10 results match your criteria prvabc59 infect

  • Page 1 of 1

Inhibition of innate immune response ameliorates Zika virus-induced neurogenesis deficit in human neural stem cells.

PLoS Negl Trop Dis 2021 Mar 3;15(3):e0009183. Epub 2021 Mar 3.

Department of Neuroscience, Cell Biology and Anatomy, University of Texas Medical Branch, Galveston, Texas, United States of America.

Global Zika virus (ZIKV) outbreaks and their strong link to microcephaly have raised major public health concerns. ZIKV has been reported to affect the innate immune responses in neural stem/progenitor cells (NS/PCs). However, it is unclear how these immune factors affect neurogenesis. Read More

View Article and Full-Text PDF

mRNA and miRNA profiling of Zika virus-infected human umbilical cord mesenchymal stem cells identifies miR-142-5p as an antiviral factor.

Emerg Microbes Infect 2020 Dec;9(1):2061-2075

Department of Biomedical Sciences, College of Medicine, Korea University Guro Hospital, Seoul, Republic of Korea.

Zika virus (ZIKV) infection during pregnancy is associated with congenital brain abnormalities, a finding that highlights the urgent need to understand mother-to-fetus transmission mechanisms. Human umbilical cord mesenchymal stem cells (hUCMSCs) are susceptible to ZIKV infection but the underlying mechanisms of viral susceptibility remain largely unexplored. In this study, we have characterized and compared host mRNA and miRNA expression profiles in hUCMSCs after infection with two lineages of ZIKV, African (MR766) and Asian (PRVABC59). Read More

View Article and Full-Text PDF
December 2020

Susceptibility and interactions between Aedes mosquitoes and Zika viruses.

Insect Sci 2020 Jul 29. Epub 2020 Jul 29.

Department of Pathogen Biology, Key Laboratory of Tropical Disease Research of Guangdong Province, School of Public Health, Southern Medical University, Guangzhou, China.

Zika virus disease is caused by Zika virus infection, as transmitted by Aedes spp. mosquitoes. Many of the Zika virus strains isolated from patients display different pathogenicities toward humans. Read More

View Article and Full-Text PDF

Strain-Dependent Activity of Zika Virus and Exposure History in Serological Diagnostics.

Trop Med Infect Dis 2020 Mar 3;5(1). Epub 2020 Mar 3.

Department of Comparative Diagnostic and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA.

Zika virus (ZIKV) circulates as two separate lineages, with significant genetic variability between strains. Strain-dependent activity has been reported for dengue virus, herpes simplex virus and influenza. Strain-dependent activity of subject specimens to a virus could be an impediment to serological diagnosis and vaccine development. Read More

View Article and Full-Text PDF

Vector competence of mosquitoes (Diptera: Culicidae) in Zika virus transmission: an integrative review.

Rev Panam Salud Publica 2020 3;44:e7. Epub 2020 Feb 3.

Programa de Pós-Graduação em Ciências Aplicadas a Produtos Para Saúde, Faculdade de Farmácia Universidade Federal Fluminense NiteróiRio de Janeiro Brasil Programa de Pós-Graduação em Ciências Aplicadas a Produtos Para Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brasil.

Objective: To identify studies on the competence of mosquitoes as vectors for the transmission of Zika virus (ZIKV) around the globe.

Methods: We performed an integrative review to identify relevant articles on specific experiments to determine whether mosquitoes are vectors for ZIKV. The sources we used for our research were the Brazilian Periódicos CAPES electronic portal (MEDLINE/PubMed, ScienceDirect Journals, Nature Publishing Group, SciELO, Springer Link, and 250 other databases) and gray literature. Read More

View Article and Full-Text PDF
February 2020

A Novel Mechanism for Zika Virus Host-Cell Binding.

Viruses 2019 11 28;11(12). Epub 2019 Nov 28.

Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England; Armidale NSW 2351, Australia.

Zika virus (ZIKV) recently emerged in the Western Hemisphere with previously unrecognized or unreported clinical presentations. Here, we identify two putative binding mechanisms of ancestral and emergent ZIKV strains featuring the envelope (E) protein residue asparagine 154 (ASN154) and viral phosphatidylserine (PS). Synthetic peptides representing the region containing ASN154 from strains PRVABC59 (Puerto Rico 2015) and MR_766 (Uganda 1947) were exposed to neuronal cells and fibroblasts to model ZIKV E protein/cell interactions and bound MDCK or Vero cells and primary neurons significantly. Read More

View Article and Full-Text PDF
November 2019

Interferon-stimulated gene 15 (ISG15) restricts Zika virus replication in primary human corneal epithelial cells.

Ocul Surf 2019 07 22;17(3):551-559. Epub 2019 Mar 22.

Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University, Detroit, MI, USA; Department of Biochemistry, Microbiology and Immunology, Wayne State University, Detroit, MI, USA. Electronic address:

Purpose: Zika virus (ZIKV) has emerged as an important human pathogen causing ocular complications. There have been reports of the shedding of ZIKV in human as well as animal tears. In this study, we investigated the infectivity of ZIKV in corneal epithelial cells and their antiviral immune response. Read More

View Article and Full-Text PDF

Toll-like receptor 3 regulates Zika virus infection and associated host inflammatory response in primary human astrocytes.

PLoS One 2019 8;14(2):e0208543. Epub 2019 Feb 8.

Department of Immunology, Florida International University, Herbert Wertheim College of Medicine, Miami, Florida, United States of America.

The connection between Zika virus (ZIKV) and neurodevelopmental defects is widely recognized, although the mechanisms underlying the infectivity and pathology in primary human glial cells are poorly understood. Here we show that three isolated strains of ZIKV, an African strain MR766 (Uganda) and two closely related Asian strains R103451 (Honduras) and PRVABC59 (Puerto Rico) productively infect primary human astrocytes, although Asian strains showed a higher infectivity rate and increased cell death when compared to the African strain. Inhibition of AXL receptor significantly attenuated viral entry of MR766 and PRVABC59 and to a lesser extend R103451, suggesting an important role of TAM receptors in ZIKV cell entry, irrespective of lineage. Read More

View Article and Full-Text PDF
October 2019

Determination of the Cell Permissiveness Spectrum, Mode of RNA Replication, and RNA-Protein Interaction of Zika Virus.

BMC Infect Dis 2017 03 31;17(1):239. Epub 2017 Mar 31.

Department of Microbiology, Howard University College of Medicine, Seeley Mudd Building, Room 315, 520 W Street, NW, Washington, DC, 20059, USA.

Background: Two lineages of Zika virus (ZIKV) have been classified according to the phylogenetic analysis: African and Asian lineages. It is unclear whether differences exist between the two strains in host cell permissiveness, this information is important for understanding viral pathogenesis and designing anti-viral strategies.

Methods: In the present study, we comparatively studied the permissive spectrum of human cells for both the African (MR766) and Asian strains (PRVABC59) using an RNA in situ hybridization (RISH) to visualize RNA replication, an immunofluorescence technology, and a western blot assay to determine viral protein production, and a real-time RT-PCR to examine viral RNA multiplication level. Read More

View Article and Full-Text PDF

A cDNA Clone-Launched Platform for High-Yield Production of Inactivated Zika Vaccine.

EBioMedicine 2017 Mar 6;17:145-156. Epub 2017 Feb 6.

Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA; Institute for Translational Science, Galveston, TX, USA; Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, USA; Department of Pathology, Pará State University, Belém, Brazil; Department of Pharmacology & Toxicology, University of Texas Medical Branch, Galveston, TX, USA. Electronic address:

A purified inactivated vaccine (PIV) using the Zika virus (ZIKV) Puerto Rico strain PRVABC59 showed efficacy in monkeys, and is currently in a phase I clinical trial. High-yield manufacture of this PIV is essential for its development and vaccine access. Here we report an infectious cDNA clone-launched platform to maximize its yield. Read More

View Article and Full-Text PDF
  • Page 1 of 1