15,758 results match your criteria proposed protocol


Technical framework for wastewater-based epidemiology of SARS-CoV-2.

Sci Total Environ 2021 Jun 11;791:148271. Epub 2021 Jun 11.

Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China. Electronic address:

Wastewater-based epidemiology (WBE) is expected to become a powerful tool to monitor the dissemination of SARS-CoV-2 at the community level, which has attracted the attention of scholars all over the world. However, there is not yet a standard protocol to guide its implementation. In this paper, we proposed a comprehensive technical and theoretical framework of relative quantification via qPCR for determining the virus abundance in wastewater and estimating the infection ratio in corresponding communities, which is expected to achieve horizontal and vertical comparability of the data using a human-specific biomarker as the internal reference. Read More

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Efficient and effective single-step screening of individual samples for SARS-CoV-2 RNA using multi-dimensional pooling and Bayesian inference.

J R Soc Interface 2021 Jun 16;18(179):20210155. Epub 2021 Jun 16.

Department of Chemistry, University of Utah, Salt Lake City, UT, USA.

Rapid and widespread implementation of infectious disease surveillance is a critical component in the response to novel health threats. Molecular assays are the preferred method to detect a broad range of viral pathogens with high sensitivity and specificity. The implementation of molecular assay testing in a rapidly evolving public health emergency, such as the ongoing COVID-19 pandemic, can be hindered by resource availability or technical constraints. Read More

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Pharmacological and non-pharmacological interventions for osteoporosis: A protocol for an overview with an evidence map and a network meta-analysis of trials.

Medicine (Baltimore) 2021 Jun;100(24):e26429

Clinical College of Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou, China.

Background: Osteoporosis is a common bone disease that has a significant social and economic effect. Many meta-analyses of pharmacological and non-pharmacological treatments for osteoporosis have been reported, but the findings may be contradictory, and both the reporting and methodological quality remain unknown. As a result, an overview that includes a network meta-analysis was proposed to address these issues. Read More

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Entanglement bounds on the performance of quantum computing architectures.

Phys Rev Res 2020 ;2(3)

Joint Center for Quantum Information and Computer Science, NIST/University of Maryland, College Park, Maryland 20742, USA.

There are many possible architectures of qubit connectivity that designers of future quantum computers will need to choose between. However, the process of evaluating a particular connectivity graph's performance as a quantum architecture can be difficult. In this paper, we show that a quantity known as the isoperimetric number establishes a lower bound on the time required to create highly entangled states. Read More

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January 2020

Visible-light-induced intramolecular charge transfer in the radical spirocyclisation of indole-tethered ynones.

Chem Sci 2019 Dec 13;11(5):1353-1360. Epub 2019 Dec 13.

Department of Chemistry, University of York York YO10 5DD UK

Indole-tethered ynones form an intramolecular electron donor-acceptor complex that can undergo visible-light-induced charge transfer to promote thiyl radical generation from thiols. This initiates a novel radical chain sequence, based on dearomatising spirocyclisation with concomitant C-S bond formation. Sulfur-containing spirocycles are formed in high yields using this simple and mild synthetic protocol, in which neither transition metal catalysts nor photocatalysts are required. Read More

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December 2019

Chiral ,'-dioxide/Mg(OTf) complex-catalyzed asymmetric [2,3]-rearrangement of generated ammonium salts.

Chem Sci 2020 Feb 19;11(11):3068-3073. Epub 2020 Feb 19.

Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University Chengdu 610064 China

Catalytic enantioselective [2,3]-rearrangements of generated ammonium ylides from glycine pyrazoleamides and allyl bromides were achieved by employing a chiral ,'-dioxide/Mg complex as the catalyst. This protocol provided a facile and efficient synthesis route to a series of -α-amino acid derivatives in good yields with high stereoselectivities. Moreover, a possible catalytic cycle was proposed to illustrate the reaction process and the origin of stereoselectivity. Read More

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February 2020

Effect of root canal irrigant (sodium hypochlorite & saline) delivery at different temperatures and durations on pre-load and cyclic-loading surface-strain of anatomically different premolars.

J Mech Behav Biomed Mater 2021 Jun 9;121:104640. Epub 2021 Jun 9.

Unit of Endodontology, Division of Restorative Dental Science, UK.

Aim: To evaluate the effect of NaOCl (5%) and saline (control) irrigant delivery at different temperatures and durations on pre-load and cyclic-loading tooth-surface-strain (TSS) on anatomically different premolars.

Methodology: Single-rooted premolars (n = 36), root-canal-prepared in standard manner, were randomly allocated to six irrigation groups: (A1) NaOCl-21 °C; (A2) NaOCl-60 °C; (A3) saline-21 °C then NaOCl-21 °C; (A4) saline-60 °C then NaOCl-21 °C; (A5) saline-21 °C then NaOCl-60 °C; (A6) saline-60 °C then NaOCl-60 °C. A1-2 received nine 10-min irrigation periods (IP) with NaOCl; A3-6 received nine 10-min IP with saline, followed by 9 IP with NaOCl at different temperature combinations. Read More

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Myiasis in Neglected Cutaneous Squamous Cell Carcinoma of the Head and Neck: Review of Management and Current Protocol Recommendations.

Adv Skin Wound Care 2021 Jul;34(7):372-378

Golda Grinblat, MD, is Senior Consultant, Otolaryngology and Head and Neck Surgery Department, Hillel Yaffe Medical Center, Hadera, Israel. Yulia Frenkel, MD, is Resident, Anesthesiology Department, Sheba Medical Center, Tel Aviv. Itzhak Shochat, MD, is Resident, Otolaryngology and Head and Neck Surgery Department, Hillel Yaffe Medical Center. Andro Košec, MD, PhD, FEBORL-HNS, is Consultant Surgeon, Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Center Sestre Milosrdnice, School of Medicine, University of Zagreb, Croatia. Ayelet Midbari, MD, is Unit Chief, Pain Relief Unit, Hillel Yaffe Medical Center. Roman Rysin, MD, is Pre-Resident, Plastic Surgery Department, Hillel Yaffe Medical Center. Manjunath Dandinarasaiah, MD, is Associate Professor, Karnataka Institute of Medical Sciences, Vidyanagar, Hubli, Karnataka, India. At the Hillel Yaffe Medical Center, Itzhak Braverman, MD, is Director, Otolaryngology and Head and Neck Surgery Department; and Yoram Wolf, MD, is Unit Chief, Plastic Surgery Department. Acknowledgments: The authors thank Mrs Shelly Rado, Registered Nurse of the Pain Unit at Hillel Yaffe Medical Center for her assistance in wound care. The authors have disclosed no financial relationships related to this article. Submitted June 17, 2020; accepted in revised form October 2, 2020.

Objective: To propose a first-aid management protocol for myiasis in neglected cutaneous squamous cell carcinoma (SCC) in the ED based on a recent literature review.

Data Sources: PubMed.

Study Selection: Inclusion criteria were all series and case reports of primary/secondary cutaneous SCC with myiasis of the head and neck, including orbital SCC cases, published after 2005. Read More

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Supporting Clinicians to Use Technology to Deliver Highly Personalized and Measurement-Based Mental Health Care to Young People: Protocol for an Evaluation Study.

JMIR Res Protoc 2021 Jun 14;10(6):e24697. Epub 2021 Jun 14.

Brain and Mind Centre, University of Sydney, Camperdown, Australia.

Background: Australia's mental health care system has long been fragmented and under-resourced, with services falling well short of demand. In response, the World Economic Forum has recently called for the rapid deployment of smarter, digitally enhanced health services to facilitate effective care coordination and address issues of demand. The University of Sydney's Brain and Mind Centre (BMC) has developed an innovative digital health solution that incorporates 2 components: a highly personalized and measurement-based (data-driven) model of youth mental health care and a health information technology (HIT) registered on the Australian Register of Therapeutic Goods. Read More

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Establishment and long-term culture of mouse mammary stem cell organoids and breast tumor organoids.

STAR Protoc 2021 Jun 29;2(2):100577. Epub 2021 May 29.

Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Mammary stem cells (MaSCs) contribute to mammary epithelium development and homeostasis. They have been proposed as cells of origin for breast cancer. Here, we describe an organoid culture protocol for expansion of MaSCs from mouse tissues. Read More

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Using Expert Elicitation to Abridge the Welfare Quality® Protocol for Monitoring the Most Adverse Dairy Cattle Welfare Impairments.

Front Vet Sci 2021 28;8:634470. Epub 2021 May 28.

Animal Sciences Unit, Institute for Agricultural and Fisheries Research (ILVO), Merelbeke, Belgium.

The Welfare Quality® consortium has developed and proposed standard protocols for monitoring farm animal welfare. The uptake of the dairy cattle protocol has been below expectation, however, and it has been criticized for the variable quality of the welfare measures and for a limited number of measures having a disproportionally large effect on the integrated welfare categorization. Aiming for a wide uptake by the milk industry, we revised and simplified the Welfare Quality® protocol into a user-friendly tool for cost- and time-efficient on-farm monitoring of dairy cattle welfare with a minimal number of key animal-based measures that are aggregated into a continuous (and thus discriminative) welfare index (WI). Read More

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Rapamycin Improves the Response of Effector and Memory CD8 T Cells Induced by Immunization With ASP2 of .

Front Cell Infect Microbiol 2021 25;11:676183. Epub 2021 May 25.

Molecular Immunology Laboratory, Center of Molecular and Cellular Therapy, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.

Deficiency in memory formation and increased immunosenescence are pivotal features of infection proposed to play a role in parasite persistence and disease development. The vaccination protocol that consists in a prime with plasmid DNA followed by the boost with a deficient recombinant human adenovirus type 5, both carrying the ASP2 gene of , is a powerful strategy to elicit effector memory CD8 T-cells against this parasite. In virus infections, the inhibition of mTOR, a kinase involved in several biological processes, improves the response of memory CD8 T-cells. Read More

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The influence of natural vs anthropogenic factors on trace metal(loid) levels in the Mussel Watch programme: Two decades of monitoring in the Spanish Mediterranean sea.

Mar Environ Res 2021 Jun 9;169:105382. Epub 2021 Jun 9.

Instituto Español de Oceanografía (IEO), Centro Oceanográfico de Murcia, C/Varadero, 1, San Pedro del Pinatar, Murcia 30740, Spain.

The spatial distribution and temporal trends of trace metals (i.e. Cd, Cu, Hg, Pb and Zn) and a metalloid (i. Read More

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Cost, safety, and rehabilitation of same-stage, bilateral total knee replacements compared to two-stage total knee replacements.

Knee Surg Relat Res 2021 Jun 12;33(1):17. Epub 2021 Jun 12.

Department of Orthopedics & Traumatology, Prince of Wales Hospital, Sha Tin, Hong Kong SAR, China.

Background: Many patients experience bilateral knee osteoarthritis and require bilateral total knee replacement (TKR). Same-stage, bilateral TKR is proposed to be a cost-effective and safe solution compared to two-stage, but conflicting results in the literature are reported. We aim to compare the costs, safety, and rehabilitation performance of patients in same-stage versus two-stage, bilateral TKR with our centre's perioperative protocol. Read More

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Cognitive remediation therapy for anorexia nervosa as a rolling group intervention: Data from a longitudinal study in an eating disorders specialized inpatient unit.

Eur Eat Disord Rev 2021 Jun 12. Epub 2021 Jun 12.

Department of Neuroscience, University of Padova, Padova, Italy.

Objective: Cognitive remediation therapy (CRT) has been proposed as an add-on treatment approach that could increase the engagement in treatment of anorexia nervosa (AN) patients and reduce maintaining factors, but prior studies have evaluated CRT in individual and group settings, difficult protocols for rehabilitation settings. Our aim is to evaluate the CRT rolling protocol implementation in an inpatient specialised unit.

Methods: A historical longitudinal controlled study was designed to include 31 AN patients for the CRT program, and 28 AN patients treated as usual. Read More

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Analysis of clinical outcomes of oropharyngeal colostrum administration in very low-birth-weight preterm newborns.

Nutrition 2021 Apr 28;90:111292. Epub 2021 Apr 28.

Nutrition Institute Josué de Castro, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:

Objectives: Colostrum is the first secretion produced by the mammary glands and is present through the seventh day after birth. Colostrum has important immunomodulatory components and protective factors that contribute to the protection and development of newborns. The oropharyngeal administration of colostrum (OAC) has been proposed as a potential nutritional option for very low-birth-weight (VLBW) newborns (<1500 g). Read More

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Comparative efficacy and safety of metronomic chemotherapy in breast cancer: A protocol for network meta-analysis protocol.

Medicine (Baltimore) 2021 Jun;100(23):e26255

Department of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Dongcheng District, Beijing, China.

Background: Metronomic chemotherapy (MC) strategy has been used in breast cancer for more than a decade since it was first proposed. The purpose of this study is to systematically evaluate its efficacy and safety for breast cancer patients at various stages, as well as to clarify the most effective medication strategy when applying MC and discover its most sensitive subpopulation in breast cancer patients.

Method: We will systematically retrieve random controlled trials evaluating the efficacy and safety of MC in breast cancer on PubMed, Cochrane Library, Embase, and web of science to perform this network meta-analysis. Read More

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A double-closed wedge broken-line osteotomy for cubitus varus deformity.

Medicine (Baltimore) 2021 Jun;100(23):e26124

Department of Orthopedic Ward II, Shenzhen Children's Hospital, Shenzhen, China.

Abstract: Various osteotomy methods have been proposed in the treatment of cubitus varus. We designed an improved stepped osteotomy to achieve improved deformity correction. We refer to this new approach as double-closed wedge broken-line osteotomy and report a series of clinical and imaging results (deformity correction, range of motion [ROM], function, osteotomy healing, and complications) of patients with cubitus varus treated with this technique. Read More

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Experimental Demonstration of a Multifunctional All-Optical Quantum State Transfer Machine.

Phys Rev Lett 2021 May;126(21):210507

State Key Laboratory of Precision Spectroscopy, Joint Institute of Advanced Science and Technology, School of Physics and Electronic Science, East China Normal University, Shanghai 200062, China.

Quantum information protocol with quantum resources shows a great advantage in substantially improving security, fidelity, and capacity of information processing. Various quantum information protocols with diverse functionalities have been proposed and implemented. However, in general, the present quantum information system can only carry out a single information protocol or deal with a single communication task, which limits its practical application in the future. Read More

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Age-based inter-subject variability in platelet and white blood cell concentrations of platelet-rich plasma prepared using a new application to blood separation system.

Int Wound J 2021 Jun 11. Epub 2021 Jun 11.

Microbiology Section, Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain.

The benefit of autologous platelet-rich plasma (PRP) treatment is still under discussion. Variations in PRP products, consequence of the lack of a standardised protocol for the multitude of commercially available blood separation systems and the lack of knowledge of the optimal composition of PRP or its suitability for the proposed indication are some of the reasons behind clinical inconsistencies. The impact of inter-subject variability in PRP has received less attention in comparison. Read More

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Nasal Lavage Fluid as a Biomedical Sample for Verification of Chlorine Exposure.

J Anal Toxicol 2021 Jun 11. Epub 2021 Jun 11.

The Swedish Defence Research Agency, CBRN Defence and Security, Cementvägen 20, Umeå, Sweden.

Chlorine is a toxic chemical that has been used as a chemical warfare agent in recent armed conflicts. There is an urgent need for methods to verify alleged uses of chlorine, and phospholipid chlorohydrins (PL-HOCl) derived from the pulmonary surfactant of exposed victims have previously been proposed as biomarkers of chlorine exposure. Here we describe an improved protocol for the chemical analysis of these biomarkers and its applicability to biomedical samples from chlorine-exposed animals. Read More

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Emotion regulation training as a treatment element for externalizing problems in adolescence: A randomized controlled micro-trial.

Behav Res Ther 2021 May 26;143:103889. Epub 2021 May 26.

Department of Developmental Psychology, Utrecht University, 80125, 3508, TC, Utrecht, the Netherlands. Electronic address:

Improving interventions for externalizing problems in adolescence may require determining which treatment elements actually produce change. In this micro-trial, we tested a treatment element addressing one widely-hypothesized mechanism underlying externalizing problems: emotion regulation. We tested whether emotion regulation could be improved via training, whether adolescents who received such training would subsequently show reduced externalizing problems, and which training approach and sequence was most effective. Read More

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Predictive visuo-motor communication through neural oscillations.

Curr Biol 2021 Jun 3. Epub 2021 Jun 3.

Department of Translational Research on New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy; Laboratory of Medical Physics and Magnetic Resonance, IRCCS Stella Maris, Pisa, Italy. Electronic address:

The mechanisms coordinating action and perception over time are poorly understood. The sensory cortex needs to prepare for upcoming changes contingent on action, and this requires temporally precise communication that takes into account the variable delays between sensory and motor processing. Several theorists have proposed synchronization of the endogenous oscillatory activity observed in most regions of the brain as the basis for an efficient and flexible communication protocol between distal brain areas, a concept known as "communication through coherence. Read More

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Morally excused but socially excluded: Denying agency through the defense of mental impairment.

PLoS One 2021 10;16(6):e0252586. Epub 2021 Jun 10.

School of Psychological Sciences, University of Melbourne, Melbourne, Victoria, Australia.

Defendants can deny they have agency, and thus responsibility, for a crime by using a defense of mental impairment. We argue that although this strategy may help defendants evade blame, it may carry longer-term social costs, as lay people's perceptions of a person's agency might determine some of the moral rights they grant them. In this registered report protocol, we seek to expand upon preliminary findings from two pilot studies to examine how and why those using the defense of mental impairment are seen as less deserving of certain rights. Read More

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Prevalence of asymptomatic or "silent" myocardial ischemia in diabetic patients: Protocol for a systematic review and meta-analysis.

PLoS One 2021 10;16(6):e0252511. Epub 2021 Jun 10.

Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.

Introduction: Myocardial ischemia (MI) is a top ranked cause of death among diabetic patients, yet it is mostly asymptomatic or "silent". There is a need for summary epidemiologic measures on this highly lethal and unnoticeable complication of diabetes. The proposed systematic review and meta-analysis aims to estimate of the global prevalence of silent MI among diabetic patients. Read More

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Learned Gradient Compression for Distributed Deep Learning.

IEEE Trans Neural Netw Learn Syst 2021 Jun 10;PP. Epub 2021 Jun 10.

Training deep neural networks on large datasets containing high-dimensional data requires a large amount of computation. A solution to this problem is data-parallel distributed training, where a model is replicated into several computational nodes that have access to different chunks of the data. This approach, however, entails high communication rates and latency because of the computed gradients that need to be shared among nodes at every iteration. Read More

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Understanding the Effects of the Low-Concentration Electrolyte on the Performance of High-Energy-Density Li-S Batteries.

ACS Appl Mater Interfaces 2021 Jun 10. Epub 2021 Jun 10.

Institute for Superconducting & Electronic Materials (ISEM), Australian Institute of Innovative Materials (AIIM), Innovation Campus, University of Wollongong, Wollongong, NSW 2522, Australia.

High-energy-density Li-S batteries have been impeded by low power rate and low sulfur utilization of high-sulfur-loading cathode and unstable Li metal anode. Herein, a new method protocol was proposed to separately investigate the effects of low-concentration electrolytes on the cathode and the anode for Li-S batteries. It was found that 0. Read More

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CVAR-Seg: An Automated Signal Segmentation Pipeline for Conduction Velocity and Amplitude Restitution.

Front Physiol 2021 24;12:673047. Epub 2021 May 24.

Institute of Biomedical Engineering (IBT), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.

Background: Rate-varying S1S2 stimulation protocols can be used for restitution studies to characterize atrial substrate, ionic remodeling, and atrial fibrillation risk. Clinical restitution studies with numerous patients create large amounts of these data. Thus, an automated pipeline to evaluate clinically acquired S1S2 stimulation protocol data necessitates consistent, robust, reproducible, and precise evaluation of local activation times, electrogram amplitude, and conduction velocity. Read More

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Deep Cross-User Models Reduce the Training Burden in Myoelectric Control.

Front Neurosci 2021 24;15:657958. Epub 2021 May 24.

Department of Electrical and Computer Engineering, Institute of Biomedical Engineering, University of New Brunswick, Fredericton, NB, Canada.

The effort, focus, and time to collect data and train EMG pattern recognition systems is one of the largest barriers to their widespread adoption in commercial applications. In addition to multiple repetitions of motions, including exemplars of confounding factors during the training protocol has been shown to be critical for robust machine learning models. This added training burden is prohibitive for most regular use cases, so cross-user models have been proposed that could leverage inter-repetition variability supplied by other users. Read More

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Reweighting of molecular simulations with explicit-solvent SAXS restraints elucidates ion-dependent RNA ensembles.

Nucleic Acids Res 2021 Jun 9. Epub 2021 Jun 9.

Scuola Internazionale Superiore di Studi Avanzati, Via Bonomea 265, Trieste 34136, Italy.

Small-angle X-ray scattering (SAXS) experiments are increasingly used to probe RNA structure. A number of forward models that relate measured SAXS intensities and structural features, and that are suitable to model either explicit-solvent effects or solute dynamics, have been proposed in the past years. Here, we introduce an approach that integrates atomistic molecular dynamics simulations and SAXS experiments to reconstruct RNA structural ensembles while simultaneously accounting for both RNA conformational dynamics and explicit-solvent effects. Read More

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