up and nitrogen cycling, very little is known about its dynamics and regulation. In this study, we quantified ERM production and turnover, and examined how these two processes together regulated standing ERM biomass in seven sites forming a chronosequence of 12- to 100-yr-old managed Pinus sylvestris forests. Read More

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