12,831 results match your criteria purification deae-cellulose


Bioprocess development for enhanced endoglucanase production by newly isolated bacteria, purification, characterization and in-vitro efficacy as anti-biofilm of Pseudomonas aeruginosa.

Sci Rep 2021 May 7;11(1):9754. Epub 2021 May 7.

Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Menoufia Governorate, 22857, Egypt.

Endoglucanase producing bacteria were isolated from Egyptian soils and the most active bacterial strain was identified as Bacillus subtilis strain Fatma/1. Plackett-Burman statistical design was carried out to assess the effect of seven process variables on endoglucanase production. Carboxymethyl cellulose (CMC), yeast extract and peptone were the most significant variables that enhanced the endoglucanase production and thus were selected for further optimization using face-centered central composite design. Read More

View Article and Full-Text PDF

Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed.

Int J Biol Macromol 2021 Mar 18;180:643-653. Epub 2021 Mar 18.

Department of Food Science and Technology, Faculty of Agriculture Engineering, Shahrood University of Technology, P.O. Box 361999-5161, Shahrood, Iran.

In this study, a novel polysaccharide fraction from Ocimum album seed was extracted and then purified by Cellulose DEAE-52 and Sephadex G-200 anion exchange chromatography. The structural, physicochemical and antioxidant properties of the main polysaccharide fraction (OAP-1A) were evaluated. The purified polysaccharide contained 94. Read More

View Article and Full-Text PDF

Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.

BMC Chem 2021 Mar 17;15(1):19. Epub 2021 Mar 17.

School of Pharmacy, Wannan Medical College, Wuhu, People's Republic of China.

Background: Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure. These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.

Results: This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs). Read More

View Article and Full-Text PDF

Purification, structural characterization, and biological activities of degraded polysaccharides from Porphyra yezoensis.

J Food Biochem 2021 Apr 17;45(4):e13661. Epub 2021 Feb 17.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, P.R. China.

The degraded polysaccharides from Porphyra yezoensis (DPPY) prepared using the H O -Vc method under optimized conditions were isolated and purified by DEAE Cellulose-52, and Sephadex G-100, providing four pure components, namely, DPPY-0, DPPY-0.1, DPPY-0.3, and DPPY-0. Read More

View Article and Full-Text PDF

Optimization of Extraction and Purification of Polysaccharides from Veronicastrum axillare, and Evaluation of Their Biological Activities.

Chem Biodivers 2021 Mar 24;18(3):e2000864. Epub 2021 Feb 24.

College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, P. R. China.

Veronicastrum axillare polysaccharides (VAP) were isolated by cellulase-assisted digestion. The optimum conditions (2 % cellulase, 47 °C for 2.5 h, then, 95 °C for 2. Read More

View Article and Full-Text PDF

Purification and characterization of a novel protein with activity against non-small-cell lung cancer in vitro and in vivo from the edible mushroom Boletus edulis.

Int J Biol Macromol 2021 Mar 27;174:77-88. Epub 2021 Jan 27.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. Electronic address:

A new anti-tumor protein (designated as Boletus edulis or in short BEAP) was isolated from dried fruit bodies of the edible bolete mushroom Boletus edulis. The purification protocol employed comprised fast ion exchange chromatography on a Hitrap Q column and ion exchange chromatography on a DEAE-52 cellulose column. Superdex G75 gel filtration and SDS-PAGE analysis revealed that BEAP was a protein with a molecular weight of 16. Read More

View Article and Full-Text PDF

Study on a novel spherical polysaccharide from Fructus Mori with good antioxidant activity.

Carbohydr Polym 2021 Mar 16;256:117516. Epub 2020 Dec 16.

School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China; SCUT-Zhuhai Institute of Modern Industrial Innovation, Zhuhai, 519715, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, 510640, China. Electronic address:

A novel polysaccharide (MFP1P) was isolated from Fructus Mori, followed by purification via DEAE-52 cellulose and 27 % ethanol fraction. The MFP1P had the molecular weight of 56.78 kDa and the total sugar content of 93. Read More

View Article and Full-Text PDF

Phospholipase A2 enzyme from the venom of Egyptian honey bee Apis mellifera lamarckii with anti-platelet aggregation and anti-coagulation activities.

J Genet Eng Biotechnol 2021 Jan 14;19(1):10. Epub 2021 Jan 14.

Molecular Biology Department, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt.

Background: Honey bee venom contains various enzymes with wide medical and pharmaceutical applications.

Results: The phospholipase A2 (PLA2) has been apparently purified from the venom of Egyptian honey bee (Apis mellifera lamarckii) 8.9-fold to a very high specific activity of 6033 U/mg protein using DEAE-cellulose and Sephacryl S-300 columns. Read More

View Article and Full-Text PDF
January 2021

Okra pectin relieves inflammatory response and protects damaged intestinal barrier in caerulein-induced acute pancreatic model.

J Sci Food Agric 2021 Feb 27;101(3):863-870. Epub 2020 Aug 27.

Engineering Research Center of Bio-Process of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Background: Protecting the intestinal mucosa from being destroyed helps reduce the inflammation caused by acute pancreatitis (AP). In this study, whether okra pectin (OP) could attenuate the inflammation of AP through protecting the intestinal barrier was investigated.

Results: OP was obtained from crude okra pectin (COP) through the purification by DEAE cellulose 52 column. Read More

View Article and Full-Text PDF
February 2021

A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity Purified from the Field Blewit Mushroom Lepista personata (Agaricomycetes).

Int J Med Mushrooms 2020 ;22(10):991-1000

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

A ribonuclease was purified from dry fruiting bodies of the wild edible mushroom Lepista personata (LPR) to 259-fold with a specific activity of 280 U/mg. The purification protocol involved ion-exchange chromatography on DEAE-cellulose, CM-cellulose and SP-sepharose, followed by size exclusion chromatography on Superdex 75. LPR is a homodimeric protein with a molecular weight of 27. Read More

View Article and Full-Text PDF
January 2020

Purification, characterization and partial amino acid sequences of thermo-alkali-stable and mercury ion-tolerant xylanase from Thermomyces dupontii KKU-CLD-E2-3.

Sci Rep 2020 12 10;10(1):21663. Epub 2020 Dec 10.

Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.

We investigated the properties of the low molecular weight thermo-alkali-stable and mercury ion-tolerant xylanase production from Thermomyces dupontii KKU-CLD-E2-3. The xylanase was purified to homogeneity by ammonium sulfate, Sephadex G-100 and DEAE-cellulose column chromatography which resulted 27.92-fold purification specific activity of 56. Read More

View Article and Full-Text PDF
December 2020

Purification and characterization of a noble thermostable algal starch liquefying alpha-amylase from BTPS-2 isolated from geothermal spring of Nepal.

Biotechnol Rep (Amst) 2020 Dec 1;28:e00551. Epub 2020 Nov 1.

Department of Biotechnology, Kathmandu University, Dhulikhel, 6250, Nepal.

A thermophilic strain, BTPS-2 was isolated from Bhurung geothermal spring of Nepal. The 16 s rRNA sequence showed 99.8 % similarity with the type strain DSM 3670. Read More

View Article and Full-Text PDF
December 2020

Purification and stability analysis of antimicrobial proteins from Varuna litterata.

Microb Pathog 2021 Apr 21;153:104637. Epub 2020 Nov 21.

Department of Biology & Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, College of Science, Shantou University, Shantou, Guangdong, 515063, PR China. Electronic address:

Ten marine species, including different crabs, bivalve molluscs, and fish intestines were selected to screen the natural antimicrobial protein or peptide as they are enriched with various microorganisms. The crude extract from Varuna litterata, a marine crab which is used as a raw material in the preparation of pickled crabs in Chaoshan area of China, was proved to have a potent bacteriostatic effect against gram-negative bacterium (Escherichia coli) and gram-positive bacterium(Staphylococcus aureus) compared with other marine species. The crude proteins of Varunalitterata were salted-out for preliminary purification and further purified by gel filtration (Sephadex G-150) or anion exchange (DEAE-cellulose 52) chromatographic column. Read More

View Article and Full-Text PDF

A halotolerant hyaluronidase from newly isolated Brevibacterium halotolerans DC1: Purification and characterization.

Int J Biol Macromol 2021 Jan 2;166:839-850. Epub 2020 Nov 2.

Department of Microbiology, School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon 425 001, India. Electronic address:

An enzyme hyaluronidase (hyase) producing halotolerant bacterium was isolated from dental caries and identified as Brevibacterium halotolerans DC1. Higher growth and hyase production were observed in nutrient broth fortified with hyaluronic acid at pH 7.0, temperature 37 °C, 120 rpm upon 48 h of incubation. Read More

View Article and Full-Text PDF
January 2021

Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.

Carbohydr Polym 2021 Jan 11;251:117078. Epub 2020 Sep 11.

Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, Heilongjiang, China. Electronic address:

The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. Read More

View Article and Full-Text PDF
January 2021

Purification and characterization of thermostable α-amylase produced from Bacillus licheniformis So-B3 and its potential in hydrolyzing raw starch.

Life Sci 2021 Jan 24;264:118639. Epub 2020 Oct 24.

Sen Research Group, Department of Biochemistry, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100 Kütahya, Turkey.

Aims: This work was achieved to obtain the optimum culture conditions of the thermostable alpha-amylase produced by thermophilic Bacillus licheniformis SO-B3. Furthermore, the α-amylase was purified and then characterized, and also its kinetic parameters were determined.

Materials And Methods: A new thermotolerant bacteria called Bacillus licheniformis SO-B3 employed in this work was isolated from a sample of thermal spring mud in Şırnak (Meyremderesi). Read More

View Article and Full-Text PDF
January 2021

Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from .

Evid Based Complement Alternat Med 2020 17;2020:5175138. Epub 2020 Oct 17.

Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.

Firstly, optimal parameters of crude polysaccharide from were obtained as follows: ratio of water to raw material of 26 : 1, ultrasonic power of 240 W, ultrasonic time of 45 min, and ultrasonic temperature of 62°C. Secondly, acidic polysaccharide (APBOM) from was successfully acquired with the yield of 9.57% by using DEAE-52 cellulose and Sephadex G-100 gel column chromatography. Read More

View Article and Full-Text PDF
October 2020

Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.

Int J Biol Macromol 2020 Dec 16;165(Pt B):2116-2125. Epub 2020 Oct 16.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, Zhejiang 310018, PR China. Electronic address:

In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography. Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed. The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264. Read More

View Article and Full-Text PDF
December 2020

Investigating the thermodynamic and kinetics properties of acid phosphatase extracted and purified from seedlings of Chenopodium murale.

Int J Biol Macromol 2020 Dec 13;165(Pt A):1475-1481. Epub 2020 Oct 13.

National Center of Excellence in Physical Chemistry, University of Peshawar, 25120, Pakistan.

Herein acid phosphatase isoenzyme was extracted from the C. murale seedlings. The purification was accomplished by chromatographic techniques and passing through DEAE-cellulose and Sephadex G-100 column. Read More

View Article and Full-Text PDF
December 2020

Extraction optimization, physicochemical properties and antioxidant and hypoglycemic activities of polysaccharides from roxburgh rose (Rosa roxburghii Tratt.) leaves.

Int J Biol Macromol 2020 Dec 28;165(Pt A):517-529. Epub 2020 Sep 28.

College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial Key Lab of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address:

The optimum extraction conditions of polysaccharides from roxburgh rose (Rosa roxburghii Tratt.) leaves (RLP) were obtained by response surface methodology (RSM), which were a liquid to solid ratio of 21.16 mL/g, an extraction temperature of 81. Read More

View Article and Full-Text PDF
December 2020

Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by .

Food Sci Nutr 2020 Sep 19;8(9):4957-4964. Epub 2020 Jul 19.

Department of Food Science and Technology Faculty of Agriculture Shahrood University of Technology Shahrood Iran.

Several strains of microorganism are capable of converting carbohydrates into extracellular polysaccharide. The preset research is a first effort made to optimize extracellular polysaccharide (CRMEP) by ATCC 10658 using one factor at time and response surface methods. One factor at time was applied in the initial screening of substrates prior to optimization study. Read More

View Article and Full-Text PDF
September 2020

Immunomodulatory and Antioxidant Effects of Polysaccharides from the Parasitic Fungus .

Biomed Res Int 2020 29;2020:8257847. Epub 2020 Aug 29.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.

The ascomycete Cordyceps genus has been used as valued traditional Chinese medicine. is a unique species of Cordyceps, which parasitizes on the larvae of Walker, and its major component cordycepin and aqueous extract are known to have many pharmacological effects. However, the physiological function of water-soluble polysaccharides has not been explored in detail. Read More

View Article and Full-Text PDF

Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body.

Int J Biol Macromol 2020 Dec 10;164:1879-1888. Epub 2020 Aug 10.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address:

The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order. Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps. Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR). Read More

View Article and Full-Text PDF
December 2020

Optimization, characterization, rheological study and immune activities of polysaccharide from Sagittaria sagittifolia L.

Carbohydr Polym 2020 Oct 11;246:116595. Epub 2020 Jun 11.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China. Electronic address:

To improve the extraction efficiency of polysaccharides from Sagittaria sagittifolia L. (SPU) by ultrasonic assisted extraction (UAE), the optimal extraction conditions were optimized as follows: extraction temperature of 85℃, extraction time of 15 min and ratio of liquid to raw material 43 mL/g, under these conditions, the yield of SPU increased by about 168 % compared with hot water extraction (HWE). After separation and purification by DEAE-52 cellulose column and Sephadex G-50 column, the pure polysaccharide fraction (SPU70-W1) was obtained, and its structure, rheology and immune activity were analyzed. Read More

View Article and Full-Text PDF
October 2020

Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.

Food Funct 2020 Aug 3;11(8):7104-7114. Epub 2020 Aug 3.

Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, Jiangxi, PR China.

A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification. The average molecular weight of PLP-1 was 16.2 kDa. Read More

View Article and Full-Text PDF

Immunomodulatory effect of polysaccharides from the extraction of (Blume) Hook. f. et Thomson (Campanulaceae) roots in female rats.

Nat Prod Res 2020 Aug 3:1-5. Epub 2020 Aug 3.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medcial University, Guiyang, PR China.

In this study, the crude polysaccharides of (CJP) was obtained from (Blume) Hook. f. et Thomson using hot water extraction method, which was separated and purified by DEAE-cellulose column and Sepharose CL-6B column. Read More

View Article and Full-Text PDF

A Glucose binding lectin from Leucaena leucocephala seeds and its mitogenic activity against human lymphocytes.

Int J Biol Macromol 2020 Nov 6;163:431-441. Epub 2020 Jul 6.

Department of Biotechnology, University of Calicut, Kerala 673635, India. Electronic address:

Lectins are a specialized group of proteins with immense biological properties and applications. This study describes the purification and characterization of a lectin from Leucaena leucocephala seeds, a plant belonging to the Fabaceae family. Leucaena leucocephala lectin (LLL) was purified by a two-step purification method involving DEAE-cellulose anion exchange chromatography and Sephadex G-75 size exclusion chromatography. Read More

View Article and Full-Text PDF
November 2020

A new water-soluble polysaccharide from Echinops pungens Trautv roots. Part I. Isolation, purification, characterization and antioxidant activity.

Int J Biol Macromol 2020 Oct 15;161:909-916. Epub 2020 Jun 15.

Shahrood University of Technology, Faculty of Agriculture, Department of Food Science and Technology, Shahrood, Iran. Electronic address:

EPPS, a new heteropolysaccharide from Echinops pungens roots, was extracted by warm water (70 °C) and fortuitously purified using column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Total carbohydrate content, specific optical rotation and molecular mass of EPPS were 98.3%, -88. Read More

View Article and Full-Text PDF
October 2020

Isolation, structure identification and anti-inflammatory activity of a polysaccharide from Phragmites rhizoma.

Int J Biol Macromol 2020 Oct 15;161:810-817. Epub 2020 Jun 15.

Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai 201306, China. Electronic address:

Phragmites rhizoma (PR) is comprised of polysaccharides as its main active component. Currently, there are few studies on polysaccharides from PR, especially in purification and structure. In this study, an acidic polysaccharide (PRP-2) was obtained from PR by ultrasonic assisted extraction and secondary column chromatography purification (Diethylaminoethyl cellulose-52 (DEAE-52) and Sephadex G-100). Read More

View Article and Full-Text PDF
October 2020

Structural characterization and immunomodulatory activity of a polysaccharide from Eurotium cristatum.

Int J Biol Macromol 2020 Nov 13;162:609-617. Epub 2020 Jun 13.

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Biotech & Biomed Research Institute, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China. Electronic address:

By combining DEAE-cellulose-52 and Sepharose-CL-6B chromatography, a heteropolysaccharide ECIP-1A with an average molecular weight of 24,336 Da was isolated from the hyphae of Eurotium cristatum. The main components of the monosaccharides of ECIP-1A were mannose, glucose and galactose, and their molar ratio is 4.003: 0. Read More

View Article and Full-Text PDF
November 2020