729 results match your criteria pte activity

Preparation of efficient, stable, and reusable copper-phosphotriesterase hybrid nanoflowers for biodegradation of organophosphorus pesticides.

Enzyme Microb Technol 2021 May 4;146:109766. Epub 2021 Mar 4.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, Jiangsu, PR China. Electronic address:

Phosphotriesterase (PTE) is considered to be a good biodegradation agent for organophosphorus pesticides. However, the instability of the free PTE limits its application. In this study, the free PTE was hybridized with copper ions (Cu) to enhance its catalytic stability and activity. Read More

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The Antithrombotic Agent Pterostilbene Interferes with Integrin αβ-Mediated Inside-Out and Outside-In Signals in Human Platelets.

Int J Mol Sci 2021 Mar 31;22(7). Epub 2021 Mar 31.

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.

Platelets play a crucial role in the physiology of primary hemostasis and pathological processes such as arterial thrombosis; thus, developing a therapeutic target that prevents platelet activation can reduce arterial thrombosis. Pterostilbene (PTE) has remarkable pharmacological activities, including anticancer and neuroprotection. Few studies have reported the effects of pterostilbene on platelet activation. Read More

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Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent Strain 52145, and Identification of Its Functional Depolymerase.

Microorganisms 2021 Mar 21;9(3). Epub 2021 Mar 21.

Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti st. 12., H-7624 Pécs, Hungary.

is among the leading bacteria that cause nosocomial infections. The capsule of this Gram-negative bacterium is a dominant virulence factor, with a prominent role in defense and biofilm formation. Bacteriophages, which are specific for one bacterial strain and its capsule type, can evoke the lysis of bacterial cells, aided by polysaccharide depolymerase enzymes. Read More

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Streptomyces pactum and sulfur mediated the rhizosphere microhabitats of potherb mustard after a phytoextraction trial.

Environ Pollut 2021 Mar 24;281:116968. Epub 2021 Mar 24.

College of Natural Resources & Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

To explore the performance of Streptomyces pactum (Act12) alone (A) and jointly with sulfur (SA) in the phytoextraction practice of potentially toxic elements (PTEs) (Cd and Zn), as well as their effects on soil chemical properties and microbial community composition, this paper selected potherb mustard (Brassica juncea, Coss.) as the test plant to assess the feedback of soil-plant ecosystems. Metal uptake values in lone Act12 treatments were higher than that of Act12 + sulfur treatments, and showed dose dependent with Act12 due to the higher biomass production. Read More

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Insights from single cell studies of human pancreatic islets and stem cell-derived islet cells to guide functional beta cell maturation in vitro.

Vitam Horm 2021 10;116:193-233. Epub 2021 Mar 10.

Stem Cells and Diabetes Laboratory, Institute of Molecular and Cell Biology (IMCB), A*STAR, Proteos, Singapore, Singapore; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. Electronic address:

There is now a sizeable number of single cell transcriptomics studies performed on human and rodent pancreatic islets that have shed light on the unique gene signatures and level of heterogeneity within each individual islet cell type. Following closely from these studies, there is also rapidly-growing activity on characterizing islet-like cells derived from in vitro differentiation of human pluripotent stem cells (hPSCs) at the single cell level. The overall consensus across the studies so far suggests that the first few stages of differentiation are largely uniform, whereas during pancreatic endocrine commitment, cell trajectories start to diverge, resulting in multiple end-stage pancreatic cells that include progenitor-like, endocrine and non-endocrine cells. Read More

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Pterostilbene promotes mitochondrial apoptosis and inhibits proliferation in glioma cells.

Sci Rep 2021 Mar 18;11(1):6381. Epub 2021 Mar 18.

Department of Neurosurgery, First Hospital of Jilin University, Changchun, 130021, Jilin Province, China.

Glioma is the most general primary and lethal intracranial malignant tumor. Pterostilbene (PTE), an analog of stilbene and resveratrol, has attracted attention in recent years due to its significant antitumor activity in multiple solid tumors; however, its effect on drug-resistant glioma cells and the underlying mechanism have not yet been reported. In this study, we found that pterostilbene inhibited proliferation, induced intrinsic mitochondria-mediated apoptosis and caused S phase arrest, inhibited migration and excessive invasion in glioma cells. Read More

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Sensitivity to change of joint count composite indices in 72 patients with systemic sclerosis.

Clin Exp Rheumatol 2021 Mar 17. Epub 2021 Mar 17.

Department of Rheumatology and Immunology, Medical School, University of Pécs, Hungary.

Objectives: We validated the responsiveness of joint count composite indices (JCCIs) in 72 patients with systemic sclerosis (SSc).

Methods: Changes in Disease Activity Score of 28 Joints using ESR and CRP (DAS28-ESR, DAS28-CRP), Simplified Disease Activity Index (SDAI) and the Clinical Disease Activity Index (CDAI) were evaluated in a one-year follow-up study. Charts of patients including swollen/tender joint counts, laboratory signs of inflammation, and visual analogue scales referring to disease activity, severity and pain were also blindly categorized by two rheumatologists as improved, unchanged or deteriorated. Read More

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Comparative Efficacy and Safety of Peficitinib Versus Tofacitinib and Baricitinib for Treatment of Rheumatoid Arthritis: A Systematic Review and Network Meta-Analysis.

Rheumatol Ther 2021 Mar 16. Epub 2021 Mar 16.

Astellas Pharma Global Development, Inc., Northbrook, USA.

Introduction: Peficitinib, a Janus kinase (JAK) inhibitor, is approved for clinical use in Japan, Korea, and Taiwan, but head-to-head comparisons versus other JAK inhibitors are lacking. We indirectly compared peficitinib, tofacitinib, and baricitinib for rheumatoid arthritis treatment.

Methods: We searched MEDLINE, Embase, the Cochrane Central Register of Controlled Trials, ClinicalTrials. Read More

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Influence of the C-terminal domain on the bioluminescence activity and color determination in green and red emitting beetle luciferases and luciferase-like enzyme.

Photochem Photobiol Sci 2021 Jan 19;20(1):113-122. Epub 2021 Jan 19.

Graduate Program of Evolutive Genetics and Molecular Biology, Federal University of São Carlos (UFSCar), São Carlos, Brazil.

Beetle luciferases catalyze the bioluminescent oxidation of D-luciferin, producing bioluminescence colors ranging from green to red, using two catalytic steps: adenylation of D-luciferin to produce D-luciferyl-adenylate and PPi, and oxidation of D-luciferyl-adenylate, yielding AMP, CO, and excited oxyluciferin, the emitter. Luciferases and CoA-ligases display a similar fold, with a large N-terminal domain, and a small C-terminal domain which undergoes rotation, closing the active site and promoting both adenylation and oxidative reactions. The effect of C-terminal domain deletion was already investigated for Photinus pyralis firefly luciferase, resulting in a red-emitting mutant with severely impacted luminescence activity. Read More

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January 2021

Neuropathophysiological Mechanisms and Treatment Strategies for Post-traumatic Epilepsy.

Front Mol Neurosci 2021 23;14:612073. Epub 2021 Feb 23.

Medical Laboratories, Department of Pediatrics, University of Iowa, Iowa City, IA, United States.

Traumatic brain injury (TBI) is a leading cause of death in young adults and a risk factor for acquired epilepsy. Severe TBI, after a period of time, causes numerous neuropsychiatric and neurodegenerative problems with varying comorbidities; and brain homeostasis may never be restored. As a consequence of disrupted equilibrium, neuropathological changes such as circuit remodeling, reorganization of neural networks, changes in structural and functional plasticity, predisposition to synchronized activity, and post-translational modification of synaptic proteins may begin to dominate the brain. Read More

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February 2021

Profiling of a Natural Alkaloid, Anatabine, in Rodents: Pharmacokinetics and Anti-Inflammatory Efficacy.

J Nat Prod 2021 Mar 11. Epub 2021 Mar 11.

Philip Morris Products S.A., Quai Jeanrenaud 5, Neuchatel, CH-2000, Switzerland.

Natural alkaloids, a large class of plant-derived substances, have attracted considerable interest because of their pharmacological activities. In this study, the pharmacokinetics and anti-inflammatory profile of anatabine, a naturally occurring alkaloid, were characterized in rodents. Anatabine was found to be bioavailable and brain-penetrant following systemic administration. Read More

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Sleep-wake characteristics in a mouse model of severe traumatic brain injury: Relation to posttraumatic epilepsy.

Epilepsia Open 2021 Mar 15;6(1):181-194. Epub 2021 Jan 15.

Department of Neurology University of Wisconsin School of Medicine and Public Health Madison WI USA.

Study Objectives: Traumatic brain injury (TBI) results in sequelae that include posttraumatic epilepsy (PTE) and sleep-wake disturbances. Here, we sought to determine whether sleep characteristics could predict development of PTE in a model of severe TBI.

Methods: Following controlled cortical impact (CCI) or sham injury (craniotomy only), CD-1 mice were implanted with epidural electroencephalography (EEG) and nuchal electromyography (EMG) electrodes. Read More

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SPR965, a Dual PI3K/mTOR Inhibitor, as a Targeted Therapy in Ovarian Cancer.

Front Oncol 2020 15;10:624498. Epub 2021 Feb 15.

Division of Gynecologic Oncology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

SPR965 is an inhibitor of PI3K and mTOR C1/C2 and has demonstrated anti-tumorigenic activity in a variety of solid tumors. We sought to determine the effects of SPR965 on cell proliferation and tumor growth in human serous ovarian cancer cell lines and a transgenic mouse model of high grade serous ovarian cancer (KpB model) and identify the underlying mechanisms by which SPR965 inhibits cell and tumor growth. SPR965 showed marked anti-proliferative activity by causing cell cycle arrest and inducing cellular stress in ovarian cancer cells. Read More

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February 2021

Validation of a diagnosis-agnostic symptom questionnaire for asthma and/or COPD.

ERJ Open Res 2021 Jan 1;7(1). Epub 2021 Feb 1.

Woolcock Institute of Medical Research, University of Sydney, Sydney, Australia.

Background: The Respiratory Symptoms Questionnaire (RSQ) is a novel, four-item patient-reported diagnosis-agnostic tool designed to assess the frequency of respiratory symptoms and their impact on activity, without specifying a particular diagnosis. Our objective was to examine its validity in patients with asthma and/or chronic obstructive pulmonary disease (COPD).

Methods: Baseline data were randomly sampled from patients who completed the RSQ in the NOVELTY study (ClinicalTrials. Read More

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January 2021

Capsaicin-Sensitive Peptidergic Sensory Nerves Are Anti-Inflammatory Gatekeepers in the Hyperacute Phase of a Mouse Rheumatoid Arthritis Model.

Int J Mol Sci 2021 Feb 8;22(4). Epub 2021 Feb 8.

János Szentágothai Research Centre, Molecular Pharmacology Research Team, University of Pécs, H-7624 Pécs, Hungary.

Capsaicin-sensitive peptidergic sensory nerves play complex, mainly protective regulatory roles in the inflammatory cascade of the joints via neuropeptide mediators, but the mechanisms of the hyperacute arthritis phase has not been investigated. Therefore, we studied the involvement of these afferents in the early, "black box" period of a rheumatoid arthritis (RA) mouse model. Capsaicin-sensitive fibres were defunctionalized by pretreatment with the ultrapotent capsaicin analog resiniferatoxin and arthritis was induced by K/BxN arthritogenic serum. Read More

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February 2021

First isolation of Candida oceani from a clinical specimen.

Antonie Van Leeuwenhoek 2021 Mar 3;114(3):337-340. Epub 2021 Feb 3.

Department of Laboratory Medicine, Microbiology Division, National University Hospital (S) Pte Ltd, 1E Kent Ridge Road, Singapore, 119228, Singapore.

We describe to our knowledge, the first documentation of Candida oceani isolate from human skin punch biopsy. Susceptibility testing was performed using Sensititre YeastOne YO10 microplate method and all common clinical antifungals appeared to have good activity against the isolate. Whole genome sequencing was also performed to provide a C. Read More

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Environmental Impact Assessment in the Former Mining Area of Regoufe (Arouca, Portugal): Contributions to Future Remediation Measures.

Int J Environ Res Public Health 2021 01 28;18(3). Epub 2021 Jan 28.

GeoBioTec-Department of Geosciences, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.

The W-Sn Regoufe mine, closed since the 1970s, was once intensively exploited for tungsten concentrates. Throughout its activity, considerable amounts of arsenopyrite-rich mine wastes were produced and, to this day, are still exposed to weathering conditions. Thus, this work aims at assessing soil contamination, using a combination of chemical, physicochemical and mineralogical analyses and sequential selective chemical extraction of the main potentially toxic elements (PTEs) in topsoils. Read More

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January 2021

Discovery of 6-Oxo-4-phenyl-hexanoic acid derivatives as RORγt inverse agonists showing favorable ADME profile.

Bioorg Med Chem Lett 2021 Mar 23;36:127786. Epub 2021 Jan 23.

Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan; Center for Research and Education on Drug Discovery, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan. Electronic address:

The retinoic acid receptor-related orphan nuclear receptor gamma t (RORγt), which is a promising therapeutic target for immune diseases, is a major transcription factor of genes related to psoriasis pathogenesis, such as interleukin (IL)-17A, IL-22, and IL-23R. Inspired by the co-crystal structure of RORγt, a 6-oxo-4-phenyl-hexanoic acid derivative 6a was designed, synthesized, and identified as a ligand of RORγt. The structure-activity relationship (SAR) studies in 6a, which focus on the improvement of its membrane permeability profile by introducing chlorine atoms, led to finding 12a, which has a potent RORγt inhibitory activity and a favorable pharmacokinetic profile. Read More

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Activity in nature mediates a park prescription intervention's effects on physical activity, park use and quality of life: a mixed-methods process evaluation.

BMC Public Health 2021 Jan 22;21(1):204. Epub 2021 Jan 22.

Saw Swee Hock School of Public Health, National University of Singapore, Block MD1, 12 Science Drive 2, #10-01, Singapore, 117549, Singapore.

Background: This process evaluation explored the implementation and mechanisms of impact of a Park Prescription Intervention trial (PPI), including the effects of hypothesised mediators (motivation, social support, recreational physical activity [PA], park use and park PA) on trial outcomes.

Methods: Participants from the community were randomly allocated to intervention (n = 80) or control (n = 80) group. The intervention included baseline counselling, a prescription of exercise in parks, materials, three-month follow-up counselling and 26 weekly group exercise sessions in parks. Read More

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January 2021

PACAP and NAP: Effect of Two Functionally Related Peptides in Diabetic Retinopathy.

J Mol Neurosci 2021 Jan 5. Epub 2021 Jan 5.

Department of Biomedical and Biotechnological Sciences, Section of Anatomy, Histology and Movement Sciences, University of Catania, Catania, Italy.

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a peptide involved in physio-pathological processes of the eye. It exerts multiple effects directly through activation of its related receptors and indirectly through increases in the synthesis of activity-dependent neuroprotective protein (ADNP). To study the role of ADNP and protect against ADNP deficiencies, a small peptide called NAP was synthetized. Read More

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January 2021

Neural competition between concurrent speech production and other speech perception.

Neuroimage 2021 03 30;228:117710. Epub 2020 Dec 30.

Cognitive and Affective Neuroscience Unit, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland; Center for the Interdisciplinary Study of Language Evolution (ISLE), University of Zurich, Switzerland; Department of Psychology, University of Oslo, Norway. Electronic address:

Understanding others' speech while individuals simultaneously produce speech utterances implies neural competition and requires specific mechanisms for a neural resolution given that previous studies proposed opposing signal dynamics for both processes in the auditory cortex (AC). We here used neuroimaging in humans to investigate this neural competition by lateralized stimulations with other speech samples and ipsilateral or contralateral lateralized feedback of actively produced self speech utterances in the form of various speech vowels. In experiment 1, we show, first, that others' speech classifications during active self speech lead to activity in the planum temporale (PTe) when both self and other speech samples were presented together to only the left or right ear. Read More

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Machine Learning-Based Automatic Classification of Video Recorded Neonatal Manipulations and Associated Physiological Parameters: A Feasibility Study.

Children (Basel) 2020 Dec 22;8(1). Epub 2020 Dec 22.

Division of Neonatology, University of California, San Francisco, CA 92521, USA.

Our objective in this study was to determine if machine learning (ML) can automatically recognize neonatal manipulations, along with associated changes in physiological parameters. A retrospective observational study was carried out in two Neonatal Intensive Care Units (NICUs) between December 2019 to April 2020. Both the video and physiological data (heart rate (HR) and oxygen saturation (SpO)) were captured during NICU hospitalization. Read More

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December 2020

[Dynamic changes of renin-angiotensin-aldosterone system parameters after surgery of primary hyperparathyroidism].

Ter Arkh 2020 Nov 24;92(10):63-69. Epub 2020 Nov 24.

Endocrinology Research Centre.

Aim: To study an activity of the Renin-Angiotensin-Aldosterone System (RAAS) components in patients with primary hyperparathyroidism (PHPT) before and after parathyroidectomy (PTE).

Materials And Methods: A comparative study of patients with PHPT and control group. The first stage of the study included 56 patients with PHPT (group 1) before and on the third day after PTE. Read More

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November 2020

Structure-activity relationship of diameter controlled Ag@Cu nanoparticles in broad-spectrum antibacterial mechanism.

Mater Sci Eng C Mater Biol Appl 2021 Feb 12;119:111501. Epub 2020 Sep 12.

Magna International Pte Ltd, Singapore.

Current outbreaks associated with drug-resistant clinical strains are demanding for the development of broad-spectrum antibacterial agents. The bactericidal materials should be eco-friendly, economical and effective to suppress bacterial growth. Thus, in this work, diameter controlled spherical Cu-Ag nanoparticles (Ag@CuNPs) with diameter ranging from 70 to 100 nm by one-step co-reduction approach were designed and synthesized. Read More

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February 2021

In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice.

Br Dent J 2020 Dec 10. Epub 2020 Dec 10.

Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Malaysia.

Introduction Virus particles in respiratory droplets and aerosols generated during medical/dental procedures are a potential source of SARS-CoV-2 cross infection. In the dental setting, oral decontamination could be an important adjunct to personal protective equipment and is recommended by a number of national COVID-19 guidance documents for dental settings.Aim To assess the in vitrovirucidal activity of an oral povidone iodine (PVP-I) product against SARS-CoV-2. Read More

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December 2020

MP-AzeFlu Improves the Quality-of-Life of Patients with Allergic Rhinitis.

J Asthma Allergy 2020 2;13:633-645. Epub 2020 Dec 2.

Rhinology Unit & Smell Clinic, ENT Department, Hospital Clinic Barcelona IDIBAPS University of Barcelona, CIBERES, Barcelona, Catalonia, Spain.

Purpose: Patients with poorly controlled allergic rhinitis (AR) experience nasal symptoms, sleep disturbances, activity impairment, and decreased quality-of-life (QoL). MP-AzeFlu is safe and effective for moderate-to-severe seasonal and perennial AR, but its impact on QoL requires investigation in the real-world, especially among phenotypes of immunoglobulin (Ig)E-mediated AR. This subanalysis of an observational study evaluated response to MP-AzeFlu via assessment of sleep quality and trouble with daily activities. Read More

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December 2020

Effects of D dopamine receptor activation in the ventral pallidum on sensory gating and food-motivated learning in control and schizophrenia model (Wisket) rats.

Behav Brain Res 2021 Feb 3;400:113047. Epub 2020 Dec 3.

Department of Physiology, Faculty of Medicine, University of Szeged, Szeged, Hungary.

Dopamine D receptors (DRs) of the ventral pallidum (VP) play important role in motivational and learning processes, however, their potential role in triggering schizophrenic symptoms has not been investigated, yet. In the present experiments the effects of locally administered DR agonist quinpirole were investigated on behavioral parameters related to sensorimotor gating, motor activity and food-motivated labyrinth learning. Two weeks after bilateral implantation of microcannulae into the VP, the acute (30 min) and delayed (3, 21 and 24 h) effects of quinpirole microinjection (1 μg/0. Read More

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February 2021

Deep Neural Network for Slip Detection on Ice Surface.

Sensors (Basel) 2020 Dec 2;20(23). Epub 2020 Dec 2.

The Kite Research Institute, Toronto Rehabilitation Institute-University Health Network, University of Toronto, Toronto, ON M5G 2A2, Canada.

Slip-induced falls are among the most common causes of major occupational injuries and economic loss in Canada. Identifying the risk factors associated with slip events is key to developing preventive solutions to reduce falls. One factor is the slip-resistance quality of footwear, which is fundamental to reducing the number of falls. Read More

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December 2020

Fine and coarse particulate matter, trace element content, and associated health risks considering respiratory deposition for Ergene Basin, Thrace.

Sci Total Environ 2021 Feb 27;754:142026. Epub 2020 Aug 27.

Dept. of Environmental Engineering, Izmir Institute of Technology, Urla, Izmir, Turkey. Electronic address:

Ergene Basin is located in Thrace, Turkey, where industries are densely populated. This study aimed to determine exposure of people living in Ergene Basin (Çorlu and Çerkezköy) to fine and coarse PM, and its potentially toxic element (PTE) content by considering variation in respiratory airway deposition rates with daily activities and PM particle size by employing deposition models of International Commission on Radiological Protection and Multiple Path Particle Dosimetry. Fine and coarse PM samples were collected daily for a year at points in Çorlu and Çerkezköy representing urban and industrial settings, respectively. Read More

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February 2021