87,364 results match your criteria promoter activity

P300 inhibition improves cell apoptosis and cognition impairment induced by sevoflurane through regulating IL-17A activation.

World Neurosurg 2021 Jul 24. Epub 2021 Jul 24.

Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin.

Sevoflurane (Sev) is a rapidly acting, potent inhalation anesthetic with rapid uptake and elimination. But many studies showed Sev could result in cognition dysfunction in adolescent or aging patients. Now, therapeutic targeting with IL-17A antibody showed a promising effect on Sev induced cognition impairment. Read More

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Pharmacological evidence for the concept of spare glutamate transporters.

Neurochem Int 2021 Jul 24:105142. Epub 2021 Jul 24.

Institute of Neuroscience, Université Catholique de Louvain, Avenue Hippocrate B1.54.10, 1200, Brussels, Belgium. Electronic address:

Through the efficient clearance of extracellular glutamate, high affinity astrocytic glutamate transporters constantly shape excitatory neurotransmission in terms of duration and spreading. Even though the glutamate transporter GLT-1 (also known as EAAT2/SLC1A2) is amongst the most abundant proteins in the mammalian brain, its density and activity are tightly regulated. In order to study the influence of changes in the expression of GLT-1 on glutamate uptake capacity, we have developed a model in HEK cells where the density of the transporter can be manipulated thanks to a tetracycline-inducible promoter. Read More

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Genome-Scale Analysis of Acetobacterium woodii Identifies Translational Regulation of Acetogenesis.

mSystems 2021 Jul 27:e0069621. Epub 2021 Jul 27.

Department of Biological Sciences, Korea Advanced Institute of Science and Technologygrid.37172.30, Daejeon, Republic of Korea.

Acetogens synthesize acetyl-CoA via the CO-fixing Wood-Ljungdahl pathway. Despite their ecological and biotechnological importance, their translational regulation of carbon and energy metabolisms remains unclear. Here, we report how carbon and energy metabolisms in the model acetogen Acetobacterium woodii are translationally controlled under different growth conditions. Read More

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Ligands and DNA in the allosteric control of retinoid receptors function.

Essays Biochem 2021 Jul 23. Epub 2021 Jul 23.

Centre de Biologie Structurale (CBS), Inserm, CNRS, Univ Montpellier, Montpellier, France.

Retinoids are a family of compounds that include both vitamin A (all-trans retinol) and its naturally occurring metabolites such as retinoic acids (e.g. all-trans retinoic acid) as well as synthetic analogs. Read More

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Heterotopic ossification in mice overexpressing Bmp2 in Tie2+ lineages.

Cell Death Dis 2021 Jul 22;12(8):729. Epub 2021 Jul 22.

Intercellular Signaling in Cardiovascular Development & Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro 3, 28029, Madrid, Spain.

Bone morphogenetic protein (Bmp) signaling is critical for organismal development and homeostasis. To elucidate Bmp2 function in the vascular/hematopoietic lineages we generated a new transgenic mouse line in which ectopic Bmp2 expression is controlled by the Tie2 promoter. Tie2;Bmp2 mice develop aortic valve dysfunction postnatally, accompanied by pre-calcific lesion formation in valve leaflets. Read More

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IκB kinase-β regulates neutrophil recruitment through activation of STAT3 signaling in the esophagus.

Cell Mol Gastroenterol Hepatol 2021 Jul 23. Epub 2021 Jul 23.

Department of Medicine, Gastroenterology and Hepatology Division, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. Electronic address:

Background & Aims: The epithelial barrier is the host's first line of defense against damage to the underlying tissue. Upon injury, the epithelium plays a critical role in inflammation. The IKKβ/NF-κB pathway was shown to be active in the esophageal epithelium of patients with esophageal disease. Read More

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Manganese-induced reactive oxygen species activate IκB kinase to upregulate YY1 and impair glutamate transporter EAAT2 function in human astrocytes in vitro.

Neurotoxicology 2021 Jul 23. Epub 2021 Jul 23.

Department of Pharmaceutical Sciences, College of Pharmacy, Florida A&M University, Tallahassee, FL, 32301, USA. Electronic address:

Dysregulation of the astrocytic glutamate transporter excitatory amino acid transporter 2 (EAAT2) is associated with several neurological disorders, including Parkinson's disease, Alzheimer's disease, and manganism, the latter induced by chronic exposure to high levels of manganese (Mn). Mechanisms of Mn-induced neurotoxicity include impairment in EAAT2 function secondary to the activation of the transcription factor Yin Yang 1 (YY1) by nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). However, the upstream mechanisms by which Mn-induced NF-κB activates YY1 remain has yet to be elucidated. Read More

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A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.

PLoS One 2021 26;16(7):e0254802. Epub 2021 Jul 26.

Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.

Spermatozoa released from testes undergo a maturation process and acquire the capacity to fertilize ova through epididymal transit. The epididymis is divided into four regions, each with unique morphology, gene profile, luminal microenvironment and distinct function. To study the functions of relevant genes in the epididymal initial segment (IS), a novel IS-specific mouse model, Lcn9-Cre knock-in (KI) mouse line was generated via CRISPR/Cas9 technology. Read More

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Binding Studies of Aloe-Active Compounds with G-Quadruplex Sequences.

ACS Omega 2021 Jul 9;6(28):18344-18351. Epub 2021 Jul 9.

Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India.

G-quadruplex, a unique DNA quartet motif with a pivotal role in regulation of the gene expression, has been established as a potent therapeutic target for the treatment of cancer. Small-molecule-mediated stabilization of the G-quadruplex and thus inhibition of the expression from the oncogene promoter and telomere region may be a promising anticancer strategy. -derived natural compounds like aloe emodin, aloe emodin-8-glucoside, and aloin have significant anticancer activity. Read More

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DING Protein Inhibits Transcription of HIV-1 Gene through Suppression of Phosphorylation of NF-κB p65.

J HIV AIDS 2020 Aug 31;6(1). Epub 2020 Aug 31.

Department of Biology, College of Science and Technology, Temple University, Philadelphia, USA.

Introduction: Novel plant DING proteins (full-length 38 kDa p38SJ, and 27 kDa p27SJ) exhibit phosphatase activity and modulate HIV-1 gene transcription. Previously, we demonstrated that DING regulates HIV-1 gene transcription by dephosphorylation and inactivation of CTD RNA polymerase II, the major elongating factor of HIV-1 Long Terminal Repeats (LTR). Because the transcription of HIV-1 is controlled by several viral and cellular factors, including p65/p50 subunits of NF-κB, we hypothesized that DING phosphatase can also affect the phosphorylation and activity of p65 NF-κB, in addition to C-terminal Domain (CTD) of RNA Polymerase II (RNAPII), to suppress HIV-1 gene transcription and inhibit HIV-1 infection. Read More

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Bimetallic Ni-Ru and Ni-Re Catalysts for Dry Reforming of Methane: Understanding the Synergies of the Selected Promoters.

Front Chem 2021 7;9:694976. Epub 2021 Jul 7.

Centro Mixto Universidad de Sevilla-CSIC, Instituto de Ciencia de Materiales de Sevilla, Sevilla, Spain.

Designing an economically viable catalyst that maintains high catalytic activity and stability is the key to unlock dry reforming of methane (DRM) as a primary strategy for biogas valorization. Ni/AlO catalysts have been widely used for this purpose; however, several modifications have been reported in the last years in order to prevent coke deposition and deactivation of the samples. Modification of the acidity of the support and the addition of noble metal promoters are between the most reported strategies. Read More

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FOXP3 Inhibits the Metastasis of Breast Cancer by Downregulating the Expression of MTA1.

Front Oncol 2021 7;11:656190. Epub 2021 Jul 7.

State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi'an, China.

Background: FOXP3, as a tumour suppressor gene, has a vital function in inhibiting the metastasis of breast cancer cells, but the mechanisms by which it inhibits metastasis have not been fully elucidated. This study intended to explore a new mechanism by which FOXP3 inhibits breast cancer metastasis.

Methods: Bioinformatic analysis was performed to identify potential downstream molecules of FOXP3. Read More

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Human Cytomegalovirus UL23 Attenuates Signal Transducer and Activator of Transcription 1 Phosphorylation and Type I Interferon Response.

Front Microbiol 2021 9;12:692515. Epub 2021 Jul 9.

Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, China.

Human cytomegalovirus (HCMV), the human beta-herpesvirus, can cause severe syndromes among both immunocompromised adult patients and newborns. Type I interferon (IFN-I) exerts an important effect to resist infections caused by viruses such as HCMV, while IFN evasion may serve as a key determining factor for viral dissemination and disease occurrence within hosts. In this study, UL23, a tegument protein of HCMV, was confirmed to be a key factor for negatively regulating the type I IFN immune response. Read More

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Pan-cancer analysis of clinical significance and associated molecular features of glycolysis.

Bioengineered 2021 Dec;12(1):4233-4246

Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Tumor glycolysis is a major promoter of carcinogenesis and cancer progression. Given its complex mechanisms and interactions, comprehensive analysis is needed to reveal its clinical significance and molecular features. On the basis of a well-established glycolysis gene expression signature, we quantified 8633 patients with different cancer types from the Cancer Genome Atlas (TCGA) and evaluated their prognostic associations. Read More

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December 2021

A cell engineering approach to enzyme-based fed-batch fermentation.

Microb Cell Fact 2021 Jul 24;20(1):146. Epub 2021 Jul 24.

Department of Biochemical Engineering, UCL, Gower Street, London, WC1E 6BT, UK.

Background: A fundamental problem associated with E. coli fermentations is the difficulty in achieving high cell densities in batch cultures, attributed in large part to the production and accumulation of acetate through a phenomenon known as overflow metabolism when supplying enough glucose for the cell density desired. Although a fed-batch configuration is the standard method for reducing such issues, traditional fed-batch systems require components which become problematic when applying them at smaller scale. Read More

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Discovery and characterization of novel peptide inhibitors of the NRF2/MAFG/DNA ternary complex for the treatment of cancer.

Eur J Med Chem 2021 Jul 7;224:113686. Epub 2021 Jul 7.

Merck & Co., Inc., Chemistry, 33 Avenue Louis Pasteur, Boston, MA 02127, USA.

Pathway activating mutations of the transcription factor NRF2 and its negative regulator KEAP1 are strongly correlative with poor clinical outcome with pemetrexed/carbo(cis)platin/pembrolizumab (PCP) chemo-immunotherapy in lung cancer. Despite the strong genetic support and therapeutic potential for a NRF2 transcriptional inhibitor, currently there are no known direct inhibitors of the NRF2 protein or its complexes with MAF and/or DNA. Herein we describe the design of a novel and high-confidence homology model to guide a medicinal chemistry effort that resulted in the discovery of a series of peptides that demonstrate high affinity, selective binding to the Antioxidant Response Element (ARE) DNA and thereby displace NRF2-MAFG from its promoter, which is an inhibitory mechanism that to our knowledge has not been previously described. Read More

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The Inflammatory Regulation of TRPA1 Expression in Human A549 Lung Epithelial Cells.

Pulm Pharmacol Ther 2021 Jul 21:102059. Epub 2021 Jul 21.

The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, Tampere, Finland. Electronic address:

Transient receptor potential ankyrin-1 (TRPA1) is an ion channel mediating pain and cough signals in sensory neurons. We and others have shown that TRPA1 is also expressed in some non-neuronal cells and supports inflammatory responses. To address the pathogenesis and to uncover potential targets for pharmacotherapy in inflammatory lung diseases, we set out to study the expression of TRPA1 in human A549 lung epithelial cells under inflammatory conditions. Read More

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The lncRNA HMS recruits RNA binding protein HuR to stabilize the 3'-UTR of HOXC10 mRNA.

J Biol Chem 2021 Jul 21:100997. Epub 2021 Jul 21.

National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi-110067, India; JNU, New Delhi, India. Electronic address:

Long non-coding RNAs (lncRNAs) have been reported to drive key cancer pathways but the functions of majority of lncRNAs are unknown making a case for comprehensive functional evaluation of lncRNAs. With an aim to identify lncRNAs dysregulated in human cancers we analyzed the cancer patient database of lung adenocarcinoma (LUAD) which revealed an upregulated lncRNA, LINC02381 (renamed HOXC10mRNA stabilizing factor or HMS in this study), whose depletion results in proliferation defects and inhibition of colony formation of human cancer cells. In order to identify the binding targets of HMS, we screened for cis-genes and discovered that HOXC10, an oncogene, is downregulated in the absence of HMS. Read More

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Immediate-early transcriptional response to insulin receptor stimulation.

Biochem Pharmacol 2021 Jul 21:114696. Epub 2021 Jul 21.

Department of Medical Biochemistry and Molecular Biology, Saarland University Medical Faculty, D-66421 Homburg, Germany.

Insulin binding to the insulin receptor triggers intracellular signaling cascades involving the activation of protein and lipid kinases. As a result, multiple biological functions of the cells are changed. Here, we analyzed the regulation and signaling cascades leading to insulin-induced activation of the stimulus-responsive transcription factors. Read More

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Cannabidiol disrupts apoptosis, autophagy and invasion processes of placental trophoblasts.

Arch Toxicol 2021 Jul 24. Epub 2021 Jul 24.

UCIBIO.REQUIMTE, Faculty of Pharmacy, Biochemistry Lab, Biological Sciences Department, University of Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313, Porto, Portugal.

Cannabidiol (CBD) is a constituent of Cannabis sativa without psychotropic activity, whose medical benefits have been recognised. However, little is known about the potential toxic effects of CBD on reproductive health. Placental development involves tightly controlled processes of cell proliferation, differentiation, apoptosis, autophagy and migration/invasion of trophoblast cells. Read More

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SMYD3 promotes hepatocellular carcinoma progression by methylating S1PR1 promoters.

Cell Death Dis 2021 Jul 23;12(8):731. Epub 2021 Jul 23.

Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 510120, Guangzhou, China.

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. SET and MYND domain-containing protein 3 (SMYD3) has been shown to promote the progression of various types of human cancers, including liver cancer; however, the detailed molecular mechanism is still largely unknown. Here, we report that SMYD3 expression in HCC is an independent prognostic factor for survival and promotes the proliferation and migration of HCC cells. Read More

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EZH2 inhibits NK cell-mediated antitumor immunity by suppressing CXCL10 expression in an HDAC10-dependent manner.

Proc Natl Acad Sci U S A 2021 Jul;118(30)

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35233;

Enhancer of zeste homolog 2 (EZH2) is a histone H3 lysine 27 methyltransferase that has been shown to function as an oncogene in some cancers. Previous reports have largely focused on the ability of EZH2 to regulate cell-intrinsic tumor regulatory pathways as its mechanism-of-oncogenic action. However, the role that EZH2-mediated immune suppression plays in its oncogenic activity is not fully known. Read More

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HPV Infection Leaves a DNA Methylation Signature in Oropharyngeal Cancer Affecting Both Coding Genes and Transposable Elements.

Cancers (Basel) 2021 Jul 20;13(14). Epub 2021 Jul 20.

Molecular Carcinogenesis Program, Brazilian National Cancer Institute, Rio de Janeiro CEP 20231-050, Brazil.

HPV oncoproteins can modulate DNMT1 expression and activity, and previous studies have reported both gene-specific and global DNA methylation alterations according to HPV status in head and neck cancer. However, validation of these findings and a more detailed analysis of the transposable elements (TEs) are still missing. Here we performed pyrosequencing to evaluate a 5-CpG methylation signature and Line1 methylation in an oropharyngeal squamous cell carcinoma (OPSCC) cohort. Read More

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A plant CitPITP1 protein-coding exon sequence serves as a promoter in bacteria.

J Biotechnol 2021 Jul 20. Epub 2021 Jul 20.

Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan, 430000, China. Electronic address:

Genetic manipulation of plant genes in prokaryotes has been widely used in molecular biology, but the function of a DNA sequence is far from being fully known. Here, we discovered that a plant protein-coding gene containing the CRAL_TRIO domain serves as a promoter in bacteria. We firstly characterized CitPITP1 from Citrus, which contains the CRAL_TRIO domain, and identified a 64-bp sequence (key64) that is critical for prokaryotic promoter activity. Read More

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Lysine demethylase KDM6B regulates HIF-1α mediated systemic and cellular responses to intermittent hypoxia.

Physiol Genomics 2021 07 23. Epub 2021 Jul 23.

Institute for Integrative Physiology and Center for Systems Biology of O2 Sensing, University of Chicago, Chicago, IL, United States.

Intermittent hypoxia (IH) is a hallmark manifestation of Obstructive Sleep Apnea (OSA). Rodents treated with IH exhibit hypertension. Hypoxia-inducible factor (HIF)-1-dependent transcriptional activation of NADPH oxidases (Nox) and the resulting increase in reactive oxygen species (ROS) levels is a major molecular mechanism underlying IH/OSA-induced hypertension. Read More

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Oxidative stress in obesity-associated hepatocellular carcinoma: sources, signaling and therapeutic challenges.

Oncogene 2021 Jul 21. Epub 2021 Jul 21.

Signal Transduction and Metabolism Laboratory, Laboratoire de Gastroentérologie Expérimental et Endotools, Université libre de Bruxelles, Brussels, Belgium.

Obesity affects more than 650 million individuals worldwide and is a well-established risk factor for the development of hepatocellular carcinoma (HCC). Oxidative stress can be considered as a bona fide tumor promoter, contributing to the initiation and progression of liver cancer. Indeed, one of the key events involved in HCC progression is excessive levels of reactive oxygen species (ROS) resulting from the fatty acid influx and chronic inflammation. Read More

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Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.

PLoS One 2021 21;16(7):e0248607. Epub 2021 Jul 21.

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States of America.

Microbial colonization of plant roots is a highly complex process that requires the coordination and regulation of many gene networks, yet the identities and functions of many of these gene products have yet to be discovered. Pantoea sp. YR343, a gamma-proteobacterium isolated from the rhizosphere of Populus deltoides, forms robust biofilms along the root surfaces of Populus and possesses plant growth-promoting characteristics. Read More

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WT1 inhibits AML cell proliferation in a p53-dependent manner.

Cell Cycle 2021 Jul 21:1-9. Epub 2021 Jul 21.

Shanghai Ninth People's Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.

WT1 has been reported to function as an oncogene and a tumor suppressor in acute myeloid leukemia (AML). The molecular mechanisms have not yet been fully elucidated. Here, we report that p53, served as a tumor suppressor, plays a critical role in regulating the function of WT1 in AML. Read More

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Association analysis and functional study of COL6A1 single nucleotide polymorphisms in thoracic ossification of the ligamentum flavum in the Chinese Han population.

Eur Spine J 2021 Jul 21. Epub 2021 Jul 21.

Department of Orthopaedics, Peking University Third Hospital, Beijing, People's Republic of China.

Purpose: Genetic factors play a crucial role in thoracic ossification of the ligamentum flavum (TOLF). This study aimed to better understand the association between single nucleotide polymorphisms (SNP) in functional regions of the collagen VI, alpha 1 gene (COL6A1) and TOLF, and to confirm COL6A1 as a TOLF susceptibility gene.

Methods: Ten tag SNPs in COL6A1 were genotyped using the SNaPshot assay, and allele and genotype frequencies were compared between TOLF patients and control individuals. Read More

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MYB mediates downregulation of the colorectal cancer metastasis suppressor HNRNPLL during epithelial-mesenchymal transition.

Cancer Sci 2021 Jul 19. Epub 2021 Jul 19.

Division of Pathophysiology, Aichi Cancer Center Research Institute, Nagoya, Japan.

HNRNPLL (heterogeneous nuclear ribonucleoprotein L-like) , a suppressor of colorectal cancer (CRC) metastasis, is transcriptionally downregulated when CRC cells undergo epithelial-mesenchymal transition (EMT). Here we show that decrease of MYB mediates the downregulation of HNRNPLL during EMT. The promoter activity was attributed to a region from -273 to -10 base pairs upstream of the transcription start site identified by 5'-RACE analysis, and the region contained potential binding sites for MYB and SP1. Read More

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