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Comprehensive analysis of Syk gene methylation in colorectal cancer.

Immun Inflamm Dis 2021 May 12. Epub 2021 May 12.

Department of Gastrointestinal Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.

Background: Metastasis of colorectal cancer (CRC) extremely affects the prognosis of CRC patients. Recently, the genetic methylation has been shown to associate with tumor metastasis. This research aimed to explore the Syk gene, which is frequently hypermethylated in different cancers, and its impact on the metastasis of CRC cells. Read More

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Spatiotemporal regulation of galectin-1-induced T-cell death in lamina propria from Crohn's disease and ulcerative colitis patients.

Apoptosis 2021 May 12. Epub 2021 May 12.

Instituto de Estudios Inmunológicos y Fisiopatológicos-IIFP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, Argentina.

Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), is characterized by chronic, relapsing intestinal inflammation. Galectin-1 (Gal-1) is an endogenous lectin with key pro-resolving roles, including induction of T-cell apoptosis and secretion of immunosuppressive cytokines. Despite considerable progress, the relevance of Gal-1-induced T-cell death in inflamed tissue from human IBD patients has not been ascertained. Read More

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Dynamics of the enterohepatic circulation of bile acids in healthy humans.

Am J Physiol Gastrointest Liver Physiol 2021 May 12. Epub 2021 May 12.

Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Germanygrid.15090.3d.

Regulation of bile acid metabolism is normally discussed as the regulation of bile acid synthesis, which serves to compensate for intestinal loss in order to maintain a constant pool size. After a meal, bile acids start cycling in the enterohepatic circulation. Farnesoid X receptor-dependent ileal and hepatic processes lead to negative feedback inhibition of bile acid synthesis. Read More

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Resveratrol improved hippocampal neurogenesis following lead exposure in rats through activation of SIRT1 signaling.

Environ Toxicol 2021 May 12. Epub 2021 May 12.

Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, China.

Lead (Pb) poses a potential environmental risk factor for cognitive dysfunction during early life and childhood. Resveratrol is considered a promising antioxidant with respect to the prevention of cognitive deficits and act as a potent SIRT1 agonist. Here in, this study aims to investigate the profile of neurogenesis markers following Pb exposure and to determine the regulatory role of resveratrol in this process. Read More

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Comprehensive analysis for detecting radiation-specific molecules expressed during radiation-induced rat thyroid carcinogenesis.

J Radiat Res 2021 May;62(Supplement_1):i78-i87

Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Although the association between radiation exposure and thyroid carcinogenesis is epidemiologically evident, 'true' radiation-induced cancers cannot be identified from biological evidence of radiation-associated cases. To assess the individual risk for thyroid cancer due to radiation exposure, we aimed to identify biomarkers that are specifically altered during thyroid carcinogenesis after irradiation in a time-dependent manner in an animal model. Thyroid glands were obtained from rats (n = 175) at 6-16 months after local X-ray (0. Read More

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DNA methylation is involved in acclimation to iron deficiency in rice (Oryza sativa).

Plant J 2021 May 12. Epub 2021 May 12.

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China.

Iron (Fe) is an essential micronutrient in plants and Fe limitation significantly affects plant growth, yield and food quality. While many studies have reported the transcriptomic profile and pursue molecular mechanism in response to Fe limitation, little is known if epigenetic factors play a role in response to Fe deficiency. In this study, whole-genome bisulfite sequencing analysis, high throughput RNA-seq of mRNA, small RNA and transposable element (TE) expression with root and shoot organs of rice seedlings under Fe-sufficient and Fe-deficient conditions were performed. Read More

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Novel and known miRNAs in zebu (Tharparkar) and crossbred (Karan-Fries) cattle under heat stress.

Funct Integr Genomics 2021 May 11. Epub 2021 May 11.

Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.

MicroRNAs (miRNAs) are small single-stranded non-coding RNAs that act as the master regulator of animal growth and development. RNA-RNA interaction is an important mechanism of gene regulation during biotic and abiotic stress. Heat stress (HS) is one of the most important abiotic factors which affect the growth, milk yield and reproductive health of the dairy animals. Read More

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Correction to: Cyclically stretched ACL fibroblasts emigrating from spheroids adapt their cytoskeleton and ligament-related expression profile.

Cell Tissue Res 2021 May 11. Epub 2021 May 11.

Institute of Anatomy and Cell Biology, Paracelsus Medical University, Prof.-Ernst-Nathan Str. 1, 90419 Nuremberg and Salzburg, Nuremberg, Germany.

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Leukocytes from Patients with Drug-Sensitive and Multidrug-Resistant Tuberculosis Exhibit Distinctive Profiles of Chemokine Receptor Expression and Migration Capacity.

J Immunol Res 2021 21;2021:6654220. Epub 2021 Apr 21.

Laboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias "Ismael Cosío Villegas", Mexico City, Mexico.

Tuberculosis (TB), caused by (Mtb), remains as a leading infectious cause of death worldwide. The increasing number of multidrug-resistant TB (MDR-TB) cases contributes to the poor control of the TB epidemic. Currently, little is known about the immunological requirements of protective responses against MDR-TB. Read More

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RNA-binding protein YBX1 promotes cell proliferation and invasiveness of nasopharyngeal carcinoma cells binding to AURKA mRNA.

J Cancer 2021 7;12(11):3315-3324. Epub 2021 Apr 7.

The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.

RNA-binding proteins (RBPs) play essential roles in post-transcriptional control of gene expression. Dysregulation of RBPs is intensively implicated in development and progression of human diseases, including cancers. However, the roles of RBPs in nasopharyngeal carcinoma (NPC), which is a distinct subtype of head and neck cancer, remain elusive. Read More

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Identification of an apoptosis-related prognostic gene signature and molecular subtypes of clear cell renal cell carcinoma (ccRCC).

J Cancer 2021 2;12(11):3265-3276. Epub 2021 Apr 2.

The Fifth Hospital of Xiamen, Xiamen 361101, Fujian Province, China.

Previously studies have shown that apoptosis-related genes play an essential role in normal cell turnover, maintaining the immune system function, and inducing cell death. However, their prognostic roles in clear cell renal cell carcinoma (ccRCC) have not been thoroughly investigated. In the present study, apoptosis-related genes expression profiles from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) database were used as training dataset and external validation dataset, respectively. Read More

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A Stat1 bound enhancer promotes Nampt expression and function within tumor associated macrophages.

Nat Commun 2021 May 11;12(1):2620. Epub 2021 May 11.

Division of Microbiology and Immunology, Department of Pathology, University of Utah, Salt Lake City, UT, USA.

Tumor associated macrophage responses are regulated by distinct metabolic states that affect their function. However, the ability of specific signals in the local tumor microenvironment to program macrophage metabolism remains under investigation. Here, we identify NAMPT, the rate limiting enzyme in NAD salvage synthesis, as a target of STAT1 during cellular activation by interferon gamma, an important driver of macrophage polarization and antitumor responses. Read More

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Transcriptomics analysis of differentially expressed genes in subcutaneous and perirenal adipose tissue of sheep as affected by their pre- and early postnatal malnutrition histories.

BMC Genomics 2021 May 11;22(1):338. Epub 2021 May 11.

Nutrition Research Unit, Department of Animal Science, Aarhus University, Blichers Alle 20, 8830, Tjele, Denmark.

Background: Early life malnutrition is known to target adipose tissue with varying impact depending on timing of the insult. This study aimed to identify differentially expressed genes in subcutaneous (SUB) and perirenal (PER) adipose tissue of 2.5-years old sheep to elucidate the biology underlying differential impacts of late gestation versus early postnatal malnutrition on functional development of adipose tissues. Read More

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Circulating microRNA profile unveils mechanisms of action of acitretin for psoriasis vulgaris.

Bioengineered 2021 Dec;12(1):1838-1850

Department of Dermatology, The Second Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan Province, China.

Psoriasis vulgaris is a common chronic and recurrent inflammatory skin disease. In clinical practice, acitretin is the first-line treatment drug for psoriasis vulgaris. MicroRNAs (miRNAs) play a vital role in the initiation and development of psoriasis vulgaris. Read More

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December 2021

CD200 Limits Monopoiesis and Monocyte Recruitment in Atherosclerosis.

Circ Res 2021 May 12. Epub 2021 May 12.

Kennedy Institue of Rheumatology, University of Oxford, UNITED KINGDOM.

Inflammation is a basic component of the pathogenesis of atherosclerosis. CD200 is an immune checkpoint known to control macrophage activation. CD200 recently emerged in the Framingham Heart Study and 2 other cohorts as being potentially relevant in CVD. Read More

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Differential expression of insulin receptor substrate-1(IRS-1) in visceral and subcutaneous adipose depots of morbidly obese subjects undergoing bariatric surgery in a tertiary care center in north India; SNP analysis and correlation with metabolic profile.

Diabetes Metab Syndr 2021 May 5;15(3):981-986. Epub 2021 May 5.

Department of Biochemistry, All India Institute of Medical Sciences, Delhi, India. Electronic address:

Background: /aim: Abdominal obesity and associated metabolic consequences are a burgeoning problem in Asian Indians and studying their genetic predisposition is important. This study is aimed at assessing variations in Insulin receptor substrate-1 (IRS-1), its expression at regional fat-depots (visceral and subcutaneous) in morbidly obese patients, and correlation with genotype-phenotype traits.

Methods: Gene expression of IRS-1 in paired adipose tissue from 35 morbidly obese subjects (BMI) > 40 kg/m) with co-morbidities and 15 controls (BMI<25 kg/m), undergoing bariatric/elective abdominal surgery, respectively was determined by quantitative real time PCR. Read More

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Immune and barrier characterization of atopic dermatitis skin phenotype in Tanzanian patients.

Ann Allergy Asthma Immunol 2021 May 8. Epub 2021 May 8.

Laboratory of Inflammatory Skin Diseases, Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USA. Electronic address:

Background: Atopic dermatitis/AD is a common disease, with particularly high prevalence seen in Africa. It is increasingly recognized that AD patients of different ethnic backgrounds have unique molecular signatures in skin, potentially accounting for treatment response variations. However, the skin profile of AD patients from Africa is unknown, hindering development of new treatments targeted to this patient population. Read More

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mTOR pathway and somatostatin receptors expression intratumor-heterogeneity in ileal NETs.

Endocr Relat Cancer 2021 May 1. Epub 2021 May 1.

M Fassan, Department of Medicine, University of Padua, Padova, Italy.

The knowledge of the molecular landscape of ileal neuroendocrine tumors (NETs) is affected by the lack of systematic studies investigating intra-tumoral heterogeneity. In this study, intra-tumoral heterogeneity was investigated in 27 primary ileal G1-NETs and their matched nodal and liver metastases in order to assess the tumor grading, the expression status of 2 somatostatin receptor isoforms (i.e. Read More

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Genetic and Histopathological Alterations in Caco-2 and HuH-7 Cells Treated with Secondary Metabolites of Marine fungi.

J Gastrointest Cancer 2021 May 11. Epub 2021 May 11.

Department of Botany and Microbiology, Faculty of Science, Al-Azhar Uniersity, 11847, Nasr City, Cairo, Egypt.

The present work aimed to study the activity of naturally derived fungal secondary metabolites as anticancer agents concerning their cytotoxicity, apoptotic, genetic, and histopathological profile. It was noticed that Aspergillus terreus, Aspergillus flavus, and Aspergillus fumigatus induced variable toxic potential that was cell type, secondary metabolite type, and concentration dependent. Human colonic adenocarcinoma cells (Caco-2) showed less sensitivity than hepatocyte-derived cellular carcinoma cells (HuH-7), and in turn, the half-maximal inhibitory concentration (IC) was variable. Read More

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Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae).

J Insect Sci 2021 May;21(3)

Southern Insect Management Research Unit, USDA, Agricultural Research Service, Stoneville, MS 38776.

Host plant preference of agricultural pests may shift throughout the growing season, allowing the pests to persist on wild hosts when crops are not available. Lygus Hahn (Hemiptera: Miridae) bugs are severe pests of cotton during flowering and fruiting stages, but can persist on alternative crops, or on weed species. Diversity of digestive enzymes produced by salivary glands and gut tissues play a pivotal role in an organism's ability to utilize various food sources. Read More

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How COVID-19 Has Globalized: Unknown Origin, Rapid Transmission, and the Immune System Nourishment.

Adv Exp Med Biol 2021 ;1318:119-147

Research Center for Immunodeficiencies, Children's Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Iran.

The novel coronavirus disease (COVID-19) profoundly influences T-cell immunity. The counts of total T cells and T-cell subsets, especially CD4+ and CD8+ T cells, are decreased in patients with COVID-19. Also, the function of these cells becomes less effective as the expression of immune inhibitory receptors, such as Tim3 and PD-1, increases over time during the disease. Read More

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CCR2-dependent monocyte-derived cells restrict SARS-CoV-2 infection.

bioRxiv 2021 May 4. Epub 2021 May 4.

SARS-CoV-2 has caused a historic pandemic of respiratory disease (COVID-19) and current evidence suggests severe disease is associated with dysregulated immunity within the respiratory tract. However, the innate immune mechanisms that mediate protection during COVID-19 are not well defined. Here we characterize a mouse model of SARS-CoV-2 infection and find that early CCR2-dependent infiltration of monocytes restricts viral burden in the lung. Read More

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Identification and characterization of a SARS-CoV-2 specific CD8 T cell response with immunodominant features.

Nat Commun 2021 05 10;12(1):2593. Epub 2021 May 10.

Division of Molecular Oncology and Immunology, The Netherlands Cancer Institute, Amsterdam, North Holland, The Netherlands.

The COVID-19 pandemic caused by SARS-CoV-2 is a continuous challenge worldwide, and there is an urgent need to map the landscape of immunogenic and immunodominant epitopes recognized by CD8 T cells. Here, we analyze samples from 31 patients with COVID-19 for CD8 T cell recognition of 500 peptide-HLA class I complexes, restricted by 10 common HLA alleles. We identify 18 CD8 T cell recognized SARS-CoV-2 epitopes, including an epitope with immunodominant features derived from ORF1ab and restricted by HLA-A*01:01. Read More

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Mutational Activation of Antibiotic Resistant Mechanisms in the Absence of Major Drug Efflux Systems of .

J Bacteriol 2021 May 10. Epub 2021 May 10.

School of Life Sciences, Arizona State University, Tempe, AZ 85287

Mutations are one of the common means by which bacteria acquire resistance to antibiotics. In an mutant lacking major antibiotic efflux pumps AcrAB and AcrEF, mutations can activate alternative pathways that lead to increased antibiotic resistance. In this work, we isolated and characterized compensatory mutations of this nature mapping in four different regulatory genes-, , , or The gain-of-function mutations in constitutively activated the BaeSR two-component regulatory system to increase the expression of the MdtABC efflux pump. Read More

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Genome wide analysis of kinesin gene family in Citrullus lanatus reveals an essential role in early fruit development.

BMC Plant Biol 2021 May 10;21(1):210. Epub 2021 May 10.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Background: Kinesin (KIN) as a motor protein is a versatile nano-machine and involved in diverse essential processes in plant growth and development. However, the kinesin gene family has not been identified in watermelon, a valued and nutritious fruit, and yet their functions have not been characterized. Especially, their involvement in early fruit development, which directly determines the size, shape, yield and quality of the watermelon fruit, remains unclear. Read More

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Integrative-omics for discovery of network-level disease biomarkers: a case study in Alzheimer's disease.

Brief Bioinform 2021 May 10. Epub 2021 May 10.

Department of BioHealth Informatics, Indiana University Purdue University Indianapolis, 420 University Blvd, Indianapolis, IN 46204, USA.

A large number of genetic variations have been identified to be associated with Alzheimer's disease (AD) and related quantitative traits. However, majority of existing studies focused on single types of omics data, lacking the power of generating a community including multi-omic markers and their functional connections. Because of this, the immense value of multi-omics data on AD has attracted much attention. Read More

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An autoantigen profile of human A549 lung cells reveals viral and host etiologic molecular attributes of autoimmunity in COVID-19.

J Autoimmun 2021 Apr 27;120:102644. Epub 2021 Apr 27.

Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, USA; Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, USA. Electronic address:

We aim to establish a comprehensive COVID-19 autoantigen atlas in order to understand autoimmune diseases caused by SARS-CoV-2 infection. Based on the unique affinity between dermatan sulfate and autoantigens, we identified 348 proteins from human lung A549 cells, of which 198 are known targets of autoantibodies. Comparison with current COVID data identified 291 proteins that are altered at protein or transcript level in SARS-CoV-2 infection, with 191 being known autoantigens. Read More

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The transcriptomic profile of endometrial receptivity in recurrent miscarriage.

Eur J Obstet Gynecol Reprod Biol 2021 Apr 30;261:211-216. Epub 2021 Apr 30.

Tommy's National Centre for Miscarriage Research, Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK.

Objective: To characterise the endometrial transcriptomic profiles of women who suffered recurrent miscarriage and to set the foundation for the development of an endometrial receptivity test that could predict the fate of subsequent pregnancies.

Study Design: This was a prospective multicentre cohort study performed at the Tommy's National Centre for Miscarriage Research in Birmingham, Saint Mary's Hospital in Manchester and Royal Devon & Exeter Hospital, United Kingdom. The study was conducted between December 2017 and December 2019. Read More

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The association between the expression of nuclear Yes-associated protein 1 (YAP1) and p53 protein expression profile in breast cancer patients.

PLoS One 2021 10;16(5):e0250986. Epub 2021 May 10.

Division of Breast Surgery, Department of Surgery, Seoul St Mary's Hospital, College of Medicine, The Catholic University of Seoul, Seoul, Korea.

Background: Yes-associated protein 1 (YAP1) is a key effector molecule regulated by the Hippo pathway and described as a poor prognostic factor in breast cancer. Tumor protein 53 (TP53) mutation is well known as a biomarker related to poor survival outcomes. So far clinical characteristics and survival outcome according to YAP1 and TP53 mutation have been poorly identified in breast cancer. Read More

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Role of IL-36γ/IL-36R Signaling in Corneal Innate Defense Against Candida albicans Keratitis.

Invest Ophthalmol Vis Sci 2021 May;62(6):10

Departments of Ophthalmology and Anatomy and Cell Biology Wayne State University School of Medicine, Detroit, Michigan, United States.

Purpose: Interleukin (IL)-36 cytokines have been shown to play either beneficial or detrimental roles in the infection of mucosal tissues in a pathogen-dependent manner, but their involvement in fungal keratitis remains elusive. We herein investigated their expression and function in mediating corneal innate immunity against Candida albicans infection.

Methods: Gene expression in mouse corneas with or without C. Read More

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