8,277 results match your criteria pure amino


Up-regulation of ribosomal and carbon metabolism proteins enhanced pyrene biodegradation in fulvic acid-induced biofilm system.

Environ Pollut 2021 Nov 29;294:118602. Epub 2021 Nov 29.

Center for Analysis and Testing, School of Chemistry and Materials, Nanjing Normal University, Nanjing, 210023, China. Electronic address:

The polycyclic aromatic hydrocarbons (PAHs) that enter the aqueous phase usually coexist with fulvic acid (FA). Therefore, we initiated this investigation to explore the influences of FA on bacterial biofilm formation and its potential to biodegrade pyrene (PYR), using electron microscopic techniques and isobaric tags for relative and absolute quantification (iTRAQ). Our results revealed that FA stimulated biofilm formation and enhanced the biodegradation of PYR. Read More

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November 2021

Nano-Interlayers Fabricated via Interfacial Azo-Coupling Polymerization: Effect of Pore Properties of Interlayers on Overall Performance of Thin-Film Composite for Nanofiltration.

ACS Appl Mater Interfaces 2021 Dec 2. Epub 2021 Dec 2.

Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

The supporting layer of nanofiltration membranes is critical to the overall nanofiltration performance. However, conventional supports lack efficient surface porosity, which leads to the limited utilization rate of the polyamide (PA) layer. Herein a double-skin-layer nanofiltration membrane with porous organic polymer nanointerlayers prepared via a two-step interfacial polymerization technique is presented to investigate the effect of the interlayers' pore properties on the performance of the thin-film composite. Read More

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December 2021

Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride.

R Soc Open Sci 2021 Nov 24;8(11):211027. Epub 2021 Nov 24.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.

In this study, we have developed two facile spectroscopic methods for quantifying oxybutynin (OBT) hydrochloride in its pure form and tablets using design of experiments (DOEs). The spectroscopic methods depended on the ion-pair complex formation between the tertiary amino group in the drug and eosin in 0.2 M acetate buffer of pH 4. Read More

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November 2021

Chemical Modification of Glycoproteins' Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase.

Polymers (Basel) 2021 Nov 10;13(22). Epub 2021 Nov 10.

Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.

Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein's surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. Read More

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November 2021

Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems.

Life (Basel) 2021 Oct 26;11(11). Epub 2021 Oct 26.

Centre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing, which is mainly chromatography-based, of pDNA remains the key manufacturing step. Read More

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October 2021

Heterologous Expression of a Thermostable Chitinase from and Its Application for High Yield Production of Glucosamine from Shrimp Shell.

Foods 2021 Nov 15;10(11). Epub 2021 Nov 15.

School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China.

Glucosamine (GlcN) is a widely used food supplement. Hence, enormous attention has been concerned with enzymatic production of GlcN owing to its advantage over a chemical approach. In this study, a previously unstudied chitinase gene (MxChi) in the genome of was cloned, expressed in recombinant soluble form and purified to homogeneity. Read More

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November 2021

Enantioselective recognition of chiral acids by supramolecular interactions with chiral AIEgens.

Chem Commun (Camb) 2021 Nov 23. Epub 2021 Nov 23.

Shenzhen Institute of Aggregate Science and Technology, School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, China.

Novel chiral AIEgens bearing optically pure amino groups were synthesized and showed excellent discrimination for a series of chiral acidic compounds and amino acids. Interestingly, after supramolecular assembly with 4-sulfocalix[4]arene, the obtained complexes showed enhanced enantioselectivity for chiral acids. Read More

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November 2021

Formulation, Physical Stability, and the Clinical Effectiveness of Extemporaneous Ivermectin Oral Solution.

Int J Pharm Compd 2021 Nov-Dec;25(6):523-527

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine.

Ivermectin is a gamma amino butyric acid (GABA)-gated-Cl-channels modulator, which has been used orally in the treatment of numerous parasitic infections. The study target was to set up a stable, efficient 0.2% w/v ivermectin solution, which would be achieved from pure ivermectin powder as a source of the active pharmaceutical ingredient. Read More

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November 2021

ProSeqViewer: an interactive, responsive and efficient TypeScript library for visualization of sequences and alignments in web applications.

Bioinformatics 2021 Nov 12. Epub 2021 Nov 12.

Department of Biomedical Sciences, University of Padua, Padova, 35121, Italy.

Summary: Biological data is ever-increasing in amount and complexity. The mapping of this data to biological entities such as nucleotide and amino acid sequences supports biological data analysis, classification and prediction. Sequence alignments and comparison allow the transfer of knowledge to evolutionary related entities, the mapping of functional domains, the identification of binding and modification sites. Read More

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November 2021

Biosynthesis of l-phosphinothricin with enzymes from chromosomal integrated expression in .

3 Biotech 2021 Nov 28;11(11):477. Epub 2021 Oct 28.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014 China.

Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of which only the l-phosphinothricin (L-PPT) gives herbicidal function. Synthesis of optically pure L-PPT by deracemization of D/L-PPT is a promising way to cut down the environmental burden and manufacturing cost. Read More

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November 2021

Phenylalanine 314 is essential for the activity of maltogenic amylase from Corallococcus sp. EGB.

Biotechnol Appl Biochem 2021 Nov 14. Epub 2021 Nov 14.

Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, P. R. China.

Maltogenic amylase CoMA from Corallococcus sp. strain EGB catalyzes the hydrolysis and transglycosylation of maltooligosaccharides and soluble starch into maltose, the sole hydrolysate. This process yields pure maltose with potentially wide applications. Read More

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November 2021

Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil.

Saudi J Biol Sci 2021 Nov 6;28(11):6077-6085. Epub 2021 Sep 6.

Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan.

Impact of environmental perturbations i.e., nitrogen (N), phosphorus (P), potassium (K), and rice straw (Rs) on the dynamics of soil bacterial and archaeal communities are multifactor dependent and seeks a contemporary approach to study underlying mechanisms. Read More

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November 2021

Bioconversion of Apple Pomace into Microbial Protein Feed Based on Extrusion Pretreatment.

Appl Biochem Biotechnol 2021 Nov 11. Epub 2021 Nov 11.

School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255049, Shandong, China.

Apple pomace (AP) is often used directly as animal feed, while the value of feeding is limited by its low protein content. In this study, extrusion pretreatment was performed for AP, and further fermentation was carried out to improve its nutrition value. Strains suitable for extruded apple pomace (EAP) to produce high-quality microbial protein (MP) feed were screened from 12 different strains. Read More

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November 2021

Dual-Functional Coordination Polymer with High Proton Conductivity and a Low-Detection-Limit Fluorescent Probe.

J Phys Chem B 2021 Nov 8;125(45):12627-12635. Epub 2021 Nov 8.

College of Chemistry, Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), Nankai University, Tianjin 300071, P. R. China.

A coordination polymer with dual functions of high proton conductivity and highly sensitive fluorescent sensors demonstrates a great application potential. In this work, a cadmium-based coordination polymer (denoted as CP ) with hydrothermal stability was synthesized. The abundant coordination water, lattice water, and amino groups make an extended hydrogen-bonding pathway for efficient proton migration, which endows CP with the highest proton conductivity of 2. Read More

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November 2021

Constant pH molecular dynamics of porcine circovirus 2 capsid protein reveals a mechanism for capsid assembly.

Phys Chem Chem Phys 2021 Nov 10;23(43):24617-24626. Epub 2021 Nov 10.

Laboratory for Computational Molecular Design, RIKEN Center for Biosystems Dynamics Research (BDR), 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.

Spatiotemporal regulation of viral capsid assembly ensures the selection of the viral genome for encapsidation. The porcine circovirus 2 is the smallest autonomously replicating pathogenic virus, yet how PCV2 capsid assembly is regulated to occur within the nucleus remains unknown. We report that pure PCV2 capsid proteins, in the absence of nucleic acids, require acidic conditions to assemble into empty capsids . Read More

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November 2021

Effects of dairy and plant protein on growth and growth biomarkers in a piglet model.

Food Funct 2021 Nov 15;12(22):11625-11640. Epub 2021 Nov 15.

Department of Animal Science, Aarhus University, Tjele, Denmark.

The increasing world population with improved living conditions has increased the demand for food protein. This has intensified the search for sustainable alternative plant-derived high-quality protein sources for human nutrition. To study the effect of plant and milk proteins on growth in weaned pigs as a model for humans, 96 weaned pigs were divided into 48 pens and fed one of 4 different diets for 3 weeks. Read More

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November 2021

An Expanded Polyproline Domain Maintains Mutant Huntingtin Soluble and During Aging.

Front Mol Neurosci 2021 15;14:721749. Epub 2021 Oct 15.

Department of Molecular Physiology and Cell Biology, Leibniz Research Institute for Molecular Pharmacology in the Forschungsverbund Berlin e.V. (FMP), Berlin, Germany.

Huntington's disease is a dominantly inherited neurodegenerative disorder caused by the expansion of a CAG repeat, encoding for the amino acid glutamine (Q), present in the first exon of the protein huntingtin. Over the threshold of Q39 HTT exon 1 (HTTEx1) tends to misfold and aggregate into large intracellular structures, but whether these end-stage aggregates or their on-pathway intermediates are responsible for cytotoxicity is still debated. HTTEx1 can be separated into three domains: an N-terminal 17 amino acid region, the polyglutamine (polyQ) expansion and a C-terminal proline rich domain (PRD). Read More

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October 2021

Determining the enantioselectivity of asymmetric hydrogenation through parahydrogen-induced hyperpolarization.

J Chem Phys 2021 Oct;155(16):161101

Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Department of Electronic Science, Xiamen University, 361005 Xiamen, China.

Asymmetric hydrogenation plays an essential role for both academic research and industry to produce enantiomeric pure chiral molecules. Although nuclear magnetic resonance (NMR) is powerful in determining the yields of hydrogenation, it is still challenging to use NMR for chirality-related analysis. Herein, we applied parahydrogen-induced hyperpolarization (PHIP) NMR to determine the enantioselectivity of asymmetric hydrogenation and the absolute chirality of products. Read More

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October 2021

Quantitative insights into tightly and loosely bound water in hydration shells of amino acids.

Soft Matter 2021 Nov 17;17(44):10080-10089. Epub 2021 Nov 17.

CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

The hydration of amino acids closely correlates the hydration of peptides and proteins and is critical to their biological functions. However, complete and quantitative understanding about the hydration of amino acids is lacking. Here, tightly and loosely bound water of 20 zwitterionic amino acids are quantitatively distinguished and determined by Raman spectroscopy with multivariate curve resolution (Raman-MCR) and differential scanning calorimetry (DSC). Read More

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November 2021

Cyclisations and Strategies for Stereoselective Synthesis of Piperidine Iminosugars.

Chem Rec 2021 Nov 28;21(11):2958-2979. Epub 2021 Oct 28.

School of Biological and Chemical Sciences, NUI Galway, University Road, Galway, H91 TK33, Ireland.

This personal account focuses on synthesis of polyhydroxylated piperidines, a subset of compounds within the iminosugar family. Cyclisations to form the piperidine ring include reductive amination, substitution via amines, iminium ions and cyclic nitrones, transamidification (N-acyl transfer), addition to alkenes, ring contraction and expansion, photoinduced electron transfer, multicomponent Ugi reaction and ring closing metathesis. Enantiomerically pure piperidines are obtained from chiral pool precursors (e. Read More

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November 2021

Direct analysis of naphthenic acids in produced water and crude oil by NH-surface-modified wooden-tip electrospray ionization mass spectrometry.

Anal Methods 2021 Nov 18;13(44):5274-5281. Epub 2021 Nov 18.

Laboratory of Chromatography and Mass Spectrometry, Institute of Chemistry, Federal University of Goiás, Goiânia, 59078-970, GO, Brazil.

This work describes the surface coating of wooden toothpicks with amino groups (NH) for electrospray ionization mass spectrometry (MS) analysis of naphthenic acids (NAs) in produced water samples and crude oil fractions. NH was introduced into the cellulosic material through a silanization reaction using aminopropyltriethoxysilane. An NH-modified toothpick was inserted into the analyte extraction sample and was subsequently used as an electrospray emitter for MS analysis. Read More

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November 2021

Stereoselective toxicity mechanism of neonicotinoid dinotefuran in honeybees: New perspective from a spatial metabolomics study.

Sci Total Environ 2021 Oct 21:151116. Epub 2021 Oct 21.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Development of stereoisomeric neonicotinoid pesticides with lower toxicity is key to preventing global population declines of honeybees, whereas little is known about the in situ metabolic regulation of honeybees in response to stereoisomeric pesticides. Herein, we demonstrate an integrated mass spectrometry imaging (MSI) and untargeted metabolomics method to disclose disturbed metabolic expression levels and spatial differentiation in honeybees (Apis cerana) associated with stereoisomeric dinotefuran. This method affords a metabolic network mapping capability regarding a wide range of metabolites involved in multiple metabolic pathways in honeybees. Read More

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October 2021

In Situ Grafted Composite Nanoparticles-Reinforced Polyurethane Elastomer Composites with Excellent Continuous Anti-Impact Performance.

Materials (Basel) 2021 Oct 19;14(20). Epub 2021 Oct 19.

School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, China.

Polyurethane elastomer (PUE) has attracted much attention in impact energy absorption due to its impressive toughness and easy processability. However, the lack of continuous impact resistance limits its wider application. Here, an amino-siloxane (APTES) grafted WS-coated MWCNTs ([email protected]) filler was synthesized, and [email protected]/PUE was prepared by using the filler. Read More

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October 2021

Optimization of Synthesis of the Amino Lipid ECO for Effective Delivery of Nucleic Acids.

Pharmaceuticals (Basel) 2021 Oct 2;14(10). Epub 2021 Oct 2.

Department of Biomedical Engineering, School of Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.

Nucleic acids are promising for a variety of therapies, such as cancer therapy and the gene therapy of genetic disorders. The therapeutic efficacy of nucleic acids is reliant on the ability of their efficient delivery to the cytosol of the target cells. Amino lipids have been developed to aid in the cytosolic delivery of nucleic acids. Read More

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October 2021

Self-Complementary Zwitterionic Peptides Direct Nanoparticle Assembly and Enable Enzymatic Selection of Endocytic Pathways.

Adv Mater 2021 Oct 20:e2104962. Epub 2021 Oct 20.

Advanced Science Research Center at The Graduate Center of the City University of New York, 85 Saint Nicholas Terrace, New York, NY, 10031, USA.

Supramolecular self-assembly in biological systems holds promise to convert and amplify disease-specific signals to physical or mechanical signals that can direct cell fate. However, it remains challenging to design physiologically stable self-assembling systems that demonstrate tunable and predictable behavior. Here, the use of zwitterionic tetrapeptide modalities to direct nanoparticle assembly under physiological conditions is reported. Read More

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October 2021

Mass balance method for SI-traceable purity assignment of synthetic oxytocin.

J Pharm Biomed Anal 2022 Jan 29;207:114401. Epub 2021 Sep 29.

Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, PR China. Electronic address:

Oxytocin is not only a significant peptide drug for enhancing uterine contractions, but also an emerging biomarker and therapeutic target of mental disorders in clinical practice. There is a pressing need for the standardization of oxytocin assays because of its low pharmaceutical quality and large variations among measurement approaches. International System of Units (SI)-traceable analytical methods and well-characterized pure reference materials are urgently needed to set up standard reference measurement systems in laboratory medicine, ensuring the accuracy and comparability of test results. Read More

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January 2022

Synthesis and Chiroptical Properties of Planar Chiral Azahelicenes Based on [2.2]Paracyclophane.

Org Lett 2021 Nov 14;23(21):8612-8616. Epub 2021 Oct 14.

State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing 210023, Jiangsu, China.

Three pairs of planar chiral heteroarenes were synthesized using palladium-catalyzed Buchwald-Hartwig coupling and hypervalent iodine-mediated oxidative cyclization from optically pure 4-amino[2.2]paracyclophane. Among them, an enantiomer of planar chiral azahelicene was found to have circularly polarized luminescence activity that was remarkably stronger than that of planar chiral heteroarenes. Read More

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November 2021

Proteolytic Cleavage of Bioactive Peptides and Protease-Activated Receptors in Acute and Post-Colitis.

Int J Mol Sci 2021 Oct 2;22(19). Epub 2021 Oct 2.

Laboratory of Medical Biochemistry, University of Antwerp, 2610 Wilrijk, Belgium.

The protease activity in inflammatory bowel disease (IBD) and irritable bowel syndrome has been studied extensively using synthetic fluorogenic substrates targeting specific sets of proteases. We explored activities in colonic tissue from a 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis rat model by investigating the cleavage of bioactive peptides. Pure trypsin- and elastase-like proteases on the one hand and colonic tissue from rats with TNBS-induced colitis in the acute or post-inflammatory phase on the other, were incubated with relevant peptides to identify their cleavage pattern by mass spectrometry. Read More

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October 2021

Exploring the Meta-regulon of the CRP/FNR Family of Global Transcriptional Regulators in a Partial-Nitritation Anammox Microbiome.

mSystems 2021 Oct 12;6(5):e0090621. Epub 2021 Oct 12.

Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Microorganisms must respond to environmental changes to survive, often by controlling transcription initiation. Intermittent aeration during wastewater treatment presents a cyclically changing environment to which microorganisms must react. We used an intermittently aerated bioreactor performing partial nitritation and anammox (PNA) to investigate how the microbiome responds to recurring change. Read More

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October 2021

Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies.

Protein Expr Purif 2022 01 6;189:105989. Epub 2021 Oct 6.

Department of Chemistry and Biochemistry, Boise State University, Boise, ID, 83725, USA. Electronic address:

Complications related to atherosclerosis account for approximately 1 in 4 deaths in the United States and treatment has focused on lowering serum LDL-cholesterol levels with statins. However, approximately 50% of those diagnosed with atherosclerosis have blood cholesterol levels within normal parameters. Human fortilin is an anti-apoptotic protein and a factor in macrophage-mediated atherosclerosis and is hypothesized to protect inflammatory macrophages from apoptosis, leading to subsequent cardiac pathogenesis. Read More

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January 2022