163 results match your criteria pvy strains

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains.

Plants (Basel) 2021 Apr 12;10(4). Epub 2021 Apr 12.

INRAE, University of Bordeaux, UMR BFP, 33140 Villenave d'Ornon, France.

In recent years, high throughput sequencing (HTS) has brought new possibilities to the study of the diversity and complexity of plant viromes. Mixed infection of a single plant with several viruses is frequently observed in such studies. We analyzed the virome of 10 tomato and sweet pepper samples from Slovakia, all showing the presence of potato virus Y (PVY) infection. Read More

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Extreme Resistance to Viruses in Potato and Soybean.

Front Plant Sci 2021 6;12:658981. Epub 2021 Apr 6.

Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, United States.

Plant pathogens, including viruses, negatively impact global crop production. Plants have evolved complex immune responses to pathogens. These responses are often controlled by nucleotide-binding leucine-rich repeat proteins (NLRs), which recognize intracellular, pathogen-derived proteins. Read More

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Occurrence and distribution of viruses infecting potato in Russia.

Lett Appl Microbiol 2021 Jul 4;73(1):64-72. Epub 2021 May 4.

Federal State Budget Scientific Institution, All-Russian Institute of Plant Protection (FSBSI VIZR), Saint Petersburg, Russia.

Potato viral disease has been a major problem in potato production worldwide including Russia. Here, we detected Potato Virus M (PVM), P (PVP), S (PVS), Y (PVY), and X (PVX) and Potato Leaf Roll Virus (PLRV) by RT-PCR on potato leaves and tubers from the Northwestern (NW), Volga (VF), and Far Eastern (FE) federal districts of Russia. Each sample was co-infected with up to five viruses. Read More

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Different potato virus Y strains frequently co-localize in single epidermal leaf cells and in the aphid stylet.

J Gen Virol 2021 Mar 12;102(3). Epub 2021 Mar 12.

USDA-ARS, Emerging Pests and Pathogen Research Unit, Ithaca, NY 14853-5904, USA.

Single aphids can simultaneously or sequentially acquire and transmit multiple potato virus Y (PVY) strains. Multiple PVY strains are often found in the same field and occasionally within the same plant, but little is known about how PVY strains interact in plants or in aphid stylets. Immuno-staining and confocal microscopy were used to examine the spatial and temporal dynamics of PVY strain mixtures (PVY and PVY or PVY and PVY) in epidermal leaf cells of 'Samsun NN' tobacco and 'Goldrush' potato. Read More

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Potato Virus Y Emergence and Evolution from the Andes of South America to Become a Major Destructive Pathogen of Potato and Other Solanaceous Crops Worldwide.

Viruses 2020 12 12;12(12). Epub 2020 Dec 12.

The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.

The potato was introduced to Europe from the Andes of South America in the 16th century, and today it is grown worldwide; it is a nutritious staple food eaten by millions and underpins food security in many countries. Unknowingly, potato virus Y (PVY) was also introduced through trade in infected potato tubers, and it has become the most important viral pathogen of potato. Phylogenetic analysis has revealed the spread and emergence of strains of PVY, including strains causing economically important diseases in tobacco, tomato and pepper, and that the virus continues to evolve with the relatively recent emergence of new damaging recombinant strains. Read More

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December 2020

Molecular and biological characterization of recombinant isolates of circulating in potato fields in Mexico.

Plant Dis 2020 Dec 2. Epub 2020 Dec 2.

University of Idaho, EPPN, 875 Perimeter Dr., MS 2329, Moscow, Idaho, United States, 83844-2329;

(PVY) is a significant threat to potato production in Mexico. The presence of recombinant strains of PVY circulating in potato has been reported in the country, but no systematic study on the genetic diversity of PVY in potato and prevalence of PVY strains has been conducted yet. Here, we report on a series of surveys in seed potato production areas in two states in Mexico, Chihuahua and Jalisco, between 2011 and 2019. Read More

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December 2020

Current-Season Infection With Ordinary and Recombinant Strains of Has Detrimental Effects on Chipping Potato Yield and Tuber Quality.

Plant Dis 2021 Feb 18;105(2):450-455. Epub 2020 Nov 18.

Department of Horticulture, University of Wisconsin, Madison, WI.

(PVY) is the most economically important virus infecting potatoes worldwide. Current-season spread of PVY occurs when aphids transmit the virus from infected to noninfected plants during the growing season. The impact of current-season PVY infection on yield and quality of chip processing potatoes is not well documented. Read More

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February 2021

The Recombinant Potato virus Y (PVY) Strain, PVY, Identified in Potato Fields in Victoria, Southeastern Australia.

Plant Dis 2020 Dec 15;104(12):3110-3114. Epub 2020 Oct 15.

University of Idaho, Department of EPPN, Moscow, ID 83844-2329, U.S.A.

Potato virus Y (PVY) is one of the main viruses affecting potato in Australia. However, molecular characterization of PVY isolates circulating in potato in different states of Australia has not yet been thoroughly conducted. Only nonrecombinant isolates of three biological PVY strains collected from potato were reported previously from Western Australia and one from Queensland. Read More

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December 2020

Small RNA profiling analysis of two recombinant strains of potato virus Y in infected tobacco plants.

Virus Res 2020 10 22;288:198125. Epub 2020 Aug 22.

Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, New Haven, CT 06511, United States. Electronic address:

Plant viral infections lead to accumulation of virus-derived small interfering RNAs (vsiRNAs) as a result of host defense mechanisms. High-throughput sequencing technology enables vsiRNA profiling analyses from virus infected plants, which provide important insights into virus-host interactions. Potato virus Y (PVY) is a detrimental plant pathogen that can infect a variety of solanaceous crops, e. Read More

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October 2020

Pest categorisation of potato virus Y (non-EU isolates).

EFSA J 2020 Jan 9;18(1):e05938. Epub 2020 Jan 9.

Following a request from the EU Commission, the Panel on Plant Health has addressed the pest categorisation of non-EU isolates of potato virus Y (PVY). The information currently available on geographical distribution, biology, epidemiology, potential entry pathways and potential additional impact of non-EU isolates of PVY, has been evaluated with regard to the criteria to qualify as a potential Union quarantine pest. Because non-EU isolates of PVY are absent from the EU, they do not meet one of the requirements to be regulated as a regulated non-quarantine pest (RNQP) (presence in the EU); as a consequence, the Panel decided not to evaluate the other RNQP criteria for these isolates. Read More

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January 2020

Analysis of codon usage bias in potato virus Y non-recombinant strains.

Virus Res 2020 09 30;286:198077. Epub 2020 Jun 30.

Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Igua 4225, 11400, Montevideo, Uruguay.

Potato virus Y (PVY) is a member of the genus Potyvirus, family Potyviridae, is considered one of the most devastating pest affecting economically important crops, such as potato, tobacco, tomato and pepper, representing a serious threat due to high incidence and worldwide distribution. Its economic significance as well as it biological and molecular complexities have aroused great attention, thus several studies have explore it genetic characteristics. However, little is known about PVY codon usage. Read More

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September 2020

Transmission modes affect the population structure of potato virus Y in potato.

PLoS Pathog 2020 06 23;16(6):e1008608. Epub 2020 Jun 23.

School of Integrative Plant Science, Plant Pathology and Plant-Microbe Biology Section, Cornell University, Ithaca, New York, United States of America.

Transmission is a crucial part of a viral life cycle and transmission mode can have an important impact on virus biology. It was demonstrated that transmission mode can influence the virulence and evolution of a virus; however, few empirical data are available to describe the direct underlying changes in virus population structure dynamics within the host. Potato virus Y (PVY) is an RNA virus and one of the most damaging pathogens of potato. Read More

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Potato Virus Y (PVY) Isolates from Represent Three Novel Recombinants Within the PVY Strain Group and Are Unable to Systemically Spread in Potato.

Phytopathology 2020 Sep 29;110(9):1588-1596. Epub 2020 Jul 29.

Department of EPPN, University of Idaho, Moscow, ID.

Tamarillo, or tree tomato (), is a perennial small tree or shrub species cultivated in subtropical areas for fresh fruit and juice production. In Ecuador, tamarillo orchards are affected by several viruses, with one previously identified as potato virus Y (PVY); however, the specific strain composition of PVY in tamarillo was not determined. In 2015 and 2016, eight tamarillo plants exhibiting symptoms of leaf drop, mosaic, and mottled fruit were sampled near Tumbaco and Quito, Ecuador. Read More

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September 2020

Screening for Resistance to PVY in Australian Potato Germplasm.

Genes (Basel) 2020 04 16;11(4). Epub 2020 Apr 16.

Agriculture Victoria, AgriBio, 5 Ring Road, La Trobe University, Bundoora, VIC 3083, Australia.

Potatoes are an important human food crop, but have a number of yield limiting factors, including disease susceptibility. (PVY) is found worldwide, and is one of the main virus problems for potato growers. PVY is transmitted by aphids and mechanically by machinery, tools and people, and symptoms are variable across cultivars and strains, including being symptomless in some cultivars. Read More

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Grafting alters tomato transcriptome and enhances tolerance to an airborne virus infection.

Sci Rep 2020 02 13;10(1):2538. Epub 2020 Feb 13.

Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari "Aldo Moro", Via Amendola 165/A, 70126, Bari, Italy.

Grafting of commercial tomato varieties and hybrids on the tomato ecotype Manduria resulted in high levels of tolerance to the infection of Sw5 resistance-breaking strains of tomato spotted wilt virus and of severe cucumber mosaic virus strains supporting hypervirulent satellite RNAs that co-determine stunting and necrotic phenotypes in tomato. To decipher the basis of such tolerance, here we used a RNAseq analysis to study the transcriptome profiles of the Manduria ecotype and of the susceptible variety UC82, and of their graft combinations, exposed or not to infection of the potato virus Y recombinant strain PVY-to. The analysis identified graft- and virus-responsive mRNAs differentially expressed in UC82 and Manduria, which led to an overall suitable level of tolerance to viral infection confirmed by the appearance of a recovery phenotype in Manduria and in all graft combinations. Read More

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February 2020

Potato virus Y; the Andean connection.

Virus Evol 2019 Jul 23;5(2):vez037. Epub 2019 Sep 23.

Laboratory of Plant Virology, Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, 1-banchi, Honjo-machi, Saga, Japan.

(PVY) causes disease in potatoes and other solanaceous crops. The appearance of its necrogenic strains in the 1980s made it the most economically important virus of potatoes. We report the isolation and genomic sequences of 32 Peruvian isolates of PVY which, together with 428 published PVY genomic sequences, gave an alignment of 460 sequences. Read More

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HCPro Suppression of Callose Deposition Contributes to Strain-Specific Resistance Against Potato Virus Y.

Phytopathology 2020 Jan 29;110(1):164-173. Epub 2019 Nov 29.

Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, U.S.A.

Potato virus Y (PVY; Potyviridae) is a continuing challenge for potato production owing to the increasing popularity of strain-specific resistant cultivars. Hypersensitive resistance (HR) is one type of plant defense responses to restrict virus spread. In many potato cultivars, such as cultivar Premier Russet (PR), local necrosis at the site of infection protects against the most common PVY strain, but the HR often fails to restrain necrotic strains, which spread systemically. Read More

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January 2020

Detection and Quantification of Genomes in Single Aphid Stylets.

M Khelifa

Plant Dis 2019 Sep 19;103(9):2315-2321. Epub 2019 Jul 19.

Fédération Nationale des Producteurs de Plants de Pomme de Terre/ Recherche, Développement et Promotion du Plant de Pomme de Terre (FN3PT/RD3PT), 75008 Paris, France.

Typically, the detection of a plant virus within its vector is carried out on the entire insect body. This process can be a possible source of confusion in the quantification of transmissible virus particles for styletborne viruses such as (PVY), since the transmissible virus fraction is the one only retained in the aphid vector's mouthparts. The objective of this study was to develop and validate the quantitative PCR method for the detection and quantification of PVY in the vector's stylet. Read More

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September 2019

Proliferation of Recombinant PVY Strains in Two Potato-Producing Regions of Canada, and Symptom Expression in 30 Important Potato Varieties with Different PVY Strains.

Plant Dis 2019 Sep 9;103(9):2221-2230. Epub 2019 Jul 9.

Agricultural Certification Services Inc., Fredericton, New Brunswick, E3B 8B7, Canada.

(PVY) exists as several strains with distinct symptomology and tuber yield effects in different potato varieties. Recently, new recombinant strains have proliferated and dominated local populations around the world. In this study, PVY, PVY, PVY, and PVY strains were tracked across Canada from 2014 to 2017, showing rapid evolution of populations away from the traditionally dominant PVY to recombinants PVY (western Canada) and PVY (eastern Canada). Read More

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September 2019

Complete genome characterization and population dynamics of potato virus Y-NTN strain from India.

Virusdisease 2019 Jun 5;30(2):252-260. Epub 2019 Apr 5.

1Department of Plant Pathology, Washington State University, Pullman, WA USA.

Potato virus Y (PVY) is a major threat to potato cultivation worldwide. PVY exists as biologically and genetically distinct strains and causes varying degrees of pathogenicity and a wide range of symptoms in potato. Knowledge of the nature of PVY strains is essential for breeding PVY resistant cultivars that are durable against a wide range of strains. Read More

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Characterization of potato virus Y populations in potato in Israel.

Arch Virol 2019 Jun 9;164(6):1691-1695. Epub 2019 Apr 9.

Department of Plant Pathology and Weed Science, Agricultural Research Organization-The Volcani Center, Rishon LeZion, 7505101, Israel.

Potato virus Y (PVY) is the most common virus infecting potato worldwide. We analysed potato tuber PVY infections from the major Israeli growing region in 2014-2017. Isolates were characterized by multiplex PCR according to Chikh-Ali et al. Read More

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Generation of virus-resistant potato plants by RNA genome targeting.

Plant Biotechnol J 2019 09 8;17(9):1814-1822. Epub 2019 Mar 8.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.

CRISPR/Cas systems provide bacteria and archaea with molecular immunity against invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13a is an RNA-targeting CRISPR effector that provides protection against RNA phages. Here we report the repurposing of CRISPR/Cas13a to protect potato plants from a eukaryotic virus, Potato virus Y (PVY). Read More

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September 2019

Genetic Diversity of Potato virus Y in Potato Production Areas in Northeast China.

Plant Dis 2019 Feb 3;103(2):289-297. Epub 2018 Dec 3.

Northeast Agricultural University, Harbin, Heilongjiang 150030, China.

In 2011-2014, ELISA or nucleic acid spot hybridization (NASH) testing for common potato viruses or Potato spindle tuber viroid (PSTVd) was performed on 500 leaf samples collected in potato fields in the northeast provinces Heilongjiang and Inner Mongolia, China. The results revealed that 38.4% (Heilongjiang) and 27. Read More

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February 2019

Anthropogenic influences on emergence of vector-borne plant viruses: the persistent problem of Potato virus Y.

Curr Opin Virol 2018 12 11;33:177-183. Epub 2018 Nov 11.

Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853, United States.

Potato virus Y (PVY) has reemerged as a serious impediment to seed potato production, responsible for reduced yields and tuber quality, as well as the majority of seed lot rejections by certification programs due to excessive virus incidence. This has led to seed shortages, especially in cultivars highly susceptible to infection. While seed certification programs have been effective at managing many virus diseases below economic thresholds, PVY has rapidly evolved in recent decades to become a complex of strains that evade many certification and farm management practices. Read More

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December 2018

microRNA response in potato virus Y infected tobacco shows strain-specificity depending on host and symptom severity.

Virus Res 2019 01 10;260:20-32. Epub 2018 Nov 10.

Plant Breeding and Acclimatization Institute - National Research Institute, Młochów Research Center, Platanowa 19, PL-05-831, Młochów, Poland.

The present study demonstrates how different potato virus Y (PVY) strains affect the miRNA balance in tobacco cv. Samsun. The two prevalent strains PVY and PVY caused severe and mild veinal necrosis (VN) respectively, and the unique PVY-NTN strain induced milder vein clearing (VCl) in the upper non-inoculated leaves. Read More

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January 2019

Species Composition of Alate Aphids (Hemiptera: Aphididae) Harboring Potato Virus Y and the Harbored Virus Strains in Hokkaido, Northern Japan.

J Econ Entomol 2019 02;112(1):85-90

Division of Agro-environmental Research, Hokkaido Agricultural Research Center, NARO, Hitsujigaoka 1, Toyohira-ku, Sapporo, Hokkaido, Japan.

Many studies have evaluated transmission abilities of laboratory-reared aphids for potato virus Y (PVY), but few have focused on PVY-harboring species of field-collected aphids and the strains of PVY harbored by aphids. In the present study, we collected alate aphids in yellow pan traps in potato fields with Japanese commercial cultivars in Hokkaido, northern Japan in single 24-h periods during the tuber bulking stage and examined whether individual whole aphids harbored PVY by nested RT-PCR. PVY-positive individuals were identified to species using the gene sequence for cytochrome c oxidase subunit I and, when needed, morphological data and distribution records. Read More

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February 2019

Integrating Spectroscopy with Potato Disease Management.

Plant Dis 2018 11 20;102(11):2233-2240. Epub 2018 Aug 20.

Department of Forest and Wildlife Ecology, University of Wisconsin-Madison.

Spectral phenotyping is an efficient method for the nondestructive characterization of plant biochemical and physiological status. We examined the ability of a full range (350 to 2,500 nm) of foliar spectral data to (i) detect Potato virus Y (PVY) and physiological effects of the disease in visually asymptomatic leaves, (ii) classify different strains of PVY, and (iii) identify specific potato cultivars. Across cultivars, foliar spectral profiles of PVY-infected leaves were statistically different (F = 96. Read More

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November 2018

High-degree and broad-spectrum resistance mediated by a combination of NIb siRNA and miRNA suppresses replication of necrotic and common strains of potato virus Y.

Arch Virol 2018 Nov 10;163(11):3073-3081. Epub 2018 Aug 10.

State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.

In plants, viral replication can be inhibited through gene silencing, which is mediated by short interfering RNA (siRNA) or microRNA (miRNA). However, under natural conditions, viruses are extremely susceptible to mutations that may decrease the efficiency of cleavage of these small RNAs (sRNAs). Therefore, a single sRNA may not provide a sufficient degree of viral resistance to transgenic plants. Read More

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November 2018

NtTPN1: a RPP8-like R gene required for Potato virus Y-induced veinal necrosis in tobacco.

Plant J 2018 Jun 4. Epub 2018 Jun 4.

UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, 71 Av. E. Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France.

Potato virus Y (PVY) is one of the most damaging viruses of tobacco. In particular, aggressive necrotic strains (PVY ) lead to considerable losses in yield. The main source of resistance against PVY is linked to the va locus. Read More

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Validated reverse transcription droplet digital PCR serves as a higher order method for absolute quantification of Potato virus Y strains.

Anal Bioanal Chem 2018 Jun 3;410(16):3815-3825. Epub 2018 May 3.

National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia.

RNA viruses have a great potential for high genetic variability and rapid evolution that is generated by mutation and recombination under selection pressure. This is also the case of Potato virus Y (PVY), which comprises a high diversity of different recombinant and non-recombinant strains. Consequently, it is hard to develop reverse transcription real-time quantitative PCR (RT-qPCR) with the same amplification efficiencies for all PVY strains which would enable their equilibrate quantification; this is specially needed in mixed infections and other studies of pathogenesis. Read More

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