1,400,427 results match your criteria protein levels


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J Pharmacol Exp Ther 2022 Jul 2. Epub 2022 Jul 2.

Cardiometabolic Diseases Research, Boehringer Ingelheim Pharmaceuticals, Inc, United States

Diabetic nephropathy is a leading cause of end stage renal disease, characterized by endothelial dysfunction and compromised glomerular permeability barrier. Dysregulation of the ANGPT1/ANGPT2 signaling axis is implicated in disease progression. We recently described the discovery of an IgG antibody, O010, with therapeutic potential to elevate circulating endogenous ANGPT1, a TIE2 agonist. Read More

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PLASMA PROTEIN BIOMARKER PROFILING REVEALS MAJOR DIFFERENCES BETWEEN ACUTE LEUKAEMIA, LYMPHOMA PATIENTS AND CONTROLS.

N Biotechnol 2022 Jun 29. Epub 2022 Jun 29.

Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.

Aiming to accommodate the unmet need for easily accessible biomarkers with a focus on biological differences between haematological diseases, the diagnostic value of plasma proteins in acute leukaemias and lymphomas was investigated. A multiplex proximity extension assay (PEA) was used to analyze 183 proteins in diagnostic plasma samples from 251 acute leukaemia and lymphoma patients and compared with samples from 60 healthy controls. Multivariate modelling using partial least square discriminant analysis revealed highly significant differences between distinct disease subgroups and controls. Read More

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Interleukin-2 inducible T cell kinase (ITK) may participate in the anti-bacterial immune response of Nile tilapia via regulating T-cell activation.

Fish Shellfish Immunol 2022 Jun 29. Epub 2022 Jun 29.

State Key Laboratory of Estuarine and Coastal Research, School of Life Sciences, East China Normal University, Shanghai, 200241, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.

Interleukin-2 inducible T cell kinase (ITK) plays a predominant role in the T-cell receptor (TCR) signaling cascade to ensure valid T-cell activation and function. Nevertheless, whether it regulates T-cell response of early vertebrates remains unknown. Herein, we investigated the involvement of ITK in the lymphocyte-mediated adaptive immune response, and its regulation to T-cell activation in the Nile tilapia Oreochromis niloticus. Read More

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LOW PROTEIN DIET DURING LACTATION PROGRAMS HEPATIC METABOLISM IN ADULT MALE AND FEMALE RATS.

J Nutr Biochem 2022 Jun 29:109096. Epub 2022 Jun 29.

Laboratory of Endocrine Physiology, Biology Institute, State University of Rio de Janeiro, RJ, Brazil. Electronic address:

The liver is an essential regulator of energy metabolism, and its function can be disrupted by nutritional alterations. Since liver development continues during breastfeeding nutritional challenges during this period predispose patients to diseases throughout life. A maternal protein-restricted (PR) diet during lactation promotes reductions in the body weight, adiposity, and plasma glucose and insulin, leptin resistance and an increase in corticosterone and catecholamines in adult male rat offspring. Read More

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Estrogen distinctly regulates transcription and translation of lncRNAs and pseudogenes in breast cancer cells.

Genomics 2022 Jun 29:110421. Epub 2022 Jun 29.

College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates.

Estrogen drives key transcriptional changes in breast cancer and stimulates breast cancer cells' growth with multiple mechanisms to coordinate transcription and translation. In addition to protein-coding transcripts, estrogen can regulate long non-coding RNA (lncRNA) transcripts, plus diverse non-coding RNAs including antisense, enhancer, and intergenic. LncRNA genes comprise the majority of human genes. Read More

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A role for Collagen VII in matrix protein secretion.

Matrix Biol 2022 Jun 29. Epub 2022 Jun 29.

Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, PA; The Joan and Joel Rosenbloom Research Center for Fibrotic Diseases, Thomas Jefferson University, Philadelphia, PA; Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA. Electronic address:

Lack of type VII collagen (C7) disrupts cellular proteostasis yet the mechanism remains undescribed. By studying the relationship between C7 and the extracellular matrix (ECM)-associated proteins thrombospondin-1 (TSP1), type XII collagen (C12) and tissue transglutaminase (TGM2) in primary human dermal fibroblasts from multiple donors with or without the genetic disease recessive dystrophic epidermolysis bullosa (RDEB) (n=31), we demonstrate that secretion of each of these proteins is increased in the presence of C7. In dermal fibroblasts isolated from patients with RDEB, where C7 is absent or defective, association with the COPII outer coat protein SEC31 and ultimately secretion of each of these ECM-associated proteins is reduced and intracellular levels are increased. Read More

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The Double Knockdown of the Mitochondrial Uncoupling Protein Isoforms Reveals Partial Redundant Roles During Arabidopsis thaliana Vegetative and Reproductive Development.

Plant Sci 2022 Jun 29:111365. Epub 2022 Jun 29.

Departamento de Ciências Químicas e Biológicas (Setor Genética), Instituto de Biociências, UNESP, CEP 18618-689, Botucatu, SP, Brasil. Electronic address:

Mitochondrial uncoupling proteins (UCPs) are specialized proteins capable of dissipating the proton electrochemical gradient generated in respiration independent of ATP synthesis. Three UCP coding genes with distinct expression patterns have been identified in Arabidopsis thaliana (namely UCP1, UCP2 and UCP3). Here, we generated T-DNA double-insertion mutants (ucp1 ucp2, ucp1 ucp3 and ucp2 ucp3) to investigate the functionality of the Arabidopsis UCP isoforms. Read More

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Colchicine alleviates inflammation and improves diastolic dysfunction in heart failure rats with preserved ejection fraction.

Eur J Pharmacol 2022 Jun 29:175126. Epub 2022 Jun 29.

Department of Cardiology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, 210008, PR China. Electronic address:

Purpose: Several studies have reported that colchicine attenuates cardiac inflammation and improves cardiac function in myocardial infarction and atrial fibrillation. However, no study has investigated its effect on heart failure with preserved ejection fraction (HFpEF). Hence, this study aimed to assess its efficacy in a high salt diet (HSD)-induced HFpEF rat model. Read More

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Kidney targeting of formoterol containing polymeric nanoparticles improves recovery from ischemia reperfusion-induced acute kidney injury in mice.

Kidney Int 2022 Jun 29. Epub 2022 Jun 29.

The University of Arizona R. Ken Coit College of Pharmacy, Skaggs Pharmaceutical Sciences Center, 1703 E. Mabel St. 85721, Tucson, AZ. USA; The University of Arizona College of Medicine, 501 N Campbell Ave. 85724, Tucson, AZ. USA; The University of Arizona, BIO5 Institute, 1657 E Helen St. 85719, Tucson, AZ. USA; Southern Arizona VA Health Care System, USA. Electronic address:

The β adrenergic receptor agonist, formoterol, is an inducer of mitochondrial biogenesis and restorer of mitochondrial and kidney function in acute and chronic models of kidney injury. Unfortunately, systemic administration of formoterol has the potential for adverse cardiovascular effects, increased heart rate, and decreased blood pressure. To minimize these effects, we developed biodegradable and biocompatible polymeric nanoparticles containing formoterol that target the kidney, thereby decreasing the effective dose, and lessen cardiovascular effects while restoring kidney function after injury. Read More

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Effect of childhood vaccination and antibiotic use on pneumococcal populations and genome-wide associations with disease among children in Nepal: an observational study.

Lancet Microbe 2022 Jul 9;3(7):e503-e511. Epub 2022 May 9.

Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK; NIHR Oxford Biomedical Research Centre, Oxford, UK.

Background: Pneumococcal disease is a leading cause of bacterial pneumonia and invasive bacterial disease among children globally. The reason some strains of pneumococci are more likely to cause disease, and how interventions such as vaccines and antibiotics affect pneumococcal strains is poorly understood. We aimed to identify genetic regions under selective pressure and those associated with disease through the analysis of pneumococcal whole-genome sequences. Read More

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Hormone contraceptive use in young women: Altered mood states, neuroendocrine and inflammatory biomarkers.

Horm Behav 2022 Jun 29;144:105229. Epub 2022 Jun 29.

Department of Neuroscience, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada; University of Ottawa Institute of Mental Health Research, 1145 Carling Avenue, K1Z 7K4 Ottawa, Ontario, Canada.

Oral contraceptives are used by millions of women worldwide, yet there are questions regarding the psychological and biological consequences of these medications. Considering that sex steroid hormones can regulate neuroendocrine and behavioral responses to stress, the current study examined mood and stress symptomatologies, as well as circulating levels of cortisol and inflammatory biomarkers among young women (N = 388), of whom, 47.0 % (n = 182) were using a form of hormonal contraception. Read More

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Metabolomic analysis predicted changes in growth rate in Daphnia magna exposed to acetaminophen.

Aquat Toxicol 2022 Jun 25;249:106233. Epub 2022 Jun 25.

Department of Physical and Environmental Sciences and Environmental NMR Centre, University of Toronto Scarborough, Ontario, Canada. Electronic address:

As urbanization and the global population increases, pollutants associated with municipal wastewater such as pharmaceuticals are becoming more prevalent in aquatic environments. Acetaminophen (paracetamol) is a widely used drug worldwide and one of the most frequently detected pharmaceuticals in freshwater ecosystems. This study investigated the impact of acetaminophen on the metabolite profile of Daphnia magna at two life stages; and used these metabolomic findings to hypothesize a potential impact at a higher organismal level which was subsequently tested experimentally. Read More

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Use of ATR-FTIR spectroscopy for analysis of water deficit tolerance in Physalis peruviana L.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jun 25;280:121551. Epub 2022 Jun 25.

Department of Physics, Dr. Ambedkar Government Arts College, 600039 Chennai, Tamil Nadu, India.

Treatments that allow plants to better tolerate water deficit become essential, such as the application of chemical priming. In addition, it is essential to use analyses capable of measuring these effects at the biomolecular level, complementing the other physiological evaluations. In view of the above, this study aimed to evaluate the use of attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy for analyses of water deficit tolerance in Physalis peruviana plants. Read More

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The dual effect of acetate on microglial TNF-α production.

Clinics (Sao Paulo) 2022 Jun 29;77:100062. Epub 2022 Jun 29.

Department of Immunology, Instituto de Ciências Biomédicas (ICB IV), Universidade de São Paulo, São Paulo, SP, Brazil; Nephrology Division, Universidade Federal de São Paulo, São Paulo, SP, Brazil.

Introduction: Short-Chain Fatty Acids (SCFA) are products of intestinal microbial metabolism that can reach the brain and alter microglia in health and disease contexts. However, data are conflicting on the effect of acetate, the most abundant SCFA in the blood, in these cells.

Objective: The authors aimed to investigate acetate as a modulator of the inflammatory response in microglia stimulated with LPS. Read More

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Carbohydrate-derived bicyclic selenazolines as new dual inhibitors (cholinesterases/OGA) against Alzheimer's disease.

Bioorg Chem 2022 Jun 25;127:105983. Epub 2022 Jun 25.

Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Apartado 1203, E-41071 Sevilla, Spain.

Concerned by the urgent need to explore new approaches for the treatment of Alzheimer's disease, we herein describe the synthesis and evaluation of new multitarget molecules. In particular, we have focused our attention on modulating the activity of cholinesterases (AChE, BuChE) in order to restore the levels of the neurotransmitter acetylcholine, and of O-GlcNAcase (OGA), which is associated with hyperphosphorylation of tau protein, in turn related to the formation of neurofibrillary tangles in the brain. Specifically, we considered the possibility of using carbohydrate-fused 1,3-selenazolines, decorated with a 2-alkylamino or 2-alkoxy moieties. Read More

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Tetrahydrocurcumin improves lipopolysaccharide-induced myocardial dysfunction by inhibiting oxidative stress and inflammation via JNK/ERK signaling pathway regulation.

Phytomedicine 2022 Jun 18;104:154283. Epub 2022 Jun 18.

Department of Cardiovascular Surgery, The First Affiliated Hospital, The Air Force Medical University, Xi'an, Shaanxi 710032, China. Electronic address:

Background: Acute myocardial dysfunction in patients with sepsis is attributed to oxidative stress, inflammation, and cardiomyocyte loss; however, specific drugs for its prevention are still lacking. Tetrahydrocurcumin (THC) has been proven to contribute to the prevention of various cardiovascular diseases by decreasing oxidative stress and inflammation. This study was performed to investigate the functions and mechanism of action of THC in septic cardiomyopathy. Read More

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SOX9 is a target of miR-134-3p and miR-224-3p in breast cancer cell lines.

Mol Cell Biochem 2022 Jul 2. Epub 2022 Jul 2.

GMP & T Cell Therapy Unit, German Cancer Research Center (DKFZ), 210, Im Neuenheimer Feld 280, D-69120, Heidelberg, Germany.

The transcription factor SOX9 represents an important mediator of breast cancer progression. miRNAs are small non-coding RNAs inhibiting translation of target genes upon interaction with the 3'-UTR region of respective mRNA molecules. Deregulated miRNA expression is involved in hallmarks of cancer like sustained proliferation and inhibition of apoptosis. Read More

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Modulation of endoplasmic reticulum stress via sulforaphane-mediated AMPK upregulation against nonalcoholic fatty liver disease in rats.

Cell Stress Chaperones 2022 Jul 2. Epub 2022 Jul 2.

Health Radiation Research, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Nonalcoholic fatty liver disease (NAFLD) is a major health concern. Endoplasmic reticulum (ER) stress, inflammation, and metabolic dysfunctions may be targeted to prevent the progress of nonalcoholic fatty liver disease. Sulforaphane (SFN), a sulfur-containing compound that is abundant in broccoli florets, seeds, and sprouts, has been reported to have beneficial effects on attenuating metabolic diseases. Read More

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OsPP65 Negatively Regulates Osmotic and Salt Stress Responses Through Regulating Phytohormone and Raffinose Family Oligosaccharide Metabolic Pathways in Rice.

Rice (N Y) 2022 Jul 2;15(1):34. Epub 2022 Jul 2.

Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.

Although type 2C protein phosphatases (PP2Cs) have been demonstrated to play important roles in regulating plant development and various stress responses, their specific roles in rice abiotic stress tolerance are still largely unknown. In this study, the functions of OsPP65 in rice osmotic and salt stress tolerance were investigated. Here, we report that OsPP65 is responsive to multiple stresses and is remarkably induced by osmotic and salt stress treatments. Read More

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Green tea EGCG effectively alleviates experimental colitis in middle-aged male mice by attenuating multiple aspects of oxi-inflammatory stress and cell cycle deregulation.

Biogerontology 2022 Jul 2. Epub 2022 Jul 2.

Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, India.

Age-dependent increased risk of inflammatory bowel diseases such as ulcerative colitis is being increasingly realized, and yet therapies targeting this disorder within the purview of aging are limited. The present study attempted to assess the efficacy of green tea epigallocatechin gallate (EGCG) consumption in preventing the severity and progression of dextran sulphate sodium (DSS)-induced ulcerative colitis in 18 months old middle-aged male mice. Acute colitis was induced in animals using DSS and protective effects of EGCG consumption were examined. Read More

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A method for the analysis of methylmercury and total Hg in fungal matrices.

Appl Microbiol Biotechnol 2022 Jul 2. Epub 2022 Jul 2.

Department of Analytical Chemistry, Gdańsk University of Technology, 11/12 G. Narutowicza Street, 80-233, Gdańsk, Poland.

The aim of the study was to develop an efficient method for the determination of monomethyl-mercury (MeHg) and total mercury (THg) content in materials such as fungal sporocarps and sclerotia. Certified Reference Materials (CRMs) with the assigned values of MeHg and THg as well as the control materials (dried mushrooms) with known content of THg were evaluated for method validation. Recovery of MeHg from reference materials was at the following levels: from tuna fish at 87. Read More

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An inducible potato (E,E)-farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco.

Plant J 2022 Jul 2. Epub 2022 Jul 2.

Molecular Plant Biology and Biotechnology Lab, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru - 560065, India.

Terpene synthases (TPSs) have diverse biological functions in plants. Though the roles of TPSs in herbivore defense are well established in many plant species, their role in bacterial defense has been scarce and is emerging. Through functional genomics, here we report the in planta role of potato (Solanum tuberosum) terpene synthase (StTPS18) in bacterial defense. Read More

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Proteomics-based investigation of cerebrovascular molecular mechanisms in cerebral amyloid angiopathy by the FFPE-LMD-PCT-SWATH method.

Fluids Barriers CNS 2022 Jul 1;19(1):56. Epub 2022 Jul 1.

Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.

Background: Cerebral amyloid angiopathy (CAA) occurs in 80% of patients with Alzheimer's disease (AD) and is mainly caused by the abnormal deposition of Aβ in the walls of cerebral blood vessels. Cerebrovascular molecular mechanisms in CAA were investigated by using comprehensive and accurate quantitative proteomics.

Methods: Concerning the molecular mechanisms specific to CAA, formalin-fixed paraffin-embedded (FFPE) sections were prepared from patients having AD neuropathologic change (ADNC) with severe cortical Aβ vascular deposition (ADNC +/CAA +), and from patients having ADNC without vascular deposition of Aβ (ADNC +/CAA -; so called, AD). Read More

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Alterations in circulating levels of vitamin D binding protein, total and bioavailability of vitamin D in diabetic retinopathy patients.

BMC Endocr Disord 2022 Jul 1;22(1):169. Epub 2022 Jul 1.

Tehran University of Medical Sciences, Tehran, Iran.

Aims: This study aimed to investigate the association between circulating levels of vitamin D binding protein (VDBP) and its genotypes and diabetic retinopathy risk.

Methods: This case-control study recruited 154 patients with type 2 diabetes mellitus; 62 with diabetic retinopathy (DR) and 92 without DR and diabetic nephropathy (DN). Circulating levels of 25-hydroxyvitamin D3 and VDBP levels were measured in the patients. Read More

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Luteolin alleviates inflammation and autophagy of hippocampus induced by cerebral ischemia/reperfusion by activating PPAR gamma in rats.

BMC Complement Med Ther 2022 Jul 1;22(1):176. Epub 2022 Jul 1.

Department of Neurology, Yantaishan Hospital, No.10087, Keji Avenue, Laishan District, Yantai, 264000, China.

Background: Luteolin, a flavonoid compound with anti-inflammatory activity, has been reported to alleviate cerebral ischemia/reperfusion (I/R) injury. However, its potential mechanism remains unclear.

Methods: The binding activity of luteolin to peroxisome proliferator-activated receptor gamma (PPARγ) was calculated via molecular docking analysis. Read More

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Bexarotene-induced cell death in ovarian cancer cells through Caspase-4-gasdermin E mediated pyroptosis.

Sci Rep 2022 Jul 1;12(1):11123. Epub 2022 Jul 1.

Department of Reproductive Medicine, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba, 260-8670, Japan.

Bexarotene selectively activates retinoid X receptor, which is a commonly used anticancer agent for cutaneous T-cell lymphoma. In this study, we aimed to investigate the anticancer effect of bexarotene and its underlying mechanism in ovarian cancer in vitro. The ES2 and NIH:OVACAR3 ovarian cancer cell lines were treated with 0, 5, 10, or 20 µM of bexarotene. Read More

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Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis.

Acta Neuropathol 2022 Jul 1. Epub 2022 Jul 1.

Department of Stem Cell Biology, University Hospital Erlangen, Friedrich-Alexander University of Erlangen-Nürnberg (FAU), Erlangen, Germany.

Amyotrophic lateral sclerosis (ALS) is a fatal disease characterized by aberrant alternative splicing (AS). Nuclear loss and cytoplasmic accumulation of the splicing factor TDP-43 in motor neurons (MN) are hallmarks of ALS at late stages of the disease. However, it is unknown if altered AS is present before TDP-43 pathology occurs. Read More

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Amino acid sensor GCN2 promotes SARS-CoV-2 receptor ACE2 expression in response to amino acid deprivation.

Commun Biol 2022 Jul 1;5(1):651. Epub 2022 Jul 1.

Zhongshan Hospital, State Key Laboratory of Medical Neurobiology, Institute for Translational Brain Research, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, 200032, China.

Angiotensin-converting enzyme 2 (ACE2) has been identified as a primary receptor for severe acute respiratory syndrome coronaviruses 2 (SARS-CoV-2). Here, we investigated the expression regulation of ACE2 in enterocytes under amino acid deprivation conditions. In this study, we found that ACE2 expression was upregulated upon all or single essential amino acid deprivation in human colonic epithelial CCD841 cells. Read More

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PARP1 and XRCC1 exhibit a reciprocal relationship in genotoxic stress response.

Cell Biol Toxicol 2022 Jul 1. Epub 2022 Jul 1.

Molecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Constance, Germany.

PARP1 (aka ARTD1) acts as a prime sensor of cellular genotoxic stress response. PARP1 detects DNA strand breaks and subsequently catalyzes the formation of poly(ADP-ribose) (PAR), which leads to the recruitment of the scaffold protein XRCC1 during base excision and single strand break repair and the assembly of multi-protein complexes to promote DNA repair. Here, we reveal that the recruitment of either protein to sites of DNA damage is impeded in the absence of the other, indicating a strong reciprocal relationship between the two DNA repair factors during genotoxic stress response. Read More

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Effect of modified Total Body Recumbent Stepper training on exercise capacity and thioredoxin in COPD: a randomized clinical trial.

Sci Rep 2022 Jul 1;12(1):11139. Epub 2022 Jul 1.

Department of Respiratory Diseases, Medical School, Hunan University of Chinese Medicine/Key Laboratory of Vascular Biology and Translational Medicine in Hunan Province, Changsha, 410208, China.

Exercise intolerance is one of the major symptoms of chronic obstructive pulmonary disease (COPD). Exercise training can benefit COPD patients, but the underlying mechanism remains unclear. The modified Total Body Recumbent Stepper (TBRS, Nustep-T4) can benefit patients with stroke, spinal cord injury and amyotrophic lateral sclerosis. Read More

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