20,071 results match your criteria profiling activation

B cells acquire a unique and differential transcriptomic profile during pregnancy.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

Center for Pharmacological and Botanical Studies (CEFYBO-UBA-CONICET), Medical Faculty, Buenos Aires University, Buenos Aires, Argentina; Centro Integrativo de Biología Y Química Aplicada, Universidad Bernardo O'Higgins, 8307993 Santiago, Chile. Electronic address:

Pregnancy alters B cell development and function. B cell activation is initiated by antigens binding to the BCR leading to B cell survival, proliferation, antigen presentation and antibody production. We performed a genome-wide transcriptome profiling of splenic B cells from pregnant (P) and non-pregnant (NP) mice and identified 1136 genes exhibiting differential expression in B cells from P mice (625 up- and 511 down-regulated) compared to NP animals. Read More

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Expression profiling, immune functions, and molecular characteristics of the tetraspanin molecule CD63 from Amphiprion clarkii.

Dev Comp Immunol 2021 Jun 9:104168. Epub 2021 Jun 9.

Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea. Electronic address:

CD63, a member of the tetraspanin family, is involved in the activation of immune cells, antiviral immunity, and signal transduction. The economically important anemonefishes Amphiprion sp. often face disease outbreaks, and the present study aimed to characterize CD63 in Amphiprion clarkii (denoted AcCD63) to enable better disease management. Read More

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Multimodal analysis for human studies shows extensive molecular changes from delays in blood processing.

iScience 2021 May 15;24(5):102404. Epub 2021 Apr 15.

Allen Institute for Immunology, Seattle, WA 98109, USA.

Multi-omic profiling of human peripheral blood is increasingly utilized to identify biomarkers and pathophysiologic mechanisms of disease. The importance of these platforms in clinical and translational studies led us to investigate the impact of delayed blood processing on the numbers and state of peripheral blood mononuclear cells (PBMC) and on the plasma proteome. Similar to previous studies, we show minimal effects of delayed processing on the numbers and general phenotype of PBMC up to 18 hours. Read More

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Human Gastric Cancer Stem Cell (GCSC) Markers Are Prognostic Factors Correlated With Immune Infiltration of Gastric Cancer.

Front Mol Biosci 2021 25;8:626966. Epub 2021 May 25.

Department of Science and Education, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, China.

The prognosis of patients with gastric cancer (GC) is still unsatisfying. Numerous markers of gastric cancer stem cells (GCSCs) have been identified and were thought to be related to cancer aggressiveness. However, the roles of GCSC markers in GC patients' prognosis and immune infiltration remain unknown. Read More

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Overexpression of a -Related Gene Promotes Bud Break in Ecodormant Poplars.

Front Plant Sci 2021 25;12:670497. Epub 2021 May 25.

Centro de Biotecnología y Genómica de Plantas, Instituto de Investigación y Tecnología Agraria y Alimentaria, Universidad Politécnica de Madrid, Madrid, Spain.

Perennial species in the boreal and temperate regions are subject to extreme annual variations in light and temperature. They precisely adapt to seasonal changes by synchronizing cycles of growth and dormancy with external cues. Annual dormancy-growth transitions and flowering involve factors that integrate environmental and endogenous signals. Read More

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GPR68 Contributes to Persistent Acidosis-Induced Activation of AGC Kinases and Tyrosine Phosphorylation in Organotypic Hippocampal Slices.

Front Neurosci 2021 25;15:692217. Epub 2021 May 25.

Department of Physiology and Cell Biology, University of South Alabama College of Medicine, Mobile, AL, United States.

Persistent acidosis occurs in ischemia and multiple neurological diseases. In previous studies, acidic stimulation leads to rapid increase in intracellular calcium in neurons. However, it remains largely unclear how a prolonged acidosis alters neuronal signaling. Read More

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Comprehensive Proteomic Profiling of Aqueous Humor Proteins in Proliferative Diabetic Retinopathy.

Transl Vis Sci Technol 2021 May;10(6)

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Purpose: Proliferative diabetic retinopathy (PDR) is a serious ocular disease that can lead to retinal microvascular complications in patients with diabetes mellitus. To date, no studies have explored PDR development by analyzing the aqueous humor (AH). Therefore we carried out tandem mass tag (TMT) proteomic quantification to compare AH protein profiles between PDR and non-PDR subjects. Read More

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Immuno-fibrotic drivers of impaired lung function in post-acute sequelae of SARS-CoV-2.

JCI Insight 2021 Jun 10. Epub 2021 Jun 10.

Department of Medicine, Duke University School of Medicine, Durham, United States of America.

Introduction: Subjects recovering from COVID-19 frequently experience persistent respiratory ailments which are key elements of post-acute sequelae of SARS-CoV-2 infection (PASC); however, little is known about the underlying biological factors that may direct lung recovery and the extent to which these are affected by COVID-19 severity.

Methods: We performed a prospective cohort study of subjects with persistent symptoms after acute COVID-19, collecting clinical data, pulmonary function tests, and plasma samples used for multiplex profiling of inflammatory, metabolic, angiogenic, and fibrotic factors.

Results: Sixty-one subjects were enrolled across two academic medical centers at a median of 9 weeks (interquartile range 6-10) after COVID-19 illness: n=13 subjects (21%) mild/non-hospitalized, n=30 (49%) hospitalized/non-critical, and n=18 subjects (30%) hospitalized/intensive care ("ICU"). Read More

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FOXR2 Stabilizes MYCN Protein and Identifies Non--Amplified Neuroblastoma Patients With Unfavorable Outcome.

J Clin Oncol 2021 Jun 10:JCO2002540. Epub 2021 Jun 10.

Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.

Purpose: Clinical outcomes of patients with neuroblastoma range from spontaneous tumor regression to fatality. Hence, understanding the mechanisms that cause tumor progression is crucial for the treatment of patients. In this study, we show that activation identifies a subset of neuroblastoma tumors with unfavorable outcome and we investigate the mechanism how FOXR2 relates to poor outcome in patients. Read More

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Proteomic analyses of plasma-derived exosomes in immunoglobulin (Ig) G4-related disease and their potential roles in B cell differentiation and tissue damage.

J Autoimmun 2021 Jun 6;122:102650. Epub 2021 Jun 6.

Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases, State Key Laboratory of Complex Severe and Rare Diseases, Beijing, China. Electronic address:

Objective: To investigate the proteomic profiles of plasma exosomes isolated from patients with immunoglobulin (Ig) G4-related disease (IgG4-RD) and to determine their potential roles in B cell differentiation and tissue damage.

Methods: One hundred untreated IgG4-RD patients and 135 sex- and age-matched healthy controls (HCs) were enrolled in this study. A combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and tandem mass tag (TMT)-label quantitation was used for proteomic profiling. Read More

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Immunomodulatory Effect of Serum Exosomes From Crohn Disease on Macrophages via Let-7b-5p/TLR4 Signaling.

Inflamm Bowel Dis 2021 Jun 9. Epub 2021 Jun 9.

Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, Hunan Province,  China.

Background: Exosomes are extensively reported to be strongly associated with many immunologic diseases, including Crohn disease (CD). Meanwhile, the dysfunction of macrophage activation has been proposed to be critical for the pathogenesis of CD. However, it is an unsettled issue whether serum exosomes from CD could activate macrophages and participate in its pathogenesis. Read More

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Transcriptional, hormonal, and metabolic changes in susceptible grape berries under powdery mildew infection.

J Exp Bot 2021 Jun 9. Epub 2021 Jun 9.

BioISI - Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Campo Grande, Lisboa, Portugal.

Vitis vinifera berries are extremely sensitive to infection by the biotrophic pathogen Erysiphe necator causing powdery mildew disease and deleterious effects on grape and wine quality. The combined analysis of the transcriptome and metabolome associated with this common fungal infection has not been previously carried out in any fruit. In order to identify the molecular, hormonal and metabolic mechanisms associated with infection, healthy and naturally infected Carignan berries were collected at two developmental stages: late green (EL33) and early véraison (EL35). Read More

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Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy.

Analyst 2021 Jun 9. Epub 2021 Jun 9.

Department of Biomedical Research and Radiology, University of Bern, Switzerland.

Background: Because of the interplay between mitochondrial respiration and cellular metabolism, the simultaneous monitoring of both cellular processes provides important insights for the understanding of biological processes. NMR flow systems provide a unique window into the metabolome of cultured cells. Simplified bioreactor construction based on commercially available flow systems increase the practicability and reproducibility of bioreactor studies using standard NMR spectrometers. Read More

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Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses.

J Vet Intern Med 2021 Jun 8. Epub 2021 Jun 8.

Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Hanover, Germany.

Background: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Read More

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Maternal RNF114-mediated target substrate degradation regulates zygotic genome activation in mouse embryos.

Development 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Suzhou Affiliated Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Nanjing 211166, China.

Zygotic genomic activation (ZGA) is a landmark event in the maternal-to-zygotic transition (MZT), and the regulation of ZGA by maternal factors remains to be elucidated. In this study, the depletion of maternal ring finger protein 114 (RNF114), a ubiquitin E3 ligase, led to 2-cell embryos developmental arrest in mice. RNF114 was proven to play a crucial role in major ZGA using immunofluorescence and transcriptome analysis. Read More

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CSF1R inhibition rescues tau pathology and neurodegeneration in an A/T/N model with combined AD pathologies, while preserving plaque associated microglia.

Acta Neuropathol Commun 2021 Jun 8;9(1):108. Epub 2021 Jun 8.

Department of Neurosciences, Biomedical Research Institute, Hasselt University, Hasselt, Belgium.

Alzheimer's disease (AD) is characterized by a sequential progression of amyloid plaques (A), neurofibrillary tangles (T) and neurodegeneration (N), constituting ATN pathology. While microglia are considered key contributors to AD pathogenesis, their contribution in the combined presence of ATN pathologies remains incompletely understood. As sensors of the brain microenvironment, microglial phenotypes and contributions are importantly defined by the pathologies in the brain, indicating the need for their analysis in preclinical models that recapitulate combined ATN pathologies, besides their role in A and T models only. Read More

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TNF blockade uncouples toxicity from antitumor efficacy induced with CD40 chemoimmunotherapy.

JCI Insight 2021 Jun 8. Epub 2021 Jun 8.

Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, United States of America.

Agonist CD40 antibodies are under clinical development in combination with chemotherapy as an approach to prime for anti-tumor T cell immunity. However, treatment with anti-CD40 is commonly accompanied by both systemic cytokine release and liver transaminase elevations which together account for the most common dose-limiting toxicities. Moreover, anti-CD40 treatment increases the potential for chemotherapy-induced hepatotoxicity. Read More

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Distinctive waves of innate immune response in the retina in experimental autoimmune encephalomyelitis.

JCI Insight 2021 Jun 8;6(11). Epub 2021 Jun 8.

Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, San Francisco, California, USA.

Neurodegeneration mediates neurological disability in inflammatory demyelinating diseases of the CNS. The role of innate immune cells in mediating this damage has remained controversial with evidence for destructive and protective effects. This has complicated efforts to develop treatment. Read More

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Neural cell adhesion molecule is required for ventricular conduction system development.

Development 2021 Jun 7;148(11). Epub 2021 Jun 7.

Leon H. Charney Division of Cardiology, Department of Medicine, NYU Grossman School of Medicine, NY 10016, USA.

The most distal portion of the ventricular conduction system (VCS) contains cardiac Purkinje cells (PCs), which are essential for synchronous activation of the ventricular myocardium. Contactin-2 (CNTN2), a member of the immunoglobulin superfamily of cell adhesion molecules (IgSF-CAMs), was previously identified as a marker of the VCS. Through differential transcriptional profiling, we discovered two additional highly enriched IgSF-CAMs in the VCS: NCAM-1 and ALCAM. Read More

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Insulin-like Growth Factor 1, but Not Insulin-Like Growth Factor-Binding Protein 3, Predicts Central Precocious Puberty in Girls 6-8 Years Old: A Retrospective Study.

Horm Res Paediatr 2021 Jun 7:1-8. Epub 2021 Jun 7.

Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.

Background: Central precocious puberty (CPP) in females is characterized by thelarche before 8 years of age. Evidence of reproductive axis activation confirms the diagnosis (basal serum luteinizing hormone (LH) ≥0.3 IU/L or LH-releasing hormone (LHRH)-stimulated LH ≥5 IU/L). Read More

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Identification of CDKL3 as a Critical Regulator in Development of Glioma through Regulating RRM2 and JNK signaling pathway.

Cancer Sci 2021 Jun 7. Epub 2021 Jun 7.

Department of Neurosurgery, Changzheng Hospital, Naval Medical University.

Glioma is one of the most commonly diagnosed intracranial malignancies. The molecular mechanism underlying the development of glioma is still largely unknown. In this study, we present the first report concerning the function and mechanism of CDKL3 in the development and prognosis of glioma. Read More

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Molecular and histopathological profiling of imiquimod induced dermatosis in Swiss Wistar rats: contribution to the rat model for novel anti-psoriasis treatments.

Mol Biol Rep 2021 Jun 7. Epub 2021 Jun 7.

Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Zmaja od Bosne 8, 71000, Sarajevo, Bosnia and Herzegovina.

Imiquimod (IMQ) induced human-like psoriasis in mice has been shown to be effective in testing and development of novel treatments. The IMQ psoriasis model has become widely used animal model, however, it is not completely characterized in different rat strains. We aimed to evaluate IMQ and betamethasone treatment for induction and reversal of psoriatic lesions on macroscopic, histological, genetic as well as cytokines and chemokines activation levels. Read More

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Vaccine-induced ICOSCD38 circulating Tfh are sensitive biosensors of age-related changes in inflammatory pathways.

Cell Rep Med 2021 May 7;2(5):100262. Epub 2021 May 7.

Institute for Immunology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.

Humoral immune responses are dysregulated with aging, but the cellular and molecular pathways involved remain incompletely understood. In particular, little is known about the effects of aging on T follicular helper (Tfh) CD4 cells, the key cells that provide help to B cells for effective humoral immunity. We performed transcriptional profiling and cellular analysis on circulating Tfh before and after influenza vaccination in young and elderly adults. Read More

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Effect of Voacamine upon inhibition of hypoxia induced fatty acid synthesis in a rat model of methyln-nitrosourea induced mammary gland carcinoma.

BMC Mol Cell Biol 2021 Jun 5;22(1):33. Epub 2021 Jun 5.

Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, VidyaVihar, Raebareily Road, Lucknow, 226025, India.

Background: In the present study, fatty acid synthesis is targeted to combat mammary gland carcinoma by activating prolyl hydroxylase-2 with Voacamine alone and in combination with Tamoxifen. It was hypothesized that the activation of prolyl hydroxylase-2 would inhibit the hypoxia-induced fatty acid synthesis and mammary gland carcinoma. Mammary gland carcinoma was induced with a single dose administration of N-methyl-N-nitrosourea (50 mg/kg,i. Read More

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Inhibition of EZH2 primes the cardiac gene activation via removal of epigenetic repression during human direct cardiac reprogramming.

Stem Cell Res 2021 May 27;53:102365. Epub 2021 Apr 27.

Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35233, USA. Electronic address:

Cardiovascular disease, until now, is still the leading cause of death in the United States. Due to the limited regenerative capacity of adult hearts, the damage caused by heart injury cannot be reversed and eventually progress into heart failure. In need of cardiovascular disease treatment, many therapies aimed at either cell transplantation or cell regeneration have been proposed. Read More

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Nanolu0ciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction.

Methods Mol Biol 2021 ;2268:149-157

Laboratory of Molecular Pharmacology, GIGA-Molecular Biology of Diseases, University of Liège, Liège, Belgium.

G protein-coupled receptors (GPCR) are one of the principal class of membrane proteins and around 30% of the currently marketed drugs act on one of them. The efficacious detection of ligands with the desired pharmacological profile remains a challenge of paramount importance in the GPCR drug discovery and pharmacological research. Recent evidences demonstrate that GPCR ligands can stabilize distinct receptor conformation and trigger various signaling pathways with different efficacies and/or potencies. Read More

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January 2021

Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines.

Commun Biol 2021 Jun 3;4(1):677. Epub 2021 Jun 3.

Division of Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, Lund, Sweden.

Immortalized erythroid cell lines are expected to be a promising source of ex vivo manufactured red blood cells (RBCs), however the induction of enucleation in these cell lines is inefficient at present. We utilized an imaging-based high-throughput system to identify chemical compounds that trigger enucleation of human erythroid cell lines. Among >3,300 compounds, we identified multiple histone deacetylase inhibitors (HDACi) inducing enucleated cells from the cell line, although an increase in membrane fragility of enucleated cells was observed. Read More

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Post-diapause transcriptomic restarts: insight from a high-latitude copepod.

BMC Genomics 2021 Jun 3;22(1):409. Epub 2021 Jun 3.

Pacific Biosciences Research Center, University of Hawai'i at Mānoa, 1993 East-West Rd, Honolulu, HI, 96822, USA.

Background: Diapause is a seasonal dormancy that allows organisms to survive unfavorable conditions and optimizes the timing of reproduction and growth. Emergence from diapause reverses the state of arrested development and metabolic suppression returning the organism to an active state. The physiological mechanisms that regulate the transition from diapause to post-diapause are still unknown. Read More

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Calcium signaling mediates a biphasic mechanoadaptive response of endothelial cells to cyclic mechanical stretch.

Mol Biol Cell 2021 Jun 3:mbcE21030106. Epub 2021 Jun 3.

Université Grenoble Alpes, Institute for Advanced Biosciences, Grenoble 38042, France.

The vascular system is precisely regulated to adjust blood flow to organismal demand, thereby guaranteeing adequate perfusion under varying physiological conditions. Mechanical forces, such as cyclic circumferential stretch, are among the critical stimuli that dynamically adjust vessel distribution and diameter, but the precise mechanisms of adaptation to changing forces are unclear. We find that endothelial monolayers respond to cyclic stretch by transient remodeling of the VE-Cadherin-based adherens junctions and the associated actomyosin cytoskeleton. Read More

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Recent Advances in Single-Cell Profiling and Multispecific Therapeutics: Paving the Way for a New Era of Precision Medicine Targeting Cardiac Fibroblasts.

Curr Cardiol Rep 2021 Jun 3;23(7):82. Epub 2021 Jun 3.

Department of Cardiometabolic Disorders, Amgen Discovery Research, Amgen Inc., 1120 Veterans Blvd, South San Francisco, CA, 94080, USA.

Purpose Of Review: Cardiac fibroblast activation contributes to fibrosis, maladaptive remodeling and heart failure progression. This review summarizes the latest findings on cardiac fibroblast activation dynamics derived from single-cell transcriptomic analyses and discusses how this information may aid the development of new multispecific medicines.

Recent Findings: Advances in single-cell gene expression technologies have led to the discovery of distinct fibroblast subsets, some of which are more prevalent in diseased tissue and exhibit temporal changes in response to injury. Read More

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