8,624 results match your criteria quality sperms

Structure-based redesigning of pentoxifylline analogs against selective phosphodiesterases to modulate sperm functional competence for assisted reproductive technologies.

Sci Rep 2021 Jun 10;11(1):12293. Epub 2021 Jun 10.

Macromolecular Structural Biology Lab, Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Telangana, 502285, India.

Phosphodiesterase (PDE) inhibitors, such as pentoxifylline (PTX), are used as pharmacological agents to enhance sperm motility in assisted reproductive technology (ART), mainly to aid the selection of viable sperm in asthenozoospermic ejaculates and testicular spermatozoa, prior to intracytoplasmic sperm injection (ICSI). However, PTX is reported to induce premature acrosome reaction (AR) and, exert toxic effects on oocyte function and early embryo development. Additionally, in vitro binding studies as well as computational binding free energy (ΔG) suggest that PTX exhibits weak binding to sperm PDEs, indicating room for improvement. Read More

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Differentially expressed microRNAs in biochemically characterized Frieswal crossbred bull semen.

Anim Biotechnol 2021 Jun 9:1-14. Epub 2021 Jun 9.

Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, India.

In addition to the transmission of paternal genome, spermatozoa also carry coding as well as noncoding microRNAs (miRNAs) into the female oocyte during the process of biological fertilization. Based on RNA deep sequencing, a total 28 number of differentially expressed miRNAs were cataloged in categorized Frieswal crossbred (Holstein Friesian X Sahiwal) bull semen on the basis of conception rate (CR) in field progeny testing program. Validation of selected miRNAs viz. Read More

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Progressive bovine sperm separation using parallelized microchamber-based microfluidics.

Lab Chip 2021 Jun 9. Epub 2021 Jun 9.

Food Science Department, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA.

Motility is one of the most important factors in sperm migration toward an egg. Therefore, sperm separation based on motility might enhance sperm selection for infertility treatments. Conventional centrifugation-based methods increase the risk of damage to sperm cells. Read More

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Nuclear heterogeneity is prevalent in high-quality fractionated human sperm cells typically used for assisted conception.

Hum Reprod 2021 Jun 7. Epub 2021 Jun 7.

Department of Biological Science, University of Newcastle, Callaghan, NSW, Australia.

Study Question: What is the nuclear heterogeneity of high-density purified human spermatozoa typically used for IVF purposes.

Summary Answer: The data show that while density gradient separation has improved the overall sperm population, there is still a large degree of nuclear heterogeneity within these cells.

What Is Known Already: Chromomycin A3 (CMA3) is an important DNA binding fluorochrome for the assessment of male-factor fertility. Read More

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Cumulative IVF outcomes after retrieval of testicular spermatozoa: should we use immotile spermatozoa for ICSI?

Reprod Biomed Online 2021 Mar 17. Epub 2021 Mar 17.

Department of Obstetrics and Gynecology, Chaim Sheba Medical Center (Tel Hashomer), Ramat Gan 5621, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; The Tarnesby-Tarnowski Chair for Family Planning and Fertility Regulation, at the Sackler Faculty of Medicine, Tel-Aviv University, Israel.

Research Question: What are the cumulative clinical pregnancy rates (CPR) and live births rates (LBR) in intracytoplasmic sperm injection (ICSI) cycles using testicular motile compared with immotile spermatozoa, obtained from testicular sperm aspiration (TESA) or extraction (TESE)?

Design: A retrospective analysis of ICSI cycles using TESA or TESE over a period of 7 years. Cycles were divided into two groups according to the motility of the retrieved spermatozoa: Group A consisted of couples with motile spermatozoa; Group B of couples with immotile spermatozoa. Group B was subdivided into two groups: B1 consisted of couples with motile spermatozoa and B2 with immotile spermatozoa after the addition of pentoxifylline. Read More

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Patients with acephalic spermatozoa syndrome linked to novel TSGA10/PMFBP1 variants have favorable pregnancy outcomes from ICSI.

Clin Genet 2021 Jun 4. Epub 2021 Jun 4.

Reproductive and Genetic Hospital of CITIC-Xiangya, China.

Acephalic spermatozoa syndrome is a rare form of teratozoospermia characterized by headless spermatozoa.Previous studies have found that variants in SUN5, PMFBP1, TSGA10, BRDT, and SPATC1L are associated with this phenotype.Many researchers have suggested that variants in TSGA10 without a proximal centriole might influence early embryonic development. Read More

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Optimization of small RNA extraction and comparative study of NGS library preparation from low count sperm samples.

Syst Biol Reprod Med 2021 Jun 3;67(3):230-243. Epub 2021 Jun 3.

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.

Recent studies demonstrate that sperm epigenome is a vehicle that conveys paternal experiences to offspring phenotype. That evidence triggers interest of both experimental and epidemiological studies of epigenetic markers in sperm. Since samples are often unique in epidemiological studies, a careful and efficient use of the material is a critical requirement. Read More

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Factors affecting rabbit sperm cryopreservation: a mini-review.

Zygote 2021 Jun 3:1-8. Epub 2021 Jun 3.

National Agricultural and Food Centre (NPPC), Research Institute for Animal Production, Nitra, Slovak Republic.

Rabbits are an important animal species for meeting the nutritional requirements of the world's growing population due to the high conversion rate of feed. In most countries, the rabbit industry currently relies on artificial insemination with fresh or chilled and frozen-thawed spermatozoa. Various factors during the freezing process, including diluents, sperm preparation and freezing techniques, antioxidants, sudden temperature changes, ice formation and osmotic stress, have been proposed as reasons for the poor sperm quality post thaw. Read More

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Detailed cell-level analysis of sperm nuclear quality among the different hypo-osmotic swelling test (HOST) classes.

J Assist Reprod Genet 2021 Jun 2. Epub 2021 Jun 2.

Département de Génétique Médicale, Sorbonne Université, INSERM, Maladies génétiques d'expression pédiatrique, APHP, Hôpital d'Enfants Armand Trousseau, 26 avenue du Dr Arnold Netter, F-75012, Paris, France.

Purpose: We studied the quality differences between the different hypo-osmotic swelling test (HOST) classes, as measured by criteria of DNA fragmentation, DNA decondensation, and nuclear architecture. The aim was to find particular HOST classes associated with good-quality metrics, which may be potentially used in ICSI (intra-cytoplasmic sperm injection).

Methods: Ten patients from the Department of Reproductive Medicine at Tenon Hospital (Paris, France) were included. Read More

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Osmoregulation in fish sperm.

Fish Physiol Biochem 2021 Jun 2. Epub 2021 Jun 2.

Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.

In most fish exhibiting external fertilization, spermatozoa become motile after release into water, triggered by differences between intracellular and extracellular conditions such as osmotic pressure, ion composition, and pH. The rapid change in osmolarity initiating spermatozoon motility induces osmotic pressure, resulting in active water movement across the cell membrane. Mechanisms of ion and water transport across the plasma membrane and cell volume regulation are important in maintaining structure and functional integrity of the cell. Read More

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Effect of different cryopreservation extenders added with antioxidants on semen quality and in vitro embryo production efficiency in cattle.

An Acad Bras Cienc 2021 31;93(3):e20191229. Epub 2021 May 31.

Universidade de Brasília, Instituto de Biologia, Campus Universitário Darcy Ribeiro, s/n, Asa Norte, 70910-900 Brasília, DF, Brazil.

To evaluate the addition of antioxidants in extenders on post-thaw bovine semen quality and in vitro embryo production efficiency. Six semen samples were collected from five Holstein bulls. In the experiment I, the samples were diluted with AndroMed® and Bovimix® and added antioxidants glutathione (1. Read More

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Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp .

Int J Mol Sci 2021 May 31;22(11). Epub 2021 May 31.

South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.

The purpose of the current study was to analyze phenotypic and functional characteristics of common carp () spermatozoa during in vitro aging and to investigate whether global DNA methylation is affected by sperm aging. Milt was collected from five individual males, stored in vitro on ice in a refrigerator for up to 96 h post stripping (HPS) and used to fertilize eggs with intervals of 1, 24 and 96 h. Computer-assisted sperm analysis and a S3e Cell Sorter was employed to determine the spermatozoa phenotypic characteristics (motility, velocity, concentration and viability). Read More

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Plant Extracts as Alternative Additives for Sperm Preservation.

Antioxidants (Basel) 2021 May 13;10(5). Epub 2021 May 13.

Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague 6-Suchdol, Czech Republic.

Sperm preservation is a crucial factor for the success of assisted reproductive technology (ART) in humans, livestock, and wildlife. Irrespective of the extender and the storage conditions used, semen handling and preservation negatively affect sperm quality. Moreover, oxidative stress, which often arises during semen storage, significantly reduces sperm function and compromises the sperm fertilizing ability by inducing oxidative damage to proteins, lipids, and nucleic acids. Read More

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The Effect of Non-Thermal Plasma on the Structural and Functional Characteristics of Human Spermatozoa.

Int J Mol Sci 2021 May 7;22(9). Epub 2021 May 7.

Department of Genetics, Faculty of Natural Sciences, Comenius University, Ilkovičova 6, Mlynská dolina, 842 15 Bratislava, Slovakia.

Significant antibacterial properties of non-thermal plasma (NTP) have converted this technology into a promising alternative to the widespread use of antibiotics in assisted reproduction. As substantial data available on the specific in vitro effects of NTP on male reproductive cells are currently missing, this study was designed to investigate selected quality parameters of human spermatozoa (n = 51) exposed to diffuse coplanar surface barrier discharge NTP for 0 s, 15 s, 30 s, 60 s and 90 s. Sperm motility characteristics, membrane integrity, mitochondrial activity, production of reactive oxygen species (ROS), DNA fragmentation and lipid peroxidation (LPO) were investigated immediately following exposure to NTP and 2 h post-NTP treatment. Read More

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Challenges in the development of sperm cryopreservation protocols for snakes.

Reprod Fertil Dev 2021 Jun 1. Epub 2021 Jun 1.

Snake populations are declining worldwide, but research devoted to the development of sperm cryopreservation techniques for this taxon is very limited. Spermatozoa were collected postmortem from snakes of four squamate families (Elapidae, Colubridae, Viperidae and Pythonidae). Viability assessment was performed before and after cryopreservation. Read More

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Sperm migration, selection, survival and fertilizing ability in the mammalian oviduct.

Biol Reprod 2021 May 31. Epub 2021 May 31.

CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France.

In vitro fertilization (IVF) gives rise to embryos in a number of mammalian species and is currently widely used for assisted reproduction in humans and for genetic purposes in cattle. However, the rate of polyspermy is generally higher in vitro than in vivo and IVF remains ineffective in some domestic species like pigs and horses, highlighting the importance of the female reproductive tract for gamete quality and fertilization. In this review, the way the female environment modulates sperm selective migration, survival and acquisition of fertilizing ability in the oviduct is being considered under six aspects: (1) the utero-tubal junction which selects a sperm sub-population entering the oviduct; (2) the presence of sperm binding sites on luminal epithelial cells in the oviduct, which prolong sperm viability and plays a role in limiting polyspermic fertilization; (3) the contractions of the oviduct, which promote sperm migration toward the site of fertilization in the ampulla; (4) the regions of the oviduct, which play different roles in regulating sperm physiology and interactions with oviduct epithelial cells; (5) the time of ovulation and (6) the steroid hormonal environment which regulates sperm release from the luminal epithelial cells and facilitates capacitation in a finely orchestrated manner. Read More

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Is addition or removal of seminal plasma able to compensate for the dilution effect of buffalo semen?

Andrologia 2021 May 30:e14123. Epub 2021 May 30.

Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, India.

The present study aimed to compensate dilution effect using additional seminal plasma (SP) in conventional (80 million (M) spermatozoa/ml) dose and low spermatozoa/dose (8M spermatozoa/ml). We also attempted to confirm whether removal of SP before the extension of ejaculates affects post-thaw sperm quality of buffalo semen. For this, semen ejaculates (N = 15) were divided into four groups: control (CON), removal of SP by centrifugation (NSP), resuspension of the centrifuged semen pellet into SP (CEN) and extra supplementation of SP (ESP). Read More

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Semen quality as a potential susceptibility indicator to SARS-CoV-2 insults in polluted areas.

Environ Sci Pollut Res Int 2021 May 29. Epub 2021 May 29.

Department of Biology, University of Naples Federico II, Via Cinthia 21, 80126, Napoli, Italy.

The epidemic of the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted worldwide with its infectious spread and mortality rate. Thousands of articles have been published to tackle this crisis and many of these have indicated that high air pollution levels may be a contributing factor to high outbreak rates of COVID-19. Atmospheric pollutants, indeed, producing oxidative stress, inflammation, immuno-unbalance, and systemic coagulation, may be a possible significant co-factor of further damage, rendering the body prone to infections by a variety of pathogens, including viruses. Read More

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Amelioration of sperm fertilizability, thyroid activity, oxidative stress, and inflammatory cytokines in rabbit bucks treated with phytogenic extracts.

Anim Sci J 2021 Jan;92(1):e13560

Department of Poultry Production, Faculty of Agriculture, Damietta University, Damietta, Egypt.

This study investigated the beneficial effect of phytogenic extracts on semen quality, reproductive hormones, thyroid activity, immunity, hepatic antioxidant activity, and fertility in rabbit bucks. We divided 70 bucks into seven groups (10 in each). Group 1 was fed a basal diet (control); groups 2, 3, and 4 were fed the control diet with 30, 60, and 90 mg/kg of turmeric, respectively; and groups 5, 6, and 7 were fed the control diet with 50, 75, and 100 mg/kg of garlic extract, respectively, for 8 weeks. Read More

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January 2021

Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine.

Front Vet Sci 2021 7;8:650572. Epub 2021 May 7.

Grupo BIOFITER-Departamento de Bioquímica y Biología Molecular y Celular-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.

The prediction of the fertilizing ability of a seminal dose continues to be a primary aim in the field of artificial insemination (AI). To achieve this goal, in this study we have included the evaluation of some non-conventional sperm quality markers. A total of 3,906 ewes from 52 different farms were inseminated with 357 refrigerated seminal doses obtained from 45 mature rams. Read More

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Evaluation of the standards compliance of the queen bees reared in the Mediterranean region in Turkey.

Saudi J Biol Sci 2021 May 12;28(5):2686-2691. Epub 2021 Mar 12.

Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, Taif 21944, Saudi Arabia.

The influence of different commercial queen producers on the quality of queens was assessed. It was aimed to determine the quality characteristics of queens reared by commercial queen producers located in the province of Antalya, which is an important region in queens production due to its climatic characteristics. For this purpose, the quality characteristics of a total of 105 queen bees obtained from 21 enterprises were determined. Read More

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The effect of lipopolysaccharide from uropathogenic Escherichia coli on the immune system, testis tissue, and spermatozoa of BALB/c mice.

Clin Exp Reprod Med 2021 Jun 21;48(2):105-110. Epub 2021 May 21.

Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Objective: Uropathogenic Escherichia coli is known to cause urinary tract infections, and the endotoxin (lipopolysaccharide [LPS]) of this bacterium may cause deficiencies of sperm quality and morphology. In the present study, the effects of LPS on mouse sperm were studied, and the levels of interleukin (IL)-17A and possible changes in testis tissue were evaluated.

Methods: LPS of uropathogenic E. Read More

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Single-blind clinical trial: Sperm selection based on capacity to pass through cumulus oophorous column improves ICSI outcomes.

Andrology 2021 May 21. Epub 2021 May 21.

Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

Background: Sperm selection procedures for future strategies that aim to select normal spermatozoa with intact DNA to improve intracytoplasmic sperm injection (ICSI) outcomes are in early developing stage.

Objectives: The objective is to find out whether the sperm selection procedure based on the ability of spermatozoa to traverse the cumulus cells could improve clinical outcomes of ICSI technique in infertile couples with male factor etiology.

Materials And Methods: For this single-blind clinical trial, mature metaphase II oocytes were retrieved from 150 couples with male factor infertility, male age lower than 45 years and female age under 38 years. Read More

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Organophosphorus pesticide quinalphos (Ekalux 25 E.C.) reduces sperm functional competence and decreases the fertilisation potential in Swiss albino mice.

Andrologia 2021 May 20:e14115. Epub 2021 May 20.

Department of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India.

Quinalphos (QP) is one of the most commonly used organophosphate pesticide for agriculture. In this study, adult Swiss albino male mice were orally administered with 0.25, 0. Read More

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Gene Expression Alteration of Sperm-Associated Antigens in Human Cryopreserved Sperm.

Biopreserv Biobank 2021 May 18. Epub 2021 May 18.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Sperm-associated antigens (SPAGs) are 18 types of proteins, some of which play important roles in various biological functions associated with assisted reproductive technology outcomes, and are consequently important to the success of fertility programs. Despite the favorable outcomes of fecundity rates among male patients with cancer using cryopreserved sperm, the detrimental impact of freezing on cells has been noted in many studies. Cryopreservation has been thought to have adverse effects on sperm quality through disruptions in the expressions of genes. Read More

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Trehalose modifies the protein profile of ram spermatozoa during cryopreservation.

Theriogenology 2021 Sep 6;171:21-29. Epub 2021 May 6.

Yunnan Animal Science and Veterinary Institute, Jindian, Panlong County, Kunming City, Yunnan province, China; Yunnan Provincial Engineering Laboratory of Animal Genetic Resource Conservation and Germplasm Enhancement, Jindian, Panlong County, Kunming City, Yunnan province, China. Electronic address:

As a magical oligosaccharide, trehalose has been revealed to enhance the post-thaw quality of stock semen. However, information regarding the cryoprotective mechanism of trehalose during cryopreservation has not yet been determined. This study was designed to observe the effects of trehalose on the proteome of ram frozen spermatozoa by applying the isobaric tag for relative and absolute quantification (iTRAQ) strategy combined with parallel reaction monitoring (PRM). Read More

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September 2021

The Effects of Folic Acid on Testicular Histology, Sperm Quality, and Spermatogenesis Indices Following 3,4-Methylenedioxymethamphetamine Exposure in Adult Male Rats.

Addict Health 2021 Jan;13(1):36-44

Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Background: There is an increasing concern over acute exposure of amphetamine and its derivative such as 3,4-methylenedioxymethamphetamine (MDMA) on male reproductive toxicity. Supplementary vitamins can reduce the oxidative stresses and repair the damages on reproductive organs. This experimental study was conducted to evaluate the effects of folic acid (FA) on reproductive indices, the antioxidant enzyme activities, and histological changes of testis on adult male rats treated by MDMA. Read More

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January 2021

L-Proline: An Effective Agent for Frozen and Post-thawed Donkey Semen Storage.

J Equine Vet Sci 2021 Jun 3;101:103393. Epub 2021 Feb 3.

National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address:

The aim of this study was to evaluate the effects of L-proline on the extender quality of frozen and post-thawed jackass semen. Jackass (n = 6) semen samples were collected and cryopreserved in gradient concentrations (0-80 mM) of L-proline in extenders; post-thawed semen samples were cultured in L-proline medium for 10 hours at 37°C. For cryopreservation experiment I, the motile parameters, mitochondrial membrane potential (MMP), and plasma membrane, acrosome, and chromatin structure integrities of post-thawed semen were assessed. Read More

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Incubation with Cholesterol-loaded Cyclodextrin and Subsequent Dilution in Partially Deoxygenated Extender Improves the Freezability of Cross-bred Bull Sperm.

Cryo Letters 2020 Sep-Oct;41(5):257-266

Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.

Background: The cryopreservation process induces osmotic stress, membrane changes and production of reactive oxygen species resulting in damage to the spermatozoa. Together, the presence of oxygen in the extender aggravates the oxidative stress that further reduces the cryosurvival rate of sperm cells.

Objective: To study the combined effect of cholesterol loaded cyclodextrin (CLC) and partial deoxygenation on post-thaw semen quality in crossbred bulls. Read More

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Possible Protective Mechanisms of Coenzyme Q10 Action on Spermatozoa During Cryopreservation or Cooled-Stored Condition.

Cryo Letters 2020 Sep-Oct;41(5):246-256

Joint Laboratory of Modern Agricultural Technology International Cooperation; Key Laboratory of Animal Production, Product Quality, and Security; College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.

Artificial insemination (AI) with frozen or cooled-stored semen plays a key role in the widespread distribution of germplasm of elite livestock resources and the protection of endangered species. Cryopreservation provides long-term preservation of sperm and also encourages a greater exchange of genetic material between distant populations. However, freezing has some detrimental effects on sperm, including premature induction of acrosome response, reduced sperm motility, reduced viability, and impaired sperm DNA integrity and fertility. Read More

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