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Molecular epidemiology of Bordetella pertussis and analysis of vaccine antigen genes from clinical isolates from Shenzhen, China.

Ann Clin Microbiol Antimicrob 2021 Aug 18;20(1):53. Epub 2021 Aug 18.

Shenzhen Center for Disease Control and Prevention, 8 Longyuan Road, Nanshan District, Shenzhen, China.

Background: Although pertussis cases globally have been controlled through the Expanded Programme on Immunization (EPI), the incidence of pertussis has increased significantly in recent years, with a "resurgence" of pertussis occurring in developed countries with high immunization coverage. Attracted by its fast-developing economy, the population of Shenzhen has reached 14 million and has become one of the top five largest cities by population size in China. The incidence of pertussis here was about 2. Read More

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Postoperative Low-Dose Tranexamic Acid After Major Spine Surgery: A Matched Cohort Analysis.

Neurospine 2020 Dec 31;17(4):888-895. Epub 2020 Dec 31.

Department of Anesthesiology, University of Virginia Health Science Center, Charlottesville, VA, USA.

Objective: This was a retrospective, cohort study investigating the efficacy and safety of continuous low-dose postoperative tranexamic acid (PTXA) on drain output and transfusion requirements following adult spinal deformity surgery.

Methods: One hundred forty-seven patients undergoing posterior instrumented thoracolumbar fusion of ≥ 3 vertebral levels at a single institution who received low-dose PTXA infusion (0.5-1 mg/kg/hr) for 24 hours were compared to 292 control patients who did not receive PTXA. Read More

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December 2020

Allele frequencies of Bordetella pertussis virulence-associated genes identified from pediatric patients with severe respiratory infections.

J Infect Chemother 2020 Jul 22;26(7):765-768. Epub 2020 Apr 22.

Division of Infectious Diseases, Department of Medical Specialties, National Center for Child Health and Development, Tokyo, Japan. Electronic address:

Bordetella pertussis (B. pertussis) infection occasionally causes severe respiratory infections in children. Potential association between virulence-associated gene alleles and severe clinical outcomes has been suggested; however, frequencies of these alleles in pediatric patients with severe pertussis have not been clarified. Read More

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Genome diversity and evolutionary characteristics of clinical isolates of circulating in Iran.

Iran J Microbiol 2020 Feb;12(1):1-10

Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

Background And Objectives: The re-emergence of pertussis still is being reported all over the world. Pathogen adaptation and antigenic divergence of circulating isolates from vaccine strains are the main reasons of infection resurgence. Waning immunity is also an important factor contributing to resurgence of pertussis. Read More

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February 2020

Surfaceome analysis of Australian epidemic Bordetella pertussis reveals potential vaccine antigens.

Vaccine 2020 01 6;38(3):539-548. Epub 2019 Nov 6.

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia. Electronic address:

Since acellular vaccines (ACV) were introduced in Australia, epidemic Bordetella pertussis strains changed from single nucleotide polymorphism (SNP) cluster II to SNP cluster I. Our previous proteomic analysis identified potential proteomic adaptations in the whole cell and secretome of SNP cluster I. Additionally, current ACVs were shown to be less efficacious against cluster I in mice models and there is a pressing need to discover new antigens to improve the ACV. Read More

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January 2020

Mutagenesis of N-terminal residues confer thermostability on a Penicillium janthinellum MA21601 xylanase.

BMC Biotechnol 2019 07 25;19(1):51. Epub 2019 Jul 25.

Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, No 11 Fucheng Street, Haidian District, Beijing, 100084, China.

Background: A mesophilic xylanase PjxA from Penicillium janthinellum MA21601 has high specific activity under acidic condition and holds great potential for applications in the animal feed industry. To enhance the thermostability of xylanase PjxA, two mutation strategies in the N-terminal region were examined and then integrated into the xylanase to further improvement. The recombinant xylanase PTxA-DB (The meaning of DB is disulfide-bridge. Read More

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Intrinsic planar polarity mechanisms influence the position-dependent regulation of synapse properties in inner hair cells.

Proc Natl Acad Sci U S A 2019 04 11;116(18):9084-9093. Epub 2019 Apr 11.

Institute for Auditory Neuroscience, University Medical Center Göttingen, 37075 Göttingen, Germany;

Encoding the wide range of audible sounds in the mammalian cochlea is collectively achieved by functionally diverse type I spiral ganglion neurons (SGNs) at each tonotopic position. The firing of each SGN is thought to be driven by an individual active zone (AZ) of a given inner hair cell (IHC). These AZs present distinct properties according to their position within the IHC, to some extent forming a gradient between the modiolar and the pillar IHC side. Read More

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[Antimicrobial susceptibility and antigen genotypes of Bordetella pertussis strains isolated from neonates].

Zhongguo Dang Dai Er Ke Za Zhi 2019 Mar;21(3):208-213

National Center for Children's Health/Beijing Children's Hospital, Capital Medical University/Beijing Pediatric Research Institute/National Key Discipline of Pediatrics (Capital Medical University)/National Clinical Research Center for Respiratory Diseases/Key Laboratory of Major Diseases in Children, Ministry of Education/Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing 100045, China.

Objective: To investigate the alternative antimicrobial drugs for the treatment of neonatal pertussis and the antigen genotypes of Bordetella pertussis (B. pertussis) strains.

Methods: A total of 32 B. Read More

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Bordetella pertussis Infection in Infants and Young Children in Shanghai, China, 2016-2017: Clinical Features, Genotype Variations of Antigenic Genes and Macrolides Resistance.

Pediatr Infect Dis J 2019 04;38(4):370-376

Department of Infectious Diseases, Children's Hospital of Fudan University, Shanghai, China.

Background: The global resurgence of pertussis in countries with high vaccination coverage has been a concern of public health.

Methods: Nasopharyngeal swabs were collected for Bordetella pertussis culture from children with suspected pertussis. Clinical and vaccination information were reviewed through electronic medical chart and immunization record. Read More

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Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain.

J Clin Microbiol 2019 01 2;57(1). Epub 2019 Jan 2.

Institut de Recerca Sant Joan de Déu, Hospital Sant Joan de Déu, Barcelona, Spain

This study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification of , , and , as well as its implementation in the diagnostic routine of a reference children's hospital. The new algorithm included a triplex quantitative PCR (qPCR) targeting IS gene (in , , and some strains), pIS (-specific) and P as the human internal control. Two confirmatory singleplex tests for (-Pr) and (hIS) were performed if IS was positive. Read More

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January 2019

Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico.

Epidemiol Infect 2018 12 23;146(16):2096-2101. Epub 2018 Aug 23.

Servicio de Gastroenterología,Hospital Universitario 'Dr José Eleuterio González', Universidad Autónoma de Nuevo León,Monterrey, Nuevo León,México.

We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Read More

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December 2018

A Membrane-Targeted Peptide Inhibiting PtxA of Phosphotransferase System Blocks Streptococcus mutans.

Caries Res 2019 14;53(2):176-193. Epub 2018 Aug 14.

Department of Stomatology, Nanfang Hospital, School of Stomatology, Southern Medical University, Guangzhou,

Streptococcus mutans, the primary cause of dental caries, takes up carbohydrates through the phosphoenolpyruvate sugar phosphotransferase system (PTS). This study aimed to identify a novel membrane-targeted antimicrobial peptide (AMP) that could also target the L-ascorbate-specific PtxA component of the S. mutans PTS system. Read More

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December 2019

Clinical predictor of postoperative hyperkalemia after parathyroidectomy in patients with hemodialysis.

Int J Surg 2018 May 13;53:1-4. Epub 2018 Mar 13.

Department of Otolaryngology, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou City, PR China.

Objective: Hyperkalaemia is one of the most common reasons for patients requiring emergency treatment. This study is to investigate the risk factors of postoperative hyperkalemia in renal failure patients undergoing parathyroidectomy with autotransplantation (PTXa).

Methods: One hundred and eight patients [ 54 men and 54 women; age, 45. Read More

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Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis.

PLoS One 2018 11;13(1):e0190973. Epub 2018 Jan 11.

International Center for Biotechnology, Osaka University, Suita, Osaka, Japan.

Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S. Read More

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February 2018

Resurgence of Pertussis and Emergence of the Ptxp3 Toxin Promoter Allele in South Italy.

Pediatr Infect Dis J 2018 05;37(5):e126-e131

From the Department of Biomedical Sciences and Human Oncology-Hygiene Section, University of Bari, Bari, Italy.

Background: Despite universal immunization programs, pertussis remains a major public health concern. This study aimed to describe the pertussis epidemiology in the Puglia region in 2006-2015 and to identify recent polymorphisms in Bordetella pertussis virulence-associated genes.

Methods: The pertussis cases in 2006-2015 were identified from the National Hospital Discharge Database and the Information System of Infectious Diseases. Read More

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Molecular epidemiology of Bordetella pertussis in Cambodia determined by direct genotyping of clinical specimens.

Int J Infect Dis 2017 Sep 24;62:56-58. Epub 2017 Jul 24.

Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address:

Objectives: This study sought to determine the genotypes of circulating Bordetella pertussis, the causative agent of pertussis, in Cambodia by direct molecular typing of clinical specimens.

Methods: DNA extracts from nasopharyngeal swabs obtained from 82 pertussis patients in 2008-2016 were analyzed by multilocus variable-number tandem repeat analysis (MLVA). B. Read More

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September 2017

Characterisation of the Bordetella pertussis secretome under different media.

J Proteomics 2017 03 24;158:43-51. Epub 2017 Feb 24.

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia. Electronic address:

Our understanding of the Bordetella pertussis secretome remains limited including the role of different growth conditions in the secretome. In this study the secretome of L1423, a clinical isolate from the 2008-2012 Australian epidemic, cultured on Stainer-Scholte (SS) and Thalen-IJssel (THIJS) media for 12h was characterised using liquid chromatography-mass spectrometry (LC-MS/MS). In the supernatant, LC-MS/MS identified 260 proteins with 143 bioinformatically predicted to be secreted. Read More

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Severe pertussis infection in infants less than 6 months of age: Clinical manifestations and molecular characterization.

Hum Vaccin Immunother 2017 05 27;13(5):1073-1077. Epub 2017 Jan 27.

f Virology Unit, Bambino Gesù Children Hospital IRCCS , Rome , Italy.

We conducted a study to determine the main traits of pertussis among unimmunized infants less than 6 months of age. From August 2012 to March 2015, 141 nasopharyngeal aspirates (NPAs) were collected from infants with respiratory symptoms attending 2 major hospitals in Rome. Clinical data were recorded and analyzed. Read More

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[Effect of ptxA and ptxB genes of phosphotransferase system on growth of Streptococcus mutans].

Hua Xi Kou Qiang Yi Xue Za Zhi 2016 Jun;34(3):302-6

Objective: This study aims to evaluate the effect of ptxA and ptxB genes, which are important genes in the L-ascorbate phosphotransferase system (PTS) of Streptococcus mutans (S. mutans).

Methods: The ptxA-, ptxB-, and ptxAB-double deficient mutant as well as ptxAB-complemented strain were constructed. Read More

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Identification and functional analysis of the L-ascorbate-specific enzyme II complex of the phosphotransferase system in Streptococcus mutans.

BMC Microbiol 2016 Mar 22;16:51. Epub 2016 Mar 22.

Department of Stomatology, Nanfang Hospital and College of Stomatology, Southern Medical University, Guangzhou, Guangdong, China.

Background: Streptococcus mutans is the primary etiological agent of human dental caries. It can metabolize a wide variety of carbohydrates and produce large amounts of organic acids that cause enamel demineralization. Phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) plays an important role in carbohydrates uptake of S. Read More

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[Antigenic variability of Bordetella pertussis strains isolated in 1967-2010 in the Czech Republic--possible explanation for the rise in cases of pertussis?].

Epidemiol Mikrobiol Imunol 2015 Sep;64(3):130-8

Objective: Comparison of antigenic structures of Bordetella pertussis (B. pertussis) strains isolated from 1967 to 2010 in the Czech Republic.

Material And Methods: Seventy strains of B. Read More

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September 2015

Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014).

PLoS One 2015 25;10(9):e0138941. Epub 2015 Sep 25.

Key Laboratory of Major Diseases in Children and National Key Discipline of Pediatrics (Capital Medical University), Ministry of Education, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Objectives: To investigate changes in virulence-related genotypes and in the antimicrobial susceptibility of Bordetella pertussis isolates collected from the 1970s to 2014 in the northern part of China.

Methods: A total of 124 B. pertussis isolates from three periods, the 1970s, 2000-2008, and May 2013-Sept 2014, were typed by multilocus sequence typing (MLST) and tested for antimicrobial susceptibility and virulence-related genes. Read More

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Single Amino Acid Polymorphisms of Pertussis Toxin Subunit S2 (PtxB) Affect Protein Function.

PLoS One 2015 16;10(9):e0137379. Epub 2015 Sep 16.

Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio, United States of America.

Whooping cough due to Bordetella pertussis is increasing in incidence, in part due to accumulation of mutations which increase bacterial fitness in highly vaccinated populations. Polymorphisms in the pertussis toxin, ptxA and ptxB genes, and the pertactin, prn genes of clinical isolates of Bordetella pertussis collected in Cincinnati from 1989 through 2005 were examined. While the ptxA and prn genotypes were variable, all 48 strains had the ptxB2 genotype; ptxB1 encodes glycine at amino acid 18 of the S2 subunit of pertussis toxin, while ptxB2 encodes serine. Read More

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Characterization of two Achromobacter xylosoxidans isolates from patients with pertussis-like symptoms.

Asian Pac J Trop Med 2015 Jun 25;8(6):464-7. Epub 2015 Jun 25.

ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain. Electronic address:

Objective: To characterize two Achromobacter xylosoxidans recovered from 2 patients diagnosed with pertussis during a Bordetella pertussis surveillance program.

Methods: Nasopharyngeal swabs from 2 children under 1 year of age with clinical suspicion of pertussis were analyzed by culture and PCR.

Results: Two Achromobacter xylosoxidans A8, closely related to Bordetella spp. Read More

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Genetic Variation of Bordetella pertussis in Austria.

PLoS One 2015 16;10(7):e0132623. Epub 2015 Jul 16.

Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

In Austria, vaccination coverage against Bordetella pertussis infections during infancy is estimated at around 90%. Within the last years, however, the number of pertussis cases has increased steadily, not only in children but also in adolescents and adults, indicating both insufficient herd immunity and vaccine coverage. Waning immunity in the host and/or adaptation of the bacterium to the immunised hosts could contribute to the observed re-emergence of pertussis. Read More

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Pertussis in infants less than 6 months of age and household contacts, Italy, April 2014.

Hum Vaccin Immunother 2015 ;11(5):1173-4

a Institute of Microbiology; Università Cattolica del Sacro Cuore ; Rome , Italy.

We report pertussis cases in 4 infants less than 6 months admitted with symptoms compatible with pertussis to the intensive care unit of the Università Cattolica del Sacro Cuore in Rome, April 2014. Realtime PCR confirmed pertussis diagnosis for the 4 infants, 2 of them were cousins, and for the household contacts of 1 of them. Analysis of pertussis toxin, its promoter and pertactin was also performed. Read More

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February 2016

Molecular epidemiology of Bordetella pertussis in the Philippines in 2012-2014.

Int J Infect Dis 2015 Jun 8;35:24-6. Epub 2015 Apr 8.

Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Electronic address:

Objectives: The present study was designed to determine the genotypes of circulating Bordetella pertussis in the Philippines by direct molecular typing of clinical specimens.

Methods: Nasopharyngeal swabs (NPSs) were collected from 50 children hospitalized with pertussis in three hospitals during 2012-2014. Multilocus variable-number tandem repeat analysis (MLVA) was performed on the DNA extracts from NPSs. Read More

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Survey and Rapid Detection of Bordetella pertussis in Clinical Samples Targeting the BP485 in China.

Front Public Health 2015 5;3:39. Epub 2015 Mar 5.

Institute of Disease Control and Prevention, Academy of Military Medical Sciences , Beijing , China.

Bordetella pertussis is an important human respiratory pathogen. Here, we describe a loop-mediated isothermal amplification (LAMP) method for the rapid detection of B. pertussis in clinical samples based on a visual test. Read More

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Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959-2013.

Eur J Clin Microbiol Infect Dis 2015 Jan 5;34(1):147-152. Epub 2014 Aug 5.

Department of Sera and Vaccines Evaluation, National Institute of Public Health-National Institute of Hygiene, Warsaw, Poland.

This study aimed to characterise Bordetella pertussis isolates circulating in Poland since 1959. Sequence analysis of ptxA, ptxC, prn, tcfA, fim2, fim3 and ptxP for 175 clinical isolates and currently and previously used vaccine strains was performed. Clinical isolates from the period 1995-2013 were found to be different to three currently used vaccine strains harbouring the allelic combination ptxA2-ptxC1-ptxP1-prn1-tcfA2-fim2-1-fim3-1, seen frequently in Poland in the early pertussis vaccination period but not found after 1995. Read More

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January 2015

Genetic analysis of Bordetella pertussis isolates from the 2008-2010 pertussis epidemic in Japan.

PLoS One 2013 4;8(10):e77165. Epub 2013 Oct 4.

Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan ; Department of Pediatrics, St Marianna University School of Medicine, Kawasaki, Japan.

A large pertussis epidemic occurred between 2008 and 2010 in Japan. To investigate epidemic strains, we analyzed 33 Bordetella pertussis isolates from the epidemic period by sequencing virulence-associated genes (fim3, ptxP, ptxA, and prn) and performing multilocus variable-number tandem repeat analysis (MLVA), and compared these results with those of 101 isolates from non-epidemic, earlier and later time periods. DNA sequencing of the fim3 allele revealed that the frequency of fim3B was 4. Read More

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