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Characteristics and outcomes of cancer patients with covid-19 at a safety-net hospital.

Cancer Treat Res Commun 2021 Jun 10;28:100418. Epub 2021 Jun 10.

Department of Hematology and Oncology, Capital Health Regional Medical Center, Trenton, NJ, United States.

Patients with cancer are a vulnerable population during the COVID-19 pandemic due to underlying immunosuppression, pre-existing comorbidities, and poor nutrition. There is a lack of data describing the disease course of cancer patients with COVID-19 disease. Therefore, we analyzed data from cancer patients with COVID-19 who were admitted to our hospital. Read More

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MicroRNA-383-5p regulates osteogenic differentiation of human periodontal ligament stem cells by targeting histone deacetylase 9.

Authors:
Lan Ma Di Wu

Arch Oral Biol 2021 May 25;129:105166. Epub 2021 May 25.

Department of Stomatology, Jingmen No.1 People's Hospital, China. Electronic address:

Objective: Human periodontal ligament stem cells (hPDLSCs) play an important role in regenerative engineering technology for periodontal therapy. The mechanism of microRNA (miR)-383-5p in osteogenic differentiation needs further exploration. This study aimed at investigating the potential role of miR-383-5p in the osteogenic differentiation of hPDLSCs. Read More

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Epidemiological feature, viral shedding, and antibody seroconversion among asymptomatic SARS-CoV-2 carriers and symptomatic/presymptomatic COVID-19 patients.

J Infect Public Health 2021 May 27;14(7):845-851. Epub 2021 May 27.

Department of Epidemiology, Second Military Medical University, Shanghai 200433, China. Electronic address:

Background: Novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pandemic. However, data concerning the epidemiological features, viral shedding, and antibody dynamics between asymptomatic SARS-CoV-2 carriers and COVID-19 patients remain controversial.

Methods: We enrolled 193 SARS-CoV-2 infected subjects in Ningbo and Zhoushan, Zhejiang, China, from January 21 to March 6, 2020. Read More

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HOXB5 promotes the progression of breast cancer through wnt/beta-catenin pathway.

Pathol Res Pract 2020 Jul 13;224:153117. Epub 2020 Jul 13.

National Clinical Research Center for Normal Aging and Geriatric & The Key Lab of Normal Aging and Geriatric, Institute of Geriatric, PLA General Hospital, Beijing, China. Electronic address:

Objective: The present study was designed to explore the function of HOXB5 in breast cancer and related signaling pathway.

Methods: Breast cancer tissues and non-cancerous tissues were collected from 82 cases who were pathologically diagnosed with breast cancer. The mRNA level of HOXB5 was detected via quantitative real-time polymerase chain reaction (qRT-PCR). Read More

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High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.

Transl Oncol 2021 Jun 9;14(8):101146. Epub 2021 Jun 9.

Department of Otolaryngology Head & Neck Surgery, The Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, No. 639, Zhi-Zao-Ju Road, Shanghai 200011, China; Ear Institute, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China. Electronic address:

Head and neck paragangliomas (HNPGLs) are rare neoplasms that represent difficult treatment paradigms in neurotology. Germline mutations in genes encoding succinate dehydrogenase (SDH) are the cause of nearly all familial HNPGLs. However, the molecular mechanisms underlying tumorigenesis remain unclear. Read More

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In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus Carica L.).

Plant Physiol Biochem 2021 Jun 1;166:191-202. Epub 2021 Jun 1.

Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, P.O. Box 65186-71441, Iran. Electronic address:

Fig tree (Ficus carica L.) is a precious fruit tree in semi-arid and arid areas worldwide which has difficulties in its conventional breeding programs. This study was carried out to make new genotypes with superior features based on the ploidy induction method. Read More

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Orally administered nano-polystyrene caused vitellogenin alteration and oxidative stress in the red swamp crayfish (Procambarus clarkii).

Sci Total Environ 2021 May 29;791:147984. Epub 2021 May 29.

Dept. Life Sciences, University of Trieste, via L. Giorgieri 5, 34127 Trieste, Italy. Electronic address:

Nanoplastics (≤100 nm) represent the smallest fraction of plastic litter and may result in the aquatic environment as degradation products of larger plastic material. To date, few studies focused on the interactions of micro- and nanoplastics with freshwater Decapoda. The red swamp crayfish (Procambarus clarkii, Girard, 1852) is an invasive species able to tolerate highly perturbed environments. Read More

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The effect of Raphanus sativus L. seeds on regulation of intestinal motility in rats consuming a high-calorie diet.

Biomed Pharmacother 2021 Jun 9;140:111705. Epub 2021 Jun 9.

School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing, PR China. Electronic address:

Purpose: The purpose of this study was to explore the effects of a short-term high-calorie diet and the regulation mechanism of Raphanus sativus L. seeds (RSL seeds) on the intestinal motility of young rats.

Methods: We fed 20 Specific Pathogen Free (SPF) 4-week-old male Sprague-Dawley (SD) rats special high-calorie diet for 3 days and then randomized them to a high-calorie diet group (HCG, 10 rats) and an RSL seeds treatment group (TG, 10 rats). Read More

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Level of Maternal Respiratory Syncytial Virus (RSV) F Antibodies in Hospitalized Children and Correlates of Protection.

Int J Infect Dis 2021 Jun 9. Epub 2021 Jun 9.

Biomedical Research Center, Qatar University, Qatar; College of Health Sciences, Qatar University, Qatar. Electronic address:

Background: RSV is a major cause of lower respiratory infections among children, where no vaccine is available. The stabilized form of the fusion (F) protein, pre-F, is a leading vaccine candidate targeting different populations, including pregnant women. This study aimed to determine the magnitude and nature of RSV-directed maternal antibodies (matAbs) in hospitalized children with RSV infection. Read More

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mA modification of RNA and its role in cancer, with a special focus on lung cancer.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

ICAR-Indian Veterinary Research Institute, Izatnagar, UP, India.

Epitranscriptomics involves functionally relevant biochemical modifications of RNA taking place at the transcriptome level without a change in the sequence of ribonucleotides. Several types of modifications that affect the processing and function of differentRNA types have been reported. Methylation at N of Adenosine called mA is one such modification, quite widespread in occurrence and reported in snRNAs, lncRNAs, circRNAs, rRNAs, miRNAs, and most abundantly, in mRNAs. Read More

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Revealing the Mechanism of "Huai Hua San" in the Treatment of Ulcerative Colitis based on Network Pharmacology and Experimental Study.

J Ethnopharmacol 2021 Jun 9:114321. Epub 2021 Jun 9.

Faculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China; Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Wuhan, 430065, China; Collaborative Innovation Center of Traditional Chinese Medicine of New Products for Geriatrics Hubei Province, Wuhan, 430065, China. Electronic address:

Ethnopharmacological Relevance: "Huai Hua San" (HHS) is one of the first hundred ancient classic prescriptions drugs, which is commonly used to treat hemorrhoids, colitis, and other symptoms of wind heat in stool. However, the potential molecular mechanism of action of this substance remains unclear.

Aims Of The Study: In this study, we explored the active compounds of HHS for the treatment of ulcerative colitis (UC), predicted the potential targets of the drug, and studied its mechanism of action through network pharmacology via in vitro and in vivo experiments. Read More

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Arsenic disrupts neuronal insulin signaling through increasing free PI3K-p85 and decreasing PI3K activity.

Toxicol Lett 2021 Jun 9. Epub 2021 Jun 9.

Laboratory of Pharmacology, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Rd, Bangkok, 10210, Thailand; Environmental Toxicology Program, Chulabhorn Graduate Institute, 54 Kamphaeng Phet 6 Rd, Bangkok, 10210, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), Ministry of Higher Education, Science, Research and Innovation, Thailand.

Previously, we reported that prolonged arsenic exposure impaired neuronal insulin signaling. Here we have further identified novel molecular mechanisms underlying neuronal insulin signaling impairment by arsenic. Arsenic treatment altered insulin dose-response curve and reduced maximum insulin response in differentiated human neuroblastoma SH-SY5Y cells, suggesting that arsenic hindered neuronal insulin signaling in a non-competitive like manner. Read More

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Beneficial effect of the methanolic leaf extract of Allium hookeri on stimulating glutathione biosynthesis and preventing impaired glucose metabolism in type 2 diabetes.

Arch Biochem Biophys 2021 Jun 9:108961. Epub 2021 Jun 9.

Chemical Biology Lab, Institute of Advanced Study in Science and Technology (IASST) Paschim Boragaon, Guwahati, 35, India; Assam Down Town University, Panikhaiti, Assam, 781068, India. Electronic address:

Oxidative stress resulting from the depletion of glutathione (GSH) level plays a vital role in generating various degenerative diseases, including type 2 diabetes (T2D). We tested the hypothesis that depleted glutathione levels can be enhanced and the impaired glucose metabolism can be prevented by supplementing Allium hookeri, a herb rich in organosulfur compounds, in a High Fat (HF) diet-induced T2D Male Sprague Dawley rat model. The experimental rats were divided into three groups (n = 6), namely normal diet, high-fat diet, and high-fat diet treated with A. Read More

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Aortic Angiosarcoma in Association with Endovascular Aneurysm Repair: Case Report and Review of the Literature.

Am J Case Rep 2021 Jun 12;22:e931740. Epub 2021 Jun 12.

Division of Clinical Pathology, Niigata University Faculty of Medicine, Niigata, Japan.

BACKGROUND Primary aortic sarcoma often poses diagnostic challenges for pathologists and clinicians because of a very low incidence and controversy over nomenclature and definition. We report a case of aortic angiosarcoma in association with a graft. We also conducted a clinicopathological review of cases of primary aortic sarcomas associated with implanted grafts. Read More

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FGF21 promotes wound healing of rat brain microvascular endothelial cells through facilitating TNF-α-mediated VEGFA and ERK1/2 signaling pathway.

Adv Clin Exp Med 2021 Jun 11. Epub 2021 Jun 11.

No. 1 Department of Orthopedics, The Third People's Hospital, Cixi, China.

Background: Wound healing is an essential physiological process in recovery after microsurgery.

Objectives: To further understand the functions of fibroblast growth factor 21 (FGF21), the roles of this factor were examined and its correlations with inflammation, vascular endothelial growth factor A (VEGFA) and ERK1/2 signaling pathway activation were analyzed.

Material And Methods: Rat brain microvascular endothelial cells (RBMECs) were treated with interleukin (IL)-1β and used for the experiments. Read More

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IL-10 and TGF-β cytokines decrease immune activation during normothermic ex vivo machine perfusion of the rat liver.

Liver Transpl 2021 Jun 12. Epub 2021 Jun 12.

Department of Surgery, Division of Transplantation, University of Wisconsin, School of Medicine and Public Health, Madison.

Background: Normothermic ex vivo liver perfusion (NEVLP) is a novel system for organ preservation which may improve over static cold storage (CS) clinically and offers the chance for graft modification prior to transplantation. Although recent studies have shown the presence of inflammatory molecules during perfusion, none have yet shown the effects of NEVLP on liver-resident immune cell activation. We investigated the effects of NEVLP on liver-resident immune cell activation and assessed the ability of anti-inflammatory cytokines IL-10 and TGF-β to improve organ function and reduce immune activation during perfusion. Read More

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Effects of Apigenin on the Expression of LOX-1, Bcl-2, and Bax in Hyperlipidemia Rats.

Chem Biodivers 2021 Jun 12. Epub 2021 Jun 12.

Chengde Medical College: Chengde Medical University, Biochemistry, Anyuan Road, Chengde, CHINA.

We aimed to investigate the impact of apigenin on LOX-1, Bcl-2, and Bax expression in hyperlipidemia rats and explore the possible molecular pathological mechanism of apigenin in improving hyperlipidemia and preventing atherosclerosis. In hyperlipidemia models, the levels of TC, TG, LDL and the LOX-1 protein expression were apparently increased ( P <0.01), while the HDL levels and the ratio of Bcl-2/Bax were reduced significantly ( P <0. Read More

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Intrinsically Disordered Osteopontin Fragment Orders During Interfacial Calcium Oxalate Mineralization.

Angew Chem Int Ed Engl 2021 Jun 12. Epub 2021 Jun 12.

Max Planck Institute for Polymer Research: Max-Planck-Institut fur Polymerforschung, molecular spectroscopy, GERMANY.

Calcium oxalate (CaC 2 O 4 ) is the major component of kidney stone, a prevalent chronic human disease worldwide. The acidic osteopontin (OPN) protein in human urine can effectively inhibit the growth of CaC 2 O 4 crystals, thereby acting as a potent stone preventer. Previous studies in bulk solution all attest to the importance of binding and recognition of OPN at the CaC 2 O 4 mineral surface, yet molecular level insights into the active interface during CaC 2 O 4 mineralization are still lacking. Read More

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Bumblebee resilience to climate change, through plastic and adaptive responses.

Glob Chang Biol 2021 Jun 12. Epub 2021 Jun 12.

Laboratory of Agrozoology, Department Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium.

Bumblebees are ubiquitous, cold-adapted eusocial bees found worldwide from subarctic to tropical regions of the world. They are key pollinators in most temperate and boreal ecosystems, and both wild and managed populations are significant contributors to agricultural pollination services. Despite their broad ecological niche at the genus level, bumblebee species are threatened by climate change, particularly by rising average temperatures, intensifying seasonality and the increasing frequency of extreme weather events. Read More

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A hierarchical deep learning based approach for multi-functional enzyme classification.

Protein Sci 2021 Jun 12. Epub 2021 Jun 12.

Computational Biology Research Lab, National University of Computer and Emerging Sciences, Islamabad, Pakistan.

Enzymes are critical proteins in every organism. They speed up essential chemical reactions, help fight diseases and have a wide use in the pharmaceutical and manufacturing industries. Wet lab experiments to figure out an enzyme's function are time consuming and expensive. Read More

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P38α-MAPK phosphorylates Snapin and reduces Snapin-mediated BACE1 transportation in APP-transgenic mice.

FASEB J 2021 Jul;35(7):e21691

Department of Neurology, Saarland University, Homburg/Saar, Germany.

Amyloid β peptide (Aβ) is the major pathogenic molecule in Alzheimer's disease (AD). BACE1 enzyme is essential for the generation of Aβ. Deficiency of p38α-MAPK in neurons increases lysosomal degradation of BACE1 and decreases Aβ deposition in the brain of APP-transgenic mice. Read More

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mTRIP, an Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution.

Methods Mol Biol 2021 ;2275:247-263

Institut Pasteur, Stem Cells and Development, Department of Developmental and Stem Cell Biology, CNRS UMR3738 Team "Stability of Nuclear and Mitochondrial DNA", Paris, France.

Mitochondrial physiology and metabolism are closely linked to replication and transcription of mitochondrial DNA (mtDNA). However, the characterization of mtDNA processing is poorly defined at the single-cell level. We developed mTRIP (mitochondrial Transcription and Replication Imaging Protocol), an imaging approach based on modified fluorescence in situ hybridization (FISH), which simultaneously reveals mitochondrial structures committed to mtDNA initiation of replication as well as the mitochondrial RNA (mtRNA) content at the single-cell level in human cells. Read More

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January 2021

Liposomal Delivery of Cyclocreatine Impairs Cancer Cell Bioenergetics Mediating Apoptosis.

Methods Mol Biol 2021 ;2275:173-186

Department of Pharmaceutical Sciences, College of Pharmacy-Glendale Campus, Midwestern University, Glendale, AZ, USA.

Creatine kinase (CK) enzyme overexpression has been suggested to play a role in the process of tumorigenesis and metastasis. Cyclocreatine (CCR) is a substrate analog of creatine kinase (CK), where its phosphorylated form is a poor phosphate donor in comparison with native bioenergetic molecule, creatine phosphate (Cr-P). The compound CCR has been shown to markedly inhibit the growth of a broad spectrum of cancers, both in vitro and in vivo. Read More

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January 2021

Serum cartilage oligomeric matrix protein is correlated with quantitative magnetic resonance imaging and arthroscopic cartilage findings in anterior cruciate ligament deficient knees without osteoarthritic changes.

Clin Rheumatol 2021 Jun 12. Epub 2021 Jun 12.

Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka, 545-8585, Japan.

Introduction/objectives: To investigate the association between serum biomarker [cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-3 (MMP-3)] levels and clinical, magnetic resonance imaging (MRI), and arthroscopic findings in anterior cruciate ligament (ACL)-deficient knees without osteoarthritic changes on radiographs.

Method: Patients with ACL injury of Kellgren-Lawrence grade 0 or 1 were enrolled. Serum COMP and MMP-3 levels were measured preoperatively. Read More

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Identification of key genes in the tumor microenvironment of lung adenocarcinoma.

Med Oncol 2021 Jun 12;38(7):83. Epub 2021 Jun 12.

Key Laboratory of Medical Imaging and Artifical Intelligence of Hunan Province, 423000, Chenzhou, P. R. China.

The tumor microenvironment plays an important role in tumor development and progression, but the role of immune and stromal cells in this environment has not been sufficiently studied. In this study, we aimed to identify key genes associated with the microenvironment of lung adenocarcinoma (LUAD). Raw data for stromal and immune cells in malignant tumors were downloaded from The Cancer Genome Atlas (TCGA). Read More

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Anticancer activity of Helicobacter pylori ribosomal protein (HPRP) with iRGD in treatment of colon cancer.

J Cancer Res Clin Oncol 2021 Jun 12. Epub 2021 Jun 12.

Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Purpose: As the conventional therapeutic approaches were not completely successful in the treatment of colon cancer, there is still a need for finding the most efficient therapeutic agents. Here we investigated the anticancer activity of HPRP-A1 that was derived from the N-terminal region of Helicobacter pylori ribosomal protein L1 (RpL1) alone or in combination with tumor-homing peptide iRGD and 5-Fluorouracil (5FU) on colon cancer cell lines (CT26 and HT29) and isograft models of colon cancer.

Method: We assessed the tumor growth inhibitory activity of HPRP-A1 with or without iRGD and 5FU on colon cancer in vitro and in vivo. Read More

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Protective effect of transient receptor potential melastatin 2 inhibitor A10 on oxygen glucose deprivation/reperfusion model.

Zhejiang Da Xue Xue Bao Yi Xue Ban 2021 Feb;50(1):106-112

Department of Human Anatomy,Histology and Embryology,School of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China.

:To investigate the effect of transient receptor potential melastatin 2 (TRPM2) inhibitor A10 on oxygen glucose deprivation/reperfusion (OGD/R) injury in SH-SY5Y cells.:Human neuroblastoma SH-SY5Y cells were subject to OGD/R injury,and then were divided into blank control group,model control group and A10 group randomly. The cell survival rate was detected by cell counting kit 8 (CCK-8); the level of cellular reactive oxygen species (ROS) was detected by reactive oxygen detection kit; the mitochondrial membrane potential was detected by tetramethylrhodamine (TMRM) method; the number of apoptotic cells was detected by TUNEL apoptosis assay kit; the protein expression level of cleaved caspase 3 was detected by Western blot.:Compared with 3,20,30,50, has lower cytotoxicity and better inhibition effect on channel activity. Compared with the model control group,ROS level was reduced,the mitochondrial membrane potential was improved,the number of apoptosis cells was reduced ,and the expression of cleaved caspase 3 was significantly reduced in the A10 group(all <0. Read More

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February 2021

Edible Materials in Tissue Regeneration.

Macromol Biosci 2021 Jun 12:e2100114. Epub 2021 Jun 12.

School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, 518057, China.

Edible materials have attracted increasing attention because of their excellent properties including availability, biocompatibility, biological activity, and biodegradability. Natural polysaccharides, phenolic compounds, and proteins are widely used in tissue regeneration. To better characterize their healing effect, this review article describes the applications of edible materials in tissue regeneration including wound healing and bone tissue regeneration. Read More

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Naringenin prevents pregnancy-induced hypertension via suppression of JAK/STAT3 signaling pathway in mice.

Int J Clin Pract 2021 Jun 12:e14509. Epub 2021 Jun 12.

Department of Obstetrics, ZiBo Central Hospital, No.54 of Gongqingtuan West Road, Zhangdian District, Zibo, 255000, Shandong, China.

Background: Pregnancy-induced hypertension (PIH) is characterized by high blood pressure during pregnancy, which causes perinatal and maternal mortality. Inflammation, oxidative stress and the JAK2/STAT3 signaling pathway have been reported to play critical roles in the pathogenies of PIH. Due to the safety and side effects of current treatments for PIH, searching for new therapeutic agents is urgently needed. Read More

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VE-cadherin N-glycosylation modified by GnT-V regulates VE-cadherin/β-catenin interaction and monocyte adhesion.

Exp Physiol 2021 Jun 12. Epub 2021 Jun 12.

College of Pharmacy, Chongqing Medical University, Chongqing, 400016, P.R. China.

New Findings: What is the central question of this study? Inflammation-induced monocyte adhesion is the initiator of most vascular diseases. The underlying mechanisms mediating monocyte adhesion remain to be fully clarified. What is the main finding and its importance? GnT-V medicated N-glycosylation on VE-cadherin regulates the dissociation of VE-cadherin/β-catenin complex to modulate the monocyte adhesion, but GnT-V overexpression can not rescue monocyte adhesion induced by IL-1β. Read More

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