46,699 results match your criteria protein resources


Ultrafiltration strategy combined with nanoLC-MS/MS based proteomics for monitoring potential residual proteins in TCMIs.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 May 30;1178:122818. Epub 2021 May 30.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address:

Traditional Chinese medicine injections (TCMIs) containing complex constituents frequently cause unpredictable adverse reactions. The residual heterologous proteins in TCMIs may be one kind of the sensitized constituents. However, few methods were developed to identify and monitor the residual proteins of TCMIs in industry. Read More

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Nitrogen fertilizer affects rhizosphere Cd re-mobilization by mediating gene AmALM2 and AmALMT7 expression in edible amaranth roots.

J Hazard Mater 2021 Jun 6;418:126310. Epub 2021 Jun 6.

Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China. Electronic address:

In-situ stabilization of Cd-contaminated farmland is a commonly used remediation technology. Yet, rhizosphere metabolites (e.g. Read More

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Proteomic portraits reveal evolutionarily conserved and divergent responses to spinal cord injury.

Mol Cell Proteomics 2021 Jun 12:100096. Epub 2021 Jun 12.

Division of Neurosurgery, University of British Columbia, Vancouver, BC, Canada.

Despite the emergence of promising therapeutic approaches in preclinical studies, the failure of large-scale clinical trials leaves clinicians without effective treatments for acute spinal cord injury (SCI). These trials are hindered by their reliance on detailed neurological examinations to establish outcomes, which inflate the time and resources required for completion. Moreover, therapeutic development takes place in animal models whose relevance to human injury remains unclear. Read More

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Self-Assembled Nanobodies as Selectively Targeted, Nanostructured, and Multivalent Materials.

ACS Appl Mater Interfaces 2021 Jun 15. Epub 2021 Jun 15.

Laboratory for Environmental and Life Sciences, University of Nova Gorica Nova Gorica 5000, Slovenia.

Nanobodies represent valuable tools in advanced therapeutic strategies but their small size (∼2.5 × ∼ 4 nm) and limited valence for interactions might pose restrictions for applications, especially regarding their modest capacity for multivalent and cooperative interaction. In this work, modular protein constructs have been designed, in which nanobodies are fused to protein domains to provide further functionalities and to favor oligomerization into stable self-assembled nanoparticles. Read More

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Mangiferin Promotes Bregs Level, Activates Nrf2 Antioxidant Signaling, and Inhibits Proinflammatory Cytokine Expression in Murine Splenic Mononuclear Cells In Vitro.

Curr Med Sci 2021 Jun 15. Epub 2021 Jun 15.

Department of Cardiovascular Surgery, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.

Recent studies indicated that regulatory B cells (Bregs) and nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant signaling pathway play important roles in the pathogenesis of chronic graft-versus-host disease (cGVHD). Mangiferin (MA), a polyphenol compound, has been reported to activate Nrf2/antioxidant-responsive element (ARE) signaling pathway. This study was aimed to investigate the effects of MA on Bregs and Nrf2 antioxidant signaling in murine splenic mononuclear cells (MNCs) in vitro. Read More

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Selenium deficiency causes apoptosis through endoplasmic reticulum stress in swine small intestine.

Biofactors 2021 Jun 15. Epub 2021 Jun 15.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Selenium (Se) plays a crucial role in intestinal health. However, the specific mechanism by which deficiency of Se causes intestinal damage remains unclear. This study was to explore whether Se deficiency can cause ER stress and induce apoptosis in swine small intestine. Read More

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Implementation of the 2021 molecular ESGO/ESTRO/ESP risk groups in endometrial cancer.

Gynecol Oncol 2021 Jun 11. Epub 2021 Jun 11.

Department of Oncology-Pathology, Karolinska Institutet, Department of Pathology and Cytology, Karolinska University Hospital, Stockholm, Sweden. Electronic address:

Introduction: In 2021, a joint ESGO/ESTRO/ESP committee updated their evidence-based guidelines for endometrial cancer, recommending a new risk grouping incorporating both clinicopathologic and molecular parameters. We applied the new risk grouping and compared the results to those of the prior 2016 clinicopathologic system.

Materials And Methods: We classified molecularly a cohort of 604 women diagnosed with endometrial cancer using immunohistochemistry for TP53 and MMR proteins on a tissue microarray, as well as Sanger sequencing for POLE mutations. Read More

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Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide.

Chem Sci 2019 Dec 3;11(3):826-832. Epub 2019 Dec 3.

Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University Beijing 100084 China

The association of K-Ras4B protein with plasma membrane (PM) is required for its signaling activity. Thus, direct inhibition of K-Ras4B-PM interaction could be a potential anti-Ras therapeutic strategy. However, it remains challenging to modulate such protein-PM interaction. Read More

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December 2019

Vitamin C deficiency inhibits non-alcoholic fatty liver disease progression through impaired de novo lipogenesis.

Am J Pathol 2021 Jun 11. Epub 2021 Jun 11.

Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea; Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea. Electronic address:

Despite the increasing clinical importance of nonalcoholic fatty liver disease (NAFLD), little is known about its underlying pathogenesis or specific treatment. The senescence marker protein 30 (SMP30), which regulates the biosynthesis of vitamin C in many mammals except primates and humans, was recently recognized as a gluconolactonase. However, the precise relation between vitamin C and lipid metabolism in NAFLD is not completely understood. Read More

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TIMEOR: a web-based tool to uncover temporal regulatory mechanisms from multi-omics data.

Nucleic Acids Res 2021 Jun 14. Epub 2021 Jun 14.

Center for Computational and Molecular Biology, Brown University, Providence, RI 02912, USA.

Uncovering how transcription factors regulate their targets at DNA, RNA and protein levels over time is critical to define gene regulatory networks (GRNs) and assign mechanisms in normal and diseased states. RNA-seq is a standard method measuring gene regulation using an established set of analysis stages. However, none of the currently available pipeline methods for interpreting ordered genomic data (in time or space) use time-series models to assign cause and effect relationships within GRNs, are adaptive to diverse experimental designs, or enable user interpretation through a web-based platform. Read More

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Desulfovibrio vulgaris, a potent acetic acid-producing bacterium, attenuates nonalcoholic fatty liver disease in mice.

Gut Microbes 2021 Jan-Dec;13(1):1-20

Functional Metabolomic and Gut Microbiome Laboratory, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

The emerging evidence supports the use of prebiotics like herb-derived polysaccharides for treating nonalcoholic fatty liver disease (NAFLD) by modulating gut microbiome. The present study was initiated on the microbiota-dependent anti-NAFLD effect of polysaccharides (APS) extracted from Bunge in high-fat diet (HFD)-fed mice. However, the exact mechanisms underlying the beneficial effects of APS on NAFLD formation remain poorly understood. Read More

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The complete chloroplast genome and phylogenetic analysis of subsp. (Rupr.) P.S.Green & M.C.Chang from Qinghai Province, China.

Mitochondrial DNA B Resour 2021 Jun 3;6(7):1829-1831. Epub 2021 Jun 3.

Key Laboratory of Restoration Ecology in Cold Region of Qinghai Province, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.

subsp. (Rupr.) P. Read More

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Complete chloroplast genome of by sequencing.

Mitochondrial DNA B Resour 2021 Jun 4;6(7):1826-1828. Epub 2021 Jun 4.

College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, P. R. China.

(Mart.) () is an important herbage species, which could provide high-quality feed for livestock and poultry breeding. This paper is the first to report the chloroplast genomes, which were detected by sequencing. Read More

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Wheat ATIs: Characteristics and Role in Human Disease.

Front Nutr 2021 28;8:667370. Epub 2021 May 28.

Laboratory of Food Chemistry, Wageningen University and Research, Wageningen, Netherlands.

Amylase/trypsin-inhibitors (ATIs) comprise about 2-4% of the total wheat grain proteins and may contribute to natural defense against pests and pathogens. However, they are currently among the most widely studied wheat components because of their proposed role in adverse reactions to wheat consumption in humans. ATIs have long been known to contribute to IgE-mediated allergy (notably Bakers' asthma), but interest has increased since 2012 when they were shown to be able to trigger the innate immune system, with attention focused on their role in coeliac disease which affects about 1% of the population and, more recently, in non-coeliac wheat sensitivity which may affect up to 10% of the population. Read More

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Carbohydrate-binding module -mannosylation alters binding selectivity to cellulose and lignin.

Chem Sci 2020 Aug 19;11(34):9262-9271. Epub 2020 Aug 19.

Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100050 China

Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies. In this study, we examine the impact of -glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases. Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates. Read More

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Effects of Water Extract of (Bge.) Kitag. on Different Breast Cancer Cell Lines.

Evid Based Complement Alternat Med 2021 19;2021:6665949. Epub 2021 May 19.

Department of Applied Chemistry, Chaoyang University of Technology, 168 Jifeng East Road, Taichung, Taiwan.

(Bge.) Kitag. (CP) is an important medicinal herb used in Chinese herbal medicine, with a variety of biological activities including anticancer property. Read More

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Exogenous Gene Expression and Insect Resistance in Dual Bt Toxin × 'Neva' Transgenic Plants.

Front Plant Sci 2021 28;12:660226. Epub 2021 May 28.

Forest Department, Forestry College, Hebei Agricultural University, Baoding, China.

(Bt) insecticidal protein genes are important tools in efforts to develop insect resistance in poplar. In this study, the and Bt toxin genes were simultaneously transformed into the poplar variety × 'Neva' by -mediated transformation to explore the exogenous gene expression and insect resistance, and to examine the effects of Bt toxin on the growth and development of larvae after feeding on the transgenic plant. Integration and expression of the transgenes were determined by molecular analyses and the insect resistance of transgenic lines was evaluated in feeding experiments. Read More

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Research Progress on Phytopathogenic Fungi and Their Role as Biocontrol Agents.

Front Microbiol 2021 28;12:670135. Epub 2021 May 28.

College of Plant Protection, Gansu Agricultural University, Lanzhou, China.

Phytopathogenic fungi decrease crop yield and quality and cause huge losses in agricultural production. To prevent the occurrence of crop diseases and insect pests, farmers have to use many synthetic chemical pesticides. The extensive use of these pesticides has resulted in a series of environmental and ecological problems, such as the increase in resistant weed populations, soil compaction, and water pollution, which seriously affect the sustainable development of agriculture. Read More

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Anti-Rheumatoid Arthritic Effects of Paris Saponin VII in Human Rheumatoid Arthritis Fibroblast-Like Synoviocytes and Adjuvant-Induced Arthritis in Rats.

Front Pharmacol 2021 28;12:683698. Epub 2021 May 28.

Country School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, China.

In the pathogenesis of rheumatoid arthritis (RA), rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) have tumor-like characteristics, mainly manifested by hyperproliferation and resistance to apoptosis and then it will erode the bone and cartilage, eventually leading to joint destruction. Paris saponin VII (PS VII) is an active compound derived from a traditional herbal medicine named Maxim, which has anti-tumor, analgesic, and immunomodulatory effects. However, its anti-RA effect has not yet been reported. Read More

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Do microbial protein elicitors PeaT1 obtained from and PeBL1 from enhance defense response against tomato aphid ()?

Saudi J Biol Sci 2021 Jun 25;28(6):3242-3248. Epub 2021 Feb 25.

Department of Biology, Faculty of Science, Princess Nourah Bint Abdul Rehman University, Saudi Arabia.

Tomato aphid () is a destructive insect pest of tomato responsible for huge losses in the production as well in the vegetable industry. In the present in vitro study two protein elicitors, PeaT1 and PeBL1 were considered to study their efficacies to exhibit defense response against tomato aphid. Three different concentrations of both protein elicitors were applied on the tomato seedlings. Read More

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NUCOME: A comprehensive database of nucleosome organization referenced landscapes in mammalian genomes.

BMC Bioinformatics 2021 Jun 13;22(1):321. Epub 2021 Jun 13.

Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai, 200092, China.

Background: Nucleosome organization is involved in many regulatory activities in various organisms. However, studies integrating nucleosome organization in mammalian genomes are very limited mainly due to the lack of comprehensive data quality control (QC) assessment and uneven data quality of public data sets.

Results: The NUCOME is a database focused on filtering qualified nucleosome organization referenced landscapes covering various cell types in human and mouse based on QC metrics. Read More

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Health and nutrition of loggerhead sea turtles (Caretta caretta) in the southeastern United States.

J Anim Physiol Anim Nutr (Berl) 2021 Jun 13. Epub 2021 Jun 13.

Harbor Branch Oceanographic Institute, Florida Atlantic University, Fort Pierce, Florida, USA.

Loggerhead sea turtles (Caretta caretta) are opportunistic carnivores that feed primarily on benthic invertebrates and fish. Sea turtle rehabilitation requires provision of a species-specific, balanced diet that supplies nutrition similar to that of a wild diet; this can be challenging because free-ranging loggerheads' diets vary depending on their life stage and geographic location, with predominant prey species dictated by local availability. The goal of this study was to better understand the nutritional needs of subadult and adult loggerheads in rehabilitation. Read More

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Rubus chingii var. suavissimus alleviates high-fat diet-induced lipid metabolism disorder via modulation of the PPARs/SREBP pathway in Syrian golden hamsters.

J Nat Med 2021 Jun 12. Epub 2021 Jun 12.

School of Pharmacy, Guangxi University of Chinese Medicine, No. 13, Wuhe Avenue, Qingxiu District, Nanning, 530200, Guangxi, China.

While the underlying mechanism remains unknown, Rubus chingii var. suavissimus (S. K. Read More

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Flooding or drought which one is more offensive on pepper physiology and growth?

Mol Biol Rep 2021 Jun 13. Epub 2021 Jun 13.

Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran.

Both extreme usage of water in agriculture i.e., drought and flooding affect physiological and growth aspects of the plant as well as gene expression undertaken in water absorption. Read More

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Comparative transcriptome analysis reveals a potential mechanism for host nutritional manipulation after parasitization by Leptopilina boulardi.

Comp Biochem Physiol Part D Genomics Proteomics 2021 Jun 7;39:100862. Epub 2021 Jun 7.

Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Zhejiang University, Hangzhou 310058, China. Electronic address:

Parasitoids have been extensively found to manipulate nutrient amounts of their hosts to benefit their own development and survival, but the underlying mechanisms are largely unknown. Leptopilina boulardi (Hymenoptera: Figitidae) is a larval-pupal endoparasitoid wasp of Drosophila melanogaster whose survival relies on the nutrients provided by its Drosophila host. Here, we used RNA-seq to compare the gene expression levels of the host midgut at 24 h and 48 h post L. Read More

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Metabolomic profile combined with transcriptomic analysis reveals the value of UV-C in improving the utilization of waste grape berries.

Food Chem 2021 Jun 4;363:130288. Epub 2021 Jun 4.

College of Enology, Viti-Viniculture Engineering Technology Center of State Forestry and Grassland Administration, Shaanxi Engineering Research Center for Viti-Viniculture, Heyang Viti-Viniculture Station, Northwest A&F University, Yangling 712100, China. Electronic address:

Table grape is a popular fruit worldwide. The quality of the appearance of table grapes directly affects their commercial value. Table grape bunches are usually carefully managed during production. Read More

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Full-length transcriptome sequence analysis of Eimeria necatrix unsporulated oocysts and sporozoites identifies genes involved in cellular invasion.

Vet Parasitol 2021 Jun 2;296:109480. Epub 2021 Jun 2.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, 225009, China. Electronic address:

Eimeria necatrix is one of the most pathogenic chicken coccidia and causes avian coccidiosis, an enteric disease of major economic importance worldwide. Eimeria parasites have complex developmental life cycles, with an exogenous phase in the environment and an endogenous phase in the chicken intestine. Oocysts excreted by chickens rapidly undergo meiosis and cell division to form eight haploid sporozoites (SZ). Read More

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Inhibitory effect of thiourea derivatives on the growth of blue-green algae.

J Environ Manage 2021 Jun 10;294:113008. Epub 2021 Jun 10.

Faculty of Science and Marine Environment (FSSM), Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.

High nutrient loading in aquatic environment has become the main causative of harmful algae blooms (HABs) in water resources particularly pond, lake and river. HABs are mostly dominated by microalgae derived from the group of blue-green algae which are capable of releasing harmful toxins. Therefore, this study aims to investigate the inhibitory effects of thiourea derivatives on the growth of such blue-green algae. Read More

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Enhanced phytoremediation of TNT and cobalt co-contaminated soil by AfSSB transformed plant.

Ecotoxicol Environ Saf 2021 Jun 10;220:112407. Epub 2021 Jun 10.

Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. Electronic address:

2,4,6-trinitrotoluene (TNT) and cobalt (Co) contaminants have posed a severe environmental problem in many countries. Phytoremediation is an environmentally friendly technology for the remediation of these contaminants. However, the toxicity of TNT and cobalt limit the efficacy of phytoremediation application. Read More

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Recent advancements in encapsulation of bioactive compounds as a promising technique for meat preservation.

Meat Sci 2021 May 29;181:108585. Epub 2021 May 29.

Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, São Paulo, Brazil. Electronic address:

Encapsulation is currently considered as one the most valuable methods for preserving aromatic compounds or hiding odors, enhancing their thermal and oxidative stability, and expanding their food applications. Indeed, this current article was aimed to provide an overview regarding the encapsulation of plant bioactive compounds and the spray-drying and extrusion processes with a focused discussion regarding the encountered challenges for meat and meat product preservation. Furthermore, different ranges of carbohydrates as wall materials (carriers) besides the process conditions' effects on the encapsulation effectiveness and the particle size of the encapsulated bioactive compounds have been discussed. Read More

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