30 results match your criteria produced 14500

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Genomic prediction for growth using a low-density SNP panel in dromedary camels.

Sci Rep 2021 Apr 7;11(1):7675. Epub 2021 Apr 7.

Persian BayanGene Research and Training Center, 7134767617, Shiraz, Iran.

For thousands of years, camels have produced meat, milk, and fiber in harsh desert conditions. For a sustainable development to provide protein resources from desert areas, it is necessary to pay attention to genetic improvement in camel breeding. By using genotyping-by-sequencing (GBS) method we produced over 14,500 genome wide markers to conduct a genome- wide association study (GWAS) for investigating the birth weight, daily gain, and body weight of 96 dromedaries in the Iranian central desert. Read More

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Toxicological evaluation of an unextracted Aurantiochytrium limacinum biomass, a novel docosahexaenoic acid rich feed ingredient.

Food Chem Toxicol 2020 Jul 13;141:111397. Epub 2020 May 13.

Alltech France SARL, Rue Charles Amand, 14500, Vire, France.

The purpose of this paper was to evaluate the toxicological potential of a heterotrophically grown unextracted Aurantiochytrium limacinum biomass (AURA) when used as a food additive. The following toxicological assessments were conducted on this novel docosahexaenoic acid rich feed ingredient: Mutagenic activity was tested by means of the Ames' test using five Salmonella typhimurium strains; clastogenic activity was investigated using the micronucleus test in male and female Sprague Dawley rats; genotoxic activity was assessed by means of the in vitro metaphase analysis tests in human lymphocytes; oral toxicity was tested by administration of AURA at various concentrations; eye and skin irritation was assessed in rabbits according to OECD guidelines; skin sensitivity was established in guinea pigs by means of the Buehler test. All conducted tests were considered valid under the experimental conditions. Read More

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Design and validation of a real-time PCR technique for assessing the level of inclusion of fungus- and yeast-based additives in feeds.

J Microbiol Methods 2020 04 13;171:105867. Epub 2020 Feb 13.

Alltech SARL, Rue Charles Amand, 14500 Vire, France.

A reliable method for quantification of non-viable microbe-based nutritional and zootechnical additives introduced into feed is essential in order to ensure regulatory compliance, feed safety and product authenticity in industrial applications. In the present work, we developed a novel real-time quantitative polymerase chain reaction (qPCR) -based analysis protocol for monitoring two microbial additives in feed matrices. To evaluate the applicability of the method, pelleted wheat- and maize-based broiler chicken diets containing a non-viable phytase-producing strain of Aspergillus niger produced in solid state fermentation (150 or 300 g/t) and a non-viable selenium-enriched Saccharomyces cerevisiae (100 or 200 g/t) as model feed ingredients, were manufactured and subjected to analysis. Read More

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Proof of concept of high-rate decentralized pre-composting of kitchen waste: Optimizing design and operationof a novel drum reactor.

Waste Manag 2019 May 29;91:20-32. Epub 2019 Apr 29.

Research Group of Sustainable Air, Energy and Water Technology, Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium. Electronic address:

Each ton of organic household waste that is collected, transported and composted incurs costs (€75/ton gate fee). Reducing the mass and volume of kitchen waste (KW) at the point of collection can diminish transport requirements and associated costs, while also leading to an overall reduction in gate fees for final processing. To this end, the objective of this research was to deliver a proof of concept for the so-called "urban pre-composter"; a bioreactor for the decentralized, high-rate pre-treatment of KW, that aims at mass and volume reduction at the point of collection. Read More

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Platinum and Rhodium in Potato Samples by Using Voltammetric Techniques.

Foods 2019 Feb 5;8(2). Epub 2019 Feb 5.

Chimiche e Farmaceutiche, Dipartimento di Scienze e Tecnologie Biologiche, Università di Palermo, Viale delle Scienze, 90128 Palermo, Italy.

Potato is a starchy, tuberous crop from the perennial having high nutritional values. This paper is the first analytical approach to quantify Pt and Rh in vegetal food. In this study a total of 38 different potato samples produced in Europe and one in Australia were investigated. Read More

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February 2019

Convenient and Universal Fabrication Method for Antibody-Enzyme Complexes as Sensing Elements Using the SpyCatcher/SpyTag System.

Anal Chem 2018 12 27;90(24):14500-14506. Epub 2018 Nov 27.

Joint Department of Biomedical Engineering , University of North Carolina at Chapel Hill and North Carolina State University , Chapel Hill , North Carolina 27599 , United States.

Antibody-enzyme complexes (AECs) are ideal sensing elements, especially when oxidoreductases are used as the enzymes in the complex, with the potential to carry out rapid electrochemical measurements. However, conventional methods for the fabrication of AECs, including direct fusion and chemical conjugation, are associated with issues regarding the generation of insoluble aggregates and production of homogeneous AECs. Here, we developed a convenient and universal method for the fabrication of homogeneous AECs using the SpyCatcher/SpyTag system. Read More

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December 2018

T cells upon activation promote endothelin 1 production in monocytes via IFN-γ and TNF-α.

Sci Rep 2017 11 3;7(1):14500. Epub 2017 Nov 3.

Division of Rheumatology and Allergology, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan.

Endothelin 1 (ET-1), mainly produced from vascular endothelial cells, induces vasoconstriction in physiological conditions. The endothelin receptor antagonist is among the most effective agents for pulmonary hypertension. However, little is known about the production source of ET-1 in inflammation and immunity. Read More

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November 2017

Stone tool production and utilization by bonobo-chimpanzees (Pan paniscus).

Proc Natl Acad Sci U S A 2012 Sep 21;109(36):14500-3. Epub 2012 Aug 21.

International Graduate Center of Evolution, Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel.

Using direct percussion, language-competent bonobo-chimpanzees Kanzi and Pan-Banisha produced a significantly wider variety of flint tool types than hitherto reported, and used them task-specifically to break wooden logs or to dig underground for food retrieval. For log breaking, small flakes were rotated drill-like or used as scrapers, whereas thick cortical flakes were used as axes or wedges, leaving consistent wear patterns along the glued slits, the weakest areas of the log. For digging underground, a variety of modified stone tools, as well as unmodified flint nodules, were used as shovels. Read More

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September 2012

Synthesis and structure of aluminium amine-phenolate complexes.

Dalton Trans 2009 Jul 8(28):5551-8. Epub 2009 Jun 8.

Department of Chemistry, University of Bath, Bath, UK BA2 7AY.

Reaction of Al(OiPr)3 with the tris-phenol amine ligand L1H3 in toluene at ambient temperature results in the formation of the iso-propanol adduct [HOiPr.Al(L1)]. Single crystal X-ray diffraction analysis reveals the structure to be a hydrogen bonded dimer. Read More

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In vitro effects on proliferation, apoptosis and colony inhibition in ER-dependent and ER-independent human breast cancer cells by selected mushroom species.

Oncol Rep 2006 Feb;15(2):417-23

Bastyr University, 14500 Juanita Drive NE, Kenmore, WA 98028, USA.

Breast cancer is the most commonly diagnosed cancer among women in Western countries. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. We have screened 38 species of edible mushroom on human estrogen-receptor positive (MCF-7) and estrogen-receptor negative (MDA-MB-231, BT-20) breast cancer cells to select potential agents with broad-spectrum antitumor activity against breast cancer cells. Read More

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February 2006

Computerized video time-lapse (CVTL) analysis of the fate of giant cells produced by X-irradiating EJ30 human bladder carcinoma cells.

Radiat Res 2003 Jun;159(6):705-12

Radiation Oncology Research Laboratory, University of California San Francisco, 94103, USA.

This study was designed to examine the viability and proliferation of uninucleated and multinucleated giant cells formed after 6 Gy X irradiation. The pedigrees of 102 individual EJ30 giant cells present 5 days after irradiation were analyzed from time-lapse movies captured over 6.3 days from 100 fields (100x). Read More

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Metabolism of homoharringtonine, a cytotoxic component of the evergreen plant Cephalotaxus harringtonia.

J Exp Ther Oncol 2003 Jan-Feb;3(1):47-52

Department of Experimental Therapeutics, Unit 601, University of Texas M.D. Anderson Cancer Center, 8000 El Rio, Houston, TX 77054, USA.

Homoharringtonine (HHT), first isolated from the Chinese evergreen Cephalotaxus harringtonia, has been demonstrated to have a broad antitumor activity in rodents and antileukemic effects in humans. We found that HHT was metabolized to an acid product [HHT-acid; 2'-hydroxy-2'-(alpha-acetic acid)-6'-hydroxy-6'-methylheptanoyl cephalotaxine] when incubated with either human plasma or mouse plasma in vitro. The conversion was faster, however, in mouse plasma, and was both time- and temperature-dependent. Read More

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February 2004

Aerobic waste activated sludge (WAS) for start-up seed of mesophilic and thermophilic anaerobic digestion.

Water Res 2002 Sep;36(15):3860-6

Department of Civil & Environmental Engineering, Vanderbilt University, P.O. Box 1831, Station B, Nashville, TN 37235, USA.

Since there are very limited numbers of thermophilic anaerobic digesters being operated, it is often difficult to start up a new one using sludge from an existing reactor as a seed. However, for obvious reasons it seems few attempts have been made to compare the start-up performance of thermophilic anaerobic digestion using different sources of seed sludges. The purpose of this study was to evaluate the start-up performance of anaerobic digestion using aerobic waste activated sludge (WAS) from a plant which has no anaerobic digesters and mesophilic anaerobic digested sludge (ADS) as the seed source at both mesophilic (35 degrees C) and thermophilic (55 degrees C) temperatures. Read More

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September 2002

Antagonism--no synergism--in pairwise tests of carcinogens in rats.

Michael Gough

Regul Toxicol Pharmacol 2002 Jun;35(3):383-92

Cato Institute, Washington, DC, USA.

In the mid-1970s, the National Cancer Institute issued a contract for the testing of a dozen chemicals, most of them known to be carcinogenic in animals, in pairwise combinations. Those tests, which involved 918 pairwise tests and over 14,500 laboratory rats, produced no good evidence for any synergistic interactions in which exposure to two chemicals resulted in a number of tumors greater than the number produced by exposure to either chemical alone. A number of tests resulted in antagonism, in which the number of tumors in animals exposed to a pair of carcinogens was less than the number seen after exposure to either carcinogen by itself. Read More

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Tetracycline-controlled expression of glycosylated porcine interferon-gamma in mammalian cells.

Anim Biotechnol 1999 ;10(1-2):63-79

Unité de Virologie et d'Immunologie Moléculaires, I.N.R.A., Jouy-en-Josas, France.

Tetracycline-controlled expression plasmids that allow inducible expression of proteins in mammalian cells (Gossen & Bujard, 1992), have been used to express porcine interferon-gamma in the RK-13 rabbit kidney cell line. Following neomycin selection, stable clones produced recombinant, glycosylated porcine interferon-gamma (rGPoIFN-gamma) only after removal of tetracycline (Tc). Southern blot analysis of one clone showed that approximately 50 copies of IFN-gamma cDNA were present in the cell genome. Read More

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February 2000

Chemokines regulate hippocampal neuronal signaling and gp120 neurotoxicity.

Proc Natl Acad Sci U S A 1998 Nov;95(24):14500-5

Department of Pharmacological and Physiological Sciences, University of Chicago, 947 E. 58th Street (MC 0926), Chicago, IL 60637, USA.

The HIV-1 envelope protein gp120 induces apoptosis in hippocampal neurons. Because chemokine receptors act as cellular receptors for HIV-1, we examined rat hippocampal neurons for the presence of functional chemokine receptors. Fura-2-based Ca imaging showed that numerous chemokines, including SDF-1alpha, RANTES, and fractalkine, affect neuronal Ca signaling, suggesting that hippocampal neurons possess a wide variety of chemokine receptors. Read More

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November 1998

Cross-reactivities of monoclonal antibodies to a myotoxin from the venom of the broad-banded copperhead (Agkistrodon contortrix laticinctus).

Toxicon 1993 Sep;31(9):1187-96

Department of Physiological Sciences, Oklahoma State University, Stillwater 74078.

A panel of murine monoclonal antibodies (MAbs) specific for a 14,500 mol. wt myotoxin of Agkistrodon contortrix laticinctus (broad-banded copperhead) venom was produced by cell fusion using the purified toxin as immunogen. The MAbs were used in immunoblotting and ELISA experiments to determine their immunological cross-reactivities. Read More

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September 1993

Detection of human pancreatic pro-phospholipase A2 activation using an immunoassay for the free activation peptide DSGISPR.

Ann Clin Biochem 1991 Sep;28 ( Pt 5):497-503

Department of Surgery, St George's Hospital Medical School, London, UK.

There are several forms of the enzyme phospholipase A2 (PLA2) in human tissues. In the pancreas the enzyme is produced as a zymogen, pro-phospholipase A2 (pro-PLA2). The active form is generated upon proteolytic cleavage of the N-terminal prophospholipase A2 activation peptide (PLAP), with the sequence Asp-Ser-Gly-Ile-Ser-Pro-Arg (DSGISPR). Read More

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September 1991

Effects of beta-xylosides on proteoglycan biosynthesis and morphology of PC12 pheochromocytoma cells and primary cultures of rat cerebellum.

J Cell Sci 1991 Jun;99 ( Pt 2):237-46

Department of Pharmacology, State University of New York, Brooklyn 11203.

We have examined the effects of beta-xylosides, which act as exogenous acceptors for glycosaminoglycan chain initiation, on the morphology and proteoglycan biosynthesis of PC12 pheochromocytoma cells, and on monolayer, aggregate and explant cultures of early postnatal rat cerebellum. PC12 cells cultured for 13 days in the presence of nerve growth factor (NGF) and beta-xyloside, and labeled during days 11-13 with sodium [35S]sulfate, showed an 8- to 11-fold increase in [35S]sulfate-labeled macromolecules released into the culture medium. Most of the increase was accounted for by chondroitin sulfate, which was in the form of free glycosaminoglycan chains, which were not acid-precipitable. Read More

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Lethal potency and fractionation of Duvernoy's secretion from the brown tree snake, Boiga irregularis.

Toxicon 1991 ;29(4-5):401-7

Dept of Toxinology, U.S. Army Medical Research, Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-25011.

The liquid secretion contained only 15% protein and had relatively low proteolytic activity. The reconstituted crude secretion had a murine i.p. Read More

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September 1991

An oligosaccharide-tetanus toxoid conjugate vaccine against type III group B Streptococcus.

J Biol Chem 1990 Oct;265(30):18278-83

Channing Laboratory, Brigham and Women's Hospital, Boston, Massachusetts 02115.

We have developed an oligosaccharide-tetanus toxoid conjugate vaccine against type III group B Streptococcus. Purified group B streptococcal type III capsular polysaccharide was depolymerized by enzymatic digestion using endo-beta-galactosidase produced by Citrobacter freundii. Following enzymatic digestion, oligosaccharides were fractionated by gel filtration chromatography on Sephadex G-75. Read More

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October 1990

Granulocyte-macrophage colony stimulating factor from human lymphocytes. The effect of glycosylation on receptor binding and biological activity.

J Biol Chem 1990 Mar;265(8):4483-91

Melbourne Tumour Biology Branch, Ludwig Institute for Cancer Research, Victoria, Australia.

Native human granulocyte-macrophage colony stimulating factor (hGM-CSF) has previously been purified using methods which typically required several sequential chromatographic steps and only yielded small amounts of hGM-CSF. We have purified and characterized hGM-CSF using monoclonal antibodies raised against bacterially synthesized hGM-CSF. Activated donor T-lymphocytes grown in interleukin-2 and then reactivated with phytohemagglutinin produce several forms of hGM-CSF which can be purified using immunoaffinity absorption followed by reversed phase high performance liquid chromatography. Read More

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Hypoxia-induced increases in pulmonary transvascular protein escape in rats. Modulation by glucocorticoids.

J Clin Invest 1988 Dec;82(6):1840-7

Cardiovascular Pulmonary Research Laboratory, University of Colorado Health Sciences Center, Denver 80262.

Pulmonary edema after ascent to altitude is well recognized but its pathogenesis is poorly understood. To determine whether altitude exposure increases lung vascular permeability, we exposed rats to a simulated altitude of approximately 14,500 feet (barometric pressure [Pb] 450 Torr) and measured the pulmonary transvascular escape of radiolabeled 125I-albumin corrected for lung blood content with 51Cr-tagged red blood cells (protein leak index = PLI). Exposures of 24 and 48 h caused significant increases in PLI (2. Read More

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December 1988

Protein phosphorylation associated with stimulation of rabbit gastric glands.

Biochim Biophys Acta 1987 Sep;930(2):209-19

Changes in protein phosphorylation associated with stimulation of acid secretion were investigated using isolated rabbit gastric glands labeled with 32P. The glands were stimulated by 100 microM histamine plus either 10 microM forskolin or 50 microM isobutylmethylxanthine, homogenized, and fractionated into a series of pellets: 40 X g, 5 min; 4000 X g, 10 min; 14,500 X g, 10 min; 48,200 X g, 90 min (microsomes); and supernatant. Stimulation induced a redistribution of H+/K+-transporting ATPase among the membrane fractions, i. Read More

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September 1987

Natural murine interferon-gamma. Evidence for post-translational proteolytic processing.

J Biol Chem 1985 Aug;260(17):9936-40

Murine interferon-gamma (MuIFN-gamma), produced by the T-cell lymphoma, L12-R4, and stimulated with phorbol myristic acetate, was purified by rat monoclonal antibodies AN-18.17.24. Read More

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[Borna virus infection (Borna disease) in naturally and experimentally infected animals: its significance for research and practice].

Tierarztl Prax 1985 ;13(4):421-53

In this survey article on Borna Disease-many years after the review of Zwick (1939)-again a modern comprehensive summary of "Borna Disease virus infection" is given. The infection occurs in horses and sheep, furthermore, in laboratory animal species inoculated experimentally; its clinical, virological and neuropathological features have been described in numerous presentations. Clinical symptoms in naturally and experimentally infected animals are characterized by initial alterations in the sensorium. Read More

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Purification to homogeneity of biologically active human interleukin 1.

T Krakauer

Prep Biochem 1984-1985;14(5):449-70

Human interleukin 1 (IL-1) produced by lipopolysaccharide-stimulated monocytes was purified to homogeneity with retention of biological activity. IL-1 was measured by its ability to enhance the proliferative response of thymocytes to phytohemagglutinin. The purification procedure included hydrophobic affinity chromatography on phenyl Sepharose, gel filtration through Ultrogel AcA54 and preparative isoelectric focusing. Read More

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Production and evaluation of secretin antibodies.

Acta Hepatogastroenterol (Stuttg) 1978 Apr;25(2):139-43

Antibodies were readily produced in three rabbits to unconjugated pure natural porcine secretin, and in two rabbits to synthetic porcine secretin conjugated to BSA. The final dilutions of the antisera to bind 50% of 1 fmol 125-i-labeled secretin prepared by the Chloramine-T method and purified on a Sephadex G-15 and a SP Sephadex C-25 column varied between 1:14,500 and 1:245,000. The effective equilibrium constants (Keff) according to Scatchard varied between 0. Read More

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Guinea-pig milk-protein synthesis. Isolation and characterization of messenger ribonucleic acids from lactating mammary gland and identification of caseins and pre-alpha-lactalbumin as translation products in heterologous cell-free systems.

Biochem J 1976 Oct;160(1):57-74

1. The major milk proteins synthesized by the lactating mammary gland of the guinea pig were identified and designated as caseins A, B and C and alpha-lactalbumin, with estimated mol.wts. Read More

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October 1976

Instrumental assay of microbial lipase at constant pH.

Appl Microbiol 1967 Jan;15(1):110-3

A rapid, accurate method with high sensitivity and reproducibility, and having the advantage of a short incubation period under constant pH, has been developed for routine measurement of microbial lipase. Assembled from readily available and economical instrumental components, the apparatus includes a pH meter, a thermoelectric heating and stirring device, a motor-driven burette, and an automatic recorder. The reaction mixture, consisting of 5 ml of a 10% olive oil-gum arabic emulsion, 2 ml of 3 m NaCl, 2 ml of sodium taurocholate (15 mg/ml) of 0. Read More

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January 1967
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