17 results match your criteria q30 score

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Comparative genome characterization of Leptospira interrogans from mild and severe leptospirosis patients.

Genomics Inform 2021 Sep 30;19(3):e31. Epub 2021 Sep 30.

Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Leptospirosis is a zoonotic disease caused by spirochetes from the genus Leptospira. In Thailand, Leptospira interrogans is a major cause of leptospirosis. Leptospirosis patients present with a wide range of clinical manifestations from asymptomatic, mild infections to severe illness involving organ failure. Read More

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September 2021

BGISEQ-500RS sequencing of a 448-plex SNP panel for forensic individual identification and kinship analysis.

Forensic Sci Int Genet 2021 Aug 20;55:102580. Epub 2021 Aug 20.

National Engineering Laboratory for Forensic Science, Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. Electronic address:

Next generation sequencing (NGS)-based single nucleotide polymorphism (SNP) genotyping is widely used in the field of forensics. SNP genotyping data from several NGS platforms have been published, but forensic application trials of DNA nanoball sequencing platforms have been very limited. In this work, we developed a 448-plex SNP panel on the BGISEQ-500RS platform. Read More

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Next Generation Sequencing-Based Germline Panel Testing for Breast and Ovarian Cancers in Pakistan.

Asian Pac J Cancer Prev 2021 Mar 1;22(3):719-724. Epub 2021 Mar 1.

Department of Histopathology, PNS Shifa Hospital, Karachi, Pakistan.

Background: Pathogenic germline mutations in BRCA1/2 constitute the majority of hereditary breast and/or ovarian cancers worldwide. Incidence and mortality rate of breast and ovarian cancers in Pakistani women is high. Thus, to establish the diagnosis for targeted therapy in Pakistan, we conducted Next-generation sequencing-based germline testing for the detection of BRCA1/2 oncogenic variants associated with breast and ovarian cancer subtype. Read More

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Transcriptome and translatome profiles of Streptomyces species in different growth phases.

Sci Data 2020 05 8;7(1):138. Epub 2020 May 8.

Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.

Streptomyces are efficient producers of various bioactive compounds, which are mostly synthesized by their secondary metabolite biosynthetic gene clusters (smBGCs). The smBGCs are tightly controlled by complex regulatory systems at transcriptional and translational levels to effectively utilize precursors that are supplied by primary metabolism. Thus, dynamic changes in gene expression in response to cellular status at both the transcriptional and translational levels should be elucidated to directly reflect protein levels, rapid downstream responses, and cellular energy costs. Read More

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Effect of a neck compression collar on cardiorespiratory and cerebrovascular function in postural orthostatic tachycardia syndrome (POTS).

J Appl Physiol (1985) 2020 04 12;128(4):907-913. Epub 2020 Mar 12.

School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada.

Postural orthostatic tachycardia syndrome (POTS) is accompanied by reduced brain blood flow, autonomic dysfunction, and orthostatic intolerance. We hypothesized that wearing a neck compression collar would attenuate orthostatic symptoms, increase brain blood flow, and influence autonomic reflexes. Ten participants with POTS (9 women, age: 36 ± 10) underwent two trials of supine rest, paced deep breathing (6 breaths/min), Valsalva maneuver (40 mmHg for 15 s), and 70° upright tilt. Read More

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Transcriptome sequencing assisted discovery and computational analysis of novel SNPs associated with flowering in in-bred lines for marker-assisted backcross breeding.

Hortic Res 2019 1;6:120. Epub 2019 Nov 1.

Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeonju, 55365 Republic of Korea.

The sequencing of radish genome aids in the better understanding and tailoring of traits associated with economic importance. In order to accelerate the genomics assisted breeding and genetic selection, transcriptomes of 33 radish inbred lines with diverse traits were sequenced for the development of single nucleotide polymorphic (SNP) markers. The sequence reads ranged from 2,560,543,741 bp to 20,039,688,139 bp with the GC (%) of 47. Read More

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November 2019

Physiological and transcriptome analyses of photosynthesis and chlorophyll metabolism in variegated Citrus (Shiranuhi and Huangguogan) seedlings.

Sci Rep 2019 10 30;9(1):15670. Epub 2019 Oct 30.

College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, China.

Citrus species are among the most economically important fruit crops. Physiological characteristics and molecular mechanisms associated with de-etiolation have been partially revealed. However, little is known about the mechanisms controlling the expression and function of genes associated with photosynthesis and chlorophyll biosynthesis in variegated citrus seedlings. Read More

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October 2019

Exposure-Response Analysis for Efficacy of Daclatasvir, Asunaprevir, and Beclabuvir Combinations in HCV-Infected Patients.

Clin Pharmacol Drug Dev 2019 10 22;8(7):903-913. Epub 2019 Jan 22.

Bristol-Myers Squibb Research and Development, Lawrenceville, NJ, USA.

The combination regimen of daclatasvir, asunaprevir, and beclabuvir (3DAA regimen) was developed as a fixed-dose combination for the treatment of hepatitis C virus (HCV) infection in Japan. The objectives of this analysis were to characterize the relationship between drug exposure and sustained virologic response at posttreatment week 12 (SVR12) in HCV-infected subjects and to evaluate the impact of demographic covariates and clinical factors on the exposure-response (E-R) relationship. The E-R efficacy analysis was performed with data from phase 2 and phase 3 studies in HCV-infected subjects treated with the 3DAA regimen. Read More

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October 2019

Outcomes of open versus laparoscopic surgery in patients with colon cancer.

Eur J Surg Oncol 2018 09 6;44(9):1344-1353. Epub 2018 Jun 6.

Unidad de Investigación, Hospital Galdakao-Usansolo, Galdakao, Bizkaia, Spain; Red de Investigación en Servicios Sanitarios y Enfermedades Crónicas (REDISSEC).

Objective: There is limited information on health service use or patient-reported outcomes when comparing the effectiveness of laparoscopic with that of open surgery. The aim was to compare the effectiveness of laparoscopic with that of open surgery up to 2 years after intervention in patients with colon cancer.

Methods: Prospective cohort study of patients with colon cancer who underwent surgery (laparoscopic or open surgery) between June 2010 and December 2012, at 22 hospitals. Read More

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September 2018

[Lu]-PSMA-617 radionuclide treatment in patients with metastatic castration-resistant prostate cancer (LuPSMA trial): a single-centre, single-arm, phase 2 study.

Lancet Oncol 2018 06 8;19(6):825-833. Epub 2018 May 8.

Department of Medical Oncology, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC, Australia.

Background: Progressive metastatic castration-resistant prostate cancer is a highly lethal disorder and new effective therapeutic agents that improve patient outcomes are urgently needed. Lutetium-177 [Lu]-PSMA-617, a radiolabelled small molecule, binds with high affinity to prostate-specific membrane antigen (PSMA) enabling beta particle therapy targeted to metastatic castration-resistant prostate cancer. We aimed to investigate the safety, efficacy, and effect on quality of life of [Lu]-PSMA-617 in men with metastatic castration-resistant prostate cancer who progressed after standard treatments. Read More

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Transcriptome Analyses of Two Citrus Cultivars (Shiranuhi and Huangguogan) in Seedling Etiolation.

Sci Rep 2017 04 7;7:46245. Epub 2017 Apr 7.

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

Citrus species are among the most important fruit crops. However, gene regulation and signaling pathways related to etiolation in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in two hybrid citrus cultivars-Huangguogan and Shiranuhi, respectively-were investigated. Read More

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Botulinum toxin therapy for neurogenic detrusor hyperactivity versus augmentation enterocystoplasty: impact on the quality of life of patients with SCI.

Spinal Cord 2016 Nov 26;54(11):1031-1035. Epub 2016 Apr 26.

Rehab Centre COS DIVIO, Dijon, France.

Study Design: Cross-sectional study.

Objectives: To compare quality of life (QoL) in patients with spinal cord injury (SCI) who underwent overactive detrusor treatment by botulinum toxin (BT) versus augmentation cystoplasty (AC).

Setting: France. Read More

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November 2016

Bacterial diversity of Murlen National Park located in Indo-Burman Biodiversity hotspot region: A metagenomic approach.

Genom Data 2015 Sep 8;5:25-6. Epub 2015 May 8.

Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India ; Department of Zoology, Mizoram University, Aizawl 796004, Mizoram, India.

Paired end Illumina Mi-Seq sequencing of 16S rRNA gene amplicon was carried out to study the bacterial community in the soil of Murlen National Park located in Indo-Burman Biodiversity hotspot region. Metagenome consisted of 302,416 reads with 151.81 Mb data and G + C content of 56. Read More

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September 2015

Bladder cancer biorepositories in the "-omics" era: integrating quality tissue specimens with comprehensive clinical annotation.

Biopreserv Biobank 2013 Jun 31;11(3):166-72. Epub 2013 May 31.

1 Department of Urology and Surgery, University of Melbourne , Royal Melbourne Hospital, Parkville, Australia .

Advances in genomic platforms have led to the need for well-characterized, high quality biospecimens for research. Bladder cancer, despite being a major epidemiological concern, has been under-represented in genomic programs due to unique challenges in collection of clinical informatics and tissues. Currently no targeted therapy exists for management of the disease. Read More

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Simultaneous and complete genome sequencing of influenza A and B with high coverage by Illumina MiSeq Platform.

J Virol Methods 2013 Nov 12;193(2):394-404. Epub 2013 Jul 12.

Department of Virology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand. Electronic address:

Active global surveillance and characterization of influenza viruses are essential for better preparation against possible pandemic events. Obtaining comprehensive information about the influenza genome can improve our understanding of the evolution of influenza viruses and emergence of new strains, and improve the accuracy when designing preventive vaccines. This study investigated the use of deep sequencing by the next-generation sequencing (NGS) Illumina MiSeq Platform to obtain complete genome sequence information from influenza virus isolates. Read More

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November 2013

Quality-score refinement of SSU rRNA gene pyrosequencing differs across gene region for environmental samples.

Microb Ecol 2012 Aug 5;64(2):499-508. Epub 2012 Apr 5.

Department of Microbiology, Center for Biofilm Engineering, Montana State University, 366 EPS Building, Bozeman, MT 59717, USA.

Due to potential sequencing errors in pyrosequencing data, species richness and diversity indices of microbial systems can be miscalculated. The "traditional" sequence refinement method is not sufficient to account for overestimations (e.g. Read More

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Position-dependent effects of locked nucleic acid (LNA) on DNA sequencing and PCR primers.

Nucleic Acids Res 2006 28;34(20):e142. Epub 2006 Oct 28.

Celadon Laboratories Inc., Technology Growth Center 6525 Belcrest Road, Suite 500, Hyattsville, MD 20782, USA.

Genomes are becoming heavily annotated with important features. Analysis of these features often employs oligonucleotides that hybridize at defined locations. When the defined location lies in a poor sequence context, traditional design strategies may fail. Read More

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December 2006
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