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Vaginal temperature before calving assessed with wireless vaginal temperature sensor in dairy and beef cattle.

Theriogenology 2021 Jul 14;172:230-238. Epub 2021 Jul 14.

Kyushu Okinawa Agricultural Research Center, NARO, Kumamoto, Japan. Electronic address:

We evaluated the daily and hourly vaginal temperature changes and the relationships between the dams' breed and parity by using a commercially available vaginal temperature sensor in 72 Holstein (Hol) calvings and 101 Japanese Black (JB) calvings. Vaginal temperature sensors inserted 7-10 days before the expected calving day sounded two alerts: when the temperature fell below the threshold (Alert 1), and when the sensor reached the ambient temperature after falling out of the dam's vagina with the rupture of the allantoic sac (Alert 2). The durations from Alert 1 to Alert 2 (Time 1) and from Alert 2 to delivery (Time 2) were calculated. Read More

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Lysophosphatidylcholine induces apoptosis and inflammatory damage in brain microvascular endothelial cells via GPR4-mediated NLRP3 inflammasome activation.

Toxicol In Vitro 2021 Jul 19:105227. Epub 2021 Jul 19.

Department of Neurology, University of Chinese Academy of Sciences Shenzhen Hospital (Guang ming), No. 39 Huaxia Road, Guangming District, Shenzhen 518107, China. Electronic address:

Lysophosphatidylcholine (LPC), as the main active component of oxidized low-density lipoproteins (ox-LDLs), has significant effects in cerebrovascular disease. However, the complex mechanism by which LPC functions in brain microvascular endothelial cells (BMECs) is not clearly understood. In this study, BMECs were transfected with G protein-coupled receptor 4 (GPR4) siRNA or an NLRP3-overexpression plasmid, and GPR4 expression was identified by RT-qPCR and western blotting; IL-1β, IL-18, and IL-33 levels were evaluated by ELISA. Read More

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Low-molecular weight chitosan enhances antibacterial effect of antibiotics and permeabilizes cytoplasmic membrane of Staphylococcus epidermidis biofilm cells.

Folia Microbiol (Praha) 2021 Jul 21. Epub 2021 Jul 21.

Department of Biotechnology, University of Chemistry and Technology in Prague, Technická 5, Prague 6 - Dejvice 166 28, Prague, Czech Republic.

This study evaluated the effect of low-molecular weight chitosan on Staphylococcus epidermidis, a common colonizer of joint implants and other prosthetic devices. We have also attempted to elucidate its mechanism of action. Chitosan was found to be effective against both the planktonic and biofilm cells (MIC 35-40 mg/L; MBIC 40-150 mg/L), in contrast to the antibiotics erythromycin and tetracycline with no antibiofilm activity (MBIC not found). Read More

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Transcatheter therapies for secondary mitral regurgitation in advanced heart failure: what are we aiming for?

Heart Fail Rev 2021 Jul 22. Epub 2021 Jul 22.

Division of Cardiology, San Raffaele Scientific Institute, Milan, Italy.

A severe secondary mitral regurgitation (SMR) can be found in a significant portion of patients affected by advanced heart failure (AHF). Conventional therapies (optimal medical therapy, devices, surgery) present restricted clinical efficacy in this stage of the left ventricle disease which is burdened by high mortality and morbidity rates. Although the treatment of choice is represented by heart transplantation (HTx), there is an unmet need related to the limited supply of donor hearts (as opposed to the growing prevalence of AHF) and the low eligibility of highly symptomatic patients. Read More

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Use of 1,470 nm laser for treatment of superficial venous insufficiency.

J Vasc Bras 2021 25;20:e20200244. Epub 2021 Jun 25.

Clínica Coris Medicina Vascular do Baía Sul Medical Center, Florianópolis, SC, Brasil.

Background: There are several ways to treat varicose veins of the lower limbs, among which use of 1470nm diode lasers stands out. This technique can be used to treat patients in outpatient settings, with early return to work, good esthetic results, and low rates of complications. However, variables such as the laser wavelength, the power administered in each area, the type of fiber, and the linear intravenous energy density (LEED) are still extensively discussed. Read More

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Staquorsin: A Novel Agr-Mediated Quorum Sensing Inhibitor Impairing Virulence Without Notable Resistance Development.

Front Microbiol 2021 5;12:700494. Epub 2021 Jul 5.

Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

The emergence of microbial resistance to the available antibiotics is a major public health concern, especially with the limited rate of developing new antibiotics. The utilization of anti-virulence agents is a non-conventional approach that can be used to combat microbial infection. In , many virulence factors are regulated by the Agr-mediated quorum sensing (QS). Read More

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Chain-prolongated ionic liquid electrolyte for low self-discharge all-solid-state supercapacitors at high temperature.

ChemSusChem 2021 Jul 20. Epub 2021 Jul 20.

Southwest Jiaotong University, Materials Science and Engneering, No. 111, North First, Section 2, 610031, Chengdu, CHINA.

High-power and high-safety enable supercapacitors to work efficiently at high temperatures. However, the high-temperature-induced excessively ion-transferring of electrolyte would severely aggregate the previously-overlooked yet highly-detrimental self-discharge behavior. Herein, we designed a poly (ethylene oxide) (PEO), bentonite clay & ionic liquid (IL) solid electrolytes (PCE:[email protected] 4 , PCB:[email protected] 4 and PCH:[email protected] 4 ) for dramatically self-discharge-lowered all-solid-state supercapacitors at high temperature of 70℃ via prolonging chain (EMIM + < BMIM + < HMIM + ). Read More

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Controlled release of the drug for osteoporosis from the surface of titanium implants coated with calcium titanate.

J Biomed Mater Res B Appl Biomater 2021 Jul 21. Epub 2021 Jul 21.

Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland.

The most popular drugs used to prevent osteoporosis that causes low mineral density and weakened microstructure of bones are bisphosphonates. Bisphosphonates can be administered in several ways, but each delivery method has drawbacks. Due to this, new methods of their delivery are being sought. Read More

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Toward conditional control of Smac mimetic activity by RNA-templated reduction of azidopeptides on PNA or 2'-OMe-RNA.

Biopolymers 2021 Jul 19:e23466. Epub 2021 Jul 19.

Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany.

Oligonucleotide templated reactions can be used to control the activity of functional molecules based on the presence of a specific trigger sequence. We report an RNA-controlled reaction system to conditionally restore the N-terminal amino group and thus binding affinity of azide-modified Smac mimetic compounds (SMCs) for their target protein X-linked Inhibitor of Apoptosis Protein (XIAP). Two templated reactions were compared: Staudinger reduction with phosphines and a photocatalytic reaction with Ru(bpy) (mcbpy). Read More

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DNA cyclic assembling control in an electrochemical strategy with [email protected] for determination of kanamycin.

Mikrochim Acta 2021 Jul 21;188(8):264. Epub 2021 Jul 21.

School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou, 510006, People's Republic of China.

A sensitive electrochemical strategy was established for kanamycin determination. A specific aptamer was modified on the electrode as the probe, followed by a cyclic hybridization chain reaction (HCR) with methylene blue, causing an increasing signal response. In the presence of kanamycin, it can initiatively convolve the aptamer and prevent further DNA assembling, resulting in a signal distinction sensitive to the target amount. Read More

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Preparation, Properties and Cell Biocompatibility of Room Temperature LCST-Hydrogels Based on Thermoresponsive PEO Stars.

Gels 2021 Jul 6;7(3). Epub 2021 Jul 6.

Department of Chemistry, University of Otago, Dunedin 9016, New Zealand.

A series of star and linear polymers based on a poly(ethylene oxide) core and poly(diethylene glycol ethyl ether acrylate) outer arms were synthesised by atom-transfer radical polymerization. The polydispersity of the polymers were low, showing good control of initiation and growth. They all showed lower critical solution (LCST) behaviour, and at 30% concentration most gelled at or below room temperature. Read More

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Delayed Lubricant Depletion of Slippery Liquid Infused Porous Surfaces Using Precision Nanostructures.

Langmuir 2021 Jul 21. Epub 2021 Jul 21.

Photonic Innovations Lab, Department of Electronic & Electrical Engineering, University College London, Torrington Place, London WC1E 7JE, U.K.

Slippery liquid infused porous surfaces (SLIPS) are an important class of repellent materials, comprising micro/nanotextures infused with a lubricating liquid. Unlike superhydrophobic surfaces, SLIPS do not rely on a stable air-liquid interface and thus can better manage low surface tension fluids, are less susceptible to damage under physical stress, and are able to self-heal. However, these collective properties are only efficient as long as the lubricant remains infused, which has proved challenging. Read More

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Construction of a hydroxide responsive -symmetric supramolecular gel for controlled release of small molecules.

Soft Matter 2021 Jul 21. Epub 2021 Jul 21.

Key Laboratory of Preparation and Application of Environmental Friendly Materials (Jilin Normal University), Ministry of Education, Changchun, 130103, China.

A C3-symmetric acylhydrazone-based low molecular weight gelator (BHTP) bearing three pyridine units was synthesized and it was found to form a stable supramolecular gel in the mixture solvent of DMSO-H2O. The morphology of the gel as observed by FE-SEM showed a dense sheet structure. Hydrogen bonding and π-π stacking between the gelators were determined as the non-covalent interactions for the gelation, which were investigated thoroughly using XRD, UV-Vis, 1H NMR and FT-IR instruments. Read More

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Restoring NK cells functionality via cytokine activation enhances cetuximab-mediated NK-cell ADCC: A promising therapeutic tool for HCC patients.

Mol Immunol 2021 Jul 17;137:221-227. Epub 2021 Jul 17.

Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Ein-Helwan, Cairo, 11795, Egypt. Electronic address:

Natural Killer (NK) cells are considered the first line of defense against viral infections and tumors. Several factors affect NK cytotoxic activity rendering it dysfunctional and thereby impeding the ability to scavenge abnormal cells as a part of immune escaping mechanisms induced by different types of cancers. NK cells play a crucial role augmenting the activity of various types of anticancer mAb since dysfunctional NK cells are the main reason for the low response to these therapies. Read More

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Human Papillomavirus (HPV) oral lesions surgical management with Nd:YAG laser versus blade and QMR scalpel: a single center experience in 251 cases.

J Biol Regul Homeost Agents 2021 Mar-Apr;35(2 Suppl. 1):351-356

Oral Medicine and Oral Surgery Laser Unit, University Center of Dentistry, Department of Medicine and Surgery, University of Parma, Italy.

HPVs are a large and varied group of viruses capable of infecting both animals and humans. They have evolved together with their respective hosts and are isolable in different and numerous species of birds, reptiles, marsupials and mammals. The present study aims to analyze and compare the epidemiological aspects of 251 HPV-associated benign lesions treated with three different surgical approaches. Read More

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Influence of M-EMS on Fluid Flow and Initial Solidification in Slab Continuous Casting.

Materials (Basel) 2021 Jul 1;14(13). Epub 2021 Jul 1.

State Key Laboratory of Advanced Special Steel & Shanghai Key Laboratory of Advanced Ferrometallurgy, Shanghai University, Shanghai 200444, China.

A mathematical model coupled with electromagnetic field has been developed to simulate the transient turbulence flow and initial solidification in a slab continuous casting mold under different electromagnetic stirring (EMS) currents and casting speeds. Through comparing the magnetic flux density, flow field with measured results, the reliability of the mathematical model is proved. The uniform index of solidified shell thickness has been introduced to judge the uniformity of the solidified shell. Read More

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A randomized trial of use of a modified reverse sural neurofasciocutaneous flap to extend the reconstruction range.

Ann Transl Med 2021 Jun;9(12):1000

Department of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: When a reverse sural neurofasciocutaneous flap is used to reconstruct a forefoot defect, usually, the transverse length of the flap is increased or the pivot point is lowered to ensure the reconstruction range. Therefore, proximal partial necrosis leading to surgery failure is sometimes caused by insufficient arterial supply if the flap is too long or the pivot point is too low and has no a reliable perforator in the pedicle. Herein, we describe a new method for extending the reconstruction range of the reverse sural neurofasciocutaneous flap that can provide a higher survival rate. Read More

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Dispersive liquid-liquid microextraction, an effective tool for the determination of synthetic cannabinoids in oral fluid by liquid chromatography-tandem mass spectrometry.

J Pharm Anal 2021 Jun 19;11(3):292-298. Epub 2020 Nov 19.

Teaching Committee of Ph.D. School in Natural Science and Engineering University of Verona, Verona, Italy.

In the present work, dispersive liquid-liquid microextraction (DLLME) was used to extract six synthetic cannabinoids (JWH-018, JWH-019, JWH-073, JWH-200, or WIN 55,225, JWH-250, and AM-694) from oral fluids. A rapid baseline separation of the analytes was achieved on a bidentate octadecyl silica hydride phase (Cogent Bidentate C; 4.6 mm × 250 mm, 4 μm) maintained at 37 °C, by eluting in isocratic conditions (water:acetonitrile (25:75, )). Read More

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Optimizing Genomic-Enabled Prediction in Small-Scale Maize Hybrid Breeding Programs: A Roadmap Review.

Front Plant Sci 2021 1;12:658267. Epub 2021 Jul 1.

Biometrics and Statistics Unit, International Maize and Wheat Improvement Center (CIMMYT), Carretera México - Veracruz, Texcoco, Mexico.

The usefulness of genomic prediction (GP) for many animal and plant breeding programs has been highlighted for many studies in the last 20 years. In maize breeding programs, mostly dedicated to delivering more highly adapted and productive hybrids, this approach has been proved successful for both large- and small-scale breeding programs worldwide. Here, we present some of the strategies developed to improve the accuracy of GP in tropical maize, focusing on its use under low budget and small-scale conditions achieved for most of the hybrid breeding programs in developing countries. Read More

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Neutrophil-Related Ratios Predict the 90-Day Outcome in Acute Ischemic Stroke Patients After Intravenous Thrombolysis.

Front Physiol 2021 2;12:670323. Epub 2021 Jul 2.

Department of Neurology, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background And Purpose: Mounting researches have illuminated that the neutrophil-related ratios were related to the prognosis of acute ischemic stroke (AIS). However, few have compared their predictive value and accuracy. To make such comparison and identify the best indicator on the 90-day outcome, we investigated biomarkers including neutrophil ratio (Nr), neutrophil count (Nc), lymphocyte (L), neutrophil-to-lymphocyte ratio (NLR), platelet (P or PLT), platelet-to-neutrophil ratio (PNR), NLR-to-platelet ratio (NLR/PLT), eosinophil (E), neutrophil-to-eosinophil ratio (NER), monocyte (M), and monocyte-to-neutrophil ratio (MNR). Read More

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Treatment of Adults and Young Adults with Acute Lymphoblastic Leukemia: Real Life Data from Two Centers in Slovakia.

Clin Lymphoma Myeloma Leuk 2021 Jun 20. Epub 2021 Jun 20.

Department of Hematology and Transfusiology of University Hospital in Bratislava, Faculty of Medicine Comenius University and Faculty of Medicine Slovak Medical University, Bratislava, Slovakia.

Introduction: The results of treatment of acute lymphoblastic leukemia (ALL) from the low population countries are missing in the literature.

Patients And Methods: We retrospectively examined biological characteristics and survival of 90 patients with ALL.

Results: At median follow-up 17 months, 52 men and 38 women were eligible for the analysis with median age 43 years (18-74). Read More

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Alterations in Sialylation Patterns are Significantly Associated with Imatinib Mesylate Resistance in Chronic Myeloid Leukemia.

Arch Med Res 2021 Jul 15. Epub 2021 Jul 15.

Cancer Biology Department, The Gujarat Cancer & Research Institute, Civil Hospital Campus, Asarwa, Ahmedabad 380 016, Gujarat, India. Electronic address:

Background And Aim: The study examined sialylation changes for their potential predictive value in assessment of imatinib mesylate (IM) resistance, alone and/or with BCR-ABL1 transcript variants among chronic myeloid leukemia (CML) cases.

Methods: A total of 98 CML cases (un-treated cases, IM non-responders and IM responders) were enrolled in the study. Total sialic acid (TSA) and total protein (TP) levels were estimated spectrophotometrically, the expression profiles of BCR-ABL1, ST3GAL1 and ST3GAL2 were evaluated using qRT-PCR assays and BCR-ABL1 transcript variants were identified through subjecting PCR products to agarose gel electrophoresis. Read More

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Development of a novel nanoemulgel formulation containing cumin essential oil as skin permeation enhancer.

Drug Deliv Transl Res 2021 Jul 17. Epub 2021 Jul 17.

Pharmaceutics Research Laboratory, School of Life Sciences, University of Sussex, Brighton, BN1 9QJ, UK.

Essential oils have been proposed as promising non-toxic transdermal permeation enhancers. Their use is limited because of their low water solubility. The use of nanotechnology-based strategies is one of the ways to overcome this limitation. Read More

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Comparison of S-1-based vs. capecitabine-based adjuvant chemotherapy for patients with gastric cancer: a systematic review and meta-analysis.

Eur J Clin Pharmacol 2021 Jul 17. Epub 2021 Jul 17.

The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Province, Hangzhou, People's Republic of China.

Background: S-1-based and capecitabine-based adjuvant treatments are proved efficacious for patients with gastric cancer, but conventional meta-analyses of the direct comparisons between two alternative adjuvant regimens to resection of GC have not been attempted.

Aim: The aim of this review was to compare the disease-free survival, overall survival and adverse events in patients receiving the S-1- and capecitabine-based adjuvant chemotherapies for treatment of gastric cancer (GC) patients undergoing resection.

Methods: A search of the academic literature was performed in PUBMED, SCOPUS, CENTRAL and EMBASE databases along with manual search in relevant journals for studies in English, to identify comparative studies comparing the effect of S-1-based chemotherapy and capecitabine-based adjuvant chemotherapy (AC), used in combination with surgical resection for treatment of gastric cancer. Read More

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From freshwater anammox bacteria (FAB) to marine anammox bacteria (MAB): A stepwise salinity acclimation process.

Sci Total Environ 2021 Jun 28;796:148753. Epub 2021 Jun 28.

Department of Environmental & IT Convergence Engineering, Chungnam National University, Daejeon, Republic of Korea. Electronic address:

An investigation into the effect of stepwise saline introduction (3-20 g·L NaCl) on the anaerobic ammonium oxidation (anammox) process in a lab-scale sequencing batch reactor was carried out for 252 days by evaluating the changes in influent and effluent nitrogen concentrations, conductivity, microbial extracellular polymeric substances' (EPS) ionic content, as well as stresses due to salinity, via microbial ATP analysis. It was observed that, effluent nitrogen concentrations remained stable at low saline levels of 3 g·L to 10 g·L. Nonetheless, midway through 10 g·L and the preliminary phase of 15 g·L salinity presented a very unstable, highly fluctuating as well as deteriorating effluent nitrogen concentrations. Read More

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The effect of a heat-stable xylanase on digesta viscosity, apparent metabolizable energy and growth performance of broiler chicks fed a wheat-based diet.

Poult Sci 2021 May 23;100(9):101275. Epub 2021 May 23.

Prestage Department of Poultry Science, NC State University, Raleigh, NC 27695-7608. Electronic address:

Feed costs represent a significant portion of the cost of poultry production. This study, in 3 experiments, was conducted to evaluate the effectiveness of a heat-stable xylanase (XYL) as a dietary supplement and its effect on digesta viscosity, nitrogen-corrected apparent metabolizable energy (AME), and live performance in broiler chicks. Experiment 1: the objective was to determine the effects of the amount and type of enzyme supplementation on digesta viscosity, AME, and bird performance using 7 diets. Read More

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Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse.

Bioresour Technol 2021 Jul 10;338:125505. Epub 2021 Jul 10.

School of Life Sciences, University of Science and Technology of China, Hefei, China.

The acquisition during biomass saccharification of elevated levels of fermentable sugars with lower cellulase concentration is central to ensuring an economically viable and industrially relevant hydrolytic process. Thus, using a new cellulase preparation (LT4) at low cellulase loading (2 mg protein/g dried substrate), this study assessed the possible boosting effect of integrating accessory enzymes and additives on high-solids hydrolysis of sugarcane bagasse via fed-batch feeding. Hydrolysis which commenced with initial 8% solids loading and subsequent substrate feeding of 4% solids at 6 h, 18 h, and 24 h respectively, proved optimal for the 20% high-solids saccharification producing 158 g/L total sugars and 83% glucose yield after 72 h with the combined optimized additives and accessory enzymes. Read More

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Engineering a probiotic strain of Escherichia coli to induce the regression of colorectal cancer through production of 5-aminolevulinic acid.

Microb Biotechnol 2021 Jul 16. Epub 2021 Jul 16.

State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao, Shandong, 266237, China.

Bacterial vectors can be engineered to generate microscopic living therapeutics to produce and deliver anticancer agents. Escherichia coli Nissle 1917 (Nissle 1917) is a promising candidate with probiotic properties. Here, we used Nissle 1917 to develop a metabolic strategy to produce 5-aminolevulinic acid (5-ALA) from glucose as 5-ALA plays an important role in the photodynamic therapy of cancers. Read More

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The anion of choline-based ionic liquids tailored interactions between ionic liquids and bovine serum albumin, MCF-7 cells, and bacteria.

Colloids Surf B Biointerfaces 2021 Jul 9;206:111971. Epub 2021 Jul 9.

Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China. Electronic address:

Choline-based ionic liquids (ILs) have been widely applied because of their good biocompatibility. Herein, the toxicity of choline-based ILs containing different anions was studied by UV-vis absorption spectra, fluorescence spectra, MTT assays and antibacterial experiments. The results explained that choline chloride ([Ch][Cl]) had no obvious effect on the conformation of bovine serum albumin (BSA), while the conformation could be slightly changed by choline bromide ([Ch][Br]). Read More

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Feasibility of Noninvasive Positive Pressure Ventilation in the Treatment of Oxygen-Dependent COVID-19 Patients in Peru.

Am J Trop Med Hyg 2021 Jul 16. Epub 2021 Jul 16.

School of Public Health, Cayetano Heredia University, Lima, Peru.

Intensive care is expensive, and availability is limited. Low- and middle-income countries in particular have struggled to cope with the large influx of critically ill patients during the COVID-19 pandemic. Noninvasive respiratory support devices delivering continuous positive airways pressure (CPAP) require less resource and staff expertise compared with invasive mechanical ventilators and can be routinely used outside of intensive care units. Read More

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