Mol Cell Proteomics 2002 Jan;1(1):46-59
The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences, The Neuropsychiatric Institute and the Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA.
Proteomics seeks to address the entire complement of protein gene products of an organism, but experimental analysis of such complex mixtures is biased against low abundance and membrane proteins. Electrospray-ionization mass spectrometry coupled with reverse-phase chromatography was used to separate and catalogue all detectable proteins in samples of photosystem II-enriched thylakoid membrane subdomains (grana) from pea and spinach. Around 90 intact mass tags were detected corresponding to approximately 40 gene products with variable post-translational covalent modifications. Read More