6,119 results match your criteria probe lead


Feasibility of longitudinal monitoring of atherosclerosis with Pulse Wave Imaging in a swine model.

Physiol Meas 2021 Sep 22. Epub 2021 Sep 22.

Department of Biomedical Engineering, Columbia University, MC 8904, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA, New York, New York, UNITED STATES.

Objective: Atherosclerosis is a vascular disease characterized by compositional and mechanical changes in the arterial walls that lead to a plaque buildup. Depending on its geometry and composition, a plaque can ruptured and cause stroke, ischemia or infarction. Pulse Wave Imaging (PWI) is an ultrasound-based technique developed to locally quantify the stiffness of arteries. Read More

View Article and Full-Text PDF
September 2021

Sacral Neuromodulation Changes Pelvic Floor Activity in Overactive Bladder Patients-Possible New Insights in Mechanism of Action: A Pilot Study.

Neuromodulation 2021 Sep 21. Epub 2021 Sep 21.

Department of Urology, Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.

Objectives: To evaluate if electrodiagnostic tools can advance the understanding in the effect of sacral neuromodulation (SNM) on pelvic floor activity, more specifically if SNM induces changes in pelvic floor muscle (PFM) contraction.

Materials And Methods: Single tertiary center, prospective study (October 2017-May 2018) including patients with overactive bladder syndrome undergoing SNM. Electromyography of the PFM was recorded using the Multiple Array Probe Leiden. Read More

View Article and Full-Text PDF
September 2021

A non-peptide probe for detecting chymotrypsin activity based on protection-deprotection strategy in living systems.

J Mater Chem B 2021 Sep 21. Epub 2021 Sep 21.

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P. R. China.

Chymotrypsin (CHT) plays a vital role in the metabolism of organisms and affects cell proliferation and apoptosis. Abnormal levels of CHT will lead to a variety of diseases, such as inflammatory arthritis, diabetes, pharyngitis, indigestion, and pancreatic cancer. Therefore, it is significant to design an effective method for the detection of CHT in living systems. Read More

View Article and Full-Text PDF
September 2021

High behavioural variability mediated by altered neuronal excitability in mutant zebrafish.

eNeuro 2021 Sep 17. Epub 2021 Sep 17.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065 India

Autism spectrum disorders (ASDs) are characterized by abnormal behavioral traits arising from neural circuit dysfunction. While a number of genes have been implicated in ASDs, in most cases, a clear understanding of how mutations in these genes lead to circuit dysfunction and behavioral abnormality is absent. The () gene is one such gene, associated with ASDs, intellectual disability and a range of other neurodevelopmental conditions. Read More

View Article and Full-Text PDF
September 2021

DNA dendrimer-templated copper nanoparticles: self-assembly, aggregation-induced emission enhancement and sensing of lead ions.

Mikrochim Acta 2021 09 19;188(10):346. Epub 2021 Sep 19.

State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.

Copper nanomaterials based on DNA scaffold (DNA-Cu NMs) are becoming a novel fluorescent material, but it is still challenging to obtain highly fluorescent DNA-Cu NMs with excellent stability. In this work, we report a kind of copper nano-assemblies (Cu NASs) with aggregation-induced emission enhancement (AIEE) property using DNA dendrimers with sticky end as template. The sticky end of the DNA dendrimers induced the formation of much bigger Cu NASs with average size ranging from 131 to 264 nm, depending on the length of the DNA dendrimer sticky end from 6 bases to 27 bases. Read More

View Article and Full-Text PDF
September 2021

Protein and solutes freeze-concentration in water/glycerol mixtures revealed by pulse EPR.

Eur J Pharm Biopharm 2021 Sep 14. Epub 2021 Sep 14.

Fachbereich Physik, Universität Osnabrück, 49069 Osnabrück, Germany.

Lyophilization can extend protein drugs stability and shelf life, but it also can lead to protein degradation in some cases. The development of safe freeze-drying approaches for sensitive proteins requires a better understanding of lyophilization on the molecular level. The evaluation of the freezing process and its impact on the protein environment in the nm scale is challenging because feasible experimental methods are scarce. Read More

View Article and Full-Text PDF
September 2021

Image-guided EBUS bronchoscopy system for lung-cancer staging.

Inform Med Unlocked 2021 13;25. Epub 2021 Jul 13.

School of Electrical Engineering and Computer Science, Penn State University, University Park, PA, United States.

The staging of the central-chest lymph nodes is a major step in the management of lung-cancer patients. For this purpose, the physician uses a device that integrates videobronchoscopy and an endobronchial ultrasound (EBUS) probe. To biopsy a lymph node, the physician first uses videobronchoscopy to navigate through the airways and then invokes EBUS to localize and biopsy the node. Read More

View Article and Full-Text PDF

Click-to-lead design of a picomolar ABA receptor antagonist with potent activity in vivo.

Proc Natl Acad Sci U S A 2021 Sep;118(38)

Institute for Integrative Genome Biology, University of California, Riverside, CA 92521;

Abscisic acid (ABA) is a key plant hormone that mediates both plant biotic and abiotic stress responses and many other developmental processes. ABA receptor antagonists are useful for dissecting and manipulating ABA's physiological roles in vivo. We set out to design antagonists that block receptor-PP2C interactions by modifying the agonist opabactin (OP), a synthetically accessible, high-affinity scaffold. Read More

View Article and Full-Text PDF
September 2021

Radial spatiotemporal evolution of rf magnetic field and plasma current in a very-high-frequency plasma source over a wide range of plasma density and pressure.

Phys Rev E 2021 Aug;104(2-2):025207

Tokyo Electron Technology Solutions Limited, 2381-1 Kitagejo, Fujii-cho, Nirasaki City, Yamanashi 407-8511, Japan.

We present detailed spatiotemporal measurements of rf magnetic fields and plasma conduction current as well as the corresponding plasma density profiles when very-high-frequency 100-MHz waves are introduced to a parallel plate plasma. The magnetic probe, optical emission, and plasma absorption probe measurements support a model in which the interaction of high-frequency waves with the moving plasma sheath at specific phases of the very high frequencies (VHF) period leads to local reversals in the vertical rf electric field which can lead to conduction current reversals and circulations. The presence of current reversals and circulation correlate with measured plasma uniformity. Read More

View Article and Full-Text PDF

Lead removal from synthetic wastewater by biosorbents prepared from seeds of Artocarpus Heterophyllus and Syzygium Cumini.

Chemosphere 2021 Aug 27;287(Pt 1):132016. Epub 2021 Aug 27.

Department of Chemical Engineering, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India. Electronic address:

The present investigation deals with removal of lead (Pb) ions from waste water using biosorbent prepared from seeds of Artocarpus heterophyllus (SBAh) and Syzygium cumini (SBSc). Biosorbents surface has been characterized through FT-IR spectroscopy to probe the presence of functional groups. Response surface methodology enabled optimized conditions (Pb concentration 2 μg/mL, pH 5. Read More

View Article and Full-Text PDF

Sulphide activity-dependent multicolor emission dye and its applications in imaging.

Analyst 2021 Sep 13;146(18):5517-5527. Epub 2021 Sep 13.

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.

Reactive sulfur species (RSS) play pivotal roles in various pathological and physiological processes. There exists an intricate relevance in generation and metabolism among these substances. Although they are nucleophilic, there are still some differences in their reactivity. Read More

View Article and Full-Text PDF
September 2021

Target-receptive structural switching of ssDNA as selective and sensitive biosensor for subsequent detection of toxic Pb and organophosphorus pesticide.

Chemosphere 2021 Sep 6;287(Pt 2):132163. Epub 2021 Sep 6.

Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Chennai, 603 110, India; Centre of Excellence in Water Research (CEWAR), Sri Sivasubramaniya Nadar College of Engineering, Chennai, 603 110, India. Electronic address:

A structural switching in Single-stranded DNA (ssDNA) fluorescence biosensor for quick turn-on/off detection of Pb ions and pesticide was reported. The design strategy of Hex-labelled ssDNA consists of two types of aptamer probe, G-rich base pair sequence forms G-quadruplex confirmation with Pb ions. While other part of base pair sequence exhibits affinity to fold isocarbophos pesticide. Read More

View Article and Full-Text PDF
September 2021

[Comparison of the performance of secretome analysis based on metabolic labeling by three unnatural sugars].

Se Pu 2021 Oct;39(10):1086-1093

Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen 518055, China.

Many secreted proteins, including cytokines, growth factors and hormones, are crucial in processes like intercellular signaling. Dynamic changes in secreted proteins usually reflect the growth and pathological state of the cells. Many drug targets are secretory proteins. Read More

View Article and Full-Text PDF
October 2021

Anti-Inflammatory CeO Nanoparticles Prevented Cytotoxicity Due to Exogenous Nitric Oxide Donors via Induction Rather Than Inhibition of Superoxide/Nitric Oxide in HUVE Cells.

Molecules 2021 Sep 6;26(17). Epub 2021 Sep 6.

Department of Physics and Astronomy, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia.

The mechanism behind the cytoprotective potential of cerium oxide nanoparticles (CeO NPs) against cytotoxic nitric oxide (NO) donors and HO is still not clear. Synthesized and characterized CeO NPs significantly ameliorated the lipopolysaccharide (LPS)-induced cytokines IL-1β and TNF-α. The main goal of this study was to determine the capacities of NPs regarding signaling effects that could have occurred due to reactive oxygen species (ROS) and/or NO, since NP-induced ROS/NO did not lead to toxicity in HUVE cells. Read More

View Article and Full-Text PDF
September 2021

Signal amplification of SiO nanoparticle loaded horseradish peroxidase for colorimetric detection of lead ions in water.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 30;265:120342. Epub 2021 Aug 30.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China. Electronic address:

In this work, we developed an aptamer-based optical assay for the analysis of Pb, a hazardous heavy metal that may be present in the food chain and harmful to human health. An aptamer targeted against Pb was immobilized onto the microplate as the capture probe. SiO nanoparticles (NPs) were synthesized and used as carriers of the signaling horseradish peroxidase (HRP) to achieve amplification of the optical signal. Read More

View Article and Full-Text PDF

Advancing Plasmon-Induced Selectivity in Chemical Transformations with Optically Coupled Transmission Electron Microscopy.

Acc Chem Res 2021 Sep 7. Epub 2021 Sep 7.

Department of Material Science and Engineering, Stanford University School of Engineering, Stanford, California 94305, United States.

ConspectusNanoparticle photocatalysts are essential to processes ranging from chemical production and water purification to air filtration and surgical instrument sterilization. Photochemical reactions are generally mediated by the illumination of metallic and/or semiconducting nanomaterials, which provide the necessary optical absorption, electronic band structure, and surface faceting to drive molecular reactions. However, with reaction efficiency and selectivity dictated by atomic and molecular interactions, imaging and controlling photochemistry at the atomic scale are necessary to both understand reaction mechanisms and to improve nanomaterials for next-generation catalysts. Read More

View Article and Full-Text PDF
September 2021

Transcriptional and Post-Transcriptional Polar Effects in Bacterial Gene Deletion Libraries.

mSystems 2021 Sep 7:e0081321. Epub 2021 Sep 7.

European Molecular Biology Laboratorygrid.4709.a, Genome Biology Unit, Heidelberg, Germany.

Single-gene deletions can affect the expression levels of other genes in the same operon in bacterial genomes. Here, we used proteomics for 133 Escherichia coli gene deletion mutants and transcriptome sequencing (RNA-seq) data from 71 mutants to probe the extent of transcriptional and post-transcriptional effects of gene deletions in operons. Transcriptional effects were common on genes located downstream of the deletion and were consistent across all operon members, with nearly 40% of operons showing greater than 2-fold up- or downregulation. Read More

View Article and Full-Text PDF
September 2021

Further hit optimization of 6-(trifluoromethyl)pyrimidin-2-amine based TLR8 modulators: Synthesis, biological evaluation and structure-activity relationships.

Eur J Med Chem 2021 Aug 28;225:113809. Epub 2021 Aug 28.

Chair of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ljubljana, Aškerčeva c. 7, SI-1000, Ljubljana, Slovenia. Electronic address:

Toll-like receptor 8 (TLR8) is an endosomal TLR that has an important role in the innate human immune system, which is involved in numerous pathological conditions. Excessive activation of TLR8 can lead to inflammatory and autoimmune diseases, which highlights the need for development of TLR8 modulators. However, only a few small-molecule modulators that selectively target TLR8 have been developed. Read More

View Article and Full-Text PDF

Multipulse Terahertz Spectroscopy Unveils Hot Polaron Photoconductivity Dynamics in Metal-Halide Perovskites.

J Phys Chem Lett 2021 Sep 3;12(36):8732-8739. Epub 2021 Sep 3.

Department of Chemistry and Centre for Processable Electronics, Imperial College London, London W12 0BZ, United Kingdom.

Hot carriers in metal-halide perovskites (MHPs) present a foundation for understanding carrier-phonon coupling in the materials as well as the prospective development of high-performance hot carrier photovoltaics. While the carrier population dynamics during cooling have been scrutinized, the evolution of the hot carrier properties, namely mobility, remains largely unexplored. Here we introduce novel ultrafast visible pump-infrared push-terahertz probe spectroscopy to monitor the real-time conductivity dynamics of cooling carriers in methylammonium lead iodide. Read More

View Article and Full-Text PDF
September 2021

Paradoxical facilitation alongside interhemispheric inhibition.

Exp Brain Res 2021 Sep 2. Epub 2021 Sep 2.

Bloorview Research Institute, Holland Bloorview Kids Rehabilitation Hospital, Toronto, ON, Canada.

Neurophysiological experiments using transcranial magnetic stimulation (TMS) have sought to probe the function of the motor division of the corpus callosum. Primary motor cortex sends projections via the corpus callosum with a net inhibitory influence on the homologous region of the opposite hemisphere. Interhemispheric inhibition (IHI) experiments probe this inhibitory pathway. Read More

View Article and Full-Text PDF
September 2021

Revealing Ultrafast Charge-Carrier Thermalization in Tin-Iodide Perovskites through Novel Pump-Push-Probe Terahertz Spectroscopy.

ACS Photonics 2021 Aug 9;8(8):2509-2518. Epub 2021 Aug 9.

Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford OX1 3PU, U.K.

Tin-iodide perovskites are an important group of semiconductors for photovoltaic applications, promising higher intrinsic charge-carrier mobilities and lower toxicity than their lead-based counterparts. Controllable tin vacancy formation and the ensuing hole doping provide interesting opportunities to investigate dynamic intraband transitions of charge carriers in these materials. Here, we present for the first time an experimental implementation of a novel Optical-Pump-IR-Push-THz-Probe spectroscopic technique and demonstrate its suitability to investigate the intraband relaxation dynamics of charge carriers brought into nonequilibrium by an infrared "push" pulse. Read More

View Article and Full-Text PDF

The homogenous alternative to biomineralization: Zn- and Mn-rich materials enable sharp organismal "tools" that reduce force requirements.

Sci Rep 2021 Sep 1;11(1):17481. Epub 2021 Sep 1.

Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR, 97331, USA.

We measured hardness, modulus of elasticity, and, for the first time, loss tangent, energy of fracture, abrasion resistance, and impact resistance of zinc- and manganese-enriched materials from fangs, stings and other "tools" of an ant, spider, scorpion and nereid worm. The mechanical properties of the Zn- and Mn-materials tended to cluster together between plain and biomineralized "tool" materials, with the hardness reaching, and most abrasion resistance values exceeding, those of calcified salmon teeth and crab claws. Atom probe tomography indicated that Zn was distributed homogeneously on a nanometer scale and likely bound as individual atoms to more than ¼ of the protein residues in ant mandibular teeth. Read More

View Article and Full-Text PDF
September 2021

Effect of Surfactant Concentration and Hydrophobicity on the Ordering of Water at a Silica Surface.

Langmuir 2021 Sep 29;37(36):10806-10817. Epub 2021 Aug 29.

Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.

The performance of nonionic surfactants is mediated by the interfacial interactions at the solid-liquid interface. Here we applied sum frequency generation (SFG) vibrational spectroscopy to probe the molecular structure of the silica-nonionic surfactant solution interface in situ, supplemented by quartz crystal microbalance with dissipation monitoring (QCM-D) and molecular dynamics (MD) simulations. The combined studies elucidated the effects of nonionic surfactant solution concentration, surfactant composition, and rinsing on the silica-surfactant solution interfacial structure. Read More

View Article and Full-Text PDF
September 2021

Comparing the Sensitivity of a Low Frequency Versus a High Frequency Probe in the Detection of Pneumothorax in a Swine Model.

Med J (Ft Sam Houst Tex) 2021 Jul-Sep(PB 8-21-07/08/09):13-19

Baylor University.

Background: Correct diagnosis of pneumothorax in trauma patients is essential. Under-diagnosis can lead to development of life-threatening tension pneumothorax, but overdiagnosis leads to placement of unnecessary chest tubes with potential related morbidity and pain. It is unclear from previous work if there is a benefit to switching from the phased array (low frequency) probe to the linear (high frequency) probe. Read More

View Article and Full-Text PDF

Temperature Effects on Optical Trapping Stability.

Micromachines (Basel) 2021 Aug 12;12(8). Epub 2021 Aug 12.

Nanomaterials for Bioimaging Group, Departamento de Física de Materiales, Universidad Autónoma de Madrid, Calle Francisco Tomás y Valiente, 7, 28048 Madrid, Spain.

In recent years, optically trapped luminescent particles have emerged as a reliable probe for contactless thermal sensing because of the dependence of their luminescence on environmental conditions. Although the temperature effect in the optical trapping stability has not always been the object of study, the optical trapping of micro/nanoparticles above room temperature is hindered by disturbances caused by temperature increments of even a few degrees in the Brownian motion that may lead to the release of the particle from the trap. In this report, we summarize recent experimental results on thermal sensing experiments in which micro/nanoparticles are used as probes with the aim of providing the contemporary state of the art about temperature effects in the stability of potential trapping processes. Read More

View Article and Full-Text PDF

Deciphering Microbial Metal Toxicity Responses using RB-TnSeq and Activity-Based Metabolomics.

Appl Environ Microbiol 2021 Aug 25:AEM0103721. Epub 2021 Aug 25.

Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 30602.

To uncover metal toxicity targets and defense mechanisms of the facultative anaerobe strain sp. MT58 (MT58), we used a multi-omic strategy combining two global techniques, random bar code transposon-site sequencing (RB-TnSeq) and activity-based metabolomics. MT58 is a metal-tolerant Oak Ridge Reservation (ORR) environmental isolate that was enriched in the presence of metals at concentrations measured in contaminated groundwater at an ORR nuclear waste site. Read More

View Article and Full-Text PDF

Development of Novel PCR Assays for Improved Detection of Enterovirus D68.

J Clin Microbiol 2021 Aug 25:JCM0115121. Epub 2021 Aug 25.

Department of Pediatrics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

Enterovirus D68 (EV-D68) causes a range of clinical manifestations, including asthma-like illness, severe respiratory disease, and acute flaccid myelitis. EV-D68 has caused worldwide outbreaks since 2014 and is now recognized as a re-emerging infection in many countries. EV-D68-specific PCR assays are widely used for the diagnosis of EV-D68 infection; however, assay sensitivity is a concern because of genetic changes in recently circulated EV-D68. Read More

View Article and Full-Text PDF

Microstructural, microchemical, and mechanical changes associated with the clinical reuse of two nickel-titanium endodontic instruments.

Dent Res J (Isfahan) 2021 22;18:48. Epub 2021 Jun 22.

Department of Integrated Basic Studies, Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Medellin, Colombia.

Background: Nickel-titanium (NiTi) instruments have represented a great technological development that enabled endodontists conforming irregular-shaped root canals. Notwithstanding, the repeated use of these instruments may lead to the fracture without any prior visible warning signs. This study aimed to evaluate how multiple clinical instrumentation/sterilization cycles of two NiTi mechanized instruments can affect their microstructural, microchemical, and mechanical characteristics. Read More

View Article and Full-Text PDF

A Background Assessable and Correctable Bimolecular Fluorescence Complementation System for Nanoscopic Single-Molecule Imaging of Intracellular Protein-Protein Interactions.

ACS Nano 2021 Aug 24. Epub 2021 Aug 24.

Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing 100871, China.

Bimolecular Fluorescence Complementation (BiFC) is a versatile approach for intracellular analysis of protein-protein interactions (PPIs), but the tendency of the split fluorescent protein (FP) fragments to self-assemble when brought into close proximity of each other by random collision can lead to generation of false-positive signals that hamper high-definition imaging of PPIs occurring on the nanoscopic level. While it is thought that expressing the fusion proteins at a low level can remove false positives without impacting specific signals, there has been no effective strategy to test this possibility. Here, we present a system capable of assessing and removing BiFC false positives, termed Background Assessable and Correctable-BiFC (BAC-BiFC), in which one of the split FP fragments is fused with an optically distinct FP that serves as a reference marker, and the single-cell fluorescence ratio of the BiFC signal to the reference signal is used to gauge an optimal transfection condition. Read More

View Article and Full-Text PDF

Energy Tuning of Electronic Spin Coherent Evolution in Methylammonium Lead Iodide Perovskites.

J Phys Chem Lett 2021 Sep 23;12(34):8272-8279. Epub 2021 Aug 23.

CNRS, Institut des NanoSciences de Paris, Sorbonne Université, 4 place Jussieu, F-75005 Paris, France.

We investigated the coherent evolution of the electronic spin at low temperature in high-quality CHNHPbI polycrystalline films by picosecond-resolved photoinduced Faraday rotation. We show that this coherent evolution can be tuned by choosing the pump-probe energy within the lowest optical-absorption band, and we explain it as the result of two main contributions: the localized electron and the localized hole. Their corresponding amplitude ratios are constant across the lowest absorption band-an observation which disqualifies a free exciton from being at the origin of the electronic spin coherent evolution. Read More

View Article and Full-Text PDF
September 2021