561,170 results match your criteria proteolytic enzymes

Metabolic changes in synovial cells in early inflammation: Involvement of CREB phosphorylation in the anti-inflammatory effect of 2-deoxyglucose.

Arch Biochem Biophys 2021 Jun 8:108962. Epub 2021 Jun 8.

Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.

The involvement of metabolic reprogramming has been suggested to contribute to the pathophysiology of rheumatoid arthritis (RA). Glycolysis is enhanced in synovial cell metabolism in RA patients. Inhibitors of glycolysis are known to have anti-inflammatory effects. Read More

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The bile salt content of human bile impacts on simulated intestinal proteolysis of β-lactoglobulin.

Food Res Int 2021 Jul 12;145:110413. Epub 2021 May 12.

Gdańsk University of Technology, Faculty of Chemistry, Department of Colloid and Lipid Science, Gabriela Narutowicza 11/12, 80-322 Gdańsk, Poland. Electronic address:

The gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity of proteolytic enzymes. Human bile has not been considered to facilitate proteolysis in the small intestine, but rather to assist in intestinal lipolysis. However, human bile can potentially influence proteins that are largely resistant to gastric digestion, and which are mainly hydrolysed after they have been transferred to the small intestine. Read More

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Triticum monococcum amylase trypsin inhibitors possess a reduced potential to elicit innate immune response in celiac patients compared to Triticum aestivum.

Food Res Int 2021 Jul 7;145:110386. Epub 2021 May 7.

Institute of Food Sciences, CNR, Avellino, Italy; E.L.F.I.D, University "Federico II" Napoli, Italy. Electronic address:

Scope: Several studies reported a role of amylase/trypsin-inhibitors (ATIs) of common wheat species in promoting immune reactions. Here, we investigated in celiac disease (CD), the immunogenic properties of ATIs from diploid compared to common hexaploid wheats after an in vitro proteolytic hydrolysis.

Methods And Results: ATIs purified from two lines of diploid Triticum monococcum (TM), Monlis and Norberto-ID331, and from Triticum aestivum (TA), Sagittario, were digested with pepsin-chymotrypsin (PC) enzymes and analyzed using a proteomic approach, and subsequently their immune stimulatory properties were investigated on jejunal biopsies and T-cell lines from CD patients. Read More

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Induction and Repression of Hydrolase Genes in .

Front Microbiol 2021 24;12:677603. Epub 2021 May 24.

Laboratory of Fermentation Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.

The filamentous fungus , also known as yellow mold, produces high levels of hydrolases such as amylolytic and proteolytic enzymes. This property of producing large amounts of hydrolases is one of the reasons why has been used in the production of traditional Japanese fermented foods and beverages. A wide variety of hydrolases produced by have been used in the food industry. Read More

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[Thrombotic thrombocytopenic purpura].

Rinsho Ketsueki 2021 ;62(5):430-434

Saitama Medical University Hospital.

In this article, I mainly review the molecular targeted therapy for thrombotic thrombocytopenic purpura (TTP). TTP is one of thrombotic microangiopathies (TMA), which demonstrate hemolytic anemia with red blood cell destruction and thrombocytopenia. Another TTP, hemolytic uremic anemia (HUS) reveals bloody stood and acute kidney failure. Read More

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[A Case of Ureteral Metastasis from Prostate Cancer].

Hinyokika Kiyo 2021 Apr;67(4):171-176

The Department of Urology, Kobe City Nishi-Kobe Medical Center.

Ureteral metastasis from prostate cancer is rare. The present case report describes an 83-year-old patient with distant metastasis of prostate cancer to the right ureter that caused hydronephrosis. Upon initial examination at our hospital, he presented with a high prostate-specific antigen (PSA) level of 10. Read More

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[Thrombolysis with Tenecteplase in a stroke with a long time window after onset of symptoms. Report of one case].

Rev Med Chil 2021 Jan;149(1):128-131

Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.

We report a 78-year-old man with a basal Rankin score of 2 points, last seen 10 hours before in good conditions, who arrived at the emergency department with left hemiparesis, hypoesthesia, and spacial neglect. Neuroimaging was compatible with stroke in the territory of the right middle cerebral artery. Due to the evolution time of the stroke, usual thrombolysis was contraindicated. Read More

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January 2021

Botulinum toxin type A and acupuncture for masticatory myofascial pain: a randomized clinical trial.

J Appl Oral Sci 2021 4;29:e20201035. Epub 2021 Jun 4.

Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Departamento de Prótese e Periodontia, Piracicaba, SP, Brasil.

Background: BoNT-A has been widely used for TMD therapy. However, the potential benefits compared to dry needling techniques are not clear.

Objective: this study aimed to compare the immediate effects of botulinum toxin type A (BoNT-A) injections and Acupuncture in myofascial temporomandibular disorders (TMD) patients. Read More

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Carangoides bartholomaei (Cuvier, 1833) stomach: a source of aspartic proteases for industrial and biotechnological applications.

Braz J Biol 2021 4;82:e234413. Epub 2021 Jun 4.

Universidade Federal da Paraíba - UFPB, Centro de Ciências Exatas e da Natureza, Departamento de Biologia Molecular, Laboratório de Biomoléculas de Organismos Aquáticos, João Pessoa, PB, Brasil.

The viscera and other residues from fish processing are commonly discarded by the fishing industry. These by-products can be a source of digestive enzymes with industrial and biotechnological potential. In this study, we aimed at the extraction, characterization, and application of acidic proteases from the stomach of Carangoides bartholomaei (Cuvier, 1833). Read More

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[The Effect of γ-secretase Inhibitor Combined with BMSC on the aGVHD in Mice Model].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):937-943

Department of Hematology, Taian City Central Hospital, Shandong First Medical University, Taian 271000, Shandong Province, China,E-mail:

Objective: To establish the aGVHD mouse model,and investigate the regulatory effect and its mechanism of low-dose GSI combined with BMSC on aGVHD mice.

Methods: C57BL/6 (H-2b) and BALB/c (H-2d) were selected as donor and recipient of allogeneic transplantation to establish the aGVHD mouse model. BALB/c mice were randomly divided into 6 groups, which were the bone marrow cell infusion after irradiation (BM) group; the bone marrow cells + spleen cells after irradiation (BM+SC) group; the bone marrow cells + spleen cells + DMSO (BM+SC+DMSO) (transplant control) group; bone marrow cells + splenocytes +GSI after irradiation (BM+SC+GSI) group; bone marrow cells + spleen cells + bone marrow mesenchymal stromal infusion after irradiation cell (BM+SC+BMSC) group; bone marrow cells + spleen cells + bone marrow mesenchymal stromal cells +GSI infused after irradiation (BM+SC+BMSC+GSI) group. Read More

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[Biological Function of CysR Domain of ADAMTS13].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):893-900

Department of Hematology,Yanbian University Hospital (Yanbian Hospital), Yanji 133000, Jilin Province, China,E-mail:

Objective: To investigate the biological function of Cysteine rich (CysR) domain of a disintegrin and metalloprotease with thrombospondin type 1 repeats-13 (ADAMTS13) on cleavage of von Willebrand factor (vWF) and provide experimental evidence for exploring the pathogenesis of thrombotic thrombocytopenic purpura (TTP).

Methods: The six amino acids (EDGTLS) in ADAMTS13 CysR domain were point mutated one by one, and the mutant ADAMTS13 proteins were expressed and purified. The cleavage products of vWF polymer by wild-type or mutant ADAMTS13 under denaturing condition or shear stress were separated by 1% SeaKem HGT agarose gel and detected by Western blot. Read More

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Zeolitic Imidazolate Frameworks (ZIF-8) for Biomedical Applications: A Review.

Curr Med Chem 2021 Jun 8. Epub 2021 Jun 8.

Advanced Multifunctional Materials Laboratory, Department of Chemistry, Assiut University, Assiut, 71516, Egypt.

Beyond being an excellent protective material for bioentities, zeolitic imidazolate frameworks (ZIF-8) have advanced several applications, including biomedical applications. The straightforward synthesis of ZIF-8 at mild conditions improved the biomineralization of several biomolecules, e.g. Read More

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Puerarin protects against human retinal endothelial cells injury induced by high glucose via regulating miR-16-5p/CASP1 axis.

Gen Physiol Biophys 2021 May;40(3):235-243

Department of Ophthalmology, The Affiliated Hospital to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.

We aimed to investigate the effects of puerarin on high glucose (HG)-induced human retinal endothelial cells (HRECs) injury. Cells were exposed to puerarin in the presence or absence of HG challenge. Cell viability was determined using a CCK-8 assay. Read More

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[Functions of E3 Ubiquitin Ligase Hyd in Drosophila Tissues].

Mol Biol (Mosk) 2021 May-Jun;55(3):355-361

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.

The ubiquitin-proteasome system (UPS) is an important regulator of the main cellular processes. The components of the UPS are involved in the regulation of the cell cycle, signal transduction, the cell response to DNA damage, metabolism, and transcription control. E3 ubiquitin ligases (the enzymes that covalently attaches ubiquitin to target proteins) play a key role in the functioning of the UPS. Read More

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Targeting SNARE-Mediated Vesicle Transport to Block Invadopodium-Based Cancer Cell Invasion.

Front Oncol 2021 21;11:679955. Epub 2021 May 21.

Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.

During metastasis, cancer cells can invade extracellular matrix (ECM) through a process mediated by matrix-degrading protrusions of the plasma membrane, termed invadopodia. Formation of invadopodia correlates with cells' invasive and metastatic potential, and thus presents a potential target for therapeutic approaches to target metastatic progression. Invadopodia formation is dependent on the recruitment of proteins involved in intracellular signaling, actin cytoskeleton remodeling, and proteolytic matrix modification. Read More

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An Undiagnosed Case of Chronic Pancreatitis With Multiple Visceral Arteries Pseudoaneurysm.

Cureus 2021 May 1;13(5):e14789. Epub 2021 May 1.

General Medicine, Rehman Medical Institute, Peshawar, PAK.

Visceral artery aneurysms, which could be either true or pseudo, are abnormal focal dilations of vessels supplying the abdominal organs. True aneurysms, by definition, suggest dilation of the vessel in response to increased blood flow, ultimately causing a blood-filled sac to form. Pseudoaneurysm, however, is the pooling of blood in surrounding tissues secondary to trauma or rupture. Read More

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COVID-19 in normal, diseased and transplanted liver.

World J Gastroenterol 2021 May;27(20):2576-2585

Hepatology Unit, Department of Medicine, University of Tor Vergata, Rome 00133, Italy.

Starting from December 2019 the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has extended in the entire world giving origin to a pandemic. Although the respiratory system is the main apparatus involved by the infection, several other organs may suffer coronavirus disease 2019 (COVID-19)-related injuries. The human tissues expressing angiotensin-converting enzyme 2 (ACE2) are all possible targets of viral damage. Read More

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Mucin 5AC is significantly upregulated in exosomes from the nasal lavage fluid and may promote the expression of COX-2, VEGF and MMP-9: an implication in nasal polyp pathogenesis.

Rhinology 2021 Jun;59(3):328-336

Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan; Center for Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan.

Background: Exosomes are critical mediators of intercellular communication and could be involved in many human diseases; however, little is known about the role of exosomes in nasal polyps (NP).

Methods: Exosomes in nasal lavage fluids (NLF) were isolated by ultracentrifugation. Exosome identity was validated by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and specific exosomal markers. Read More

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The effect of coronaviruses on olfaction: systematic review.

Rhinology 2021 Jun;59(3):226-235

Department of Otorhinolaryngology, Amsterdam University Medical Centres, location AMC, Amsterdam, The Netherlands.

Background: Unlike other respiratory viruses, SARS-CoV-2 causes anosmia without sinonasal inflammation. Here we systematically review the effects of the 7 known human coronaviruses on olfaction to determine if SARS-CoV-2 distinctly affects the olfactory system.

Method: PubMed, EMBASE, Web of Science, bioRxiv, medRxiv and DOAJ were searched for studies describing pathophysiological, immunohistochemical, cytological and clinical data. Read More

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Heterologous expression and characterisation of a keratinase produced byChryseobacterium carnipullorum.

Protein Expr Purif 2021 Jun 3:105926. Epub 2021 Jun 3.

Department of Microbiology and Biochemistry, PO Box 339, University of the Free State, Bloemfontein, Free State, South Africa. Electronic address:

Chryseobacterium carnipullorum 9_R23581, isolated from raw chicken meat, was evaluated for its potential to degrade keratin found in feathers. The focus of this study was to heterologously express and characterise a keratinolytic enzyme produced by C. carnipullorum. Read More

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Multiple thrombosis associated with Cytomegalovirus enterocolitis in an immunocompetent patient: a case report.

BMC Infect Dis 2021 Jun 5;21(1):530. Epub 2021 Jun 5.

Department of Internal Medicine, Hyogo Prefectural Tamba Medical Center, 2002-7 Iso, Hikami-cho, Tamba, 669-3495, Japan.

Background: Cytomegalovirus (CMV) is reported to have thrombogenic characteristics that activate factor X in vitro and stimulate the production of factor VIII and von Willebrand factor (vWF). Thrombosis associated with CMV infection is prevalent among immunocompromised patients and predominantly presents as a solitary large thrombus in the deep vein, pulmonary artery, splanchnic arteriovenous ducts, or other similar sites. Multiple thrombi, however, are rarely observed in such cases. Read More

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USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

Sci Total Environ 2021 Aug 9;783:146898. Epub 2021 Apr 9.

Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009, China.

Di-n-butylphthalate (DBP) has been listed as an environmental priority pollutant in China due to its distinct biotoxicity. Epidemiological studies have shown that exposure to DBP is closely related to a series of congenital and acquired defects in the male reproductive system. The oxidative stress injury caused by DBP plays an important role in these defects. Read More

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Expression of the SARS-CoV-2 Receptor ACE2 in Human Retina and Diabetes-Implications for Retinopathy.

Invest Ophthalmol Vis Sci 2021 06;62(7)

Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States.

Purpose: To investigate the expression of angiotensin-converting enzyme 2 (ACE2), the receptor for SARS-CoV-2 in human retina.

Methods: Human post-mortem eyes from 13 non-diabetic control cases and 11 diabetic retinopathy cases were analyzed for the expression of ACE2. To compare the vascular ACE2 expression between different organs that involve in diabetes, the expression of ACE2 was investigated in renal specimens from nondiabetic and diabetic nephropathy patients. Read More

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Angiotensin-converting enzyme-1 gene insertion/deletion polymorphism may be associated with COVID-19 clinical severity: a prospective cohort study.

Ann Saudi Med 2021 May-Jun;41(3):141-146. Epub 2021 Jun 1.

From the Department of Internal Medicine, Section of Rheumatology, Samsun Education and Research Hospital, Samsun, Turkey.

Background: Angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism may play a role in the pathogenesis of coronavirus-19 disease (COVID-19).

Objectives: Investigate the relationship between ACE I/D polymorphism and the clinical severity of COVID-19.

Design: Prospective cohort study. Read More

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[Effect of electroacupuncture at "Weizhong" (BL40) on expression of muscular PDGF-CC, PDGFR-α and MMP-1 in rats with lumbar multifidus muscle injury].

Zhen Ci Yan Jiu 2021 May;46(5):397-403

Department of Acupuncture and Rehabilitation, Guangdong Second Hospital of Traditional Chinese Medicine, Guangzhou 510095.

Objective: To observe the effect of electroacupuncture (EA) of "Weizhong" (BL40) on the expression of platelet-derived growth factor (PDGF)-CC, PDGF receptor (PDGFR)α and matrix metalloproteinase-1 (MMP-1) in rats with lumbar multifidus muscle injury (LMMI) so as to study its mechanisms underlying improvement of skeletal muscle injury.

Methods: Fifty-four male SD rats were randomly divided into normal group (=6), model group (=24) and EA group (=24), and the latter two groups were further divided into four subgroups (1, 3, 5 and 7 days), with 6 rats in each group. The LMMI model was established by injection of 0. Read More

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Addition of lactoferrin and substance P in a chitin/PLGA-CaSO hydrogel for regeneration of calvarial bone defects.

Mater Sci Eng C Mater Biol Appl 2021 Jul 6;126:112172. Epub 2021 May 6.

Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi-682041, India. Electronic address:

Calcium-based injectable hydrogels with various bioactive active molecules possess a great potential for bone regeneration. Herein, we have synthesized a chitin-PLGA-calcium sulfate hydrogel (CSG) containing bioactive molecules - lactoferrin (LF) and substance P (SP). SEM and XRD analysis revealed that CS crystal growth was altered with the addition of LF. Read More

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Three-month early change in prostate-specific antigen levels as a predictive marker for overall survival during hormonal therapy for metastatic hormone-sensitive prostate cancer.

BMC Res Notes 2021 Jun 3;14(1):227. Epub 2021 Jun 3.

Department of Urology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa, 903-0215, Japan.

Objective: To date, there are no useful markers for predicting the prognosis of metastatic hormone-sensitive prostate cancer (mHSPC). We evaluated the effect of early changes in prostate-specific antigen (PSA) levels after androgen deprivation therapy (ADT) on castration-resistant prostate cancer (CRPC) progression and overall survival (OS) in mHSPC patients.

Results: In 71 primary mHSPC patients treated with ADT, the median times to CRPC and OS were 15 months and 92 months, respectively. Read More

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[pplication of Asn-Gly-Arg sequence based cyclic peptides for targeted tumor therapy].

Magy Onkol 2021 Jun 15;65(2):113-120. Epub 2021 May 15.

Kísérletes Farmakológiai Osztály, Országos Onkológiai Intézet, Budapest, Hungary.

The in vivo antitumor effect of two NGR sequence containing peptide-daunomycin conjugates was studied on CD13+ Kaposi's sarcoma s.c. tumor model on SCID mice, and on orthotopically developed CD13- HT-29 colon adenocarcinoma SCID mouse model. Read More

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[Active surveillance for patients with localized prostate cáncer. Adherence and protocol drop analysis.]

Arch Esp Urol 2021 Jun;74(5):477-487

Grupo de Investigación Traslacional de Urología (GITUR-IBSAL). Instituto de Investigación Biomédica de Salamanca. España. Servicio de Urología. Complejo Asistencial Universitario de Salamanca. España.

Objective: VA is currently considered the treatment of choice for patients with low and very low risk prostate cancer. We analyzed the evolution of this treatment strategy in our series and adherence to the protocol.

Material And Methods: Ambispective study of patients in VA in our center between 2014- 2019. Read More

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UBE4B, a microRNA-9 target gene, promotes autophagy-mediated Tau degradation.

Nat Commun 2021 06 2;12(1):3291. Epub 2021 Jun 2.

Metabolism and Neurophysiology Research Group, KRIBB, Daejeon, Korea.

The formation of hyperphosphorylated intracellular Tau tangles in the brain is a hallmark of Alzheimer's disease (AD). Tau hyperphosphorylation destabilizes microtubules, promoting neurodegeneration in AD patients. To identify suppressors of tau-mediated AD, we perform a screen using a microRNA (miR) library in Drosophila and identify the miR-9 family as suppressors of human tau overexpression phenotypes. Read More

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