2,110 results match your criteria quantification collagen


Extracellular Vesicles Derived from Primary Adipose Stromal Cells Induce Elastin and Collagen Deposition by Smooth Muscle Cells within 3D Fibrin Gel Culture.

Bioengineering (Basel) 2021 Apr 27;8(5). Epub 2021 Apr 27.

Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Macromolecular components of the vascular extracellular matrix (ECM), particularly elastic fibers and collagen fibers, are critical for the proper physiological function of arteries. When the unique biomechanical combination of these fibers is disrupted, or in the ultimate extreme where fibers are completely lost, arterial disease can emerge. Bioengineers in the realms of vascular tissue engineering and regenerative medicine must therefore ideally consider how to create tissue engineered vascular grafts containing the right balance of these fibers and how to develop regenerative treatments for situations such as an aneurysm where fibers have been lost. Read More

View Article and Full-Text PDF

A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells.

Int J Mol Sci 2021 Apr 14;22(8). Epub 2021 Apr 14.

Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland.

The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Read More

View Article and Full-Text PDF

Stability of housekeeping genes in inflamed joints of spontaneous and collagen-induced arthritis in DBA/1 mice.

Inflamm Res 2021 May 26;70(5):619-632. Epub 2021 Apr 26.

Laboratorio PABIOM, Facultad de Medicina y Ciencias Biomédicas, Universidad Autónoma de Chihuahua, Circuito Universitario Campus II, CP 31125, Chihuahua, Chihuahua, Mexico.

Background: DBA/1 mice arthritis models have contributed to our understanding of human rheumatoid arthritis (RA) and spondyloarthritis (SpA) pathogenesis, as well as the exploration of therapeutic targets for treatment. Quantitative polymerase chain reaction (qPCR) is an indispensable tool in molecular research, which requires reference gene validation to obtain consistent and reliable results.

Objective: To determine the stability of candidate reference genes for qPCR in the joint of collagen-induced arthritis (CIA) and spontaneous arthritis (SpAD) DBA/1 mice. Read More

View Article and Full-Text PDF

Impact of electric cardioversion on platelet activation.

PLoS One 2021 22;16(4):e0250353. Epub 2021 Apr 22.

Division of General Paediatrics, Department of Paediatrics and Adolescent Medicine, Medical University of Graz, Graz, Austria.

Introduction: Atrial fibrillation (AF) comes along with high risk of stroke. This risk continues even after re-establishing sinus rhythm with cardioversion. Aim of this study is to evaluate the contribution of electric cardioversion (EC) to platelet activation and procoagulatory tendency. Read More

View Article and Full-Text PDF

Collagen quantification in the ventricular walls of the heart of the common marmoset (Callithrix jacchus).

Anat Rec (Hoboken) 2021 Apr 19. Epub 2021 Apr 19.

Anatomy of the Domestic and Wild Animals Sector of Universidade de São Paulo (USP), São Paulo, Brazil.

The excellent adaptability of Callithrix jacchus to life in captivity presents advantages in comparison to other nonhuman primates that are used in experimental models for biomedical research, which explains the increasing scientific interest in investigating the anatomical characteristics of this species. Owing to the relative scarcity of publications on the descriptive morphology of the heart of C. jacchus, the aim of this study was to quantify the presence of collagen in the left and right ventricular myocardium using modified picrosirius red and acid fuchsine colorimetric assays. Read More

View Article and Full-Text PDF

Photobiomodulation Therapy on Myocardial Infarction in Rats: Transcriptional and Posttranscriptional Implications to Cardiac Remodeling.

Lasers Surg Med 2021 Apr 13. Epub 2021 Apr 13.

Universidade Nove de Julho, Rua Vergueiro 249, Liberdade, São Paulo, SP, 01504-001, Brazil.

Background And Objectives: Induction of myocardial infarction (MI) in rats by occlusion of the left anterior descending coronary artery is an experimental model used in research to elucidate functional, structural, and molecular modifications associated with ischemic heart disease. Photobiomodulation therapy (PBMT) has become a therapeutic alternative by modulating various biological processes eliciting several effects, including anti-inflammatory and pro-proliferative actions. The main objective of this work was to evaluate the effect of PBMT in the modulation of transcriptional and post-transcriptional changes that occurred in myocardium signal transduction pathways after MI. Read More

View Article and Full-Text PDF

Label-Free Characterization of Collagen Crosslinking in Bone-Engineered Materials Using Nonlinear Optical Microscopy.

Microsc Microanal 2021 Apr 8:1-11. Epub 2021 Apr 8.

Integrative Stem Cell Center, China Medical University Hospital, No. 2, Yude Road, Taichung40447, Taiwan R.O.C.

Engineered biomaterials provide unique functions to overcome the bottlenecks seen in biomedicine. Hence, a technique for rapid and routine tests of collagen is required, in which the test items commonly include molecular weight, crosslinking degree, purity, and sterilization induced structural change. Among them, the crosslinking degree mainly influences collagen properties. Read More

View Article and Full-Text PDF

Carboxymethyl chitosan hydrogel formulations enhance the healing process in experimental partial-thickness (second-degree) burn wound healing.

Acta Cir Bras 2021 5;36(3):e360303. Epub 2021 Apr 5.

PhD. Universidade Federal de Goiás - Instituto de Patologia Tropical e Saúde Pública - Departamento de Biociências e Tecnologia - Goiânia (GO), Brazil.

Purpose: This study aimed to elaborate a hydrogel constituted by carboxymethyl chitosan (CMC), hyaluronic acid (HA) and silver (Ag) and to evaluate its healing effect on partial-thickness burn wounds experimentally induced in rats.

Methods: CMC was obtained by chitosan reacting with monochloroacetic acid. The carboxymethylation was confirmed by Fourier-transform infrared spectroscopy and hydrogen nuclear magnetic resonance (NMR). Read More

View Article and Full-Text PDF

Tissue-specific biomarkers in gingival crevicular fluid are correlated with external root resorption caused by constant mechanical load: an in vivo study.

Clin Oral Investig 2021 Apr 6. Epub 2021 Apr 6.

Department of Orthodontics, The Institute of Craniofacial Deformity, College of Dentistry, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Korea.

Objectives: This study investigated the association of changes in cementum protein-1 (CEMP-1), dentine phosphoprotein (DPP), and c-terminal cross-linked telopeptide of type I collagen (CTX-I) levels in human gingival crevicular fluid (GCF) under constant load with external root resorption volume and amount of tooth movement.

Materials And Methods: In total, 11 healthy adult patients (mean age, 23.5 years [range, 18. Read More

View Article and Full-Text PDF

Prospective, Randomized, Comparative Study of the Cutaneous Effects of a Topical Body Treatment Compared to a Bland Moisturizer.

Aesthet Surg J 2021 Apr 2. Epub 2021 Apr 2.

Alastin Skin Care Inc., Vancouver, BC, Canada.

Background: Over time human skin thins and loses elasticity, and topical treatments attempt to reverse this process.

Objectives: Assess the efficacy of TransFORM Body Treatment (TFB) in skin rejuvenation compared to a bland moisturizer on the extensor and volar forearms.

Methods: Blinded participants were given two products to apply on the designated forearms with follow-up at 4, 8 and 12 weeks. Read More

View Article and Full-Text PDF

Regional Mechanical Characterization of Porcine Pulmonary Arteries.

Exp Mech 2021 Jan 7;61(1):285-303. Epub 2021 Jan 7.

University of Texas at San Antonio, Department of Mechanical Engineering, San Antonio, TX.

Background: Regional mechanical characterization of pulmonary arteries can be useful in the development of computational models of pulmonary arterial mechanics.

Objective: We performed a biomechanical and microstructural characterization study of porcine pulmonary arteries, inclusive of the main, left, and right pulmonary arteries (MPA, LPA, and RPA, respectively).

Methods: The specimens were initially stored at -20°C and allowed to thaw for 12-24 hours prior to testing. Read More

View Article and Full-Text PDF
January 2021

Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV).

J Funct Biomater 2021 Mar 9;12(1). Epub 2021 Mar 9.

Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH, UK.

In addition to biocompatibility, an ideal scaffold for the regeneration of valvular tissue should also replicate the natural heart valve extracellular matrix (ECM) in terms of biomechanical properties and structural stability. In our previous paper, we demonstrated the development of collagen type I and hyaluronic acid (HA)-based scaffolds with interlaced microstructure. Such hybrid scaffolds were found to be compatible with cardiosphere-derived cells (CDCs) to potentially regenerate the diseased aortic heart valve. Read More

View Article and Full-Text PDF

GAKTpore: Stereological Characterisation Methods for Porous Foams in Biomedical Applications.

Materials (Basel) 2021 Mar 7;14(5). Epub 2021 Mar 7.

School of Engineering, University of Leicester, Leicester LE1 7RH, UK.

In tissue engineering, scaffolds are a key component that possess a highly elaborate pore structure. Careful characterisation of such porous structures enables the prediction of a variety of large-scale biological responses. In this work, a rapid, efficient, and accurate methodology for 2D bulk porous structure analysis is proposed. Read More

View Article and Full-Text PDF

Second-harmonic generation (SHG) microscopy and hepatic venous pressure gradient-based validation of a novel histological staging system for alcoholic hepatitis.

Virchows Arch 2021 Apr 2. Epub 2021 Apr 2.

Institute of Liver & Biliary Sciences, D-1 Vasant Kunj, Delhi, 110070, India.

Alcoholic hepatitis (AH) lacks specific histological staging. A novel fibrosis staging that encompasses perisinusoidal fibrosis and cirrhosis sub-stages, substantiated by Hepatic venous pressure gradient (HVPG) and automated fibrosis quantification, is imperative. To correlate novel histological staging system of AH with second-harmonic generation microscopy (SHG)-based q-fibrosis, HVPG, and activated hepatic stellate cells (HSCs). Read More

View Article and Full-Text PDF

Quantifying Breast Cancer-Driven Fiber Alignment and Collagen Deposition in Primary Human Breast Tissue.

Front Bioeng Biotechnol 2021 15;9:618448. Epub 2021 Mar 15.

Department of Surgery, Louisiana State University Health Sciences Center New Orleans, New Orleans, LA, United States.

Solid tumor progression is significantly influenced by interactions between cancer cells and the surrounding extracellular matrix (ECM). Specifically, the cancer cell-driven changes to ECM fiber alignment and collagen deposition impact tumor growth and metastasis. Current methods of quantifying these processes are incomplete, require simple or artificial matrixes, rely on uncommon imaging techniques, preclude the use of biological and technical replicates, require destruction of the tissue, or are prone to segmentation errors. Read More

View Article and Full-Text PDF

Pregnancy-Related Change in pQCT and Bone Biochemistry in a Population With a Habitually Low Calcium Intake.

J Bone Miner Res 2021 Mar 30. Epub 2021 Mar 30.

MRC Nutrition and Bone Health Research Group, University of Cambridge, Cambridge, UK.

In pregnancy, changes in maternal calcium (Ca) economy occur to satisfy fetal Ca demand. It is unclear whether maternal mineral reserves facilitate these requirements and no data exist from sub-Saharan Africa. The aim was to determine skeletal changes with peripheral quantitative computed tomography (pQCT) and bone biochemistry between early second and third trimesters. Read More

View Article and Full-Text PDF

Electrotherapy for urethral modulation: Are extracellular matrix molecules and growth factors potential targets?

Neurourol Urodyn 2021 Mar 26. Epub 2021 Mar 26.

Department of Gynecology, Universidade Federal de São Paulo, São Paulo, Brazil.

Aims: To evaluate the expression of genes and proteins involved in the urethral components: vessels, nerves, and extracellular matrix, in female rats after trauma by vaginal distension (VD) and after electrical stimulation therapy (electrotherapy).

Methods: We analyzed the urethras of three groups of 18 female rats 30 days posttrauma by VD: control (no interventions); trauma (animals that had VD); and electrotherapy group (those that had VD and were treated with electrical stimulation). We compared the expression of vascular endothelial growth factor (VEGF), nerve growth factor (NGF), collagen types I and III (COL1a1 and COL3a1), and lysyl-oxidase like 1 (LOXL1) among the groups. Read More

View Article and Full-Text PDF

Microscope-Based Automated Quantification of Liver Fibrosis in Mice Using a Deep Learning Algorithm.

Toxicol Pathol 2021 Mar 26:1926233211003866. Epub 2021 Mar 26.

Consultant in Toxicologic Pathology, 26745Tel Aviv and Tel Aviv University, Israel.

In preclinical studies that involve animal models for hepatic fibrosis, accurate quantification of the fibrosis is of utmost importance. The use of digital image analysis based on deep learning artificial intelligence (AI) algorithms can facilitate accurate evaluation of liver fibrosis in these models. In the present study, we compared the quantitative evaluation of collagen proportionate area in the carbon tetrachloride model of liver fibrosis in the mouse by a newly developed AI algorithm to the semiquantitative assessment of liver fibrosis performed by a board-certified toxicologic pathologist. Read More

View Article and Full-Text PDF

Detection and Quantification of Myocardial Fibrosis Using Stain-Free Infrared Spectroscopic Imaging.

Arch Pathol Lab Med 2021 Mar 23. Epub 2021 Mar 23.

Department of Chemical and Biomolecular Engineering (Falahkheirkhah, Bhargava).

Context.—: Myocardial fibrosis underpins a number of cardiovascular conditions and is difficult to identify with standard histologic techniques. Challenges include imaging, defining an objective threshold for classifying fibrosis as mild or severe, as well as understanding the molecular basis for these changes. Read More

View Article and Full-Text PDF

Three-Dimensional Quantification of Collagen Microstructure During Tensile Mechanical Loading of Skin.

Front Bioeng Biotechnol 2021 3;9:642866. Epub 2021 Mar 3.

Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, United States.

Skin is a heterogeneous tissue that can undergo substantial structural and functional changes with age, disease, or following injury. Understanding how these changes impact the mechanical properties of skin requires three-dimensional (3D) quantification of the tissue microstructure and its kinematics. The goal of this study was to quantify these structure-function relationships second harmonic generation (SHG) microscopy of mouse skin under tensile mechanical loading. Read More

View Article and Full-Text PDF

Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals.

Zoo Biol 2021 Mar 19. Epub 2021 Mar 19.

Laboratory of Animal Biotechnology, Center for Biological and Health Sciences, Federal Rural University of Semi-Arid, Mossoro, Rio Grande do Norte, Brazil.

Biological resource banks represent valuable tools for the conservation of species vulnerable to extinction, such as the jaguar. Cryobanks of skins have the potential to safeguard rare genotypes, allowing the potential exploitation of biological samples in animal multiplication technologies and the study of genetic variability. Determination of the most suitable skin regions for tissue conservation can help increase the efficiency of cryobanks and the storage of biological samples. Read More

View Article and Full-Text PDF

Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples.

PLoS One 2021 18;16(3):e0248068. Epub 2021 Mar 18.

Infraredx, Bedford, Massachusetts, United States of America.

Collagen is an important component in maintaining structural integrity and functionality of tissues and is modulated in various biological processes. Its visualization and possible quantification using histopathological stains can be important for understanding disease progression or therapeutic response. Visualization of collagen fiber with the histological stain picrosirius red (PSR) is enhanced with polarized light and quantitative analysis is possible using circular polarizers. Read More

View Article and Full-Text PDF

Quantitative, noninvasive MRI characterization of disease progression in a mouse model of non-alcoholic steatohepatitis.

Sci Rep 2021 Mar 17;11(1):6105. Epub 2021 Mar 17.

Department of Radiology, Massachusetts General Hospital, Athinoula A. Martinos Center for Biomedical Imaging, Institute for Innovation in Imaging, Harvard Medical School, 149 13th St., Boston, MA, 02129, USA.

Non-alcoholic steatohepatitis (NASH) is an increasing cause of chronic liver disease characterized by steatosis, inflammation, and fibrosis which can lead to cirrhosis, hepatocellular carcinoma, and mortality. Quantitative, noninvasive methods for characterizing the pathophysiology of NASH at both the preclinical and clinical level are sorely needed. We report here a multiparametric magnetic resonance imaging (MRI) protocol with the fibrogenesis probe Gd-Hyd to characterize fibrotic disease activity and steatosis in a common mouse model of NASH. Read More

View Article and Full-Text PDF

Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE mice.

Sci Rep 2021 Mar 12;11(1):5834. Epub 2021 Mar 12.

Department of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstraße 76, 01307, Dresden, Germany.

Aortic valve sclerosis is characterized as the thickening of the aortic valve without obstruction of the left ventricular outflow. It has a prevalence of 30% in people over 65 years old. Aortic valve sclerosis represents a cardiovascular risk marker because it may progress to moderate or severe aortic valve stenosis. Read More

View Article and Full-Text PDF

Evaluating Melanoma Viability and Proliferation in 3D Microenvironments.

Methods Mol Biol 2021 ;2265:155-171

Lyda Hill Department of Bioinformatics and Department of Cell Biolog, UT Southwestern Medical Center, Dallas, TX, USA.

Researchers often aim to incorporate microenvironmental variables such as the dimensionality and composition of the extracellular matrix into their cell-based assays. A technical challenge created by introduction of these variables is quantification of single-cell measurements and control of environmental reproducibility. Here, we detail a methodology to quantify viability and proliferation of melanoma cells in 3D collagen-based culture platforms by automated microscopy and 3D image analysis to yield robust, high-throughput results of single-cell responses to drug treatment. Read More

View Article and Full-Text PDF

Rapid Evaluation of Novel Therapeutic Strategies Using a 3D Collagen-Based Tissue-Like Model.

Front Bioeng Biotechnol 2021 16;9:574035. Epub 2021 Feb 16.

Université Paris-Saclay, CNRS, Institut des Sciences Moléculaires d'Orsay, Orsay, France.

2D cell cultures are commonly used to rapidly evaluate the therapeutic potential of various treatments on living cells. However, the effects of the extracellular matrix (ECM) including the 3D arrangement of cells and the complex physiology of native environment are missing, which makes these models far from conditions. 3D cell models have emerged in preclinical studies to simulate the impact of the ECM and partially bridge the gap between monolayer cultures and tissues. Read More

View Article and Full-Text PDF
February 2021

Inhibition of hypertrophic scar formation with oral asiaticoside treatment in a rabbit ear scar model.

Int Wound J 2021 Mar 5. Epub 2021 Mar 5.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Hypertrophic scar (HS) is a fibrotic skin disease characterised by over-productive collagen and excessive inflammatory reaction, which can be functionally and cosmetically problematic. A scar-prone constitute will accelerate HS formation and functional disorder, which deserves systemic therapy with oral medicine. To examine the oral therapeutic effectiveness on HS with convincing evidence of gross view and histological improvement, a rabbit ear HS model was employed with oral administration of asiaticoside (AS) at the doses of 12 and 24 mg kg  d daily for 60 consecutive days. Read More

View Article and Full-Text PDF

Microphysiological System for High-Throughput Computer Vision Measurement of Microtissue Contraction.

ACS Sens 2021 03 3;6(3):985-994. Epub 2021 Mar 3.

Joint Department of Biomedical Engineering, University of North Carolina-Chapel Hill/North Carolina State University, Raleigh 27695, North Carolina, United States.

The ability to measure microtissue contraction in vitro can provide important information when modeling cardiac, cardiovascular, respiratory, digestive, dermal, and skeletal tissues. However, measuring tissue contraction in vitro often requires the use of high number of cells per tissue construct along with time-consuming microscopy and image analysis. Here, we present an inexpensive, versatile, high-throughput platform to measure microtissue contraction in a 96-well plate configuration using one-step batch imaging. Read More

View Article and Full-Text PDF

A novel apoptosis probe, cyclic ApoPep-1, for in vivo imaging with multimodal applications in chronic inflammatory arthritis.

Apoptosis 2021 Apr 3;26(3-4):209-218. Epub 2021 Mar 3.

Department of Internal Medicine (Rheumatology), Kyungpook National University School of Medicine, 680 Gukchaebosang-ro, Junggu, Daegu, 41944, Republic of Korea.

Apoptosis plays an essential role in the pathophysiologic processes of rheumatoid arthritis. A molecular probe that allows spatiotemporal observation of apoptosis in vitro, in vivo, and ex vivo concomitantly would be useful to monitoring or predicting pathophysiologic stages. In this study we investigated whether cyclic apoptosis-targeting peptide-1 (ApoPep-1) can be used as an apoptosis imaging probe in inflammatory arthritis. Read More

View Article and Full-Text PDF

AGEs accumulation is related to muscle degeneration and vascular calcification in peritoneal dialysis patients.

J Bras Nefrol 2021 Feb 26. Epub 2021 Feb 26.

Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Laboratório para o Estudo do Distúrbio Mineral e Ósseo em Nefrologia, Campinas, SP, Brasil.

Background: Patients with chronic kidney disease (CKD) are affected by dynapenia, sarcopenia, and vascular calcification. Advanced glycation end products (AGEs) may accumulate in peritoneal dialysis (PD) patients and favor sarcopenia via changes in collagen cross-linking, muscle protein breakdown, and the calcification of arterial smooth muscle cells via p38-MAPK activation. The aim of this study is to explore the relationships between AGEs, muscle degeneration, and coronary artery calcification. Read More

View Article and Full-Text PDF
February 2021