30 results match your criteria profiles procyclic

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Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi.

J Proteomics 2020 09 14;227:103902. Epub 2020 Jul 14.

Laboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. Electronic address:

Leishmania infantum chagasi is the primary etiological agent of visceral leishmaniasis in Latin America, a lethal disease that afflicts hundreds of thousands of people worldwide every year. Previous studies have shown that the parasite releases microvesicles known as exosomes, which prolong and exacerbate infection in the vertebrate vector. However, little is known of their role in the insect vector, the sand fly Lutzomyia longipalpis. Read More

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September 2020

Effect of lysine acetylation on the regulation of Trypanosoma brucei glycosomal aldolase activity.

Biochem J 2020 05;477(9):1733-1744

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.

Post-translational modifications provide suitable mechanisms for cellular adaptation to environmental changes. Lysine acetylation is one of these modifications and occurs with the addition of an acetyl group to Nε-amino chain of this residue, eliminating its positive charge. Recently, we found distinct acetylation profiles of procyclic and bloodstream forms of Trypanosoma brucei, the agent of African Trypanosomiasis. Read More

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Proteome turnover in the bloodstream and procyclic forms of measured by quantitative proteomics.

Wellcome Open Res 2019 9;4:152. Epub 2019 Oct 9.

The Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, UK.

: Cellular proteins vary significantly in both abundance and turnover rates. These parameters depend upon their rates of synthesis and degradation and it is useful to have access to data on protein turnover rates when, for example, designing genetic knock-down experiments or assessing the potential usefulness of covalent enzyme inhibitors. Little is known about the nature and regulation of protein turnover in , the etiological agent of human and animal African trypanosomiasis. Read More

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October 2019

A quantitative proteomic and bioinformatics analysis of proteins in metacyclogenesis of Leishmania tropica.

Acta Trop 2020 Feb 21;202:105227. Epub 2019 Oct 21.

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Recently there has a growing interest in MS-based analysis on Leishmania for biology study, host-parasite interaction and drug target discovery. The aims of this study were to analyzed protein profiles in the procyclic and metacyclic stages of L. tropica, and investigate their potential role in metacyclogenesis molecular mechanisms. Read More

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February 2020

Axenic amastigote cultivation and in vitro development of Leishmania orientalis.

Parasitol Res 2019 Jun 10;118(6):1885-1897. Epub 2019 Apr 10.

Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK.

Leishmania (Mundinia) orientalis is a recently described new species that causes leishmaniasis in Thailand. To facilitate characterization of this new species, an in vitro culture system to generate L. orientalis axenic amastigotes was developed. Read More

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RNA-Seq analysis validates the use of culture-derived Trypanosoma brucei and provides new markers for mammalian and insect life-cycle stages.

BMC Genomics 2018 Apr 2;19(1):227. Epub 2018 Apr 2.

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH-3012, Bern, Switzerland.

Background: Trypanosoma brucei brucei, the parasite causing Nagana in domestic animals, is closely related to the parasites causing sleeping sickness, but does not infect humans. In addition to its importance as a pathogen, the relative ease of genetic manipulation and an innate capacity for RNAi extend its use as a model organism in cell and infection biology. During its development in its mammalian and insect (tsetse fly) hosts, T. Read More

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Cell cycle synchronisation of Trypanosoma brucei by centrifugal counter-flow elutriation reveals the timing of nuclear and kinetoplast DNA replication.

Sci Rep 2017 12 14;7(1):17599. Epub 2017 Dec 14.

Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK.

We report an optimised centrifugal counter-flow elutriation protocol for the rapid and direct isolation of G1 cell cycle synchronised populations of both the procyclic and bloodstream form stages of Trypanosoma brucei that yields viable and proliferative cells. The high quality of the synchronisation achieved can be judged by the uniform DNA content, narrow size distribution, synchronous division, and the maintenance of synchronicity into subsequent cell cycles. We show that early-eluting fractions represent different G1 subpopulations that progress through the cell cycle with distinct temporal profiles post-elutriation, as exemplified by the observation of the maturation of a second flagellar basal body in late G1 phase, DNA replication in S phase, and dimethylation of histone H3 in mitosis/cytokinesis. Read More

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December 2017

Comparative Proteomic Analysis of Lysine Acetylation in Trypanosomes.

J Proteome Res 2018 01 11;17(1):374-385. Epub 2017 Dec 11.

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo , R. Pedro de Toledo 669 L6A, 04039-032 São Paulo, SP, Brazil.

Protein acetylation is a post-translational modification regulating diverse cellular processes. By using proteomic approaches, we identified N-terminal and ε-lysine acetylated proteins in Trypanosoma cruzi and Trypanosoma brucei, which are protozoan parasites that cause significant human and animal diseases. We detected 288 lysine acetylation sites in 210 proteins of procyclic form, an insect stage of T. Read More

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January 2018

TbIRK is a signature sequence free potassium channel from Trypanosoma brucei locating to acidocalcisomes.

Sci Rep 2017 04 6;7(1):656. Epub 2017 Apr 6.

Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

Potassium channels from prokaryotes and eukaryotes are usually recognized by a typical amino acid sequence TXTGY(F)G representing the ionic selectivity filter. Using a screening approach with ion channel family profiles but without the above motif, we identified a gene in Trypanosoma brucei that exhibits homology to inward rectifying potassium channels. We report here cloning of this ion channel named TbIRK. Read More

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The Transcriptome of Developmental Stages in Their Natural Sand Fly Vector.

mBio 2017 04 4;8(2). Epub 2017 Apr 4.

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

The life cycle of the parasite in the sand fly vector involves differentiation into several distinctive forms, each thought to represent an adaptation to specific microenvironments in the midgut of the fly. Based on transcriptome sequencing (RNA-Seq) results, we describe the first high-resolution analysis of the transcriptome dynamics of four distinct stages of as they develop in a natural vector, The early transformation from tissue amastigotes to procyclic promastigotes in the blood-fed midgut was accompanied by the greatest number of differentially expressed genes, including the downregulation of amastins, and upregulation of multiple cell surface proteins, sugar and amino acid transporters, and genes related to glucose metabolism and cell cycle progression. The global changes accompanying post-blood meal differentiation of procyclic promastigotes to the nectomonad and metacyclic stages were less extensive, though each displayed a unique signature. Read More

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24-Methylenecyclopropane steroidal inhibitors: A Trojan horse in ergosterol biosynthesis that prevents growth of Trypanosoma brucei.

Biochim Biophys Acta Mol Cell Biol Lipids 2017 Mar 6;1862(3):305-313. Epub 2016 Dec 6.

Department of Chemistry and Biochemistry and Center for Chemical Biology, Texas Tech University, Lubbock, TX 79409, USA. Electronic address:

A new class of steroidal therapeutics based on phylogenetic-guided design of covalent inhibitors that target parasite-specific enzymes of ergosterol biosynthesis is shown to prevent growth of the protozoan-Trypanosoma brucei, responsible for sleeping sickness. In the presence of approximately 15±5μM 26,27-dehydrolanosterol, T. brucei procyclic or blood stream form growth is inhibited by 50%. Read More

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T7 polymerase-driven transcription is downregulated in metacyclic promastigotes and amastigotes of Leishmania mexicana.

Folia Parasitol (Praha) 2016 May 18;63. Epub 2016 May 18.

Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.

In our previous work we established a T7 polymerase-driven Tetracycline-inducible protein expression system in Leishmania mexicana (Biagi, 1953). We used this system to analyse gene expression profiles during development of L. mexicana in procyclic and metacyclic promastigotes and amastigotes. Read More

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Biosynthesis of very long chain fatty acids in Trypanosoma cruzi.

Parasitol Res 2015 Jan 24;114(1):265-71. Epub 2014 Oct 24.

Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Ocampo y Esmeralda, 2000, Rosario, Santa Fe, Argentina.

Trypanosoma brucei and Trypanosoma cruzi showed similar fatty acid (FA) compositions, having a high proportion of unsaturated FAs, mainly 18:2Δ9,12 (23-39%) and 18:1Δ9 (11-17%). C22 polyunsaturated FAs are in significant amounts only in T. brucei (12-20%) but represent a mere 2% of total FAs in T. Read More

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January 2015

Functional interplay between protein arginine methyltransferases in Trypanosoma brucei.

Microbiologyopen 2014 Oct 7;3(5):595-609. Epub 2014 Jul 7.

Department of Microbiology and Immunology, University at Buffalo School of Medicine, Buffalo, New York, 14214.

Arginine methylation is a common posttranslational modification that has far-reaching cellular effects. Trypanosoma brucei is an early-branching eukaryote with four characterized protein arginine methyltransferases (PRMTs), one additional putative PRMT, and over 800 arginine methylated proteins, suggesting that arginine methylation has widespread impacts in this organism. While much is known about the activities of individual T. Read More

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October 2014

Ribosome biogenesis requires a highly diverged XRN family 5'->3' exoribonuclease for rRNA processing in Trypanosoma brucei.

RNA 2013 Oct 23;19(10):1419-31. Epub 2013 Aug 23.

Although biogenesis of ribosomes is a crucial process in all organisms and is thus well conserved, Trypanosoma brucei ribosome biogenesis, of which maturation of rRNAs is an early step, has multiple points of divergence. Our aim was to determine whether in the processing of the pre-rRNA precursor molecule, 5'→3' exoribonuclease activity in addition to endonucleolytic cleavage is necessary in T. brucei as in other organisms. Read More

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October 2013

A minimal anaphase promoting complex/cyclosome (APC/C) in Trypanosoma brucei.

PLoS One 2013 22;8(3):e59258. Epub 2013 Mar 22.

Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America.

The anaphase-promoting complex/cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase that initiates chromosome segregation and mitotic exit by targeting critical cell-cycle regulators for proteolytic destruction. Previously, seven APC/C subunit homologues were identified in the genome of Trypanosoma brucei. In the present study, we tested five of them in yeast complementation studies and found none of them capable of complementing the yeast mutants lacking the corresponding subunits, suggesting significant discrepancies between the two APC/C's. Read More

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September 2013

Novel sterol metabolic network of Trypanosoma brucei procyclic and bloodstream forms.

Biochem J 2012 Apr;443(1):267-77

Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.

Trypanosoma brucei is the protozoan parasite that causes African trypanosomiasis, a neglected disease of people and animals. Co-metabolite analysis, labelling studies using [methyl-2H3]-methionine and substrate/product specificities of the cloned 24-SMT (sterol C24-methyltransferase) and 14-SDM (sterol C14demethylase) from T. brucei afforded an uncommon sterol metabolic network that proceeds from lanosterol and 31-norlanosterol to ETO [ergosta-5,7,25(27)-trien-3β-ol], 24-DTO [dimethyl ergosta-5,7,25(27)-trienol] and ergosterol [ergosta-5,7,22(23)-trienol]. Read More

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Identification of subspecies specific genes differentially expressed in procyclic forms of Trypanosoma brucei subspecies.

Infect Genet Evol 2010 Mar 11;10(2):229-37. Epub 2009 Nov 11.

Deutsches Krebsforschungszentrum, Division of Functional Genome Analysis (B070), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.

Trypanosoma brucei subspecies undergo establishment and maturation in tsetse flies mid-gut and salivary glands, respectively. Successful establishment of trypanosomes in tsetse mid-gut as well as their migration to saliva gland depends on the ability of these parasites to adapt rapidly to new environmental conditions and to negotiate the physical barriers. To identify subspecies specific genes which are differentially regulated during the establishment of T. Read More

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Genome-wide expression profiling of in vivo-derived bloodstream parasite stages and dynamic analysis of mRNA alterations during synchronous differentiation in Trypanosoma brucei.

BMC Genomics 2009 Sep 11;10:427. Epub 2009 Sep 11.

Centre for Immunity, Infection and Evolution, Institute of Immunology and Infection Research, School of Biological Sciences, King's Buildings, University of Edinburgh, Edinburgh, UK.

Background: Trypanosomes undergo extensive developmental changes during their complex life cycle. Crucial among these is the transition between slender and stumpy bloodstream forms and, thereafter, the differentiation from stumpy to tsetse-midgut procyclic forms. These developmental events are highly regulated, temporally reproducible and accompanied by expression changes mediated almost exclusively at the post-transcriptional level. Read More

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September 2009

Genome-wide analysis reveals increased levels of transcripts related with infectivity in peanut lectin non-agglutinated promastigotes of Leishmania infantum.

Genomics 2009 Jun 10;93(6):551-64. Epub 2009 Feb 10.

Departamento de Ciencia de Proteínas and Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Calle Ramiro de Maeztu 9, 28040 Madrid, Spain.

Metacyclic promastigotes are transmitted during bloodmeals after development inside the gut of the sandfly vector. The isolation from axenic cultures of procyclic and metacyclic promastigotes by peanut lectin agglutination followed by differential centrifugation is controversial in Leishmania infantum. The purpose of this study has been to isolate both fractions simultaneously from the same population in stationary phase of axenic culture and compare their expression profiles by whole-genome shotgun DNA microarrays. Read More

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The LAMP-like protein p67 plays an essential role in the lysosome of African trypanosomes.

Mol Microbiol 2008 May;68(4):933-46

Department of Medical Microbiology & Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.

RNAi knockdown was employed to study the function of p67, a lysosome-associated membrane protein (LAMP)-like type I transmembrane lysosomal glycoprotein in African trypanosomes. Conditional induction of p67 dsRNA resulted in specific approximately 90% reductions in de novo p67 synthesis in both mammalian bloodstream and procyclic insect-stage parasites. Bloodstream cell growth was severely retarded with extensive death after > 24 h of induction. Read More

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Telomere length regulation and transcriptional silencing in KU80-deficient Trypanosoma brucei.

Nucleic Acids Res 2004 15;32(22):6575-84. Epub 2004 Dec 15.

Laboratory of Molecular Parasitology, The Rockefeller University, Box 185, 1230 York Avenue, New York, NY 10021-6399, USA.

KU is a heterodimer, consisting of approximately 70 and approximately 80 kDa subunits (KU70 and KU80, respectively), which is involved in a variety of nuclear functions. We generated tbKU80-deficient trypanosomes to explore the potential role of the tbKU complex in telomere maintenance and transcriptional regulation of variant surface glycoprotein (VSG) genes in Trypanosoma brucei. Using real-time PCR, we demonstrated that the expression of several different VSG genes remains tightly regulated in tbKU80-deficient bloodstream-form cell lines, suggesting that VSG transcription profiles do not change in these cells. Read More

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January 2005

Proteomic analysis of Leishmania mexicana differentiation.

Mol Biochem Parasitol 2004 Jul;136(1):51-62

Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College London, Exhibition Road, London SW7 2AZ, UK.

We have resolved the proteome of axenically differentiated Leishmania mexicana parasites by two-dimensional gel electrophoresis (2DE), employing optimised, robust and reproducible procedures, and visualised (by silver staining) approximately 2000 protein species in each of three developmental stages: procyclic promastigotes, metacyclic promastigotes and amastigotes. This analysis has used homogeneous populations of these parasite stages, characterised according to their morphology, protease and nuclease activity profiles and expression of stage-specific antigens. Following comparison of the whole proteome profiles between stages, 47 spots were found to be stage-specific, while a further 100 spots changed in intensity during differentiation. Read More

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Changes in polysome profiles accompany trypanosome development.

Authors:
M Brecht M Parsons

Mol Biochem Parasitol 1998 Nov;97(1-2):189-98

Seattle Biomedical Research Institute, WA 98109, USA.

Development of the protozoan pathogen Trypanosoma brucei involves regulated changes in parasite structure, biochemistry, and the cell cycle. The transition of slender blood forms into stumpy bloodforms includes cell cycle arrest and a decrease in protein synthesis. The next stage in the development cycle, the procyclic form, shows increased protein synthesis and proliferates. Read More

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November 1998

Differentiation of a culture-adapted mutant bloodstream form of Trypanosoma brucei into the procyclic form results in growth arrest of the cells.

Mol Biochem Parasitol 1995 Jun;72(1-2):215-25

Department of Pharmaceutical Chemistry, University of California San Francisco 94143-0446, USA.

The bloodstream forms of Trypanosoma brucei monomorphic strain 427 serially passaged in rats can differentiate in vitro equally well in HMI-9, HMI-10, SDM-79 or Cunningham's medium into the insect (procyclic) forms by a simple temperature shift from 37 to 26 degrees C in the presence of citrate and cis-aconitate. The procyclic forms thus generated can also continue to multiply at 26 degrees C without replacing the culture medium. The same strain of T. Read More

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Trypanosoma congolense: developmental regulation of protein kinases and tyrosine phosphorylation during the life cycle.

Exp Parasitol 1995 May;80(3):507-14

Seattle Biomedical Research Institute, Washington 98109, USA.

In higher eukaryotes, key steps in the control of growth and proliferation are regulated by protein phosphorylation. However, little is known about the role of protein phosphorylation in the developmental cycles of pathogenic protozoa. In Trypanosoma brucei, only the bloodform and procyclic form stages can be obtained in sufficient numbers for biochemical analyses. Read More

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Characterization of concanavalin A-binding glycoproteins from procyclic culture forms of Trypanosoma congolense, T. simiae and T. brucei brucei.

Parasitol Res 1995 ;81(3):245-52

Department of Microbiology, College of Biological Sciences, University of Guelph, Ont., Canada.

Concanavalin A-binding glycoproteins were obtained from procyclic culture forms (PCFs) of Trypanosoma congolense, T. simiae, and T. b. Read More

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Trypanosoma brucei brucei and Trypanosoma brucei gambiense: stage specific differences in wheat germ agglutinin binding and in endoglycosidase H sensitivity of glycoprotein oligosaccharides.

Exp Parasitol 1987 Aug;64(1):104-10

Gel electrophoresis, lectin affinity blotting, and endoglycosidase H digestion have been used to analyze the glycoprotein profiles of bloodstream and procyclic forms of Trypanosoma brucei brucei and T. b. gambiense. Read More

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Thiol-dependent proteases of African trypanosomes. Analysis by electrophoresis in sodium dodecyl sulphate/polyacrylamide gels co-polymerized with fibrinogen.

Eur J Biochem 1986 Mar;155(3):469-73

The proteases of several species of African trypanosomes were analysed by electrophoresis in sodium dodecyl sulphate/polyacrylamide gels containing fibrinogen or collagen. After electrophoresis the gels were incubated in the presence of enzyme activators and/or inhibitors and then stained with Coomassie brilliant blue. The areas where the proteolytic activity had degraded the fibrinogen did not stain and so formed clear bands against a blue background. Read More

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Kinetoplast DNA and RNA of Trypanosoma brucei.

Mol Biochem Parasitol 1980 Dec;2(2):93-108

Kinetoplast DNA (kDNA) and kinetoplast RNA (kRNA) were isolated from bloodstream and procyclic culture forms of two clonal strains of Trypanosoma brucei. No differences were observed in kDNA (maxicircle) restriction profiles between bloodstream or procyclic culture forms of the same strain. Some differences were observed in kDNA maxicircle restriction sites between the two strains. Read More

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December 1980
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