13,579 results match your criteria pyruvate kinase


Iminostilbene, a novel small-molecule modulator of PKM2, suppresses macrophage inflammation in myocardial ischemia-reperfusion injury.

J Adv Res 2021 Mar 9;29:83-94. Epub 2020 Sep 9.

Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China.

Introduction: Inflammation is a key factor in myocardial ischemia/reperfusion (MI/R) injury. Targeting leucocyte-mediated inflammation is an important strategy for MI/R therapy. Iminostilbene (ISB), a simple dibenzoazepine small molecule compound, has a strong anti-neurodegenerative effect. Read More

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Hypoglycemic effect of root extract and its synergism with extract.

Food Sci Nutr 2021 Apr 26;9(4):2075-2085. Epub 2021 Feb 26.

School of Forestry Northeast Forestry University Harbin China.

(dandelion) and are traditional medicinal and edible plants with high nutritional value. In this study, the synergistic hypoglycemic effect of DRE and extract (RAE) was evaluated. Our results showed that water extract of dandelion (DRE-w), mainly containing polysaccharides (63. Read More

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circCCDC66 promotes thyroid cancer cell proliferation, migratory and invasive abilities and glycolysis through the miR-211-5p/PDK4 axis.

Oncol Lett 2021 May 26;21(5):416. Epub 2021 Mar 26.

Department of General Surgery, Qingdao Municipal Hospital, Qingdao University School of Medicine, Shandong, Qingdao 266000, P.R. China.

Cancer metastasis is the main cause of death in cancer patients, including patients with thyroid cancer (TC). TC is the most common malignant endocrine tumour. In the recent years, increasing evidence has demonstrated that circular RNAs (circRNAs) serve a significant role in the development of many types of human cancer. Read More

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Posttranslational modification of pyruvate kinase type M2 (PKM2): novel regulation of its biological roles to be further discovered.

J Physiol Biochem 2021 Apr 9. Epub 2021 Apr 9.

Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.

PKM2, pyruvate kinase type M2, has been shown to play a key role in aerobic glycolysis and to regulate the malignant behaviors of cancer cells. Recently, PKM2 has been revealed to hold dual metabolic and nonmetabolic roles. Working as both a pyruvate kinase with catalytic activity and a protein kinase that phosphorylates its substrates, PKM2 stands at the crossroads of glycolysis and tumor growth. Read More

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Hepatoprotective Effects of Polydatin-Loaded Chitosan Nanoparticles in Diabetic Rats: Modulation of Glucose Metabolism, Oxidative Stress, and Inflammation Biomarkers.

Biochemistry (Mosc) 2021 Feb;86(2):179-189

Molecular Physiology Division, Faculty of Science, Beni-Suef University, Beni-Suef, 62511, Egypt.

Polydatin (PD) has a broad range of pharmacological activities; however, its effects on diabetic liver damage are poorly studies. This work is aimed to explore possible protective effects of polydatin-loaded chitosan nanoparticles (PD-CSNPs) or PD against liver damage associated with diabetes. Diabetes was induced in rats using nicotinamide/streptozotocin treatment. Read More

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February 2021

Trehalose alleviates high-temperature stress in Pleurotus ostreatus by affecting central carbon metabolism.

Microb Cell Fact 2021 Apr 7;20(1):82. Epub 2021 Apr 7.

Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Background: Trehalose, an intracellular protective agent reported to mediate defense against many stresses, can alleviate high-temperature-induced damage in Pleurotus ostreatus. In this study, the mechanism by which trehalose relieves heat stress was explored by the addition of exogenous trehalose and the use of trehalose-6-phosphate synthase 1 (tps1) overexpression transformants.

Results: The results suggested that treatment with exogenous trehalose or overexpression of tps1 alleviated the accumulation of lactic acid under heat stress and downregulated the expression of the phosphofructokinase (pfk) and pyruvate kinase (pk) genes, suggesting an ameliorative effect of trehalose on the enhanced glycolysis in P. Read More

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Profiling the interaction of a novel toxic pyruvate dehydrogenase kinase inhibitor with human serum albumin.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 23;256:119733. Epub 2021 Mar 23.

School of Food & Biological Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address:

To discover novel pyruvate dehydrogenase kinase (PDK) inhibitors, a new compound 2,2-dichloro-1-(4-((4-isopropylphenyl)amino)-3-nitrophenyl)ethan-1-one, namely XB-1 was identified, which inhibited PDK activity with a half maximal inhibitory concentration (IC) value of 337.0 nM, and reduced A549 cell proliferation with a half maximal effective concentration (EC) value of 330.0 nM. Read More

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FoxO1 inhibition alleviates type 2 diabetes-related diastolic dysfunction by increasing myocardial pyruvate dehydrogenase activity.

Cell Rep 2021 Apr;35(1):108935

Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada; Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada; Cardiovascular Research Centre, University of Alberta, Edmonton, AB, Canada. Electronic address:

Type 2 diabetes (T2D) increases the risk for diabetic cardiomyopathy and is characterized by diastolic dysfunction. Myocardial forkhead box O1 (FoxO1) activity is enhanced in T2D and upregulates pyruvate dehydrogenase (PDH) kinase 4 expression, which inhibits PDH activity, the rate-limiting enzyme of glucose oxidation. Because low glucose oxidation promotes cardiac inefficiency, we hypothesize that FoxO1 inhibition mitigates diabetic cardiomyopathy by stimulating PDH activity. Read More

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Passiflora edulis (var. Flavicarpa) Juice Supplementation Mitigates Isoproterenol-induced Myocardial Infarction in Rats.

Plant Foods Hum Nutr 2021 Apr 6. Epub 2021 Apr 6.

Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, 695581, India.

The common reason for mortality globally is myocardial infarction. The study aimed to evaluate Passiflora edulis (PE) fruit juice potential in the experimental isoproterenol (ISO) treated rat model to manage myocardial injury. ISO (20 mg/100 g body weight) treated rats showed a significant increment in serum marker enzymes lactate dehydrogenase (LDH) and creatinine kinase (CK), serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), serum alkaline phosphatase (ALP) and serum acid phosphatase (ACP) activity. Read More

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The pyruvate kinase activator mitapivat reduces hemolysis and improves anemia in a β-thalassemia mouse model.

J Clin Invest 2021 Apr 6. Epub 2021 Apr 6.

Department of Medicine, University of Verona, Verona, Italy.

Anemia in β-thalassemia is related to ineffective erythropoiesis and reduced red cell survival. Excess free heme and accumulation of unpaired α-globin chains impose substantial oxidative stress on β-thalassemic erythroblasts and erythrocytes, impacting cell metabolism. We hypothesized that increased pyruvate kinase activity induced by mitapivat (AG-348) in the Hbbth3/+ mouse model for β-thalassemia would reduce chronic hemolysis and ineffective erythropoiesis through stimulation of red cell glycolytic metabolism. Read More

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Detection of Escherichia coli using luminometer with pyruvate kinase.

J Mol Recognit 2021 Apr 6:e2896. Epub 2021 Apr 6.

National Ocean Technology Center, Tianjin, China.

Portable and quantitative detection of Escherichia coli (E. coli) has the potential to reform clinical diagnostics, food safety, and environmental monitoring. At present, most commercial devices used for pathogen detection have disadvantages such as expensive, highly complex operations, or limited detection specificity. Read More

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LINC00662 modulates cervical cancer cell proliferation, invasion, and apoptosis via sponging miR-103a-3p and upregulating PDK4.

Mol Carcinog 2021 Apr 5. Epub 2021 Apr 5.

Gynecology Department, Xuzhou First People's Hospital, Xuzhou, Jiangsu, China.

Cervical cancer (CC) is one of the most common cancers among women with high recurrence rates all over the world. Recently, the molecular mechanism of CC has been gradually uncovered in accumulating reports. This study aimed to investigate the function and upstream regulation mechanism of pyruvate dehydrogenase kinase 4 (PDK4) in CC cells, which was verified as an oncogene in several cancers. Read More

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Circ_0091579 enhances the malignancy of hepatocellular carcinoma via miR-1287/PDK2 axis.

Open Life Sci 2021 25;16(1):69-83. Epub 2021 Jan 25.

Department of General Surgery, Ankang People's Hospital of Shanxi Province, Ankang, 6-1-3302, Shifu Courtyard, High-Tech Zone, Ankang 725000, Shanxi Province, China.

Several articles have indicated that circular RNAs are involved in pathogenesis of human cancers. Nevertheless, the role of circ_0091579 in hepatocellular carcinoma (HCC) progression remains to be revealed. Quantitative reverse transcriptase polymerase chain reaction was carried out to examine the expression of circ_0091579 and miR-1287. Read More

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January 2021

Anaerobic fermentation metabolism of Moorella thermoacetica inhibited by copper nanoparticles: Comprehensive analyses of transcriptional response and enzyme activity.

Water Res 2021 Mar 22;197:117081. Epub 2021 Mar 22.

State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.

Engineered nanoparticles are observed to be released into the environment and ended up in wastewater treatment plants. It has been reported that these nanoparticles in sewage might have a toxic effect on microorganisms, and thus affect anaerobic microbial fermentation. However, the mechanisms involved in nanoparticles-induced effects on the anaerobic acidification process and its related bacterial metabolism are still unclear. Read More

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A Novel Therapeutic Target, BACH1, Regulates Cancer Metabolism.

Cells 2021 Mar 12;10(3). Epub 2021 Mar 12.

Department of Biochemistry and Molecular Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.

BTB domain and CNC homology 1 (BACH1) is a transcription factor that is highly expressed in tumors including breast and lung, relative to their non-tumor tissues. BACH1 is known to regulate multiple physiological processes including heme homeostasis, oxidative stress response, senescence, cell cycle, and mitosis. In a tumor, BACH1 promotes invasion and metastasis of cancer cells, and the expression of BACH1 presents a poor outcome for cancer patients including breast and lung cancer patients. Read More

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Effect of a Shock Micro-Cycle on Biochemical Markers in University Soccer Players.

Int J Environ Res Public Health 2021 Mar 30;18(7). Epub 2021 Mar 30.

Sánchez-Muñoz OE, Physical Education and Sports Program, Research Group on Physical Activity and Health Physiology (GIFAS), University of Quindío, Armenia 63000, Colombia.

This study aimed to examine various biochemical biomarkers changes during a shock micro-cycle in soccer players from a university team. The study had 22 players (age: 22 ± 3 years; body mass: 68.6 ± 7. Read More

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Non-Metabolic Functions of PKM2 Contribute to Cervical Cancer Cell Proliferation Induced by the HPV16 E7 Oncoprotein.

Viruses 2021 Mar 8;13(3). Epub 2021 Mar 8.

Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.

Pyruvate kinase M2 (PKM2) mainly catalyzes glycolysis, but it also exerts non-glycolytic functions in several cancers. While it has been shown to interact with the human papillomavirus 16 (HPV16) E7 oncoprotein, the functional significance of PKM2 in HPV-associated cervical cancer has been elusive. Here, we show that HPV16 E7 increased the expression of PKM2 in cervical cancer cells. Read More

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Two mitochondrial phosphatases, PP2c63 and Sal2, are required for posttranslational regulation of the TCA cycle in Arabidopsis.

Mol Plant 2021 Mar 30. Epub 2021 Mar 30.

Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany; Center of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria. Electronic address:

Protein phosphorylation is a well-established post-translational mechanism that regulates protein functions and metabolic pathways. It is known that several plant mitochondrial proteins are phosphorylated in a reversible manner. However, the identity of the protein kinases/phosphatases involved in this mechanism and their role in the regulation of the TCA cycle remain unclear. Read More

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Metabolic Reprogramming and Inflammatory Response Induced by D-Lactate in Bovine Fibroblast-Like Synoviocytes Depends on HIF-1 Activity.

Front Vet Sci 2021 16;8:625347. Epub 2021 Mar 16.

Laboratory of Inflammation Pharmacology, Faculty of Veterinary Sciences, Institute of Pharmacology and Morphophysiology, Universidad Austral de Chile, Valdivia, Chile.

Acute ruminal acidosis (ARA) occurs after an excessive intake of rapidly fermentable carbohydrates and is characterized by the overproduction of D-lactate in the rumen that reaches the bloodstream. Lameness presentation, one of the primary consequences of ARA in cattle, is associated with the occurrence of laminitis and aseptic polysynovitis. Fibroblast-like synoviocytes (FLS) are predominant cells of synovia and play a key role in the pathophysiology of joint diseases, thus increasing the chances of the release of pro-inflammatory cytokines. Read More

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Impact of the Whole Genome Duplication Event on PYK Activity and Effects of a PYK1 Mutation on Metabolism in .

Front Mol Biosci 2021 16;8:656461. Epub 2021 Mar 16.

Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States.

Evolution of aerobic fermentation (crabtree effect) in yeast is associated with the whole genome duplication (WGD) event, suggesting that duplication of certain genes may have altered yeast metabolism. The pyruvate kinase (PYK) gene is associated with alterations in cell metabolism, and duplicated during the WGD, generating PYK1 and PYK2. Thus, the impact of WGD on PYK activity and role of PYK in yeast metabolism were explored. Read More

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Chlorogenic acid promotes osteogenic differentiation of human dental pulp stem cells via Wnt signaling.

Stem Cells Dev 2021 Apr 1. Epub 2021 Apr 1.

Nanchang University, 47861, Affiliated Stomatological Hospital of Nanchang University; The Key Laboratory of Oral Biomedicine, Nanchang, jiangxi, China, 330006;

Periodontal disease (PD) is one of the main causes of periodontal bone resorption and tooth loss in adults. How to repair the alveolar bone effectively has always been a challenge. This study was designed to clarify the effects and the underlying molecular mechanisms of chlorogenic acid (CGA) on osteogenic differentiation of human dental pulp stem cells (hDPSCs). Read More

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Axial functionalisation of photoactive diazido platinum(iv) anticancer complexes.

Inorg Chem Front 2020 Oct 26;7(19):3533-3540. Epub 2020 Aug 26.

Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.

Mono-axial functionalised octahedral diazido Pt(iv) complexes trans, trans, trans-[Pt(py)(N)(OR)(OR)] (OR = OH and OR = anticancer agent coumarin-3 carboxylate (cou, ), pyruvate dehydrogenase kinase (PDK) inhibitors 4-phenylbutyrate (PhB, ) or dichloroacetate (DCA, )), and their di-axial functionalised analogues with OR = DCA and OR = cou (), PhB (), or DCA () have been synthesised and characterised, including the X-ray crystal structures of complexes and . These complexes exhibit dark stability and have the potential to generate cytotoxic Pt(ii) species and free radicals selectively in cancer cells when irradiated. Mono-functionalised complexes showed higher aqueous solubility and more negative reduction potentials. Read More

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October 2020

Circular RNA hsa_circ_0005397 promotes hepatocellular carcinoma progression by regulating miR-326/PDK2 axis.

J Gene Med 2021 Mar 30:e3332. Epub 2021 Mar 30.

Department of Digestive Medicine, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China.

Introduction: Circular RNAs (circRNAs) have demonstrated to be associated with initiation and progression of cancer. However, the biological functions and underlying mechanism of hsa_circ_0005397 in hepatocellular carcinoma (HCC) have been fully elucidated.

Methods: HE staining was used to assess histological changes. Read More

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BAY 60-6583 Enhances the Antitumor Function of Chimeric Antigen Receptor-Modified T Cells Independent of the Adenosine A2b Receptor.

Front Pharmacol 2021 12;12:619800. Epub 2021 Mar 12.

Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.

Chimeric antigen receptor (CAR) T cells are powerful in eradicating hematological malignancies, but their efficacy is limited in treating solid tumors. One of the barriers is the immunosuppressive response induced by immunomodulatory signaling pathways. Pharmacological targeting of these immunosuppressive pathways may be a simple way to improve the efficacy of CAR T cells. Read More

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Antioxidant activities of peptides derived from mutton ham, Xuanwei ham and Jinhua ham.

Food Res Int 2021 Apr 1;142:110195. Epub 2021 Feb 1.

School of Food Science and Technology, Shihezi University, Xinjiang Autonomus Region, Shihezi, China.

The aim of the study was to evaluate antioxidant activity of crude peptides with molecular weight less than 3 KDa extracted from Xuanwei ham, Jinhua ham and mutton ham. UPLC-Q-TOF-MS/MS was used for composition analysis of peptides and homologous protein matching. Further, crude peptide (<3 KDa) was purified using G-15 gel filtration chromatography, and the main antioxidant peptide identified. Read More

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BCKDK regulates the TCA cycle through PDC in the absence of PDK family during embryonic development.

Dev Cell 2021 Mar 23. Epub 2021 Mar 23.

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address:

Pyruvate dehydrogenase kinases (PDK1-4) inhibit the TCA cycle by phosphorylating pyruvate dehydrogenase complex (PDC). Here, we show that PDK family is dispensable for murine embryonic development and that BCKDK serves as a compensatory mechanism by inactivating PDC. First, we knocked out all four Pdk genes one by one. Read More

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SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.

Nat Commun 2021 03 25;12(1):1876. Epub 2021 Mar 25.

Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.

Viruses hijack host cell metabolism to acquire the building blocks required for replication. Understanding how SARS-CoV-2 alters host cell metabolism may lead to potential treatments for COVID-19. Here we profile metabolic changes conferred by SARS-CoV-2 infection in kidney epithelial cells and lung air-liquid interface (ALI) cultures, and show that SARS-CoV-2 infection increases glucose carbon entry into the TCA cycle via increased pyruvate carboxylase expression. Read More

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Role of cardiac hypoxia in the pathogenesis of sudden death syndrome in broiler chickens - A metabolic and molecular study.

Acta Vet Hung 2021 Mar 19. Epub 2021 Mar 19.

3Poultry Diseases Research Center, School of Veterinary Medicine, Shiraz University, Shiraz, Islamic Republic of Iran.

Sudden death syndrome (SDS) is an economically important disorder in broiler chickens with unknown aetiology. The aim of the present study was to evaluate the metabolic and molecular alterations related to hypoxia in the myocardium of broiler chickens with SDS. Samples from the cardiac muscle of internal control broiler chickens (ICs) (n = 36) and chickens having died of SDS (n = 36) were obtained during the rearing period. Read More

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