54,413 results match your criteria profiles potential

Screening and identification of autophagy-related biomarkers for oral squamous cell carcinoma (OSCC) via integrated bioinformatics analysis.

J Cell Mol Med 2021 Apr 9. Epub 2021 Apr 9.

Department of Oral & Maxillofacial Surgery, NanFang Hospital, Southern Medical University, Guangzhou, China.

Increasing evidences have showed that autophagy played a significant role in oral squamous cell carcinoma (OSCC). Purpose of our study was to explore the prognostic value of autophagy-related genes (ATGs) and screen autophagy-related biomarkers for OSCC. RNA-seq and clinical data were downloaded from The Cancer Genome Atlas (TCGA) database following extracting ATG expression profiles. Read More

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Contrasting Composition, Diversity and Predictive Metabolic Potential of the Rhizobacterial Microbiomes Associated with Native and Invasive Prosopis Congeners.

Curr Microbiol 2021 Apr 10. Epub 2021 Apr 10.

National Centre for Cell Sciences, Pune University Campus, Ganeskhind, Pune, 411007, India.

Invasive plants are known to alter the soil microbial communities; however, the effects of co-occurring native and invasive congeners on the soil bacterial diversity and their predictive metabolic profiles are not known. Here, we compared the rhizosphere bacterial communities of invasive Prosopis juliflora and its native congener Prosopis cineraria using high-throughput sequencing of the 16S rRNA gene. Unweighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram revealed significant variation in the communities of these co-occurring Prosopis species. Read More

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A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation.

Nat Commun 2021 Apr 9;12(1):2126. Epub 2021 Apr 9.

Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.

There is accumulating evidence that the lower airway microbiota impacts lung health. However, the link between microbial community composition and lung homeostasis remains elusive. We combine amplicon sequencing and bacterial culturing to characterize the viable bacterial community in 234 longitudinal bronchoalveolar lavage samples from 64 lung transplant recipients and establish links to viral loads, host gene expression, lung function, and transplant health. Read More

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Rhizosphere community selection reveals bacteria associated with reduced root disease.

Microbiome 2021 Apr 9;9(1):86. Epub 2021 Apr 9.

USDA-ARS, Wheat Health, Genetics and Quality Research Unit, Washington State University, Pullman, WA, 99164-6430, USA.

Background: Microbes benefit plants by increasing nutrient availability, producing plant growth hormones, and protecting against pathogens. However, it is largely unknown how plants change root microbial communities.

Results: In this study, we used a multi-cycle selection system and infection by the soilborne fungal pathogen Rhizoctonia solani AG8 (hereafter AG8) to examine how plants impact the rhizosphere bacterial community and recruit beneficial microorganisms to suppress soilborne fungal pathogens and promote plant growth. Read More

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LINC00221 silencing prevents the progression of hepatocellular carcinoma through let-7a-5p-targeted inhibition of MMP11.

Cancer Cell Int 2021 Apr 9;21(1):202. Epub 2021 Apr 9.

Department of Oncology, Shaanxi Province, Affiliated Hospital of the Shaanxi University of Traditional Chinese Medicine, No. 2, Weiyang West Road, Xianyang, 712000, People's Republic of China.

Background: Microarray profiles of hepatocellular carcinoma (HCC) identified that long intergenic noncoding RNA 00221 (LINC00221) was upregulated. Herein, we aimed to identify the functional significance and underlying mechanisms of LINC00221 in HCC.

Methods And Results: Human HCC samples had increased expression of LINC00221. Read More

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Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux.

BMC Oral Health 2021 Apr 9;21(1):183. Epub 2021 Apr 9.

Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zurich, Switzerland.

Background: This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear.

Methods: Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1-5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0. Read More

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Identification of COVID-19 subtypes based on immunogenomic profiling.

Int Immunopharmacol 2021 Mar 27;96:107615. Epub 2021 Mar 27.

Biomedical Informatics Research Lab, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Big Data Research Institute, China Pharmaceutical University, Nanjing 211198, China. Electronic address:

Although previous studies have shown that the host immune response is crucial in determining clinical outcomes in COVID-19 patients, the association between host immune signatures and COVID-19 patient outcomes remains unclear. Based on the enrichment levels of 11 immune signatures (eight immune-inciting and three immune-inhibiting signatures) in leukocytes of 100 COVID-19 patients, we identified three COVID-19 subtypes: Im-C1, Im-C2, and Im-C3, by clustering analysis. Im-C1 had the lowest immune-inciting signatures and high immune-inhibiting signatures. Read More

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Multi-element signature of cuttlefish and its potential for the discrimination of different geographical provenances and traceability.

Food Chem 2021 Mar 26;356:129687. Epub 2021 Mar 26.

Department of Food and Drug, University of Parma, Parma, Via del Taglio, 10, Parma 43126, Italy. Electronic address:

The measurement and analysis of fifty-two elements by quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS) and direct mercury analysis were applied to origin discrimination of Italian traditional cuttlefish (Chioggia, Venice lagoon) from Mediterranean and Atlantic samples. A total 68 specimens were analyzed in triplicates to generate 204 mass spectra profiles which were statistically processed by different chemometric techniques. Loading weights from principal component analysis as input for linear discriminant analysis (LW-LDA), stepwise-LDA (S-LDA) and variable influence of projection-partial least square discriminant analysis (VIP-PLS-DA) were used to classify samples while retaining the lowest possible number of key variables. Read More

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Discovery of emerging sulfur-containing PAHs in PM: Contamination profiles and potential health risks.

J Hazard Mater 2021 Apr 1;416:125795. Epub 2021 Apr 1.

State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China; Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China. Electronic address:

We reported the discovery and identification of emerging sulfur-containing polycyclic aromatic hydrocarbons, namely polycyclic aromatic sulfur heterocycles (PASHs), in PM collected from two typical regions of China, Taiyuan and Guangzhou. Until now, there is no research on contamination status, sources and potential health risks of this unexpected group of organic contaminants in PM. High atmospheric concentrations (ngm) and significant time-dependent variations were determined in PM of Taiyuan from 2017 to 2018. Read More

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Synthesis and anti-inflammatory activity of novel steroidal chalcones with 3β-pregnenolone ester derivatives in RAW 264.7 cells in vitro.

Steroids 2021 Apr 6:108830. Epub 2021 Apr 6.

Department of Pharmacology, Shantou University Medical College, Shantou 515041, Guangdong, China. Electronic address:

To identify new potential anti-inflammatory agents, we herein report the synthesis of novel steroidal chalcones with 3β-pregnenolone esters of cinnamic acid derivatives using pregnenolone as the starting material. The structures of the newly synthesised compounds were confirmed by H-NMR, C-NMR, HRMS and infrared imaging. All the derivatives were examined to determine their in vitro anti-inflammatory profiles against LPS-induced inflammation in RAW 264. Read More

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Determining the Utility of the Guillain-Barré Syndrome Classification Criteria.

J Clin Neurol 2021 Apr;17(2):273-282

Division of Neurology, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Background And Purpose: Several variants of Guillain-Barré syndrome (GBS) and Miller Fisher syndrome (MFS) exist, but their frequencies vary in different populations and do not always meet the inclusion criteria of the existing diagnostic criteria. However, the GBS classification criteria by Wakerley and colleagues recognize and define the clinical characteristics of each variant. We applied these criteria to a GBS and MFS cohort with the aim of determining their utility. Read More

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Establishment of a post-race biomarkers database and application of pathway analysis to identify potential biomarkers in post-race equine plasma.

Drug Test Anal 2021 Apr 9. Epub 2021 Apr 9.

Drug Analysis Department, Laboratory of Racing Chemistry, 1731-2, Tsuruta-machi, Utsunomiya, Tochigi, 320-0851, Japan.

In the context of doping control, conventional direct chemical testing detects only a limited scope of target substances in equine biological samples. To expand the ability to detect doping agents and their detection windows, metabolomics has recently become a common approach for monitoring alteration of biomarkers caused by doping agents in relevant metabolic pathways. In horse racing, remarkable changes in metabolic profiles between the rest state and racing are likely to affect the identification of doping biomarkers. Read More

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Single-Cell RNA Sequencing Analysis: A Step-by-Step Overview.

Methods Mol Biol 2021 ;2284:343-365

Telethon Institute of Genetics and Medicine (TIGEM), Armenise/Harvard Laboratory of Integrative Genomics, Pozzuoli, Italy.

Thanks to innovative sample-preparation and sequencing technologies, gene expression in individual cells can now be measured for thousands of cells in a single experiment. Since its introduction, single-cell RNA sequencing (scRNA-seq) approaches have revolutionized the genomics field as they created unprecedented opportunities for resolving cell heterogeneity by exploring gene expression profiles at a single-cell resolution. However, the rapidly evolving field of scRNA-seq invoked the emergence of various analytics approaches aimed to maximize the full potential of this novel strategy. Read More

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January 2021

Update on the Impact of Comorbidities on the Efficacy and Safety of Heart Failure Medications.

Curr Heart Fail Rep 2021 Apr 9. Epub 2021 Apr 9.

Division of Cardiology, Duke University School of Medicine, Durham, NC, USA.

Purpose Of Review: Multiple newer medications benefit patients with heart failure with reduced ejection fraction (HFrEF). While these therapies benefit the broad population with HFrEF, the efficacy and safety of these therapies have been less well characterized in patients with significant comorbidities.

Recent Findings: Common comorbidities of high interest in heart failure (HF) include diabetes mellitus, chronic kidney disease (CKD), atrial fibrillation, and obesity, and each has potential implications for clinical management. Read More

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Profiles of inflammation factors and inflammatory pathways around the peri-miniscrew implant.

Histol Histopathol 2021 Apr 9:18336. Epub 2021 Apr 9.

Department of Orthodontics, Stomatological Hospital, Southern Medical University, Guangzhou, China.

Background: Peri-miniscrew implant is a temporary assistant armamentarium for the treatment of severe malocclusion and complex tooth movement, the inflammation around it is the main reason for the failure of orthodontic treatment due to the implant loosening and falling out. Inflammation around the peri-miniscrew implant is associated with the release of pro-inflammatory cytokines. These pro-inflammatory cytokines, in turn, recruit immune cells (such as macrophages, dendritic cells, T cells, and B cells), which can produce and release inflammatory biomarkers, regulate the interaction between immune cells, periodontal ligament cells, osteoblasts, and so on. Read More

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Circulating miR-320a Acts as a Tumor Suppressor and Prognostic Factor in Non-small Cell Lung Cancer.

Front Oncol 2021 23;11:645475. Epub 2021 Mar 23.

Department of Zoology, Central University of Punjab, Bathinda, India.

Dysregulated expression profiles of microRNAs (miRNAs) have been observed in several types of cancer, including non-small cell lung cancer (NSCLC); however, the diagnostic and prognostic potential of circulating miRNAs in NSCLC remains largely undefined. Here we found that circulating miR-320a was significantly down-regulated (~5.87-fold; < 0. Read More

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Analysis of the PD-1 Ligands Among Gastrointestinal Cancer Patients: Focus on Cancer Immunity.

Front Oncol 2021 23;11:637015. Epub 2021 Mar 23.

Department of Medical Imaging and Interventional Radiology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Many types of gastrointestinal cancer have shown promising outcomes after checkpoint blockade immunotherapy; however, it remains largely unclear about the expression profiles of programmed death 1 (PD-1) ligands (CD274 and PDCD1LG2) in the context of human pan-cancer. This work comprehensively analyzed the expression pattern of the PD-1 ligands and the clinical significance in the prognosis prediction among the seven types of gastrointestinal malignancies collected from The Cancer Genome Atlas (TCGA) and the Cancer Cell Line Encyclopedia (CCLE) database. Furthermore, the correlation of CD274/PDCD1LG2 with cancer immunity was also explored. Read More

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Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients.

J Nutr Metab 2021 26;2021:5527736. Epub 2021 Mar 26.

Department of Internal Medicine, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, Indonesia.

Background: Altered lipid profiles have consistently been linked to cerebrovascular events. Ischemic stroke (IS) was a common comorbid condition established in type 2 diabetes mellitus (T2DM). The apolipoprotein E (ApoE) gene which has a notably critical function in lipoprotein metabolism is believed as one of the potential candidate genes susceptible to IS complications in T2DM. Read More

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Integrated Analysis of ceRNA Regulatory Network Associated With Tumor Stage in Cervical Cancer.

Front Genet 2021 23;12:618753. Epub 2021 Mar 23.

Department of Oncology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.

Objective: To analyze the abnormally expressed genes involved in cervical cancer occurrence and development.

Materials And Methods: Integrated bioinformatics methods were used to analyze differentially expressed (DE) RNAs, including mRNAs, microRNAs (miRNAs), and long non-coding RNAs (lncRNAs), in stage I, II, III, and IV cervical cancer patients from the TCGA database to fully reveal the dynamic changes caused by cervical cancer.

Results: First, DE RNAs in cervical cancer tissues from stage I, II, III, and IV patients and normal cervical tissues were identified and divided into different profiles. Read More

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Identification of NY-ESO-1 Specific Murine T Cell Receptors With Distinct Recognition Pattern for Tumor Immunotherapy.

Front Immunol 2021 23;12:644520. Epub 2021 Mar 23.

Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China.

New York esophageal squamous cell carcinoma 1 (NY-ESO-1) is a promising target for T-cell receptor-engineered T cell (TCR-T) therapy, and targeting the human leukocyte antigen (HLA)-A2 restricted NY-ESO-1 epitope has yielded remarkable clinical benefits in the treatment of multiple advanced malignancies. Herein, we report the identification of two NY-ESO-1 epitope-specific murine TCRs obtained from HLA-A0201 transgenic mice. NY-ESO-1 specific TCRs were isolated after vaccinating HLA-A2 transgenic mice with epitope peptides. Read More

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Integrative Clinical, Molecular, and Computational Analysis Identify Novel Biomarkers and Differential Profiles of Anti-TNF Response in Rheumatoid Arthritis.

Front Immunol 2021 23;12:631662. Epub 2021 Mar 23.

Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Reina Sofia, Universidad de Cordoba, Córdoba, Spain.

This prospective multicenter study developed an integrative clinical and molecular longitudinal study in Rheumatoid Arthritis (RA) patients to explore changes in serologic parameters following anti-TNF therapy (TNF inhibitors, TNFi) and built on machine-learning algorithms aimed at the prediction of TNFi response, based on clinical and molecular profiles of RA patients. A total of 104 RA patients from two independent cohorts undergoing TNFi and 29 healthy donors (HD) were enrolled for the discovery and validation of prediction biomarkers. Serum samples were obtained at baseline and 6 months after treatment, and therapeutic efficacy was evaluated. Read More

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A Novel Grape-Derived Prebiotic Selectively Enhances Abundance and Metabolic Activity of Butyrate-Producing Bacteria in Faecal Samples.

Front Microbiol 2021 23;12:639948. Epub 2021 Mar 23.

Department of Biology, University of Girona, Girona, Spain.

Inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) patients have different faecal microbiota profiles compared to healthy controls. Prebiotics intake influences intestinal microbiota composition which in turn influence the growth of short-chain fatty acids (SCFA) producing bacteria. This study aimed to evaluate the capacity of Previpect, a new prebiotic obtained from grapes fibre, to balance the dysbiosis found in patients with intestinal disorders. Read More

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Profiles of 5α-Reduced Androgens in Humans and Eels: 5α-Dihydrotestosterone and 11-Ketodihydrotestosterone Are Active Androgens Produced in Eel Gonads.

Front Endocrinol (Lausanne) 2021 23;12:657360. Epub 2021 Mar 23.

Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.

Although 11-ketotestosterone (11KT) and testosterone (T) are major androgens in both teleosts and humans, their 5α-reduced derivatives produced by steroid 5α-reductase (SRD5A/srd5a), i.e., 11-ketodihydrotestosterone (11KDHT) and 5α-dihydrotestosterone (DHT), remains poorly characterized, especially in teleosts. Read More

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Symbolic Analysis of the Heart Rate Variability During the Plateau Phase Following Maximal Sprint Exercise.

Front Physiol 2021 23;12:632883. Epub 2021 Mar 23.

Department of Pathophysiology and Transplantation, Human Physiology Section, University of Milan, Milan, Italy.

Cardiac autonomic control is commonly assessed the analysis of fluctuations of the temporal distance between two consecutive R-waves (RR). Cardiac regulation assessment following high intensity physical exercise is difficult due to RR non-stationarities. The very short epoch following maximal sprint exercise when RR remains close to its lowest value, i. Read More

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Circular RNA CircEPB41L2 Functions as Tumor Suppressor in Hepatocellular Carcinoma Through Sponging miR-590-5p.

Cancer Manag Res 2021 1;13:2969-2981. Epub 2021 Apr 1.

The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, People's Republic of China.

Background: Circular RNAs (circRNAs) could interact with miRNAs to regulate gene expression, participating in hepatocellular carcinoma (HCC) initiation and development. This work aimed to determine the potential function and molecular mechanism of circEPB41L2 (hsa_circ_0077837) during HCC progression.

Materials And Methods: The expression of circEPB41L2 in HCC tissues and HCC cell lines was quantified using real-time quantitative PCR (qRT-PCR). Read More

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is a Novel Prognostic Marker for Diffuse Large B-Cell Lymphoma.

Pharmgenomics Pers Med 2021 1;14:397-408. Epub 2021 Apr 1.

Department of Hematology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

Purpose: Diffuse large B-cell lymphoma (DLBCL) is the most common B-cell malignancy. Thirty to forty percent of DLBCL patients still experience relapse or develop refractory disease even with standard immunochemotherapy, leading to a poor prognosis. Currently, although several gene-based classification methods can be used to predict the prognosis of DLBCL, some patients are still unable to be classified. Read More

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Identification of Metabolic-Associated Genes for the Prediction of Colon and Rectal Adenocarcinoma.

Onco Targets Ther 2021 31;14:2259-2277. Epub 2021 Mar 31.

Public Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin, 300060, People's Republic of China.

Background And Aim: Uncontrolled proliferation is the most prominent biological feature of tumors. In order to rapidly proliferate, tumor cells regulate their metabolic behavior by controlling the expression of metabolism-related genes (MRGs) to maximize the utilization of available nutrients. In this study, we aimed to construct prognosis models for colorectal adenocarcinoma (COAD) and rectum adenocarcinoma (READ) using MRGs to predict the prognoses of patients. Read More

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Gene expression profiles of YAP1, TAZ, CRB3, and VDR in familial and sporadic multiple sclerosis among an Iranian population.

Sci Rep 2021 Apr 8;11(1):7713. Epub 2021 Apr 8.

Isfahan Neuroscience Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Alterations in the regulatory mechanisms that control the process of myelination in the nervous system, may lead to the impaired myelination in the Multiple sclerosis. The Hippo pathway is an important mediator of myelination in the nervous system and might contribute to the pathophysiology of MS. This study examined via qPCR the RNA expression of YAP1, TAZ, and CRB3 as the key effectors of the Hippo pathway and also, VDR in the peripheral blood of 35 sporadic, 37 familial MS patients; and also 34 healthy first-degree relatives of the familial MS patients (HFR) and 40 healthy individuals without a family history of the disease (control). Read More

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Frailty: the current challenge for aging people with HIV.

Curr Opin HIV AIDS 2021 Apr 7. Epub 2021 Apr 7.

Division of Geriatric Medicine, Comprehensive HIV Aging Initiative, Combined Viral Illness Service, Division of Infectious Diseases, McGill University Health Center, Quebec, Canada Division of Geriatrics, Hospital Universitario Infanta Leonor, Madrid, Spain Divisions of Infectious Diseases and Geriatric Medicine, University of Colorado Denver-Anschutz Medical Campus, Aurora, Colorado, USA.

Purpose Of Review: Older adults account for the majority of people with HIV (PWH) in high-income countries and have increasingly complex clinical profiles related to premature aging. Frailty is an important geriatric syndrome affecting a minority of PHW. Frailty negatively affects PHW's clinical status and quality of life. Read More

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Comparative transcriptome analysis of transcripts of uncertain coding potential in septic myocardial depression.

BMC Cardiovasc Disord 2021 Apr 8;21(1):166. Epub 2021 Apr 8.

Department of Pediatrics, PICU, Shengjing Hospital of China Medical University, No. 36, SanHao Street, Shenyang City, 110004, Liaoning Province, People's Republic of China.

Background: Septic shock with myocardial depression is very common in intensive care units. However, the exact molecular mechanisms underlying sepsis-induced myocardial depression remain unclear. Whether the profiles of transcripts of uncertain coding potential (TUCPs) differ between patients with and without myocardial depression is also unknown. Read More

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