281,757 results match your criteria protocols human


Experimental study protocol of the project "MOtor function and VItamin D: Toolkit for motor performance and risk Assessment (MOVIDA)".

PLoS One 2021 22;16(7):e0254878. Epub 2021 Jul 22.

Department of Cardiovascular and Endocrine-Metabolic Diseases and Aging, Italian National Institute of Health, Rome, Italy.

Musculoskeletal injuries, a public health priority also in the military context, are ascribed to several risk factors, including: increased reaction forces; low/reduced muscle strength, endurance, body mass, Vitamin D level, and bone density; inadequate lifestyles and environment. The MOVIDA Project-funded by the Italian Ministry of Defence-aims at developing a transportable toolkit (assessment instrumentation, assessment protocols and reference/risk thresholds) which integrates motor function assessment with biological, environmental and behavioural factors to help characterizing the risk of stress fracture, stress injury or muscle fatigue due to mechanical overload. The MOVIDA study has been designed following the STROBE guidelines for observational cross-sectional studies addressing healthy adults, both militaries and civilians, with varying levels of physical fitness (sedentary people, recreational athletes, and competitive athletes). Read More

View Article and Full-Text PDF

Ligands of the Neuropeptide Y Y2 Receptors as a Potential Multitarget Therapeutic Approach for the Protection of the Neurovascular Unit Against Acute Ischemia/Reperfusion: View from the Perspective of the Laboratory Bench.

Transl Stroke Res 2021 Jul 22. Epub 2021 Jul 22.

Laboratory of Experimental and Clinical Neurosurgery, Mossakowski Medical Research Institute Polish Academy of Sciences, A. Pawińskiego Str. 5, 02-106, Warsaw, Poland.

Ischemic stroke is the third leading cause of death and disability worldwide, with no available satisfactory prevention or treatment approach. The current treatment is limited to the use of "reperfusion methods," i.e. Read More

View Article and Full-Text PDF

Generation and Maintenance of Homogeneous Human Midbrain Organoids.

Bio Protoc 2021 Jun 5;11(11):e4049. Epub 2021 Jun 5.

Department for Cell and Developmental Biology, Max Planck Institute for molecular Biomedicine, Münster, Germany.

Three-dimensional cell cultures ("organoids") promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require extensive manual handling and result in heterogeneous aggregates with high sample-to-sample variation, which can hinder screening-based strategies. We have now developed a fast and efficient protocol for the generation and maintenance of highly homogeneous and reproducible midbrain organoids. Read More

View Article and Full-Text PDF

Therapeutic Hypothermia With Progesterone Improves Neurologic Outcomes in Ventricular Fibrillation Cardiac Arrest After Electric Shock.

Cureus 2021 Jun 18;13(6):e15749. Epub 2021 Jun 18.

Surgery, St. Mary's Medical Center, West Palm Beach, USA.

Trauma by electricity imposes mechanical, electrical, and thermal forces on the human body. Often, the delicate cardiac electrophysiology is disrupted causing dysrhythmia and subsequent cardiac arrest. Anoxic brain injury (ABI) is the most severe consequence and the main cause of mortality following cardiac arrest. Read More

View Article and Full-Text PDF

Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis.

Front Microbiol 2021 5;12:618566. Epub 2021 Jul 5.

Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain.

The use of metaproteomics for studying the human gut microbiota can shed light on the taxonomic profile and the functional role of the microbial community. Nevertheless, methods for extracting proteins from stool samples continue to evolve, in the pursuit of optimal protocols for moistening and dispersing the stool sample and for disrupting microbial cells, which are two critical steps for ensuring good protein recovery. Here, we evaluated different stool sample processing (SSP) and microbial cell disruption methods (CDMs). Read More

View Article and Full-Text PDF

Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells.

Front Cell Neurosci 2021 5;15:682272. Epub 2021 Jul 5.

Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA, United States.

Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variability and reproducibility as well as lack of or underrepresentation of certain cell types typically found in the brain. As the technology to generate COs has continued to improve, more efficient and streamlined protocols have addressed some of these issues. Read More

View Article and Full-Text PDF

Guided endodontic microsurgery in apicoectomy: a review.

J Biol Regul Homeost Agents 2021 May-Jun;35(3 Suppl. 1):47-55

University of Rome "Tor Vergata", Department of Chemical Science and Technologies, Rome, Italy.

The purpose of this review was twofold. The first aim was to show predictable protocols of guided endodontic microsurgery (EMS) and to describe its clinical outcomes. The second aim was to verify the accuracy of this technique compared to the traditional pathway. Read More

View Article and Full-Text PDF

Current Infection Prevention and Control Strategies of COVID-19 in Hospitals.

Turk J Med Sci 2021 Jul 22. Epub 2021 Jul 22.

Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has been appeared first in China since December 2019. Transmission of SARS-CoV-2 occurs primarily with droplets through coughing and sneezing, is also occurs through inhalation of aerosolized secretions, which travel, remain suspended in the air longer. Since early in the outbreak, have described COVID-19 cases in healthcare workers (HCWs). Read More

View Article and Full-Text PDF

Chronic limbic epilepsy models for therapy discovery: Protocols to improve efficiency.

Epilepsia 2021 Jul 21. Epub 2021 Jul 21.

SEIN: Epilepsy Institute in the Netherlands Foundation Heemstede, Heemstede, The Netherlands.

Objective: There have been recommendations to improve therapy discovery for epilepsy by incorporating chronic epilepsy models into the preclinical process, but unpredictable seizures and difficulties in maintaining drug levels over prolonged periods have been obstacles to using these animals. We report new protocols in which drugs are administered through a new chronic gastric tube to rats with higher seizure frequencies to minimize these obstacles.

Methods: Adult rats with spontaneous limbic seizures following an episode of limbic status epilepticus induced by electrical hippocampal stimulation were monitored with long-term video- electroencephalography (EEG). Read More

View Article and Full-Text PDF

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19.

ACS Biomater Sci Eng 2021 Jul 21. Epub 2021 Jul 21.

Department of Anatomy, CCB, State University of Londrina (UEL), Campus Universitário s/n, Caixa Postal 10011, 86057-970, Londrina, Parana, Brazil.

The coronavirus disease 2019 (COVID-19), caused by the novel coronavirus, SARS-CoV-2, affects tissues from different body systems but mostly the respiratory system, and the damage evoked in the lungs may occasionally result in severe respiratory complications and eventually lead to death. Studies of human respiratory infections have been limited by the scarcity of functional models that mimic in vivo physiology and pathophysiology. In the last decades, organoid models have emerged as potential research tools due to the possibility of reproducing in vivo tissue in culture. Read More

View Article and Full-Text PDF

Sensitive and Specific Immunohistochemistry Protocol for Nucleocapsid Protein from All Common SARS-CoV-2 Virus Strains in Formalin-Fixed, Paraffin Embedded Tissues.

Methods Protoc 2021 Jul 10;4(3). Epub 2021 Jul 10.

Department of Pathology, Medical College of Wisconsin, TBRC-C4980, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA.

Human coronavirus disease 2019 (COVID-19) is a life-threatening and highly contagious disease caused by coronavirus SARS-CoV-2. Sensitive and specific detection of SARS-CoV-2 viral proteins in tissues and cells of COVID-19 patients will support investigations of the biologic behavior and tissue and cell tropism of this virus. We identified commercially available affinity-purified polyclonal antibodies raised against nucleocapsid and spike proteins of SARS-CoV-2 that provide sensitive and specific detection of the virus by immunohistochemistry in formalin-fixed, paraffin-embedded tissue. Read More

View Article and Full-Text PDF

Coronavirus Disease 2019 (COVID-19) Diagnostic Tools: A Focus on Detection Technologies and Limitations.

Curr Issues Mol Biol 2021 Jul 20;43(2):728-748. Epub 2021 Jul 20.

Division of Molecular Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 4110 Libra Drive, Orlando, FL 32816, USA.

The ongoing coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a severe threat to human health and the global economy and has resulted in overwhelming stress on health care systems worldwide. Despite the global health catastrophe, especially in the number of infections and fatalities, the COVID-19 pandemic has also revolutionized research and discovery with remarkable success in diagnostics, treatments, and vaccine development. The use of many diagnostic methods has helped establish public health guidelines to mitigate the spread of COVID-19. Read More

View Article and Full-Text PDF

Prediction of ovarian response in IVF/ICSI cycles.

JBRA Assist Reprod 2021 Jul 21;25(3):422-427. Epub 2021 Jul 21.

Center for Reproductive Medicine, Universitair Ziekenhuis Brussel, Brussels, Belgium.

Objective: Our study aims to evaluate the various correlations between demographic, biochemical, ultrasound, and ovarian stimulation parameters with the percentage of mature oocytes in conventional stimulation for IVF/ICSI cycles in order to develop a predictive model to improve our understanding of the oocyte maturation process.

Methods: This is a retrospective cohort study; patients underwent conventional antagonist ovarian stimulation protocols for fresh IVF/ICSI cycles. A total of 256 IVF/ICSI cycles were included. Read More

View Article and Full-Text PDF

Preparation and Mounting of Whole Blood Clot Samples for Mechanical Testing.

Curr Protoc 2021 Jul;1(7):e197

Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas.

Studying and quantifying the mechanics of blood clots is essential to better diagnosis and prognosis of, as well as therapy for, thromboembolic pathologies such as strokes, heart attacks, and pulmonary embolisms. Unfortunately, mechanically testing blood clots is complicated by their softness and fragility, thus making the use of classic mounting techniques, such as clamping, challenging. This is particularly true for mechanical testing under large deformation. Read More

View Article and Full-Text PDF

Patterns of Maxillofacial Injuries in the Middle East and North Africa: A Systematic Review.

Int Dent J 2021 Aug 28;71(4):292-299. Epub 2021 Jan 28.

College of Dentistry, Ajman University, Ajman, United Arab Emirates.

Objective: The objective of this review was to investigate the epidemiological characteristics of maxillofacial fractures (MFFs), to establish the prevalence of MFFs, and to recognise the major causative factors in both males and females in the Middle East and North Africa (MENA) region.

Study Design: The protocol of this systematic reviews was established according to the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P); the following databases were searched: PubMed/Medline, Scopus, Google Scholar and Web of Science. We used STROBE checklist to assess the risk of bias in all identified studies, 37 studies fulfilled the eligibility criteria, and hence were selected for analysis. Read More

View Article and Full-Text PDF

Assessing Prepulse Inhibition of Startle in Mice.

Bio Protoc 2018 Apr 5;8(7):e2789. Epub 2018 Apr 5.

INSERM, U1215 NeuroCentre Magendie, Bordeaux, France.

Animal models are an important tool for studying neuropsychiatric disorders. However, a major challenge for researchers working with laboratory rodents is trying to reproduce 'core' symptoms of complex human disorders such as schizophrenia. Despite this challenge, however, it is still conceivable to use animal models designed to reproduce some of the disease's 'endo-phenotypes'. Read More

View Article and Full-Text PDF

Protocols to Study Declarative Memory Formation in Mice and Humans:Optogenetics and Translational Behavioral Approaches.

Bio Protoc 2018 Jun 20;8(12):e2888. Epub 2018 Jun 20.

Neurocentre Magendie, Physiopathologie de la Plasticité Neuronale, U1215, INSERM, Bordeaux, France.

Declarative memory formation depends on the hippocampus and declines in aging. Two functions of the hippocampus, temporal binding and relational organization (Rawlins and Tsaltas, 1983; Eichenbaum , 1992 ; Cohen , 1997 ), are known to decline in aging (Leal and Yassa, 2015). However, in the literature distinct procedures have been used to study these two functions. Read More

View Article and Full-Text PDF

PTEN Loss and ARID1A Mutation in an HPV-positive Metastatic Adenocarcinoma Diagnosed Almost 18 yr After an Intact Cone Excision for Endocervical Adenocarcinoma In Situ.

Int J Gynecol Pathol 2021 Jul 19. Epub 2021 Jul 19.

Departments of Pathology (O.A., S.Y.Z., F.H., A.A.R., O.F.) Obstetrics and Gynecology, University of California, San Diego, San Diego, California (P.B.).

There have been previous reports of neoplasms with the morphology of endocervical adenocarcinoma in situ (AIS) that secondarily involve the ovaries, presumably through transtubal spread, with a smaller subset metastasizing to distant sites. These ovarian metastases have been discovered up to 7 yr postexcision of the endocervical lesion, consistent with the known potential for overtly invasive cervical carcinomas to recur late after primary curative management. Herein, we present a case of a premenopausal woman with a pelvic mass classified as metastatic human papillomavirus (HPV)-associated endocervical adenocarcinoma (p16-block immunoreactive, high-risk HPV positive by in situ hybridization with PTEN loss, ARID1A, and PBRM1 mutations detected by qualitative next-generation sequencing), identified 17. Read More

View Article and Full-Text PDF

Effects of lower extremity constraint-induced movement therapy on gait and balance of chronic hemiparetic patients after stroke: description of a study protocol for a randomized controlled clinical trial.

Trials 2021 Jul 19;22(1):463. Epub 2021 Jul 19.

Neurology/Neuroscience program, Federal University of São Paulo - UNIFESP, Botucatu street, 862 - 5° floor Edifico Ciências Biomédicas, São Paulo, Brazil.

Background: Protocols involving intensive practice have shown positive outcomes. Constraint induced movement therapy (CIT) appears to be one of the best options for better outcomes in upper limb rehabilitation, but we still have little data about lower extremity constraint-induced movement therapy (LE-CIT) and its effects on gait and balance.

Objective: To evaluate the effects of an LE-CIT protocol on gait functionality and balance in chronic hemiparetic patients following a stroke. Read More

View Article and Full-Text PDF

Serum IgG level is a predicting factor for the response to neoadjuvant chemotherapy in patients with esophageal squamous cell carcinoma.

World J Surg Oncol 2021 Jul 19;19(1):217. Epub 2021 Jul 19.

Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya City, Aichi, 467-8602, Japan.

Background: Despite the established oncological benefits of neoadjuvant chemotherapy for esophageal squamous cell cancer, not all cases demonstrate benefit. Hence, predicting the response to chemotherapy before treatment is desirable. Some reports have shown that immune factors are related to the chemotherapy response. Read More

View Article and Full-Text PDF

Hospitalization and colonization by methicillin-resistant Staphylococcus aureus in the surgical department of 03 health facilities in the Ndé division, West-Cameroon.

Ann Clin Microbiol Antimicrob 2021 Jul 19;20(1):48. Epub 2021 Jul 19.

School of Pharmacy, Higher Institute of Health Sciences, Université Des Montagnes, Bangangté, Cameroon.

Background: Commensal flora colonization during hospitalization by bacteria is the first step for nosocomial infections while antibiotic resistance reduces therapeutic options. In aim to control this phenomenon, we initiated this study to describe the impact of hospitalization on colonization by methicillin-resistant Staphylococcus aureus in the surgical department of 03 health facilities in the Ndé division, West-Cameroon.

Methods: This study was carried out on patients admitted for surgery in 03 health facilities of the Ndé division, West-Cameroon (District Hospital of Bangangté, Protestant Hospital of Bangwa and Cliniques Universitaires des Montagnes). Read More

View Article and Full-Text PDF

Enhancing the estimation of fiber orientation distributions using convolutional neural networks.

Comput Biol Med 2021 Jul 14;135:104643. Epub 2021 Jul 14.

School of Biomedical Engineering and Imaging Sciences, King's College London, UK.

Local fiber orientation distributions (FODs) can be computed from diffusion magnetic resonance imaging (dMRI). The accuracy and ability of FODs to resolve complex fiber configurations benefits from acquisition protocols that sample a high number of gradient directions, a high maximum b-value, and multiple b-values. However, acquisition time and scanners that follow these standards are limited in clinical settings, often resulting in dMRI acquired at a single shell (single b-value). Read More

View Article and Full-Text PDF

Trajectories of antenatal depression and adverse pregnancy outcomes.

Am J Obstet Gynecol 2021 Jul 16. Epub 2021 Jul 16.

Northwestern University Feinberg School of Medicine, Department of Obstetrics and Gynecology, Division of Maternal Fetal Medicine, Chicago IL.

Background: Antenatal depression affects approximately one in seven pregnancies, with an increasing prevalence across gestation. Data regarding the associations between antenatal depression and adverse pregnancy outcomes yield conflicting results. However, previous studies evaluated the cross-sectional prevalence of depression at various time points, and not the trajectory of depressive symptoms across gestation. Read More

View Article and Full-Text PDF

The relationship between job components, neighbourhood walkability and African academics' physical activity: a post-COVID-19 context.

Health Promot Int 2021 Jul 19. Epub 2021 Jul 19.

School of Business, Accra Technical University, Accra, Ghana.

Research to date suggests that physical activity (PA) among academics is insufficient globally. Academics in many African countries were recently required to resume work while observing social distancing protocols. Physical inactivity (PI) was, therefore, expected to increase in such academics. Read More

View Article and Full-Text PDF

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice.

J Vis Exp 2021 Jun 30(172). Epub 2021 Jun 30.

Department of Biological Sciences, Ohio University; Molecular and Cellular Biology Program, Ohio University; Edison Biotechnology Institute, Ohio University; Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University;

Adenosine triphosphate (ATP), including extracellular ATP (eATP), has been shown to play significant roles in various aspects of tumorigenesis, such as drug resistance, epithelial-mesenchymal transition (EMT), and metastasis. Intratumoral eATP is 10 to 10 times higher in concentration than in normal tissues. While eATP functions as a messenger to activate purinergic signaling for EMT induction, it is also internalized by cancer cells through upregulated macropinocytosis, a specific type of endocytosis, to perform a wide variety of biological functions. Read More

View Article and Full-Text PDF

Efficacy of soap and water based skin decontamination using animal models: a systematic review.

J Toxicol Environ Health B Crit Rev 2021 Jul 18:1-12. Epub 2021 Jul 18.

Department of Dermatology, University of California San Francisco, San Francisco, California, United States.

Water-only or soap and water solutions are considered a gold standard for skin decontamination. However, there is lack of conclusive data regarding their efficacy. The aim of this study was to summarize animal model data on skin decontamination using water-only, and/or soap and water. Read More

View Article and Full-Text PDF

A Comparison Between Body Weight-Supported Treadmill Training and Conventional Over-Ground Training in Dogs With Incomplete Spinal Cord Injury.

Front Vet Sci 2021 1;8:597949. Epub 2021 Jul 1.

CIISA-Centro Interdisciplinar de Investigação em Saúde Animal-Faculty of Veterinary Medicine, Lisboa, Portugal.

In human medicine there was no evidence registered of a significant difference in recovery between body weight-supported treadmill training (BWSTT) and conventional over-ground (COGI). There isn't any similar study in veterinary medicine. Thus, this study aimed to compare the locomotor recovery obtained in incomplete SCI (T11-L3 Hansen type I) post-surgical dogs following BWSTT or COGI protocols, describing their evolution during 7 weeks in regard to OFS classifications. Read More

View Article and Full-Text PDF

Past, Present, and Future of Gonadotropin Use in Controlled Ovarian Stimulation During Assisted Reproductive Techniques.

Cureus 2021 Jun 15;13(6):e15663. Epub 2021 Jun 15.

1st Department of Obstetrics and Gynecology, "Alexandra" General Hospital, National and Kapodistrian University of Athens, Medical School, Athens, GRC.

A variety of protocols have evaluated the use of several forms of gonadotropins in controlled ovarian stimulation (COS). We aim to review the evolving trends on the use of gonadotropins human chorionic gonadotropin (hCG), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) over time and their combinations in COS for patients who undergo assisted reproductive techniques (ART) protocols. A meticulous search of three electronic databases was performed for articles published in the field up to September 2020. Read More

View Article and Full-Text PDF

Effects of exercise training with blood flow restriction on vascular function in adults: a systematic review and meta-analysis.

PeerJ 2021 7;9:e11554. Epub 2021 Jul 7.

Innovation, IMPlementation And Clinical Translation in Health (IIMPACT), University of South Australia, Adelaide, South Australia, Australia.

Background: Blood flow restricted exercise (BFRE) improves physical fitness, with theorized positive effects on vascular function. This systematic review and meta-analysis aimed to report (1) the effects of BFRE on vascular function in adults with or without chronic health conditions, and (2) adverse events and adherence reported for BFRE.

Methodology: Five electronic databases were searched by two researchers independently to identify studies reporting vascular outcomes following BFRE in adults with and without chronic conditions. Read More

View Article and Full-Text PDF