10,255 results match your criteria pyruvic acid

Evaluation of the Hypoglycemic Activity of by Targeting Protein Tyrosine Phosphatase 1B.

Front Pharmacol 2021 14;12:661803. Epub 2021 May 14.

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.

() Pers. is one of six wild edible mushrooms that are widely used by Asian and European countries for their nutritional value. The present study assessed the anti-diabetic potential of methanolic extract (100 mg/kg body weight) on streptozotocin (STZ)-induced diabetic mice. Read More

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Ethyl pyruvate ameliorates heat stroke-induced multiple organ dysfunction and inflammatory responses by induction of stress proteins and activation of autophagy in rats.

Int J Hyperthermia 2021 ;38(1):862-874

Department and Graduate Institute of Pharmacology, National Defense Medical Center, Taipei, Taiwan.

Heat stroke (HS) elicits the systemic inflammatory responses that result in multiple organ dysfunction (MOD). Heat shock response and autophagy are activated during heat stress for removal of damaged organelles and proteins, emerging as a major regulator of cellular homeostasis. Ethyl pyruvate (EP) is a derivative of pyruvic acid and possesses antioxidant and anti-inflammatory effects. Read More

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January 2021

Comprehensive Volatilome and Metabolome Signatures of Colorectal Cancer in Urine: A Systematic Review and Meta-Analysis.

Cancers (Basel) 2021 May 21;13(11). Epub 2021 May 21.

Oncology Department, Hospital Universitari Sant Joan de Reus, Institut d'Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili (URV), 43204 Reus, Spain.

To increase compliance with colorectal cancer screening programs and to reduce the recommended screening age, cheaper and easy non-invasiveness alternatives to the fecal immunochemical test should be provided. Following the PRISMA procedure of studies that evaluated the metabolome and volatilome signatures of colorectal cancer in human urine samples, an exhaustive search in PubMed, Web of Science, and Scopus found 28 studies that met the required criteria. There were no restrictions on the query for the type of study, leading to not only colorectal cancer samples versus control comparison but also polyps versus control and prospective studies of surgical effects, CRC staging and comparisons of CRC with other cancers. Read More

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Toxic responses of liver in Lateolabrax maculatus during hypoxia and re-oxygenation.

Aquat Toxicol 2021 Jul 21;236:105841. Epub 2021 Apr 21.

Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510220, PR China; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou, 510000, P.R. China. Electronic address:

Estuarine environmental have been reported to undergo significant fluctuations in oxygen concentrations with hypoxic conditions and subsequent re-oxygenation events being of significant concern for resident fish populations. In this study we assessed the toxicological effects of hypoxia and re-oxygenation on the liver of hypoxia-sensitive spotted sea bass (Lateolabrax maculatus) that were exposed to hypoxia (1.17 mg/L dissolved oxygen) for 12 h and then re-oxygenated for 12 h. Read More

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Temperature-responded biological fitness of carbendazim-resistance Fusarium graminearum mutants conferring the F167Y, E198K and E198L substitutions.

Plant Dis 2021 May 19. Epub 2021 May 19.

College of Plant Protection,Nanjing Agricultural University, Plant Pathology, No.1, Weigang, Nanjing, Jiangsu, China, 210095;

Understanding the effects of temperature on Fusarium graminearum infection can provide the theoretical guidance for chemical control of Fusarium head blight (FHB).Here, we evaluated the effects of various temperatures on biological fitness development of wild-type sensitive strain 2021 and carbendazim-resistance mutants conferring β2-tubulin substitutions F167Y, E198K and E198L. The results showed that mycelial growth and conidiation of four strains increased with the increase of the temperature between 10°C and 25°C. Conidia of F167Y displayed strong adaptability to low temperature. Read More

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Developmental toxicity of procymidone to larval zebrafish based on physiological and transcriptomic analysis.

Comp Biochem Physiol C Toxicol Pharmacol 2021 May 15;248:109081. Epub 2021 May 15.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address:

As a broad-spectrum with low toxicity, procymidone (PCM), is widely used in agriculture and frequently observed in aquatic system, which may cause some impacts on aquatic organisms. Here, to determine the developmental toxicity of PCM, embryonic and larval zebrafish were exposed to PCM at 0, 1, 10, 100 μg/L in dehydrogenated natural water containing 0.01% acetone for 7 days. Read More

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Huanglian Jiedu Decoction in the Treatment of the Traditional Chinese Medicine Syndrome "Shanghuo"-An Intervention Study.

Front Pharmacol 2021 30;12:616318. Epub 2021 Apr 30.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

"Shanghuo" ("excessive internal heat") is caused by exuberant endogenous fire, which does not have a comprehensive and systematic traditional Chinese medicine theory. In previous study, we had evaluated the therapeutic effect of Huanglian Jiedu Decoction (HLJDD) (granule) on patients with "Shanghuo", however, the specific mechanism was not clear, which need further exploration. To explain its intervention mechanism, we select 57 patients with oral diseases caused by "Shanghuo" and 20 health volunteers to divide into oral disease group, HLJDD intervention group and healthy control group. Read More

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Oleic acid in Angus and Hanwoo (Korean native cattle) fat reduced the fatty acid synthase activity in rat adipose tissues.

J Anim Sci Technol 2021 Mar 31;63(2):380-393. Epub 2021 Mar 31.

Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea.

This study aimed to determine the blood lipid profiles, fatty acid composition, and lipogenic enzyme activities in rat adipose tissues as affected by the Angus beef fat (ABF) and Hanwoo beef fat (HBF) containing high oleic acid (OA) content. We assigned 60 Sprague Dawley rats with a mean bodyweight of 249 ± 3.04 g to three groups (n = 20 each) to receive diets containing 7% coconut oil (CON), 7% ABF, or 7% HBF. Read More

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[Application of chronological lifespan in the construction of Escherichia coli cell factories].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1277-1286

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.

Microbial cell factories capable of producing valuable chemicals from renewable feedstocks provide a promising alternative towards sustainability. However, environmental stress remarkably affects the performance of microbial cell factories. By extending the chronological lifespan of microbial cells, the performance of microbial cell factories can be greatly improved. Read More

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Estimation of serum pyruvic acid levels in oral squamous cell carcinoma.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):585. Epub 2021 Jan 9.

Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.

Introduction: Cancer cells generally exhibit increased glycolysis for adenosine triphosphate generation (the Warburg effect) due to mitochondrial respiration injury and hypoxia, which are frequently associated with resistance to therapeutic agents. Pyruvic acid is produced as an end product of glycolysis along with lactic acid. At room temperature, lactic acid converts into pyruvic acid as it is chemically unstable. Read More

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January 2021

Total flavonoids of Ameliorated Bile Acid Metabolism Dysfunction in Diabetes Mellitus.

Evid Based Complement Alternat Med 2021 12;2021:6675567. Epub 2021 Apr 12.

Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China.

is one of the common traditional Chinese medicines used to treat diabetes. However, the underlying mechanism is not fully understood. Flavones are a class of active components that have been reported to exert various activities. Read More

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Extracts Stimulate IAA Biosynthesis in ZZ21 via the Indole-3-pyruvic Acid Pathway.

Microorganisms 2021 Apr 30;9(5). Epub 2021 Apr 30.

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.

Inter-organismal metabolites play important roles in regulating organism behavior and the communication between organisms. Nematodes, the most abundant animals on earth, are crucial participants in soil ecosystems through their interactions with microbes. For example, bacterial-feeding nematodes increase the activity of indole-3-acetic acid (IAA)-producing bacteria and the IAA content in soil. Read More

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D-Rhamnan and Pyruvate-Containing Teichuronic Acid from the Cell Wall of Rathayibacter sp. VKM Ac-2759.

Biochemistry (Mosc) 2021 Apr;86(4):506-516

All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.

Rathayibacter sp. VKM Ac-2759 (family Microbacteriaceae, class Actinobacteria) contains two glycopolymers in the cell wall. The main chain of rhamnan, glycopolymer 1, is built from the repeating tetrasaccharide units carrying terminal arabinofuranose residues at the non-reducing end, →3)-α-[α-D-Araf-(1→2)]-D-Rhap-(1→2)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→. Read More

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Untargeted lipidomics analysis of Mori Fructus polysaccharide on acute alcoholic liver injury in mice using ultra performance liquid chromatography-quadrupole-orbitrap-high resolution mass spectrometry.

Int Immunopharmacol 2021 May 1;97:107521. Epub 2021 May 1.

Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Engineering Laboratory for Quality Control and Evaluation Technology of Medicine, Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China. Electronic address:

Ethnopharmacological Relevance: Mori Fructus (MF) is a traditional Chinese medicine with long application history, which has protective effects on liver diseases. Mori Fructus polysaccharide (MFP) is one of the active ingredients of MF and possesses therapeutic effects against acute alcoholic liver injury. Recent researches have reported that the therapeutic effects of MFP might be related to the regulation of several lipid metabolic pathways. Read More

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A bacterial effector protein uncovers a plant metabolic pathway involved in tolerance to bacterial wilt disease.

Mol Plant 2021 May 1. Epub 2021 May 1.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences; Chinese Academy of Sciences, Shanghai 201602, China. Electronic address:

Bacterial wilt caused by the soil-borne plant pathogen Ralstonia solanacearum is a devastating disease worldwide. Upon plant colonization, R. solanacearum replicates massively, causing plant wilting and death; collapsed infected tissues then serve as a source of inoculum. Read More

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Physicochemical Properties and Effects of Fruit Pulps from the Amazon Biome on Physiological Parameters in Rats.

Nutrients 2021 Apr 28;13(5). Epub 2021 Apr 28.

Department of Engineering and Food Technology, São Paulo State University, R. Cristóvão Colombo, 2265-Jardim Nazareth, 15054-000 São José do Rio Preto, Brazil.

This study aimed to analyze the physicochemical characteristics and the effects of Amazonian pulp fruits consumption, such as araçá-boi (), abiu grande (), araticum (), biri-biri ( L.), and yellow mangosteen (), on hematologic, metabolic, renal, and hepatic function parameters in Wistar rats ( = 10 rats/group). The pulp of abiu had the highest levels of soluble solids, sugars, and pH. Read More

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Untargeted Metabolomics Insights into Newborns with Congenital Zika Infection.

Pathogens 2021 Apr 13;10(4). Epub 2021 Apr 13.

Departamento de Química Analítica, Instituto de Química, Universidade Federal da Bahia, Rua Barao de Jeremoabo, 147, Salvador, BA 40170-115, Brazil.

Zika virus (ZIKV), an emerging virus belonging to the Flaviviridae family, causes severe neurological clinical complications and has been associated with Guillain-Barré syndrome, fetal abnormalities known collectively as congenital Zika syndrome, and microcephaly. Studies have shown that ZIKV infection can alter cellular metabolism, directly affecting neural development. Brain growth requires controlled cellular metabolism, which is essential for cell proliferation and maturation. Read More

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Participating mechanism of a major contributing gene ysnE for auxin biosynthesis in Bacillus amyloliquefaciens SQR9.

J Basic Microbiol 2021 Jun 29;61(6):569-575. Epub 2021 Apr 29.

Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, China.

The phytohormone indole-3-acetic acid (IAA) has been demonstrated to contribute to the plant growth-promoting effect of rhizobacteria, but the IAA biosynthesis pathway in rhizobacteria remains unclear. The ysnE gene, encoding a putative tryptophan acetyltransferase, has been demonstrated to be involved in and strongly contribute to IAA production in Bacillus, but the mechanism is unknown. In this study, to investigate how ysnE participates in IAA biosynthesis in the plant growth-promoting rhizobacterium Bacillus amyloliquefaciens SQR9, differences in the produced IAA biosynthesis intermediates between wild-type SQR9 and ΔysnE were analyzed and compared, and the effects of different intermediate compounds on the production of IAA and the accumulation of other intermediates were also investigated. Read More

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An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats.

AMB Express 2021 Apr 21;11(1):58. Epub 2021 Apr 21.

Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.

The present study aimed at isolation of endophytic basidiomycetous fungi and evaluation of their in-vitro and in-vivo antidiabetic potential. Preliminary screening for in-vitro activity was carried out using α-glucosidase inhibition assay. An endophytic isolate Sch1 (isolated from Aloe vera), identified to be Schizophyllum commune Fr. Read More

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Expression of pyruvate carboxylase in cultured human astrocytoma, glioblastoma and neuroblastoma cells.

Gen Physiol Biophys 2021 Mar;40(2):127-135

Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.

Pyruvate carboxylase (PC) is an enzyme catalyzing the conversion of pyruvate to oxaloacetate, which possesses anaplerotic role in cellular metabolism. The expression of PC was confirmed in cells of several cancer types, in which it ensures several cellular functions, such as growth and division. To investigate the expression of PC in human astrocytoma, glioblastoma and neuroblastoma cells we applied the immunodetection methods. Read More

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Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants.

Appl Environ Microbiol 2021 Jun 11;87(13):e0048721. Epub 2021 Jun 11.

Department of Microbiology, University of Georgia, Athens, Georgia, USA.

Altering metabolic flux at a key branch point in metabolism has commonly been accomplished through gene knockouts or by modulating gene expression. An alternative approach to direct metabolic flux preferentially toward a product is decreasing the activity of a key enzyme through protein engineering. In Escherichia coli, pyruvate can accumulate from glucose when carbon flux through the pyruvate dehydrogenase complex is suppressed. Read More

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Antidiabetic effect of seeds extract in streptozotocin-induced diabetic mice: a statistical evaluation.

J Investig Med 2021 Apr 12. Epub 2021 Apr 12.

Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran.

Undoubtedly, identification of the chemical composition of organic extracts or secondary metabolites of plant materials and evaluation of their potential bioactivity are among the main objectives of natural products-based investigations. In the present study, we report the chemical composition and antidiabetic activity of (Family Fabaceae) seeds extract (SPE) for the first time. First, the plant seeds were macerated in ethanol. Read More

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Effects of polyethylene microplastics on the microbiome and metabolism in larval zebrafish.

Environ Pollut 2021 Aug 31;282:117039. Epub 2021 Mar 31.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China. Electronic address:

Various microplastics (MPs) are found in the environment and organisms. MP residues in organisms can affect health; however, their impacts on metabolism in aquatic organisms remain unclear. In this study, zebrafish embryos were exposed to polyethylene MPs with sizes ranging from 1 to 4 μm at concentrations of 0, 10, 100, and 1000 μg/L for 7 days. Read More

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Association of structural variation with cardiometabolic traits in Finns.

Am J Hum Genet 2021 04;108(4):583-596

McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO 63108, USA; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA. Electronic address:

The contribution of genome structural variation (SV) to quantitative traits associated with cardiometabolic diseases remains largely unknown. Here, we present the results of a study examining genetic association between SVs and cardiometabolic traits in the Finnish population. We used sensitive methods to identify and genotype 129,166 high-confidence SVs from deep whole-genome sequencing (WGS) data of 4,848 individuals. Read More

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Single-Cell Oxidative Stress Events Revealed by a Renewable SERS Nanotip.

ACS Sens 2021 04 30;6(4):1663-1670. Epub 2021 Mar 30.

State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.

A nanotip sensitive to reactive oxygen species (ROS) and NAD/NADH (oxidized/reduced forms of nicotinamide adenine dinucleotide) was designed and prepared to identify the redox events in a single living cell by surface-enhanced Raman scattering (SERS) spectroscopy. The nanotips were prepared by the one-step laser-induced Ag growth and deposition. A redox-reversible Raman reporter, 4-mercaptophenol (4-MP), was employed for the nanotip decoration along with the Ag deposition. Read More

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Effects of dexmedetomidine on the function of distal organs and oxidative stress after lower limb ischaemia-reperfusion in elderly patients undergoing unilateral knee arthroplasty.

Br J Clin Pharmacol 2021 Mar 23. Epub 2021 Mar 23.

Department of Anaesthesiology, Southwest Hospital, Third Military Medical University (Army Medical University), 30 Gaotanyan Road, Chongqing, China.

Aims: This study aims to evaluate the effects of dexmedetomidine on organ function, inflammation response, and oxidative stress in elderly patients following iatrogenic lower limb ischaemia-reperfusion (IR) during unilateral total knee arthroplasty.

Methods: Following unilateral total knee arthroplasty, 54 elderly patients were randomized to receive either intraoperative intravenous injection of dexmedetomidine (n = 27) or equivalent volume of 0.9% saline (n = 27). Read More

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Risk of hepatitis-E virus infections among blood donors in a regional blood transfusion centre in western India.

Transfus Med 2021 Jun 18;31(3):193-199. Epub 2021 Mar 18.

Department of Transfusion Medicine, Surat Raktadan Kendra and Research Centre, Surat, India.

Background: Hepatitis-E virus (HEV) is an emerging infectious threat to blood safety. The enormity of the transmission of HEV and its clinical consequence are issues currently under debate. This study aimed to evaluate the prevalence of HEV-RNA in blood donors in western India. Read More

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Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides).

Sci Rep 2021 Mar 18;11(1):6230. Epub 2021 Mar 18.

Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.

The purpose of subject was to explore the optimum protein requirement of juvenile grouper (Epinephelus coioides). In the test, 450 juveniles with an average weight (10.02 ± 0. Read More

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Metabonomics of white adipose tissue and brown adipose tissue in Tupaia belangeri during cold acclimation.

Comp Biochem Physiol Part D Genomics Proteomics 2021 Jun 3;38:100823. Epub 2021 Mar 3.

Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Science, Yunnan Normal University, Kunming 650500, Yunnan, China. Electronic address:

In the present study, liquid chromatography-mass spectrometer (LC-MS) was used to perform untargeted metabolomics analysis of white adipose tissue (WAT) and brown adipose tissue (BAT) in Tupaia belangeri during cold acclimation. Differences in biochemical composition between WAT and BAT were compared. Clarifying how the two adipose tissues respond to the lower temperature in terms of metabolomics, which elucidate the metabolic process and energy homeostasis regulation mechanism in T. Read More

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Aberrant lactate dehydrogenase A signaling contributes metabolic signatures in pancreatic cancer.

Ann Transl Med 2021 Feb;9(4):358

Department of Clinical Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Background: Pancreatic cancer (PC) has the lowest 5-year survival rate; therefore, new early screening methods and therapeutic targets are still urgently required. Emerging technologies such as metabolomic-based liquid biopsy may contribute to the field. We found aberrant lactate dehydrogenase A (LDHA) signaling to be an unfavorable biomarker for PC. Read More

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February 2021