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Insight into sulfur dioxide and its derivatives metabolism in living system with visualized evidences via ultra-sensitive fluorescent probe.

J Hazard Mater 2021 Sep 11;423(Pt B):127179. Epub 2021 Sep 11.

CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China; School of Pharmacy, Binzhou Medical University, Yantai 264003, China. Electronic address:

Sulfur dioxide (SO) and its derivatives have long been considered as hazardous environmental pollutants but commonly used as food additives in safe dose range. They also could be produced from biological metabolism process of sulfur-containing amino acids. However, their physiological roles remain extremely obscure mainly due to lack of efficient tools for monitoring and imaging strategy establishment. Read More

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September 2021

Combination of high specific activity carbon-14 labeling and high resolution mass spectrometry to study pesticide metabolism in crops: Metabolism of cycloxaprid in rice.

Environ Int 2021 Sep 17;157:106879. Epub 2021 Sep 17.

Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.

The study of pesticide metabolism in crops is critical for assessing the mode of action and environmental risks of pesticides. However, the study of pesticide metabolism in crops is usually complicated and it is often a daunting challenge to accurately screen the metabolites of novel pesticides in complex matrices. This study demonstrated a combined use of high-specific activity carbon-14 labeling and high-resolution mass spectrometry (HSA-C-HRMS) for metabolism profiling of a novel neonicotinoid cycloxaprid in rice. Read More

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September 2021

Label-free and reagentless electrochemical genosensor based on graphene acid for meat adulteration detection.

Biosens Bioelectron 2021 Sep 12;195:113628. Epub 2021 Sep 12.

Catalan Institute of Nanoscience and Nanotechnology, Autonomous University of Barcelona, 08193, Bellaterra, Barcelona, Spain. Electronic address:

With the increased demand for beef in emerging markets, the development of quality-control diagnostics that are fast, cheap and easy to handle is essential. Especially where beef must be free from pork residues, due to religious, cultural or allergic reasons, the availability of such diagnostic tools is crucial. In this work, we report a label-free impedimetric genosensor for the sensitive detection of pork residues in meat, by leveraging the biosensing capabilities of graphene acid - a densely and selectively functionalized graphene derivative. Read More

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September 2021

MXene-based cytosensor for the detection of HER2-positive cancer cells using [email protected] magnetic nanohybrids conjugated to the HB5 aptamer.

Biosens Bioelectron 2021 Sep 12;195:113626. Epub 2021 Sep 12.

Department of Mechanical Engineering, Australian College of Kuwait, Mishref, Safat 13015, Kuwait; Centre for Infrastructure Engineering, Western Sydney University, Penrith 2751, NSW, Australia.

MXenes are a new class of conductive two-dimensional material which have received growing attention in biosensing for their significant surface area and unique surface chemistry. Here, gold electrodes were modified with MXene nanosheets of about 2 nm thickness and 1.5 μm lateral size for the electrochemical detection of tumor cells. Read More

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September 2021

Characterizing Cation Chemistry for Anion Exchange Membranes--Alkyl-Substituted Benzyltetramethylimidazolium and Benzyltrimethylammonium Salts in Base.

J Org Chem 2021 Sep 20. Epub 2021 Sep 20.

Corporate Research Laboratories 3M Company 3M Center, Saint Paul, Minnesota 55144, United States.

Base stability and degradation of imidazolium-functional materials have been a focus of research on anion exchange membranes for electrochemical devices. Despite extensive work, mechanistic questions can cloud efforts to correlate results from model compounds and polymeric membranes. Here, we report an NMR study of behavior in aqueous KOH of benzyltetramethylimidazolium and benzyltrimethylammonium salts with and without -alkyl-substitution to probe whether base attack at the alkyl substituent plays a role in the reaction pathways. Read More

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September 2021

Photoreaction of DNA Containing 5-Halouracil and its Products.

Photochem Photobiol 2021 Sep 20. Epub 2021 Sep 20.

Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto, 606-8502, Japan.

5-Halouracil, which is a DNA base analog in which the methyl group at the C5 position of thymine is replaced with a halogen atom, has been used in studies of DNA damage. In DNA strands, the uracil radical generated from 5-halouracil causes DNA damage via a hydrogen-abstraction reaction. We analyzed the photoreaction of 5-halouracil in various DNA structures and revealed that the reaction is DNA-structure dependent. Read More

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September 2021

On-Strand Knoevenagel Insertion of a Hemicyanine Molecular Rotor Loop Residue for Turn-On Fluorescence Detection of Pb-Induced G-Quadruplex Rigidity.

Bioconjug Chem 2021 Sep 20. Epub 2021 Sep 20.

Departments of Chemistry and Toxicology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

We demonstrate the ability to distinguish Pb from K within the central cavity of the antiparallel G-quadruplex (GQ) DNA produced by the thrombin binding aptamer (TBA) using an internal molecular rotor fluorescent probe. An indole-aldehyde containing an acyclic -glycol group was first employed in the on-strand Knoevenagel condensation with five different heterocyclic quaternary cationic acceptors to assess the molecular rotor character of the resulting cyanine-styryl dyes within duplex DNA. An indole-pyridinium (4PI) nucleobase surrogate displayed the greatest turn-on emission response to duplex formation and was thus inserted into the loop residues of TBA to monitor GQ-folding in the presence of Pb versus K. Read More

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September 2021

Design and Development of a Chemical Probe for Pseudokinase Ca/calmodulin-Dependent Ser/Thr Kinase.

J Med Chem 2021 Sep 20. Epub 2021 Sep 20.

Structural Genomics Consortium (SGC), Buchmann Institute for Life Sciences (BMLS), Goethe University Frankfurt am Main, Max-von-Laue-Str. 15, Frankfurt am Main 60438, Germany.

CASK (Ca/calmodulin-dependent Ser/Thr kinase) is a member of the MAGUK (membrane-associated guanylate kinase) family that functions as neurexin kinases with roles implicated in neuronal synapses and trafficking. The lack of a canonical DFG motif, which is altered to GFG in CASK, led to the classification as a pseudokinase. However, functional studies revealed that CASK can still phosphorylate substrates in the absence of divalent metals. Read More

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September 2021

Correlation Analysis of Pleural Effusion and Lung Infection After Liver Transplantation.

Clin Lab 2021 Sep;67(9)

Background: The aim was to probe the association of pleural effusion with lung infection in patients with liver transplantation and to provide a theoretical foundation for preventing, diagnosing, and remedying pulmonary complications after liver transplantation.

Methods: Our team harvested clinical data of patients undergoing orthotopic allogeneic liver transplantation complicated with pleural effusion after surgery in our institution from May 2018 to July 2019. Based on whether puncture drainage was needed, patients were allocated to either control group or observation group. Read More

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September 2021

The Finding My Way UK Clinical Trial: Adaptation Report and Protocol for a Replication Randomized Controlled Efficacy Trial of a Web-Based Psychological Program to Support Cancer Survivors.

JMIR Res Protoc 2021 Sep 20;10(9):e31976. Epub 2021 Sep 20.

College of Education, Psychology & Social Work, Flinders University, Adelaide, Australia.

Background: Cancer survivors frequently report a range of unmet psychological and supportive care needs; these often continue after treatment has finished and are predictive of psychological distress and poor health-related quality of life. Web-based interventions demonstrate good efficacy in addressing these concerns and are more accessible than face-to-face interventions. Finding My Way (FMW) is a web-based, psycho-educational, and cognitive behavioral therapy intervention for cancer survivors developed in Australia. Read More

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September 2021

Sticking Effect of a Tackifier on the Fibrillation of Acrylic Pressure-Sensitive Adhesives.

Langmuir 2021 Sep 20. Epub 2021 Sep 20.

LINTEC Corporation, 5-14-42 Nishiki-cho, Warabi-shi, Saitama 335-0005, Japan.

In this study, the effect of a tackifier on the viscoelastic and adhesion properties of acrylic pressure-sensitive adhesives (PSAs) was investigated. The intermediate products in the process of PSA synthesis, including an acrylate-based copolymer solution, a cross-linked copolymer, and the final product with a tackifier, were prepared and characterized using dynamic mechanical analysis (DMA). A significant increase in storage and loss moduli at high angular velocities was observed for the final product with the tackifier. Read More

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September 2021

A MOF-Shell-Confined I-Motif-Based pH Probe (MOFC-i) Strategy for Sensitive and Dynamic Imaging of Cell Surface pH.

ACS Appl Mater Interfaces 2021 Sep 20. Epub 2021 Sep 20.

School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China.

Dynamic imaging of cell surface pH is extremely challenging due to the slight changes in pH and the fast diffusion of secreted acid to the extracellular environment. In this work, we construct a novel metal-organic framework (MOF)-shell-confined i-motif-based pH probe (MOFC-i) strategy that enables sensitive and dynamic imaging of cell surface pH. The CY3- and CY5-labeled i-motif, which is hybridized via its short complementary chain with two-base mismatches, is optimized for sensing at physiological pH. Read More

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September 2021

Detection of organophosphorus pesticides: exploring oxime as a probe with improved sensitivity by CeO-modified electrode.

Anal Methods 2021 Sep 20. Epub 2021 Sep 20.

MEMS Center, Harbin Institute of Technology, Harbin 150001, China.

In this paper, the catalytic activity of CeO NPs toward oxime oxidation was adopted for the first time to develop an electrochemical sensor with improved sensitivity toward the direct detection of organophosphorus pesticides (OPs) without electrochemical redox activity. To enhance the conductivity of the sensor, CeO NPs together with multi-walled carbon nanotubes (MWCNTs) were deposited onto a bare glassy carbon electrode (GCE) by the simple method of drop-casting. The electrochemical properties of the as-prepared sensor were evaluated in K[Fe(CN)] solution and the oxidation behavior of pralidoxime (PAM) chloride on the electrodes was characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Read More

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September 2021

Loneliness and the Social Brain: How Perceived Social Isolation Impairs Human Interactions.

Adv Sci (Weinh) 2021 Sep 20:e2102076. Epub 2021 Sep 20.

Division of Medical Psychology, Department of Psychiatry and Psychotherapy, University Hospital Bonn, 53105, Bonn, Germany.

Loneliness is a painful condition associated with increased risk for premature mortality. The formation of new, positive social relationships can alleviate feelings of loneliness, but requires rapid trustworthiness decisions during initial encounters and it is still unclear how loneliness hinders interpersonal trust. Here, a multimodal approach including behavioral, psychophysiological, hormonal, and neuroimaging measurements is used to probe a trust-based mechanism underlying impaired social interactions in loneliness. Read More

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September 2021

Boron-Mediated Grain Boundary Engineering Enables Simultaneous Improvement of Thermoelectric and Mechanical Properties in N-Type Bi Te.

Small 2021 Sep 19:e2104067. Epub 2021 Sep 19.

Institute of Physics IA, RWTH Aachen University, 52056, Aachen, Germany.

Powder metallurgy introduces small structures of high-density grain boundaries into Bi Te -based alloys, which promises to enhance their mechanical and thermoelectric performance. However, due to the strong donor-like effect induced by the increased surface, Te vacancies form in the powder-metallurgy process. Hence, the as-sintered n-type Bi Te -based alloys show a lower figure of merit (ZT) value than their p-type counterparts and the commercial zone-melted (ZM) ingots. Read More

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September 2021

Genotyping of human papillomavirus DNA in Wielkopolska region.

Ginekol Pol 2021 Sep 20. Epub 2021 Sep 20.

Department of Gynaecological Endocrinology and Gynaecology, Jagiellonian University Medical College, Cracow, Poland.

Objectives: Human papillomavirus infection is one of the most common sexually transmitted diseases. Long-term exposure to the HPV leads to development of high-grade squamous intraepithelial lesions that can eventually transform into cervical cancer. The aim of the study was to assess the HPV genotype distribution in patients with abnormal pap smear and provide prospective study. Read More

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September 2021

Functional Neural Correlates of a Useful Field of View (UFOV)-Based fMRI Task in Older Adults.

Cereb Cortex 2021 Sep 20. Epub 2021 Sep 20.

Center for Cognitive Aging and Memory, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.

Declines in processing speed performance occur in aging and are a critical marker of functional independence in older adults. Studies suggest that Useful Field of View (UFOV) training may ameliorate cognitive decline. Despite its efficacy, little is known about the neural correlates of this task. Read More

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September 2021

Detection of Protein -nitrosothiols (SNOs) in Plant Samples on Diaminofluorescein (DAF) Gels.

Bio Protoc 2017 Sep 20;7(18):e2559. Epub 2017 Sep 20.

Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Spanish National Research Council (CSIC), C/Profesor Albareda, Granada, Spain.

In plant cells, the analysis of protein -nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems ( King , 2005 ) for plant samples. Briefly, proteins from plant samples are separated via non-reducing SDS-PAGE, then the NO bound by -nitrosylated proteins is released using UV light and, finally, the NO is detected using the fluorescent probe DAF-FM (Rodriguez-Ruiz , 2017). Read More

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September 2017

Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2',7'-Dichlorodihydrofluorescein Diacetate (DCFH-DA).

Bio Protoc 2017 Sep 5;7(17):e2545. Epub 2017 Sep 5.

Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, India.

Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells that become toxic for the cell survival. Here we present the method for detection of ROS by using an oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in cyanobacteria. Read More

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September 2017

Staining of Membrane Receptors with Fluorescently-labeled DNA Aptamers for Super-resolution Imaging.

Bio Protoc 2017 Sep 5;7(17):e2541. Epub 2017 Sep 5.

Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.

One of the most prominent applications of fluorescent super-resolution microscopy is the study of nanodomain arrangements of receptors and the endocytic pathway. Staining methods are becoming crucial for answering questions on the nanoscale, therefore, the use of small and monovalent affinity probes is of great interest in super-resolution microscopy with biological samples. One kind of affinity probe is the aptamer. Read More

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September 2017

ROS Detection in Colonies with CellROX Green Reagent.

Bio Protoc 2017 Aug 20;7(16):e2508. Epub 2017 Aug 20.

Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.

We analyzed the reactive oxygen species (ROS) accumulation in the colony-forming green microalga in response to several stress inducers such as NaCl, NaHCO, salicylic acid (SA), methyl jasmonate, and acetic acid. A staining assay using the fluorescent dye CellROX Green was used. CellROX Green is a fluorogenic probe used for measuring oxidative stress in live cells. Read More

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Fluorescently Labelled Aerolysin (FLAER) Labelling of Cells.

Bio Protoc 2017 Jun 5;7(11):e2303. Epub 2017 Jun 5.

School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.

In this protocol we describe a nonradiolabelled labelling of GPI anchor in The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be measured. The labelling does not need permeabilization of cells and can be carried out . Read More

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Semi-quantitative Analysis of H4K20me1 Levels in Living Cells Using Mintbody.

Bio Protoc 2017 May 20;7(10):e2276. Epub 2017 May 20.

Cell Biology Unit, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan.

Eukaryotic nuclear DNA wraps around histone proteins to form a nucleosome, a basic unit of chromatin. Posttranslational modification of histones plays an important role in gene regulation and chromosome duplication. Some modifications are quite stable to be an epigenetic memory, and others exhibit rapid turnover or fluctuate during the cell cycle. Read More

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3D-printed Recoverable Microdrive and Base Plate System for Rodent Electrophysiology.

Bio Protoc 2021 Aug 20;11(16):e4137. Epub 2021 Aug 20.

Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USA.

Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (, µLED and Neuropixels silicon probes), implantation methods for silicon probes in rats and mice have not advanced substantially for a decade. The surgery is complex, takes time to master, and involves handling expensive devices and valuable animal subjects. Read More

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A Novel Method to Map Small RNAs with High Resolution.

Bio Protoc 2021 Aug 20;11(16):e4128. Epub 2021 Aug 20.

Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA.

Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics . However, the application of super-resolution imaging techniques to detect small RNAs (sRNAs) is limited by the choice of proper fluorophores, autofluorescence of samples, and failure to multiplex. Read More

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Application of Genetic Algorithm-Based Support Vector Machine in Identification of Gene Expression Signatures for Psoriasis Classification: A Hybrid Model.

Biomed Res Int 2021 8;2021:5520710. Epub 2021 Sep 8.

Department of Dermatology, Psoriasis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: Psoriasis is a chronic autoimmune disease impairing significantly the quality of life of the patient. The diagnosis of the disease is done via a visual inspection of the lesional skin by dermatologists. Classification of psoriasis using gene expression is an important issue for the early and effective treatment of the disease. Read More

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September 2021

Single Multiple Cross Displacement Amplification for Rapid and Real-Time Detection of Porcine Circovirus 3.

Front Vet Sci 2021 3;8:726723. Epub 2021 Sep 3.

Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

Since 2016, a novel porcine circovirus, PCV3, has been infecting pigs, causing significant economic losses to the pig industry. In recent years, the infection rate of PCV3 has been increasing, and thus rapid and accurate detection methods for PCV3 are essential. In this study, we established a novel probe-based single multiple cross displacement amplification (P-S-MCDA) method for PCV3. Read More

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September 2021

Molecular assemblies on surfaces: towards physical and electronic decoupling of organic molecules.

Beilstein J Nanotechnol 2021 23;12:950-956. Epub 2021 Aug 23.

Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands.

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Evaluation of the visibility of peri-implant bone defects using ultrasonography with two types of probes.

J Ultrason 2021 Aug 9;21(86):e206-e212. Epub 2021 Sep 9.

Department of Dentomaxillofacial Radiology, Ankara University, Faculty of Dentistry, Turkey.

The aim of the present study was to evaluate the efficacy of intraoral and extraoral ultrasonography evaluations performed with two different types of probes (linear and "hockey stick") for the visibility of peri-implant bone defects. Fourteen implants were inserted into sheep heads. Peri-implant bone defects were created without knowing the depth, which served as the gold standard for the defects. Read More

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Application of evidence-based nursing in patients after cataract surgery and its impacts on visual acuity recovery and psychological status.

Authors:
Xin Li

Am J Transl Res 2021 15;13(8):9784-9789. Epub 2021 Aug 15.

Department of Ophthalmology, Xiyuan Hospital, China Academy of Chinese Medical Science Beijing, China.

Objective: To probe into the evidence-based nursing in patients after cataract surgery and its impact on their visual acuity recovery and psychological status.

Methods: Ninety-seven patients with cataract who underwent surgery in our hospital were randomly divided into control group (n=48, receiving conventional nursing) and observation group (n=49, receiving evidence-based nursing) for a prospective study. Postoperative visual acuity recovery, incidence of complications, recurrence rate within 1 year, psychological status before and after surgery and quality of life were compared between the two groups. Read More

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