34 results match your criteria propose collagen-based

Embedding Collagen in Multilayers for Enzyme-Assisted Mineralization: A Promising Way to Direct Crystallization in Confinement.

Biomacromolecules 2021 Jul 7. Epub 2021 Jul 7.

Laboratoire de Biomécanique and Bioingénierie, CNRS, Université de Technologie de Compiègne, BP 20529, F-60205 Compiègne Cedex, France.

The biogenic calcium phosphate (CaP) crystallization is a process that offers elegant materials design strategies to achieve bioactive and biomechanical challenges. Indeed, many biomimetic approaches have been developed for this process in order to produce mineralized structures with controlled crystallinity and shape. Herein, we propose an advanced biomimetic approach for the design of ordered hybrid mineralized nano-objects with highly anisotropic features. Read More

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Association of Systemic or Intravitreal Antivascular Endothelial Growth Factor (Anti-VEGF) and Impaired Wound Healing in Pediatric Patients: Collagen to the Rescue.

Vita Boyar

J Wound Ostomy Continence Nurs 2021 May-Jun 01;48(3):256-261

Vita Boyar, MD, Steven & Alexandra Cohen Children's Medical Center, New Hyde Park, New York; and Zucker School of Medicine at Hofstra/Northwell, New Hyde Park, New York.

Background: Bevacizumab is a humanized monoclonal antibody to vascular endothelial growth factor (VEGF) that has been used as a systemic chemotherapy treatment of various malignancies in adults since 2000. It has been used for pediatric patients over the last decade. In addition, bevacizumab is used for neonatal intravitreal administration for retinopathy of prematurity, a major complication of preterm birth, characterized by incomplete and abnormal vascularization of the retina that can lead to retinal detachment and blindness without treatment. Read More

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Marine Algae Incorporated Polylactide Acid Patch: Novel Candidate for Targeting Osteosarcoma Cells without Impairing the Osteoblastic Proliferation.

Polymers (Basel) 2021 Mar 10;13(6). Epub 2021 Mar 10.

Department of Oral and Maxillofacial Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.

Biodegradable collagen-based materials have been preferred as scaffolds and grafts for diverse clinical applications in density and orthopedy. Besides the advantages of using such bio-originated materials, the use of collagen matrices increases the risk of infection transmission through the cells or the tissues of the graft/scaffold. In addition, such collagen-based solutions are not counted as economically feasible approaches due to their high production cost. Read More

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A Tenon's capsule/bulbar conjunctiva interface biomimetic to model fibrosis and local drug delivery.

PLoS One 2020 3;15(11):e0241569. Epub 2020 Nov 3.

UCL Institute of Ophthalmology, UCL, London, United Kingdom.

Glaucoma filtration surgery is one of the most effective methods for lowering intraocular pressure in glaucoma. The surgery efficiently reduces intra-ocular pressure but the most common cause of failure is scarring at the incision site. This occurs in the conjunctiva/Tenon's capsule layer overlying the scleral coat of the eye. Read More

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December 2020

From Food Waste to Innovative Biomaterial: Sea Urchin-Derived Collagen for Applications in Skin Regenerative Medicine.

Mar Drugs 2020 Aug 6;18(8). Epub 2020 Aug 6.

Department of Environmental Science and Policy, University of Milan, Via Celoria, 2, 20133 Milan, Italy.

Collagen-based skin-like scaffolds (CBSS) are promising alternatives to skin grafts to repair wounds and injuries. In this work, we propose that the common marine invertebrate sea urchin represents a promising and eco-friendly source of native collagen to develop innovative CBSS for skin injury treatment. Sea urchin food waste after gonad removal was here used to extract fibrillar glycosaminoglycan (GAG)-rich collagen to produce bilayer (2D + 3D) CBSS. Read More

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Design and evaluation of collagen-inspired mineral-hydrogel nanocomposites for bone regeneration.

Acta Biomater 2020 08 1;112:262-273. Epub 2020 Jun 1.

Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15219; McGowan Institute for Regenerative Medicine, Pittsburgh, PA 15260; UPMC Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA 15260. Electronic address:

Bone loss due to trauma and tumors remains a serious clinical concern. Due to limited availability and disease transmission risk with autografts and allografts, calcium phosphate bone fillers and growth factor-based substitute bone grafts are currently used in the clinic. However, substitute grafts lack bone regeneration potential when used without growth factors. Read More

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Cuticle Collagen Expression Is Regulated in Response to Environmental Stimuli by the GATA Transcription Factor ELT-3 in .

Genetics 2020 06 30;215(2):483-495. Epub 2020 Mar 30.

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada L8S 4K1

The nematode is protected from the environment by the cuticle, an extracellular collagen-based matrix that encloses the animal. Over 170 cuticular collagens are predicted in the genome, but the role of each individual collagen is unclear. Stage-specific specialization of the cuticle explains the need for some collagens; however, the large number of collagens suggests that specialization of the cuticle may also occur in response to other environmental triggers. Read More

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Smad interacting protein 1 influences transforming growth factor-β/Smad signaling in extracellular matrix protein production and hypertrophic scar formation.

J Mol Histol 2019 Dec 8;50(6):503-514. Epub 2019 Oct 8.

Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, 710032, Shaanxi, China.

The transforming growth factor (TGF)-β/Smad signal transduction pathway is closely associated with hypertrophic scar (HS) formation. Smad interacting protein 1 (SIP1) is a cytoplasmic protein that efficiently regulates Smad2-/3-dependent signaling within the TGF-β pathway. SIP1 influences collagen synthesis in the HS through a heretofore unknown mechanism. Read More

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December 2019

Hand-held bioprinting for vascular formation applicable to dental pulp regeneration.

Connect Tissue Res 2020 03 9;61(2):205-215. Epub 2019 Jul 9.

Department of Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, RWTH Aachen University Hospital, Aachen, Germany.

Deep carious lesions may cause irreversible pulpitis and the current endodontic treatment typically removes the whole dental pulp tissue, which finally reduces lifespan of the teeth. Nowadays, the most frequent treatment is based on removing the infected tissue and filling the root canal with inert synthetic materials. Tissue engineering approaches are important alternatives to the current treatment, because they can potentially maintain the biological function of the tooth instead of sacrificing it. Read More

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YAP, ΔNp63, and β-Catenin Signaling Pathways Are Involved in the Modulation of Corneal Epithelial Stem Cell Phenotype Induced by Substrate Stiffness.

Cells 2019 04 12;8(4). Epub 2019 Apr 12.

Institute of Genetic Medicine, Newcastle University, International Centre for Life, Newcastle-upon-Tyne NE1 3BZ, UK.

Recent studies have established that the phenotype of epithelial stem cells residing in the corneal periphery (the limbus) depends on this niche's distinct biomechanical properties. However, the signaling pathways underlying this dependency are still poorly understood. To address this issue, we investigated the effect of substrate stiffness on the migration, proliferation, and molecular phenotype of human limbal epithelial stem cells (LESCs). Read More

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Corneal bioprinting utilizing collagen-based bioinks and primary human keratocytes.

J Biomed Mater Res A 2019 09 29;107(9):1945-1953. Epub 2019 Apr 29.

Department of Ophthalmology, RWTH Aachen University Hospital, Aachen, Germany.

Corneal transplantation is the treatment of choice for patients with advanced corneal diseases. However, the outcome may be affected by graft rejection, high associated costs, surgical expertise, and most importantly the worldwide donor shortage. In recent years, bioprinting has emerged as an alternative method for fabricating tissue equivalents using autologous cells with architecture resembling the native tissue. Read More

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September 2019

Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.

Mater Sci Eng C Mater Biol Appl 2019 Jul 26;100:236-246. Epub 2019 Feb 26.

CEITEC - Central European Institute of Technology, Brno University of Technology, Advanced Biomaterials, Purkynova 656/123, 612 00 Brno, Czech Republic.

Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties. Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented. Lipophilic BPL formed microspheres in porous scaffolds while reduced by half their swelling ratio. Read More

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Integration of and Models Using Bayesian Optimization With an Application to Stochastic Modeling of Mesenchymal 3D Cell Migration.

Front Physiol 2018 11;9:1246. Epub 2018 Sep 11.

Multiscale in Mechanical and Biological Engineering, Department of Mechanical Engineering, Aragón Institute of Engineering Research, Universidad de Zaragoza, Zaragoza, Spain.

Cellular migration plays a crucial role in many aspects of life and development. In this paper, we propose a computational model of 3D migration that is solved by means of the tau-leaping algorithm and whose parameters have been calibrated using Bayesian optimization. Our main focus is two-fold: to optimize the numerical performance of the mechano-chemical model as well as to automate the calibration process of models using Bayesian optimization. Read More

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September 2018

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model.

J Vis Exp 2018 02 7(132). Epub 2018 Feb 7.

Department Tissue Engineering and Regenerative Medicine, University Hospital Wuerzburg; Translational Center Regenerative Therapies (TLC-RT), Fraunhofer Institute for Silicate Research ISC.

Tissue engineering is a promising field, focused on developing solutions for the increasing demand on tissues and organs regarding transplantation purposes. The process to generate such tissues is complex, and includes an appropriate combination of specific cell types, scaffolds, and physical or biochemical stimuli to guide cell growth and differentiation. Microcarriers represent an appealing tool to expand cells in a three-dimensional (3D) microenvironment, since they provide higher surface-to volume ratios and mimic more closely the in vivo situation compared to traditional two-dimensional methods. Read More

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February 2018

Mesenchymal stem cell therapy for retro-corneal membrane - A clinical challenge in full-thickness transplantation of biosynthetic corneal equivalents.

Acta Biomater 2017 12 10;64:346-356. Epub 2017 Oct 10.

Section of Immunity, Infection and Inflammation, University of Aberdeen, Aberdeen, Scotland, United Kingdom. Electronic address:

Artificial corneas (keratoprostheses) and biosynthetic collagen-based corneal equivalents are surgical implants designed to ease the global burden of corneal blindness. However, keratoprostheses in many cases fail due to development of fibrous retro-corneal membranes (RCM). Fibrous membranes which develop in the anterior chamber after prosthesis implantation do so on a matrix of fibrin. Read More

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December 2017

Fabrication of a new physiological macroporous hybrid biomaterial/bioscaffold material based on polyphosphate and collagen by freeze-extraction.

J Mater Chem B 2017 Jun 26;5(21):3823-3835. Epub 2017 Apr 26.

ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, D-55128 Mainz, Germany.

We describe the fabrication of a new scaffold, an inorganic-organic hybrid biomaterial, consisting of the physiological polymers: the inorganic polymer polyphosphate (polyP), as well as the organic macromolecules collagen and chondroitin sulfate. The polyP polymer is composed of multiple phosphate orthophosphate units linked together by high-energy phosphoanhydride bonds. Chondroitin sulfate has been included due to its hydrogel-forming properties. Read More

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A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models.

Ann Biomed Eng 2017 06 21;45(6):1496-1510. Epub 2017 Feb 21.

μBS Lab, Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, P.zza Leonardo da Vinci 32, 20133, Milan, Italy.

In the past decades, vascular tissue engineering has made great strides towards bringing engineered vascular tissues to the clinics and, in parallel, obtaining in-lab tools for basic research. Herein, we propose the design of a novel dual-mode bioreactor, useful for the fabrication (construct mode) and in vitro stimulation (culture mode) of collagen-based tubular constructs. Collagen-based gels laden with smooth muscle cells (SMCs) were molded directly within the bioreactor culture chamber. Read More

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A collagen cardiac patch incorporating alginate microparticles permits the controlled release of hepatocyte growth factor and insulin-like growth factor-1 to enhance cardiac stem cell migration and proliferation.

J Tissue Eng Regen Med 2018 01 19;12(1):e384-e394. Epub 2017 Jun 19.

Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland.

Cardiac stem cells (CSCs) represent a logical cell type to exploit as a regenerative treatment option for tissue damage accrued as a result of a myocardial infarction. However, the isolation and expansion of CSCs prior to cell transplantation is time consuming, costly and invasive, and the reliability of cell expansion may also prove to be a major obstacle in the clinical application of CSC-based transplantation therapy after a myocardial infarction. In order to overcome this, we propose the incorporation of growth factor-eluting alginate microparticles into collagen-based scaffolds as an implantable biomaterial to promote the recruitment and expansion of CSCs in the myocardium. Read More

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January 2018

Strategy to Achieve Highly Porous/Biocompatible Macroscale Cell Blocks, Using a Collagen/Genipin-bioink and an Optimal 3D Printing Process.

ACS Appl Mater Interfaces 2016 Nov 17;8(47):32230-32240. Epub 2016 Nov 17.

Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU) , Suwon, South Korea.

Recently, a three-dimensional (3D) bioprinting process for obtaining a cell-laden structure has been widely applied because of its ability to fabricate biomimetic complex structures embedded with and without cells. To successfully obtain a cell-laden porous block, the cell-delivering vehicle, bioink, is one of the significant factors. Until now, various biocompatible hydrogels (synthetic and natural biopolymers) have been utilized in the cell-printing process, but a bioink satisfying both biocompatibility and print-ability requirements to achieve a porous structure with reasonable mechanical strength has not been issued. Read More

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November 2016

Fundamental insight into the effect of carbodiimide crosslinking on cellular recognition of collagen-based scaffolds.

Acta Biomater 2017 02 30;49:218-234. Epub 2016 Nov 30.

Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, United Kingdom.

Research on the development of collagen constructs is extremely important in the field of tissue engineering. Collagen scaffolds for numerous tissue engineering applications are frequently crosslinked with 1-ethyl-3-(3-dimethylaminopropyl-carbodiimide hydrochloride (EDC) in the presence of N-hydroxy-succinimide (NHS). Despite producing scaffolds with good biocompatibility and low cellular toxicity the influence of EDC/NHS crosslinking on the cell interactive properties of collagen has been overlooked. Read More

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February 2017

Featured Article: Nanoenhanced matrix metalloproteinase-responsive delivery vehicles for disease resolution and imaging.

Exp Biol Med (Maywood) 2016 12 29;241(18):2023-2032. Epub 2016 Jul 29.

1 SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering, Albany, NY 12203, USA.

The wide array of proteases, including matrix metalloproteinases, produced in response to many pathogenic insults, confers a unique proteolytic signature which is often disease specific and provides a potential therapeutic target for drug delivery. Here we propose the use of collagen-based nanoenhanced matrix metalloproteinase-responsive delivery vehicles that display matrix metalloproteinase-specific degradation in diverse in vitro models of proteolysis. We demonstrate that collagen particles comprised of protease substrates (primarily collagen) can be made of uniform size and loaded efficiently with assorted cargo including fluorescently labeled mesoporous silica, magnetic nanoparticles, proteins and antioxidants. Read More

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December 2016

Analysis and quantification of collagen organization with the structure tensor in second harmonic microscopy images of ocular tissues.

Appl Opt 2015 Nov;54(33):9848-54

The important biological role of collagen-based tissues and the changes produced in the fiber distribution under particular situations (surgery, pathology, external damage, etc.) require tools for the analysis of the collagen organization that might potentially help in early diagnoses. Since collagen structures provide efficient second harmonic generation (SHG) signals, SHG microscopy has emerged as a powerful technique to visualize collagen fibers and qualitatively discriminate normal from abnormal tissues. Read More

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November 2015

Novel therapeutic core-shell hydrogel scaffolds with sequential delivery of cobalt and bone morphogenetic protein-2 for synergistic bone regeneration.

Acta Biomater 2015 Sep 6;23:295-308. Epub 2015 Jun 6.

Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea; Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 330-714, South Korea. Electronic address:

Unlabelled: Enabling early angiogenesis is a crucial issue in the success of bone tissue engineering. Designing scaffolds with therapeutic potential to stimulate angiogenesis as well as osteogenesis is thus considered a promising strategy. Here, we propose a novel scaffold designed to deliver angiogenic and osteogenic factors in a sequential manner to synergize the bone regeneration event. Read More

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September 2015

Cell morphology and focal adhesion location alters internal cell stress.

J R Soc Interface 2014 Dec;11(101):20140885

Centre for Biomechanics Research (BMEC), Department of Biomedical Engineering, NUI Galway, Galway, Republic of Ireland National Centre for Biomedical Engineering Science (NCBES), NUI Galway, Galway, Republic of Ireland

Extracellular mechanical cues have been shown to have a profound effect on osteogenic cell behaviour. However, it is not known precisely how these cues alter intracellular mechanics to initiate changes in cell behaviour. In this study, a combination of in vitro culture of MC3T3-E1 cells and finite-element modelling was used to investigate the effects of passive differences in substrate stiffness on intracellular mechanics. Read More

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December 2014

Reversible stress softening of collagen based networks from the jumbo squid mantle (Dosidicus gigas).

Mater Sci Eng C Mater Biol Appl 2014 Apr 27;37:9-13. Epub 2013 Dec 27.

Instituto de Ciencia y Tecnología de Polímeros, C.S.I.C., Calle Juan de la Cierva 3, 28006 Madrid, Spain.

Dosidicus gigas is the largest and one of the most abundant jumbo squids in the eastern Pacific Ocean. In this paper we have studied the muscle of the mantle of D. gigas (DGM). Read More

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Heparanase-mediated cleavage of macromolecular heparin accelerates release of granular components of mast cells from extracellular matrices.

Biochem J 2014 Mar;458(2):291-9

*Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.

Heparanase cleaves macromolecular heparin in the secretory granules of connective tissue-type mast cells. We investigated roles of the cleavage under a microenvironment mimicking where the mast cells physiologically reside. A connective tissue-type mast cell line MST and mouse peritoneal cell-derived mast cells stored macromolecular heparin in the secretory granules. Read More

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Osteocyte differentiation is regulated by extracellular matrix stiffness and intercellular separation.

J Mech Behav Biomed Mater 2013 Dec 18;28:183-94. Epub 2013 Jul 18.

Centre for Biomechanics Research (BMEC), Mechanical and Biomedical Engineering, NUI Galway, Ireland; National Centre for Biomedical Engineering Science (NCBES), NUI Galway, Ireland.

Osteocytes are terminally differentiated bone cells, derived from osteoblasts, which are vital for the regulation of bone formation and resorption. ECM stiffness and cell seeding density have been shown to regulate osteoblast differentiation, but the precise cues that initiate osteoblast-osteocyte differentiation are not yet understood. In this study, we cultured MC3T3-E1 cells on (A) substrates of different chemical compositions and stiffnesses, as well as, (B) substrates of identical chemical composition but different stiffnesses. Read More

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December 2013

Viscoelastic properties of model segments of collagen molecules.

Matrix Biol 2012 Mar 21;31(2):141-9. Epub 2011 Dec 21.

Laboratory for Atomistic and Molecular Mechanics, Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Room 1-235A&B, Cambridge, MA, USA.

Collagen is the prime construction material in vertebrate biology, determining the mechanical behavior of connective tissues such as tendon, bone and skin. Despite extensive efforts in the investigation of the origin of collagen unique mechanical properties, a deep understanding of the relationship between molecular structure and mechanical properties remains elusive, hindered by the complex hierarchical structure of collagen-based tissues. In particular, although extensive studies of viscoelastic properties have been pursued at the macroscopic (fiber/tissue) level, fewer investigations have been performed at the smaller scales, including in particular collagen molecules and fibrils. Read More

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3D co-culture of hematopoietic stem and progenitor cells and mesenchymal stem cells in collagen scaffolds as a model of the hematopoietic niche.

Biomaterials 2012 Feb 1;33(6):1736-47. Epub 2011 Dec 1.

Institute of Pathology, RWTH Aachen University, Medical School, Pauwelsstrasse 30, 52074 Aachen, Germany.

Here, we propose a collagen-based three-dimensional (3D) environment for hematopoietic stem and progenitor cells (HPC) with mesenchymal stem cells (MSC) derived either from bone marrow (BM) or umbilical cord (UC), to recapitulate the main components of the BM niche. Mechanisms described for HPC homeostasis were systematically analyzed in comparison to the conventional liquid HPC culture. The 3D-cultivation allows dissecting two sub-populations of HPC: (I) HPC in suspension above the collagen gel and (II) migratory HPC in the collagen fibres of the collagen gel. Read More

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February 2012

Osteogenic differentiation of human Wharton's jelly stem cells on nanofibrous substrates in vitro.

Tissue Eng Part A 2011 Jan 9;17(1-2):71-81. Epub 2010 Sep 9.

Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine National University of Singapore, Singapore, Singapore.

Most tissue engineering studies use human bone marrow mesenchymal stem cells for differentiation into desirable lineages. We derived a novel stem cell from the human umbilical cord Wharton's jelly (hWJSC) that has numerous advantages over other stem cell types in that they can be harvested in abundance very efficiently and painlessly with no risk of patient morbidity, have prolonged stemness properties in vitro, are hypoimmunogenic, and can be differentiated into many tissue types in two-dimensional culture. We compared four different three-dimensional nanofibrous scaffolds (polycaprolactone [PCL], PCL/collagen [PCL/Coll], PCL/hydroxyapatite [PCL/HA], and PCL/Coll/HA) for the attachment, proliferation, differentiation, and mineralization of hWJSCs into an osteogenic lineage. Read More

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January 2011