15 results match your criteria prototrophy recipient

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HisB as novel selection marker for gene targeting approaches in Aspergillus niger.

BMC Microbiol 2017 03 8;17(1):57. Epub 2017 Mar 8.

Institute of Biotechnology, Department of Applied and Molecular Microbiology, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355, Berlin, Germany.

Background: For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene integration and deletion in A. Read More

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Complementation of a stable Met2-1 mutant of the zygomycete Absidia glauca by the corresponding wild-type allele of the mycoparasite Parasitella parasitica, transferred during infection.

Microbiology (Reading) 2013 Aug 23;159(Pt 8):1639-1648. Epub 2013 May 23.

General Microbiology and Microbe Genetics, Friedrich-Schiller University Jena, Neugasse 24, D-07743 Jena, Germany.

Compared with prokaryotes, where horizontal gene transfer events are frequently found and can be studied in the laboratory at the mechanistic level, few systems are known that allow direct experimental access to parasexual phenomena in eukaryotes. In zygomycetes, a basal lineage of fungi, several mycoparasitic fungi are known that inevitably form a cytoplasmic continuum with their hosts during infection. We provide evidence that, corresponding to the expectation suggested by the morphology of the infection process, gene transfer occurs from the parasite to the host. Read More

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Establishment of a gene transfer system for Rhodothermus marinus.

Appl Microbiol Biotechnol 2005 Mar;66(6):675-82

Laboratory of Molecular Genetics, Institute of Biology, University of Iceland, Reykjavik, Iceland.

Genetic manipulation of Rhodothermus marinus has been hampered by the lack of a selection system for gene transfer. We report construction of a Rhodothermus/Escherichia coli shuttle plasmid, containing the R. marinus trpB gene, based on pUC18 and the cryptic R. Read More

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Natural transformation in river epilithon.

Appl Environ Microbiol 1996 Aug;62(8):2994-8

School of Pure and Applied Biology, College of Cardiff, University of Wales, United Kingdom.

Natural transformation was demonstrated in unenclosed experiments incubated in river epilithon. Strains of Acinetobacter calcoaceticus were transformed to prototrophy by either free DNA (lysates) or live donor cells. The sources of transforming DNA and recipient culture were immobilized on filters, secured to stones, and incubated midstream in the river. Read More

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Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death.

J Bacteriol 1994 Dec;176(23):7352-61

Department of Microbiology, National Research Centre for Biotechnology (GBF), Braunschweig, Germany.

Two novel conditional broad-host-range cell lysis systems have been developed for the study of natural transformation in bacteria and the environmental fate of DNA released by cell death. Plasmid pDKL02 consists of lysis genes S, R, and Rz from bacteriophage lambda under the control of the Ptac promoter. The addition of inducer to Escherichia coli, Acinetobacter calcoaceticus, or Pseudomonas stutzeri containing plasmid pDKL02 resulted in cell lysis coincident with the release of high amounts of nucleic acids into the surrounding medium. Read More

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December 1994

Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii.

Proc Natl Acad Sci U S A 1993 Oct;90(19):9199-203

Plant Science Center, Cornell University, Ithaca, NY 14853.

Nuclear transformation of the unicellular green alga Chlamydomonas reinhardtii has thus far been characterized by integration of the introduced DNA into nonhomologous sites. In this study, the occurrence of homologous recombination events during transformation was investigated with the intent of developing strategies for gene targeting and gene disruption. Homologous recombination was monitored by using nonfunctional 5' and 3' deletion derivatives of the wild-type C. Read More

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October 1993

New shuttle vectors for direct cloning in Saccharomyces cerevisiae.

Authors:
P Silar D J Thiele

Gene 1991 Jul;104(1):99-102

Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109.

We have constructed new shuttle vectors to facilitate the screening of recombinant plasmids after direct transformation of yeast cells. The vectors are pBluescript-based shuttle vectors in which the lacZ marker has been replaced by an analogous system based on the Saccharomyces cerevisiae URA3 gene. DNA fragments are inserted in a polylinker located after the beginning of the URA3 coding sequence. Read More

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Transduction of certain genes by an autonomously replicating Bacillus thuringiensis phage.

Appl Environ Microbiol 1991 Apr;57(4):1000-5

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.

A derivative of Bacillus thuringiensis subsp. kurstaki HD1 (HD1-9) released transducing phage (TP21) from late exponential cultures. Three of seven markers tested were transduced into Bacillus cereus, but only two of these (cysC and trpB/F) were transduced at a frequency of more than 100 times the reversion rates. Read More

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Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis. I. Construction and analysis of B. subtilis clone banks in Escherichia coli.

Mol Gen Genet 1984 ;193(2):299-305

Cloning in Escherichia coli and Bacillus subtilis was carried out using the bifunctional plasmid pDH5060. B. subtilis chromosomal DNA and pDH5060 DNA were digested with either BamHI or SalI, then annealed, ligated, and transformed into E. Read More

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Chromosome-mediated gene transfer with the Chinese hamster ovary cell line.

Authors:
W H Lewis

Exp Cell Res 1983 Feb;143(2):309-18

Using an improved method of chromosome-mediated gene transfer, we have investigated transfer of the codominantly expressed methotrexate-resistant dihydrofolate reductase (MtxRIIIdhfr) gene into Chinese hamster ovary (CHO) cell recipients. The frequency of dhfr gene transfer with CHO cells varied considerably from clone to clone, ranging from 4 X 10(-7) to 5 X 10(-5). Using appropriate cell recipients we were able to test for linkage of several genetic markers available in the CHO cell line. Read More

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February 1983

Genetic transformation of obligately chemolithotrophic thiobacilli.

J Bacteriol 1983 Feb;153(2):652-7

Genetic transformation of Thiobacaillus thioparus auxotrophs to prototrophy was obtained at frequencies of up to 10(-2) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent strain of the same bacterium. The rate at which transformation occurred depended on recipient growth rate and could be drastically reduced by depriving otherwise competent cells of either nitrogen or exogenous energy substrate. Interspecies marker transfer was also shown among several obligately chemolithotrophic members of the genus. Read More

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February 1983

Transforming activity of mercury-substituted DNA synthesized in vitro by permeable cells of Bacillus subtilis.

J Biol Chem 1982 Feb;257(4):1610-2

Mercurated DNA was synthesized in permeable cells of Bacillus subtilis, using 5-mercurideoxycytidine triphosphate as one of the substrates, and was separated from parental unsubstituted DNA by isopycnic centrifugation in CsCl gradients. The ability of mercurated DNA to transform auxotrophic strains of B. subtilis to prototrophy was compared with that of normal DNA and was 10-20% of the latter. Read More

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February 1982

Physiological factors involved in the transformation of Mycobacterium smegmatis.

J Bacteriol 1978 Mar;133(3):1254-62

Transfer of streptomycin resistance and changes from methionine and leucine auxotrophy to prototrophy were achieved in Mycobacterium smegmatis by transformation. Recipient cells were more resistant to mitomycin C and methyl methlanesulfonate treatments than were wild-type cells. A high level of calcium ions was essential for transformation, especially during DNA adsorption, whereas the presence of magnesium ions and the exposure of recipient cells to mild doses of UV light enhanced recombination frequencies. Read More

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Intrageneric transformation of neisseria gonorrhoeae and neisseria perflava to streptomycin resistance and nutritional independence.

J Bacteriol 1975 Dec;124(3):1359-65

Auxotrophic mutants of Neisseria gonorrhoeae and Neisseria perflava were transformed to prototrophy using homologous and heterologous deoxyribonucleic acid (DNA). Within either species the efficiencies of transformation for nutritional markers were found to be very similar to the values obtained for transformation to streptomycin resistance. The number of transformants in the interspecific N. Read More

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December 1975

Chromosome transfer in Proteus mirabilis mediated by hybrid plasmid.

Authors:
J N Coetzee

J Gen Microbiol 1975 Jan;86(1):133-46

A previously-described fused plasmid, P-lacRIdrd19, was found to mediate chromosomal transfer between cells of Proteus mirabilis strain PM5006; PM5006-(P-lacRIdrd19) was usually the donor and various auxotrophs of PM5006 resistant to nalidixic acid and/or streptomycin were recipients. The donor was usually counterselected with nalidixic acid and/or high concentrations of streptomycin. Recombination experiments with single markers indicated a 40-fold variation in recombination frequencies for different markers. Read More

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January 1975
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