242 results match your criteria pvy genome

biological strain group YD: hypersensitive resistance genes elicited and phylogenetic placement.

Plant Dis 2021 Jun 3. Epub 2021 Jun 3.

Fera Science Ltd, 16233, York, Yorkshire, United Kingdom of Great Britain and Northern Ireland;

Potato virus Y (PVY) disrupts healthy seed potato production and causes tuber yield and quality losses globally. Its subdivisions consist of strain groups defined by potato hypersensitive resistance (HR) genes and phylogroups defined by sequencing. When PVY isolate PP was inoculated to potato cultivar differentials with HR genes, the HR phenotype pattern obtained resembled that caused by strain group PVY isolate KIP1. Read More

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Molecular and biological characterization of Potato virus Y detected in zucchini in China.

Virusdisease 2021 Mar 18;32(1):117-122. Epub 2021 Mar 18.

College of Hortculture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010019 Inner Mongolia China.

Potato virus Y (PVY) is an important viral pathogen of the plant family . However, it has not been reported that PVY infects zucchini ( L). Zucchini plants showing symptoms of leaf mosaic and fruit ringspot were collected from Inner Mongolia, China. Read More

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Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains.

Plants (Basel) 2021 Apr 12;10(4). Epub 2021 Apr 12.

INRAE, University of Bordeaux, UMR BFP, 33140 Villenave d'Ornon, France.

In recent years, high throughput sequencing (HTS) has brought new possibilities to the study of the diversity and complexity of plant viromes. Mixed infection of a single plant with several viruses is frequently observed in such studies. We analyzed the virome of 10 tomato and sweet pepper samples from Slovakia, all showing the presence of potato virus Y (PVY) infection. Read More

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Genomic analysis of dibenzofuran-degrading Pseudomonas veronii strain Pvy reveals its biodegradative versatility.

G3 (Bethesda) 2021 Feb;11(2)

Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.

Certain industrial chemicals accumulate in the environment due to their recalcitrant properties. Bioremediation uses the capability of some environmental bacteria to break down these chemicals and attenuate the pollution. One such bacterial strain, designated Pvy, was isolated from sediment samples from a lagoon in Romania located near an oil refinery due to its capacity to degrade dibenzofuran (DF). Read More

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February 2021

Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus.

Arch Virol 2021 May 8;166(5):1427-1431. Epub 2021 Mar 8.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

Potato aucuba mosaic virus (PAMV), a positive single-strand RNA virus, has one of the longest genomes of the viruses in the genus Potexvirus. In 2019, potato samples with mottle and crinkling symptoms from Huzhou, Zhejiang province, China, were identified to be infected with PAMV, potato virus X (PVX), and potato virus Y (PVY) by transcriptome sequencing. To study the effects of single infection by PAMV, the full-length sequence of PAMV from Huzhou (MT193476) was determined and an infectious full-length cDNA clone was constructed. Read More

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Generation and Functional Analysis of Potato Virus Y mutants.

Bio Protoc 2020 Jul 20;10(14):e3692. Epub 2020 Jul 20.

Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.

Potato virus Y (PVY), the type member of the genus (family ), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to functional studies that explore the involvement of selected amino acids in different stages of the viral cycle. The only way to functionally challenge the role of particular amino acids in the coat protein of PVY, or in other viral proteins, is by using cDNA clones. Read More

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The evolutionary history and global spatio-temporal dynamics of potato virus Y.

Virus Evol 2020 Jul 21;6(2):veaa056. Epub 2020 Nov 21.

Laboratory of Plant Virology, Department of Biological Sciences, Faculty of Agriculture, Saga University, 1-banchi, Honjo-machi, Saga 840-8502, Japan.

Potato virus Y (PVY) is a destructive plant pathogen that causes considerable losses to global potato and tobacco production. Although the molecular structure of PVY is well characterized, the evolutionary and global transmission dynamics of this virus remain poorly understood. We investigated the phylodynamics of the virus by analysing 253 nucleotide sequences of the genes encoding the third protein (P3), cylindrical inclusion protein (CI), and the nuclear inclusion protein (NIb). Read More

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Potato Virus Y Emergence and Evolution from the Andes of South America to Become a Major Destructive Pathogen of Potato and Other Solanaceous Crops Worldwide.

Viruses 2020 12 12;12(12). Epub 2020 Dec 12.

The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.

The potato was introduced to Europe from the Andes of South America in the 16th century, and today it is grown worldwide; it is a nutritious staple food eaten by millions and underpins food security in many countries. Unknowingly, potato virus Y (PVY) was also introduced through trade in infected potato tubers, and it has become the most important viral pathogen of potato. Phylogenetic analysis has revealed the spread and emergence of strains of PVY, including strains causing economically important diseases in tobacco, tomato and pepper, and that the virus continues to evolve with the relatively recent emergence of new damaging recombinant strains. Read More

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December 2020

Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato.

Int J Mol Sci 2020 Dec 9;21(24). Epub 2020 Dec 9.

Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization ELGO-DIMITRA, Mesa Katsabas, 71307 Heraklion, Crete, Greece.

Defensins are small and rather ubiquitous cysteine-rich anti-microbial peptides. These proteins may act against pathogenic microorganisms either directly (by binding and disrupting membranes) or indirectly (as signaling molecules that participate in the organization of the cellular defense). Even though defensins are widespread across eukaryotes, still, extensive nucleotide and amino acid dissimilarities hamper the elucidation of their response to stimuli and mode of function. Read More

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December 2020

Molecular and biological characterization of recombinant isolates of circulating in potato fields in Mexico.

Plant Dis 2020 Dec 2. Epub 2020 Dec 2.

University of Idaho, EPPN, 875 Perimeter Dr., MS 2329, Moscow, Idaho, United States, 83844-2329;

(PVY) is a significant threat to potato production in Mexico. The presence of recombinant strains of PVY circulating in potato has been reported in the country, but no systematic study on the genetic diversity of PVY in potato and prevalence of PVY strains has been conducted yet. Here, we report on a series of surveys in seed potato production areas in two states in Mexico, Chihuahua and Jalisco, between 2011 and 2019. Read More

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December 2020

The Recombinant Potato virus Y (PVY) Strain, PVY, Identified in Potato Fields in Victoria, Southeastern Australia.

Plant Dis 2020 Dec 15;104(12):3110-3114. Epub 2020 Oct 15.

University of Idaho, Department of EPPN, Moscow, ID 83844-2329, U.S.A.

Potato virus Y (PVY) is one of the main viruses affecting potato in Australia. However, molecular characterization of PVY isolates circulating in potato in different states of Australia has not yet been thoroughly conducted. Only nonrecombinant isolates of three biological PVY strains collected from potato were reported previously from Western Australia and one from Queensland. Read More

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December 2020

Characterization of Potato Virus Y Isolates and Assessment of Nanopore Sequencing to Detect and Genotype Potato Viruses.

Viruses 2020 04 23;12(4). Epub 2020 Apr 23.

Crop Science Department, Teagasc, Oak Park, R93XE12 Carlow, Ireland.

Potato virus Y (PVY) is the most economically important virus infecting cultivated potato ( L.). Accurate diagnosis is crucial to regulate the trade of tubers and for the sanitary selection of plant material for propagation. Read More

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Natural resistance to Potato virus Y in Solanum tuberosum Group Phureja.

Theor Appl Genet 2020 Mar 16;133(3):967-980. Epub 2020 Jan 16.

The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.

Key Message: Novel major gene resistance against Potato virus Y in diploid populations of Solanum tuberosum Groups Phureja and Tuberosum was biologically and genetically characterised. Named Ry(o), it mapped to chromosome 9. A new source of genetic resistance derived from Solanum tuberosum Group Phureja against Potato virus Y (PVY) was identified and genetically characterised in three diploid biparental potato populations. Read More

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Overexpression of a modified eIF4E regulates potato virus Y resistance at the transcriptional level in potato.

BMC Genomics 2020 Jan 6;21(1):18. Epub 2020 Jan 6.

Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, 53706, USA.

Background: Potato virus Y (PVY) is a major pathogen of potatoes with major impact on global agricultural production. Resistance to PVY can be achieved by engineering potatoes to express a recessive, resistant allele of eukaryotic translation initiation factor eIF4E, a host dependency factor essential to PVY replication. Here we analyzed transcriptome changes in eIF4E over-expressing potatoes to shed light on the mechanism underpinning eIF4E-mediated recessive PVY resistance. Read More

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January 2020

Screening for functional IRESes using α-complementation system of β-galactosidase in .

Biotechnol Biofuels 2019 27;12:300. Epub 2019 Dec 27.

1Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Sciences, Fujian Normal University, Fuzhou, 350007 China.

Background: is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in are not efficient. The internal ribosome entry site (IRES) has an ability to recruit the ribosome to initiate protein synthesis by cap-independent translation manner. Read More

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December 2019

High-Throughput Sequencing Reveals Bell Pepper Endornavirus Infection in Pepper () in Slovakia and Enables Its Further Molecular Characterization.

Plants (Basel) 2019 Dec 26;9(1). Epub 2019 Dec 26.

Biomedical Research Center of the Slovak Academy of Sciences, Institute of Virology, Dúbravská cesta 9, 84505 Bratislava, Slovak Republic.

Ribosomal RNA-depleted total RNAs from a sweet pepper plant (, labelled as N65) grown in western Slovakia and showing severe virus-like symptoms (chlorosis, mottling and deformation of leaf lamina) were subjected to high-throughput sequencing (HTS) on an Illumina MiSeq platform. The de novo assembly of ca. 5. Read More

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December 2019

A functional investigation of the suppression of CpG and UpA dinucleotide frequencies in plant RNA virus genomes.

Sci Rep 2019 12 4;9(1):18359. Epub 2019 Dec 4.

Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, OX1 3SY, UK.

Frequencies of CpG and UpA dinucleotides in most plant RNA virus genomes show degrees of suppression comparable to those of vertebrate RNA viruses. While pathways that target CpG and UpAs in HIV-1 and echovirus 7 genomes and restrict their replication have been partly characterised, whether an analogous process drives dinucleotide underrepresentation in plant viruses remains undetermined. We examined replication phenotypes of compositionally modified mutants of potato virus Y (PVY) in which CpG or UpA frequencies were maximised in non-structural genes (including helicase and polymerase encoding domains) while retaining protein coding. Read More

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December 2019

Development and application of a full-length infectious clone of potato virus Y isolate belonging to SYR-I strain.

Virus Res 2020 01 27;276:197827. Epub 2019 Nov 27.

Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China; Shangdong Provincial Key Laboratory of Agricultural Microbiology, Tai'an, Shandong 271018, China. Electronic address:

Potato virus Y (PVY) causes huge damage to potato and tobacco production worldwide. The complete genome sequence of GZ, a PVY isolate (strain SYR-I) from Guizhou province, China, was cloned into the binary vector pCambia0390. Three introns were individually inserted into the P3 and CI ORFs to produce plasmid pCamPVY-GZ. Read More

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January 2020

Metagenomic analysis of virome cross-talk between cultivated Solanum lycopersicum and wild Solanum nigrum.

Virology 2020 01 8;540:38-44. Epub 2019 Nov 8.

UMR 1332 BFP, INRA, Univ. Bordeaux, CS20032, 33882, Villenave d'Ornon Cedex, France. Electronic address:

Wild plants and weeds growing close to crops constitute a potential reservoir for future epidemies or for the emergence of novel viruses but the frequency and directionality of viral flow between cultivated and wild plants remains poorly documented in many cases. Here, we studied the diversity of viral populations between tomato (Solanum lycopersicum) and neighboring european black nightshade (Solanum nigrum) using high throughput sequencing (HTS) based metagenomics. A large variability in virome richness with only 17. Read More

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January 2020

Genome-wide association mapping of QTLs implied in potato virus Y population sizes in pepper: evidence for widespread resistance QTL pyramiding.

Mol Plant Pathol 2020 01 11;21(1):3-16. Epub 2019 Oct 11.

Pathologie Végétale, INRA, 84140, Montfavet, France.

In this study, we looked for genetic factors in the pepper (Capsicum annuum) germplasm that control the number of potato virus Y (PVY) particles entering the plant (i.e. effective population size at inoculation) and the PVY accumulation at the systemic level (i. Read More

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January 2020

Molecular Evolutionary Analysis of Potato Virus Y Infecting Potato Based on the VPg Gene.

Front Microbiol 2019 26;10:1708. Epub 2019 Jul 26.

Heilongjiang Academy of Agricultural Sciences, Harbin, China.

Potato virus Y (PVY) is an important plant pathogen infecting solanaceous crops, causing significant losses to global potato and tobacco production. Some aspects of the plant pathology and molecular biology of PVY have been studied intensively, but the evolutionary dynamics of this virus are poorly understood. Here, we performed a comprehensive set of rigorous evolutionary analyses using 177 nucleotide sequences of the viral genome linked protein (VPg) gene, which interacts with the plant eukaryotic translation initiation factor 4E (eIF4E). Read More

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Extreme resistance to Potato virus Y in potato carrying the Ry gene is mediated by a TIR-NLR immune receptor.

Plant Biotechnol J 2020 03 4;18(3):655-667. Epub 2019 Sep 4.

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

Potato virus Y (PVY) is a major potato (Solanum tuberosum L.) pathogen that causes severe annual crop losses worth billions of dollars worldwide. PVY is transmitted by aphids, and successful control of virus transmission requires the extensive use of environmentally damaging insecticides to reduce vector populations. Read More

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Detection and Quantification of Genomes in Single Aphid Stylets.

M Khelifa

Plant Dis 2019 Sep 19;103(9):2315-2321. Epub 2019 Jul 19.

Fédération Nationale des Producteurs de Plants de Pomme de Terre/ Recherche, Développement et Promotion du Plant de Pomme de Terre (FN3PT/RD3PT), 75008 Paris, France.

Typically, the detection of a plant virus within its vector is carried out on the entire insect body. This process can be a possible source of confusion in the quantification of transmissible virus particles for styletborne viruses such as (PVY), since the transmissible virus fraction is the one only retained in the aphid vector's mouthparts. The objective of this study was to develop and validate the quantitative PCR method for the detection and quantification of PVY in the vector's stylet. Read More

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September 2019

Complete genome characterization and population dynamics of potato virus Y-NTN strain from India.

Virusdisease 2019 Jun 5;30(2):252-260. Epub 2019 Apr 5.

1Department of Plant Pathology, Washington State University, Pullman, WA USA.

Potato virus Y (PVY) is a major threat to potato cultivation worldwide. PVY exists as biologically and genetically distinct strains and causes varying degrees of pathogenicity and a wide range of symptoms in potato. Knowledge of the nature of PVY strains is essential for breeding PVY resistant cultivars that are durable against a wide range of strains. Read More

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CRISPR technology to combat plant RNA viruses: A theoretical model for Potato virus Y (PVY) resistance.

Microb Pathog 2019 Aug 22;133:103551. Epub 2019 May 22.

Department of Plant Pathology, Bahauddin Zakariya University, Multan, Pakistan.

RNA viruses are the most diverse phytopathogens which cause severe epidemics in important agricultural crops and threaten the global food security. Being obligatory intracellular pathogens, these viruses have developed fine-tuned evading mechanisms and are non-responsive to most of the prophylactic treatments. Additionally, their sprint ability to overcome host defense demands a broad-spectrum and durable mechanism of resistance. Read More

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A complex eIF4E locus impacts the durability of va resistance to Potato virus Y in tobacco.

Mol Plant Pathol 2019 08 21;20(8):1051-1066. Epub 2019 May 21.

UMR 1332 Biologie du Fruit et Pathologie, INRA, University Bordeaux, 71 Av. E. Bourlaux, Villenave d'Ornon Cedex CS 20032, 33882, France.

Many recessive resistances against potyviruses are mediated by eukaryotic translation initiation factor 4E (eIF4E). In tobacco, the va resistance gene commonly used to control Potato virus Y (PVY) corresponds to a large deletion affecting the eIF4E-1 gene on chromosome 21. Here, we compared the resistance durability conferred by various types of mutations affecting eIF4E-1 (deletions of various sizes, frameshift or nonsense mutations). Read More

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Immunocapture of virions with virus-specific antibodies prior to high-throughput sequencing effectively enriches for virus-specific sequences.

PLoS One 2019 9;14(5):e0216713. Epub 2019 May 9.

Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Plant Virus Department, Braunschweig, Germany.

Virus discovery based on high-throughput sequencing relies on enrichment for virus sequences prior to library preparation to achieve a sufficient number of viral reads. In general, preparations of double-stranded RNA or total RNA preparations treated to remove rRNA are used for sequence enrichment. We used virus-specific antibodies to immunocapture virions from plant sap to conduct cDNA synthesis, followed by library preparation and HTS. Read More

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February 2020

Characterization of potato virus Y populations in potato in Israel.

Arch Virol 2019 Jun 9;164(6):1691-1695. Epub 2019 Apr 9.

Department of Plant Pathology and Weed Science, Agricultural Research Organization-The Volcani Center, Rishon LeZion, 7505101, Israel.

Potato virus Y (PVY) is the most common virus infecting potato worldwide. We analysed potato tuber PVY infections from the major Israeli growing region in 2014-2017. Isolates were characterized by multiplex PCR according to Chikh-Ali et al. Read More

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Generation of virus-resistant potato plants by RNA genome targeting.

Plant Biotechnol J 2019 09 8;17(9):1814-1822. Epub 2019 Mar 8.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.

CRISPR/Cas systems provide bacteria and archaea with molecular immunity against invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13a is an RNA-targeting CRISPR effector that provides protection against RNA phages. Here we report the repurposing of CRISPR/Cas13a to protect potato plants from a eukaryotic virus, Potato virus Y (PVY). Read More

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September 2019

Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y O and N types in potato.

J Virol Methods 2019 05 20;267:16-21. Epub 2019 Feb 20.

Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, 53706, USA. Electronic address:

Potato virus Y (PVY) is a global challenge for potato production and the leading cause of seed crop downgrading and rejection for certification. Accurate and timely diagnosis is key to effective control of PVY. Here we optimized the isothermal recombinase polymerase amplification (RPA) for accurate detection of different PVY O and N types that were tested, present in different tissues of potato plants including tubers with a primer set that specifically targets the highly conserved pipo region within the viral genome. Read More

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