Gene 2021 Apr 9:145640. Epub 2021 Apr 9.
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, 266000, Qingdao, China; University of Chinese Academy of Sciences, 100039, Beijing, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, 7 Nanhai Road, 266071, Qingdao, China. Electronic address:
Obtaining detectable knockout phenotypes in the G0 generation is essential for gene function studies. Although CRISPR/Cas9-mediated gene editing has been employed to knock out molluscan genes, detectable phenotypes in the G0 generation have not been reported in these animals. In this study, we determined the knockout phenotype of a cilium-related gene, calaxin, using CRISPR/Cas9 technology in the gastropod mollusk Lottia goshimai. Read More