71 results match your criteria quantification gabapentin

Development and Validation of 2-Azaspiro [4,5] Decan-3-One (Impurity A) in Gabapentin Determination Method Using qNMR Spectroscopy.

Molecules 2021 Mar 16;26(6). Epub 2021 Mar 16.

"Scientific Centre for Expert Evaluation of Medicinal Products" of the Ministry of Health of the Russian Federation, Federal State Budgetary Institution, 8/2 Petrovsky Blvd, 127051 Moscow, Russia.

The authors developed a H qNMR test procedure for identification and quantification of impurity A present in gabapentin active pharmaceutical ingredient (API) and gabapentin products. The validation studies helped to determine the limit of quantitation and assess linearity, accuracy, repeatability, intermediate precision, specificity, and robustness of the procedure. Spike-and-recovery assays were used to calculate standard deviations, coefficients of variation, confidence intervals, bias, Fisher's test, and Student's -test for assay results. Read More

View Article and Full-Text PDF

Effect of gabapentin on hyperemesis gravidarum: a double-blind, randomized controlled trial.

Am J Obstet Gynecol MFM 2021 01 29;3(1):100273. Epub 2020 Oct 29.

Department of Obstetrics and Gynecology, University of Rochester, Rochester, NY.

Background: Hyperemesis gravidarum is a disabling disease of nausea, vomiting, and undernutrition in early pregnancy for which there are no effective outpatient therapies. Poor weight gain in hyperemesis gravidarum is associated with several adverse fetal outcomes including preterm delivery, low birthweight, small for gestational age, low 5-minute Apgar scores, and neurodevelopmental delay. Gabapentin is most commonly used clinically for treating neuropathic pain but also substantially reduces chemotherapy-induced and postoperative nausea and vomiting. Read More

View Article and Full-Text PDF
January 2021

Development of an analytical method to quantify pharmaceuticals in fish tissues by liquid chromatography-tandem mass spectrometry detection and application to environmental samples.

J Chromatogr A 2020 Dec 12;1633:461612. Epub 2020 Oct 12.

Federal Institute of Hydrology, Am Mainzer Tor 1, Koblenz 56068, Germany. Electronic address:

A sensitive multiresidue method was developed to quantify 35 pharmaceuticals and 28 metabolites/transformation products (TPs) in fish liver, fish fillet and fish plasma via LC-MS/MS. The method was designed to cover a broad range of substance polarities. This objective was realized by using non-discriminating sample clean-ups including separation technique based on size exclusion, namely restricted access media (RAM) chromatography. Read More

View Article and Full-Text PDF
December 2020

Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary.

Talanta 2021 Jan 8;221:121626. Epub 2020 Sep 8.

Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo n. 2, 166 10, Prague 6, Czech Republic. Electronic address:

Electrophoretic stacking is developed for sensitive determination of three zwitterionic antiepileptics, namely vigabatrin, pregabalin and gabapentin, in human serum. CE separation is performed in a 25 μm fused silica capillary covalently coated with the copolymer of acrylamide with 5% content of permanently charged 3-acrylamidopropyl trimethylammonium chloride (PAMAPTAC). In background electrolyte of 500 mM acetic acid, the 5% PAMAPTAC generates an anodic electro-osmotic flow with a magnitude of (-18. Read More

View Article and Full-Text PDF
January 2021

Distribution of Fluvoxamine and Identification of the Main Metabolite in a Fatal Intoxication.

J Anal Toxicol 2021 Apr;45(4):e1-e5

Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini, 12, 27100 Pavia, Italy.

Fluvoxamine is a selective serotonin reuptake inhibitor, with a half-life of about 30 hours, that is commonly prescribed in the treatment of depression and obsessive and compulsive disorders. Though its more favorable adverse effect profile in comparison to tricyclic antidepressants, overdosages could lead to severe central nervous system depression. We hereby report the case of a 48-year-old woman with psychiatric disorders, who died in the Protected Community where she lived. Read More

View Article and Full-Text PDF

Fast simultaneous quantification of gabapentin and cetirizine in cell lysates by means of HPLC-MS/MS.

J Pharm Biomed Anal 2020 May 13;184:113172. Epub 2020 Feb 13.

University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstraße 28/30, 48149 Münster, Germany. Electronic address:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was successfully developed for the simultaneous detection of gabapentin (GBP) and cetirizine (CTZ) in cell lysates. Multiple reaction monitoring (MRM) transitions were found for GBP, CTZ and deuterated internal standards of both, allowing a selective and sensitive detection. Limits of detection (LODs) of 0. Read More

View Article and Full-Text PDF

Enduring alterations in hippocampal astrocytesynaptic proximity following adolescent alcohol exposure: reversal by gabapentin.

Neural Regen Res 2020 Aug;15(8):1496-1501

Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC, USA.

Adolescent alcohol abuse is a substantive public health problem that has been the subject of intensive study in recent years. Despite reports of a wide range of effects of adolescent intermittent ethanol (AIE) exposure on brain and behavior, little is known about the mechanisms that may underlie those effects, and even less about treatments that might reverse them. Recent studies from our laboratory have indicated that AIE produced enduring changes in astrocyte function and synaptic activity in the hippocampal formation, suggesting the possibility of an alteration in astrocyte-neuronal connectivity and function. Read More

View Article and Full-Text PDF

An Insight into Gabapentin and Pregabalin in Scottish Prisoners.

J Anal Toxicol 2020 Apr;44(5):504-513

Forensic Medicine and Science, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK.

The aim of this study was to evaluate the prevalence and abuse potential of antiepileptic drugs (AEDs) among prison populations in Scotland, UK. Participants consisted of all admitted and released prisoners over a 1 month period who consented to provide samples. Urine samples were collected and analyzed by liquid chromatography coupled with triple quadrupole tandem mass spectrometry using a method validated for the simultaneous quantification of 21 AEDs in urine. Read More

View Article and Full-Text PDF

Validation of a High-throughput Screening and Quantification Method for the Determination of Gabapentinoids in Blood Using a Combination of LC-TOF-MS and LC-MS-MS.

J Anal Toxicol 2019 Oct;43(9):696-702

Department of Forensic Toxicology, New York City Office of Chief Medical Examiner, New York City, NY 10016 USA.

Gabapentinoids such as gabapentin (GP) and pregabalin (PGL) have been used to treat a wide range of neurological and psychiatric disorders. In recent years, there has been an increasing awareness of GP and PGL misuse among individuals with a history of polysubstance use. Both GP and PGL are understood to potentiate the effects of opioids, with fatalities involving GP and PGL being reported with increasing frequency. Read More

View Article and Full-Text PDF
October 2019

LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction.

Int J Anal Chem 2018 28;2018:1605950. Epub 2018 Oct 28.

College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA.

An accurate, highly sensitive, and precise method for quantitative analysis of tramadol (TMD) and gabapentin (GBP) by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using venlafaxine and pregabalin as internal standards (ISTDs), respectively. An aliquot of 200 L of plasma was mixed with internal standard dilution and extraction was performed by using solid phase extraction (SPE) technique. Peak resolution was achieved on Phenomenex PFP column (50×4. Read More

View Article and Full-Text PDF
October 2018

Differential Expression of Phosphorylated ERK and c-Fos of Limbic Cortices Activities in Response to Tactile Allodynia of Neuropathic Rats.

Chin J Physiol 2018 Aug;61(4):240-251

Department of Life Science, National Taiwan University, Taipei 10617, Taiwan, Republic of China.

Neuropathic pain is due to lesion or dysfunction of the somatosensory system. Treating patients with neuropathic pain is difficult because the underlying mechanisms are understood limitedly, especially at the supraspinal level. In this study, we used two kinds of molecular markers to investigate the neuronal activity changes in the anterior cingulate cortex, insular cortex (IC), and medial prefrontal cortex (mPFC) of the neuropathic rats under tactile allodynia. Read More

View Article and Full-Text PDF

Development and Validation of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Concurrent Measurement of Gabapentin, Lamotrigine, Levetiracetam, Monohydroxy Derivative of Oxcarbazepine, and Zonisamide Concentrations in Serum in a Clinical Setting.

Ther Drug Monit 2018 08;40(4):469-476

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School.

Background: Therapeutic drug monitoring of antiepileptic drugs (AEDs) is often necessary to prevent associated destructive toxicities. Tandem mass spectrometry (MS/MS) with stable-isotope-labeled internal standards is considered the gold standard for the measurement of AEDs. This study presents the development and validation of a clinical ultra-performance liquid chromatography-MS/MS method for the concurrent measurement of gabapentin, lamotrigine, levetiracetam, monohydroxy derivative of oxcarbazepine, and zonisamide in human serum. Read More

View Article and Full-Text PDF

Development and validation of a hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the quantification of the antidiabetic drug metformin and six others pharmaceuticals in wastewater.

J Chromatogr A 2018 Jun 4;1556:73-80. Epub 2018 May 4.

Institute of Clinical Pharmacology, Medical Faculty, Technical University Dresden, Germany.

A rapid and sensitive hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry method was developed and fully validated for the simultaneous determination of the antidiabetic drug metformin and six further pharmaceuticals (atenolol, gabapentin, levofloxacin, ciprofloxacin, propranolol and trimethoprim) in influent and effluent wastewater. Five deuterated related compounds were used as internal standards in order to control the extraction, injection and ionization variability. Two solid phase extraction methods and Oasis HLB 1cc cartridge, using only 1mL sample with acid or basic pH-value were optimized and compared in order to match the specificity of both influent and effluent wastewater matrixes. Read More

View Article and Full-Text PDF

Utilization of gabapentin by people in treatment for substance use disorders in Belgium (2011-2014): a cross-sectional study.

Arch Public Health 2018 19;76:17. Epub 2018 Mar 19.

Department of Public Health and Surveillance, Scientific Institute of Public Health, Brussels, Belgium, Rue Juliette Wytsmanstraat, 14, 1050 Brussels, Belgium.

Background: Although gabapentin has been licensed in the European Union only for neuropathic pain and epilepsy for patients who have partial seizures, it has also been prescribed in treatment for substance use disorders. Many studies report the potential risk of abuse of gabapentin by people with substance use disorders. The objective of this paper is to determine if people who have been in treatment for substance use disorders bought gabapentin in a time span that could indicate consumption at a dose that exceeded the maximum approved dose of 3600 mg/day. Read More

View Article and Full-Text PDF

Substrate Selectivity Check of the Ergothioneine Transporter.

Drug Metab Dispos 2018 Jun 12;46(6):779-785. Epub 2018 Mar 12.

Department of Pharmacology, University of Cologne, Cologne, Germany

The candidate vitamin ergothioneine (ET) is a unique antioxidant. Expression of the ET transporter (ETT) (gene symbol ) in distinct cells is thought to signal intracellular ET activity, since we have previously shown that the ETT is highly selective for ET. Unfortunately, some continue to hold the ETT as a relevant drug transporter, using the misleading functional name OCTN1, novel organic cation transporter. Read More

View Article and Full-Text PDF

Gabapentin reduces haloperidol-induced vacuous chewing movements in mice.

Pharmacol Biochem Behav 2018 03 31;166:21-26. Epub 2018 Jan 31.

Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil. Electronic address:

Tardive dyskinesia (TD) is a common adverse effect observed in patients with long-term use of typical antipsychotic medications. A vacuous chewing movement (VCM) model induced by haloperidol has been used to study these abnormalities in experimental animals. The cause of TD and its treatment remain unknown, but several lines of evidence suggest that dopamine receptor supersensitivity and gamma-aminobutyric acid (GABA) insufficiency play important roles in the development of TD. Read More

View Article and Full-Text PDF

An HPLC tandem mass spectrometry for quantification of ET-26-HCl and its major metabolite in plasma and application to a pharmacokinetic study in rats.

J Pharm Biomed Anal 2018 Feb 8;149:381-386. Epub 2017 Nov 8.

Department of Clinical Pharmacy and Pharmacy Administration, Key Laboratory of Drug Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, Sichuan, China. Electronic address:

ET-26-HCl is a new analog of etomidate, a short-acting anesthetic drug, with less adrenal cortex inhibition. The pharmacokinetics of ET-26-HCl in rats needs to be determined for future clinical trials in human subjects. In order to facilitate the pharmacokinetic study, a liquid chromatography based tandem mass spectrometric (HPLC-MS/MS) method was developed and validated for quantification of ET-26-HCl and its major metabolite, ET-26-acid. Read More

View Article and Full-Text PDF
February 2018

Quantification of the Pharmacodynamic Interaction of Morphine and Gabapentin Using a Response Surface Approach.

AAPS J 2017 11 29;19(6):1804-1813. Epub 2017 Aug 29.

Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark.

The combination of morphine and gabapentin has shown to be promising for managing postoperative pain but finding the right dose for the combination has proven to be a challenge. The purpose of this study was to quantitatively characterize the pharmacodynamic interaction between the two drugs and to identify the optimal concentration-effect relationship of the combination. Information regarding plasma concentrations and von Frey withdrawal thresholds following incisional surgery on Sprague Dawley rats, after administration of morphine, gabapentin, or their combination was available from published studies. Read More

View Article and Full-Text PDF
November 2017

Quantification of gabapentin polymorphs in gabapentin/excipient mixtures using solid state C NMR spectroscopy and X-ray powder diffraction.

J Pharm Biomed Anal 2017 Nov 5;146:29-36. Epub 2017 Aug 5.

Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, USA. Electronic address:

Gabapentin was used as a model pharmaceutical compound with susceptibility to polymorphic transformation as a function of environmental and mechanical stress. The utility of C CP/MAS NMR and XRPD as stability-indicating methods to quantify polymorphic transformation kinetics was investigated. Polymorphic Form II and III were distinguishable based on their chemical shift and distinct diffraction peak differences. Read More

View Article and Full-Text PDF
November 2017

Quantification of Quercetin Obtained from Lam. Leaves and its Effects on Streptozotocin-induced Diabetic Neuropathy.

Pharmacognosy Res 2017 Jul-Sep;9(3):287-293

Department of Pharmacology, Y B Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Aurangabad, Maharashtra, India.

Objective: Antioxidant potential has protective effects in diabetic neuropathy (DN); hence, the present study was designed with an objective to quantify quercetin from shade-dried leaves of Lam. and to study its effects on streptozotocin (STZ)-induced chronic DN.

Materials And Methods: The shade-dried leaves of Lam. Read More

View Article and Full-Text PDF

Spectroscopic Methods for Quantifying Gabapentin: Framing the Methods without Derivatization and Application to Different Pharmaceutical Formulations.

Appl Spectrosc 2017 Nov 29;71(11):2519-2531. Epub 2017 Jun 29.

1 Departamento de Química e Farmácia, FCT, Universidade do Algarve, Faro, Portugal.

This work aimed at analyzing the performance of direct spectroscopic methods for the quantification of gabapentin (GABAp), given the lack of previous studies, in comparison with the more reviewed and complex derivatization techniques, discussing their susceptibility to the pharmaceutical formulations. All of the methods analyzed showed high selectivity for this pharmaceutical analyte, with recoveries close to 100%. Absorption spectroscopy without derivatization yielded better sensitivity and lower limits of detection and quantification of gabapentin in aqueous solution (AqSol method) when compared with other solvents, such as acidic solution or ethanol/water mixture. Read More

View Article and Full-Text PDF
November 2017

Validated Method for the Screening and Quantification of Baclofen, Gabapentin and Pregabalin in Human Post-Mortem Whole Blood Using Protein Precipitation and Liquid Chromatography-Tandem Mass Spectrometry.

J Anal Toxicol 2017 Jun;41(5):441-450

Toxicology Unit, Imperial College London, London, United Kingdom of Great Britain and Northern Ireland.

There has been a rapid increase in the number of prescriptions for baclofen (BLF), gabapentin (GBP) and pregabalin (PGL) in the UK since their introduction to therapy. Recent studies across the European Union and USA have shown the illicit abuse potential of these drugs and deaths have been observed. A simple, reliable and fully validated method was developed for the screening and quantification of BLF, GBP and PGL in human post-mortem (PM) blood. Read More

View Article and Full-Text PDF

An LC-MS/MS method for the simultaneous determination of 15 antipsychotics and two metabolites in hair and its application to rat hair.

Forensic Sci Int 2017 May 12;274:91-98. Epub 2017 Jan 12.

National Forensic Service, 10 Ipchoon-ro, Wonju, Gangwon-do, South Korea. Electronic address:

In recent years, the inappropriate use of antipsychotics by young Korean men has become a social problem. As military service exemptions are given for mental illness, some men pose as mental health patients to avoid military service. In order to verify the authenticity of mental illnesses, we developed simultaneous analytical methods for the detection of 15 antipsychotics and 2 of their metabolites in hair using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Read More

View Article and Full-Text PDF

Targeted analysis of 116 drugs in hair by UHPLC-MS/MS and its application to forensic cases.

Drug Test Anal 2017 Aug 22;9(8):1137-1151. Epub 2016 Dec 22.

Section of Forensic Chemistry, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.

A multi-target method that can detect a broad range of drugs in human hair, such as hypnotics, anxiolytics, analgesics, benzodiazepines, antihistamines, antidepressants, antipsychotics, and anticonvulsants, was developed based on ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The drugs were extracted from 10 mg of washed hair by incubation for 18 h in a 25:25:50 (v/v/v) mixture of methanol/acetonitrile/2 mM ammonium formate (8% acetonitrile, pH 5.3). Read More

View Article and Full-Text PDF

An Updated Overview on Therapeutic Drug Monitoring of Recent Antiepileptic Drugs.

Drugs R D 2016 Dec;16(4):303-316

Department of Pharmaceutics, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia.

Given the distinctive characteristics of both epilepsy and antiepileptic drugs (AEDs), therapeutic drug monitoring (TDM) can make a significant contribution to the field of epilepsy. The measurement and interpretation of serum drug concentrations can be of benefit in the treatment of uncontrollable seizures and in cases of clinical toxicity; it can aid in the individualization of therapy and in adjusting for variable or nonlinear pharmacokinetics; and can be useful in special populations such as pregnancy. This review examines the potential for TDM of newer AEDs such as eslicarbazepine acetate, felbamate, gabapentin, lacosamide, lamotrigine, levetiracetam, perampanel, pregabalin, rufinamide, retigabine, stiripentol, tiagabine, topiramate, vigabatrin, and zonisamide. Read More

View Article and Full-Text PDF
December 2016

Dried blood spot analysis of gabapentin as a valid alternative for serum: a bridging study.

J Pharm Biomed Anal 2017 Jan 26;132:72-76. Epub 2016 Sep 26.

Laboratory of Toxicology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Electronic address:

We evaluated the applicability of a validated GC-MS method for the determination of gabapentin in dried blood spots (DBS). Important for the acceptance of DBS sampling as an alternative sampling strategy is the possibility to base solid conclusions on the quantification. Therefore, bridging studies -studies in which the correlation between both DBS and a reference matrix (e. Read More

View Article and Full-Text PDF
January 2017

Gabapentin, Pregabalin and Vigabatrin Quantification in Human Serum by GC-MS After Hexyl Chloroformate Derivatization.

J Anal Toxicol 2016 Nov 1;40(9):749-753. Epub 2016 Sep 1.

Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, 121 08 Prague 2, Czech Republic

A simple, sensitive and robust method for simultaneous determination of antiepileptic drugs (gabapentin, pregabalin and vigabatrin) in human serum using GC-MS was developed and validated for clinical toxicology purposes. This method employs an emerging class of derivatization agents - alkyl chloroformates allowing the efficient and rapid derivatization of both the amino and carboxylic groups of the tested antiepileptic drugs within seconds. The derivatization protocol was optimized using the Design of Experiment statistical methodology, and the entire sample preparation requires less than 5 min. Read More

View Article and Full-Text PDF
November 2016

A Validated Fluorometric Method for the Rapid Determination of Pregabalin in Human Plasma Applied to Patients With Pain.

Ther Drug Monit 2016 10;38(5):628-33

Department of Hospital Pharmacy, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan.

Background: Pregabalin has been used for the treatment of pain. A clinically accepted method applied to patients with pain has not been published for the determination of pregabalin in human plasma. This study developed a fluorometric ultrahigh-performance liquid chromatography (UHPLC) method to measure pregabalin concentration in patients with pain. Read More

View Article and Full-Text PDF
October 2016

Development of a multi-residue method for scrutinizing psychotropic compounds in natural waters.

J Chromatogr B Analyt Technol Biomed Life Sci 2017 Mar 7;1047:160-172. Epub 2016 Jul 7.

Laboratoire National de métrologie et d'Essais (LNE), 1 rue Gaston Boissier, 75724 Paris, France.

The present work describes a multi-residue SPE-UPLC-MS/MS method aiming at the characterization of 68 compounds in natural waters, including parent compounds as well as their major metabolites and glucuronide conjugates. Development was conducted toward the quantitative determination of a broad range of analytes belonging to different class of psychotropic drugs such as benzodiazepines, antidepressants, stimulants, opiates and opioids, anticonvulsants, anti-dementia drugs, analgesic and anti-inflammatory drugs (as anthropic indicators) in the low ngL range of concentration. Satisfactory extraction recoveries >70% were obtained for the majority of analytes (49 out of 68) allowing low limits of quantification. Read More

View Article and Full-Text PDF

Quantification of new antiepileptic drugs by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to cellular uptake experiment using human placental choriocarcinoma BeWo cells.

J Chromatogr B Analyt Technol Biomed Life Sci 2015 Oct 28;1002:228-33. Epub 2015 Aug 28.

Department of Pharmacy, Hokkaido University Hospital, Kita-14-jo, Nishi-5-chome, Kita-ku, Sapporo 060-8648, Japan; Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, Nishi-6-chome, Kita-ku, Sapporo 060-0812, Japan. Electronic address:

A method for quantification of new antiepileptic drugs, including lamotrigine (LTG), levetiracetam (LEV), gabapentin (GBP), and topiramate (TPM), in cellular samples, using liquid chromatography/electrospray ionization tandem mass spectrometry was developed to better understand the membrane transport mechanisms of these drugs. Cell lysate was deproteinized by methanol containing LEV-d3 as an internal standard (IS). Chromatographic separation was performed on a C18 column using gradient elution with methanol-water-formic acid (10:90:0. Read More

View Article and Full-Text PDF
October 2015