630,322 results match your criteria proteins involved


Genetic and pharmacological inhibition of fatty acid-binding protein 4 alleviated inflammation and early fibrosis after toxin induced kidney injury.

Int Immunopharmacol 2021 May 12;96:107760. Epub 2021 May 12.

Kidney Research Institute, Division of Nephrology, West China Hospital of Sichuan University, Chengdu 610041, China.

Considerable data have suggested that acute kidney injury (AKI) is often incompletely repaired and could lead to chronic kidney disease (CKD). As we known, toxin-induced nephropathy triggers the rapid production of proinflammatory mediators and the prolonged inflammation allows the injured kidneys to develop interstitial fibrosis. In our previous study, fatty acid-binding protein 4 (Fabp4) has been reported to be involved in the process of AKI. Read More

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MiR-128-3p suppresses tumor proliferation and metastasis via targeting CDC6 in hepatocellular carcinoma cells.

Tissue Cell 2021 Mar 23;72:101534. Epub 2021 Mar 23.

School of Medicine, Shaoxing University, China; Department of General Surgery, Xinchang People's Hospital, China. Electronic address:

Background: MicroRNAs (miRNAs) are known to be involved in the pathogenesis of various cancers. The present study devotes efforts to discover the role of miR-128-3p in hepatocellular carcinoma (HCC).

Methods: MiR-128-3p and cell division cycle 6 (CDC6) expressions in HCC tissue (n = 50) and adjacent normal tissue (n = 50) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Read More

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Proteomic insights into protostane triterpene biosynthesis regulatory mechanism after MeJA treatment in Alisma orientale (Sam.) Juz.

Biochim Biophys Acta Proteins Proteom 2021 May 12:140671. Epub 2021 May 12.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Protostane triterpenes in Alisma orientale (Sam.) Juz. have unique structural features with distinct pharmacological activities. Read More

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β-Glucan: A dual regulator of apoptosis and cell proliferation.

Int J Biol Macromol 2021 May 12. Epub 2021 May 12.

Department of Biotechnology, University of Kashmir, Srinagar 190006, J&K, India. Electronic address:

β-Glucans are polysaccharides generally obtained from the cell wall of bacteria, fungi, yeasts, and aleurone layer of cereals. β-Glucans are polymers, with β-1,3 glucose as core linear structure, but they differ in their main branch length, linkages, and branching patterns, giving rise to high and low-molecular-weight β-glucans. They are well-known cell response modifiers with immune-modulating, nutraceutical, and health beneficial effects, including anticancer and pro-apoptotic properties. Read More

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Proteomic Analysis of the Responses to Co-Stimulation of Intestinal Aeromonas and Shewanella in Lamprey Leukocytes.

Curr Microbiol 2021 May 15. Epub 2021 May 15.

School of Life Science, Liaoning Normal University, Dalian, China.

Lamprey, one of the most basal jawless vertebrate, is an excellent model for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. This study investigated the differentially expressed proteins in lamprey leukocytes in response to the co-stimulation of intestinal Aeromonas and Shewanella by using quantitative proteomics techniques. Significant differentially expressed proteins were identified. Read More

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Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells.

Proteomics 2021 May 15:e2000098. Epub 2021 May 15.

Cancer Inflammation Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, VIC, Australia.

Doublecortin-like kinase 1 (DCLK1) is a putative cancer stem cell marker, a promising diagnostic and prognostic maker for malignant tumors and a proposed driver gene for gastric cancer (GC). DCLK1 overexpression in a majority of solid cancers correlates with lymph node metastases, advanced disease and overall poor-prognosis. In cancer cells, DCLK1 expression has been shown to promote epithelial-to-mesenchymal transition (EMT), driving disruption of cell-cell adhesion, cell migration and invasion. Read More

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PP1, PKA and DARPP-32 in breast cancer: A retrospective assessment of protein and mRNA expression.

J Cell Mol Med 2021 May 15. Epub 2021 May 15.

Division of Cancer and Stem Cells, Nottingham Breast Cancer Research Centre, School of Medicine, University of Nottingham Biodiscovery Institute, Nottingham, UK.

Cyclic AMP-dependent protein kinase A (PKA) and protein phosphatase 1 (PP1) are proteins involved in numerous essential signalling pathways that modulate physiological and pathological functions. Both PP1 and PKA can be inhibited by dopamine- and cAMP-regulated phosphoprotein 32 kD (DARPP-32). Using immunohistochemistry, PKA and PP1 expression was determined in a large primary breast tumour cohort to evaluate associations between clinical outcome and clinicopathological criteria (n > 1100). Read More

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ATP regulates RNA-driven CIRBP phase separation.

Protein Sci 2021 May 14. Epub 2021 May 14.

Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology & Biochemistry, Medical University of Graz, Austria.

Intrinsically disordered proteins (IDPs) and proteins containing intrinsically disordered regions (IDRs) are highly abundant in the proteome of eukaryotes and are extensively involved in essential biological functions. More recently their role in the organization of biomolecular condensates has become evident and along with their misregulation in several neurologic disorders. Currently, most studies involving these proteins are carried out in vitro and using purified proteins. Read More

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Dual activity of anthocyanidin reductase supports the dominant plant proanthocyanidin extension unit pathway.

Sci Adv 2021 May 14;7(20). Epub 2021 May 14.

BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA.

Proanthocyanidins (PAs) are plant natural products important for agriculture and human health. They are polymers of flavan-3-ol subunits, commonly (-)-epicatechin and/or (+)-catechin, but the source of the in planta extension unit that comprises the bulk of the polymer remains unclear, as does how PA composition is determined in different plant species. Anthocyanidin reductase (ANR) can generate 2,3--epicatechin as a PA starter unit from cyanidin, which itself arises from 2,3--leucocyanidin, but ANR proteins from different species produce mixtures of flavan-3-ols with different stereochemistries in vitro. Read More

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Mannose and mannobiose specific responses of insect associated cellulolytic .

Appl Environ Microbiol 2021 May 14. Epub 2021 May 14.

Graduate School of Global Food Resources, Hokkaido University, Sapporo, 060-0809, Japan

The cellulolytic insect symbiont bacterium, sp. SirexAA-E (SirexAA-E) secretes a suite of Carbohydrate Active enZymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources. Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components. Read More

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Propionate production from carbon monoxide by synthetic co-cultures of spp. and propionigenic bacteria.

Appl Environ Microbiol 2021 May 14. Epub 2021 May 14.

Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands

Gas fermentation is a promising way for converting CO-rich gases to chemicals. We studied the use of synthetic co-cultures composed of carboxydotrophic and propionigenic bacteria to convert CO to propionate. So far isolated carboxydotrophs cannot directly ferment CO to propionate, and therefore this co-cultivation approach was investigated. Read More

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Upregulated Talin1 synergistically boosts β-estradiol-induced proliferation and pro-angiogenesis of eutopic and ectopic endometrial stromal cells in adenomyosis.

Reprod Biol Endocrinol 2021 May 14;19(1):70. Epub 2021 May 14.

Department of Minimally Invasive Gynecologic Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, No.17 Qi Helou Road, Dong Cheng District, Beijing, 100006, China.

Adenomyosis (ADS) is an estrogen-dependent gynecological disease with unspecified etiopathogenesis. Local hyperestrogenism may serve a key role in contributing to the origin of ADS. Talin1 is mostly identified to be overexpressed and involved in the progression of numerous human carcinomas through mediating cell proliferation, adhesion and motility. Read More

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HSP90-dependent PUS7 overexpression facilitates the metastasis of colorectal cancer cells by regulating LASP1 abundance.

J Exp Clin Cancer Res 2021 May 14;40(1):170. Epub 2021 May 14.

State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 710032, Xi'an, Shaanxi Province, China.

Background: Pseudouridine synthase (PUS) 7 is a member of the PUS family that catalyses pseudouridine formation. It has been shown to be involved in intellectual development and haematological malignancies. Nevertheless, the role and the underlying molecular mechanisms of PUS7 in solid tumours, such as colorectal cancer (CRC), remain unexplored. Read More

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Genome-informed approach to identify genetic determinants of Flavobacterium psychrophilum phage susceptibility.

Environ Microbiol 2021 May 14. Epub 2021 May 14.

Marine Biological Section, Department of Biology, University of Copenhagen, Helsingør, Denmark.

The fish pathogen Flavobacterium psychrophilum infects farmed salmonids worldwide, and application of bacteriophages has been suggested for controlling disease outbreaks in aquaculture. Successful application of phages requires detailed knowledge about the variability in phage susceptibility of the host communities. In this study we analyzed the genetic diversity of F. Read More

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Zinc limitation triggers anticipatory adaptations in Mycobacterium tuberculosis.

PLoS Pathog 2021 May 14;17(5):e1009570. Epub 2021 May 14.

School of Life Sciences, University of Hawai'i at Mānoa, Honolulu, Hawaii, United States of America.

Mycobacterium tuberculosis (Mtb) has complex and dynamic interactions with the human host, and subpopulations of Mtb that emerge during infection can influence disease outcomes. This study implicates zinc ion (Zn2+) availability as a likely driver of bacterial phenotypic heterogeneity in vivo. Zn2+ sequestration is part of "nutritional immunity", where the immune system limits micronutrients to control pathogen growth, but this defense mechanism seems to be ineffective in controlling Mtb infection. Read More

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Graphene Oxide Nanosheets Interact and Interfere with SARS-CoV-2 Surface Proteins and Cell Receptors to Inhibit Infectivity.

Small 2021 May 14:e2101483. Epub 2021 May 14.

Stem Cell Institute, Ankara University, Balgat, Ankara, 06520, Turkey.

Nanotechnology can offer a number of options against coronavirus disease 2019 (COVID-19) acting both extracellularly and intracellularly to the host cells. Here, the aim is to explore graphene oxide (GO), the most studied 2D nanomaterial in biomedical applications, as a nanoscale platform for interaction with SARS-CoV-2. Molecular docking analyses of GO sheets on interaction with three different structures: SARS-CoV-2 viral spike (open state - 6VYB or closed state - 6VXX), ACE2 (1R42), and the ACE2-bound spike complex (6M0J) are performed. Read More

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FSH modulated cartilage ECM metabolism by targeting the PKA/CREB/SOX9 pathway.

J Bone Miner Metab 2021 May 14. Epub 2021 May 14.

School of Medicine, Shandong University, jinan, 250021, shandong, China.

Introduction: Osteoarthritis (OA) is a common joint disease characterized by articular cartilage degeneration. The prevalence of OA is higher among women than men, and this prevalence is closely related to menopause. The classic view assumes that the underlying mechanism of postmenopausal OA is attributed to declining estrogen levels. Read More

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Synthesis of a biotinylated heptose 1,7-bisphosphate analogue, a probe to study immunity and inflammation.

Org Biomol Chem 2021 May 14. Epub 2021 May 14.

University of Namur (UNamur), NARILIS, Department of Chemistry, rue de Bruxelles 61, 5000 Namur, Belgium.

d-glycero-d-manno-Heptose-1β,7-bisphosphate (HBP) is a bacterial metabolite that can induce a TIFA-dependent innate immune response in mammals. It was recently discovered that after HBP enters into the cytoplasm of the host cell, it is transformed into ADP-heptose-7-phosphate, which then leads to ALPK1-TIFA-dependent inflammatory response. In order to provide a molecular tool allowing the discovery of the proteins involved in this novel inflammatory pathway, we designed and synthesized a biotinylated analogue of HBP. Read More

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Repurposing of the herbal formulations: molecular docking and molecular dynamics simulation studies to validate the efficacy of phytocompounds against SARS-CoV-2 proteins.

J Biomol Struct Dyn 2021 May 14:1-15. Epub 2021 May 14.

Gujarat State Biotechnology Mission, Gandhinagar, Gujarat, India.

Herbal formulations mentioned in traditional medicinal texts were investigated for effect against SARS-COV-2 proteins involved in various functions of a virus such as attachment, entry, replication, transcription, etc. To repurpose and validate polyherbal formulations, molecular docking was performed to study the interactions of more than 150 compounds from various formulations against the SARS-CoV-2 proteins. Molecular dynamics (MD) simulation was performed to evaluate the interaction of top scored ligands with the various receptor proteins. Read More

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Metformin Carbon Dots for Promoting Periodontal Bone Regeneration via Activation of ERK/AMPK Pathway.

Adv Healthc Mater 2021 May 14:e2100196. Epub 2021 May 14.

Hospital of Stomatology, Jilin University, Changchun, 130021, P. R. China.

The osteogenic potential of mesenchymal stem cells (MSCs) is severely impaired under persistent inflammation of periodontitis. A highly efficient way to promote or rescue osteogenic potential of MSCs under inflammation remains an unmet goal. Herein, metformin carbon dots (MCDs) with excellent biocompatibility are prepared from metformin hydrochloride and citric acid via a hydrothermal method. Read More

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Mapping of the amniotic fluid proteome of fetuses with congenital anomalies of the kidney and urinary tract identifies Plastin 3 as a protein involved in glomerular integrity.

J Pathol 2021 May 13. Epub 2021 May 13.

Institut National de la Santé et de la Recherche Médicale (INSERM), U1297, Institut of Cardiovascular and Metabolic Disease, Toulouse, France.

Congenital anomalies of the kidney and the urinary tract (CAKUT) are the first cause of chronic kidney disease in childhood. Several genetic and environmental origins are associated with CAKUT, but most pathogenic pathways remain elusive. Considering the amniotic fluid (AF) composition as a proxy for fetal kidney development, we analyzed the AF proteome from non-severe CAKUT (n=19), severe CAKUT (n=14), and healthy control (n=22) fetuses using LC-MS/MS. Read More

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Gm40600 promotes CD4 T cell responses by interacting with Ahnak.

Immunology 2021 May 13. Epub 2021 May 13.

Beijing Institute of Brain Disorders, Laboratory of Brain Disorders, Ministry of Science and Technology, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing, 100069, China.

It is important to characterize novel proteins involved in T- and B-cell responses. Our previous study demonstrated that a novel protein, Mus musculus Gm40600, reduced the proliferation of Mus musculus plasmablast (PB)-like SP 2/0 cells and B-cell responses induced in vitro by LPS. In the present study, we revealed that Gm40600 directly promoted CD4 T cell responses to indirectly up-regulate B-cell responses. Read More

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TMT-labeled quantitative proteomic analysis to identify proteins associated with the stability of peanut milk.

J Sci Food Agric 2021 May 14. Epub 2021 May 14.

Institute of Agricultural and Sideline Products Processing, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.

Background: Peanut milk benefits human health mainly due to its high protein content and suitable amino acid composition. To reveal the molecular mechanism affecting the quality of peanut milk, tandem mass tag (TMT)-labeled proteomic analysis was applied to identify the proteome variation between two peanut cultivars that produced peanut milk with the best and worst stability.

Results: A total of 478 differentially abundant proteins (fold change>1. Read More

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Proteomic insights into the photosynthetic divergence between bark and leaf chloroplasts in Salix matsudana.

Tree Physiol 2021 May 13. Epub 2021 May 13.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry and grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, PR China.

Bark chloroplasts play important roles in carbon balancing by recycling internal stem CO2 into assimilated carbon. The photosynthetic response of bark chloroplasts to interior stem environments has been studied recently in woody plants. However, the molecular regulatory mechanisms underlying specific characteristics of bark photosynthesis remain unclear. Read More

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SET knockdown attenuated phenotype modulation and calcium channel associated markers of airway smooth muscle cells in asthmatic mice.

Ann Transl Med 2021 Apr;9(8):657

Key Laboratory of Shenzhen Respiratory Disease, Shenzhen Institute of Respiratory Disease, Shenzhen People's Hospital (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University), Shenzhen, China.

Background: Dysfunctional phenotype modulation and calcium channels in airway smooth muscle cells (ASMCs) are important characteristics of airway remodeling in chronic asthma. However, the mechanisms underlying these pathological processes remain unclear. SET (I2PP2A, inhibitor-2 of protein phosphatase 2A) has many significant functions and is involved in various physiological and pathological processes. Read More

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Adenosine A3 receptor activated in HO oxidative stress of primary open-angle glaucoma.

Ann Transl Med 2021 Apr;9(7):526

Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, China.

Background: Primary open-angle glaucoma (POAG), as one of the leading reasons for blindness, is mainly due to trabecular meshwork (TM) dysfunction. Bioinformatics analysis was used to find related genes involved in TM oxidative stress, which is a major cause of TM fibrosis.

Methods: A total of three datasets from the Gene Expression Omnibus (GEO) database were used to identify differentially expressed genes (DEGs). Read More

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Serum Cytokine Profiling Identifies Axl as a New Biomarker Candidate for Active Eosinophilic Granulomatosis With Polyangiitis.

Front Mol Biosci 2021 27;8:653461. Epub 2021 Apr 27.

Department of Pathophysiology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.

Eosinophilic granulomatosis with polyangiitis (EGPA) prognosis is generally favorable and is treated with combined corticosteroids/immunosuppressor(s) therapy. However, disease flares increase the number of clinical visits. Therefore, discovering new serum biomarkers for early identification of active EGPA is crucial. Read More

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A Toll-like receptor identified in and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria.

PeerJ 2021 30;9:e11282. Epub 2021 Apr 30.

Center of Deep Sea Research and Key Laboratory of Marine Ecology & Environmental Sciences (CODR and KLMEES), Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Symbiosis with chemosynthetic bacteria is an important ecological strategy for the deep-sea megafaunas including mollusks, tubeworms and crustacean to obtain nutrients in hydrothermal vents and cold seeps. How the megafaunas recognize symbionts and establish the symbiosis has attracted much attention. Bathymodiolinae mussels are endemic species in both hydrothermal vents and cold seeps while the immune recognition mechanism underlying the symbiosis is not well understood due to the nonculturable symbionts. Read More

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Anti-VEGF treatment suppresses remodeling factors and restores epithelial barrier function through the E-cadherin/β-catenin signaling axis in experimental asthma models.

Exp Ther Med 2021 Jul 29;22(1):689. Epub 2021 Apr 29.

Department of Pediatric Allergy and Immunology, Celal Bayar University Medical Faculty, Manisa 45030, Turkey.

Besides maintaining a physical barrier with adherens junctional (AJ) and tight junctional proteins, airway epithelial cells have important roles in modulating the inflammatory processes of allergic asthma. E-cadherin and β-catenin are the key AJ proteins that are involved in airway remodeling. Various mediators such as transforming growth factor-β (TGF-β), epidermal growth factor (EGF), fibroblast growth factor (FGF), platelet derived growth factor (PDGF), insulin-like growth factor (IGF), tumor necrosis factor-α (TNF-α) and angiogenic factors, such as vascular endothelial growth factor (VEGF), are released by the airway epithelium in allergic asthma. Read More

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Acute glucose fluctuation promotes intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway.

Exp Ther Med 2021 Jul 28;22(1):688. Epub 2021 Apr 28.

Department of Gastroenterology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210000, P.R. China.

High blood glucose commonly occurs in patients with diabetes mellitus, but little is known of its effects on intestinal epithelial cells, or its associated mechanisms of action therein. In the present study, intestinal epithelial cells were assigned to five groups: i) The normal glucose (NG) group, incubated in 5.0 mmol/l glucose; ii) the constant high glucose (CHG) group, treated with 25. Read More

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