Biomed Chromatogr 2021 Apr 30:e5153. Epub 2021 Apr 30.
State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
Peiyuan Tongnao Capsule (PTC) plays an important role in clinical application due to its excellent curative efficacy in the treatment of ischemic stroke and chronic cerebral circulation insufficiency. To standardize and rationalize the clinical application of PTC, a rapid and sensitive method based on ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry (UHPLC/Q-Orbitrap-MS) with parallel reaction monitoring mode (PRM) was developed and validated for the pharmacokinetic study. Ten bioactive compounds (aucubin, salidroside, echinacoside, paeoniflorin, verbascoside, liquiritin, 2, 3, 5, 4'-tetrahydroxy stilbene-2-O-β-D-glucoside, coumarin, glycyrrhizic acid, and emodin) were simultaneously determined in rat plasma. Read More