669 results match your criteria pvy


Extreme resistance to potato virus A in potato cultivar Barbara is independently mediated by Ra and Rysto.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, New Brunswick, Canada, E3B0E2;

Potato virus A (PVA) and potato virus Y (PVY) are two common members of Potyvirus genus infecting potato crops worldwide. Host resistance offers an economical and effective means for the control and/or management of these viruses. In this study, 20 potato clones were screened for their resistance against PVA and PVY by mechanical and/or graft inoculation assay, and were explored for the relationship between extreme resistance genes Ra and Ry by the detection of molecular markers linked respectively to Ryadg, Rysto, and Rychc. Read More

View Article and Full-Text PDF

biological strain group YD: hypersensitive resistance genes elicited and phylogenetic placement.

Plant Dis 2021 Jun 3. Epub 2021 Jun 3.

Fera Science Ltd, 16233, York, Yorkshire, United Kingdom of Great Britain and Northern Ireland;

Potato virus Y (PVY) disrupts healthy seed potato production and causes tuber yield and quality losses globally. Its subdivisions consist of strain groups defined by potato hypersensitive resistance (HR) genes and phylogroups defined by sequencing. When PVY isolate PP was inoculated to potato cultivar differentials with HR genes, the HR phenotype pattern obtained resembled that caused by strain group PVY isolate KIP1. Read More

View Article and Full-Text PDF

Differences in Virulence among PVY Isolates of Different Geographical Origins When Infecting an Experimental Host under Two Growing Environments Are Not Determined by HCPro.

Plants (Basel) 2021 May 28;10(6). Epub 2021 May 28.

Department of Microbial and Plant Biotechnology, Margarita Salas Center for Biological Research, Spanish Council for Scientific Research (CIB Margarita Salas-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.

The contribution of the HCPro factors expressed by several PVY isolates of different geographical origins (one from Scotland, one from Spain, and several from Tunisia) to differences in their virulence in plants was investigated under two growing conditions: standard (st; 26 °C and current ambient levels of CO), and climate change-associated (cc; 31 °C and elevated levels of CO). In all cases, relative infection symptoms and viral titers were determined. The viral cistrons were also sequenced and amino-acid features of the encoded proteins were established, as well as phylogenetic distances. Read More

View Article and Full-Text PDF

The Resistance Responses of Potato Plants to Potato Virus Y Are Associated with an Increased Cellular Methionine Content and an Altered SAM:SAH Methylation Index.

Viruses 2021 May 21;13(6). Epub 2021 May 21.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia.

Plant-virus interactions are frequently influenced by elevated temperature, which often increases susceptibility to a virus, a scenario described for potato cultivar Chicago infected with potato virus Y (PVY). In contrast, other potato cultivars such as Gala may have similar resistances to PVY at both normal (22 °C) and high (28 °C) temperatures. To elucidate the mechanisms of temperature-independent antivirus resistance in potato, we analysed responses of Gala plants to PVY at different temperatures using proteomic, transcriptional and metabolic approaches. Read More

View Article and Full-Text PDF

Purine Nucleoside Derivatives Containing a Sulfa Ethylamine Moiety: Design, Synthesis, Antiviral Activity, and Mechanism.

J Agric Food Chem 2021 May 14;69(20):5575-5582. Epub 2021 May 14.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, P. R. China.

To find efficient and broad-spectrum viral agents, a series of purine nucleoside derivatives containing sulfa ethylamine moieties was designed and synthesized, and their antiviral activities against tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and potato virus Y (PVY) were evaluated. Some target compounds displayed good antiviral activities. Among them, compound showed excellent protective activity against CMV and PVY with 50% effective concentration values (EC) of 137 and 209 μg/mL, respectively, which were better than that of the control agent ningnanmycin (508 and 431 μg/mL). Read More

View Article and Full-Text PDF

Molecular and biological characterization of Potato virus Y detected in zucchini in China.

Virusdisease 2021 Mar 18;32(1):117-122. Epub 2021 Mar 18.

College of Hortculture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010019 Inner Mongolia China.

Potato virus Y (PVY) is an important viral pathogen of the plant family . However, it has not been reported that PVY infects zucchini ( L). Zucchini plants showing symptoms of leaf mosaic and fruit ringspot were collected from Inner Mongolia, China. Read More

View Article and Full-Text PDF

Association mapping reveals novel genomic regions controlling some root and stolon traits in tetraploid potato ( L.).

3 Biotech 2021 Apr 18;11(4):174. Epub 2021 Mar 18.

Faculty of Agricultural Sciences and Technologies, Nigde Omer Halisdemir University, 51240 Nigde, Turkey.

Tuber crops have measurable biological variation in root and stolon phenotyping and thus may be utilized to identify genomic regions associated with these variations. This is the first comprehensive association mapping study related to potato root and stolon traits. A diverse panel of 192 tetraploid potato ( L. Read More

View Article and Full-Text PDF

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains.

Plants (Basel) 2021 Apr 12;10(4). Epub 2021 Apr 12.

INRAE, University of Bordeaux, UMR BFP, 33140 Villenave d'Ornon, France.

In recent years, high throughput sequencing (HTS) has brought new possibilities to the study of the diversity and complexity of plant viromes. Mixed infection of a single plant with several viruses is frequently observed in such studies. We analyzed the virome of 10 tomato and sweet pepper samples from Slovakia, all showing the presence of potato virus Y (PVY) infection. Read More

View Article and Full-Text PDF

Extreme Resistance to Viruses in Potato and Soybean.

Front Plant Sci 2021 6;12:658981. Epub 2021 Apr 6.

Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, United States.

Plant pathogens, including viruses, negatively impact global crop production. Plants have evolved complex immune responses to pathogens. These responses are often controlled by nucleotide-binding leucine-rich repeat proteins (NLRs), which recognize intracellular, pathogen-derived proteins. Read More

View Article and Full-Text PDF

The Structure of the Potato Virus A Particles Elucidated by Small Angle X-Ray Scattering and Complementary Techniques.

Biochemistry (Mosc) 2021 Feb;86(2):230-240

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, Moscow, 119333, Russia.

Potato virus A (PVA) protein coat contains on its surface partially unstructured N-terminal domain of the viral coat protein (CP), whose structural and functional characteristics are important for understanding the mechanism of plant infection with this virus. In this work, we investigated the properties and the structure of intact PVA and partially trypsinized PVAΔ32 virions using small-angle X-ray scattering (SAXS) and complimentary methods. It was shown that after the removal of 32 N-terminal amino acids of the CP, the virion did not disintegrate and remained compact, but the helical pitch of the CP packing changed. Read More

View Article and Full-Text PDF
February 2021

Occurrence and distribution of viruses infecting potato in Russia.

Lett Appl Microbiol 2021 Jul 4;73(1):64-72. Epub 2021 May 4.

Federal State Budget Scientific Institution, All-Russian Institute of Plant Protection (FSBSI VIZR), Saint Petersburg, Russia.

Potato viral disease has been a major problem in potato production worldwide including Russia. Here, we detected Potato Virus M (PVM), P (PVP), S (PVS), Y (PVY), and X (PVX) and Potato Leaf Roll Virus (PLRV) by RT-PCR on potato leaves and tubers from the Northwestern (NW), Volga (VF), and Far Eastern (FE) federal districts of Russia. Each sample was co-infected with up to five viruses. Read More

View Article and Full-Text PDF

Topical Application of -Encapsulated dsRNA Induces Resistance in to Potato Viruses and Involves RDR6 and Combined Activities of DCL2 and DCL4.

Plants (Basel) 2021 Mar 29;10(4). Epub 2021 Mar 29.

Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.

Exogenous application of double-stranded RNAs (dsRNAs) for inducing virus resistance in plants represents an attractive alternative to transgene-based silencing approaches. However, improvement of dsRNA stability in natural conditions is required in order to provide long-term protection against the targeted virus. Here, we tested the protective effect of topical application of -encapsulated dsRNA compared to naked dsRNA against single and dual infection by expressing the green fluorescent protein (PVX-GFP) and Potato virus Y (PVY) in . Read More

View Article and Full-Text PDF

Different potato virus Y strains frequently co-localize in single epidermal leaf cells and in the aphid stylet.

J Gen Virol 2021 Mar 12;102(3). Epub 2021 Mar 12.

USDA-ARS, Emerging Pests and Pathogen Research Unit, Ithaca, NY 14853-5904, USA.

Single aphids can simultaneously or sequentially acquire and transmit multiple potato virus Y (PVY) strains. Multiple PVY strains are often found in the same field and occasionally within the same plant, but little is known about how PVY strains interact in plants or in aphid stylets. Immuno-staining and confocal microscopy were used to examine the spatial and temporal dynamics of PVY strain mixtures (PVY and PVY or PVY and PVY) in epidermal leaf cells of 'Samsun NN' tobacco and 'Goldrush' potato. Read More

View Article and Full-Text PDF

Genomic analysis of dibenzofuran-degrading Pseudomonas veronii strain Pvy reveals its biodegradative versatility.

G3 (Bethesda) 2021 Feb;11(2)

Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.

Certain industrial chemicals accumulate in the environment due to their recalcitrant properties. Bioremediation uses the capability of some environmental bacteria to break down these chemicals and attenuate the pollution. One such bacterial strain, designated Pvy, was isolated from sediment samples from a lagoon in Romania located near an oil refinery due to its capacity to degrade dibenzofuran (DF). Read More

View Article and Full-Text PDF
February 2021

Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus.

Arch Virol 2021 May 8;166(5):1427-1431. Epub 2021 Mar 8.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

Potato aucuba mosaic virus (PAMV), a positive single-strand RNA virus, has one of the longest genomes of the viruses in the genus Potexvirus. In 2019, potato samples with mottle and crinkling symptoms from Huzhou, Zhejiang province, China, were identified to be infected with PAMV, potato virus X (PVX), and potato virus Y (PVY) by transcriptome sequencing. To study the effects of single infection by PAMV, the full-length sequence of PAMV from Huzhou (MT193476) was determined and an infectious full-length cDNA clone was constructed. Read More

View Article and Full-Text PDF

StPIP1, a PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with Potato virus Y.

J Exp Bot 2021 May;72(12):4472-4488

Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA.

The role of small secreted peptides in plant defense responses to viruses has seldom been investigated. Here, we report a role for potato (Solanum tuberosum) PIP1, a gene predicted to encode a member of the pathogen-associated molecular pattern (PAMP)-induced peptide (PIP) family, in the response of potato to Potato virus Y (PVY) infection. We show that exogenous application of synthetic StPIP1 to potato leaves and nodes increased the production of reactive oxygen species and the expression of plant defense-related genes, revealing that StPIP1 triggers early defense responses. Read More

View Article and Full-Text PDF

Generation and Functional Analysis of Potato Virus Y mutants.

Bio Protoc 2020 Jul 20;10(14):e3692. Epub 2020 Jul 20.

Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.

Potato virus Y (PVY), the type member of the genus (family ), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to functional studies that explore the involvement of selected amino acids in different stages of the viral cycle. The only way to functionally challenge the role of particular amino acids in the coat protein of PVY, or in other viral proteins, is by using cDNA clones. Read More

View Article and Full-Text PDF

Increased ribonuclease activity in Solanum tuberosum L. transformed with heterologous genes of apoplastic ribonucleases as a putative approach for production of virus resistant plants.

Turk J Biol 2021 9;45(1):79-87. Epub 2021 Feb 9.

Institute of Cell Biology and Genetic Engineering NAS Ukraine, Kyiv Ukraine.

Viral pathogens seriously decrease the yield of potato ( L.) -an important agricultural crop. Therefore, there is a demand for potato cultivars resistant to multiple viruses. Read More

View Article and Full-Text PDF
February 2021

The Gene Can Regulate the Fecundity of the Green Peach Aphid, (Sulzer).

Front Physiol 2020 12;11:596392. Epub 2021 Jan 12.

College of Plant Protection, Hunan Agricultural University, Changsha, China.

(Sulzer), commonly known as the green peach aphid, is a notorious pest that causes substantial losses to a range of crops and can transmit several plant viruses, including potato virus Y (PVY). Chemical insecticides provide only partial control of this pest and their use is not environmentally sustainable. In recent years, many genes related to growth, development, and reproduction have been used as targets for pest control. Read More

View Article and Full-Text PDF
January 2021

Interspecific interactions within a vector-borne complex are influenced by a co-occurring pathosystem.

Sci Rep 2021 Jan 26;11(1):2242. Epub 2021 Jan 26.

Department of Entomology, Plant Pathology and Nematology, University of Idaho, Aberdeen R&E Center, Aberdeen, ID, 83210, USA.

Potato virus Y (PVY) and zebra chip (ZC) disease are major threats to solanaceous crop production in North America. PVY can be spread by aphid vectors and through vegetative propagation in potatoes. ZC is associated with "Candidatus Liberibacter solanacearum" (Lso), which is transmitted by the tomato/potato psyllid, Bactericera cockerelli Šulc (Hemiptera: Triozidae). Read More

View Article and Full-Text PDF
January 2021

Artificially Edited Alleles of the Eukaryotic Translation Initiation Factor 4E1 Gene Differentially Reduce Susceptibility to Cucumber Mosaic Virus and Potato Virus Y in Tomato.

Front Microbiol 2020 10;11:564310. Epub 2020 Dec 10.

Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.

Eukaryotic translation initiation factors, including eIF4E, are susceptibility factors for viral infection in host plants. Mutation and double-stranded RNA-mediated silencing of tomato genes can confer resistance to viruses, particularly members of the genus. Here, we artificially mutated the gene on chromosome 3 of a commercial cultivar of tomato ( L. Read More

View Article and Full-Text PDF
December 2020

Reference gene selection for miRNA and mRNA normalization in potato in response to potato virus Y.

Mol Cell Probes 2021 02 25;55:101691. Epub 2020 Dec 25.

Plant Breeding and Acclimatization Institute, National Research Institute, Młochów Research Center, Platanowa 19, Młochów, PL-05-831, Poland.

This was the first report on evaluating candidate reference genes for quantifying the expression profiles of both coding (e.g., mRNA) and non-coding (e. Read More

View Article and Full-Text PDF
February 2021

The evolutionary history and global spatio-temporal dynamics of potato virus Y.

Virus Evol 2020 Jul 21;6(2):veaa056. Epub 2020 Nov 21.

Laboratory of Plant Virology, Department of Biological Sciences, Faculty of Agriculture, Saga University, 1-banchi, Honjo-machi, Saga 840-8502, Japan.

Potato virus Y (PVY) is a destructive plant pathogen that causes considerable losses to global potato and tobacco production. Although the molecular structure of PVY is well characterized, the evolutionary and global transmission dynamics of this virus remain poorly understood. We investigated the phylodynamics of the virus by analysing 253 nucleotide sequences of the genes encoding the third protein (P3), cylindrical inclusion protein (CI), and the nuclear inclusion protein (NIb). Read More

View Article and Full-Text PDF

Potato Virus Y Emergence and Evolution from the Andes of South America to Become a Major Destructive Pathogen of Potato and Other Solanaceous Crops Worldwide.

Viruses 2020 12 12;12(12). Epub 2020 Dec 12.

The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.

The potato was introduced to Europe from the Andes of South America in the 16th century, and today it is grown worldwide; it is a nutritious staple food eaten by millions and underpins food security in many countries. Unknowingly, potato virus Y (PVY) was also introduced through trade in infected potato tubers, and it has become the most important viral pathogen of potato. Phylogenetic analysis has revealed the spread and emergence of strains of PVY, including strains causing economically important diseases in tobacco, tomato and pepper, and that the virus continues to evolve with the relatively recent emergence of new damaging recombinant strains. Read More

View Article and Full-Text PDF
December 2020

Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato.

Int J Mol Sci 2020 Dec 9;21(24). Epub 2020 Dec 9.

Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization ELGO-DIMITRA, Mesa Katsabas, 71307 Heraklion, Crete, Greece.

Defensins are small and rather ubiquitous cysteine-rich anti-microbial peptides. These proteins may act against pathogenic microorganisms either directly (by binding and disrupting membranes) or indirectly (as signaling molecules that participate in the organization of the cellular defense). Even though defensins are widespread across eukaryotes, still, extensive nucleotide and amino acid dissimilarities hamper the elucidation of their response to stimuli and mode of function. Read More

View Article and Full-Text PDF
December 2020

Molecular and biological characterization of recombinant isolates of circulating in potato fields in Mexico.

Plant Dis 2020 Dec 2. Epub 2020 Dec 2.

University of Idaho, EPPN, 875 Perimeter Dr., MS 2329, Moscow, Idaho, United States, 83844-2329;

(PVY) is a significant threat to potato production in Mexico. The presence of recombinant strains of PVY circulating in potato has been reported in the country, but no systematic study on the genetic diversity of PVY in potato and prevalence of PVY strains has been conducted yet. Here, we report on a series of surveys in seed potato production areas in two states in Mexico, Chihuahua and Jalisco, between 2011 and 2019. Read More

View Article and Full-Text PDF
December 2020

Current-Season Infection With Ordinary and Recombinant Strains of Has Detrimental Effects on Chipping Potato Yield and Tuber Quality.

Plant Dis 2021 Feb 18;105(2):450-455. Epub 2020 Nov 18.

Department of Horticulture, University of Wisconsin, Madison, WI.

(PVY) is the most economically important virus infecting potatoes worldwide. Current-season spread of PVY occurs when aphids transmit the virus from infected to noninfected plants during the growing season. The impact of current-season PVY infection on yield and quality of chip processing potatoes is not well documented. Read More

View Article and Full-Text PDF
February 2021

Potato Virus A Isolates from Three Continents: Their Biological Properties, Phylogenetics, and Prehistory.

Phytopathology 2021 Jan 15;111(1):217-226. Epub 2020 Dec 15.

Institute of Agriculture, University of Western Australia, Crawley, WA, Australia.

Forty-seven potato virus A (PVA) isolates from Europe, Australia, and South America's Andean region were subjected to high-throughput sequencing, and 46 complete genomes from Europe ( = 9), Australia ( = 2), and the Andes ( = 35) obtained. These and 17 other genomes gave alignments of 63 open reading frames 9,180 nucleotides long; 9 were recombinants. The nonrecombinants formed three tightly clustered, almost equidistant phylogroups; A comprised 14 Peruvian potato isolates; W comprised 37 from potato in Peru, Argentina, and elsewhere in the world; and T contained three from tamarillo in New Zealand. Read More

View Article and Full-Text PDF
January 2021

Role of the methionine cycle in the temperature-sensitive responses of potato plants to potato virus Y.

Mol Plant Pathol 2021 01 4;22(1):77-91. Epub 2020 Nov 4.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.

Plant-virus interactions are greatly influenced by environmental factors such as temperatures. In virus-infected plants, enhanced temperature is frequently associated with more severe symptoms and higher virus content. However, the mechanisms involved in such regulatory effects remain largely uncharacterized. Read More

View Article and Full-Text PDF
January 2021

Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis.

Plants (Basel) 2020 Oct 22;9(11). Epub 2020 Oct 22.

State Key Laboratory for Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management, MOA, China Agricultural University, Beijing 100193, China.

Potato () is a major food source in the whole world including Bangladesh. Viral diseases are the key constraint for sustainable potato production by reducing both quality and quantity. To determine the present status of eight important potato viruses in Bangladesh, tuber samples were collected from three major potato growing regions (Munshiganj, Jessore and Bogra districts) in January-February 2017 and February 2018. Read More

View Article and Full-Text PDF
October 2020